FET3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FET3
Entrez Gene ID	855080
Full Name	ferroxidase FET3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182556.1	NP_013774.1	ferroxidase FET3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	FET3	NP_013774.1
Kluyveromyces lactis	KLLA0F26400g	XP_456256.1
Schizosaccharomyces pombe (fission yeast)	fio1	NP_594494.1
Magnaporthe oryzae (rice blast fungus)	MGG_02156	XP_003708928.1
Neurospora crassa	NCU03498	XP_955835.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study reveals a novel functional role of K(+) in the binding of copper to apoFet3p and identifies a K(+)/H(+) exchanger at the secretory pathway as a new molecular factor associated with iron uptake in yeast.
Title: Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase.

Presence of crowding agents does not affect Fet3p structural content but increases thermal resistance. The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics.
Title: Non-linear effects of macromolecular crowding on enzymatic activity of multi-copper oxidase.

We find that unfolding reactions of apo and different holo forms of Fet3p are irreversible reactions that depend on the scan rate and there is a metal-induced tradeoff between overall protein stability and metal coordination
Title: In vitro unfolding of yeast multicopper oxidase Fet3p variants reveals unique role of each metal site.

Data show that overproduction of Izh2p prevents the iron-dependent induction of the Fet3p component of the high-affinity iron-uptake system and is deleterious for growth in iron-limited medium.
Title: Probing the mechanism of FET3 repression by Izh2p overexpression.

Structure-function analysis of the cuprous oxidase activity in Fet3p from Saccharomyces cerevisiae
Title: Structure-function analysis of the cuprous oxidase activity in Fet3p from Saccharomyces cerevisiae.

The following FET3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FET3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04056	NM_001182556.1 Latest version!	Saccharomyces cerevisiae S288C ferroxidase FET3 (FET3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FET3 ( NM_001182556.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013774.1 Saccharomyces cerevisiae S288C ferroxidase FET3 (FET3), partial mRNA.

Price & Availability↑

CloneID	OSi04056
Clone ID Related Accession (Same CDS sequence)	NM_001182556.1
Accession Version	NM_001182556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ferroxidase FET3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTAACG CTTTGCTCTC TATAGCCGTT TTGCTTTTCT CGATGCTCTC GCTAGCACAA 
GCGGAGACGC ACACGTTTAA TTGGACCACT GGCTGGGACT ACAGGAACGT TGATGGGCTA 
AAGAGCCGTC CCGTGATCAC CTGTAATGGC CAGTTCCCAT GGCCAGATAT AACGGTCAAC 
AAAGGTGACC GTGTGCAGAT TTACTTGACC AACGGAATGA ACAACACCAA TACTTCTATG 
CATTTCCACG GTCTCTTCCA AAACGGAACC GCCTCTATGG ACGGTGTGCC CTTCTTGACG 
CAATGTCCAA TTGCGCCAGG CAGTACTATG CTTTACAATT TCACGGTGGA CTACAATGTA 
GGCACCTACT GGTACCATTC ACACACGGAC GGTCAATATG AAGACGGGAT GAAAGGTCTT 
TTCATCATCA AGGATGATAG CTTCCCCTAC GATTACGATG AGGAACTTTC TTTATCGCTT 
AGTGAGTGGT ACCACGACTT GGTCACGGAC TTGACGAAGT CGTTCATGAG TGTTTATAAT 
CCGACAGGTG CTGAGCCCAT CCCACAGAAC TTGATTGTTA ACAACACGAT GAATCTGACA 
TGGGAAGTCC AGCCCGATAC GACGTATCTT TTGAGAATTG TCAACGTGGG TGGGTTCGTT 
TCGCAGTACT TTTGGATCGA GGACCACGAA ATGACCGTGG TCGAAATCGA CGGTATCACT 
ACCGAGAAGA ACGTAACGGA TATGCTTTAC ATCACTGTCG CTCAGAGATA TACAGTCCTG 
GTTCACACTA AAAACGACAC GGACAAAAAT TTCGCCATCA TGCAGAAATT TGATGACACC 
ATGTTGGATG TCATTCCAAG TGATTTACAG CTGAATGCAA CCTCTTATAT GGTCTACAAC 
AAAACCGCTG CGCTGCCCAC ACAAAATTAC GTGGATTCAA TTGATAACTT CTTGGACGAT 
TTCTACTTGC AACCGTACGA GAAAGAAGCC ATCTATGGCG AGCCAGATCA TGTGATTACC 
GTTGACGTTG TTATGGATAA CTTGAAAAAC GGTGTGAATT ACGCCTTCTT CAATAATATC 
ACCTATACTG CACCAAAAGT TCCTACTTTG ATGACCGTTT TGTCTTCAGG TGATCAAGCA 
AACAACTCCG AAATCTACGG TTCAAACACG CACACTTTCA TCCTAGAGAA GGATGAAATC 
GTGGAGATTG TGCTAAATAA CCAGGACACA GGTACCCATC CTTTCCATTT ACATGGTCAC 
GCTTTCCAAA CCATCCAGAG AGATCGTACA TATGATGATG CCCTAGGTGA AGTTCCTCAC 
AGTTTCGATC CGGACAACCA CCCTGCCTTC CCAGAATACC CAATGAGAAG AGATACTTTA 
TACGTTAGAC CACAATCCAA TTTCGTCATC AGGTTTAAAG CCGATAACCC AGGTGTTTGG 
TTCTTCCATT GTCATATCGA ATGGCATTTG TTGCAAGGTT TGGGTCTTGT TCTCGTGGAG 
GATCCTTTTG GTATCCAAGA TGCTCATTCT CAACAACTCA GTGAAAACCA CTTAGAAGTT 
TGCCAGAGTT GCTCTGTGGC CACTGAAGGT AACGCCGCTG CCAATACACT GGATTTAACT 
GATTTAACTG GTGAAAATGT TCAGCATGCC TTCATTCCTA CCGGTTTTAC CAAAAAAGGT 
ATTATTGCCA TGACATTCTC CTGCTTTGCC GGTATTCTTG GTATTATCAC AATTGCAATT 
TATGGTATGA TGGATATGGA AGATGCGACC GAAAAGGTTA TTCGAGACTT GCACGTGGAC 
CCTGAAGTCT TGCTAAATGA GGTTGATGAA AATGAAGAGC GTCAGGTAAA CGAAGATCGT 
CATTCCACTG AAAAGCATCA ATTTTTAACT AAAGCCAAAC GGTTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013774.1
CDS	1..1911
Translation	MTNALLSIAVLLFSMLSLAQAETHTFNWTTGWDYRNVDGLKSRPVITCNGQFPWPDITVNKGDRVQIYLTNGMNNTNTSMHFHGLFQNGTASMDGVPFLTQCPIAPGSTMLYNFTVDYNVGTYWYHSHTDGQYEDGMKGLFIIKDDSFPYDYDEELSLSLSEWYHDLVTDLTKSFMSVYNPTGAEPIPQNLIVNNTMNLTWEVQPDTTYLLRIVNVGGFVSQYFWIEDHEMTVVEIDGITTEKNVTDMLYITVAQRYTVLVHTKNDTDKNFAIMQKFDDTMLDVIPSDLQLNATSYMVYNKTAALPTQNYVDSIDNFLDDFYLQPYEKEAIYGEPDHVITVDVVMDNLKNGVNYAFFNNITYTAPKVPTLMTVLSSGDQANNSEIYGSNTHTFILEKDEIVEIVLNNQDTGTHPFHLHGHAFQTIQRDRTYDDALGEVPHSFDPDNHPAFPEYPMRRDTLYVRPQSNFVIRFKADNPGVWFFHCHIEWHLLQGLGLVLVEDPFGIQDAHSQQLSENHLEVCQSCSVATEGNAAANTLDLTDLTGENVQHAFIPTGFTKKGIIAMTFSCFAGILGIITIAIYGMMDMEDATEKVIRDLHVDPEVLLNEVDENEERQVNEDRHSTEKHQFLTKAKRFF

Target ORF information:

RefSeq Version	NM_001182556.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ferroxidase FET3 (FET3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182556.1

ORF Insert Sequence:

ATGACTAACG CTTTGCTCTC TATAGCCGTT TTGCTTTTCT CGATGCTCTC GCTAGCACAA 
GCGGAGACGC ACACGTTTAA TTGGACCACT GGCTGGGACT ACAGGAACGT TGATGGGCTA 
AAGAGCCGTC CCGTGATCAC CTGTAATGGC CAGTTCCCAT GGCCAGATAT AACGGTCAAC 
AAAGGTGACC GTGTGCAGAT TTACTTGACC AACGGAATGA ACAACACCAA TACTTCTATG 
CATTTCCACG GTCTCTTCCA AAACGGAACC GCCTCTATGG ACGGTGTGCC CTTCTTGACG 
CAATGTCCAA TTGCGCCAGG CAGTACTATG CTTTACAATT TCACGGTGGA CTACAATGTA 
GGCACCTACT GGTACCATTC ACACACGGAC GGTCAATATG AAGACGGGAT GAAAGGTCTT 
TTCATCATCA AGGATGATAG CTTCCCCTAC GATTACGATG AGGAACTTTC TTTATCGCTT 
AGTGAGTGGT ACCACGACTT GGTCACGGAC TTGACGAAGT CGTTCATGAG TGTTTATAAT 
CCGACAGGTG CTGAGCCCAT CCCACAGAAC TTGATTGTTA ACAACACGAT GAATCTGACA 
TGGGAAGTCC AGCCCGATAC GACGTATCTT TTGAGAATTG TCAACGTGGG TGGGTTCGTT 
TCGCAGTACT TTTGGATCGA GGACCACGAA ATGACCGTGG TCGAAATCGA CGGTATCACT 
ACCGAGAAGA ACGTAACGGA TATGCTTTAC ATCACTGTCG CTCAGAGATA TACAGTCCTG 
GTTCACACTA AAAACGACAC GGACAAAAAT TTCGCCATCA TGCAGAAATT TGATGACACC 
ATGTTGGATG TCATTCCAAG TGATTTACAG CTGAATGCAA CCTCTTATAT GGTCTACAAC 
AAAACCGCTG CGCTGCCCAC ACAAAATTAC GTGGATTCAA TTGATAACTT CTTGGACGAT 
TTCTACTTGC AACCGTACGA GAAAGAAGCC ATCTATGGCG AGCCAGATCA TGTGATTACC 
GTTGACGTTG TTATGGATAA CTTGAAAAAC GGTGTGAATT ACGCCTTCTT CAATAATATC 
ACCTATACTG CACCAAAAGT TCCTACTTTG ATGACCGTTT TGTCTTCAGG TGATCAAGCA 
AACAACTCCG AAATCTACGG TTCAAACACG CACACTTTCA TCCTAGAGAA GGATGAAATC 
GTGGAGATTG TGCTAAATAA CCAGGACACA GGTACCCATC CTTTCCATTT ACATGGTCAC 
GCTTTCCAAA CCATCCAGAG AGATCGTACA TATGATGATG CCCTAGGTGA AGTTCCTCAC 
AGTTTCGATC CGGACAACCA CCCTGCCTTC CCAGAATACC CAATGAGAAG AGATACTTTA 
TACGTTAGAC CACAATCCAA TTTCGTCATC AGGTTTAAAG CCGATAACCC AGGTGTTTGG 
TTCTTCCATT GTCATATCGA ATGGCATTTG TTGCAAGGTT TGGGTCTTGT TCTCGTGGAG 
GATCCTTTTG GTATCCAAGA TGCTCATTCT CAACAACTCA GTGAAAACCA CTTAGAAGTT 
TGCCAGAGTT GCTCTGTGGC CACTGAAGGT AACGCCGCTG CCAATACACT GGATTTAACT 
GATTTAACTG GTGAAAATGT TCAGCATGCC TTCATTCCTA CCGGTTTTAC CAAAAAAGGT 
ATTATTGCCA TGACATTCTC CTGCTTTGCC GGTATTCTTG GTATTATCAC AATTGCAATT 
TATGGTATGA TGGATATGGA AGATGCGACC GAAAAGGTTA TTCGAGACTT GCACGTGGAC 
CCTGAAGTCT TGCTAAATGA GGTTGATGAA AATGAAGAGC GTCAGGTAAA CGAAGATCGT 
CATTCCACTG AAAAGCATCA ATTTTTAACT AAAGCCAAAC GGTTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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FET3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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