HOT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HOT1
Entrez Gene ID	855208
Full Name	Hot1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182677.1	NP_013895.1	Hot1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results presented in this work demonstrate that Hot1 enrichment is not detected through the coding regions of its target genes and rule out a direct role in transcription elongation
Title: Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes.

the C-terminal region, which corresponds to amino acids 610-719, is the DNA-binding domain of Hot1, which recognizes sequence 5'-GGGACAAA-3' located in the promoter of gene STL1. Similar sequences are found in the promoters of other Hot1 targets.
Title: The C-terminal region of the Hot1 transcription factor binds GGGACAAA-related sequences in the promoter of its target genes.

Hot1 is essential for transcription of just one gene, STL1. Hot1 may represent a class of transcription factors that are essential for transcription of a very few genes or even just one.
Title: The yeast Hot1 transcription factor is critical for activating a single target gene, STL1.

Single-cell gene expression analysis reveals altered activation of Hot1-targeted STL1 in ck2 mutants, resulting in a bimodal to unimodal shift in expression.
Title: Casein kinase II regulation of the Hot1 transcription factor promotes stochastic gene expression.

Expression of Yhr087wp under high glucose concentration is controlled by Hot1p and Sko1p transcription factors, which bind to its promoter.
Title: The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress.

The following HOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04167	NM_001182677.1 Latest version!	Saccharomyces cerevisiae S288C Hot1p (HOT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HOT1 ( NM_001182677.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013895.1 Saccharomyces cerevisiae S288C Hot1p (HOT1), partial mRNA.

Price & Availability↑

CloneID	OSi04167
Clone ID Related Accession (Same CDS sequence)	NM_001182677.1
Accession Version	NM_001182677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Hot1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGGAA TGGGTATTGC GATTCTTTGC ATCGTACGTA CAAAGATTTA TAGGATAACC 
ATCAGCTTCG ATTATTCTAC ACTGATGAGC CCTTTCTTCT TATTCTTAAT GATGCCCACT 
ACATTAAAGG ATGGGTACAG GATGAATAGT CAAGTAAATG AAGATGCTAT AGGTATCAAT 
CTTGACCTCT CACTGCCCAC TCACATTTCG CCTACGACTG GTTCAGAGAG CGCCAGTGGC 
AGTAACGCCA GTACACTTCG CAACGATGGA AATGCACTAG ATGGAGGTCT TTTGAGGACT 
TCAGCAGCGA TTTCAGCACC CACAGGTACC AGTCAACCAA CAGAAACTAT TGGTGAAAAA 
CTGAGTAATG AAGAGCGGGT GAACTCGAAT GTTTCCGCTT CGAACTCGAC AACGGCGGGT 
ACAGGGCGTA TGTTAAGCCA ATCCTTGACT AATGATTCAC CGTCGAACGA AATATCGACG 
GATCAGTTGA AGATTTTTCA AAGAATGGAC GAAATGAGTG CCCGAATGAT TGAGATGGAG 
GAAAGCTTCA ACAAGTTATC TAATAAGATC GCGGAACAGA ATACAATGGT TTTAAACCTA 
AAACAAGATA ACTATAAAGT TATGAACAAA TTGAATATAT TACTTAAGTT GGTTGCGCAG 
CCAAGCGCGC GCCCAAGCAC AAACAATGCC CAAAATAAGC TTGCTATCGA ATTGCTAAAT 
TCGATATCAG CCGTTAGTAG TGCTTACTTG CAGAAAATGC AGAATAATGG CTCTGGTAGA 
CAGCATACTG CTGATTTATG TACAGGAGAT TCCAACACCC ATTCTGGTAT CAACCAGCAT 
CGGACTACGA ATGGAACCAT AGATGTGAAT ACGAATACTG CACAGCTAAA CAACCAATTT 
TCTAACGCAC TAAATACTAT CTTACCTGAT CAGCAGCATA ATCGAAATAA TGTTTCCCAA 
AATATAAACC AATCGCTCCC TAACCGACAA CTTGGTCCTG TTATAAACAC ACAAGCCAAT 
CAGAACCAGT CGCAGGTTTT AATTCATAAC ACAAATACAC ATCAGCAGGT AAATCGCTCG 
CCTATTAGTT TCCCGAACGC TTCAACTGAC AAGCCATTCA AGTTGAACCC AAATGGGATT 
AAACGGCGCA GAAGAAATAC GCAAAGTAAC AACAATGCGA GCACAAATGA CCATGCTAGT 
GCTGCACAAA AGCCCATATC AGCCCTATCT CCATTAACGA ATTCTCATAA TTCTACAACT 
AGCATGAATT ATACCAATTC TTCCATTCAT TCAGGCGTCA CCAGTGCTTC AAATTCATTT 
CATGACTTAA ATTCATTGAA TAACTTTGGC ACGACAACAG CTTTAAGTTT GCCCAGCTTA 
GCCCTAGATA ACGCTTCTTT TCCCCCAAAT CAAAATGTTA TACCCCCTAT TATAAATAAT 
ACCCAGCAGC CTCTATCATT TTCCCAATTG ATCAATCAGG ATAGCACGAC ATCTGAATTG 
TTACCGAGTG GTAAGTCAGG GGTGAACACG AATATTGTAA ATAGAAACAG AGCTTCTACT 
CTACCGTCGT ACCCAAAACC AATGACAGTG AAAAGTAATG TCGACGATGA TGGCTATCAA 
GAGGATGATG ATGATGACGG TGATGACGAA GGTGATGGAA GGGATAATGA AGAAGACAGC 
ACTGCTGAGG AAGATGAAGT AGATGATGAA ATCGAAACAG ATATGAAGAA CGCTTCGATT 
AACAAGCGGA GGAGGAGCTT GCATCATAAA AAAAGTAACT CTTTAAACGG TAGAAGAAAG 
CTTCACGGCG AGTCGGCTAC TAAGCCCAAC ATAAACTCTG ATTTACATTA TAGGATATTG 
AAGGCTCCCA CTGACGTAAA GACTATATGG GAAGAGTATG ATACTGGAAT AAGAGGAAAG 
CCATCGATCA AGCATCTTGA GGCCAAGTAT GGTAACAAGT GGAGGCTCAA TAAAAATAAG 
AAGACCTTTT CAAGGCGAAA AAGATTGTAC AAGTTCATTC TGAACGGTAT GGAGAGAGGT 
AAAACGGCAC AAGAGATGAT AGAAACCTTA GAGAACAAAA GGCTATATAA AGATGATGAG 
GATGGCGAAG TAAAGAAAAG AACCATTGGC TGGTTGCAAG AGAGCCTTGC TGGAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013895.1
CDS	1..2160
Translation	MSGMGIAILCIVRTKIYRITISFDYSTLMSPFFLFLMMPTTLKDGYRMNSQVNEDAIGINLDLSLPTHISPTTGSESASGSNASTLRNDGNALDGGLLRTSAAISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEISTDQLKIFQRMDEMSARMIEMEESFNKLSNKIAEQNTMVLNLKQDNYKVMNKLNILLKLVAQPSARPSTNNAQNKLAIELLNSISAVSSAYLQKMQNNGSGRQHTADLCTGDSNTHSGINQHRTTNGTIDVNTNTAQLNNQFSNALNTILPDQQHNRNNVSQNINQSLPNRQLGPVINTQANQNQSQVLIHNTNTHQQVNRSPISFPNASTDKPFKLNPNGIKRRRRNTQSNNNASTNDHASAAQKPISALSPLTNSHNSTTSMNYTNSSIHSGVTSASNSFHDLNSLNNFGTTTALSLPSLALDNASFPPNQNVIPPIINNTQQPLSFSQLINQDSTTSELLPSGKSGVNTNIVNRNRASTLPSYPKPMTVKSNVDDDGYQEDDDDDGDDEGDGRDNEEDSTAEEDEVDDEIETDMKNASINKRRRSLHHKKSNSLNGRRKLHGESATKPNINSDLHYRILKAPTDVKTIWEEYDTGIRGKPSIKHLEAKYGNKWRLNKNKKTFSRRKRLYKFILNGMERGKTAQEMIETLENKRLYKDDEDGEVKKRTIGWLQESLAGI

Target ORF information:

RefSeq Version	NM_001182677.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Hot1p (HOT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182677.1

ORF Insert Sequence:

ATGTCTGGAA TGGGTATTGC GATTCTTTGC ATCGTACGTA CAAAGATTTA TAGGATAACC 
ATCAGCTTCG ATTATTCTAC ACTGATGAGC CCTTTCTTCT TATTCTTAAT GATGCCCACT 
ACATTAAAGG ATGGGTACAG GATGAATAGT CAAGTAAATG AAGATGCTAT AGGTATCAAT 
CTTGACCTCT CACTGCCCAC TCACATTTCG CCTACGACTG GTTCAGAGAG CGCCAGTGGC 
AGTAACGCCA GTACACTTCG CAACGATGGA AATGCACTAG ATGGAGGTCT TTTGAGGACT 
TCAGCAGCGA TTTCAGCACC CACAGGTACC AGTCAACCAA CAGAAACTAT TGGTGAAAAA 
CTGAGTAATG AAGAGCGGGT GAACTCGAAT GTTTCCGCTT CGAACTCGAC AACGGCGGGT 
ACAGGGCGTA TGTTAAGCCA ATCCTTGACT AATGATTCAC CGTCGAACGA AATATCGACG 
GATCAGTTGA AGATTTTTCA AAGAATGGAC GAAATGAGTG CCCGAATGAT TGAGATGGAG 
GAAAGCTTCA ACAAGTTATC TAATAAGATC GCGGAACAGA ATACAATGGT TTTAAACCTA 
AAACAAGATA ACTATAAAGT TATGAACAAA TTGAATATAT TACTTAAGTT GGTTGCGCAG 
CCAAGCGCGC GCCCAAGCAC AAACAATGCC CAAAATAAGC TTGCTATCGA ATTGCTAAAT 
TCGATATCAG CCGTTAGTAG TGCTTACTTG CAGAAAATGC AGAATAATGG CTCTGGTAGA 
CAGCATACTG CTGATTTATG TACAGGAGAT TCCAACACCC ATTCTGGTAT CAACCAGCAT 
CGGACTACGA ATGGAACCAT AGATGTGAAT ACGAATACTG CACAGCTAAA CAACCAATTT 
TCTAACGCAC TAAATACTAT CTTACCTGAT CAGCAGCATA ATCGAAATAA TGTTTCCCAA 
AATATAAACC AATCGCTCCC TAACCGACAA CTTGGTCCTG TTATAAACAC ACAAGCCAAT 
CAGAACCAGT CGCAGGTTTT AATTCATAAC ACAAATACAC ATCAGCAGGT AAATCGCTCG 
CCTATTAGTT TCCCGAACGC TTCAACTGAC AAGCCATTCA AGTTGAACCC AAATGGGATT 
AAACGGCGCA GAAGAAATAC GCAAAGTAAC AACAATGCGA GCACAAATGA CCATGCTAGT 
GCTGCACAAA AGCCCATATC AGCCCTATCT CCATTAACGA ATTCTCATAA TTCTACAACT 
AGCATGAATT ATACCAATTC TTCCATTCAT TCAGGCGTCA CCAGTGCTTC AAATTCATTT 
CATGACTTAA ATTCATTGAA TAACTTTGGC ACGACAACAG CTTTAAGTTT GCCCAGCTTA 
GCCCTAGATA ACGCTTCTTT TCCCCCAAAT CAAAATGTTA TACCCCCTAT TATAAATAAT 
ACCCAGCAGC CTCTATCATT TTCCCAATTG ATCAATCAGG ATAGCACGAC ATCTGAATTG 
TTACCGAGTG GTAAGTCAGG GGTGAACACG AATATTGTAA ATAGAAACAG AGCTTCTACT 
CTACCGTCGT ACCCAAAACC AATGACAGTG AAAAGTAATG TCGACGATGA TGGCTATCAA 
GAGGATGATG ATGATGACGG TGATGACGAA GGTGATGGAA GGGATAATGA AGAAGACAGC 
ACTGCTGAGG AAGATGAAGT AGATGATGAA ATCGAAACAG ATATGAAGAA CGCTTCGATT 
AACAAGCGGA GGAGGAGCTT GCATCATAAA AAAAGTAACT CTTTAAACGG TAGAAGAAAG 
CTTCACGGCG AGTCGGCTAC TAAGCCCAAC ATAAACTCTG ATTTACATTA TAGGATATTG 
AAGGCTCCCA CTGACGTAAA GACTATATGG GAAGAGTATG ATACTGGAAT AAGAGGAAAG 
CCATCGATCA AGCATCTTGA GGCCAAGTAT GGTAACAAGT GGAGGCTCAA TAAAAATAAG 
AAGACCTTTT CAAGGCGAAA AAGATTGTAC AAGTTCATTC TGAACGGTAT GGAGAGAGGT 
AAAACGGCAC AAGAGATGAT AGAAACCTTA GAGAACAAAA GGCTATATAA AGATGATGAG 
GATGGCGAAG TAAAGAAAAG AACCATTGGC TGGTTGCAAG AGAGCCTTGC TGGAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HOT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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