DNL4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DNL4
Entrez Gene ID	854166
Full Name	DNA ligase (ATP) DNL4
Synonyms	LIG4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183424.1	NP_014647.1	DNA ligase (ATP) DNL4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lig4	NP_001099565.1
Drosophila melanogaster (fruit fly)	Lig4	NP_572907.1
Magnaporthe oryzae (rice blast fungus)	MGG_12899	XP_003712808.1
Bos taurus (cattle)	LIG4	NP_001178055.1
Saccharomyces cerevisiae (baker's yeast)	DNL4	NP_014647.1
Eremothecium gossypii	AGOS_ACR008W	NP_983411.1
Gallus gallus (chicken)	LIG4	NP_001025987.1
Kluyveromyces lactis	KLLA0D01089g	XP_453119.1
Neurospora crassa	NCU06264	XP_962868.1
Mus musculus (house mouse)	Lig4	NP_795927.2
Danio rerio (zebrafish)	lig4	NP_001096593.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000623	XP_310455.4
Arabidopsis thaliana (thale cress)	ATLIG4	NP_568851.2
Homo sapiens (human)	LIG4	NP_001091738.1
Pan troglodytes (chimpanzee)	LIG4	XP_003314254.1
Canis lupus familiaris (dog)	LIG4	XP_005634152.1
Schizosaccharomyces pombe (fission yeast)	lig4	NP_587888.2
Xenopus tropicalis (tropical clawed frog)	lig4	NP_001016981.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study also uncovered an important role for Rev3 and Rev7 (but not for Pol32) subunits of polymerase z in the survival of cells undergoing telomere losses and forming palindromes
Title: Telomere Dysfunction Triggers Palindrome Formation Independently of Double-Strand Break Repair Mechanisms.

Dnl4 BRCT1 domain supports DNA double-strand breaks recruitment and NHEJ in a manner distinct from Lif1 binding and reveal a complexity of Dnl4 BRCT domain functions in support of stable DNA double-strand breaks association.
Title: Yeast DNA ligase IV mutations reveal a nonhomologous end joining function of BRCT1 distinct from XRCC4/Lif1 binding.

These findings indicate that Dnl4 can promote mutagenic end joining independently of its catalytic activity, likely by a mechanism that involves Cdc9.
Title: Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity.

lack of DNL4 rescues the short chronologic life span of the yme1 mutant.
Title: Accumulation of linear mitochondrial DNA fragments in the nucleus shortens the chronological life span of yeast.

Consistently, dnl4 cells displayed altered fork structures at the replication fork barrier, leading us to propose an as yet unrecognized role for Dnl4 in the maintenance of ribosomal DNA stability.
Title: DNA ligase 4 stabilizes the ribosomal DNA array upon fork collapse at the replication fork barrier.

The following DNL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03320	NM_001183424.1 Latest version!	Saccharomyces cerevisiae S288C DNA ligase (ATP) DNL4 (DNL4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNL4 ( NM_001183424.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014647.1 Saccharomyces cerevisiae S288C DNA ligase (ATP) DNL4 (DNL4), partial mRNA.

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CloneID	OSi03320
Clone ID Related Accession (Same CDS sequence)	NM_001183424.1
Accession Version	NM_001183424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA ligase (ATP) DNL4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATATCAG CACTAGATTC TATACCCGAG CCCCAAAACT TTGCGCCTAG TCCAGATTTC 
AAATGGCTTT GTGAAGAGCT ATTTGTGAAG ATACATGAAG TTCAAATTAA TGGAACGGCC 
GGCACTGGCA AATCAAGGTC TTTCAAGTAC TATGAAATAA TATCGAATTT CGTCGAAATG 
TGGAGAAAAA CCGTGGGAAA TAATATATAT CCTGCACTGG TTCTTGCTCT TCCCTACCGC 
GATAGACGAA TCTATAATAT TAAGGATTAT GTATTAATAA GAACTATATG CTCTTACTTG 
AAGTTGCCAA AAAATTCTGC AACAGAGCAG CGGTTAAAAG ATTGGAAACA GCGTGTCGGT 
AAAGGTGGGA ATCTTTCTTC TCTTCTTGTG GAAGAAATTG CTAAAAGAAG GGCTGAACCT 
AGCTCAAAAG CGATTACAAT TGATAACGTC AATCACTATC TGGATAGTTT GAGTGGAGAC 
AGGTTCGCTT CCGGACGAGG ATTTAAGAGT CTTGTCAAGT CCAAACCTTT CCTGCACTGT 
GTGGAGAATA TGAGTTTCGT CGAATTAAAA TACTTCTTTG ATATCGTGCT TAAAAATAGA 
GTAATAGGAG GTCAAGAGCA CAAATTGCTA AACTGCTGGC ATCCTGATGC TCAGGATTAT 
CTTAGCGTGA TATCTGATTT AAAGGTGGTA ACTTCAAAAC TTTATGATCC AAAAGTTCGT 
CTAAAGGATG ATGATTTGAG TATAAAAGTT GGCTTTGCAT TCGCCCCCCA ATTAGCCAAA 
AAAGTGAATC TTTCTTATGA GAAAATATGC CGTACACTAC ATGATGATTT TTTGGTAGAA 
GAAAAAATGG ATGGAGAACG AATTCAAGTT CATTATATGA ATTATGGTGA ATCCATAAAA 
TTTTTTAGTA GACGGGGCAT CGACTATACC TATTTGTACG GAGCGAGCTT ATCATCAGGA 
ACTATATCTC AACATTTGAG GTTTACAGAT AGTGTTAAAG AATGTGTTTT AGATGGAGAA 
ATGGTGACGT TTGATGCAAA AAGACGGGTG ATTCTTCCAT TCGGTCTTGT TAAAGGAAGT 
GCAAAGGAAG CGCTATCTTT TAATAGTATA AATAATGTTG ACTTTCACCC CTTATATATG 
GTGTTTGATC TGTTATACCT GAATGGGACT TCGTTGACAC CATTACCCCT TCATCAAAGG 
AAGCAATATC TGAACAGCAT TTTAAGTCCC TTGAAAAATA TTGTAGAAAT AGTACGATCT 
TCTAGATGTT ATGGTGTGGA GTCAATCAAA AAGTCTTTAG AAGTTGCAAT CTCACTGGGT 
TCAGAAGGAG TTGTTTTGAA ATATTATAAT TCAAGTTATA ATGTCGCCAG TCGAAACAAC 
AACTGGATCA AGGTAAAACC TGAATATTTG GAGGAATTTG GAGAGAATTT AGACTTAATA 
GTAATAGGCA GAGATTCTGG GAAAAAAGAT TCTTTTATGC TAGGGTTACT TGTGCTAGAT 
GAAGAAGAGT ATAAAAAGCA CCAAGGAGAC TCCTCTGAAA TTGTAGACCA CTCAAGCCAA 
GAAAAACACA TACAAAATTC AAGAAGAAGG GTGAAAAAAA TACTTTCATT CTGTTCTATC 
GCAAACGGTA TATCTCAAGA AGAATTCAAA GAAATCGACC GCAAAACGAG AGGACATTGG 
AAAAGAACCT CCGAAGTTGC TCCCCCTGCT TCAATTTTAG AATTTGGCTC AAAAATACCT 
GCCGAATGGA TTGACCCCAG TGAATCAATT GTTCTAGAAA TAAAATCACG GTCTTTGGAT 
AACACAGAAA CGAATATGCA GAAGTACGCT ACCAATTGTA CTTTGTACGG TGGCTATTGT 
AAAAGAATAC GGTACGATAA AGAATGGACA GATTGTTACA CACTTAACGA CTTATACGAA 
AGTAGGACGG TTAAATCTAA CCCCAGCTAT CAAGCGGAAA GGTCACAGCT TGGATTGATA 
CGGAAAAAGA GAAAGAGAGT ACTTATTTCA GACAGCTTTC ACCAAAACAG GAAACAACTG 
CCAATTTCAA ACATCTTTGC CGGATTACTT TTTTATGTTC TCTCTGACTA TGTCACGGAG 
GACACTGGAA TACGGATTAC ACGGGCAGAA CTTGAAAAAA CTATTGTGGA ACATGGTGGT 
AAACTGATAT ATAATGTAAT TTTAAAACGT CATTCAATTG GGGACGTTCG GTTAATCAGC 
TGTAAAACTA CCACGGAATG CAAGGCTTTA ATAGATCGAG GATATGATAT ATTGCACCCA 
AATTGGGTAC TCGATTGTAT AGCATATAAG AGGCTCATCC TGATCGAGCC CAATTATTGC 
TTTAACGTCT CTCAAAAAAT GAGAGCCGTC GCTGAAAAAA GGGTAGATTG TTTGGGTGAT 
AGTTTTGAAA ATGACATTTC GGAAACCAAA CTGTCATCAT TGTATAAATC ACAACTAAGT 
CTACCACCGA TGGGGGAACT CGAGATAGAT TCTGAGGTTC GGCGGTTTCC ATTATTTTTA 
TTCTCCAACA GGATTGCATA CGTACCACGT CGCAAAATTA GCACAGAAGA TGACATTATA 
GAAATGAAAA TTAAGTTGTT TGGTGGAAAA ATAACAGATC AACAGTCACT TTGTAACTTA 
ATAATTATAC CATATACTGA TCCTATTTTG AGGAAAGACT GCATGAATGA GGTACACGAA 
AAAATAAAAG AACAAATAAA GGCTTCTGAT ACTATACCGA AAATAGCCAG GGTCGTTGCC 
CCTGAATGGG TGGATCATTC TATTAATGAA AACTGTCAAG TGCCTGAAGA AGACTTCCCC 
GTAGTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014647.1
CDS	1..2835
Translation	MISALDSIPEPQNFAPSPDFKWLCEELFVKIHEVQINGTAGTGKSRSFKYYEIISNFVEMWRKTVGNNIYPALVLALPYRDRRIYNIKDYVLIRTICSYLKLPKNSATEQRLKDWKQRVGKGGNLSSLLVEEIAKRRAEPSSKAITIDNVNHYLDSLSGDRFASGRGFKSLVKSKPFLHCVENMSFVELKYFFDIVLKNRVIGGQEHKLLNCWHPDAQDYLSVISDLKVVTSKLYDPKVRLKDDDLSIKVGFAFAPQLAKKVNLSYEKICRTLHDDFLVEEKMDGERIQVHYMNYGESIKFFSRRGIDYTYLYGASLSSGTISQHLRFTDSVKECVLDGEMVTFDAKRRVILPFGLVKGSAKEALSFNSINNVDFHPLYMVFDLLYLNGTSLTPLPLHQRKQYLNSILSPLKNIVEIVRSSRCYGVESIKKSLEVAISLGSEGVVLKYYNSSYNVASRNNNWIKVKPEYLEEFGENLDLIVIGRDSGKKDSFMLGLLVLDEEEYKKHQGDSSEIVDHSSQEKHIQNSRRRVKKILSFCSIANGISQEEFKEIDRKTRGHWKRTSEVAPPASILEFGSKIPAEWIDPSESIVLEIKSRSLDNTETNMQKYATNCTLYGGYCKRIRYDKEWTDCYTLNDLYESRTVKSNPSYQAERSQLGLIRKKRKRVLISDSFHQNRKQLPISNIFAGLLFYVLSDYVTEDTGIRITRAELEKTIVEHGGKLIYNVILKRHSIGDVRLISCKTTTECKALIDRGYDILHPNWVLDCIAYKRLILIEPNYCFNVSQKMRAVAEKRVDCLGDSFENDISETKLSSLYKSQLSLPPMGELEIDSEVRRFPLFLFSNRIAYVPRRKISTEDDIIEMKIKLFGGKITDQQSLCNLIIIPYTDPILRKDCMNEVHEKIKEQIKASDTIPKIARVVAPEWVDHSINENCQVPEEDFPVVNY

Target ORF information:

RefSeq Version	NM_001183424.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA ligase (ATP) DNL4 (DNL4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183424.1

ORF Insert Sequence:

ATGATATCAG CACTAGATTC TATACCCGAG CCCCAAAACT TTGCGCCTAG TCCAGATTTC 
AAATGGCTTT GTGAAGAGCT ATTTGTGAAG ATACATGAAG TTCAAATTAA TGGAACGGCC 
GGCACTGGCA AATCAAGGTC TTTCAAGTAC TATGAAATAA TATCGAATTT CGTCGAAATG 
TGGAGAAAAA CCGTGGGAAA TAATATATAT CCTGCACTGG TTCTTGCTCT TCCCTACCGC 
GATAGACGAA TCTATAATAT TAAGGATTAT GTATTAATAA GAACTATATG CTCTTACTTG 
AAGTTGCCAA AAAATTCTGC AACAGAGCAG CGGTTAAAAG ATTGGAAACA GCGTGTCGGT 
AAAGGTGGGA ATCTTTCTTC TCTTCTTGTG GAAGAAATTG CTAAAAGAAG GGCTGAACCT 
AGCTCAAAAG CGATTACAAT TGATAACGTC AATCACTATC TGGATAGTTT GAGTGGAGAC 
AGGTTCGCTT CCGGACGAGG ATTTAAGAGT CTTGTCAAGT CCAAACCTTT CCTGCACTGT 
GTGGAGAATA TGAGTTTCGT CGAATTAAAA TACTTCTTTG ATATCGTGCT TAAAAATAGA 
GTAATAGGAG GTCAAGAGCA CAAATTGCTA AACTGCTGGC ATCCTGATGC TCAGGATTAT 
CTTAGCGTGA TATCTGATTT AAAGGTGGTA ACTTCAAAAC TTTATGATCC AAAAGTTCGT 
CTAAAGGATG ATGATTTGAG TATAAAAGTT GGCTTTGCAT TCGCCCCCCA ATTAGCCAAA 
AAAGTGAATC TTTCTTATGA GAAAATATGC CGTACACTAC ATGATGATTT TTTGGTAGAA 
GAAAAAATGG ATGGAGAACG AATTCAAGTT CATTATATGA ATTATGGTGA ATCCATAAAA 
TTTTTTAGTA GACGGGGCAT CGACTATACC TATTTGTACG GAGCGAGCTT ATCATCAGGA 
ACTATATCTC AACATTTGAG GTTTACAGAT AGTGTTAAAG AATGTGTTTT AGATGGAGAA 
ATGGTGACGT TTGATGCAAA AAGACGGGTG ATTCTTCCAT TCGGTCTTGT TAAAGGAAGT 
GCAAAGGAAG CGCTATCTTT TAATAGTATA AATAATGTTG ACTTTCACCC CTTATATATG 
GTGTTTGATC TGTTATACCT GAATGGGACT TCGTTGACAC CATTACCCCT TCATCAAAGG 
AAGCAATATC TGAACAGCAT TTTAAGTCCC TTGAAAAATA TTGTAGAAAT AGTACGATCT 
TCTAGATGTT ATGGTGTGGA GTCAATCAAA AAGTCTTTAG AAGTTGCAAT CTCACTGGGT 
TCAGAAGGAG TTGTTTTGAA ATATTATAAT TCAAGTTATA ATGTCGCCAG TCGAAACAAC 
AACTGGATCA AGGTAAAACC TGAATATTTG GAGGAATTTG GAGAGAATTT AGACTTAATA 
GTAATAGGCA GAGATTCTGG GAAAAAAGAT TCTTTTATGC TAGGGTTACT TGTGCTAGAT 
GAAGAAGAGT ATAAAAAGCA CCAAGGAGAC TCCTCTGAAA TTGTAGACCA CTCAAGCCAA 
GAAAAACACA TACAAAATTC AAGAAGAAGG GTGAAAAAAA TACTTTCATT CTGTTCTATC 
GCAAACGGTA TATCTCAAGA AGAATTCAAA GAAATCGACC GCAAAACGAG AGGACATTGG 
AAAAGAACCT CCGAAGTTGC TCCCCCTGCT TCAATTTTAG AATTTGGCTC AAAAATACCT 
GCCGAATGGA TTGACCCCAG TGAATCAATT GTTCTAGAAA TAAAATCACG GTCTTTGGAT 
AACACAGAAA CGAATATGCA GAAGTACGCT ACCAATTGTA CTTTGTACGG TGGCTATTGT 
AAAAGAATAC GGTACGATAA AGAATGGACA GATTGTTACA CACTTAACGA CTTATACGAA 
AGTAGGACGG TTAAATCTAA CCCCAGCTAT CAAGCGGAAA GGTCACAGCT TGGATTGATA 
CGGAAAAAGA GAAAGAGAGT ACTTATTTCA GACAGCTTTC ACCAAAACAG GAAACAACTG 
CCAATTTCAA ACATCTTTGC CGGATTACTT TTTTATGTTC TCTCTGACTA TGTCACGGAG 
GACACTGGAA TACGGATTAC ACGGGCAGAA CTTGAAAAAA CTATTGTGGA ACATGGTGGT 
AAACTGATAT ATAATGTAAT TTTAAAACGT CATTCAATTG GGGACGTTCG GTTAATCAGC 
TGTAAAACTA CCACGGAATG CAAGGCTTTA ATAGATCGAG GATATGATAT ATTGCACCCA 
AATTGGGTAC TCGATTGTAT AGCATATAAG AGGCTCATCC TGATCGAGCC CAATTATTGC 
TTTAACGTCT CTCAAAAAAT GAGAGCCGTC GCTGAAAAAA GGGTAGATTG TTTGGGTGAT 
AGTTTTGAAA ATGACATTTC GGAAACCAAA CTGTCATCAT TGTATAAATC ACAACTAAGT 
CTACCACCGA TGGGGGAACT CGAGATAGAT TCTGAGGTTC GGCGGTTTCC ATTATTTTTA 
TTCTCCAACA GGATTGCATA CGTACCACGT CGCAAAATTA GCACAGAAGA TGACATTATA 
GAAATGAAAA TTAAGTTGTT TGGTGGAAAA ATAACAGATC AACAGTCACT TTGTAACTTA 
ATAATTATAC CATATACTGA TCCTATTTTG AGGAAAGACT GCATGAATGA GGTACACGAA 
AAAATAAAAG AACAAATAAA GGCTTCTGAT ACTATACCGA AAATAGCCAG GGTCGTTGCC 
CCTGAATGGG TGGATCATTC TATTAATGAA AACTGTCAAG TGCCTGAAGA AGACTTCCCC 
GTAGTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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DNL4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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