ELG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ELG1
Entrez Gene ID	854315
Full Name	Elg1p
Synonyms	RTT110
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183563.3	NP_014787.3	Elg1p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ELG1	NP_014787.3
Kluyveromyces lactis	KLLA0E03301g	XP_454094.1
Eremothecium gossypii	AGOS_AFR389C	NP_985936.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the various roles of Elg1 in genome stability maintenance involve the unloading of both modified and unmodified PCNA.
Title: A structure-function analysis of the yeast Elg1 protein reveals the importance of PCNA unloading in genome stability maintenance.

results suggest that Elg1-RLC acts as a general PCNA unloader and is dependent upon DNA ligation during chromosome replication.
Title: Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment Ligation.

ELG1 deletion from pds5-1 mutant cells results in a significant rescue of chromatid cohesion, but not condensation, defects
Title: Pds5 regulators segregate cohesion and condensation pathways in Saccharomyces cerevisiae.

Elg1 physically contacts the Mhf1/Mhf2 histone-like complex and genetically interacts with MPH1 (ortholog of the FANCM helicase) and CHL1 (ortholog of the FANCJ helicase) genes.
Title: Genetic and physical interactions between the yeast ELG1 gene and orthologs of the Fanconi anemia pathway.

A high copy number suppressor screen was performed to search for genes that, when overexpressed, suppress the synthetic lethality between elg1Delta and srs2Delta mutants.
Title: A genetic screen for high copy number suppressors of the synthetic lethality between elg1? and srs2? in yeast.

The following ELG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ELG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03446	NM_001183563.3 Latest version!	Saccharomyces cerevisiae S288C Elg1p (ELG1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ELG1 ( NM_001183563.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014787.3 Saccharomyces cerevisiae S288C Elg1p (ELG1), partial mRNA.

Price & Availability↑

CloneID	OSi03446
Clone ID Related Accession (Same CDS sequence)	NM_001183563.3
Accession Version	NM_001183563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Elg1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). On Jul 30, 2012 this sequence version replaced NM_001183563.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAAGGC ACGTGTCTTT ATCTGATATA TTGACAGGAA ATAAGAGAAA AGTGAGAAGA 
CAAGATGCTC TACAGATTAC CATCGATGAC GAAAATGATA CTGAATCAGG CACTTTTGAC 
GCCAGAACAG CCAAACATGA CGATTCTTCA GTAATATTTT TAAACCACTC TGTTGTGAAG 
CCAATTGAAG CTGTTTCTAC TAATCACAAA TCTGCAAAGG AGTTTTTGAT GACGAAACGC 
ACAAAAGAAA AATGCGATGA CGACGATGAT GATCTTATTG TAATCAGTGA TAAGAGTCCC 
AAAAGTGAGA CTAATTGTAG CAAAATTGCA CTTTCGCAGG AGCATGAAGA TGATATTTCT 
ATAATTTCCA CATCGAGAAT CAAATCATCG CTTCTTAATG AAAGAGCATC AAAGATTAAA 
AACTTCCTTA AACATGAGAC AACCGACACG TTCAAAAGAT TAAATAGCAT CTCTAAACTA 
AATGAAATAG AGCCACCATT ACCATTACAT CAATCGATTT TCCCAGTAGG TGACAAAGAG 
CTGTCAGATC GGAGTGTTGA TATCCCGCTT CCTTTCCGCA CAATACCGCC ATTAAATCAT 
AATTTTCTTC CTAGTGACTA TGAATCGTTG AAAGACAAGA ATTCAGCATC TTGTATTCCT 
GTTCGCTATC AAGCACCGGT ATTACTTGGC ACAAATATTA AAAGAAATAC AACTCTCACA 
TGGCCGCAGC TATTCAAGCC TGTCACTTTA AAACAAGTAT TAATTGAACC CAAATTGAAA 
CTTCGAATCA AAAATTGGAT TGAAACATCC TTTCATACGC TAGAAAAGCC TACCTTGAGG 
AACCGTCTAT TGAACAGAAT AAATCCCAAT AAACAACAGG GATCTGGTGA TGAGCTTGCC 
AACTTCATTG TACCTGATCT CGAAGAAGAC GAAAACTTGC GTCCAGATTT CTACAGAAAT 
GGCGAAGCAA ATAGCAGCTT GAGCGAATTT GTTCCACTAA TGATATTACA CGGAAATTCA 
ATAGGCAAGA AAACGCTGAT ACAGACAATC ATGAGAGAGA TTGCTGGCGA CGATAATTCT 
TACCAGATAT ATGAAGTTAA TTCAAACATG AACAGGAGCA AAAAAGATTT GCTTGATATC 
CTACTAGATT TTACCACCAC ACACTATGTC AAAGATTCCT CAAAAAGGAA AAGTGACTAC 
GGGCTTGTTT TATTTAATGA TGTTGATGTT TTATTTAAAG AACATGATAG GGGATATTGG 
GCAATGATCA GCAAGCTTTG TGAATTTTCA CGAAGGCCCC TCGTATTGAC TTGTAAGGAT 
TTGTCACTAG TTCCTAGTGA ACTTATTGCC TTGGCAAGTG AACAAAACTC TTTGTTTCAT 
ACTAAGAAAA TTAGTACATC CACAGTCTAT GCGTTTCTAA CAAAATATTT AAAGTCCCTT 
GAAATCGAAG TTTGTGACGA CTGGCTTCGC GATGTTGTAA AACAAAATAA TGCGGATATT 
AGGAAGTGCT TGATGCACTT GCAGTTTTGG TGCGTTGATA CTGAGGCTGA TTTGATTTCA 
TCCAAGAACA GACTACCGGT CCTAACATCA ACTCTTGGTT CAAGCGTTAA AGATATATCG 
CAATTAACTG ATCTACTCTC CATAAATGAT GTTATTGGTC AAGCAACGCT CAATCGTTCC 
ATGGTTAGAC AAGAAATAGA TTCTACCACA ATGACCCCAG AAAAAGTTAA TACTTTCCAG 
GATCAAAATC TGGATGATGA AATGAAACTA AAATTTGATT ACGTGATAGA TTATAAATTG 
CATTTAAATG ACCCCAATAG ACAACCGTTA TTACCGTTTG AATTGAATAT TTACCAACAC 
ATCCAAGAAC AATTGGAAGC TCGGTATTCC TATGTTAGAG AAGCAAACCA TAGGCTTGAT 
AACGAATACC TTGTTAACAG GTTCAAAAAA ATGACAGAAT CTACATTAAA TTTTTTGGCG 
TCAAGAATAC CGAAGTATGA CCATCTTCAG TCGGCAAGAA GGACGAGAAA TTCGAAGAAA 
ATTTCTGATA TTTTGAACCA ATTCAAAGGA ATTTATAATG ATGAAACTCT GAACGAAAAT 
GCCGAGATTG ATCTGCTGAG CGCCACAACT CAACAAATTA AAGCGGAGAT CAATCCCTTC 
GTTTTTGAAA TCGCTAAAAG TGACGCCAAT GTCAAGAATG AAAACAAACA AATATTTGAG 
TTGCATAGTG AAAATGTATC AGAAAGACGA TACAAAGATT TGGTCTATCA GTTGTCACAA 
GAGGGTGTCT TGAAAAACGT ATGGTTCAAC GCTGATCCGA GCATAGTCGT GAGAAAATGG 
GAGCATTTGC ACTCAGGCTT TTCAAAGAAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014787.3
CDS	1..2376
Translation	MKRHVSLSDILTGNKRKVRRQDALQITIDDENDTESGTFDARTAKHDDSSVIFLNHSVVKPIEAVSTNHKSAKEFLMTKRTKEKCDDDDDDLIVISDKSPKSETNCSKIALSQEHEDDISIISTSRIKSSLLNERASKIKNFLKHETTDTFKRLNSISKLNEIEPPLPLHQSIFPVGDKELSDRSVDIPLPFRTIPPLNHNFLPSDYESLKDKNSASCIPVRYQAPVLLGTNIKRNTTLTWPQLFKPVTLKQVLIEPKLKLRIKNWIETSFHTLEKPTLRNRLLNRINPNKQQGSGDELANFIVPDLEEDENLRPDFYRNGEANSSLSEFVPLMILHGNSIGKKTLIQTIMREIAGDDNSYQIYEVNSNMNRSKKDLLDILLDFTTTHYVKDSSKRKSDYGLVLFNDVDVLFKEHDRGYWAMISKLCEFSRRPLVLTCKDLSLVPSELIALASEQNSLFHTKKISTSTVYAFLTKYLKSLEIEVCDDWLRDVVKQNNADIRKCLMHLQFWCVDTEADLISSKNRLPVLTSTLGSSVKDISQLTDLLSINDVIGQATLNRSMVRQEIDSTTMTPEKVNTFQDQNLDDEMKLKFDYVIDYKLHLNDPNRQPLLPFELNIYQHIQEQLEARYSYVREANHRLDNEYLVNRFKKMTESTLNFLASRIPKYDHLQSARRTRNSKKISDILNQFKGIYNDETLNENAEIDLLSATTQQIKAEINPFVFEIAKSDANVKNENKQIFELHSENVSERRYKDLVYQLSQEGVLKNVWFNADPSIVVRKWEHLHSGFSKNK

Target ORF information:

RefSeq Version	NM_001183563.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Elg1p (ELG1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183563.3

ORF Insert Sequence:

ATGAAAAGGC ACGTGTCTTT ATCTGATATA TTGACAGGAA ATAAGAGAAA AGTGAGAAGA 
CAAGATGCTC TACAGATTAC CATCGATGAC GAAAATGATA CTGAATCAGG CACTTTTGAC 
GCCAGAACAG CCAAACATGA CGATTCTTCA GTAATATTTT TAAACCACTC TGTTGTGAAG 
CCAATTGAAG CTGTTTCTAC TAATCACAAA TCTGCAAAGG AGTTTTTGAT GACGAAACGC 
ACAAAAGAAA AATGCGATGA CGACGATGAT GATCTTATTG TAATCAGTGA TAAGAGTCCC 
AAAAGTGAGA CTAATTGTAG CAAAATTGCA CTTTCGCAGG AGCATGAAGA TGATATTTCT 
ATAATTTCCA CATCGAGAAT CAAATCATCG CTTCTTAATG AAAGAGCATC AAAGATTAAA 
AACTTCCTTA AACATGAGAC AACCGACACG TTCAAAAGAT TAAATAGCAT CTCTAAACTA 
AATGAAATAG AGCCACCATT ACCATTACAT CAATCGATTT TCCCAGTAGG TGACAAAGAG 
CTGTCAGATC GGAGTGTTGA TATCCCGCTT CCTTTCCGCA CAATACCGCC ATTAAATCAT 
AATTTTCTTC CTAGTGACTA TGAATCGTTG AAAGACAAGA ATTCAGCATC TTGTATTCCT 
GTTCGCTATC AAGCACCGGT ATTACTTGGC ACAAATATTA AAAGAAATAC AACTCTCACA 
TGGCCGCAGC TATTCAAGCC TGTCACTTTA AAACAAGTAT TAATTGAACC CAAATTGAAA 
CTTCGAATCA AAAATTGGAT TGAAACATCC TTTCATACGC TAGAAAAGCC TACCTTGAGG 
AACCGTCTAT TGAACAGAAT AAATCCCAAT AAACAACAGG GATCTGGTGA TGAGCTTGCC 
AACTTCATTG TACCTGATCT CGAAGAAGAC GAAAACTTGC GTCCAGATTT CTACAGAAAT 
GGCGAAGCAA ATAGCAGCTT GAGCGAATTT GTTCCACTAA TGATATTACA CGGAAATTCA 
ATAGGCAAGA AAACGCTGAT ACAGACAATC ATGAGAGAGA TTGCTGGCGA CGATAATTCT 
TACCAGATAT ATGAAGTTAA TTCAAACATG AACAGGAGCA AAAAAGATTT GCTTGATATC 
CTACTAGATT TTACCACCAC ACACTATGTC AAAGATTCCT CAAAAAGGAA AAGTGACTAC 
GGGCTTGTTT TATTTAATGA TGTTGATGTT TTATTTAAAG AACATGATAG GGGATATTGG 
GCAATGATCA GCAAGCTTTG TGAATTTTCA CGAAGGCCCC TCGTATTGAC TTGTAAGGAT 
TTGTCACTAG TTCCTAGTGA ACTTATTGCC TTGGCAAGTG AACAAAACTC TTTGTTTCAT 
ACTAAGAAAA TTAGTACATC CACAGTCTAT GCGTTTCTAA CAAAATATTT AAAGTCCCTT 
GAAATCGAAG TTTGTGACGA CTGGCTTCGC GATGTTGTAA AACAAAATAA TGCGGATATT 
AGGAAGTGCT TGATGCACTT GCAGTTTTGG TGCGTTGATA CTGAGGCTGA TTTGATTTCA 
TCCAAGAACA GACTACCGGT CCTAACATCA ACTCTTGGTT CAAGCGTTAA AGATATATCG 
CAATTAACTG ATCTACTCTC CATAAATGAT GTTATTGGTC AAGCAACGCT CAATCGTTCC 
ATGGTTAGAC AAGAAATAGA TTCTACCACA ATGACCCCAG AAAAAGTTAA TACTTTCCAG 
GATCAAAATC TGGATGATGA AATGAAACTA AAATTTGATT ACGTGATAGA TTATAAATTG 
CATTTAAATG ACCCCAATAG ACAACCGTTA TTACCGTTTG AATTGAATAT TTACCAACAC 
ATCCAAGAAC AATTGGAAGC TCGGTATTCC TATGTTAGAG AAGCAAACCA TAGGCTTGAT 
AACGAATACC TTGTTAACAG GTTCAAAAAA ATGACAGAAT CTACATTAAA TTTTTTGGCG 
TCAAGAATAC CGAAGTATGA CCATCTTCAG TCGGCAAGAA GGACGAGAAA TTCGAAGAAA 
ATTTCTGATA TTTTGAACCA ATTCAAAGGA ATTTATAATG ATGAAACTCT GAACGAAAAT 
GCCGAGATTG ATCTGCTGAG CGCCACAACT CAACAAATTA AAGCGGAGAT CAATCCCTTC 
GTTTTTGAAA TCGCTAAAAG TGACGCCAAT GTCAAGAATG AAAACAAACA AATATTTGAG 
TTGCATAGTG AAAATGTATC AGAAAGACGA TACAAAGATT TGGTCTATCA GTTGTCACAA 
GAGGGTGTCT TGAAAAACGT ATGGTTCAAC GCTGATCCGA GCATAGTCGT GAGAAAATGG 
GAGCATTTGC ACTCAGGCTT TTCAAAGAAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ELG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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