SPO22 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SPO22
Entrez Gene ID	854737
Full Name	Spo22p
Synonyms	ZIP4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179423.1	NP_012192.2	Spo22p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SPO22	NP_012192.2
Kluyveromyces lactis	KLLA0C09328g	XP_452615.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ZIP4 was not required for centromere pairing during pachytene, but was required for proper segregation of achiasmate chromosomes.
Title: Meiotic Centromere Coupling and Pairing Function by Two Separate Mechanisms in Saccharomyces cerevisiae.

The excess crossovers observed in synaptonemal complex (SC), central element-deficient mutants are Msh4, Zip1, and Zip4 dependent, clearly indicating the existence of two classes of SC proteins-a class with procrossover function(s) that are also necessary for SC assembly and a class that is not required for crossover formation but essential for SC assembly
Title: Synaptonemal Complex Proteins of Budding Yeast Define Reciprocal Roles in MutS?-Mediated Crossover Formation.

Interference and assurance in genetic crossovers are mechanistically distinct processes that are separated by mutations in a new ZMM protein from Saccharomyces cerevisiae, Spo16.
Title: Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosis.

Spo22 (also called Zip4) is identified as a probable functional collaborator of Zip2/3
Title: Bioinformatic analyses implicate the collaborating meiotic crossover/chiasma proteins Zip2, Zip3, and Spo22/Zip4 in ubiquitin labeling.

The following SPO22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPO22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03765	NM_001179423.1 Latest version!	Saccharomyces cerevisiae S288C Spo22p (SPO22), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPO22 ( NM_001179423.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012192.2 Saccharomyces cerevisiae S288C Spo22p (SPO22), partial mRNA.

Price & Availability↑

CloneID	OSi03765
Clone ID Related Accession (Same CDS sequence)	NM_001179423.1
Accession Version	NM_001179423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Spo22p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGACC ACAACGTTAA CTCCACTTTT AGAAAAACTT TGGTCGAGTT ATGCGAAACT 
GCAACTTGGA TAACATCGCA AGTTTATGCG GCTAAGAATT TAGAGAAAAA CGATTTGATT 
ACAGTAGATA ATAAAATAAG CGCATTGTAT CCCATTGCAG AAAAATATGA TAGATCATTC 
AGAACCACTA CAGTTATATT AGACGAAGAA CTTATCCTGA AATTGGAAAA TGCAGCATCA 
AGTCTATGGA ATTCGTTGAC TATTGCTATG AAAGCTGAAA AAGCTTCAGA CAAATATTTT 
AATGAAGTCT TTTGCAAATG TAAGATTTTC GCAACCAAGC TTCTCTCCAT CCATGAGGCT 
TTGTTTCGGA CAAACACCAA CCTCCTACGG AACTTCAAAT GTTATATCAG TTCTTTCAAA 
AGCGCTAGTG AGTATCGTTT TGACGATTTA ATAACTAATA CACAGCAGCA TTCAGAGAAA 
TACTTACAGA TAATCAATGA GAATGTGGAA AGTTTCTCCA ATGAAGAGAA AACTGAGTTT 
AAAAAACTGA CGTTCGAGTT TTACTTGGTA AATTTTCAAT TATATCTTTC GGAAAATGAC 
CTGGATACAG CAAATATTTA CACTGCAAAA GTAAACATCA CTGATAATTC CAAATATATG 
GACGCAGATT TGCTCATCGA ATTATGCAGA ATGATCTATA ATTCCACAGT TATGTTGAAA 
GAAATTAATA ATCCTGAAAC TCAGTTAGTA GACGTAAATA TAATTTCTTT TTTGAAGGAT 
GTGGAAAAAT ATTTGGAGTT ACCTGTGGAA AATTTAAAAT CTCATACTGA TTATAGTAAT 
TTAAAGTATT CAGTTTTGAT TTTTATGGCA AATTGTTTGG TTGAAGGACA CCCACAAGCT 
TCTGAACTTG AACAATGTGA TCACTATTTA AGTCTTTTGC AAAATGAATA TCCAAACAAA 
GTGGACCCTT TTATATTGGC TATCAATTTA ACTAAACGGA GAAATATCGT AAATCCGGCA 
GAAACCATAG AGGAAATTCT CATGCGAATG ATTATGTCAG TAGATGTAAT ATCCAATTTT 
CAAGCTGTCA TAGCTTCTAT CAATGACTTA TCAAAAATGA ACACCAAATT CTCTATAGTG 
TGTCTCGATT ATTTGTTAAT CAATAAACTG AATTCTAAGA ATGATAGTAA ATTCTTAGGA 
AAAGCCATTT GCTCCAGGTT TTTAATTACA ACACAATCAA AAACAATGAA TGATTCCGAG 
ATAGCGGAAA GCTTAGAGAA TTTTAGCACA CAGATGGAAA GAATAGTTTC AGAACCCCTC 
ACCAAACATG CTATCTCTTG CATAATAACT TTATTATGGA ATACTGGAAA AAAGTTAGAA 
AAGATGGAAA AATACGTTGT TAGTATTAGA TTTTACAAAT TAGCACTCAA AGATATTATC 
AGTCAAAATT ATTCCGACCG GGGGAAAATT CAAAGGGCTC TACAAGTCGT ATATAACAAA 
ATTGAAGACT ATTCCAACAC AGTTAGAGTA TATCAGGATA TGGACGAAGT AGATAGACAA 
TCTCCTCTGT GTCAACTTTT GATGTTGCAG TCTTTTCTAG CGGATGATAA AACAGAGGAA 
GCGCTTACGT GCCTCCAGAA GATAAAGTCG TCTGAAGATG AAAAAAGTAC AGACGCTCTT 
ATATTAGCAG TAGCAGAATG CAAACGAAAG ACAGATTTGT CTGTTCAAGG GCTCTTAATG 
ATATTTGACA AATTACAATC TAAAAGCAAT TCGCAAACTA TATCATCTAC GTCGAGTTCT 
CAGACACTGA GCATTCTCAG GTATACCCTA CAGATGATTG TCAAAGTATC GGAAGAGGAG 
CCTTTAGAAA CCTTCATAAA TTATTTACCA ACAGTACAGA AACTTTTACA GAAGGCTGTC 
GAGTTTCTCA AAACTGTAAA GTTACTAAAT CAATTACCAC CAGACGTTGA GAAGGAAGCA 
ATATATCAGC AATCAGTGGC TGTAAACGAA ATCGAGTGGT TTGCCTCTTT TTCCTATAAT 
GTGGCTGTAA AATGCTTAGT TGATCAGTCC TGTGAATCCA TCTCCGAGTT TCCCCAGTAT 
TGCATTCAAT TTATTGACCT TATTCCGGTC CAAGACTTCA CTTTCCCGAA AATGTATCAT 
TTCACATATT GGAGGTTCAA AGCCACAATC TTGCAGTTGA TAATAGCCAA AGAAAAAGCA 
AAGCAAGACC AACACCAAAA GGATTGGGAT ATTTATGAGA AGTCAGAAGA GTTGGTTAAT 
AGCATAAATG TTATGAAGAA AAGCTCGGAA TTTAAGGATG GGTCATCATT GGAAGATAGA 
AATACATTAC ATGAATGTTT CCTGGAGGCG TTGACTATAC ATCTGGAAAG TGCCTTAATG 
ATGCCTGATC AAACTAGGAT TCTGGACATA TTGAAGAAAA CGGAATTGTA TCAAGATAGC 
CGCGTGGATG CGCTACTTAT TGATATCTCG TCGAATATGG AAGATTTACC TAAAGGAGTT 
CTCATAGAGA TACTTGAAAC AGTATTAAAA AGAAACATGG GCCCTGAAGT GAAAGAACGG 
GAACTCTGTA GCTGGCTGCG AATTTTACTG GAAAATGCAA TTAATCTTAA CCATGAGGTC 
GAACTACGCA TATTGGATAG GGTTCTTAAG ATCCTAAATA TAAATCAATC AAGTCTTCAA 
GACACAGATG GTGTCCTTCA AACAGAGTTA GAAACTATAG CCACTTACTG TTGGAATATC 
GGCGTCAATT ACATAATCAA AGACAACAAA AGCAATGGAA TTGTTTGGTG TAAACATTCA 
ATGGGGTTCG CAAACATGGT TAATGAAGGC TTGCAGGAGC AATTGTACTC TTTATGGGAG 
TCTTTAGCAA GTTCGGCAAA TATCGATATC AATAGTATTG CTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012192.2
CDS	1..2928
Translation	MSDHNVNSTFRKTLVELCETATWITSQVYAAKNLEKNDLITVDNKISALYPIAEKYDRSFRTTTVILDEELILKLENAASSLWNSLTIAMKAEKASDKYFNEVFCKCKIFATKLLSIHEALFRTNTNLLRNFKCYISSFKSASEYRFDDLITNTQQHSEKYLQIINENVESFSNEEKTEFKKLTFEFYLVNFQLYLSENDLDTANIYTAKVNITDNSKYMDADLLIELCRMIYNSTVMLKEINNPETQLVDVNIISFLKDVEKYLELPVENLKSHTDYSNLKYSVLIFMANCLVEGHPQASELEQCDHYLSLLQNEYPNKVDPFILAINLTKRRNIVNPAETIEEILMRMIMSVDVISNFQAVIASINDLSKMNTKFSIVCLDYLLINKLNSKNDSKFLGKAICSRFLITTQSKTMNDSEIAESLENFSTQMERIVSEPLTKHAISCIITLLWNTGKKLEKMEKYVVSIRFYKLALKDIISQNYSDRGKIQRALQVVYNKIEDYSNTVRVYQDMDEVDRQSPLCQLLMLQSFLADDKTEEALTCLQKIKSSEDEKSTDALILAVAECKRKTDLSVQGLLMIFDKLQSKSNSQTISSTSSSQTLSILRYTLQMIVKVSEEEPLETFINYLPTVQKLLQKAVEFLKTVKLLNQLPPDVEKEAIYQQSVAVNEIEWFASFSYNVAVKCLVDQSCESISEFPQYCIQFIDLIPVQDFTFPKMYHFTYWRFKATILQLIIAKEKAKQDQHQKDWDIYEKSEELVNSINVMKKSSEFKDGSSLEDRNTLHECFLEALTIHLESALMMPDQTRILDILKKTELYQDSRVDALLIDISSNMEDLPKGVLIEILETVLKRNMGPEVKERELCSWLRILLENAINLNHEVELRILDRVLKILNINQSSLQDTDGVLQTELETIATYCWNIGVNYIIKDNKSNGIVWCKHSMGFANMVNEGLQEQLYSLWESLASSANIDINSIAK

Target ORF information:

RefSeq Version	NM_001179423.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Spo22p (SPO22), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179423.1

ORF Insert Sequence:

ATGTCAGACC ACAACGTTAA CTCCACTTTT AGAAAAACTT TGGTCGAGTT ATGCGAAACT 
GCAACTTGGA TAACATCGCA AGTTTATGCG GCTAAGAATT TAGAGAAAAA CGATTTGATT 
ACAGTAGATA ATAAAATAAG CGCATTGTAT CCCATTGCAG AAAAATATGA TAGATCATTC 
AGAACCACTA CAGTTATATT AGACGAAGAA CTTATCCTGA AATTGGAAAA TGCAGCATCA 
AGTCTATGGA ATTCGTTGAC TATTGCTATG AAAGCTGAAA AAGCTTCAGA CAAATATTTT 
AATGAAGTCT TTTGCAAATG TAAGATTTTC GCAACCAAGC TTCTCTCCAT CCATGAGGCT 
TTGTTTCGGA CAAACACCAA CCTCCTACGG AACTTCAAAT GTTATATCAG TTCTTTCAAA 
AGCGCTAGTG AGTATCGTTT TGACGATTTA ATAACTAATA CACAGCAGCA TTCAGAGAAA 
TACTTACAGA TAATCAATGA GAATGTGGAA AGTTTCTCCA ATGAAGAGAA AACTGAGTTT 
AAAAAACTGA CGTTCGAGTT TTACTTGGTA AATTTTCAAT TATATCTTTC GGAAAATGAC 
CTGGATACAG CAAATATTTA CACTGCAAAA GTAAACATCA CTGATAATTC CAAATATATG 
GACGCAGATT TGCTCATCGA ATTATGCAGA ATGATCTATA ATTCCACAGT TATGTTGAAA 
GAAATTAATA ATCCTGAAAC TCAGTTAGTA GACGTAAATA TAATTTCTTT TTTGAAGGAT 
GTGGAAAAAT ATTTGGAGTT ACCTGTGGAA AATTTAAAAT CTCATACTGA TTATAGTAAT 
TTAAAGTATT CAGTTTTGAT TTTTATGGCA AATTGTTTGG TTGAAGGACA CCCACAAGCT 
TCTGAACTTG AACAATGTGA TCACTATTTA AGTCTTTTGC AAAATGAATA TCCAAACAAA 
GTGGACCCTT TTATATTGGC TATCAATTTA ACTAAACGGA GAAATATCGT AAATCCGGCA 
GAAACCATAG AGGAAATTCT CATGCGAATG ATTATGTCAG TAGATGTAAT ATCCAATTTT 
CAAGCTGTCA TAGCTTCTAT CAATGACTTA TCAAAAATGA ACACCAAATT CTCTATAGTG 
TGTCTCGATT ATTTGTTAAT CAATAAACTG AATTCTAAGA ATGATAGTAA ATTCTTAGGA 
AAAGCCATTT GCTCCAGGTT TTTAATTACA ACACAATCAA AAACAATGAA TGATTCCGAG 
ATAGCGGAAA GCTTAGAGAA TTTTAGCACA CAGATGGAAA GAATAGTTTC AGAACCCCTC 
ACCAAACATG CTATCTCTTG CATAATAACT TTATTATGGA ATACTGGAAA AAAGTTAGAA 
AAGATGGAAA AATACGTTGT TAGTATTAGA TTTTACAAAT TAGCACTCAA AGATATTATC 
AGTCAAAATT ATTCCGACCG GGGGAAAATT CAAAGGGCTC TACAAGTCGT ATATAACAAA 
ATTGAAGACT ATTCCAACAC AGTTAGAGTA TATCAGGATA TGGACGAAGT AGATAGACAA 
TCTCCTCTGT GTCAACTTTT GATGTTGCAG TCTTTTCTAG CGGATGATAA AACAGAGGAA 
GCGCTTACGT GCCTCCAGAA GATAAAGTCG TCTGAAGATG AAAAAAGTAC AGACGCTCTT 
ATATTAGCAG TAGCAGAATG CAAACGAAAG ACAGATTTGT CTGTTCAAGG GCTCTTAATG 
ATATTTGACA AATTACAATC TAAAAGCAAT TCGCAAACTA TATCATCTAC GTCGAGTTCT 
CAGACACTGA GCATTCTCAG GTATACCCTA CAGATGATTG TCAAAGTATC GGAAGAGGAG 
CCTTTAGAAA CCTTCATAAA TTATTTACCA ACAGTACAGA AACTTTTACA GAAGGCTGTC 
GAGTTTCTCA AAACTGTAAA GTTACTAAAT CAATTACCAC CAGACGTTGA GAAGGAAGCA 
ATATATCAGC AATCAGTGGC TGTAAACGAA ATCGAGTGGT TTGCCTCTTT TTCCTATAAT 
GTGGCTGTAA AATGCTTAGT TGATCAGTCC TGTGAATCCA TCTCCGAGTT TCCCCAGTAT 
TGCATTCAAT TTATTGACCT TATTCCGGTC CAAGACTTCA CTTTCCCGAA AATGTATCAT 
TTCACATATT GGAGGTTCAA AGCCACAATC TTGCAGTTGA TAATAGCCAA AGAAAAAGCA 
AAGCAAGACC AACACCAAAA GGATTGGGAT ATTTATGAGA AGTCAGAAGA GTTGGTTAAT 
AGCATAAATG TTATGAAGAA AAGCTCGGAA TTTAAGGATG GGTCATCATT GGAAGATAGA 
AATACATTAC ATGAATGTTT CCTGGAGGCG TTGACTATAC ATCTGGAAAG TGCCTTAATG 
ATGCCTGATC AAACTAGGAT TCTGGACATA TTGAAGAAAA CGGAATTGTA TCAAGATAGC 
CGCGTGGATG CGCTACTTAT TGATATCTCG TCGAATATGG AAGATTTACC TAAAGGAGTT 
CTCATAGAGA TACTTGAAAC AGTATTAAAA AGAAACATGG GCCCTGAAGT GAAAGAACGG 
GAACTCTGTA GCTGGCTGCG AATTTTACTG GAAAATGCAA TTAATCTTAA CCATGAGGTC 
GAACTACGCA TATTGGATAG GGTTCTTAAG ATCCTAAATA TAAATCAATC AAGTCTTCAA 
GACACAGATG GTGTCCTTCA AACAGAGTTA GAAACTATAG CCACTTACTG TTGGAATATC 
GGCGTCAATT ACATAATCAA AGACAACAAA AGCAATGGAA TTGTTTGGTG TAAACATTCA 
ATGGGGTTCG CAAACATGGT TAATGAAGGC TTGCAGGAGC AATTGTACTC TTTATGGGAG 
TCTTTAGCAA GTTCGGCAAA TATCGATATC AATAGTATTG CTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SPO22 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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