SLK19 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SLK19
Entrez Gene ID	854370
Full Name	Slk19p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183614.3	NP_014838.3	Slk19p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR229W	NP_986895.2
Kluyveromyces lactis	KLLA0F00770g	XP_455114.1
Saccharomyces cerevisiae (baker's yeast)	SLK19	NP_014838.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Therefore Slk19 likely acts as kinetochore glue that clusters kinetochores to facilitate efficient and faithful chromosome segregation.
Title: Slk19 clusters kinetochores and facilitates chromosome bipolar attachment.

in early anaphase one region of Slk19p is essential for FEAR signaling, while later in anaphase another region is critical for maintaining the coordination between spindle elongation and the growth of interpolar microtubules.
Title: Slk19p of Saccharomyces cerevisiae regulates anaphase spindle dynamics through two independent mechanisms.

Deletion of SLK19, eliminated the mid-anaphase pause, caused premature anaphase onset and defects in DNA division during anaphase, and reduced viability in kinesin-5-mutated cells
Title: Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells.

Slk19 participates in regulating nuclear migration and, in conjunction with cohesin complex, may be involved in the maintenance of centromeric tensile strength to resist the pole-ward pull.
Title: Deficiency of centromere-associated protein Slk19 causes premature nuclear migration and loss of centromeric elasticity.

The following SLK19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLK19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03496	NM_001183614.3 Latest version!	Saccharomyces cerevisiae S288C Slk19p (SLK19), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLK19 ( NM_001183614.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014838.3 Saccharomyces cerevisiae S288C Slk19p (SLK19), partial mRNA.

Price & Availability↑

CloneID	OSi03496
Clone ID Related Accession (Same CDS sequence)	NM_001183614.3
Accession Version	NM_001183614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Slk19p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). On Jul 30, 2012 this sequence version replaced NM_001183614.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACGAAG TTCCTACCAC TCCAGTTAGG CTTATTTTAG GGCAAGCTCA GCAACGCGAG 
CAGAATTCAG AAAACTGTTC GCAAGAACGT AACCCACGCA CGTTTAATTC CGAACCGGAT 
TCCAGCTTTA ACTCTCCTGG TTCCTCCCAG TTCGTGATAC ATCCTCATGA ACCGCTCGAG 
AAGGAGAAAG ATGAGAAGCA GGATTTAGAC CGCTCGATTG ATTACGGTAG GTCATCAGCT 
CTTAACAACA AGAATAATGC CAATCCATTA GAGAATATAG ACATAAATAA AATGTTTGAT 
GACAAAAAAT CAGATTCAGG TACCAATGAT GATAAAGGAG GCGCAAGTAC TAGTGATAAG 
CATGTCCTTG CATTGAATTA TTCACCGATC AGAGTTGAAA TGAACTCCTC AGAGAAACGA 
AGCGACAAAA ACGTGGACGT AGATGAAAAT GATAAAGAAG GTAGCCATAT TAATAAAAAA 
TTGAAATTGC AGTTAGAATC AGTACCTGAT TTGAAACAAA GCTCTACTAA AGATATCATT 
AATGACAAAG AAGAAATTAT GTCGTCTCCA ATGGCGATTG ATATGATTGA GACCAACATA 
AGCCCAAATA AATTCATAAT AAACGACGGA GTTGAAAGAA ACGATAGTTT CAATATCAAT 
ACCGACACAC TTAAATTAGA AAATGATATA AATGAGAAAC AACAAGAGGA AGACTTCATA 
AAATCTAACA GTAACAATGT GGTTAATATT GACAATGCTT ATAAGGAAAA GGAGGACGAA 
GAAAATGATA TAACAAATTC TCATATAAAC AGGCTTACTC CTTTGTATGA GACTTCTGCA 
AGGGAGTCGA ACTCAAATGA AGAGGGGAGG AATGACTACG ATGATGACAA CCAACTTGAT 
ATTAGACATG ACAATTTTCA GATTGTAGCA AAAAGAAACG AGGAGCTCAC TGATCAGATA 
TATCACTTAA ATCAAATGTT AAACTCTTTG ATTTCCAAAA ATGAATCATT GTCTTTTCAA 
TACGAGAAAT TGAACAAAAA TCACCAACTA CTAATAGATT TGACAAATGA AAAGTTAGAT 
AAACTAAATA CAGAAAGAGA GAGCGATATT GCCAAGGTGG AAAAATTCAA AAAGAGGATT 
AAGGAGTTGA ATACAGAAAT TAAGGTATTG AATTCCAACC AAAAAATATT ACAAGAAAAA 
TTCGATGCCT CAATTACTGA AGTAAATCAT ATAAAGGGCG AACATGAAAA TACTGTCAAC 
ACTTTGCAGC AAAATGAGAA AATTTTAAAT GATAAAAATG TAGAACTGGA AAATATGAAA 
GCAGAATTGA AAGGCAATAA TGATAAATTA TCCGAATATG AGACCACGTT AAATGACTTG 
AATTCTCGCA TTGTGCAACT GAATGATAAG ATTGAAAGCA CAGATATTGT TTTGAAATCT 
AAAGAGAACG AACTAGATAA TTTAAAATTG AGTTTGAAGG AAACTTTATC GATTTCTAAG 
GACTTCAACG ACTCTGACCT TATAGGACAA ATTAATGAGC TAATATCTAC AAAAAACAAC 
TTGCAACAAA AAATGGATGA TTTGAATAAC CTGAACGACG ATAATTTGAA GGTAGTACAG 
GATAAATTAA TTAAAAATGA GGAGACGCTA AAACTAAAAG AAGCTGAAAT TGATTCATTG 
AATAGTGAAA TGGACGAGCT AAAAAAACAG ATCACTTCAA AAGATGATGA ATTTAAGATG 
TGGCAAAGTA AATACGAAAC TGTCGAAGAC GAAGCTAAGA TACGCAATGC CGAAGTTACA 
GAGTTGAACG GAGATATTGA AGATCTAAAG GAATCAAAAT TGCATTTGGA AGAAACAATT 
ACGGAACTAG AAAACAAAGT TCATAAACTA GAAAATGAGT GTGAACTGGA GAAACAAAAA 
TTCGAAAAAA CTTCGCTAGA ATTAGAAAGC TTACAACTAA AAAATAGTAA CATTCAGGCT 
GAGCACATCA AAGAATTGGA AAATCTTCAT GAAAACCTTA TTTCTCTTCA AAATGAATTG 
AAGATTTCAT CCGACAGGAT AACAACATTA ACAAAAGAAA ATGAAGTATT AATGGAACAA 
AATAATAATA ACAATAATAG CGTTACTTTA AGCAATGATC AAAAGGATAG AGATGATGAA 
AAAATTAAAT CATTAGGAAA ACAAGTACAA GATTGGAAAG AAAAGTATGA GGCCAAAGAA 
AAAGATACAA ATAAAAGGCT AAAGTTATTA GCAGAAGATT TGTATATCCA GTATTCATCC 
AAACACGAAC AAAAGGTCAA ACTGTTGAAA AAAGGCTATG AAAACAAGTA CCAAAATAAG 
TTTGATCAAT TAAATTTAGA AAATAAAACT CTGTCAGAAG AAATCGAGCA ATTGAACAAG 
CAGCTGTCTT CAGAAAGAGA AGAAAAGCAG GAGTTACTCA AGTTGTTAGA AAATGAAAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014838.3
CDS	1..2466
Translation	MNEVPTTPVRLILGQAQQREQNSENCSQERNPRTFNSEPDSSFNSPGSSQFVIHPHEPLEKEKDEKQDLDRSIDYGRSSALNNKNNANPLENIDINKMFDDKKSDSGTNDDKGGASTSDKHVLALNYSPIRVEMNSSEKRSDKNVDVDENDKEGSHINKKLKLQLESVPDLKQSSTKDIINDKEEIMSSPMAIDMIETNISPNKFIINDGVERNDSFNINTDTLKLENDINEKQQEEDFIKSNSNNVVNIDNAYKEKEDEENDITNSHINRLTPLYETSARESNSNEEGRNDYDDDNQLDIRHDNFQIVAKRNEELTDQIYHLNQMLNSLISKNESLSFQYEKLNKNHQLLIDLTNEKLDKLNTERESDIAKVEKFKKRIKELNTEIKVLNSNQKILQEKFDASITEVNHIKGEHENTVNTLQQNEKILNDKNVELENMKAELKGNNDKLSEYETTLNDLNSRIVQLNDKIESTDIVLKSKENELDNLKLSLKETLSISKDFNDSDLIGQINELISTKNNLQQKMDDLNNLNDDNLKVVQDKLIKNEETLKLKEAEIDSLNSEMDELKKQITSKDDEFKMWQSKYETVEDEAKIRNAEVTELNGDIEDLKESKLHLEETITELENKVHKLENECELEKQKFEKTSLELESLQLKNSNIQAEHIKELENLHENLISLQNELKISSDRITTLTKENEVLMEQNNNNNNSVTLSNDQKDRDDEKIKSLGKQVQDWKEKYEAKEKDTNKRLKLLAEDLYIQYSSKHEQKVKLLKKGYENKYQNKFDQLNLENKTLSEEIEQLNKQLSSEREEKQELLKLLENEKK

Target ORF information:

RefSeq Version	NM_001183614.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Slk19p (SLK19), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183614.3

ORF Insert Sequence:

ATGAACGAAG TTCCTACCAC TCCAGTTAGG CTTATTTTAG GGCAAGCTCA GCAACGCGAG 
CAGAATTCAG AAAACTGTTC GCAAGAACGT AACCCACGCA CGTTTAATTC CGAACCGGAT 
TCCAGCTTTA ACTCTCCTGG TTCCTCCCAG TTCGTGATAC ATCCTCATGA ACCGCTCGAG 
AAGGAGAAAG ATGAGAAGCA GGATTTAGAC CGCTCGATTG ATTACGGTAG GTCATCAGCT 
CTTAACAACA AGAATAATGC CAATCCATTA GAGAATATAG ACATAAATAA AATGTTTGAT 
GACAAAAAAT CAGATTCAGG TACCAATGAT GATAAAGGAG GCGCAAGTAC TAGTGATAAG 
CATGTCCTTG CATTGAATTA TTCACCGATC AGAGTTGAAA TGAACTCCTC AGAGAAACGA 
AGCGACAAAA ACGTGGACGT AGATGAAAAT GATAAAGAAG GTAGCCATAT TAATAAAAAA 
TTGAAATTGC AGTTAGAATC AGTACCTGAT TTGAAACAAA GCTCTACTAA AGATATCATT 
AATGACAAAG AAGAAATTAT GTCGTCTCCA ATGGCGATTG ATATGATTGA GACCAACATA 
AGCCCAAATA AATTCATAAT AAACGACGGA GTTGAAAGAA ACGATAGTTT CAATATCAAT 
ACCGACACAC TTAAATTAGA AAATGATATA AATGAGAAAC AACAAGAGGA AGACTTCATA 
AAATCTAACA GTAACAATGT GGTTAATATT GACAATGCTT ATAAGGAAAA GGAGGACGAA 
GAAAATGATA TAACAAATTC TCATATAAAC AGGCTTACTC CTTTGTATGA GACTTCTGCA 
AGGGAGTCGA ACTCAAATGA AGAGGGGAGG AATGACTACG ATGATGACAA CCAACTTGAT 
ATTAGACATG ACAATTTTCA GATTGTAGCA AAAAGAAACG AGGAGCTCAC TGATCAGATA 
TATCACTTAA ATCAAATGTT AAACTCTTTG ATTTCCAAAA ATGAATCATT GTCTTTTCAA 
TACGAGAAAT TGAACAAAAA TCACCAACTA CTAATAGATT TGACAAATGA AAAGTTAGAT 
AAACTAAATA CAGAAAGAGA GAGCGATATT GCCAAGGTGG AAAAATTCAA AAAGAGGATT 
AAGGAGTTGA ATACAGAAAT TAAGGTATTG AATTCCAACC AAAAAATATT ACAAGAAAAA 
TTCGATGCCT CAATTACTGA AGTAAATCAT ATAAAGGGCG AACATGAAAA TACTGTCAAC 
ACTTTGCAGC AAAATGAGAA AATTTTAAAT GATAAAAATG TAGAACTGGA AAATATGAAA 
GCAGAATTGA AAGGCAATAA TGATAAATTA TCCGAATATG AGACCACGTT AAATGACTTG 
AATTCTCGCA TTGTGCAACT GAATGATAAG ATTGAAAGCA CAGATATTGT TTTGAAATCT 
AAAGAGAACG AACTAGATAA TTTAAAATTG AGTTTGAAGG AAACTTTATC GATTTCTAAG 
GACTTCAACG ACTCTGACCT TATAGGACAA ATTAATGAGC TAATATCTAC AAAAAACAAC 
TTGCAACAAA AAATGGATGA TTTGAATAAC CTGAACGACG ATAATTTGAA GGTAGTACAG 
GATAAATTAA TTAAAAATGA GGAGACGCTA AAACTAAAAG AAGCTGAAAT TGATTCATTG 
AATAGTGAAA TGGACGAGCT AAAAAAACAG ATCACTTCAA AAGATGATGA ATTTAAGATG 
TGGCAAAGTA AATACGAAAC TGTCGAAGAC GAAGCTAAGA TACGCAATGC CGAAGTTACA 
GAGTTGAACG GAGATATTGA AGATCTAAAG GAATCAAAAT TGCATTTGGA AGAAACAATT 
ACGGAACTAG AAAACAAAGT TCATAAACTA GAAAATGAGT GTGAACTGGA GAAACAAAAA 
TTCGAAAAAA CTTCGCTAGA ATTAGAAAGC TTACAACTAA AAAATAGTAA CATTCAGGCT 
GAGCACATCA AAGAATTGGA AAATCTTCAT GAAAACCTTA TTTCTCTTCA AAATGAATTG 
AAGATTTCAT CCGACAGGAT AACAACATTA ACAAAAGAAA ATGAAGTATT AATGGAACAA 
AATAATAATA ACAATAATAG CGTTACTTTA AGCAATGATC AAAAGGATAG AGATGATGAA 
AAAATTAAAT CATTAGGAAA ACAAGTACAA GATTGGAAAG AAAAGTATGA GGCCAAAGAA 
AAAGATACAA ATAAAAGGCT AAAGTTATTA GCAGAAGATT TGTATATCCA GTATTCATCC 
AAACACGAAC AAAAGGTCAA ACTGTTGAAA AAAGGCTATG AAAACAAGTA CCAAAATAAG 
TTTGATCAAT TAAATTTAGA AAATAAAACT CTGTCAGAAG AAATCGAGCA ATTGAACAAG 
CAGCTGTCTT CAGAAAGAGA AGAAAAGCAG GAGTTACTCA AGTTGTTAGA AAATGAAAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SLK19 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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