BUB2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB2
Entrez Gene ID	855077
Full Name	Bub2p
Synonyms	PAC7
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182553.1	NP_013771.1	Bub2p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F26422g	XP_456257.1
Magnaporthe oryzae (rice blast fungus)	MGG_04676	XP_003710862.1
Saccharomyces cerevisiae (baker's yeast)	BUB2	NP_013771.1
Eremothecium gossypii	AGOS_ADR131C	NP_984227.1
Schizosaccharomyces pombe (fission yeast)	cdc16	NP_593901.1
Neurospora crassa	NCU03237	XP_965337.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that spindle checkpoint factors Bub1 and Bub2 are recruited to the site of DNA damage.
Title: Spindle Checkpoint Factors Bub1 and Bub2 Promote DNA Double-Strand Break Repair by Nonhomologous End Joining.

regulation of cytokinesis and septation in budding yeast by Bub2.
Title: Bub2 regulation of cytokinesis and septation in budding yeast.

there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles
Title: Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations.

Bub2 disappearance from the mother bound spindle pole body at the onset of anaphase, along with Cdc5 function, helps prompt Tem1 activation in telophase.
Title: Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit.

The following BUB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04054	NM_001182553.1 Latest version!	Saccharomyces cerevisiae S288C Bub2p (BUB2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB2 ( NM_001182553.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013771.1 Saccharomyces cerevisiae S288C Bub2p (BUB2), partial mRNA.

Price & Availability↑

CloneID	OSi04054
Clone ID Related Accession (Same CDS sequence)	NM_001182553.1
Accession Version	NM_001182553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bub2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACCTCAA TTGAAGATCT GATATCAAAT CCTCCCCTGC TACTGCATTC CTCACTCTCT 
CAACTTCGCT ACCTCATACT CAGTGAAGGG CTTCCCATAT CTGAAGATAA ACAGCAGCAG 
CGCACGCGAT GTTACGTGTG GACGGTGCTC TCTCAAACTT CTATGGAGGC GTCTACACAA 
CGGTACCTTG CGCTCTTGAA ACTGGGCCCA CCTTCCACAA CCATTTACCA AAAAATCAAG 
AACGACACAT CCAGAACTTT TCAGACGGAT CCAAACTTTA GAAATAGAGT CTCAGAGGAT 
GCCCTCATCC GGTGTCTGTC GTGCTTTGCT TGGCAAACAC AACAAAGAAG ACAAAAGACT 
CGTTTTGGTC GCATTCCTGT GAGCACATAT GTGCAGGGCA TGAACGTGTT ATTAGCTCCT 
CTGCTTTACT CATGTCCTTC TGAACCTATG GCGTATCAAC TTTTCACTAA GCTTTGTTAT 
GAAATGATAC CGACGTATCT AACTAAGAAC CTAAACGGCG CCCAGAACGG CGCCAAACTA 
CTAGATATTT CTCTCAGAAT CATTGATCCA AAGCTGAGTA AATTTCTATC GGACAATTTA 
CTTACAGCAG AGATTTATGG TATGCCTTCC ATACTCACGT TATCCAGCTG CAATAAACCG 
CTTGATCAGG TCATTAAACT GTGGGATTTC ATGTTTGCAT ATGGATTCCA TATGAATATT 
CTCTTTGTGG TGGCATTCCT AGTTAAAATG AGATCCAAGG TTTTCAAATC GGATTCCCCT 
GTTAATTTAC TACGACAGTT TCCGGATTTT GATGCTGACG AGATCATTCG GCTGGGCGTC 
GGGTTCATCG CTAAGATCCC TGCTCAAATT TATGACCTAT TGGTAGACCA CTTGACCGAC 
CCAGACATAT ATATACCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013771.1
CDS	1..921
Translation	MTSIEDLISNPPLLLHSSLSQLRYLILSEGLPISEDKQQQRTRCYVWTVLSQTSMEASTQRYLALLKLGPPSTTIYQKIKNDTSRTFQTDPNFRNRVSEDALIRCLSCFAWQTQQRRQKTRFGRIPVSTYVQGMNVLLAPLLYSCPSEPMAYQLFTKLCYEMIPTYLTKNLNGAQNGAKLLDISLRIIDPKLSKFLSDNLLTAEIYGMPSILTLSSCNKPLDQVIKLWDFMFAYGFHMNILFVVAFLVKMRSKVFKSDSPVNLLRQFPDFDADEIIRLGVGFIAKIPAQIYDLLVDHLTDPDIYIP

Target ORF information:

RefSeq Version	NM_001182553.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bub2p (BUB2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182553.1

ORF Insert Sequence:

ATGACCTCAA TTGAAGATCT GATATCAAAT CCTCCCCTGC TACTGCATTC CTCACTCTCT 
CAACTTCGCT ACCTCATACT CAGTGAAGGG CTTCCCATAT CTGAAGATAA ACAGCAGCAG 
CGCACGCGAT GTTACGTGTG GACGGTGCTC TCTCAAACTT CTATGGAGGC GTCTACACAA 
CGGTACCTTG CGCTCTTGAA ACTGGGCCCA CCTTCCACAA CCATTTACCA AAAAATCAAG 
AACGACACAT CCAGAACTTT TCAGACGGAT CCAAACTTTA GAAATAGAGT CTCAGAGGAT 
GCCCTCATCC GGTGTCTGTC GTGCTTTGCT TGGCAAACAC AACAAAGAAG ACAAAAGACT 
CGTTTTGGTC GCATTCCTGT GAGCACATAT GTGCAGGGCA TGAACGTGTT ATTAGCTCCT 
CTGCTTTACT CATGTCCTTC TGAACCTATG GCGTATCAAC TTTTCACTAA GCTTTGTTAT 
GAAATGATAC CGACGTATCT AACTAAGAAC CTAAACGGCG CCCAGAACGG CGCCAAACTA 
CTAGATATTT CTCTCAGAAT CATTGATCCA AAGCTGAGTA AATTTCTATC GGACAATTTA 
CTTACAGCAG AGATTTATGG TATGCCTTCC ATACTCACGT TATCCAGCTG CAATAAACCG 
CTTGATCAGG TCATTAAACT GTGGGATTTC ATGTTTGCAT ATGGATTCCA TATGAATATT 
CTCTTTGTGG TGGCATTCCT AGTTAAAATG AGATCCAAGG TTTTCAAATC GGATTCCCCT 
GTTAATTTAC TACGACAGTT TCCGGATTTT GATGCTGACG AGATCATTCG GCTGGGCGTC 
GGGTTCATCG CTAAGATCCC TGCTCAAATT TATGACCTAT TGGTAGACCA CTTGACCGAC 
CCAGACATAT ATATACCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BUB2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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