EPO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	EPO1
Entrez Gene ID	855154
Full Name	Epo1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182625.1	NP_013843.1	Epo1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We find that Kss1 also localizes to the sites of polarized growth and Bem3 interacts with Kss1 at the septin-ring assembly site. TD2 localization is largely mediated by its interaction with the polarisome component Epo1 via heterotypic coiled-coil interaction. This finding reveals a novel role for the polarisome in linking Bem3 to its functional target,Cdc42.
Title: Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast.

Identify Epo1p as a novel and important component of the polarisome that promotes cortical endoplasmic reticulum tethering at sites of polarized growth in budding yeast.
Title: A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip.

Study shows that a direct interaction between the endoplasmic reticulum (ER) protein Scs2 and the septin Shs1 creates the diffusion barrier in yeast. Barrier formation requires Epo1, a novel ER-associated subunit of the polarisome that interacts with Scs2 and Shs1, encoded by the ORF YMR124W.
Title: Polarization of the endoplasmic reticulum by ER-septin tethering.

The following EPO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04120	NM_001182625.1 Latest version!	Saccharomyces cerevisiae S288C Epo1p (EPO1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPO1 ( NM_001182625.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013843.1 Saccharomyces cerevisiae S288C Epo1p (EPO1), partial mRNA.

Price & Availability↑

CloneID	OSi04120
Clone ID Related Accession (Same CDS sequence)	NM_001182625.1
Accession Version	NM_001182625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Epo1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGCAG GTTTATCGAC CATGGCAACA AGAAACGGTC AGTCTTCTGC TAGAGTTAAA 
TTGAGAAATA ACCTCTTGAA TAATGATATA GGAAATATTG ACATTAGGGA TGAAACACCT 
ATTTCAAGGA ACGGGAATGA CAGCAATATT AACATCCAGC CTTCTTCCGT GCCGCAGCAG 
CAGCAGCAGC AGCAGCAATA CTACCGTAAT GGCATGAATG AGGCTCCAAT ACAAGCGCCA 
CTTCAACAAC GTCAAATTCC CATGCAGAAT TATTCACAAC AGCAACGCCA GCAACAACAG 
TATAATTTTG AGTATTCCAA TCCACATATG AATGAGATTC CCCTCATGCA GCATAACTTC 
ACCAAACCAT CACTAAGCAA TAATCGCGAT AATGTTAATG GTAAGAAAGC TTCCTCATTT 
ACCCAGAGCT CATTTTCCAA TTTTTTCAAA CATAAGCATC AATTTGGGAA GTCAAAGAAA 
AACACTAAGG GGACAGGAGG TGGTGGAGAT GGTGATGATG ACGATGAGGT TATCTTGGAT 
GATTCTGCAA ACTCCGACCT AACATTCAAT GACATTCAGA CCTTCGGTCA CAAGGGCGGT 
GACAAATATG GATATGGTGG TGATTCCACT CCTATCATTC CAACATTGGT GACTAAAGAC 
CGCGGCAATA TGAGTAATAC TGAGTATAGG AAGTACATTA CTAATCAAAG GAAAACCGCA 
ATGAATGCCA TGGCTAAACA AACTAAAAAT GGTACATTAG CCTCGTTGCC TCCGAGAGCA 
ATGTCTTTGC AAAGTTTCCC GAATGGTAAT CCTTTAATGC AAGCACCCAC TCCTCATCCT 
AGATTTCAAC CGAATAAAAT GGTGAGCGCT AATTATTCGA GATCAAATTC GTTGATGAGT 
GGACCTCCCG GACAATTTCG ACAGCCGCAG CAACAAAGAA TGCTACCGAT GAACAACTAC 
AACAATCACC CAGGTCAATT CCAGAATACC CCACCAGTAA TGCCCTCTGG ACAACAGCCA 
CCTCAGCAAC CACGTACTCT GTCATTAACT AATGGCCCAA GATATTCACC CCAAAATCCT 
CGGCCATTCG CAGGTCATCA GCAGATATCA CAAAGACAAC AACAACAACA ACAACAACTT 
CAACTTCATC CAATGTCTGA AGGCTATCGG ACAATGTCTC TACAAAGTCA GAACGTTCCC 
CAAGGGTTTA ATCCATGGTC ACCAAATGAC AACGATCGTA AAGCTGTGTC CATGAAACAG 
CCAATCAGTC AATCGTCAAT TTCCTCAAAG AATAATTCAG CTTATTCAAT ACCGAACGTA 
CAAAACAACT CCCTGACTAC ATTCTCTCCG TCATCTCCTA CTGATGCTAC AGCAATGCCT 
AACTCTACAA AACAAGGATC TTCACCACTA AAAAAACAAG TGAACATTGA TCAACCTATT 
GAAAACAAGG GAAAACTGAA TGTTTTGCAA TTATCCACTC CTCAACAAAA CGAGTTAAAG 
GAAAAAGAAA GGAAGCTAGC TGAAATGGAA AAATCTTTAC GTGAGAGAGA AGCACTAGTA 
GAAGAAAAAG AAAAGGAAAG AGCTGAGAAA AATACTGAAG CAAATGAAGA GGAGGAAATT 
TCACATGAAT CAGACGATTT AAATTTAAGA CCTGCGAGTG CGCTAGAGAC TGGTTTAAAA 
GATCTTAAAT TGGAAAGTGA AAGTGCAGTC GCCAATAGAG CGTCCTTGTC GACTTTCTCA 
TCCACATTTA GTGATTCTCC CTCCAAGCAA AGAATCATCA ATACCAGAAC AGGTATGTAT 
AAGCTAGAAA ACTCTACTGA TATCAATGAA TACGTGACCG CACAAGAATT CCCCTCTCCC 
GGAAAGTATA ACTCAAATTC GGATAATGGC GAAATGAATA CAACGAACGA GGTCGATTTT 
GATTTCAATA CTAGTAAAAG GGCTTCTTTG TTACAATCCA TACCGGAAAG AGATCCCAAA 
AGAAATGTCT CAGATGCTAC CATTAAGCGA CGTGAATCCG ATGGCAATGG CAGGAGACTA 
AGTAATGTCA ACATATCCAT GAACCAGGAA AACATCAACA ATGATACTTT TCTATATAAA 
AAAAATAATC GCGATGGTCA TTTATCAGCA GTTTCTCATA TGTCCAGCTC TTCCCGTAGA 
TCGTTCATCT CCAATACGCT GCCGTTGAAT ATTGATTCCG CCTCAGAATC TGATAATTTT 
GTACCGCATA TGGATGGTTC ACCCTCCAAA ACAAAATCAG CCCCAGTTAG CTACGATAAG 
GATGGCATGA ATGCTTCAGA AGAAGACTTC AGTTTTGATA ATACTTTAGC CAAACCGTAT 
GAACCGTTAT ATGCTAGGAG AGGAGACATA ACTTCCGCCG GATCAACTAG TGGAGAAGAT 
AGCTCTCAGC CAAAGATGAT AACAATATCT GGTGAGCAAT TGAATTTGAT AACAGAAAAC 
AAAGAATTAA TGAATGAGTT GACACTGGTG TCTACAGAAT TAGCAGAATC GATAAAAAGG 
GAAACTGAAT TAGAAGAAAG AATACGGCTG TATGAAACCA ATAATTCAGC ACCATCTTTT 
GACGATTCTT CGAGTGTGTC TTTTTCTGAC TTTGAAAAGG AACTGAGAAA AAAATCCAGT 
AAAATTGTGC AATTGATCCA GCAATTAAAT GACGAGAGAT TAAAGAGATT CATTGCGGAG 
GAGCAGTTAT TACTCCAAGA AAATGGCACC AAACCAAGTT CTATGGAACT AGTAGGAAGA 
ATTGAAAACT TGAATAAACT TATTGATGAG AGAGATTCAG AAATTGAAAT GCTTAAAGGA 
CGTTTACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013843.1
CDS	1..2832
Translation	MDAGLSTMATRNGQSSARVKLRNNLLNNDIGNIDIRDETPISRNGNDSNINIQPSSVPQQQQQQQQYYRNGMNEAPIQAPLQQRQIPMQNYSQQQRQQQQYNFEYSNPHMNEIPLMQHNFTKPSLSNNRDNVNGKKASSFTQSSFSNFFKHKHQFGKSKKNTKGTGGGGDGDDDDEVILDDSANSDLTFNDIQTFGHKGGDKYGYGGDSTPIIPTLVTKDRGNMSNTEYRKYITNQRKTAMNAMAKQTKNGTLASLPPRAMSLQSFPNGNPLMQAPTPHPRFQPNKMVSANYSRSNSLMSGPPGQFRQPQQQRMLPMNNYNNHPGQFQNTPPVMPSGQQPPQQPRTLSLTNGPRYSPQNPRPFAGHQQISQRQQQQQQQLQLHPMSEGYRTMSLQSQNVPQGFNPWSPNDNDRKAVSMKQPISQSSISSKNNSAYSIPNVQNNSLTTFSPSSPTDATAMPNSTKQGSSPLKKQVNIDQPIENKGKLNVLQLSTPQQNELKEKERKLAEMEKSLREREALVEEKEKERAEKNTEANEEEEISHESDDLNLRPASALETGLKDLKLESESAVANRASLSTFSSTFSDSPSKQRIINTRTGMYKLENSTDINEYVTAQEFPSPGKYNSNSDNGEMNTTNEVDFDFNTSKRASLLQSIPERDPKRNVSDATIKRRESDGNGRRLSNVNISMNQENINNDTFLYKKNNRDGHLSAVSHMSSSSRRSFISNTLPLNIDSASESDNFVPHMDGSPSKTKSAPVSYDKDGMNASEEDFSFDNTLAKPYEPLYARRGDITSAGSTSGEDSSQPKMITISGEQLNLITENKELMNELTLVSTELAESIKRETELEERIRLYETNNSAPSFDDSSSVSFSDFEKELRKKSSKIVQLIQQLNDERLKRFIAEEQLLLQENGTKPSSMELVGRIENLNKLIDERDSEIEMLKGRLQ

Target ORF information:

RefSeq Version	NM_001182625.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Epo1p (EPO1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182625.1

ORF Insert Sequence:

ATGGATGCAG GTTTATCGAC CATGGCAACA AGAAACGGTC AGTCTTCTGC TAGAGTTAAA 
TTGAGAAATA ACCTCTTGAA TAATGATATA GGAAATATTG ACATTAGGGA TGAAACACCT 
ATTTCAAGGA ACGGGAATGA CAGCAATATT AACATCCAGC CTTCTTCCGT GCCGCAGCAG 
CAGCAGCAGC AGCAGCAATA CTACCGTAAT GGCATGAATG AGGCTCCAAT ACAAGCGCCA 
CTTCAACAAC GTCAAATTCC CATGCAGAAT TATTCACAAC AGCAACGCCA GCAACAACAG 
TATAATTTTG AGTATTCCAA TCCACATATG AATGAGATTC CCCTCATGCA GCATAACTTC 
ACCAAACCAT CACTAAGCAA TAATCGCGAT AATGTTAATG GTAAGAAAGC TTCCTCATTT 
ACCCAGAGCT CATTTTCCAA TTTTTTCAAA CATAAGCATC AATTTGGGAA GTCAAAGAAA 
AACACTAAGG GGACAGGAGG TGGTGGAGAT GGTGATGATG ACGATGAGGT TATCTTGGAT 
GATTCTGCAA ACTCCGACCT AACATTCAAT GACATTCAGA CCTTCGGTCA CAAGGGCGGT 
GACAAATATG GATATGGTGG TGATTCCACT CCTATCATTC CAACATTGGT GACTAAAGAC 
CGCGGCAATA TGAGTAATAC TGAGTATAGG AAGTACATTA CTAATCAAAG GAAAACCGCA 
ATGAATGCCA TGGCTAAACA AACTAAAAAT GGTACATTAG CCTCGTTGCC TCCGAGAGCA 
ATGTCTTTGC AAAGTTTCCC GAATGGTAAT CCTTTAATGC AAGCACCCAC TCCTCATCCT 
AGATTTCAAC CGAATAAAAT GGTGAGCGCT AATTATTCGA GATCAAATTC GTTGATGAGT 
GGACCTCCCG GACAATTTCG ACAGCCGCAG CAACAAAGAA TGCTACCGAT GAACAACTAC 
AACAATCACC CAGGTCAATT CCAGAATACC CCACCAGTAA TGCCCTCTGG ACAACAGCCA 
CCTCAGCAAC CACGTACTCT GTCATTAACT AATGGCCCAA GATATTCACC CCAAAATCCT 
CGGCCATTCG CAGGTCATCA GCAGATATCA CAAAGACAAC AACAACAACA ACAACAACTT 
CAACTTCATC CAATGTCTGA AGGCTATCGG ACAATGTCTC TACAAAGTCA GAACGTTCCC 
CAAGGGTTTA ATCCATGGTC ACCAAATGAC AACGATCGTA AAGCTGTGTC CATGAAACAG 
CCAATCAGTC AATCGTCAAT TTCCTCAAAG AATAATTCAG CTTATTCAAT ACCGAACGTA 
CAAAACAACT CCCTGACTAC ATTCTCTCCG TCATCTCCTA CTGATGCTAC AGCAATGCCT 
AACTCTACAA AACAAGGATC TTCACCACTA AAAAAACAAG TGAACATTGA TCAACCTATT 
GAAAACAAGG GAAAACTGAA TGTTTTGCAA TTATCCACTC CTCAACAAAA CGAGTTAAAG 
GAAAAAGAAA GGAAGCTAGC TGAAATGGAA AAATCTTTAC GTGAGAGAGA AGCACTAGTA 
GAAGAAAAAG AAAAGGAAAG AGCTGAGAAA AATACTGAAG CAAATGAAGA GGAGGAAATT 
TCACATGAAT CAGACGATTT AAATTTAAGA CCTGCGAGTG CGCTAGAGAC TGGTTTAAAA 
GATCTTAAAT TGGAAAGTGA AAGTGCAGTC GCCAATAGAG CGTCCTTGTC GACTTTCTCA 
TCCACATTTA GTGATTCTCC CTCCAAGCAA AGAATCATCA ATACCAGAAC AGGTATGTAT 
AAGCTAGAAA ACTCTACTGA TATCAATGAA TACGTGACCG CACAAGAATT CCCCTCTCCC 
GGAAAGTATA ACTCAAATTC GGATAATGGC GAAATGAATA CAACGAACGA GGTCGATTTT 
GATTTCAATA CTAGTAAAAG GGCTTCTTTG TTACAATCCA TACCGGAAAG AGATCCCAAA 
AGAAATGTCT CAGATGCTAC CATTAAGCGA CGTGAATCCG ATGGCAATGG CAGGAGACTA 
AGTAATGTCA ACATATCCAT GAACCAGGAA AACATCAACA ATGATACTTT TCTATATAAA 
AAAAATAATC GCGATGGTCA TTTATCAGCA GTTTCTCATA TGTCCAGCTC TTCCCGTAGA 
TCGTTCATCT CCAATACGCT GCCGTTGAAT ATTGATTCCG CCTCAGAATC TGATAATTTT 
GTACCGCATA TGGATGGTTC ACCCTCCAAA ACAAAATCAG CCCCAGTTAG CTACGATAAG 
GATGGCATGA ATGCTTCAGA AGAAGACTTC AGTTTTGATA ATACTTTAGC CAAACCGTAT 
GAACCGTTAT ATGCTAGGAG AGGAGACATA ACTTCCGCCG GATCAACTAG TGGAGAAGAT 
AGCTCTCAGC CAAAGATGAT AACAATATCT GGTGAGCAAT TGAATTTGAT AACAGAAAAC 
AAAGAATTAA TGAATGAGTT GACACTGGTG TCTACAGAAT TAGCAGAATC GATAAAAAGG 
GAAACTGAAT TAGAAGAAAG AATACGGCTG TATGAAACCA ATAATTCAGC ACCATCTTTT 
GACGATTCTT CGAGTGTGTC TTTTTCTGAC TTTGAAAAGG AACTGAGAAA AAAATCCAGT 
AAAATTGTGC AATTGATCCA GCAATTAAAT GACGAGAGAT TAAAGAGATT CATTGCGGAG 
GAGCAGTTAT TACTCCAAGA AAATGGCACC AAACCAAGTT CTATGGAACT AGTAGGAAGA 
ATTGAAAACT TGAATAAACT TATTGATGAG AGAGATTCAG AAATTGAAAT GCTTAAAGGA 
CGTTTACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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EPO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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