ORF » Species Summary » Saccharomyces cerevisiae S288C » AFI1 cDNA ORF clone

AFI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following AFI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03431 NM_001183548.1
Latest version!		 Saccharomyces cerevisiae S288C Afi1p (AFI1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    AFI1 ( NM_001183548.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014772.1 Saccharomyces cerevisiae S288C Afi1p (AFI1), partial mRNA.
    Price & Availability↑
    CloneID OSi03431
    Clone ID Related Accession (Same CDS sequence) NM_001183548.1
    Accession Version NM_001183548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Afi1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGCGAA GAGAACTAAA TAACTCTATC AGCAACAGGT CGATAGAAAA TGAATCCTTT 
    CCTTTTGAAA GGCCGAATGT CAGCTATATA ATATCCGCAG AATTTGATAA TAAATTAGGT 
    CCTATTTTGA AGCACCAATA CCCGAAAGAT ATTCCAGGTT TCAATCAGTT CTCACATGAG 
    CAACGCAACG GCAATACGTC GGTGTCTATG AACTTGGCAA GTTTAATGAT ACCGAGTAGC 
    ATAGAGAGAA ATCCCGGCAA ACAAGATATT ACAGTGTTTA CCCTTTATTA CAATAAGTTT 
    ACTCAAAACT ATCAACTCTT TCCTGTGCCA AAAGACCCTC GTTTCAGTTT CAATCTGCAT 
    CATCGTGAAC AGTCAGATGG TAGTGTTACC AATAGCATAT ATTACGATGC CGAAAATCAT 
    CAAGATGCAA AAAATAATAG ATACACTATT GTCTTGGAAG ATGATGAGCT CGAATGCCAG 
    GAAGTTCAGA ATAATCAGAA AGCCATCGAC AACGAACCTT TATTTTTCAT AAATGTCGCC 
    AACACAGTAT TAGACACCAC GAACGATAGA GGGGCAGTAA TCAAGTCGAT TGCCATCGGA 
    ACACCATTAA AAACCTTCTT TGCATTCAAA AATATTATTG TATTAGTGTT AGACTTGTAT 
    ATGAAAGCTC CCACTCAAGC TGCTGCTACA GACATACTGC TTGATTGCTT TAATATGCTA 
    AACAGTATTG ACCTTACATT GATTAATGAC ATACATTCCA AAAGTAGTAT ACAAGAAGTG 
    CTACACTCTA TCCATGACGA ATCTATCATT ACCAAAGTAT TTTTAGACCC GGATTCCACT 
    TTAAAGAAAC TCTTTTGCAT CAATGGATTT GACACTAAGG ACAAGTATGG TAACATTGTG 
    ACTTTTCATG ACCAACTCAT TCAATATCAT TTTACAAGAT TTCAGCCAAA GACGCTCCCG 
    CCATTTTTGT TAAAAATCCC ATTGCAATTT AATATGATTA GAAGAGAGCC TATTTATATT 
    GAAAATGATT ATAATGAACT AGTTTTAAAA TTTTTGGATA AATTTGTCCC TTACCTTCTT 
    AAAGCCGGAC AAAAAGTTAA TGCATGGAAG TTAGTCATCA ATTCAACTAA ACTGAGTAAA 
    GAAGACCTAT GCGCTTTTAT TTTGTCATTA GCCAACATAA CGGCAACTTA TGCTAGCGAT 
    CCACAATCAT ACTTCAAAGG AAATGCTGCG TTGATTTTTC CCTATATGGA CATTTCTCTT 
    GTTGACGGTT TGCGGGCGTA TGTAGCATCG AATAGTGATT TTGTCGGATG CTTTGCAATT 
    ATCGGAACTG CCAATCCAAT TTTCCGATAC CAGTTAGATA TCTGGGATTA TTACTACGAT 
    GTGGATGAGG GAGTGTTTTA TGAAAATAAT AGTCCAGAAA AAGAAAAGCC AGATACAGTG 
    GCAGAAGTGA AGATTGGGCC TAACCCTTTG AGAAAGATAT TCAATAGGCC TCATTTCTCA 
    ACCAACGCAG TCAACGAAAG TCAGGTAAAC TTGGGCCAGA AGCTCTTTTC TCTTCTTATC 
    GACGAATATC ATGATAGTGA TACTATCATG AGCGTTTTGA GAAGGCTGAA TGTTCTTCAG 
    TTGGAAAACC TTCTGGACGC TTTGAAAAGA CGAGAAATTC CACCTAATAT AGCACTAAAA 
    GATGAATATA TCATGTTTTA TAAAGATTTT TTCATTTTCC CGGAGTTCTT TGATTATTTT 
    ACACTGCATA GTATTGAACT ATTGTCTAAC TTGGATAATT GTTTATTTAG CTTAGGAAAC 
    ACATGCCAGT TATTTTCCAC AGAGCAAATA TATTCCCAAT TAAGCCAAAT ATTGGACATT 
    GTTAAAGAGC TGTTTCGCAT GGTATCGGTA AGCAGGACAA ACATTGAGAA GTTTTTAAAC 
    GCTTGTTTGA ACTATTCTCC GTTCAAAATA TTACCCACAG CTCAGTTACA CGGTGATAAT 
    ATTTCTAGGT GGAGTTTTGA AAGCGAAGTG CGTCAGGGTT TCGATAATTT TAACAGCTAT 
    ATGGGCATCG AAAAAGATCC CCACGGTGTT ATAGTATCAG CAATAGATCT TTTTACTCAA 
    ATTTACAGTT TTGATATCTT GGCGTTTTTC CTCACATTTA TTACAAAAGA AAGTGGGCAG 
    GATTTGCCCT TCACAAAATC TCTGTCCAGG AGAAGAACGT ATTTGACCAG AATTGCTCAA 
    TCTTCTTCAT TGAGACAGTT TTTACAACTG AGTACACGCC CTAATATAAG GATTCTGGGA 
    GGTAATGGGC AGGGTACTGG AAATTCCAAT TATCCCGAAT TTACAAACGC ATCCTCAGTC 
    ATCTCACCCA AGCTAAGGGC TTCTCCTCTA CTAGAAAGAA GGGCGTCAAA AATCTGCTAT 
    GCTATTACGA AATTACTCTA TAGGTTAGAA TGTCATCCCA TAGGTATGGC CCTTTTAAAG 
    AAGTATCTCC ACAATCAATT GCGAGAAGCC TATCTAGAAT CAAAACGACA CTTTATTTCC 
    AAAAAGGGAG ATTCGACCAA CACTTCCTCT ACCATTGCAT CATCATCTTT CGCTGGCGCA 
    TCTGTTCCTC TATCTTCAAA CGAATCAGGA ATGCTAAACG GCTTGAAGCA AATTAACGAA 
    CAACAAGAAT CTACATTAGA AACCACTCAA AAGGAAGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014772.1
    CDS1..2682
    Translation

    Target ORF information:

    RefSeq Version NM_001183548.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Afi1p (AFI1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001183548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGCGAA GAGAACTAAA TAACTCTATC AGCAACAGGT CGATAGAAAA TGAATCCTTT 
    CCTTTTGAAA GGCCGAATGT CAGCTATATA ATATCCGCAG AATTTGATAA TAAATTAGGT 
    CCTATTTTGA AGCACCAATA CCCGAAAGAT ATTCCAGGTT TCAATCAGTT CTCACATGAG 
    CAACGCAACG GCAATACGTC GGTGTCTATG AACTTGGCAA GTTTAATGAT ACCGAGTAGC 
    ATAGAGAGAA ATCCCGGCAA ACAAGATATT ACAGTGTTTA CCCTTTATTA CAATAAGTTT 
    ACTCAAAACT ATCAACTCTT TCCTGTGCCA AAAGACCCTC GTTTCAGTTT CAATCTGCAT 
    CATCGTGAAC AGTCAGATGG TAGTGTTACC AATAGCATAT ATTACGATGC CGAAAATCAT 
    CAAGATGCAA AAAATAATAG ATACACTATT GTCTTGGAAG ATGATGAGCT CGAATGCCAG 
    GAAGTTCAGA ATAATCAGAA AGCCATCGAC AACGAACCTT TATTTTTCAT AAATGTCGCC 
    AACACAGTAT TAGACACCAC GAACGATAGA GGGGCAGTAA TCAAGTCGAT TGCCATCGGA 
    ACACCATTAA AAACCTTCTT TGCATTCAAA AATATTATTG TATTAGTGTT AGACTTGTAT 
    ATGAAAGCTC CCACTCAAGC TGCTGCTACA GACATACTGC TTGATTGCTT TAATATGCTA 
    AACAGTATTG ACCTTACATT GATTAATGAC ATACATTCCA AAAGTAGTAT ACAAGAAGTG 
    CTACACTCTA TCCATGACGA ATCTATCATT ACCAAAGTAT TTTTAGACCC GGATTCCACT 
    TTAAAGAAAC TCTTTTGCAT CAATGGATTT GACACTAAGG ACAAGTATGG TAACATTGTG 
    ACTTTTCATG ACCAACTCAT TCAATATCAT TTTACAAGAT TTCAGCCAAA GACGCTCCCG 
    CCATTTTTGT TAAAAATCCC ATTGCAATTT AATATGATTA GAAGAGAGCC TATTTATATT 
    GAAAATGATT ATAATGAACT AGTTTTAAAA TTTTTGGATA AATTTGTCCC TTACCTTCTT 
    AAAGCCGGAC AAAAAGTTAA TGCATGGAAG TTAGTCATCA ATTCAACTAA ACTGAGTAAA 
    GAAGACCTAT GCGCTTTTAT TTTGTCATTA GCCAACATAA CGGCAACTTA TGCTAGCGAT 
    CCACAATCAT ACTTCAAAGG AAATGCTGCG TTGATTTTTC CCTATATGGA CATTTCTCTT 
    GTTGACGGTT TGCGGGCGTA TGTAGCATCG AATAGTGATT TTGTCGGATG CTTTGCAATT 
    ATCGGAACTG CCAATCCAAT TTTCCGATAC CAGTTAGATA TCTGGGATTA TTACTACGAT 
    GTGGATGAGG GAGTGTTTTA TGAAAATAAT AGTCCAGAAA AAGAAAAGCC AGATACAGTG 
    GCAGAAGTGA AGATTGGGCC TAACCCTTTG AGAAAGATAT TCAATAGGCC TCATTTCTCA 
    ACCAACGCAG TCAACGAAAG TCAGGTAAAC TTGGGCCAGA AGCTCTTTTC TCTTCTTATC 
    GACGAATATC ATGATAGTGA TACTATCATG AGCGTTTTGA GAAGGCTGAA TGTTCTTCAG 
    TTGGAAAACC TTCTGGACGC TTTGAAAAGA CGAGAAATTC CACCTAATAT AGCACTAAAA 
    GATGAATATA TCATGTTTTA TAAAGATTTT TTCATTTTCC CGGAGTTCTT TGATTATTTT 
    ACACTGCATA GTATTGAACT ATTGTCTAAC TTGGATAATT GTTTATTTAG CTTAGGAAAC 
    ACATGCCAGT TATTTTCCAC AGAGCAAATA TATTCCCAAT TAAGCCAAAT ATTGGACATT 
    GTTAAAGAGC TGTTTCGCAT GGTATCGGTA AGCAGGACAA ACATTGAGAA GTTTTTAAAC 
    GCTTGTTTGA ACTATTCTCC GTTCAAAATA TTACCCACAG CTCAGTTACA CGGTGATAAT 
    ATTTCTAGGT GGAGTTTTGA AAGCGAAGTG CGTCAGGGTT TCGATAATTT TAACAGCTAT 
    ATGGGCATCG AAAAAGATCC CCACGGTGTT ATAGTATCAG CAATAGATCT TTTTACTCAA 
    ATTTACAGTT TTGATATCTT GGCGTTTTTC CTCACATTTA TTACAAAAGA AAGTGGGCAG 
    GATTTGCCCT TCACAAAATC TCTGTCCAGG AGAAGAACGT ATTTGACCAG AATTGCTCAA 
    TCTTCTTCAT TGAGACAGTT TTTACAACTG AGTACACGCC CTAATATAAG GATTCTGGGA 
    GGTAATGGGC AGGGTACTGG AAATTCCAAT TATCCCGAAT TTACAAACGC ATCCTCAGTC 
    ATCTCACCCA AGCTAAGGGC TTCTCCTCTA CTAGAAAGAA GGGCGTCAAA AATCTGCTAT 
    GCTATTACGA AATTACTCTA TAGGTTAGAA TGTCATCCCA TAGGTATGGC CCTTTTAAAG 
    AAGTATCTCC ACAATCAATT GCGAGAAGCC TATCTAGAAT CAAAACGACA CTTTATTTCC 
    AAAAAGGGAG ATTCGACCAA CACTTCCTCT ACCATTGCAT CATCATCTTT CGCTGGCGCA 
    TCTGTTCCTC TATCTTCAAA CGAATCAGGA ATGCTAAACG GCTTGAAGCA AATTAACGAA 
    CAACAAGAAT CTACATTAGA AACCACTCAA AAGGAAGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
    Nature387(6632 Suppl)98-102(1997 May)
    Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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