ORF » Species Summary » Saccharomyces cerevisiae S288C » HOT1 cDNA ORF clone

HOT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following HOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04167 NM_001182677.1
Latest version!		 Saccharomyces cerevisiae S288C Hot1p (HOT1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    HOT1 ( NM_001182677.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013895.1 Saccharomyces cerevisiae S288C Hot1p (HOT1), partial mRNA.
    Price & Availability↑
    CloneID OSi04167
    Clone ID Related Accession (Same CDS sequence) NM_001182677.1
    Accession Version NM_001182677.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Hot1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCTGGAA TGGGTATTGC GATTCTTTGC ATCGTACGTA CAAAGATTTA TAGGATAACC 
    ATCAGCTTCG ATTATTCTAC ACTGATGAGC CCTTTCTTCT TATTCTTAAT GATGCCCACT 
    ACATTAAAGG ATGGGTACAG GATGAATAGT CAAGTAAATG AAGATGCTAT AGGTATCAAT 
    CTTGACCTCT CACTGCCCAC TCACATTTCG CCTACGACTG GTTCAGAGAG CGCCAGTGGC 
    AGTAACGCCA GTACACTTCG CAACGATGGA AATGCACTAG ATGGAGGTCT TTTGAGGACT 
    TCAGCAGCGA TTTCAGCACC CACAGGTACC AGTCAACCAA CAGAAACTAT TGGTGAAAAA 
    CTGAGTAATG AAGAGCGGGT GAACTCGAAT GTTTCCGCTT CGAACTCGAC AACGGCGGGT 
    ACAGGGCGTA TGTTAAGCCA ATCCTTGACT AATGATTCAC CGTCGAACGA AATATCGACG 
    GATCAGTTGA AGATTTTTCA AAGAATGGAC GAAATGAGTG CCCGAATGAT TGAGATGGAG 
    GAAAGCTTCA ACAAGTTATC TAATAAGATC GCGGAACAGA ATACAATGGT TTTAAACCTA 
    AAACAAGATA ACTATAAAGT TATGAACAAA TTGAATATAT TACTTAAGTT GGTTGCGCAG 
    CCAAGCGCGC GCCCAAGCAC AAACAATGCC CAAAATAAGC TTGCTATCGA ATTGCTAAAT 
    TCGATATCAG CCGTTAGTAG TGCTTACTTG CAGAAAATGC AGAATAATGG CTCTGGTAGA 
    CAGCATACTG CTGATTTATG TACAGGAGAT TCCAACACCC ATTCTGGTAT CAACCAGCAT 
    CGGACTACGA ATGGAACCAT AGATGTGAAT ACGAATACTG CACAGCTAAA CAACCAATTT 
    TCTAACGCAC TAAATACTAT CTTACCTGAT CAGCAGCATA ATCGAAATAA TGTTTCCCAA 
    AATATAAACC AATCGCTCCC TAACCGACAA CTTGGTCCTG TTATAAACAC ACAAGCCAAT 
    CAGAACCAGT CGCAGGTTTT AATTCATAAC ACAAATACAC ATCAGCAGGT AAATCGCTCG 
    CCTATTAGTT TCCCGAACGC TTCAACTGAC AAGCCATTCA AGTTGAACCC AAATGGGATT 
    AAACGGCGCA GAAGAAATAC GCAAAGTAAC AACAATGCGA GCACAAATGA CCATGCTAGT 
    GCTGCACAAA AGCCCATATC AGCCCTATCT CCATTAACGA ATTCTCATAA TTCTACAACT 
    AGCATGAATT ATACCAATTC TTCCATTCAT TCAGGCGTCA CCAGTGCTTC AAATTCATTT 
    CATGACTTAA ATTCATTGAA TAACTTTGGC ACGACAACAG CTTTAAGTTT GCCCAGCTTA 
    GCCCTAGATA ACGCTTCTTT TCCCCCAAAT CAAAATGTTA TACCCCCTAT TATAAATAAT 
    ACCCAGCAGC CTCTATCATT TTCCCAATTG ATCAATCAGG ATAGCACGAC ATCTGAATTG 
    TTACCGAGTG GTAAGTCAGG GGTGAACACG AATATTGTAA ATAGAAACAG AGCTTCTACT 
    CTACCGTCGT ACCCAAAACC AATGACAGTG AAAAGTAATG TCGACGATGA TGGCTATCAA 
    GAGGATGATG ATGATGACGG TGATGACGAA GGTGATGGAA GGGATAATGA AGAAGACAGC 
    ACTGCTGAGG AAGATGAAGT AGATGATGAA ATCGAAACAG ATATGAAGAA CGCTTCGATT 
    AACAAGCGGA GGAGGAGCTT GCATCATAAA AAAAGTAACT CTTTAAACGG TAGAAGAAAG 
    CTTCACGGCG AGTCGGCTAC TAAGCCCAAC ATAAACTCTG ATTTACATTA TAGGATATTG 
    AAGGCTCCCA CTGACGTAAA GACTATATGG GAAGAGTATG ATACTGGAAT AAGAGGAAAG 
    CCATCGATCA AGCATCTTGA GGCCAAGTAT GGTAACAAGT GGAGGCTCAA TAAAAATAAG 
    AAGACCTTTT CAAGGCGAAA AAGATTGTAC AAGTTCATTC TGAACGGTAT GGAGAGAGGT 
    AAAACGGCAC AAGAGATGAT AGAAACCTTA GAGAACAAAA GGCTATATAA AGATGATGAG 
    GATGGCGAAG TAAAGAAAAG AACCATTGGC TGGTTGCAAG AGAGCCTTGC TGGAATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_013895.1
    CDS1..2160
    Translation

    Target ORF information:

    RefSeq Version NM_001182677.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Hot1p (HOT1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001182677.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCTGGAA TGGGTATTGC GATTCTTTGC ATCGTACGTA CAAAGATTTA TAGGATAACC 
    ATCAGCTTCG ATTATTCTAC ACTGATGAGC CCTTTCTTCT TATTCTTAAT GATGCCCACT 
    ACATTAAAGG ATGGGTACAG GATGAATAGT CAAGTAAATG AAGATGCTAT AGGTATCAAT 
    CTTGACCTCT CACTGCCCAC TCACATTTCG CCTACGACTG GTTCAGAGAG CGCCAGTGGC 
    AGTAACGCCA GTACACTTCG CAACGATGGA AATGCACTAG ATGGAGGTCT TTTGAGGACT 
    TCAGCAGCGA TTTCAGCACC CACAGGTACC AGTCAACCAA CAGAAACTAT TGGTGAAAAA 
    CTGAGTAATG AAGAGCGGGT GAACTCGAAT GTTTCCGCTT CGAACTCGAC AACGGCGGGT 
    ACAGGGCGTA TGTTAAGCCA ATCCTTGACT AATGATTCAC CGTCGAACGA AATATCGACG 
    GATCAGTTGA AGATTTTTCA AAGAATGGAC GAAATGAGTG CCCGAATGAT TGAGATGGAG 
    GAAAGCTTCA ACAAGTTATC TAATAAGATC GCGGAACAGA ATACAATGGT TTTAAACCTA 
    AAACAAGATA ACTATAAAGT TATGAACAAA TTGAATATAT TACTTAAGTT GGTTGCGCAG 
    CCAAGCGCGC GCCCAAGCAC AAACAATGCC CAAAATAAGC TTGCTATCGA ATTGCTAAAT 
    TCGATATCAG CCGTTAGTAG TGCTTACTTG CAGAAAATGC AGAATAATGG CTCTGGTAGA 
    CAGCATACTG CTGATTTATG TACAGGAGAT TCCAACACCC ATTCTGGTAT CAACCAGCAT 
    CGGACTACGA ATGGAACCAT AGATGTGAAT ACGAATACTG CACAGCTAAA CAACCAATTT 
    TCTAACGCAC TAAATACTAT CTTACCTGAT CAGCAGCATA ATCGAAATAA TGTTTCCCAA 
    AATATAAACC AATCGCTCCC TAACCGACAA CTTGGTCCTG TTATAAACAC ACAAGCCAAT 
    CAGAACCAGT CGCAGGTTTT AATTCATAAC ACAAATACAC ATCAGCAGGT AAATCGCTCG 
    CCTATTAGTT TCCCGAACGC TTCAACTGAC AAGCCATTCA AGTTGAACCC AAATGGGATT 
    AAACGGCGCA GAAGAAATAC GCAAAGTAAC AACAATGCGA GCACAAATGA CCATGCTAGT 
    GCTGCACAAA AGCCCATATC AGCCCTATCT CCATTAACGA ATTCTCATAA TTCTACAACT 
    AGCATGAATT ATACCAATTC TTCCATTCAT TCAGGCGTCA CCAGTGCTTC AAATTCATTT 
    CATGACTTAA ATTCATTGAA TAACTTTGGC ACGACAACAG CTTTAAGTTT GCCCAGCTTA 
    GCCCTAGATA ACGCTTCTTT TCCCCCAAAT CAAAATGTTA TACCCCCTAT TATAAATAAT 
    ACCCAGCAGC CTCTATCATT TTCCCAATTG ATCAATCAGG ATAGCACGAC ATCTGAATTG 
    TTACCGAGTG GTAAGTCAGG GGTGAACACG AATATTGTAA ATAGAAACAG AGCTTCTACT 
    CTACCGTCGT ACCCAAAACC AATGACAGTG AAAAGTAATG TCGACGATGA TGGCTATCAA 
    GAGGATGATG ATGATGACGG TGATGACGAA GGTGATGGAA GGGATAATGA AGAAGACAGC 
    ACTGCTGAGG AAGATGAAGT AGATGATGAA ATCGAAACAG ATATGAAGAA CGCTTCGATT 
    AACAAGCGGA GGAGGAGCTT GCATCATAAA AAAAGTAACT CTTTAAACGG TAGAAGAAAG 
    CTTCACGGCG AGTCGGCTAC TAAGCCCAAC ATAAACTCTG ATTTACATTA TAGGATATTG 
    AAGGCTCCCA CTGACGTAAA GACTATATGG GAAGAGTATG ATACTGGAAT AAGAGGAAAG 
    CCATCGATCA AGCATCTTGA GGCCAAGTAT GGTAACAAGT GGAGGCTCAA TAAAAATAAG 
    AAGACCTTTT CAAGGCGAAA AAGATTGTAC AAGTTCATTC TGAACGGTAT GGAGAGAGGT 
    AAAACGGCAC AAGAGATGAT AGAAACCTTA GAGAACAAAA GGCTATATAA AGATGATGAG 
    GATGGCGAAG TAAAGAAAAG AACCATTGGC TGGTTGCAAG AGAGCCTTGC TGGAATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
    Nature387(6632 Suppl)90-3(1997 May)
    Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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