NTE1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NTE1
Entrez Gene ID	854943
Full Name	lysophospholipase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182418.1	NP_013652.1	lysophospholipase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	NTE1	NP_013652.1
Eremothecium gossypii	AGOS_AER124W	NP_984983.1
Neurospora crassa	NCU08276	XP_961904.2
Kluyveromyces lactis	KLLA0B05225g	XP_451767.1
Schizosaccharomyces pombe (fission yeast)	nte1	NP_588086.1
Magnaporthe oryzae (rice blast fungus)	MGG_06143	XP_003712018.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nte1p activity, by controlling PC abundance at the ER, affects lateral membrane packing and that this parameter, in turn, impacts the repressing transcriptional activity of Opi1p, the main regulator of phospholipid synthesis gene transcription.
Title: NTE1-encoded phosphatidylcholine phospholipase b regulates transcription of phospholipid biosynthetic genes.

Sec14p acts as a positive regulator of Nte1p-mediated PC deacylation with the functional consequence of increased Nte1p activity increasing the permissive temperature for the growth of sec14(ts) cells
Title: Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p.

The following NTE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NTE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03942	NM_001182418.1 Latest version!	Saccharomyces cerevisiae S288C lysophospholipase (NTE1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NTE1 ( NM_001182418.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013652.1 Saccharomyces cerevisiae S288C lysophospholipase (NTE1), partial mRNA.

Price & Availability↑

CloneID	OSi03942
Clone ID Related Accession (Same CDS sequence)	NM_001182418.1
Accession Version	NM_001182418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	lysophospholipase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCGTTCAA TGAATTGCAC TACGAACAAC ACCAACAATA CCGGCCAAAA CACGAAAAAC 
AGTCTTGGCT CCAGCTTTAA TTCCAGTAAT TATACTTCTT ATAGATTCCA GACATGTCTC 
ACCGACCAGA TTATTTCTGA GGCGCAGACG TGGTCACTTT CATCCCTCTT CAACTTCTCG 
TGGGTTGTGT CCTACTTTGT TATGGGTGCC TCTCGTATGA TCTTCAGGTA CGGTTGGTAT 
CTGGCAACTT TATCGCTTTT AAGGATTCCG AAGTGGATCT TCTTCAAGTT ACACCATGTT 
CAGTTCACTC TTTCCTTTTG GCTGATCCTG TTTGCCCTAG CCGTCATAGT CTTTGTGACT 
TACACCATCA TGAAGGAGAG AATTCTATCC CAGTACAAGA GGCTGACCCC GGAGTTCTTG 
CCGCTGGAAA ATACAGGTAA ATCTGGCTCC AGCGCCAATA TCAATGCAGC TTCCACTCAG 
TCAGCCAACG CGCCACCCGC CATAGGTTCC TCTACCACGG GTGCAAGCTC GATTATAGAC 
TCAAAAAAGC ATTCATTGAA AGATGGCAAC GAAAACGAAA CCTTTCTTTC ATCTTACCTT 
GACCAATTTC TCAGCGCCAT CAAAATCTTT GGCTATCTGG AAAAACCAGT CTTCCATGAC 
CTGACCAAGA ATATGAAAAC TCAAAAAATG GATGAGGGTG AGATTTTACT GTTGGACAGT 
ACTATTGGGT TTGCCATCGT CGTTGAAGGC ACCCTGCAGT TATATCATGA AGTAGATCAT 
TCAGATAAGG ACCATGGCGA TGAAACTGAC CACAGTGACA CGGATGGTCT TGACGACCAG 
GATAGAGATG AAGAAGACGA AGAAGAAGAT GATGACATTG ATAACTACGA CACCAAATCT 
TGTTCTTCTA ACCTCATCGA TGAAGAAGAT GAATCCGTTG GTTACATTCA TCTGAAAAAT 
GGACTGGGAA ATTTTCAACT GCTAAATACA GTCAAGCCAG GAAACCCGCT GACCTCCCTC 
GTGAGCATTT TAAACTTATT CACGCATTCC ATGTCCTCGT ATGGCAACAG TAACTTTCCC 
TCTGAGTTAT CGTCACCTAT AGACACAACG GTGTCGGTCA ACAATATGTT CTGCTCATCA 
GAGCAGAACT TCTCCAACAC AGATTCCATG ACAAACTCCA CCAACAGCTT TCCAACGTTC 
CCCTCTTCAA TGCCTAAACT TGTCGCCCGT GCGGCTACAG ACTGCACAAT TGGTATTATC 
CCACCTCAAT CGTTTGCCAA ATTAACCGCA AAATACCCAC GGTCTGCTTC CCATATCATA 
CAGATGGTTC TCACCAAGCT ATACCATGTT ACGTTCCAAA CTGCTCACGA CTACTTAGGT 
TTAACAAAGG AGATCATGGA TATCGAAGTT TTACTAAATA AATCCATCGT TTATGAATTG 
CCTTATTATT TGAAAGAGGC CGTTATTCGT AAGTTTAAAA CTGTAGATAA GTCGAGCGGA 
TCGGCTGATT TAGAACCGAA ACCCAAAAAT AGTAATGCTT CCTCTAAATT GAAGAAACCG 
CCCAAAGCGA AGCCATCTGA TGGTATCATT CAATCGCTTA AGATTGCTAA CGCCAACGCT 
AACACAAGTT CGAATTCCCT TTCACTGAAA CCAGAATTCA CCCACCATCC AAGTTCGAGA 
CATGTCGTAC TGGGTTCAAG AGACCAATTT AATCCTGGTG ATTTACTGTC AAATGTCCCA 
CTGTCTAGAA CTATGGATAT TCTGTCACCC AACCCAATCC ATAATAACAA CCGCAATAAA 
TCAAACGGTA TTAACACTTC TACCTCTAAC CAACATAAGC GATCTTCTAG GAGTAGCAGT 
AACAATGCTT CCGTACACTC TAAGAAGTTT TCTTCTTTGT CCCCAGAATT GCGCAACGCA 
CAACTTAGTA CATCGCCCTT GTCCTTGGAT AATACTTCTG TTCATGACCA TATCCACCCA 
AGTCCCGTTC ATTTGAAGGG ACGGGTCTCT CCAAGACCTA ATTTACTGCC CACAACATCC 
TTTTCTGCTG CCCAGGAAGA AACCGAGGAC TCTGCTTTAA GAATGGCTTT AGTTGAGGCA 
ATGTTAACAT ACTTGGGTGT AAACAAAAGC AATATGTCCG TTTCATCTTC TTCGATAGCA 
AATATGTCTT CTTTAAACTC TCCTCAACTT AATGAAATGT ATAGCCGCCG TCCAAGTAAC 
GCAAGTTTTC TGATGTCCCC TCACTGTACA CCATCTGACA TTTCCGTAGC TTCATCATTT 
GCCTCTCCTC AAACTCAACC GACAATGTTA AGAATCCTAC CCAAAGAATA CACTATCAGC 
AATAAAAGGC ATAATAAAAG TAAAAGCCAG GACAAAAAAA AACCTAGAGC TTACAAAGAG 
GAGTTGACAC CCAATTTGGA TTTCGAAGAT GTCAAGAAAG ACTTTGCACA AGGCATTCAG 
TTGAAGTTCT TTAAAAAGGG CACCACAATT GTTGAGCAGA ACGCTCGTGG TAAAGGCCTA 
TTCTATATCA TTTCCGGGAA AGTTAATGTG ACAACAAATT CGTCCTCGTC AGTAGTATCA 
TCAATGTCTA AACCAGAGCA GGTTTCAGCC CAAAGTTCAC ACAAGGGTGA AAATCCTCAT 
CATACTCAAC ATTTGTTATA CTCTGTAGGA TCTGGTGGTA TTGTGGGCTA CTTGTCCTCT 
CTAATTGGTT ACAAATCATT TGTTAATATC GTTGCCAAGA GCGATGTCTA TGTTGGATTT 
TTATCTAGTG CAACCTTAGA AAGATTGTTT GATAAATATT TCCTAATCTA CTTACGGATT 
TCTGATTCAT TAACCAAATT GCTATCTTCA AGGTTATTAA AGCTCGACCA TGCTTTAGAA 
TGGGTTCACT TGAGGGCTTC TGAAACTCTA TTTTCACAAG GTGACTCGGC TAACGGTATT 
TATGTTGTCC TGAATGGTAG ACTAAGGCAG CTACAACAGC AGTCATTGTC AAACTCCAAT 
ACAAGTTCCG AAGAAGTTGA AACTCAGAAC ATTATTCTAG GAGAACTGGC CCAGGGGGAG 
AGTTTTGGTG AAGTTGAAGT GCTTACCGCT ATGAATAGAT ATTCAACGAT AGTGGCAGTG 
AGGGATTCTG AATTAGCGAG AATTCCGAGA ACATTATTCG AACTATTAGC TCTTGAACAT 
CCATCTATCA TGATTAGAGT TTCCAGATTA GTTGCTAAAA AAATTGTCGG AGATAGAACT 
GTTCCAGCAT TAACAGGCGA TCCATTATCT ATAAAGGAAA ACGACTTCAC TTCTTTAATA 
CCTCCAACGA AAGCGTCTTA CTCTAGCTCT TTAAGTCATA AACCCCAAAA TATTACTTCT 
GGCACAATTA CTTTTAGAAC CATCACTATC TTACCAATTA CCAGTGGACT ACCTGTAGAA 
GCATTTGCTA TGAAGTTAGT GCAAGCTTTC AAACAGGTAG GAAGGACCAC GATTGGTTTA 
AATCAAAGAA CTACCCTAAC ACATTTAGGT CGTCATGCAT TTGACAGATT GTCCAAATTA 
AAACAAAGTG GTTACTTTGC TGAACTAGAA GAAATGTACC AGACTGTTGT TTATATATCA 
GATACACCAG TTAAATCTAA TTGGACAAGA ACCTGTATTG CGCAAGGTGA TTGTATTCTC 
TTACTAGCAG ATGCTAGATC TCCATCAGCC GAAATAGGAG AATATGAAAA GTTATTACTA 
AATTCAAAGA CTACCGCAAG GACCGAGTTA ATTCTTCTAC ACCCTGAAAG GTATGTGGAA 
CCAGGACTAA CCCATAAGTG GTTACGCTAT AGGCCATGGG TTCATTCGCA TCATCACATT 
CAATTTAGTT TGACAGGAAC AACCTTGATG AACGAAGGCA AAATGCACGT TTTGAATAAT 
GGTGCCTTGG CATTAATGGA TAAGTTGATT CAAACAGAAT TCAGTAGAAA AACTCAACAG 
AATATATCAA AACTACTTCC AGATTCTATC AAGAACACCG TAGAAAACTT TTCTTCAAGA 
TTCATGAAAA GTAAAAGACA ATATTACACA CCAGTTCATA GGCATAAAAA TGATTTTCTA 
AGATTGGCTA GAATTCTTTC TGGACAAGCT ATTGGACTTG TCCTCGGAGG AGGAGGTGCG 
AGAGGTATTA GTCATTTGGG TGTCATTCAA GCTATTGAAG AACAAGGTAT TCCGGTCGAC 
GTTATTGGAG GAACATCGAT TGGTTCCTTT GTTGGCGGTT TGTACGCGAA GGATTATGAT 
TTGGTGCCAA TTTACGGCCG CGTGAAGAAG TTTGCTGGTC GAATTTCCTC TATATGGAGA 
ATGTTAACAG ATTTAACCTG GCCGGTTACT TCTTATACTA CTGGTCACGA ATTTAATAGA 
GGTATTTGGA AAACTTTCGG GGATACAAGA ATTGAAGACT TCTGGATCCA GTATTATTGT 
AATTCTACTA ATATAACCGA TTCGGTTCAA GAAATACATT CCTTTGGTTA TGCGTGGAGG 
TACATCAGAG CTTCGATGTC TCTTGCTGGT CTTTTACCTC CTTTGGAAGA GAACGGGTCT 
ATGTTACTGG ATGGTGGTTA TGTTGATAAT TTACCAGTAA CTGAGATGAG AGCGCGTGGT 
TGCCAAACTA TATTTGCAGT AGATGTAGGT TCTGCAGATG ATAGAACGCC TATGGAATAT 
GGTGACTCCC TAAATGGGTT TTGGATCATT TTCAATAGAT GGAATCCATT TTCTTCTCAT 
CCAAACATAC CCAATATGGC AGAGATTCAA GTTAGATTGG GTTACGTCGC ATCAGTAAAT 
GCTTTAGAGA AAGCGAAAAA TACACCAGGT GTCGTTTATG TGAGACCACC TATTGAAGAA 
TATGCCACAT TAGACTTTAG CAAGTTCGAA GAAATTTATC ATGTGGGTGT AGACTATGGT 
CGTATCTTTT TGCAAGGGCT TATTGACGAC GACAAGATGC CATATATACC TGGTTCACAA 
GAAACCACTT TGAATTCTCA AGTTCCTGAG TTTTTATTAC ATAGAAGAAA TAGTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013652.1
CDS	1..5040
Translation	MRSMNCTTNNTNNTGQNTKNSLGSSFNSSNYTSYRFQTCLTDQIISEAQTWSLSSLFNFSWVVSYFVMGASRMIFRYGWYLATLSLLRIPKWIFFKLHHVQFTLSFWLILFALAVIVFVTYTIMKERILSQYKRLTPEFLPLENTGKSGSSANINAASTQSANAPPAIGSSTTGASSIIDSKKHSLKDGNENETFLSSYLDQFLSAIKIFGYLEKPVFHDLTKNMKTQKMDEGEILLLDSTIGFAIVVEGTLQLYHEVDHSDKDHGDETDHSDTDGLDDQDRDEEDEEEDDDIDNYDTKSCSSNLIDEEDESVGYIHLKNGLGNFQLLNTVKPGNPLTSLVSILNLFTHSMSSYGNSNFPSELSSPIDTTVSVNNMFCSSEQNFSNTDSMTNSTNSFPTFPSSMPKLVARAATDCTIGIIPPQSFAKLTAKYPRSASHIIQMVLTKLYHVTFQTAHDYLGLTKEIMDIEVLLNKSIVYELPYYLKEAVIRKFKTVDKSSGSADLEPKPKNSNASSKLKKPPKAKPSDGIIQSLKIANANANTSSNSLSLKPEFTHHPSSRHVVLGSRDQFNPGDLLSNVPLSRTMDILSPNPIHNNNRNKSNGINTSTSNQHKRSSRSSSNNASVHSKKFSSLSPELRNAQLSTSPLSLDNTSVHDHIHPSPVHLKGRVSPRPNLLPTTSFSAAQEETEDSALRMALVEAMLTYLGVNKSNMSVSSSSIANMSSLNSPQLNEMYSRRPSNASFLMSPHCTPSDISVASSFASPQTQPTMLRILPKEYTISNKRHNKSKSQDKKKPRAYKEELTPNLDFEDVKKDFAQGIQLKFFKKGTTIVEQNARGKGLFYIISGKVNVTTNSSSSVVSSMSKPEQVSAQSSHKGENPHHTQHLLYSVGSGGIVGYLSSLIGYKSFVNIVAKSDVYVGFLSSATLERLFDKYFLIYLRISDSLTKLLSSRLLKLDHALEWVHLRASETLFSQGDSANGIYVVLNGRLRQLQQQSLSNSNTSSEEVETQNIILGELAQGESFGEVEVLTAMNRYSTIVAVRDSELARIPRTLFELLALEHPSIMIRVSRLVAKKIVGDRTVPALTGDPLSIKENDFTSLIPPTKASYSSSLSHKPQNITSGTITFRTITILPITSGLPVEAFAMKLVQAFKQVGRTTIGLNQRTTLTHLGRHAFDRLSKLKQSGYFAELEEMYQTVVYISDTPVKSNWTRTCIAQGDCILLLADARSPSAEIGEYEKLLLNSKTTARTELILLHPERYVEPGLTHKWLRYRPWVHSHHHIQFSLTGTTLMNEGKMHVLNNGALALMDKLIQTEFSRKTQQNISKLLPDSIKNTVENFSSRFMKSKRQYYTPVHRHKNDFLRLARILSGQAIGLVLGGGGARGISHLGVIQAIEEQGIPVDVIGGTSIGSFVGGLYAKDYDLVPIYGRVKKFAGRISSIWRMLTDLTWPVTSYTTGHEFNRGIWKTFGDTRIEDFWIQYYCNSTNITDSVQEIHSFGYAWRYIRASMSLAGLLPPLEENGSMLLDGGYVDNLPVTEMRARGCQTIFAVDVGSADDRTPMEYGDSLNGFWIIFNRWNPFSSHPNIPNMAEIQVRLGYVASVNALEKAKNTPGVVYVRPPIEEYATLDFSKFEEIYHVGVDYGRIFLQGLIDDDKMPYIPGSQETTLNSQVPEFLLHRRNSI

Target ORF information:

RefSeq Version	NM_001182418.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C lysophospholipase (NTE1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182418.1

ORF Insert Sequence:

ATGCGTTCAA TGAATTGCAC TACGAACAAC ACCAACAATA CCGGCCAAAA CACGAAAAAC 
AGTCTTGGCT CCAGCTTTAA TTCCAGTAAT TATACTTCTT ATAGATTCCA GACATGTCTC 
ACCGACCAGA TTATTTCTGA GGCGCAGACG TGGTCACTTT CATCCCTCTT CAACTTCTCG 
TGGGTTGTGT CCTACTTTGT TATGGGTGCC TCTCGTATGA TCTTCAGGTA CGGTTGGTAT 
CTGGCAACTT TATCGCTTTT AAGGATTCCG AAGTGGATCT TCTTCAAGTT ACACCATGTT 
CAGTTCACTC TTTCCTTTTG GCTGATCCTG TTTGCCCTAG CCGTCATAGT CTTTGTGACT 
TACACCATCA TGAAGGAGAG AATTCTATCC CAGTACAAGA GGCTGACCCC GGAGTTCTTG 
CCGCTGGAAA ATACAGGTAA ATCTGGCTCC AGCGCCAATA TCAATGCAGC TTCCACTCAG 
TCAGCCAACG CGCCACCCGC CATAGGTTCC TCTACCACGG GTGCAAGCTC GATTATAGAC 
TCAAAAAAGC ATTCATTGAA AGATGGCAAC GAAAACGAAA CCTTTCTTTC ATCTTACCTT 
GACCAATTTC TCAGCGCCAT CAAAATCTTT GGCTATCTGG AAAAACCAGT CTTCCATGAC 
CTGACCAAGA ATATGAAAAC TCAAAAAATG GATGAGGGTG AGATTTTACT GTTGGACAGT 
ACTATTGGGT TTGCCATCGT CGTTGAAGGC ACCCTGCAGT TATATCATGA AGTAGATCAT 
TCAGATAAGG ACCATGGCGA TGAAACTGAC CACAGTGACA CGGATGGTCT TGACGACCAG 
GATAGAGATG AAGAAGACGA AGAAGAAGAT GATGACATTG ATAACTACGA CACCAAATCT 
TGTTCTTCTA ACCTCATCGA TGAAGAAGAT GAATCCGTTG GTTACATTCA TCTGAAAAAT 
GGACTGGGAA ATTTTCAACT GCTAAATACA GTCAAGCCAG GAAACCCGCT GACCTCCCTC 
GTGAGCATTT TAAACTTATT CACGCATTCC ATGTCCTCGT ATGGCAACAG TAACTTTCCC 
TCTGAGTTAT CGTCACCTAT AGACACAACG GTGTCGGTCA ACAATATGTT CTGCTCATCA 
GAGCAGAACT TCTCCAACAC AGATTCCATG ACAAACTCCA CCAACAGCTT TCCAACGTTC 
CCCTCTTCAA TGCCTAAACT TGTCGCCCGT GCGGCTACAG ACTGCACAAT TGGTATTATC 
CCACCTCAAT CGTTTGCCAA ATTAACCGCA AAATACCCAC GGTCTGCTTC CCATATCATA 
CAGATGGTTC TCACCAAGCT ATACCATGTT ACGTTCCAAA CTGCTCACGA CTACTTAGGT 
TTAACAAAGG AGATCATGGA TATCGAAGTT TTACTAAATA AATCCATCGT TTATGAATTG 
CCTTATTATT TGAAAGAGGC CGTTATTCGT AAGTTTAAAA CTGTAGATAA GTCGAGCGGA 
TCGGCTGATT TAGAACCGAA ACCCAAAAAT AGTAATGCTT CCTCTAAATT GAAGAAACCG 
CCCAAAGCGA AGCCATCTGA TGGTATCATT CAATCGCTTA AGATTGCTAA CGCCAACGCT 
AACACAAGTT CGAATTCCCT TTCACTGAAA CCAGAATTCA CCCACCATCC AAGTTCGAGA 
CATGTCGTAC TGGGTTCAAG AGACCAATTT AATCCTGGTG ATTTACTGTC AAATGTCCCA 
CTGTCTAGAA CTATGGATAT TCTGTCACCC AACCCAATCC ATAATAACAA CCGCAATAAA 
TCAAACGGTA TTAACACTTC TACCTCTAAC CAACATAAGC GATCTTCTAG GAGTAGCAGT 
AACAATGCTT CCGTACACTC TAAGAAGTTT TCTTCTTTGT CCCCAGAATT GCGCAACGCA 
CAACTTAGTA CATCGCCCTT GTCCTTGGAT AATACTTCTG TTCATGACCA TATCCACCCA 
AGTCCCGTTC ATTTGAAGGG ACGGGTCTCT CCAAGACCTA ATTTACTGCC CACAACATCC 
TTTTCTGCTG CCCAGGAAGA AACCGAGGAC TCTGCTTTAA GAATGGCTTT AGTTGAGGCA 
ATGTTAACAT ACTTGGGTGT AAACAAAAGC AATATGTCCG TTTCATCTTC TTCGATAGCA 
AATATGTCTT CTTTAAACTC TCCTCAACTT AATGAAATGT ATAGCCGCCG TCCAAGTAAC 
GCAAGTTTTC TGATGTCCCC TCACTGTACA CCATCTGACA TTTCCGTAGC TTCATCATTT 
GCCTCTCCTC AAACTCAACC GACAATGTTA AGAATCCTAC CCAAAGAATA CACTATCAGC 
AATAAAAGGC ATAATAAAAG TAAAAGCCAG GACAAAAAAA AACCTAGAGC TTACAAAGAG 
GAGTTGACAC CCAATTTGGA TTTCGAAGAT GTCAAGAAAG ACTTTGCACA AGGCATTCAG 
TTGAAGTTCT TTAAAAAGGG CACCACAATT GTTGAGCAGA ACGCTCGTGG TAAAGGCCTA 
TTCTATATCA TTTCCGGGAA AGTTAATGTG ACAACAAATT CGTCCTCGTC AGTAGTATCA 
TCAATGTCTA AACCAGAGCA GGTTTCAGCC CAAAGTTCAC ACAAGGGTGA AAATCCTCAT 
CATACTCAAC ATTTGTTATA CTCTGTAGGA TCTGGTGGTA TTGTGGGCTA CTTGTCCTCT 
CTAATTGGTT ACAAATCATT TGTTAATATC GTTGCCAAGA GCGATGTCTA TGTTGGATTT 
TTATCTAGTG CAACCTTAGA AAGATTGTTT GATAAATATT TCCTAATCTA CTTACGGATT 
TCTGATTCAT TAACCAAATT GCTATCTTCA AGGTTATTAA AGCTCGACCA TGCTTTAGAA 
TGGGTTCACT TGAGGGCTTC TGAAACTCTA TTTTCACAAG GTGACTCGGC TAACGGTATT 
TATGTTGTCC TGAATGGTAG ACTAAGGCAG CTACAACAGC AGTCATTGTC AAACTCCAAT 
ACAAGTTCCG AAGAAGTTGA AACTCAGAAC ATTATTCTAG GAGAACTGGC CCAGGGGGAG 
AGTTTTGGTG AAGTTGAAGT GCTTACCGCT ATGAATAGAT ATTCAACGAT AGTGGCAGTG 
AGGGATTCTG AATTAGCGAG AATTCCGAGA ACATTATTCG AACTATTAGC TCTTGAACAT 
CCATCTATCA TGATTAGAGT TTCCAGATTA GTTGCTAAAA AAATTGTCGG AGATAGAACT 
GTTCCAGCAT TAACAGGCGA TCCATTATCT ATAAAGGAAA ACGACTTCAC TTCTTTAATA 
CCTCCAACGA AAGCGTCTTA CTCTAGCTCT TTAAGTCATA AACCCCAAAA TATTACTTCT 
GGCACAATTA CTTTTAGAAC CATCACTATC TTACCAATTA CCAGTGGACT ACCTGTAGAA 
GCATTTGCTA TGAAGTTAGT GCAAGCTTTC AAACAGGTAG GAAGGACCAC GATTGGTTTA 
AATCAAAGAA CTACCCTAAC ACATTTAGGT CGTCATGCAT TTGACAGATT GTCCAAATTA 
AAACAAAGTG GTTACTTTGC TGAACTAGAA GAAATGTACC AGACTGTTGT TTATATATCA 
GATACACCAG TTAAATCTAA TTGGACAAGA ACCTGTATTG CGCAAGGTGA TTGTATTCTC 
TTACTAGCAG ATGCTAGATC TCCATCAGCC GAAATAGGAG AATATGAAAA GTTATTACTA 
AATTCAAAGA CTACCGCAAG GACCGAGTTA ATTCTTCTAC ACCCTGAAAG GTATGTGGAA 
CCAGGACTAA CCCATAAGTG GTTACGCTAT AGGCCATGGG TTCATTCGCA TCATCACATT 
CAATTTAGTT TGACAGGAAC AACCTTGATG AACGAAGGCA AAATGCACGT TTTGAATAAT 
GGTGCCTTGG CATTAATGGA TAAGTTGATT CAAACAGAAT TCAGTAGAAA AACTCAACAG 
AATATATCAA AACTACTTCC AGATTCTATC AAGAACACCG TAGAAAACTT TTCTTCAAGA 
TTCATGAAAA GTAAAAGACA ATATTACACA CCAGTTCATA GGCATAAAAA TGATTTTCTA 
AGATTGGCTA GAATTCTTTC TGGACAAGCT ATTGGACTTG TCCTCGGAGG AGGAGGTGCG 
AGAGGTATTA GTCATTTGGG TGTCATTCAA GCTATTGAAG AACAAGGTAT TCCGGTCGAC 
GTTATTGGAG GAACATCGAT TGGTTCCTTT GTTGGCGGTT TGTACGCGAA GGATTATGAT 
TTGGTGCCAA TTTACGGCCG CGTGAAGAAG TTTGCTGGTC GAATTTCCTC TATATGGAGA 
ATGTTAACAG ATTTAACCTG GCCGGTTACT TCTTATACTA CTGGTCACGA ATTTAATAGA 
GGTATTTGGA AAACTTTCGG GGATACAAGA ATTGAAGACT TCTGGATCCA GTATTATTGT 
AATTCTACTA ATATAACCGA TTCGGTTCAA GAAATACATT CCTTTGGTTA TGCGTGGAGG 
TACATCAGAG CTTCGATGTC TCTTGCTGGT CTTTTACCTC CTTTGGAAGA GAACGGGTCT 
ATGTTACTGG ATGGTGGTTA TGTTGATAAT TTACCAGTAA CTGAGATGAG AGCGCGTGGT 
TGCCAAACTA TATTTGCAGT AGATGTAGGT TCTGCAGATG ATAGAACGCC TATGGAATAT 
GGTGACTCCC TAAATGGGTT TTGGATCATT TTCAATAGAT GGAATCCATT TTCTTCTCAT 
CCAAACATAC CCAATATGGC AGAGATTCAA GTTAGATTGG GTTACGTCGC ATCAGTAAAT 
GCTTTAGAGA AAGCGAAAAA TACACCAGGT GTCGTTTATG TGAGACCACC TATTGAAGAA 
TATGCCACAT TAGACTTTAG CAAGTTCGAA GAAATTTATC ATGTGGGTGT AGACTATGGT 
CGTATCTTTT TGCAAGGGCT TATTGACGAC GACAAGATGC CATATATACC TGGTTCACAA 
GAAACCACTT TGAATTCTCA AGTTCCTGAG TTTTTATTAC ATAGAAGAAA TAGTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NTE1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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