NDC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NDC1
Entrez Gene ID	854977
Full Name	Ndc1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182389.1	NP_013681.1	Ndc1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B01760g	XP_451609.1
Saccharomyces cerevisiae (baker's yeast)	NDC1	NP_013681.1
Eremothecium gossypii	AGOS_AER307W	NP_985163.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mps2-Bbp1 directly interacts with Mps3 and Ndc1. We then purified Spc29 and reconstituted the ternary Mps2-Bbp1-Spc29 complex, proving that Bbp1 can simultaneously interact with Mps2 and Spc29 and in this way link the central plaque of the spindle pole body to the nuclear envelope
Title: A ternary membrane protein complex anchors the spindle pole body in the nuclear envelope in budding yeast.

overexpression of NDC1, which is also required for nuclear pore complexes biogenesis, rescued both the spindle pole bodies and spindle pole bodies associated defects.
Title: Integrity and function of the Saccharomyces cerevisiae spindle pole body depends on connections between the membrane proteins Ndc1, Rtn1, and Yop1.

Ndc1 together with its interaction partners are collectively essential for the biosynthesis and structural integrity of yeast nuclear pore complex.
Title: Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance.

The absence of Ndc1p results in aberrant nuclear pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus.
Title: The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function.

Nbp1p binds to Ndc1p
Title: The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.

The following NDC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03969	NM_001182389.1 Latest version!	Saccharomyces cerevisiae S288C Ndc1p (NDC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDC1 ( NM_001182389.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013681.1 Saccharomyces cerevisiae S288C Ndc1p (NDC1), partial mRNA.

Price & Availability↑

CloneID	OSi03969
Clone ID Related Accession (Same CDS sequence)	NM_001182389.1
Accession Version	NM_001182389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ndc1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATACAGA CGCCAAGGGA GTTGTTGAAT CCACGGTACA CGTACCATAC CATCTTTAGC 
GACGTGTGCA AGACCCGATT CAACCATTTA GTTACGAGGT TGTTTTTCAT ATGTTCTATT 
ATCCAAACTG TGGTGATTTC ACTGCTGGCG TTGCCACACT CACCGTTATG GGAACTGGCA 
TTGGCATTTA TTCCCAATAT TTTAGCTCTT AACCTAGTTT CCCTTTTGAT CATTGTGACA 
AGAAAAAATT ACATGCATGT AAAGAATTTT GGGTTCGCTA ATTCACTTAC TTTCATTCTA 
GGCCAATTGT TATCAGTAAA GTTTCTTGTA TACCAGGGAG TCTATAGCAT GGGCAGCATC 
CTTTTATCAT TCGTACTGGG TGTGGTTTTC GGACGTGGCG GATCTGGATG GAAGCCATAC 
TATAAGCTGT TTATTTGGCT AGTCGTTCCC ACGATTTACA ACCTACAACA TCATGTCACT 
GACGCTGACA AATTGTCGTT CAACTGCGAA AACTTTTTCC AGGCCCCACA AGACTACGTA 
TTAGAGCGTG TCAAGAGGAT TATGGAGAAG TCAGTCATTC TAAGTGTAAT CAGCATGTTC 
GTATTACCAA TCTTTACCAC AGTATTTTTT TCCAGGCAAA AATCGGGATT ATTTGATTCA 
TTTACGAATG GCGTGCTAGC TGTCACAAAT TTACTAATAA TCTCCTGCAT TATCTTCATC 
ACTTTCGAAT TCATAAATAT AGCATTTGAC GCACATATGT CCATAGGTTG TCTGCACAAG 
GGCAAGCTAA TTTCGAATTT GTCGTCGACG CCGATGGAAA CCCTGCTTAG TGGGCTTTCT 
GCAGACAAGC CATTTACTAG GTTGACTGCC TACCAGGAAC TAGCGTATAG GGCCACTTCA 
CTGGATCCAT CATTGCGAGC ACCAATATAT CACTCCAAGT TCAGAAGCAG TAGTGGTAAT 
ACTTGGTCAT TAATCCTCAA TGAATGCTTG AAGACCATTC AGATAAACAA TGAAAAGGTT 
GTGCAGTACC TAAGATCAGT ACAAGATCTT GGGGGCTCTG CAACTGCTAG ACATAAAAAA 
AAAGTAGAAA ATCTAGATTA TATGTATGAG AACGGGAAAC TAACAAGCGC CAACGAAAGA 
CTCTTTGGGA ACCGACCCTC TATGATGGCG CCATTACGGG ATAACGGCTT ATTAGATGAA 
TCACCAAACA GGCTGAGGGT AAGAACTGAC GATAGTGTAT TGCTTAACCG CGGAAACAAG 
AAAAGGCATA GGTCCTCGTA TTACGACAAC GATTTAGATG AAACGACACA AACTTTTAAC 
GGCTCTATTT TCACCCACGA AACAACATTT ATGACCGCTA TGCGTCTGAT GTTAAAAAAA 
CTGAAAAATT CTATAATGTC GTTTATTTTC CCTTCCTACG CAGAAAGACA ATCATCTGAT 
GAAAGCGACA ATTACAGACT ATTACCAAAT GGCTCCAACA AAGCCCAAAT CTCGATAATT 
GACATATGGT CCATTTCCAA GAAAAGGCAA GCTGAGAAGC TGGTTCCATT GCCCATATGC 
CACGCAAATA GTGTGGTAGC ACTGACAGGA CTGCTGATCC GATCAAAGAC AGAAGATCCA 
AAAGGCGGTA TCATTGCATC CGTTGGGGAT ATTCTTAAGA CTTTGGAGAG GTCCATCTGT 
GCACTGGGTG AATTTGCCGA TTGGGATCCG GAATCGATGG CATACACTGC CTTCCAAACA 
CAAAGGACAG CCCAAGATAG AGTACAACAA GACAGTGAAG ACGAAGATAG CATGAAAGAC 
ACTACTGACA TGATCAGTGT TCTTTATCAG CTGTCCACCA GTGCTTTTAT GGAGATTGTA 
CTTGAATACA ATGTTGCTCT GAACGACGTA TATTTGGATG CAGACGTGGC CAAGTTGGCG 
AACTGGTTTC TAGAAGTGTA CGCCTCAGGC AACCCTAATG CTACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013681.1
CDS	1..1968
Translation	MIQTPRELLNPRYTYHTIFSDVCKTRFNHLVTRLFFICSIIQTVVISLLALPHSPLWELALAFIPNILALNLVSLLIIVTRKNYMHVKNFGFANSLTFILGQLLSVKFLVYQGVYSMGSILLSFVLGVVFGRGGSGWKPYYKLFIWLVVPTIYNLQHHVTDADKLSFNCENFFQAPQDYVLERVKRIMEKSVILSVISMFVLPIFTTVFFSRQKSGLFDSFTNGVLAVTNLLIISCIIFITFEFINIAFDAHMSIGCLHKGKLISNLSSTPMETLLSGLSADKPFTRLTAYQELAYRATSLDPSLRAPIYHSKFRSSSGNTWSLILNECLKTIQINNEKVVQYLRSVQDLGGSATARHKKKVENLDYMYENGKLTSANERLFGNRPSMMAPLRDNGLLDESPNRLRVRTDDSVLLNRGNKKRHRSSYYDNDLDETTQTFNGSIFTHETTFMTAMRLMLKKLKNSIMSFIFPSYAERQSSDESDNYRLLPNGSNKAQISIIDIWSISKKRQAEKLVPLPICHANSVVALTGLLIRSKTEDPKGGIIASVGDILKTLERSICALGEFADWDPESMAYTAFQTQRTAQDRVQQDSEDEDSMKDTTDMISVLYQLSTSAFMEIVLEYNVALNDVYLDADVAKLANWFLEVYASGNPNAT

Target ORF information:

RefSeq Version	NM_001182389.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ndc1p (NDC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182389.1

ORF Insert Sequence:

ATGATACAGA CGCCAAGGGA GTTGTTGAAT CCACGGTACA CGTACCATAC CATCTTTAGC 
GACGTGTGCA AGACCCGATT CAACCATTTA GTTACGAGGT TGTTTTTCAT ATGTTCTATT 
ATCCAAACTG TGGTGATTTC ACTGCTGGCG TTGCCACACT CACCGTTATG GGAACTGGCA 
TTGGCATTTA TTCCCAATAT TTTAGCTCTT AACCTAGTTT CCCTTTTGAT CATTGTGACA 
AGAAAAAATT ACATGCATGT AAAGAATTTT GGGTTCGCTA ATTCACTTAC TTTCATTCTA 
GGCCAATTGT TATCAGTAAA GTTTCTTGTA TACCAGGGAG TCTATAGCAT GGGCAGCATC 
CTTTTATCAT TCGTACTGGG TGTGGTTTTC GGACGTGGCG GATCTGGATG GAAGCCATAC 
TATAAGCTGT TTATTTGGCT AGTCGTTCCC ACGATTTACA ACCTACAACA TCATGTCACT 
GACGCTGACA AATTGTCGTT CAACTGCGAA AACTTTTTCC AGGCCCCACA AGACTACGTA 
TTAGAGCGTG TCAAGAGGAT TATGGAGAAG TCAGTCATTC TAAGTGTAAT CAGCATGTTC 
GTATTACCAA TCTTTACCAC AGTATTTTTT TCCAGGCAAA AATCGGGATT ATTTGATTCA 
TTTACGAATG GCGTGCTAGC TGTCACAAAT TTACTAATAA TCTCCTGCAT TATCTTCATC 
ACTTTCGAAT TCATAAATAT AGCATTTGAC GCACATATGT CCATAGGTTG TCTGCACAAG 
GGCAAGCTAA TTTCGAATTT GTCGTCGACG CCGATGGAAA CCCTGCTTAG TGGGCTTTCT 
GCAGACAAGC CATTTACTAG GTTGACTGCC TACCAGGAAC TAGCGTATAG GGCCACTTCA 
CTGGATCCAT CATTGCGAGC ACCAATATAT CACTCCAAGT TCAGAAGCAG TAGTGGTAAT 
ACTTGGTCAT TAATCCTCAA TGAATGCTTG AAGACCATTC AGATAAACAA TGAAAAGGTT 
GTGCAGTACC TAAGATCAGT ACAAGATCTT GGGGGCTCTG CAACTGCTAG ACATAAAAAA 
AAAGTAGAAA ATCTAGATTA TATGTATGAG AACGGGAAAC TAACAAGCGC CAACGAAAGA 
CTCTTTGGGA ACCGACCCTC TATGATGGCG CCATTACGGG ATAACGGCTT ATTAGATGAA 
TCACCAAACA GGCTGAGGGT AAGAACTGAC GATAGTGTAT TGCTTAACCG CGGAAACAAG 
AAAAGGCATA GGTCCTCGTA TTACGACAAC GATTTAGATG AAACGACACA AACTTTTAAC 
GGCTCTATTT TCACCCACGA AACAACATTT ATGACCGCTA TGCGTCTGAT GTTAAAAAAA 
CTGAAAAATT CTATAATGTC GTTTATTTTC CCTTCCTACG CAGAAAGACA ATCATCTGAT 
GAAAGCGACA ATTACAGACT ATTACCAAAT GGCTCCAACA AAGCCCAAAT CTCGATAATT 
GACATATGGT CCATTTCCAA GAAAAGGCAA GCTGAGAAGC TGGTTCCATT GCCCATATGC 
CACGCAAATA GTGTGGTAGC ACTGACAGGA CTGCTGATCC GATCAAAGAC AGAAGATCCA 
AAAGGCGGTA TCATTGCATC CGTTGGGGAT ATTCTTAAGA CTTTGGAGAG GTCCATCTGT 
GCACTGGGTG AATTTGCCGA TTGGGATCCG GAATCGATGG CATACACTGC CTTCCAAACA 
CAAAGGACAG CCCAAGATAG AGTACAACAA GACAGTGAAG ACGAAGATAG CATGAAAGAC 
ACTACTGACA TGATCAGTGT TCTTTATCAG CTGTCCACCA GTGCTTTTAT GGAGATTGTA 
CTTGAATACA ATGTTGCTCT GAACGACGTA TATTTGGATG CAGACGTGGC CAAGTTGGCG 
AACTGGTTTC TAGAAGTGTA CGCCTCAGGC AACCCTAATG CTACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NDC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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