ATG32 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ATG32
Entrez Gene ID	854660
Full Name	mitophagy protein ATG32
Synonyms	ECM37
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179494.1	NP_012120.1	mitophagy protein ATG32

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ATG32	NP_012120.1
Kluyveromyces lactis	KLLA0A00660g	XP_451027.1
Eremothecium gossypii	AGOS_AFR283W	NP_985830.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results reveal that a posttranslational modification discriminates a form of Atg32 targeting mitochondria for mitophagy from that, which escapes mitophagy by rapid degradation.
Title: The Degradation Pathway of the Mitophagy Receptor Atg32 Is Re-Routed by a Posttranslational Modification.

Amelioration of Atg32 expression and attenuation of Atg8-phosphatidylmonomethylethanolamine conjugation markedly rescue mitophagy in opi null cells.
Title: Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling.

Based on findings, authors conclude that the Tor and PpSin3-PpRpd3 pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation.
Title: Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast.

data implicate Atg32 as a bipartite platform recruiting Atg8 and Atg11 to the mitochondrial surface and forming an initiator complex crucial for mitophagy.
Title: Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy.

Phosphorylation of Serine 114 on Atg32 mediates mitophagy
Title: Phosphorylation of Serine 114 on Atg32 mediates mitophagy.

The following ATG32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03697	NM_001179494.1 Latest version!	Saccharomyces cerevisiae S288C mitophagy protein ATG32 (ATG32), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATG32 ( NM_001179494.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012120.1 Saccharomyces cerevisiae S288C mitophagy protein ATG32 (ATG32), partial mRNA.

Price & Availability↑

CloneID	OSi03697
Clone ID Related Accession (Same CDS sequence)	NM_001179494.1
Accession Version	NM_001179494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mitophagy protein ATG32
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTTTGG AATACCAACA AAGGGAAGGT AAAGGATCCT CATCTAAAAG CATGCCACCT 
GATTCCTCGT CAACGACTAT TCACACATGC TCTGAAGCAC AAACTGGGGA AGACAAAGGC 
TTACTTGACC CTCATCTATC AGTATTGGAA TTGTTGAGTA AGACAGGGCA TTCGCCTTCG 
CCAATGGGAC AGAACTTGGT TACGTCCATT GACATATCCG GCAACCACAA TGTAAACGAT 
AGTATATCAG GATCGTGGCA AGCCATTCAG CCTTTGGACT TGGGCGCTTC TTTCATCCCA 
GAAAGGTGCT CTTCTCAAAC GACTAATGGA AGTATTCTAT CATCTAGTGA TACTTCTGAA 
GAAGAACAAG AGCTGCTTCA AGCGCCTGCT GCTGATATAA TAAATATTAT TAAACAAGGA 
CAAGAAGGAG CAAATGTCGT TTCACCGTCT CATCCCTTTA AACAGTTACA GAAGATAATA 
TCGCTGCCCT TGCCTGGTAA GGAAAAAACT CCTTTTAACG AGCAAGATGA TGATGGTGAT 
GAAGATGAGG CTTTTGAGGA AGATAGTGTG ACGATAACAA AAAGCTTGAC TTCTTCGACA 
AACTCTTTTG TTATGCCCAA ACTATCACTA ACACAAAAGA ATCCAGTTTT TCGCCTTCTA 
ATACTGGGAA GGACTGGTTC CAGCTTTTAT CAATCAATAC CAAAAGAATA CCAATCTCTT 
TTTGAATTAC CCAAATATCA CGATTCCGCA ACATTCCCGC AATATACGGG GATTGTCATA 
ATTTTTCAGG AGCTGAGAGA GATGGTATCT CTGCTAAACA GGATCGTTCA ATATTCTCAA 
GGCAAGCCTG TAATACCGAT CTGCCAGCCT GGGCAAGTCA TCCAAGTGAA AAACGTCCTG 
AAGTCATTCT TAAGGAATAA ACTTGTTAAA TTATTGTTTC CTCCAGTTGT TGTGACCAAC 
AAAAGAGATT TGAAAAAAAT GTTTCAAAGA TTACAAGATT TATCCTTGGA ATACGGAGAA 
GATGTGAATG AGGAAGATAA TGATGACGAA GCTATACATA CAAAGTCTCG GTCCTATTGT 
CGTAACAAAA AAGCCGAAAA CTCAAAAAAG AAATCCCCTA AATCGAATAA AAAACCCAAG 
AGAAAGAAGC AGAAGTTCTT CACGAGCTGG TTCACTTGGG GCATTTCAAT TACTATAGGA 
ATTAGTTTTG GATGTTGTGT GACATACTTT GTCACTGCAG CATACGAACA CCAAACAGTT 
AAGTCTTTAA GTTTGCGGCC CTCCATATTG GCCTCTTTAC TTTCCTTAGA TTCCTCTAGC 
GACACAATCA ATACGCCCGC CACTGCATCT CCTTCTTCAA CTGAGCAGTT TTTATGGTTT 
GATAAGGGTA CGCTTCAGAT AAACTTCCAC TCCGATGGAT TCATTATGAA AAGTCTTACC 
ATAATCAAGG AGACTTGGGG CAAAATGAAT ACTTTTGTCT TGCATGCTCT TTCTAAACCT 
TTAAAATTTT TGGAGAACCT TAATAAAAGC TCTGAATTTT CCATTGATGA ATCTAATCGG 
ATTTTGGCAC TGGGTTATAT TCTATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012120.1
CDS	1..1590
Translation	MVLEYQQREGKGSSSKSMPPDSSSTTIHTCSEAQTGEDKGLLDPHLSVLELLSKTGHSPSPMGQNLVTSIDISGNHNVNDSISGSWQAIQPLDLGASFIPERCSSQTTNGSILSSSDTSEEEQELLQAPAADIINIIKQGQEGANVVSPSHPFKQLQKIISLPLPGKEKTPFNEQDDDGDEDEAFEEDSVTITKSLTSSTNSFVMPKLSLTQKNPVFRLLILGRTGSSFYQSIPKEYQSLFELPKYHDSATFPQYTGIVIIFQELREMVSLLNRIVQYSQGKPVIPICQPGQVIQVKNVLKSFLRNKLVKLLFPPVVVTNKRDLKKMFQRLQDLSLEYGEDVNEEDNDDEAIHTKSRSYCRNKKAENSKKKSPKSNKKPKRKKQKFFTSWFTWGISITIGISFGCCVTYFVTAAYEHQTVKSLSLRPSILASLLSLDSSSDTINTPATASPSSTEQFLWFDKGTLQINFHSDGFIMKSLTIIKETWGKMNTFVLHALSKPLKFLENLNKSSEFSIDESNRILALGYILL

Target ORF information:

RefSeq Version	NM_001179494.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mitophagy protein ATG32 (ATG32), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179494.1

ORF Insert Sequence:

ATGGTTTTGG AATACCAACA AAGGGAAGGT AAAGGATCCT CATCTAAAAG CATGCCACCT 
GATTCCTCGT CAACGACTAT TCACACATGC TCTGAAGCAC AAACTGGGGA AGACAAAGGC 
TTACTTGACC CTCATCTATC AGTATTGGAA TTGTTGAGTA AGACAGGGCA TTCGCCTTCG 
CCAATGGGAC AGAACTTGGT TACGTCCATT GACATATCCG GCAACCACAA TGTAAACGAT 
AGTATATCAG GATCGTGGCA AGCCATTCAG CCTTTGGACT TGGGCGCTTC TTTCATCCCA 
GAAAGGTGCT CTTCTCAAAC GACTAATGGA AGTATTCTAT CATCTAGTGA TACTTCTGAA 
GAAGAACAAG AGCTGCTTCA AGCGCCTGCT GCTGATATAA TAAATATTAT TAAACAAGGA 
CAAGAAGGAG CAAATGTCGT TTCACCGTCT CATCCCTTTA AACAGTTACA GAAGATAATA 
TCGCTGCCCT TGCCTGGTAA GGAAAAAACT CCTTTTAACG AGCAAGATGA TGATGGTGAT 
GAAGATGAGG CTTTTGAGGA AGATAGTGTG ACGATAACAA AAAGCTTGAC TTCTTCGACA 
AACTCTTTTG TTATGCCCAA ACTATCACTA ACACAAAAGA ATCCAGTTTT TCGCCTTCTA 
ATACTGGGAA GGACTGGTTC CAGCTTTTAT CAATCAATAC CAAAAGAATA CCAATCTCTT 
TTTGAATTAC CCAAATATCA CGATTCCGCA ACATTCCCGC AATATACGGG GATTGTCATA 
ATTTTTCAGG AGCTGAGAGA GATGGTATCT CTGCTAAACA GGATCGTTCA ATATTCTCAA 
GGCAAGCCTG TAATACCGAT CTGCCAGCCT GGGCAAGTCA TCCAAGTGAA AAACGTCCTG 
AAGTCATTCT TAAGGAATAA ACTTGTTAAA TTATTGTTTC CTCCAGTTGT TGTGACCAAC 
AAAAGAGATT TGAAAAAAAT GTTTCAAAGA TTACAAGATT TATCCTTGGA ATACGGAGAA 
GATGTGAATG AGGAAGATAA TGATGACGAA GCTATACATA CAAAGTCTCG GTCCTATTGT 
CGTAACAAAA AAGCCGAAAA CTCAAAAAAG AAATCCCCTA AATCGAATAA AAAACCCAAG 
AGAAAGAAGC AGAAGTTCTT CACGAGCTGG TTCACTTGGG GCATTTCAAT TACTATAGGA 
ATTAGTTTTG GATGTTGTGT GACATACTTT GTCACTGCAG CATACGAACA CCAAACAGTT 
AAGTCTTTAA GTTTGCGGCC CTCCATATTG GCCTCTTTAC TTTCCTTAGA TTCCTCTAGC 
GACACAATCA ATACGCCCGC CACTGCATCT CCTTCTTCAA CTGAGCAGTT TTTATGGTTT 
GATAAGGGTA CGCTTCAGAT AAACTTCCAC TCCGATGGAT TCATTATGAA AAGTCTTACC 
ATAATCAAGG AGACTTGGGG CAAAATGAAT ACTTTTGTCT TGCATGCTCT TTCTAAACCT 
TTAAAATTTT TGGAGAACCT TAATAAAAGC TCTGAATTTT CCATTGATGA ATCTAATCGG 
ATTTTGGCAC TGGGTTATAT TCTATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ATG32 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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