Gene Symbol | OST3 |
Entrez Gene ID | 854252 |
Full Name | dolichyl-diphosphooligosaccharide--protein glycotransferase OST3 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » OST3 cDNA ORF clone
Gene Symbol | OST3 |
Entrez Gene ID | 854252 |
Full Name | dolichyl-diphosphooligosaccharide--protein glycotransferase OST3 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001183504.1 | NP_014728.1 | dolichyl-diphosphooligosaccharide--protein glycotransferase OST3 |
Gallus gallus (chicken) | TUSC3 | XP_420692.2 |
Drosophila melanogaster (fruit fly) | Ostgamma | NP_609204.2 |
Canis lupus familiaris (dog) | TUSC3 | XP_532823.4 |
Bos taurus (cattle) | TUSC3 | NP_001074383.1 |
Mus musculus (house mouse) | Tusc3 | NP_084530.3 |
Danio rerio (zebrafish) | tusc3 | NP_001018396.1 |
Saccharomyces cerevisiae (baker's yeast) | OST3 | NP_014728.1 |
Eremothecium gossypii | AGOS_AER413C | NP_985268.2 |
Pan troglodytes (chimpanzee) | TUSC3 | XP_001140529.1 |
Rattus norvegicus (Norway rat) | Tusc3 | NP_001004212.1 |
TUSC3 | XP_001093732.2 | |
Kluyveromyces lactis | KLLA0C13904g | XP_452821.1 |
Schizosaccharomyces pombe (fission yeast) | SPAPB17E12.11 | NP_001018226.1 |
Magnaporthe oryzae (rice blast fungus) | MGG_04500 | XP_003710955.1 |
Neurospora crassa | NCU03995 | XP_956580.1 |
Xenopus tropicalis (tropical clawed frog) | tusc3 | XP_002934712.1 |
Homo sapiens (human) | TUSC3 | NP_839952.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP010010 | XP_319154.4 |
Caenorhabditis elegans (roundworm) | ZK686.3 | NP_498691.1 |
The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
OST3 and OST6 oligosaccharyltransferase subunits bind polypeptide substrate to locally enhance N-glycosylation.
Title: Oligosaccharyltransferase subunits bind polypeptide substrate to locally enhance N-glycosylation.
these assays showed that the thioredoxin-like active sites of Ost3p and Ost6p could form transient mixed disulfide bonds with cysteines in a model substrate glycoprotein, consistent with the function of Ost3p and Ost6p in modulating N-glycosylation substrate selection by OTase in vivo.
Title: Mixed disulfide formation in vitro between a glycoprotein substrate and yeast oligosaccharyltransferase subunits Ost3p and Ost6p.
Data support a model of Ost3/6p function in which they transiently bind stretches of nascent polypeptide substrate to inhibit protein folding, thereby increasing glycosylation efficiency at nearby asparagine residues.
Title: Polypeptide binding specificities of Saccharomyces cerevisiae oligosaccharyltransferase accessory proteins Ost3p and Ost6p.
Mutation of the active-site cysteine residues of Ost6p and its paralogue Ost3p affected the glycosylation efficiency of a subset of glycosylation sites.
Title: Oxidoreductase activity of oligosaccharyltransferase subunits Ost3p and Ost6p defines site-specific glycosylation efficiency.
The paralogues Ost3p and Ost6p were required for efficient glycosylation of distinct defined glycosylation sites.
Title: Analysis of glycosylation site occupancy reveals a role for Ost3p and Ost6p in site-specific N-glycosylation efficiency.
The following OST3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OST3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi03391 | |
Clone ID Related Accession (Same CDS sequence) | NM_001183504.1 | |
Accession Version | NM_001183504.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | dolichyl-diphosphooligosaccharide--protein glycotransferase OST3 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGAATTGGC TGTTTTTGGT CTCGCTGGTT TTCTTCTGCG GCGTGTCAAC CCATCCTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_014728.1 |
CDS | 1..1053 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001183504.1 |
1 | ATGAATTGGC TGTTTTTGGT CTCGCTGGTT TTCTTCTGCG GCGTGTCAAC CCATCCTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XV. |
Life with 6000 genes. |