ORC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ORC1
Entrez Gene ID	854937
Full Name	origin recognition complex subunit 1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182424.1	NP_013646.1	origin recognition complex subunit 1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ORC1	NP_013646.1
Kluyveromyces lactis	KLLA0B05016g	XP_451757.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the diminished cell-cycle changes in nucleosome occupancy around origins in the orc1-161 mutant are associated with an abnormal global origin usage profile, suggesting that proper establishment of nucleosome occupancy around origins is a critical step for regulation of global origin activities. Our work thus establishes nucleosome occupancy as a novel and key chromatin parameter for proper origin regulation.
Title: Nucleosome occupancy as a novel chromatin parameter for replication origin functions.

Specific ORC1 to DNA replication origins depends on highly conserved basic residues.
Title: Specific binding of eukaryotic ORC to DNA replication origins depends on highly conserved basic residues.

Data indicate that Cdt1-MCM2-7 complex activates ORC/Cdc6 ATP-hydrolysis to promote helicase loading.
Title: An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly.

The functional specialization of Sir3,as a silencing protein was facilitated by the tandem duplication of the OIR domain in the Sir1 family, allowing distinct Sir1-Sir3 and Sir1-Orc1 interactions through OIR-BAH domain interactions.
Title: Elaboration, diversification and regulation of the Sir1 family of silencing proteins in Saccharomyces.

Coimmunoprecipitation assay confirmed that Cdt1p interacted with Orc6p, as well as with Orc1p and Orc2p.
Title: Interaction between ORC and Cdt1p of Saccharomyces cerevisiae.

The following ORC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ORC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03936	NM_001182424.1 Latest version!	Saccharomyces cerevisiae S288C origin recognition complex subunit 1 (ORC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ORC1 ( NM_001182424.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013646.1 Saccharomyces cerevisiae S288C origin recognition complex subunit 1 (ORC1), partial mRNA.

Price & Availability↑

CloneID	OSi03936
Clone ID Related Accession (Same CDS sequence)	NM_001182424.1
Accession Version	NM_001182424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	origin recognition complex subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAAAAA CGTTGAAGGA TTTACAGGGT TGGGAGATAA TAACAACTGA TGAGCAGGGA 
AATATAATCG ATGGAGGTCA GAAGAGATTA CGCCGAAGAG GTGCAAAAAC TGAACATTAC 
TTAAAGAGAA GTTCTGATGG AATTAAACTA GGTCGTGGTG ATAGTGTAGT CATGCACAAC 
GAAGCCGCTG GGACTTACTC CGTTTATATG ATCCAGGAGT TGAGACTTAA TACATTAAAT 
AATGTTGTCG AACTCTGGGC TCTCACCTAT TTACGATGGT TTGAAGTCAA TCCTTTAGCT 
CATTATAGGC AGTTTAATCC TGACGCTAAC ATTTTGAATC GTCCTTTAAA TTATTACAAT 
AAACTGTTTT CTGAAACTGC AAATAAAAAT GAACTGTATC TCACTGCAGA ATTAGCCGAA 
TTGCAGCTAT TTAACTTTAT CAGGGTTGCC AACGTAATGG ATGGAAGCAA ATGGGAAGTA 
TTGAAAGGAA ATGTCGATCC AGAAAGAGAC TTTACAGTTC GTTATATTTG TGAGCCGACT 
GGGGAGAAAT TTGTGGACAT TAATATTGAG GATGTCAAAG CTTACATAAA GAAAGTGGAG 
CCAAGGGAAG CCCAGGAATA TTTGAAAGAT TTAACACTTC CATCAAAGAA GAAAGAGATC 
AAAAGAGGTC CTCAAAAGAA AGATAAGGCT ACTCAAACGG CACAAATTTC AGACGCAGAA 
ACAAGAGCTA CAGATATAAC GGATAATGAG GACGGTAATG AAGATGAATC ATCTGATTAT 
GAAAGTCCGT CAGATATCGA CGTTAGCGAG GATATGGACA GCGGTGAAAT ATCCGCAGAT 
GAGCTTGAGG AAGAAGAAGA CGAAGAAGAA GACGAAGACG AAGAAGAGAA AGAAGCTAGG 
CATACAAATT CACCAAGGAA AAGAGGCCGT AAGATAAAAC TAGGTAAAGA TGATATTGAC 
GCTTCTGTAC AACCTCCCCC CAAAAAAAGA GGTCGTAAAC CTAAAGATCC TAGTAAACCG 
CGTCAGATGC TATTGATATC TTCATGCCGT GCAAATAATA CTCCTGTGAT TAGGAAATTT 
ACAAAAAAGA ATGTTGCTAG GGCGAAAAAG AAATATACCC CGTTTTCGAA AAGATTTAAA 
TCTATAGCTG CAATACCAGA TTTAACTTCA TTACCTGAAT TTTACGGAAA TTCTTCGGAA 
TTGATGGCAT CAAGGTTTGA AAACAAATTA AAAACAACCC AAAAGCATCA GATTGTAGAA 
ACAATTTTTT CTAAAGTCAA AAAACAGTTG AACTCTTCGT ATGTCAAAGA AGAAATATTG 
AAGTCTGCAA ATTTCCAAGA TTATTTACCG GCTAGGGAGA ATGAATTCGC CTCAATTTAT 
TTAAGTGCAT ATAGTGCCAT TGAGTCCGAC TCCGCTACTA CTATATACGT GGCTGGTACG 
CCTGGTGTAG GGAAAACTTT AACCGTAAGG GAAGTCGTAA AGGAACTACT ATCGTCTTCT 
GCACAACGAG AAATACCAGA CTTTCTTTAT GTGGAAATAA ATGGATTGAA AATGGTAAAA 
CCCACAGACT GTTACGAAAC TTTATGGAAC AAAGTGTCAG GAGAAAGGTT AACATGGGCA 
GCTTCAATGG AGTCACTAGA GTTTTACTTT AAAAGAGTTC CAAAAAATAA GAAGAAAACC 
ATTGTAGTCT TGTTGGACGA ACTCGATGCC ATGGTAACGA AATCTCAAGA TATTATGTAC 
AATTTTTTCA ATTGGACTAC TTACGAAAAT GCCAAACTTA TTGTCATTGC AGTAGCCAAT 
ACAATGGACT TACCAGAACG TCAGCTAGGC AATAAGATTA CTTCAAGAAT TGGGTTTACC 
AGAATTATGT TCACTGGGTA TACGCACGAA GAGCTAAAAA ATATCATTGA TTTAAGACTG 
AAGGGGTTGA ACGACTCATT TTTCTATGTT GATACAAAAA CTGGCAATGC TATTTTGATT 
GATGCGGCTG GAAACGACAC TACAGTTAAG CAAACGTTGC CTGAAGACGT GAGGAAAGTT 
CGCTTAAGAA TGAGTGCTGA TGCCATTGAA ATAGCTTCGA GAAAAGTAGC AAGTGTTAGT 
GGTGATGCAA GAAGAGCATT GAAGGTTTGT AAAAGAGCAG CTGAAATTGC TGAAAAACAC 
TATATGGCTA AGCATGGTTA TGGATATGAT GGAAAGACGG TTATTGAAGA TGAAAATGAG 
GAGCAAATAT ACGATGATGA AGACAAGGAT CTTATTGAAA GTAACAAAGC CAAAGACGAT 
AATGATGACG ATGATGACAA TGATGGGGTA CAAACAGTTC ACATCACGCA CGTTATGAAA 
GCCTTAAACG AAACTTTAAA TTCTCATGTA ATTACGTTTA TGACGCGACT TTCATTTACA 
GCAAAACTGT TTATTTATGC ATTATTAAAC TTGATGAAAA AGAACGGATC TCAAGAGCAA 
GAACTGGGCG ATATTGTCGA TGAAATCAAG TTACTTATTG AAGTAAATGG CAGTAATAAG 
TTTGTCATGG AGATAGCCAA AACATTGTTC CAACAGGGAA GTGATAATAT TTCTGAACAA 
TTGAGAATTA TATCATGGGA TTTCGTTCTC AATCAGTTAC TTGACGCGGG AATATTGTTT 
AAACAAACTA TGAAGAACGA TAGAATATGT TGTGTCAAGC TAAATATATC AGTAGAAGAA 
GCCAAAAGAG CCATGAATGA GGATGAGACA TTGAGAAATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013646.1
CDS	1..2745
Translation	MAKTLKDLQGWEIITTDEQGNIIDGGQKRLRRRGAKTEHYLKRSSDGIKLGRGDSVVMHNEAAGTYSVYMIQELRLNTLNNVVELWALTYLRWFEVNPLAHYRQFNPDANILNRPLNYYNKLFSETANKNELYLTAELAELQLFNFIRVANVMDGSKWEVLKGNVDPERDFTVRYICEPTGEKFVDINIEDVKAYIKKVEPREAQEYLKDLTLPSKKKEIKRGPQKKDKATQTAQISDAETRATDITDNEDGNEDESSDYESPSDIDVSEDMDSGEISADELEEEEDEEEDEDEEEKEARHTNSPRKRGRKIKLGKDDIDASVQPPPKKRGRKPKDPSKPRQMLLISSCRANNTPVIRKFTKKNVARAKKKYTPFSKRFKSIAAIPDLTSLPEFYGNSSELMASRFENKLKTTQKHQIVETIFSKVKKQLNSSYVKEEILKSANFQDYLPARENEFASIYLSAYSAIESDSATTIYVAGTPGVGKTLTVREVVKELLSSSAQREIPDFLYVEINGLKMVKPTDCYETLWNKVSGERLTWAASMESLEFYFKRVPKNKKKTIVVLLDELDAMVTKSQDIMYNFFNWTTYENAKLIVIAVANTMDLPERQLGNKITSRIGFTRIMFTGYTHEELKNIIDLRLKGLNDSFFYVDTKTGNAILIDAAGNDTTVKQTLPEDVRKVRLRMSADAIEIASRKVASVSGDARRALKVCKRAAEIAEKHYMAKHGYGYDGKTVIEDENEEQIYDDEDKDLIESNKAKDDNDDDDDNDGVQTVHITHVMKALNETLNSHVITFMTRLSFTAKLFIYALLNLMKKNGSQEQELGDIVDEIKLLIEVNGSNKFVMEIAKTLFQQGSDNISEQLRIISWDFVLNQLLDAGILFKQTMKNDRICCVKLNISVEEAKRAMNEDETLRNL

Target ORF information:

RefSeq Version	NM_001182424.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C origin recognition complex subunit 1 (ORC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182424.1

ORF Insert Sequence:

ATGGCAAAAA CGTTGAAGGA TTTACAGGGT TGGGAGATAA TAACAACTGA TGAGCAGGGA 
AATATAATCG ATGGAGGTCA GAAGAGATTA CGCCGAAGAG GTGCAAAAAC TGAACATTAC 
TTAAAGAGAA GTTCTGATGG AATTAAACTA GGTCGTGGTG ATAGTGTAGT CATGCACAAC 
GAAGCCGCTG GGACTTACTC CGTTTATATG ATCCAGGAGT TGAGACTTAA TACATTAAAT 
AATGTTGTCG AACTCTGGGC TCTCACCTAT TTACGATGGT TTGAAGTCAA TCCTTTAGCT 
CATTATAGGC AGTTTAATCC TGACGCTAAC ATTTTGAATC GTCCTTTAAA TTATTACAAT 
AAACTGTTTT CTGAAACTGC AAATAAAAAT GAACTGTATC TCACTGCAGA ATTAGCCGAA 
TTGCAGCTAT TTAACTTTAT CAGGGTTGCC AACGTAATGG ATGGAAGCAA ATGGGAAGTA 
TTGAAAGGAA ATGTCGATCC AGAAAGAGAC TTTACAGTTC GTTATATTTG TGAGCCGACT 
GGGGAGAAAT TTGTGGACAT TAATATTGAG GATGTCAAAG CTTACATAAA GAAAGTGGAG 
CCAAGGGAAG CCCAGGAATA TTTGAAAGAT TTAACACTTC CATCAAAGAA GAAAGAGATC 
AAAAGAGGTC CTCAAAAGAA AGATAAGGCT ACTCAAACGG CACAAATTTC AGACGCAGAA 
ACAAGAGCTA CAGATATAAC GGATAATGAG GACGGTAATG AAGATGAATC ATCTGATTAT 
GAAAGTCCGT CAGATATCGA CGTTAGCGAG GATATGGACA GCGGTGAAAT ATCCGCAGAT 
GAGCTTGAGG AAGAAGAAGA CGAAGAAGAA GACGAAGACG AAGAAGAGAA AGAAGCTAGG 
CATACAAATT CACCAAGGAA AAGAGGCCGT AAGATAAAAC TAGGTAAAGA TGATATTGAC 
GCTTCTGTAC AACCTCCCCC CAAAAAAAGA GGTCGTAAAC CTAAAGATCC TAGTAAACCG 
CGTCAGATGC TATTGATATC TTCATGCCGT GCAAATAATA CTCCTGTGAT TAGGAAATTT 
ACAAAAAAGA ATGTTGCTAG GGCGAAAAAG AAATATACCC CGTTTTCGAA AAGATTTAAA 
TCTATAGCTG CAATACCAGA TTTAACTTCA TTACCTGAAT TTTACGGAAA TTCTTCGGAA 
TTGATGGCAT CAAGGTTTGA AAACAAATTA AAAACAACCC AAAAGCATCA GATTGTAGAA 
ACAATTTTTT CTAAAGTCAA AAAACAGTTG AACTCTTCGT ATGTCAAAGA AGAAATATTG 
AAGTCTGCAA ATTTCCAAGA TTATTTACCG GCTAGGGAGA ATGAATTCGC CTCAATTTAT 
TTAAGTGCAT ATAGTGCCAT TGAGTCCGAC TCCGCTACTA CTATATACGT GGCTGGTACG 
CCTGGTGTAG GGAAAACTTT AACCGTAAGG GAAGTCGTAA AGGAACTACT ATCGTCTTCT 
GCACAACGAG AAATACCAGA CTTTCTTTAT GTGGAAATAA ATGGATTGAA AATGGTAAAA 
CCCACAGACT GTTACGAAAC TTTATGGAAC AAAGTGTCAG GAGAAAGGTT AACATGGGCA 
GCTTCAATGG AGTCACTAGA GTTTTACTTT AAAAGAGTTC CAAAAAATAA GAAGAAAACC 
ATTGTAGTCT TGTTGGACGA ACTCGATGCC ATGGTAACGA AATCTCAAGA TATTATGTAC 
AATTTTTTCA ATTGGACTAC TTACGAAAAT GCCAAACTTA TTGTCATTGC AGTAGCCAAT 
ACAATGGACT TACCAGAACG TCAGCTAGGC AATAAGATTA CTTCAAGAAT TGGGTTTACC 
AGAATTATGT TCACTGGGTA TACGCACGAA GAGCTAAAAA ATATCATTGA TTTAAGACTG 
AAGGGGTTGA ACGACTCATT TTTCTATGTT GATACAAAAA CTGGCAATGC TATTTTGATT 
GATGCGGCTG GAAACGACAC TACAGTTAAG CAAACGTTGC CTGAAGACGT GAGGAAAGTT 
CGCTTAAGAA TGAGTGCTGA TGCCATTGAA ATAGCTTCGA GAAAAGTAGC AAGTGTTAGT 
GGTGATGCAA GAAGAGCATT GAAGGTTTGT AAAAGAGCAG CTGAAATTGC TGAAAAACAC 
TATATGGCTA AGCATGGTTA TGGATATGAT GGAAAGACGG TTATTGAAGA TGAAAATGAG 
GAGCAAATAT ACGATGATGA AGACAAGGAT CTTATTGAAA GTAACAAAGC CAAAGACGAT 
AATGATGACG ATGATGACAA TGATGGGGTA CAAACAGTTC ACATCACGCA CGTTATGAAA 
GCCTTAAACG AAACTTTAAA TTCTCATGTA ATTACGTTTA TGACGCGACT TTCATTTACA 
GCAAAACTGT TTATTTATGC ATTATTAAAC TTGATGAAAA AGAACGGATC TCAAGAGCAA 
GAACTGGGCG ATATTGTCGA TGAAATCAAG TTACTTATTG AAGTAAATGG CAGTAATAAG 
TTTGTCATGG AGATAGCCAA AACATTGTTC CAACAGGGAA GTGATAATAT TTCTGAACAA 
TTGAGAATTA TATCATGGGA TTTCGTTCTC AATCAGTTAC TTGACGCGGG AATATTGTTT 
AAACAAACTA TGAAGAACGA TAGAATATGT TGTGTCAAGC TAAATATATC AGTAGAAGAA 
GCCAAAAGAG CCATGAATGA GGATGAGACA TTGAGAAATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ORC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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