PTP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PTP2
Entrez Gene ID	854383
Full Name	tyrosine protein phosphatase PTP2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183627.3	NP_014851.3	tyrosine protein phosphatase PTP2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PTP2	NP_014851.3
Kluyveromyces lactis	KLLA0D06611g	XP_453355.1
Eremothecium gossypii	AGOS_ACR158W	NP_983560.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PTP2 and MSG5 are key regulatory PPases that prevent over-activation of the calcium-induced signaling cascade under the parallel control of the SLT2 and calcineurin pathways.
Title: Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration.

Data discovered that six non-essential protein kinase (PKase) disruptions (Deltabck1, Deltamkk1, Deltaslt2/Deltampk1, Deltamck1, Deltassk2 and Deltayak1) suppressed the Cas-phenotype of the Deltaptp2 Deltamsg5 double disruptant.
Title: Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant.

These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway.
Title: Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis.

The Hog 1 common docking & PBD-2 docking sites are also involved in the specific interaction between Hog1 & Ptp2 and govern the dynamic dephosphorylation of activated Hog1.
Title: Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase.

The following PTP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03506	NM_001183627.3 Latest version!	Saccharomyces cerevisiae S288C tyrosine protein phosphatase PTP2 (PTP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTP2 ( NM_001183627.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014851.3 Saccharomyces cerevisiae S288C tyrosine protein phosphatase PTP2 (PTP2), partial mRNA.

Price & Availability↑

CloneID	OSi03506
Clone ID Related Accession (Same CDS sequence)	NM_001183627.3
Accession Version	NM_001183627.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	tyrosine protein phosphatase PTP2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). On Jul 30, 2012 this sequence version replaced NM_001183627.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATCGCA TAGCACAGCA ATATCGTAAT GGCAAAAGAG ACAATAACGG CAATAGAATG 
GCTTCTTCCG CTATATCGGA AAAGGGCCAC ATACAAGTCA ATCAAACTAG AACACCTGGT 
CAAATGCCCG TCTATAGAGG TGAAACTATA AATCTGTCTA ACCTTCCCCA AAATCAAATT 
AAACCGTGCA AAGATTTGGA CGACGTTAAC ATACGGCGGA ACAACTCTAA TAGGCATTCT 
AAAATACTTT TACTAGATCT GTGCGCTGGC CCCAATACCA ACTCATTTTT AGGCAATACC 
AATGCTAAGG ATATCACAGT TTTATCGTTG CCGCTACCCA GCACTTTGGT GAAAAGGTCG 
AACTACCCGT TCGAGAACTT ACTAAAGAAT TACCTTGGAT CTGATGAAAA GTATATTGAG 
TTCACAAAGA TCATCAAAGA TTATGATATT TTCATTTTCA GTGATTCGTT TAGCAGAATT 
TCGAGTTGTT TAAAGACAAC TTTTTGCCTC ATTGAGAAGT TTAAAAAGTT CATCTGCCAT 
TTTTTTCCAT CTCCTTATTT GAAATTCTTT CTTCTCGAAG GCTCTCTGAA TGATAGCAAG 
GCCCCCTCAT TAGGAAAAAA TAAGAAAAAT TGCATCTTGC CCAAATTGGA TTTGAACTTG 
AATGTAAACT TAACTTCAAG GTCAACTTTA AATTTAAGAA TAAACATACC TCCACCCAAT 
GATTCAAATA AAATATTTTT ACAGTCTCTG AAAAAGGATC TAATTCATTA TTCTCCTAAT 
TCTTTGCAAA AGTTTTTCCA ATTCAATATG CCTGCTGACT TAGCACCTAA CGACACGATT 
TTACCGAATT GGCTAAAATT CTGCTCCGTA AAAGAAAATG AAAAGGTAAT ATTAAAGAAA 
CTCTTTAACA ATTTTGAAAC TTTAGAAAAT TTTGAAATGC AAAGATTAGA GAAATGCCTG 
AAATTCAAGA AAAAGCCTTT ACATCAAAAG CAGCTATCAC AAAAGCAGAG GGGTCCGCAA 
TCCACGGATG ATTCAAAATT ATATTCTTTA ACTAGTTTGC AACGACAGTA TAAAAGTTCT 
TTGAAAAGCA ACATACAGAA AAATCAAAAG CTAAAATTAA TTATACCAAA AAACAACACA 
TCTTCTTCGC CATCACCATT ATCTTCCGAT GATACTATAA TGTCACCAAT AAATGATTAC 
GAACTTACTG AAGGAATTCA GTCTTTTACT AAGAATAGAT ATTCTAATAT CTTACCTTAC 
GAACATTCAA GAGTAAAGTT ACCTCACTCC CCGAAACCAC CTGCAGTTTC TGAAGCATCC 
ACAACCGAAA CTAAAACAGA TAAGTCATAT CCGATGTGTC CCGTAGATGC AAAAAACCAC 
TCCTGCAAAC CGAACGACTA TATCAATGCG AACTATTTGA AGCTCACGCA AATTAATCCT 
GATTTCAAGT ATATTGCTAC CCAAGCTCCG CTTCCTTCTA CGATGGATGA TTTTTGGAAG 
GTTATTACTT TAAATAAAGT TAAAGTAATA ATATCATTGA ATTCTGACGA TGAATTGAAT 
TTAAGAAAAT GGGATATTTA CTGGAATAAT CTGTCATATT CCAACCACAC TATCAAACTT 
CAGAACACCT GGGAGAATAT TTGCAATATT AATGGCTGTG TTCTCAGAGT CTTTCAAGTC 
AAGAAAACAG CTCCACAAAA TGATAATATC AGTCAAGATT GTGACCTTCC GCATAATGGT 
GACCTTACTT CCATTACCAT GGCTGTATCC GAGCCGTTTA TTGTTTACCA ATTACAATAC 
AAGAATTGGT TAGATTCATG CGGCGTAGAT ATGAATGACA TCATTAAACT ACACAAAGTC 
AAAAATTCGT TATTGTTTAA CCCGCAAAGT TTTATTACAA GCCTCGAAAA GGATGTTTGC 
AAGCCTGATT TGATAGATGA TAATAATAGT GAGTTACATC TCGATACAGC AAATTCATCG 
CCACTATTAG TCCATTGTTC TGCAGGGTGT GGAAGAACAG GTGTTTTCGT TACCTTGGAT 
TTCCTACTAA GTATTCTTTC ACCTACAACA AATCACTCAA ACAAGATTGA TGTTTGGAAT 
ATGACTCAGG ACCTTATCTT TATCATAGTG AATGAATTAA GAAAGCAAAG GATTTCAATG 
GTACAGAATC TAACTCAATA TATCGCTTGT TATGAGGCAT TATTAAATTA TTTTGCCCTG 
CAAAAGCAGA TAAAGAACGC GTTACCTTGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014851.3
CDS	1..2253
Translation	MDRIAQQYRNGKRDNNGNRMASSAISEKGHIQVNQTRTPGQMPVYRGETINLSNLPQNQIKPCKDLDDVNIRRNNSNRHSKILLLDLCAGPNTNSFLGNTNAKDITVLSLPLPSTLVKRSNYPFENLLKNYLGSDEKYIEFTKIIKDYDIFIFSDSFSRISSCLKTTFCLIEKFKKFICHFFPSPYLKFFLLEGSLNDSKAPSLGKNKKNCILPKLDLNLNVNLTSRSTLNLRINIPPPNDSNKIFLQSLKKDLIHYSPNSLQKFFQFNMPADLAPNDTILPNWLKFCSVKENEKVILKKLFNNFETLENFEMQRLEKCLKFKKKPLHQKQLSQKQRGPQSTDDSKLYSLTSLQRQYKSSLKSNIQKNQKLKLIIPKNNTSSSPSPLSSDDTIMSPINDYELTEGIQSFTKNRYSNILPYEHSRVKLPHSPKPPAVSEASTTETKTDKSYPMCPVDAKNHSCKPNDYINANYLKLTQINPDFKYIATQAPLPSTMDDFWKVITLNKVKVIISLNSDDELNLRKWDIYWNNLSYSNHTIKLQNTWENICNINGCVLRVFQVKKTAPQNDNISQDCDLPHNGDLTSITMAVSEPFIVYQLQYKNWLDSCGVDMNDIIKLHKVKNSLLFNPQSFITSLEKDVCKPDLIDDNNSELHLDTANSSPLLVHCSAGCGRTGVFVTLDFLLSILSPTTNHSNKIDVWNMTQDLIFIIVNELRKQRISMVQNLTQYIACYEALLNYFALQKQIKNALPC

Target ORF information:

RefSeq Version	NM_001183627.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C tyrosine protein phosphatase PTP2 (PTP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183627.3

ORF Insert Sequence:

ATGGATCGCA TAGCACAGCA ATATCGTAAT GGCAAAAGAG ACAATAACGG CAATAGAATG 
GCTTCTTCCG CTATATCGGA AAAGGGCCAC ATACAAGTCA ATCAAACTAG AACACCTGGT 
CAAATGCCCG TCTATAGAGG TGAAACTATA AATCTGTCTA ACCTTCCCCA AAATCAAATT 
AAACCGTGCA AAGATTTGGA CGACGTTAAC ATACGGCGGA ACAACTCTAA TAGGCATTCT 
AAAATACTTT TACTAGATCT GTGCGCTGGC CCCAATACCA ACTCATTTTT AGGCAATACC 
AATGCTAAGG ATATCACAGT TTTATCGTTG CCGCTACCCA GCACTTTGGT GAAAAGGTCG 
AACTACCCGT TCGAGAACTT ACTAAAGAAT TACCTTGGAT CTGATGAAAA GTATATTGAG 
TTCACAAAGA TCATCAAAGA TTATGATATT TTCATTTTCA GTGATTCGTT TAGCAGAATT 
TCGAGTTGTT TAAAGACAAC TTTTTGCCTC ATTGAGAAGT TTAAAAAGTT CATCTGCCAT 
TTTTTTCCAT CTCCTTATTT GAAATTCTTT CTTCTCGAAG GCTCTCTGAA TGATAGCAAG 
GCCCCCTCAT TAGGAAAAAA TAAGAAAAAT TGCATCTTGC CCAAATTGGA TTTGAACTTG 
AATGTAAACT TAACTTCAAG GTCAACTTTA AATTTAAGAA TAAACATACC TCCACCCAAT 
GATTCAAATA AAATATTTTT ACAGTCTCTG AAAAAGGATC TAATTCATTA TTCTCCTAAT 
TCTTTGCAAA AGTTTTTCCA ATTCAATATG CCTGCTGACT TAGCACCTAA CGACACGATT 
TTACCGAATT GGCTAAAATT CTGCTCCGTA AAAGAAAATG AAAAGGTAAT ATTAAAGAAA 
CTCTTTAACA ATTTTGAAAC TTTAGAAAAT TTTGAAATGC AAAGATTAGA GAAATGCCTG 
AAATTCAAGA AAAAGCCTTT ACATCAAAAG CAGCTATCAC AAAAGCAGAG GGGTCCGCAA 
TCCACGGATG ATTCAAAATT ATATTCTTTA ACTAGTTTGC AACGACAGTA TAAAAGTTCT 
TTGAAAAGCA ACATACAGAA AAATCAAAAG CTAAAATTAA TTATACCAAA AAACAACACA 
TCTTCTTCGC CATCACCATT ATCTTCCGAT GATACTATAA TGTCACCAAT AAATGATTAC 
GAACTTACTG AAGGAATTCA GTCTTTTACT AAGAATAGAT ATTCTAATAT CTTACCTTAC 
GAACATTCAA GAGTAAAGTT ACCTCACTCC CCGAAACCAC CTGCAGTTTC TGAAGCATCC 
ACAACCGAAA CTAAAACAGA TAAGTCATAT CCGATGTGTC CCGTAGATGC AAAAAACCAC 
TCCTGCAAAC CGAACGACTA TATCAATGCG AACTATTTGA AGCTCACGCA AATTAATCCT 
GATTTCAAGT ATATTGCTAC CCAAGCTCCG CTTCCTTCTA CGATGGATGA TTTTTGGAAG 
GTTATTACTT TAAATAAAGT TAAAGTAATA ATATCATTGA ATTCTGACGA TGAATTGAAT 
TTAAGAAAAT GGGATATTTA CTGGAATAAT CTGTCATATT CCAACCACAC TATCAAACTT 
CAGAACACCT GGGAGAATAT TTGCAATATT AATGGCTGTG TTCTCAGAGT CTTTCAAGTC 
AAGAAAACAG CTCCACAAAA TGATAATATC AGTCAAGATT GTGACCTTCC GCATAATGGT 
GACCTTACTT CCATTACCAT GGCTGTATCC GAGCCGTTTA TTGTTTACCA ATTACAATAC 
AAGAATTGGT TAGATTCATG CGGCGTAGAT ATGAATGACA TCATTAAACT ACACAAAGTC 
AAAAATTCGT TATTGTTTAA CCCGCAAAGT TTTATTACAA GCCTCGAAAA GGATGTTTGC 
AAGCCTGATT TGATAGATGA TAATAATAGT GAGTTACATC TCGATACAGC AAATTCATCG 
CCACTATTAG TCCATTGTTC TGCAGGGTGT GGAAGAACAG GTGTTTTCGT TACCTTGGAT 
TTCCTACTAA GTATTCTTTC ACCTACAACA AATCACTCAA ACAAGATTGA TGTTTGGAAT 
ATGACTCAGG ACCTTATCTT TATCATAGTG AATGAATTAA GAAAGCAAAG GATTTCAATG 
GTACAGAATC TAACTCAATA TATCGCTTGT TATGAGGCAT TATTAAATTA TTTTGCCCTG 
CAAAAGCAGA TAAAGAACGC GTTACCTTGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PTP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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