VID28 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VID28
Entrez Gene ID	854795
Full Name	glucose-induced degradation complex subunit VID28
Synonyms	GID5,YIL017W
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179367.3	NP_012247.3	glucose-induced degradation complex subunit VID28

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	VID28	NP_012247.3
Eremothecium gossypii	AGOS_AGR238C	NP_986904.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vid28 protein is required for the association of vacuole import and degradation (Vid) vesicles with actin patches and the retention of Vid vesicle proteins in the intracellular fraction.
Title: Vid28 protein is required for the association of vacuole import and degradation (Vid) vesicles with actin patches and the retention of Vid vesicle proteins in the intracellular fraction.

Three members of the VID and GID gene families, VID30/GID1, GID2, and VID28/GID5 are needed for the rapamycin or nitrogen starvation-induced degradation of the high-affinity hexose transporter Hxt7p is shown here.
Title: Components of the Vid30c are needed for the rapamycin-induced degradation of the high-affinity hexose transporter Hxt7p in Saccharomyces cerevisiae.

The following VID28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VID28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03815	NM_001179367.3 Latest version!	Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID28 (VID28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VID28 ( NM_001179367.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012247.3 Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID28 (VID28), partial mRNA.

Price & Availability↑

CloneID	OSi03815
Clone ID Related Accession (Same CDS sequence)	NM_001179367.3
Accession Version	NM_001179367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	glucose-induced degradation complex subunit VID28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). On Jul 30, 2012 this sequence version replaced NM_001179367.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACGGTGG CTTATTCCCT AGAGAATCTC AAAAAGATTA GTAATTCCCT GGTGGGAGAC 
CAGTTGGCTA AGGTAGATTA CTTTTTGGCT CCAAAGTGCC AGATTTTTCA ATGTTTGTTG 
TCCATTGAGC AAAGTGACGG TGTAGAGTTA AAAAATGCGA AATTAGATCT GCTATATACC 
CTTTTACATC TAGAACCGCA ACAAAGGGAT ATAGTAGGCA CATATTACTT TGATATTGTG 
TCCGCAATCT ATAAGTCCAT GAGTTTGGCA AGCAGTTTTA CTAAAAATAA TTCTTCGACA 
AACTATAAGT ACATAAAGCT TCTAAATTTG TGTGCAGGAG TTTATCCAAA TTGCGGATTC 
CCAGACTTGC AATACCTCCA GAACGGGTTC ATCCAGTTAG TCAACCATAA ATTTTTGAGG 
AGCAAATGTA AAATTGATGA GGTGGTCACG ATTATTGAAT TATTGAAGTT GTTTTTACTT 
GTGGACGAGA AAAATTGCAG CGACTTCAAC AAGAGCAAGT TTATGGAGGA AGAGAGAGAA 
GTGACAGAAA CTTCACACTA CCAAGATTTT AAAATGGCAG AATCCCTTGA ACACATAATA 
GTTAAAATAT CATCGAAGTA CTTGGACCAA ATAAGCCTCA AGTATATAGT GAGGCTAAAA 
GTATCCAGGC CAGCGTCACC ATCCAGCGTC AAGAACGATC CGTTTGACAA TAAAGGGGTC 
GACTGCACCC GTGCCATACC TAAAAAAATC AATATCTCAA ACATGTATGA TTCTAGTTTA 
CTCTCATTGG CACTCCTTCT ATATCTAAGA TATCATTATA TGATACCGGG TGATAGGAAA 
CTGCGGAATG ATGCGACGTT CAAGATGTTC GTTCTGGGTC TTTTGAAGAG TAATGACGTT 
AACATTAGGT GCGTGGCCCT GAAGTTTTTG TTACAACCGT ACTTTACAGA GGATAAGAAA 
TGGGAAGATA CGCGTACACT CGAGAAAATA CTACCCTATT TAGTTAAGTC ATTTAATTAT 
GACCCGTTGC CTTGGTGGTT TGATCCATTC GATATGCTGG ATTCATTGAT TGTGTTGTAT 
AATGAAATTA CTCCAATGAA TAATCCTGTG CTCACCACGC TAGCTCATAC AAACGTTATA 
TTTTGCATTC TATCCCGTTT TGCGCAGTGT CTTTCTCTAC CACAACACAA TGAAGCTACT 
TTGAAAACAA CTACAAAATT CATTAAAATA TGTGCGTCAT TTGCAGCATC GGACGAAAAA 
TACCGTCTTT TACTGTTGAA TGACACATTG TTGTTGAATC ATTTAGAATA CGGGTTGGAA 
TCCCACATTA CTTTGATACA GGATTTTATA TCTTTGAAAG ATGAGATAAA GGAAACAACT 
ACGGAAAGTC ATTCTATGTG TTTACCCCCA ATATATGATC ACGATTTCGT GGCAGCTTGG 
TTACTATTGT TGAAATCATT CTCGAGAAGT GTTTCAGCGC TTAGGACGAC GTTGAAGAGA 
AATAAAATTG CACAGCTGCT TTTACAGATA CTAAGTAAAA CATATACGTT GACCAAGGAA 
TGCTATTTTG CTGGACAAGA TTTTATGAAA CCGGAAATTA TGATTATGGG CATTACTCTG 
GGTAGTATTT GTAACTTCGT GGTGGAGTTC TCTAATTTAC AGTCATTTAT GCTGAGAAAC 
GGTATAATCG ATATAATTGA AAAGATGTTA ACAGATCCAT TGTTTAACTC AAAAAAAGCC 
TGGGACGATA ATGAAGATGA AAGAAGGATT GCATTGCAAG GCATACCTGT GCATGAAGTG 
AAGGCAAACT CTTTATGGGT ACTAAGGCAT CTAATGTACA ATTGCCAGAA TGAAGAGAAG 
TTTCAGCTTT TGGCCAAAAT CCCGATGAAT TTAATTTTGG ATTTTATTAA TGATCCATGT 
TGGGCAGTAC AAGCCCAATG TTTTCAGTTG CTCAGAAATT TGACTTGTAA TTCAAGGAAA 
ATTGTAAATA TTCTTTTGGA AAAATTTAAA GATGTAGAAT ACAAGATCGA TCCTCAAACA 
GGTAATAAAA TTAGCATCGG CTCAACGTAT CTTTTCGAAT TTCTTGCAAA GAAGATGAGG 
CTTTTAAATC CATTAGACAC TCAACAAAAG AAAGCAATGG AAGGCATCCT ATATATTATC 
GTGAATCTAG CGGCAGTCAA TGAGAATAAA AAGCAGCTAG TCATCGAACA AGACGAAATA 
TTGAACATTA TGAGTGAAAT ATTGGTAGAA ACAACGACTG ATAGTTCAAG TAATGGTAAT 
GACAGCAATC TGAAATTGGC ATGCCTCTGG GTACTCAATA ACTTACTTTG GAATTCATCA 
GTTTCACATT ATACCCAATA TGCAATTGAA AATGGTCTGG AACCAGGGCA TTCACCAAGT 
GATTCAGAAA ACCCTCAATC TACGGTGACA ATCGGGTATA ATGAATCAGT GGCTGGTGGA 
TATTCTAGGG GGAAATACTA CGATGAGCCA GATGGCGATG ATAGCAGTAG CAATGCGAAT 
GATGATGAAG ATGACGATAA TGATGAGGGT GATGATGAAG GCGATGAGTT CGTTCGCACC 
CCGGCTGCAA AAGGTAGTAC ATCAAATGTA CAAGTAACAC GTGCCACGGT GGAAAGATGT 
AGAAAACTCG TGGAGGTAGG ATTATACGAT CTGGTTAGAA AAAATATTAC AGACGAATCA 
TTATCTGTCA GGGAAAAGGC CAGAACGTTA CTATATCACA TGGACCTGCT TTTGAAAGTT 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012247.3
CDS	1..2766
Translation	MTVAYSLENLKKISNSLVGDQLAKVDYFLAPKCQIFQCLLSIEQSDGVELKNAKLDLLYTLLHLEPQQRDIVGTYYFDIVSAIYKSMSLASSFTKNNSSTNYKYIKLLNLCAGVYPNCGFPDLQYLQNGFIQLVNHKFLRSKCKIDEVVTIIELLKLFLLVDEKNCSDFNKSKFMEEEREVTETSHYQDFKMAESLEHIIVKISSKYLDQISLKYIVRLKVSRPASPSSVKNDPFDNKGVDCTRAIPKKINISNMYDSSLLSLALLLYLRYHYMIPGDRKLRNDATFKMFVLGLLKSNDVNIRCVALKFLLQPYFTEDKKWEDTRTLEKILPYLVKSFNYDPLPWWFDPFDMLDSLIVLYNEITPMNNPVLTTLAHTNVIFCILSRFAQCLSLPQHNEATLKTTTKFIKICASFAASDEKYRLLLLNDTLLLNHLEYGLESHITLIQDFISLKDEIKETTTESHSMCLPPIYDHDFVAAWLLLLKSFSRSVSALRTTLKRNKIAQLLLQILSKTYTLTKECYFAGQDFMKPEIMIMGITLGSICNFVVEFSNLQSFMLRNGIIDIIEKMLTDPLFNSKKAWDDNEDERRIALQGIPVHEVKANSLWVLRHLMYNCQNEEKFQLLAKIPMNLILDFINDPCWAVQAQCFQLLRNLTCNSRKIVNILLEKFKDVEYKIDPQTGNKISIGSTYLFEFLAKKMRLLNPLDTQQKKAMEGILYIIVNLAAVNENKKQLVIEQDEILNIMSEILVETTTDSSSNGNDSNLKLACLWVLNNLLWNSSVSHYTQYAIENGLEPGHSPSDSENPQSTVTIGYNESVAGGYSRGKYYDEPDGDDSSSNANDDEDDDNDEGDDEGDEFVRTPAAKGSTSNVQVTRATVERCRKLVEVGLYDLVRKNITDESLSVREKARTLLYHMDLLLKVK

Target ORF information:

RefSeq Version	NM_001179367.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID28 (VID28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179367.3

ORF Insert Sequence:

ATGACGGTGG CTTATTCCCT AGAGAATCTC AAAAAGATTA GTAATTCCCT GGTGGGAGAC 
CAGTTGGCTA AGGTAGATTA CTTTTTGGCT CCAAAGTGCC AGATTTTTCA ATGTTTGTTG 
TCCATTGAGC AAAGTGACGG TGTAGAGTTA AAAAATGCGA AATTAGATCT GCTATATACC 
CTTTTACATC TAGAACCGCA ACAAAGGGAT ATAGTAGGCA CATATTACTT TGATATTGTG 
TCCGCAATCT ATAAGTCCAT GAGTTTGGCA AGCAGTTTTA CTAAAAATAA TTCTTCGACA 
AACTATAAGT ACATAAAGCT TCTAAATTTG TGTGCAGGAG TTTATCCAAA TTGCGGATTC 
CCAGACTTGC AATACCTCCA GAACGGGTTC ATCCAGTTAG TCAACCATAA ATTTTTGAGG 
AGCAAATGTA AAATTGATGA GGTGGTCACG ATTATTGAAT TATTGAAGTT GTTTTTACTT 
GTGGACGAGA AAAATTGCAG CGACTTCAAC AAGAGCAAGT TTATGGAGGA AGAGAGAGAA 
GTGACAGAAA CTTCACACTA CCAAGATTTT AAAATGGCAG AATCCCTTGA ACACATAATA 
GTTAAAATAT CATCGAAGTA CTTGGACCAA ATAAGCCTCA AGTATATAGT GAGGCTAAAA 
GTATCCAGGC CAGCGTCACC ATCCAGCGTC AAGAACGATC CGTTTGACAA TAAAGGGGTC 
GACTGCACCC GTGCCATACC TAAAAAAATC AATATCTCAA ACATGTATGA TTCTAGTTTA 
CTCTCATTGG CACTCCTTCT ATATCTAAGA TATCATTATA TGATACCGGG TGATAGGAAA 
CTGCGGAATG ATGCGACGTT CAAGATGTTC GTTCTGGGTC TTTTGAAGAG TAATGACGTT 
AACATTAGGT GCGTGGCCCT GAAGTTTTTG TTACAACCGT ACTTTACAGA GGATAAGAAA 
TGGGAAGATA CGCGTACACT CGAGAAAATA CTACCCTATT TAGTTAAGTC ATTTAATTAT 
GACCCGTTGC CTTGGTGGTT TGATCCATTC GATATGCTGG ATTCATTGAT TGTGTTGTAT 
AATGAAATTA CTCCAATGAA TAATCCTGTG CTCACCACGC TAGCTCATAC AAACGTTATA 
TTTTGCATTC TATCCCGTTT TGCGCAGTGT CTTTCTCTAC CACAACACAA TGAAGCTACT 
TTGAAAACAA CTACAAAATT CATTAAAATA TGTGCGTCAT TTGCAGCATC GGACGAAAAA 
TACCGTCTTT TACTGTTGAA TGACACATTG TTGTTGAATC ATTTAGAATA CGGGTTGGAA 
TCCCACATTA CTTTGATACA GGATTTTATA TCTTTGAAAG ATGAGATAAA GGAAACAACT 
ACGGAAAGTC ATTCTATGTG TTTACCCCCA ATATATGATC ACGATTTCGT GGCAGCTTGG 
TTACTATTGT TGAAATCATT CTCGAGAAGT GTTTCAGCGC TTAGGACGAC GTTGAAGAGA 
AATAAAATTG CACAGCTGCT TTTACAGATA CTAAGTAAAA CATATACGTT GACCAAGGAA 
TGCTATTTTG CTGGACAAGA TTTTATGAAA CCGGAAATTA TGATTATGGG CATTACTCTG 
GGTAGTATTT GTAACTTCGT GGTGGAGTTC TCTAATTTAC AGTCATTTAT GCTGAGAAAC 
GGTATAATCG ATATAATTGA AAAGATGTTA ACAGATCCAT TGTTTAACTC AAAAAAAGCC 
TGGGACGATA ATGAAGATGA AAGAAGGATT GCATTGCAAG GCATACCTGT GCATGAAGTG 
AAGGCAAACT CTTTATGGGT ACTAAGGCAT CTAATGTACA ATTGCCAGAA TGAAGAGAAG 
TTTCAGCTTT TGGCCAAAAT CCCGATGAAT TTAATTTTGG ATTTTATTAA TGATCCATGT 
TGGGCAGTAC AAGCCCAATG TTTTCAGTTG CTCAGAAATT TGACTTGTAA TTCAAGGAAA 
ATTGTAAATA TTCTTTTGGA AAAATTTAAA GATGTAGAAT ACAAGATCGA TCCTCAAACA 
GGTAATAAAA TTAGCATCGG CTCAACGTAT CTTTTCGAAT TTCTTGCAAA GAAGATGAGG 
CTTTTAAATC CATTAGACAC TCAACAAAAG AAAGCAATGG AAGGCATCCT ATATATTATC 
GTGAATCTAG CGGCAGTCAA TGAGAATAAA AAGCAGCTAG TCATCGAACA AGACGAAATA 
TTGAACATTA TGAGTGAAAT ATTGGTAGAA ACAACGACTG ATAGTTCAAG TAATGGTAAT 
GACAGCAATC TGAAATTGGC ATGCCTCTGG GTACTCAATA ACTTACTTTG GAATTCATCA 
GTTTCACATT ATACCCAATA TGCAATTGAA AATGGTCTGG AACCAGGGCA TTCACCAAGT 
GATTCAGAAA ACCCTCAATC TACGGTGACA ATCGGGTATA ATGAATCAGT GGCTGGTGGA 
TATTCTAGGG GGAAATACTA CGATGAGCCA GATGGCGATG ATAGCAGTAG CAATGCGAAT 
GATGATGAAG ATGACGATAA TGATGAGGGT GATGATGAAG GCGATGAGTT CGTTCGCACC 
CCGGCTGCAA AAGGTAGTAC ATCAAATGTA CAAGTAACAC GTGCCACGGT GGAAAGATGT 
AGAAAACTCG TGGAGGTAGG ATTATACGAT CTGGTTAGAA AAAATATTAC AGACGAATCA 
TTATCTGTCA GGGAAAAGGC CAGAACGTTA CTATATCACA TGGACCTGCT TTTGAAAGTT 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VID28 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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