PHO84 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Molecular dynamics simulations of Pho84 in which a residue adjacent to Tyr(179), Asp(178), is protonated revealed a conformational change in Pho84 from an open, inward-facing state to an occluded state.
Title: Key Residues and Phosphate Release Routes in the Saccharomyces cerevisiae Pho84 Transceptor: THE ROLE OF TYR179 IN FUNCTIONAL REGULATION.

The phosphate-binding site residues were shown to be well conserved between all the plant PHT1 proteins and Saccharomyces cerevisiae PHO84.
Title: The conservation of phosphate-binding residues among PHT1 transporters suggests that distinct transport affinities are unlikely to result from differences in the phosphate-binding site.

Gcn5 is required for activation of nuclear genes and lifespan extension in the retrograde response.These results, along with the finding that the histone deacetylase Sir2 was required for a robust retrograde response informed a bioinformatics screen that reduced to four the candidate genes causal for longevity .Of the four, only deletion of PHO84 suppressed lifespan extension
Title: Identification of the Target of the Retrograde Response that Mediates Replicative Lifespan Extension in Saccharomyces cerevisiae.

The role of Pho84 in phosphate signalling is discussed. [review]
Title: Conservation of PHO pathway in ascomycetes and the role of Pho84.

PHO84 silencing uses antisense transcription via the promoter rather than the static accumulation of antisense RNAs at the repressed gene. PHO84 antisense RNA acts as a bimodal switch.
Title: Bimodal expression of PHO84 is modulated by early termination of antisense transcription.