YKU80 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YKU80
Entrez Gene ID	855132
Full Name	ATP-dependent DNA helicase YKU80
Synonyms	HDF2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182606.1	NP_013824.1	ATP-dependent DNA helicase YKU80

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YKU80	NP_013824.1
Eremothecium gossypii	AGOS_ABL030W	NP_982917.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the yKu-distal portion of telomeres is bound by Rap1, which in turn reduces the potential for yKu(Yku80 and Yku70 )to mediate NHEJ. These results thus propose a solution to a long-standing conundrum, namely how to accommodate the apparently conflicting functions of Ku on telomeres.
Title: Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.

The authors show that Sir4 - a previously identified Ku-binding protein that is a component of telomeric silent chromatin - is required for Ku-mediated telomere lengthening and telomerase recruitment.
Title: The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae.

Deletion of Est1 has a much greater impact on telomere lengthening in the presence of a Cdc13-Est2 fusion than deletion of YKU80, suggesting that any contribution that Ku may have in telomerase activation is minor compared to that of Est1.
Title: The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.

These findings contradict the recruitment model of Ku binding both DNA and RNA simultaneously and lead to a new model for the mechanism by which Ku's interaction with TLC1 RNA contributes to telomerase recruitment.
Title: Mutually exclusive binding of telomerase RNA and DNA by Ku alters telomerase recruitment model.

The TORC1 signal is transduced by the Gln3/Gat1/Ure2 pathway, which controls the levels of the Ku heterodimer, a telomere regulator.
Title: Tor complex 1 controls telomere length by affecting the level of Ku.

The following YKU80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YKU80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04102	NM_001182606.1 Latest version!	Saccharomyces cerevisiae S288C ATP-dependent DNA helicase YKU80 (YKU80), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YKU80 ( NM_001182606.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013824.1 Saccharomyces cerevisiae S288C ATP-dependent DNA helicase YKU80 (YKU80), partial mRNA.

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CloneID	OSi04102
Clone ID Related Accession (Same CDS sequence)	NM_001182606.1
Accession Version	NM_001182606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-dependent DNA helicase YKU80
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAAGTG AGTCAACAAC TTTCATCGTG GATGTTTCAC CATCAATGAT GAAAAATAAT 
AATGTTTCCA AATCTATGGC ATATCTAGAG TACACTCTAT TAAACAAATC TAAAAAAAGT 
AGAAAAACAG ATTGGATAAG CTGTTATCTC GCTAATTGTC CCGTATCTGA GAATTCCCAG 
GAAATACCAA ATGTGTTCCA AATACAATCC TTTTTGGCTC CTGTTACCAC GACGGCCACC 
ATTGGGTTTA TAAAACGCCT GAAACAATAT TGTGATCAGC ACAGTCATGA TAGTTCGAAT 
GAAGGCTTAC AATCAATGAT TCAATGTCTG TTGGTCGTCT CATTAGATAT TAAACAACAA 
TTTCAAGCAA GAAAAATTCT AAAACAAATT GTTGTATTTA CAGACAATTT AGATGATCTG 
GATATTACTG ATGAAGAAAT AGACCTGCTA ACGGAAGAAT TAAGCACAAG AATCATTTTG 
ATTGATTGCG GTAAAGACAC TCAAGAGGAG AGGAAAAAAT CGAACTGGTT AAAGTTGGTC 
GAAGCGATAC CGAATTCAAG AATCTACAAT ATGAATGAAC TTCTTGTTGA AATCACTTCC 
CCAGCAACAT CTGTCGTTAA GCCAGTAAGA GTATTCTCAG GCGAGCTTAG GTTAGGGGCA 
GATATATTAT CAACACAAAC ATCGAATCCT AGCGGCAGCA TGCAAGATGA AAATTGCTTA 
TGTATCAAGG TAGAGGCTTT TCCTGCTACG AAAGCAGTTT CAGGCTTAAA TAGGAAAACC 
GCTGTTGAGG TGGAAGACTC CCAGAAGAAA GAAAGGTATG TGGGGGTCAA ATCCATAATC 
GAATATGAAA TTCACAACGA AGGAAATAAG AAAAATGTCA GTGAAGATGA CCAGTCTGGA 
TCGTCATACA TTCCCGTGAC CATCTCCAAA GACTCTGTAA CGAAGGCATA CCGTTATGGT 
GCAGACTACG TAGTGCTACC GTCCGTTCTA GTCGATCAAA CTGTATATGA ATCTTTTCCA 
GGCTTGGATT TAAGAGGATT TTTGAATAGA GAAGCTCTTC CAAGGTACTT TTTGACGTCG 
GAATCCTCCT TCATTACCGC CGACACAAGA CTTGGCTGTC AATCTGACTT AATGGCTTTC 
AGTGCTCTAG TGGATGTTAT GCTAGAAAAT AGAAAAATTG CTGTTGCAAG ATATGTGAGC 
AAGAAAGACA GTGAAGTGAA TATGTGTGCA TTGTGTCCCG TTCTAATTGA GCACAGTAAC 
ATTAATTCCG AGAAGAAGTT TGTTAAGAGC CTTACCTTGT GTAGATTACC ATTCGCGGAA 
GACGAGAGGG TAACTGATTT TCCAAAACTT CTGGATCGAA CAACTACATC CGGGGTACCG 
CTGAAAAAGG AAACAGACGG GCATCAAATC GATGAGCTTA TGGAACAATT TGTAGATTCA 
ATGGATACAG ATGAGCTACC AGAGATTCCG TTAGGCAACT ACTATCAACC CATAGGCGAA 
GTAACAACAG ACACCACTCT CCCACTTCCC TCTTTAAATA AAGACCAAGA GGAAAACAAA 
AAGGATCCTC TTAGAATACC CACGGTTTTT GTCTACAGGC AACAACAGGT GCTACTGGAA 
TGGATTCACC AACTGATGAT CAATGATTCC AGGGAATTTG AAATCCCAGA GCTTCCTGAC 
TCATTGAAAA ACAAAATAAG TCCTTACACG CACAAGAAAT TTGATAGCAC AAAATTGGTT 
GAAGTGCTCG GCATCAAGAA AGTAGACAAA TTAAAACTCG ACTCCGAACT AAAAACTGAG 
CTTGAGAGGG AAAAGATCCC GGACCTAGAA ACGCTATTGA AGCGCGGTGA ACAACACAGT 
AGGGGAAGTC CAAACAATAG CAATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013824.1
CDS	1..1890
Translation	MSSESTTFIVDVSPSMMKNNNVSKSMAYLEYTLLNKSKKSRKTDWISCYLANCPVSENSQEIPNVFQIQSFLAPVTTTATIGFIKRLKQYCDQHSHDSSNEGLQSMIQCLLVVSLDIKQQFQARKILKQIVVFTDNLDDLDITDEEIDLLTEELSTRIILIDCGKDTQEERKKSNWLKLVEAIPNSRIYNMNELLVEITSPATSVVKPVRVFSGELRLGADILSTQTSNPSGSMQDENCLCIKVEAFPATKAVSGLNRKTAVEVEDSQKKERYVGVKSIIEYEIHNEGNKKNVSEDDQSGSSYIPVTISKDSVTKAYRYGADYVVLPSVLVDQTVYESFPGLDLRGFLNREALPRYFLTSESSFITADTRLGCQSDLMAFSALVDVMLENRKIAVARYVSKKDSEVNMCALCPVLIEHSNINSEKKFVKSLTLCRLPFAEDERVTDFPKLLDRTTTSGVPLKKETDGHQIDELMEQFVDSMDTDELPEIPLGNYYQPIGEVTTDTTLPLPSLNKDQEENKKDPLRIPTVFVYRQQQVLLEWIHQLMINDSREFEIPELPDSLKNKISPYTHKKFDSTKLVEVLGIKKVDKLKLDSELKTELEREKIPDLETLLKRGEQHSRGSPNNSNN

Target ORF information:

RefSeq Version	NM_001182606.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-dependent DNA helicase YKU80 (YKU80), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182606.1

ORF Insert Sequence:

ATGTCAAGTG AGTCAACAAC TTTCATCGTG GATGTTTCAC CATCAATGAT GAAAAATAAT 
AATGTTTCCA AATCTATGGC ATATCTAGAG TACACTCTAT TAAACAAATC TAAAAAAAGT 
AGAAAAACAG ATTGGATAAG CTGTTATCTC GCTAATTGTC CCGTATCTGA GAATTCCCAG 
GAAATACCAA ATGTGTTCCA AATACAATCC TTTTTGGCTC CTGTTACCAC GACGGCCACC 
ATTGGGTTTA TAAAACGCCT GAAACAATAT TGTGATCAGC ACAGTCATGA TAGTTCGAAT 
GAAGGCTTAC AATCAATGAT TCAATGTCTG TTGGTCGTCT CATTAGATAT TAAACAACAA 
TTTCAAGCAA GAAAAATTCT AAAACAAATT GTTGTATTTA CAGACAATTT AGATGATCTG 
GATATTACTG ATGAAGAAAT AGACCTGCTA ACGGAAGAAT TAAGCACAAG AATCATTTTG 
ATTGATTGCG GTAAAGACAC TCAAGAGGAG AGGAAAAAAT CGAACTGGTT AAAGTTGGTC 
GAAGCGATAC CGAATTCAAG AATCTACAAT ATGAATGAAC TTCTTGTTGA AATCACTTCC 
CCAGCAACAT CTGTCGTTAA GCCAGTAAGA GTATTCTCAG GCGAGCTTAG GTTAGGGGCA 
GATATATTAT CAACACAAAC ATCGAATCCT AGCGGCAGCA TGCAAGATGA AAATTGCTTA 
TGTATCAAGG TAGAGGCTTT TCCTGCTACG AAAGCAGTTT CAGGCTTAAA TAGGAAAACC 
GCTGTTGAGG TGGAAGACTC CCAGAAGAAA GAAAGGTATG TGGGGGTCAA ATCCATAATC 
GAATATGAAA TTCACAACGA AGGAAATAAG AAAAATGTCA GTGAAGATGA CCAGTCTGGA 
TCGTCATACA TTCCCGTGAC CATCTCCAAA GACTCTGTAA CGAAGGCATA CCGTTATGGT 
GCAGACTACG TAGTGCTACC GTCCGTTCTA GTCGATCAAA CTGTATATGA ATCTTTTCCA 
GGCTTGGATT TAAGAGGATT TTTGAATAGA GAAGCTCTTC CAAGGTACTT TTTGACGTCG 
GAATCCTCCT TCATTACCGC CGACACAAGA CTTGGCTGTC AATCTGACTT AATGGCTTTC 
AGTGCTCTAG TGGATGTTAT GCTAGAAAAT AGAAAAATTG CTGTTGCAAG ATATGTGAGC 
AAGAAAGACA GTGAAGTGAA TATGTGTGCA TTGTGTCCCG TTCTAATTGA GCACAGTAAC 
ATTAATTCCG AGAAGAAGTT TGTTAAGAGC CTTACCTTGT GTAGATTACC ATTCGCGGAA 
GACGAGAGGG TAACTGATTT TCCAAAACTT CTGGATCGAA CAACTACATC CGGGGTACCG 
CTGAAAAAGG AAACAGACGG GCATCAAATC GATGAGCTTA TGGAACAATT TGTAGATTCA 
ATGGATACAG ATGAGCTACC AGAGATTCCG TTAGGCAACT ACTATCAACC CATAGGCGAA 
GTAACAACAG ACACCACTCT CCCACTTCCC TCTTTAAATA AAGACCAAGA GGAAAACAAA 
AAGGATCCTC TTAGAATACC CACGGTTTTT GTCTACAGGC AACAACAGGT GCTACTGGAA 
TGGATTCACC AACTGATGAT CAATGATTCC AGGGAATTTG AAATCCCAGA GCTTCCTGAC 
TCATTGAAAA ACAAAATAAG TCCTTACACG CACAAGAAAT TTGATAGCAC AAAATTGGTT 
GAAGTGCTCG GCATCAAGAA AGTAGACAAA TTAAAACTCG ACTCCGAACT AAAAACTGAG 
CTTGAGAGGG AAAAGATCCC GGACCTAGAA ACGCTATTGA AGCGCGGTGA ACAACACAGT 
AGGGGAAGTC CAAACAATAG CAATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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YKU80 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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