SEC6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC6
Entrez Gene ID	854742
Full Name	SNARE-binding exocyst subunit SEC6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179418.1	NP_012196.1	SNARE-binding exocyst subunit SEC6

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SEC6	NP_012196.1
Eremothecium gossypii	AGOS_ACL047W	NP_983357.2
Schizosaccharomyces pombe (fission yeast)	sec6	NP_587736.2
Arabidopsis thaliana (thale cress)	SEC6	NP_565026.1
Kluyveromyces lactis	KLLA0C07843g	XP_452549.1
Magnaporthe oryzae (rice blast fungus)	MGG_03235	XP_003716741.1
Neurospora crassa	NCU03341	XP_956450.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest a new model in which Sec6 promotes SNARE complex assembly, similar to the role proposed for other tether subunit-SNARE interactions
Title: The Exocyst Subunit Sec6 Interacts with Assembled Exocytic SNARE Complexes.

synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p
Title: The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p.

upon vesicle arrival, Sec6 is proposed to release Sec9 in favor of Sec6-exocyst assembly and to simultaneously recruit Sec1 to sites of secretion for coordinated SNARE complex formation and membrane fusion.
Title: Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1.

Sec6p is required to anchor exocyst complexes at sites of secretion.
Title: Sec6p anchors the assembled exocyst complex at sites of secretion.

Study shows that the single point mutation L633P in the SEC6 coding region defines the sec6-4 mutation phenotype.
Title: Characterization of the Saccharomyces cerevisiae sec6-4 mutation and tools to create S. cerevisiae strains containing the sec6-4 allele.

The following SEC6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03770	NM_001179418.1 Latest version!	Saccharomyces cerevisiae S288C SNARE-binding exocyst subunit SEC6 (SEC6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC6 ( NM_001179418.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012196.1 Saccharomyces cerevisiae S288C SNARE-binding exocyst subunit SEC6 (SEC6), partial mRNA.

Price & Availability↑

CloneID	OSi03770
Clone ID Related Accession (Same CDS sequence)	NM_001179418.1
Accession Version	NM_001179418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SNARE-binding exocyst subunit SEC6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTTCAG ATCCCTTGCA GCAAGTTTGT GATTTAATAA AGGGAGATCT ATCACTTGAA 
AGGGTACGAG ATATTAAGGA ACAGTTGCTA AAAGAAAAAT CCGTTGTAGA ATACCAATTG 
AATAAAGAAT CAGACAAATA CTATGGCGAA GTCGAAGAAT CCCTCAAACT ACTGAATCTG 
TCCAAAAATT CGGTCACTTC AATCAAACAA CAAATCAATG AAGTTAATAA ATTGGGTAAT 
GATAATCGAT TTGCAATTAA TCGTTATGAT ATACTTTTCC GAGCCACAAA ACTTTATGAA 
ACGGTCAATA CGACATCATC GATTTATGAT CGAATATATA ACTTTGTTGC ACTAATGGAG 
CATATAGAAA GACTATTAGT GGCGGAATTA GCGGAAGATG CCCTGGAAAC TGGTTGTCCC 
CATCTTTTGG AAATTCATTT CCTATTAACT TCAGCACGCG ACTTTCAAGA GCAGGTTGTT 
GTTATGGCTA AAGAAGCAAC TGAAGATGCA CAACGTACAG TCATGAAATT GTTTTCAAGA 
TTATCCGGAA TAATATCAAA ATTTGATAAA TTGCTGGACG GCTTAACTTA CGATATTGTA 
GAAATGGCCA GAGCTGAGCA AATATCTTTA GCAATTCGAC TTTTTAAAAT TTATGACCTA 
GAAGAAAGGG AAGATTTGAG AATTGAAGCT ATCAGAAATA TAATCAAGAA GAAAGAAATT 
GAAATAGAAA AATCCTCAAT TAAAAAACTT CCAAATAGCA AAAACACCGC AAGGTTACAA 
GATGAGACAC CCAAAGTTAT AGAATATCCG ACAAACAAGG GATTATACCA AGAAATAATG 
AGCGGTACCA TCTCAACAAG AACAGCACCT CGAGGTTACA AGCATTTTCT AATAAATGGT 
ATAAATAATT CCATTTCTGA AATGTTTGGT GAAATGAGAG AAAAATACGT TGGTGATCAA 
AAATTTGATG TTCTTGATAA CATGGATTGG ATTTTCAACG AATTGATTAT TGTAAAGGAA 
CATATTGCCA ACTGCTGTCC ACCACATTGG AACATATTTG AAGTATACTT TGACCAATAC 
TATAAGGAAT TGCATTCATT AATTACGGAT CTCGTGGAGT CCGAACCCGA GACCATTATT 
ATTCTTGATA TTCTTGCATT CGATAAGACG TTTCAAGATA CACTTAAGCA AGATTTTGGA 
TTTACCAAAA GCGAAGTCAA ATCTGTCATC GGCGATAAAG AAAAGGAAAC ACTATTTAAA 
GACTATTTGA ATTTGATAGT GGTTAAAATG ACCGAGTGGA TAGGTAATTT AGAAAAAGCA 
GAATTTGATG TATTCTTGGA AAGAAGTACC CCGCCACATT CTGATTCAGA TGGATTGCTT 
TTTCTTGATG GTACTAAGAC TTGTTTTCAA ATGTTTACAC AGCAAGTGGA AGTGGCTGCA 
GGTACTAATC AGGCCAAAAT ACTTGTGGGT GTGGTTGAAA GGTTCAGTGA TCTACTAACA 
AAGAGGCAAA AAAACTGGAT TTCTAAAATA AGTGAAGAAA TCAAAAAGCA AATCAATTAC 
AATCACAAGT ATGATATAGA TCCAGAGAGT ATAACTCCTG AAGATGAGTG TCCAGGAGGT 
TTGGTGGAAT ACTTAATTGC TGTATCAAAC GACCAAATGA AAGCTGCGGA CTATGCCGTT 
GCCATTTCAT CCAAGTATGG TAAACTGGTT TCTAAAGTTT ATGAGAAGCA AATTACAAAT 
CACTTGGAGG GCACTTTAGA TGGTTTTGCT GAAGTTGCTC AGTGCAGTTC TTTAGGCCTA 
ATCACATTGA TGTTCGATGA TCTGAGAAAA CCCTACCAAG AGATTTTTAG CAAGACATGG 
TATATGGGGA GCCAAGCGCA GCAAATTGCG GACACACTAG ATGAATATCT TTTGGATATC 
AAACCTCAGA TGAATTCTGT GCTGTTCGTG AACTTCATAG ACAATGTTAT TGGTGAGACA 
ATTATAAAAT TTTTGACTGC TTTATCGTTC GAGCATTCAT TCAAAAACAA AAACAATAAG 
TTTTTAGAGG CGATGAAGAG GGATTTTGAA ATTTTTTATC AGCTCTTTGT CAAGGTGCTG 
GATGGTAATG AAAGCAAGGA TACGCTAATT ACACAAAATT TTACTGTAAT GGAGTTCTTC 
ATGGACCTAA GCTGTGAGCC GATAGATAGC ATATTAGATA TTTGGCAAAA GTATCTTGAA 
GTATACTGGG ACTCGCGCAT AGATTTACTG GTAGGAATAT TGAAGTGTAG GAAAGATGTG 
AGTAGCTCAG AAAGAAAGAA GATCGTACAA CAGGCTACGG AAATGTTACA TGAATACCGC 
CGTAACATGG AGGCCAACGG TGTGGATCGC GAACCTACTT TGATGAGGCG GTTTGTATTA 
GAATTCGAAA AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012196.1
CDS	1..2418
Translation	MSSDPLQQVCDLIKGDLSLERVRDIKEQLLKEKSVVEYQLNKESDKYYGEVEESLKLLNLSKNSVTSIKQQINEVNKLGNDNRFAINRYDILFRATKLYETVNTTSSIYDRIYNFVALMEHIERLLVAELAEDALETGCPHLLEIHFLLTSARDFQEQVVVMAKEATEDAQRTVMKLFSRLSGIISKFDKLLDGLTYDIVEMARAEQISLAIRLFKIYDLEEREDLRIEAIRNIIKKKEIEIEKSSIKKLPNSKNTARLQDETPKVIEYPTNKGLYQEIMSGTISTRTAPRGYKHFLINGINNSISEMFGEMREKYVGDQKFDVLDNMDWIFNELIIVKEHIANCCPPHWNIFEVYFDQYYKELHSLITDLVESEPETIIILDILAFDKTFQDTLKQDFGFTKSEVKSVIGDKEKETLFKDYLNLIVVKMTEWIGNLEKAEFDVFLERSTPPHSDSDGLLFLDGTKTCFQMFTQQVEVAAGTNQAKILVGVVERFSDLLTKRQKNWISKISEEIKKQINYNHKYDIDPESITPEDECPGGLVEYLIAVSNDQMKAADYAVAISSKYGKLVSKVYEKQITNHLEGTLDGFAEVAQCSSLGLITLMFDDLRKPYQEIFSKTWYMGSQAQQIADTLDEYLLDIKPQMNSVLFVNFIDNVIGETIIKFLTALSFEHSFKNKNNKFLEAMKRDFEIFYQLFVKVLDGNESKDTLITQNFTVMEFFMDLSCEPIDSILDIWQKYLEVYWDSRIDLLVGILKCRKDVSSSERKKIVQQATEMLHEYRRNMEANGVDREPTLMRRFVLEFEKQ

Target ORF information:

RefSeq Version	NM_001179418.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SNARE-binding exocyst subunit SEC6 (SEC6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179418.1

ORF Insert Sequence:

ATGTCTTCAG ATCCCTTGCA GCAAGTTTGT GATTTAATAA AGGGAGATCT ATCACTTGAA 
AGGGTACGAG ATATTAAGGA ACAGTTGCTA AAAGAAAAAT CCGTTGTAGA ATACCAATTG 
AATAAAGAAT CAGACAAATA CTATGGCGAA GTCGAAGAAT CCCTCAAACT ACTGAATCTG 
TCCAAAAATT CGGTCACTTC AATCAAACAA CAAATCAATG AAGTTAATAA ATTGGGTAAT 
GATAATCGAT TTGCAATTAA TCGTTATGAT ATACTTTTCC GAGCCACAAA ACTTTATGAA 
ACGGTCAATA CGACATCATC GATTTATGAT CGAATATATA ACTTTGTTGC ACTAATGGAG 
CATATAGAAA GACTATTAGT GGCGGAATTA GCGGAAGATG CCCTGGAAAC TGGTTGTCCC 
CATCTTTTGG AAATTCATTT CCTATTAACT TCAGCACGCG ACTTTCAAGA GCAGGTTGTT 
GTTATGGCTA AAGAAGCAAC TGAAGATGCA CAACGTACAG TCATGAAATT GTTTTCAAGA 
TTATCCGGAA TAATATCAAA ATTTGATAAA TTGCTGGACG GCTTAACTTA CGATATTGTA 
GAAATGGCCA GAGCTGAGCA AATATCTTTA GCAATTCGAC TTTTTAAAAT TTATGACCTA 
GAAGAAAGGG AAGATTTGAG AATTGAAGCT ATCAGAAATA TAATCAAGAA GAAAGAAATT 
GAAATAGAAA AATCCTCAAT TAAAAAACTT CCAAATAGCA AAAACACCGC AAGGTTACAA 
GATGAGACAC CCAAAGTTAT AGAATATCCG ACAAACAAGG GATTATACCA AGAAATAATG 
AGCGGTACCA TCTCAACAAG AACAGCACCT CGAGGTTACA AGCATTTTCT AATAAATGGT 
ATAAATAATT CCATTTCTGA AATGTTTGGT GAAATGAGAG AAAAATACGT TGGTGATCAA 
AAATTTGATG TTCTTGATAA CATGGATTGG ATTTTCAACG AATTGATTAT TGTAAAGGAA 
CATATTGCCA ACTGCTGTCC ACCACATTGG AACATATTTG AAGTATACTT TGACCAATAC 
TATAAGGAAT TGCATTCATT AATTACGGAT CTCGTGGAGT CCGAACCCGA GACCATTATT 
ATTCTTGATA TTCTTGCATT CGATAAGACG TTTCAAGATA CACTTAAGCA AGATTTTGGA 
TTTACCAAAA GCGAAGTCAA ATCTGTCATC GGCGATAAAG AAAAGGAAAC ACTATTTAAA 
GACTATTTGA ATTTGATAGT GGTTAAAATG ACCGAGTGGA TAGGTAATTT AGAAAAAGCA 
GAATTTGATG TATTCTTGGA AAGAAGTACC CCGCCACATT CTGATTCAGA TGGATTGCTT 
TTTCTTGATG GTACTAAGAC TTGTTTTCAA ATGTTTACAC AGCAAGTGGA AGTGGCTGCA 
GGTACTAATC AGGCCAAAAT ACTTGTGGGT GTGGTTGAAA GGTTCAGTGA TCTACTAACA 
AAGAGGCAAA AAAACTGGAT TTCTAAAATA AGTGAAGAAA TCAAAAAGCA AATCAATTAC 
AATCACAAGT ATGATATAGA TCCAGAGAGT ATAACTCCTG AAGATGAGTG TCCAGGAGGT 
TTGGTGGAAT ACTTAATTGC TGTATCAAAC GACCAAATGA AAGCTGCGGA CTATGCCGTT 
GCCATTTCAT CCAAGTATGG TAAACTGGTT TCTAAAGTTT ATGAGAAGCA AATTACAAAT 
CACTTGGAGG GCACTTTAGA TGGTTTTGCT GAAGTTGCTC AGTGCAGTTC TTTAGGCCTA 
ATCACATTGA TGTTCGATGA TCTGAGAAAA CCCTACCAAG AGATTTTTAG CAAGACATGG 
TATATGGGGA GCCAAGCGCA GCAAATTGCG GACACACTAG ATGAATATCT TTTGGATATC 
AAACCTCAGA TGAATTCTGT GCTGTTCGTG AACTTCATAG ACAATGTTAT TGGTGAGACA 
ATTATAAAAT TTTTGACTGC TTTATCGTTC GAGCATTCAT TCAAAAACAA AAACAATAAG 
TTTTTAGAGG CGATGAAGAG GGATTTTGAA ATTTTTTATC AGCTCTTTGT CAAGGTGCTG 
GATGGTAATG AAAGCAAGGA TACGCTAATT ACACAAAATT TTACTGTAAT GGAGTTCTTC 
ATGGACCTAA GCTGTGAGCC GATAGATAGC ATATTAGATA TTTGGCAAAA GTATCTTGAA 
GTATACTGGG ACTCGCGCAT AGATTTACTG GTAGGAATAT TGAAGTGTAG GAAAGATGTG 
AGTAGCTCAG AAAGAAAGAA GATCGTACAA CAGGCTACGG AAATGTTACA TGAATACCGC 
CGTAACATGG AGGCCAACGG TGTGGATCGC GAACCTACTT TGATGAGGCG GTTTGTATTA 
GAATTCGAAA AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEC6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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