IRA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	IRA2
Entrez Gene ID	854073
Full Name	Ras GTPase activating protein IRA2
Synonyms	CCS1,GLC4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183335.1	NP_014560.1	Ras GTPase activating protein IRA2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	IRA1	NP_009698.3
Eremothecium gossypii	AGOS_AER025C	NP_984885.2
Saccharomyces cerevisiae (baker's yeast)	IRA2	NP_014560.1
Kluyveromyces lactis	KLLA0E17887g	XP_454757.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IRA2 mutation reveals the phenotypic effects of the cryptic variants by disrupting the transcriptional silencing of one or more genes that contribute to the trait.We also show that this ability to disrupt FLO11 repression is not unique to IRA2
Title: Transcriptional Derepression Uncovers Cryptic Higher-Order Genetic Interactions.

the physiological relevance of the interaction of both Ira2 and Hsp60 with Bcy1. Bcy1 interacts with Ira2 tethering PKA to the Ras complex and Hsp60 chaperone localizes PKA to mitochondria and has a role in the kinase stability.
Title: Interacting proteins of protein kinase A regulatory subunit in Saccharomyces cerevisiae.

particular, the effect of the negative feedback loops on the activity of both Ira2 (a Ras-GAP) and Cdc25 (a Ras-GEF) proteins.
Title: Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states.

These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 (in yeast) and neurofibromin (in humans).
Title: The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans.

Ira1 and Ira2 affect glycerophosphoinositol production and transport.
Title: Neurofibromin homologs Ira1 and Ira2 affect glycerophosphoinositol production and transport in Saccharomyces cerevisiae.

The following IRA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03239	NM_001183335.1 Latest version!	Saccharomyces cerevisiae S288C Ras GTPase activating protein IRA2 (IRA2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRA2 ( NM_001183335.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014560.1 Saccharomyces cerevisiae S288C Ras GTPase activating protein IRA2 (IRA2), partial mRNA.

Price & Availability↑

CloneID	OSi03239
Clone ID Related Accession (Same CDS sequence)	NM_001183335.1
Accession Version	NM_001183335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ras GTPase activating protein IRA2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCAGC CCACTAAGAA TAAGAAGAAA GAACACGGGA CCGATTCCAA GTCATCCCGC 
ATGACTCGGA CGTTGGTTAA TCATATTCTT TTTGAAAGAA TTCTCCCGAT CCTTCCGGTG 
GAGTCTAATC TAAGTACCTA TTCGGAAGTG GAAGAGTATT CCTCATTCAT TTCATGCAGA 
TCTGTGCTCA TTAACGTTAC CGTTTCCCGA GATGCAAATG CTATGGTGGA AGGCACCTTG 
GAGTTGATAG AATCGCTTCT TCAAGGGCAC GAAATCATTT CAGATAAGGG TAGCAGTGAC 
GTTATTGAAT CAATACTGAT TATACTAAGA TTGTTAAGTG ATGCGCTAGA GTATAATTGG 
CAAAATCAAG AAAGCCTTCA TTACAACGAC ATTTCGACTC ACGTAGAACA TGACCAAGAA 
CAGAAGTACA GACCAAAGCT TAACAGTATT CTGCCCGACT ACTCGTCGAC TCATTCCAAT 
GGCAACAAAC ACTTTTTCCA CCAGAGCAAA CCTCAGGCAC TGATACCGGA ACTGGCATCG 
AAATTGCTTG AGAGTTGCGC GAAGTTGAAG TTCAATACAA GAACTTTGCA AATTTTACAA 
AATATGATCA GTCATGTTCA TGGAAACATT CTAACGACTT TGAGTTCCTC GATTCTTCCC 
CGCCACAAAT CCTATCTGAC AAGGCACAAC CATCCTTCTC ATTGTAAAAT GATTGACTCT 
ACTCTAGGCC ATATTCTCCG ATTTGTAGCG GCTTCCAATC CGTCCGAGTA TTTTGAATTT 
ATCAGAAAGA GTGTGCAAGT GCCCGTAACA CAGACACACA CGCATTCACA CTCCCATTCA 
CACTCTTTGC CATCTTCCGT TTATAACAGC ATAGTGCCCC ACTTTGATCT TTTCAGCTTC 
ATCTATTTAA GCAAGCATAA TTTTAAGAAA TACTTGGAAC TCATCAAAAA CTTATCGGTG 
ACGTTAAGGA AAACGATTTA TCATTGCCTA CTTTTGCATT ACAGCGCCAA AGCAATAATG 
TTTTGGATAA TGGCTAGGCC TGCGGAATAT TATGAACTCT TCAACTTATT AAAAGATAAT 
AACAATGAAC ACTCGAAATC CTTAAACACG TTAAACCATA CACTTTTCGA GGAGATCCAT 
TCGACTTTTA ATGTGAATAG CATGATAACC ACCAATCAAA ATGCTCATCA AGGCTCATCT 
TCCCCTTCGT CCTCCTCGCC ATCGTCACCA CCTAGCTCAT CATCATCGGA TAACAACAAT 
CAAAACATAA TAGCAAAATC CTTAAGTCGT CAGCTTTCTC ACCACCAGTC ATACATTCAA 
CAGCAGTCTG AAAGAAAACT ACATTCTTCA TGGACTACAA ACTCTCAATC CTCTACTTCA 
CTGTCATCTT CAACGTCTAA TTCAACAACA ACTGATTTCT CTACTCACAC TCAACCAGGA 
GAATATGACC CTTCCTTACC AGATACTCCC ACGATGTCTA ACATCACTAT TAGTGCATCT 
TCATTATTAT CTCAAACTCC AACTCCAACA ACACAATTGC AACAGCGGTT GAACTCAGCA 
GCTGCAGCCG CCGCCGCAGC TGCTTCACCA TCGAATTCCA CCCCAACTGG ATACACAGCA 
GAGCAACAAA GTCGCGCTTC ATACGATGCA CACAAAACTG GCCATACTGG TAAGGATTAT 
GACGAACATT TTTTGTCTGT CACTCGTTTG GATAATGTTT TGGAGTTATA CACGCACTTT 
GATGATACTG AGGTACTACC ACACACATCC GTACTGAAGT TTTTAACTAC TTTGACAATG 
TTCGATATTG ACCTTTTTAA TGAATTAAAC GCTACATCAT TCAAATATAT TCCTGATTGT 
ACTATGCATC GTCCAAAAGA AAGAACAAGT TCTTTCAATA ATACTGCACA CGAGACAGGT 
TCCGAAAAGA CTTCGGGTAT AAAACATATT ACACAAGGCT TAAAGAAATT AACTTCTTTA 
CCTTCCTCAA CCAAAAAAAC TGTAAAATTT GTGAAGATGT TGCTAAGAAA TTTAAATGGG 
AATCAAGCTG TATCAGATGT TGCCCTCTTA GATACAATGA GGGCCTTACT ATCATTCTTT 
ACAATGACTT CTGCGGTCTT TCTCGTGGAT AGAAACTTAC CCTCAGTACT TTTTGCCAAG 
AGACTCATCC CCATAATGGG GACAAATTTA AGCGTCGGTC AAGACTGGAA TTCAAAAATA 
AATAACAGTT TGATGGTTTG TTTGAAAAAA AACTCCACCA CGTTTGTTCA ATTACAATTA 
ATATTCTTCT CTTCAGCTAT TCAATTCGAT CATGAATTAT TACTGGCACG TCTGAGCATC 
GATACAATGG CCAACAATTT AAACATGCAG AAGCTATGCC TTTATACTGA AGGATTCAGG 
ATATTCTTCG ACATACCAAG TAAGAAGGAA TTGCGGAAGG CAATTGCGGT TAAAATTTCT 
AAATTTTTCA AAACATTATT CTCCATTATA GCAGATATTC TTTTACAAGA ATTTCCGTAT 
TTTGATGAGC AAATCACCGA CATAGTTGCT TCCATTCTTG ACGGTACAAT TATCAATGAG 
TATGGTACGA AGAAACATTT CAAGGGGAGC TCACCCTCTT TATGTTCGAC AACCCGGTCA 
AGATCAGGAT CTACATCTCA AAGTTCAATG ACACCAGTTT CTCCGCTGGG ACTGGATACT 
GATATATGTC CAATGAACAC CCTGTCTTTA GTTGGTTCAA GTACTTCAAG AAATTCTGAC 
AACGTTAATT CATTAAACAG TTCACCAAAG AACTTGTCTT CTGATCCATA CTTGTCACAT 
CTTGTGGCCC CAAGAGCGCG TCATGCTTTA GGTGGGCCAT CTAGTATTAT AAGGAATAAA 
ATACCGACTA CATTGACTTC ACCTCCAGGA ACGGAAAAAT CTTCACCAGT ACAACGTCCG 
CAAACGGAAA GCATCAGTGC CACACCAATG GCCATAACAA ATTCTACTCC ATTATCGTCG 
GCAGCATTCG GAATTCGATC GCCTTTGCAG AAAATAAGAA CGAGGCGTTA TTCCGATGAA 
AGTTTAGGAA AATTCATGAA ATCAACAAAT AATTACATTC AAGAACATTT GATACCAAAA 
GATTTGAATG AAGCAACTCT TCAAGATGCT AGAAGAATAA TGATTAATAT TTTCAGTATT 
TTTAAGAGAC CGAATAGTTA CTTCATCATT CCTCACAATA TAAACTCGAA TTTACAATGG 
GTTTCGCAGG ATTTTAGAAA TATTATGAAA CCGATTTTCG TCGCCATCGT AAGTCCGGAT 
GTAGATTTAC AGAATACTGC TCAATCATTC ATGGATACCT TATTATCGAA TGTTATTACT 
TATGGTGAAT CAGATGAGAA TATCAGTATT GAAGGGTATC ATCTTCTTTG CAGTTACACT 
GTAACATTAT TTGCAATGGG CCTTTTCGAT TTGAAAATTA ATAATGAAAA GCGTCAAATT 
CTCTTGGATA TAACTGTCAA GTTTATGAAG GTTAGATCAC ATTTAGCAGG GATCGCGGAG 
GCCTCACACC ACATGGAATA CATAAGTGAT TCTGAAAAAC TCACCTTTCC GCTGATTATG 
GGGACTGTTG GTAGGGCCCT ATTTGTTTCA TTATACTCTA GTCAACAAAA AATTGAAAAG 
ACTTTAAAGA TTGCTTACAC AGAGTATCTT TCTGCAATCA ATTTTCATGA GAGGAATATT 
GATGATGCTG ATAAAACTTG GGTTCATAAT ATTGAGTTTG TAGAAGCGAT GTGTCATGAC 
AACTACACAA CTTCTGGTTC AATTGCTTTC CAAAGGAGGA CAAGAAATAA TATTTTACGA 
TTTGCTACTA TTCCTAACGC TATCTTACTT GATTCTATGA GGATGATCTA TAAGAAGTGG 
CATACTTACA CACACAGTAA AAGTTTAGAA AAACAAGAAC GGAACGACTT CAGAAATTTC 
GCGGGTATTT TAGCCTCTTT GTCGGGTATC CTATTCATCA ATAAAAAGAT ATTGCAAGAA 
ATGTATCCAT ACCTACTCGA CACCGTTTCA GAATTGAAAA AAAATATAGA CTCTTTTATC 
TCAAAACAAT GCCAATGGTT AAACTATCCG GATTTATTAA CGAGAGAAAA TTCAAGAGAT 
ATTCTAAGTG TAGAACTGCA TCCTTTGTCT TTTAACTTAC TTTTTAATAA TTTGAGGCTC 
AAGTTAAAAG AACTTGCTTG TTCAGACTTA TCAATACCAG AAAATGAAAG TTCCTATGTT 
TTATTAGAAC AAATAATCAA AATGCTGCGG ACAATCCTAG GTCGTGATGA TGACAATTAT 
GTAATGATGC TTTTTTCCAC AGAGATTGTA GATCTTATTG ATTTATTGAC AGATGAAATA 
AAAAAAATAC CAGCCTATTG TCCAAAATAT CTCAAGGCAA TTATTCAAAT GACCAAAATG 
TTCAGTGCCT TGCAGCACTC AGAGGTTAAT TTAGGTGTCA AAAATCATTT TCACGTTAAA 
AATAAATGGT TGAGGCAAAT CACTGATTGG TTTCAAGTGA GTATTGCGAG AGAGTACGAT 
TTCGAAAACT TGTCAAAACC TCTAAAAGAA ATGGATTTGG TAAAAAGAGA CATGGATATT 
CTATACATAG ATACGGCAAT CGAAGCTTCA ACCGCTATTG CGTACCTCAC GAGACATACT 
TTCTTAGAGA TTCCACCTGC CGCGTCAGAT CCCGAACTAT CTCGATCTAG GTCTGTGATA 
TTTGGGTTTT ATTTCAACAT CTTAATGAAA GGCCTTGAAA AAAGTAGTGA TCGTGACAAT 
TACCCAGTAT TCTTGAGGCA CAAAATGAGT GTCCTCAACG ACAATGTAAT ACTTTCATTA 
ACAAATCTTT CAAACACCAA TGTTGATGCG AGTTTGCAGT TCACCTTACC GATGGGCTAT 
TCCGGAAATC GAAACATTAG GAATGCATTT TTGGAGGTCT TCATTAATAT CGTTACGAAC 
TATCGGACAT ACACGGCTAA AACTGACCTT GGAAAATTAG AGGCAGCAGA CAAATTTTTG 
CGATATACGA TTGAACATCC CCAGCTATCG TCCTTTGGAG CAGCGGTTTG TCCCGCTAGC 
GATATTGATG CTTATGCTGC TGGCTTAATA AATGCATTTG AAACGAGGAA TGCCACCCAC 
ATTGTAGTGG CACAGTTGAT TAAAAATGAA ATTGAAAAAT CTTCCAGACC TACGGATATC 
CTTAGAAGAA ATAGCTGTGC TACGAGATCA TTATCTATGC TAGCCAGGTC CAAGGGTAAC 
GAATATTTGA TTCGCACTTT GCAACCATTA CTAAAAAAAA TTATCCAGAA CAGAGATTTT 
TTTGAAATTG AGAAACTAAA ACCGGAAGAT TCAGATGCTG AACGTCAAAT AGAGCTCTTC 
GTTAAATACA TGAATGAATT ATTGGAATCC ATATCCAACT CCGTATCTTA TTTTCCCCCT 
CCTTTATTTT ATATTTGCCA AAACATTTAT AAAGTTGCGT GTGAAAAATT TCCGGATCAC 
GCAATTATCG CCGCTGGGTC TTTCGTGTTT TTACGGTTTT TTTGTCCTGC TTTAGTCAGC 
CCTGATTCTG AAAATATCAT AGATATTTCT CACTTGAGCG AAAAGCGTAC CTTCATCAGC 
TTGGCTAAAG TTATCCAAAA TATTGCCAAT GGCTCAGAAA ATTTCTCCAG ATGGCCAGCT 
TTGTGTTCCC AAAAGGATTT TCTTAAGGAA TGTAGCGATA GAATTTTCAG ATTCCTAGCT 
GAACTTTGTA GAACAGATCG CACGATAGAC ATCCAAGTGA GAACAGACCC AACGCCAATT 
GCATTTGACT ATCAATTCCT TCATTCCTTT GTTTACCTTT ACGGTCTTGA GGTGAGAAGG 
AATGTGCTAA ATGAAGCAAA ACATGATGAT GGTGACATTG ATGGTGACGA TTTCTATAAG 
ACCACATTTT TACTTATTGA TGATGTTCTT GGCCAATTAG GCCAACCTAA AATGGAATTT 
TCCAATGAAA TACCAATATA CATAAGAGAA CATATGGACG ACTATCCGGA ACTGTATGAG 
TTCATGAATA GGCACGCGTT CAGAAACATT GAGACTTCAA CAGCGTACAG CCCAAGCGTT 
CACGAGTCCA CCTCAAGTGA AGGCATTCCA ATTATTACGT TAACAATGTC AAATTTCTCA 
GACAGACATG TGGACATTGA TACAGTTGCT TACAAGTTCT TGCAAATTTA TGCTCGAATC 
TGGACCACCA AACACTGTTT AATAATCGAC TGTACAGAAT TTGACGAGGG AGGGCTTGAT 
ATGAGGAAAT TTATTTCTTT GGTTATGGGA CTATTACCAG AAGTTGCACC CAAAAATTGT 
ATAGGCTGTT ACTACTTTAA CGTAAACGAG ACATTTATGG ATAATTATGG AAAATGTTTG 
GACAAAGACA ACGTATATGT TTCCTCGAAA ATTCCTCATT ATTTCATTAA TAGTAACTCT 
GATGAAGGAC TTATGAAATC TGTGGGTATA ACTGGACAAG GGTTGAAGGT TCTGCAAGAT 
ATTCGTGTCT CTCTGCATGA TATCACGCTT TATGACGAAA AAAGAAATAG ATTTACGCCG 
GTATCGTTGA AAATAGGCGA TATTTACTTT CAAGTCTTGC ATGAAACTCC TAGGCAATAT 
AAAATAAGGG ACATGGGTAC TTTATTCGAC GTAAAATTCA ATGATGTCTA CGAAATTAGC 
CGAATATTTG AAGTACATGT TTCGTCAATA ACTGGAGTGG CAGCTGAATT TACAGTAACT 
TTTCAGGACG AGAGAAGGTT GATTTTTAGT AGTCCGAAAT ACCTTGAAAT TGTGAAGATG 
TTCTATTACG CACAGATCCG GTTAGAAAGT GAATATGAAA TGGATAATAA TTCGAGTACC 
TCCTCCCCAA ATTCAAACAA CAAGGACAAA CAGCAGAAAG AGAGAACAAA ACTATTGTGC 
CACCTACTGT TAGTATCTCT TATTGGTCTG TTTGATGAGA GTAAAAAAAT GAAAAACAGT 
TCGTATAACC TAATAGCTGC CACTGAGGCG TCATTTGGTT TGAACTTTGG CTCCCATTTT 
CATCGCTCTC CCGAGGTGTA CGTCCCCGAA GATACTACAA CATTTTTAGG TGTTATTGGA 
AAGTCTCTTG CAGAGTCTAA TCCAGAACTC ACAGCCTATA TGTTTATCTA TGTTTTGGAG 
GCATTGAAGA ACAACGTAAT TCCTCACGTT TACATCCCTC ATACCATTTG CGGTTTGTCT 
TATTGGATCC CTAATTTATA CCAACATGTG TATTTGGCTG ATGATGAAGA AGGCCCCGAA 
AACATATCTC ACATTTTCCG AATTCTTATC AGGCTCTCTG TGAGAGAGAC TGACTTTAAA 
GCCGTATACA TGCAATATGT TTGGTTGCTA CTTTTAGATG ATGGCCGCTT AACTGACATT 
ATCGTTGATG AAGTTATTAA TCATGCGTTA GAAAGAGACT CCGAAAACCG CGATTGGAAG 
AAAACAATAT CGTTACTGAC TGTCCTACCC ACTACTGAGG TTGCTAATAA TATTATTCAA 
AAAATATTGG CAAAAATTAG ATCATTTTTA CCGTCATTGA AGTTAGAAGC TATGACCCAA 
AGTTGGTCTG AACTAACAAT ATTAGTTAAG ATAAGCATCC ACGTTTTTTT TGAAACTTCT 
TTGCTGGTAC AGATGTACTT ACCAGAGATC CTGTTTATCG TATCCTTATT AATTGATGTT 
GGTCCAAGGG AACTCAGATC ATCACTACAC CAGCTATTAA TGAATGTATG CCATTCCTTG 
GCTATTAACT CAGCTTTACC ACAAGATCAT AGAAATAATC TAGATGAAAT AAGTGATATA 
TTTGCACATC AAAAGGTGAA GTTTATGTTT GGGTTCAGCG AGGACAAAGG ACGAATTTTA 
CAGATTTTTA GCGCTTCTTC TTTTGCAAGC AAGTTTAATA TTCTGGATTT CTTCATCAAT 
AATATATTAT TGCTGATGGA ATATTCTTCA ACGTACGAAG CAAACGTGTG GAAGACAAGA 
TACAAGAAAT ATGTCTTGGA ATCTGTGTTT ACAAGTAATT CTTTTCTTTC GGCACGTTCA 
ATCATGATTG TTGGTATAAT GGGTAAATCT TACATAACTG AAGGGTTATG CAAGGCTATG 
TTAATTGAAA CCATGAAAGT TATCGCCGAA CCAAAGATTA CTGACGAGCA TCTTTTCTTA 
GCCATATCTC ATATTTTTAC TTATTCCAAA ATTGTTGAAG GTTTGGATCC CAACCTTGAC 
TTAATGAAGC ACTTATTTTG GTTTTCAACA CTCTTCCTTG AATCACGTCA CCCGATAATT 
TTTGAGGGTG CCCTTCTCTT TGTGTCAAAC TGTATAAGGC GCCTATACAT GGCCCAGTTT 
GAAAATGAAA GCGAAACATC ATTGATAAGT ACTTTACTTA AGGGGAGAAA GTTTGCTCAT 
ACCTTTTTAA GCAAGATAGA GAATCTTAGT GGTATTGTTT GGAATGAAGA TAATTTTACA 
CACATTCTGA TTTTCATCAT TAATAAAGGA CTATCCAATC CTTTCATTAA GAGTACGGCT 
TTTGATTTCT TGAAGATGAT GTTTAGAAAC TCCTACTTTG AGCATCAAAT CAATCAGAAA 
TCTGATCATT ATTTGTGCTA TATGTTCCTA TTGTATTTTG TTTTAAACTG CAATCAATTT 
GAGGAACTTT TAGGTGACGT TGATTTTGAA GGAGAAATGG TTAACATTGA AAACAAGAAC 
ACCATTCCTA AAATTTTGTT AGAGTGGTTG AGTTCGGATA ACGAAAATGC AAACATTACC 
CTCTATCAAG GTGCGATACT GTTCAAATGT TCAGTTACGG ATGAACCAAG TAGATTTAGG 
TTTGCGTTGA TTATTAGGCA TCTATTGACA AAGAAACCCA TTTGTGCATT GCGTTTTTAC 
AGTGTTATTC GTAACGAAAT AAGAAAAATA TCAGCATTTG AGCAAAATTC GGATTGTGTT 
CCACTTGCTT TCGATATTTT AAACTTATTA GTGACGCATT CAGAGTCTAA TTCGTTAGAA 
AAACTTCACG AAGAATCCAT TGAACGTCTA ACCAAAAGAG GTTTATCGAT TGTGACTTCT 
TCTGGTATAT TTGCGAAGAA CTCCGACATG ATGATACCTT TAGATGTAAA ACCTGAAGAT 
ATCTATGAAC GTAAGAGAAT AATGACAATG ATTTTATCAA GGATGTCATG TTCTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014560.1
CDS	1..9240
Translation	MSQPTKNKKKEHGTDSKSSRMTRTLVNHILFERILPILPVESNLSTYSEVEEYSSFISCRSVLINVTVSRDANAMVEGTLELIESLLQGHEIISDKGSSDVIESILIILRLLSDALEYNWQNQESLHYNDISTHVEHDQEQKYRPKLNSILPDYSSTHSNGNKHFFHQSKPQALIPELASKLLESCAKLKFNTRTLQILQNMISHVHGNILTTLSSSILPRHKSYLTRHNHPSHCKMIDSTLGHILRFVAASNPSEYFEFIRKSVQVPVTQTHTHSHSHSHSLPSSVYNSIVPHFDLFSFIYLSKHNFKKYLELIKNLSVTLRKTIYHCLLLHYSAKAIMFWIMARPAEYYELFNLLKDNNNEHSKSLNTLNHTLFEEIHSTFNVNSMITTNQNAHQGSSSPSSSSPSSPPSSSSSDNNNQNIIAKSLSRQLSHHQSYIQQQSERKLHSSWTTNSQSSTSLSSSTSNSTTTDFSTHTQPGEYDPSLPDTPTMSNITISASSLLSQTPTPTTQLQQRLNSAAAAAAAAASPSNSTPTGYTAEQQSRASYDAHKTGHTGKDYDEHFLSVTRLDNVLELYTHFDDTEVLPHTSVLKFLTTLTMFDIDLFNELNATSFKYIPDCTMHRPKERTSSFNNTAHETGSEKTSGIKHITQGLKKLTSLPSSTKKTVKFVKMLLRNLNGNQAVSDVALLDTMRALLSFFTMTSAVFLVDRNLPSVLFAKRLIPIMGTNLSVGQDWNSKINNSLMVCLKKNSTTFVQLQLIFFSSAIQFDHELLLARLSIDTMANNLNMQKLCLYTEGFRIFFDIPSKKELRKAIAVKISKFFKTLFSIIADILLQEFPYFDEQITDIVASILDGTIINEYGTKKHFKGSSPSLCSTTRSRSGSTSQSSMTPVSPLGLDTDICPMNTLSLVGSSTSRNSDNVNSLNSSPKNLSSDPYLSHLVAPRARHALGGPSSIIRNKIPTTLTSPPGTEKSSPVQRPQTESISATPMAITNSTPLSSAAFGIRSPLQKIRTRRYSDESLGKFMKSTNNYIQEHLIPKDLNEATLQDARRIMINIFSIFKRPNSYFIIPHNINSNLQWVSQDFRNIMKPIFVAIVSPDVDLQNTAQSFMDTLLSNVITYGESDENISIEGYHLLCSYTVTLFAMGLFDLKINNEKRQILLDITVKFMKVRSHLAGIAEASHHMEYISDSEKLTFPLIMGTVGRALFVSLYSSQQKIEKTLKIAYTEYLSAINFHERNIDDADKTWVHNIEFVEAMCHDNYTTSGSIAFQRRTRNNILRFATIPNAILLDSMRMIYKKWHTYTHSKSLEKQERNDFRNFAGILASLSGILFINKKILQEMYPYLLDTVSELKKNIDSFISKQCQWLNYPDLLTRENSRDILSVELHPLSFNLLFNNLRLKLKELACSDLSIPENESSYVLLEQIIKMLRTILGRDDDNYVMMLFSTEIVDLIDLLTDEIKKIPAYCPKYLKAIIQMTKMFSALQHSEVNLGVKNHFHVKNKWLRQITDWFQVSIAREYDFENLSKPLKEMDLVKRDMDILYIDTAIEASTAIAYLTRHTFLEIPPAASDPELSRSRSVIFGFYFNILMKGLEKSSDRDNYPVFLRHKMSVLNDNVILSLTNLSNTNVDASLQFTLPMGYSGNRNIRNAFLEVFINIVTNYRTYTAKTDLGKLEAADKFLRYTIEHPQLSSFGAAVCPASDIDAYAAGLINAFETRNATHIVVAQLIKNEIEKSSRPTDILRRNSCATRSLSMLARSKGNEYLIRTLQPLLKKIIQNRDFFEIEKLKPEDSDAERQIELFVKYMNELLESISNSVSYFPPPLFYICQNIYKVACEKFPDHAIIAAGSFVFLRFFCPALVSPDSENIIDISHLSEKRTFISLAKVIQNIANGSENFSRWPALCSQKDFLKECSDRIFRFLAELCRTDRTIDIQVRTDPTPIAFDYQFLHSFVYLYGLEVRRNVLNEAKHDDGDIDGDDFYKTTFLLIDDVLGQLGQPKMEFSNEIPIYIREHMDDYPELYEFMNRHAFRNIETSTAYSPSVHESTSSEGIPIITLTMSNFSDRHVDIDTVAYKFLQIYARIWTTKHCLIIDCTEFDEGGLDMRKFISLVMGLLPEVAPKNCIGCYYFNVNETFMDNYGKCLDKDNVYVSSKIPHYFINSNSDEGLMKSVGITGQGLKVLQDIRVSLHDITLYDEKRNRFTPVSLKIGDIYFQVLHETPRQYKIRDMGTLFDVKFNDVYEISRIFEVHVSSITGVAAEFTVTFQDERRLIFSSPKYLEIVKMFYYAQIRLESEYEMDNNSSTSSPNSNNKDKQQKERTKLLCHLLLVSLIGLFDESKKMKNSSYNLIAATEASFGLNFGSHFHRSPEVYVPEDTTTFLGVIGKSLAESNPELTAYMFIYVLEALKNNVIPHVYIPHTICGLSYWIPNLYQHVYLADDEEGPENISHIFRILIRLSVRETDFKAVYMQYVWLLLLDDGRLTDIIVDEVINHALERDSENRDWKKTISLLTVLPTTEVANNIIQKILAKIRSFLPSLKLEAMTQSWSELTILVKISIHVFFETSLLVQMYLPEILFIVSLLIDVGPRELRSSLHQLLMNVCHSLAINSALPQDHRNNLDEISDIFAHQKVKFMFGFSEDKGRILQIFSASSFASKFNILDFFINNILLLMEYSSTYEANVWKTRYKKYVLESVFTSNSFLSARSIMIVGIMGKSYITEGLCKAMLIETMKVIAEPKITDEHLFLAISHIFTYSKIVEGLDPNLDLMKHLFWFSTLFLESRHPIIFEGALLFVSNCIRRLYMAQFENESETSLISTLLKGRKFAHTFLSKIENLSGIVWNEDNFTHILIFIINKGLSNPFIKSTAFDFLKMMFRNSYFEHQINQKSDHYLCYMFLLYFVLNCNQFEELLGDVDFEGEMVNIENKNTIPKILLEWLSSDNENANITLYQGAILFKCSVTDEPSRFRFALIIRHLLTKKPICALRFYSVIRNEIRKISAFEQNSDCVPLAFDILNLLVTHSESNSLEKLHEESIERLTKRGLSIVTSSGIFAKNSDMMIPLDVKPEDIYERKRIMTMILSRMSCSA

Target ORF information:

RefSeq Version	NM_001183335.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ras GTPase activating protein IRA2 (IRA2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183335.1

ORF Insert Sequence:

ATGTCCCAGC CCACTAAGAA TAAGAAGAAA GAACACGGGA CCGATTCCAA GTCATCCCGC 
ATGACTCGGA CGTTGGTTAA TCATATTCTT TTTGAAAGAA TTCTCCCGAT CCTTCCGGTG 
GAGTCTAATC TAAGTACCTA TTCGGAAGTG GAAGAGTATT CCTCATTCAT TTCATGCAGA 
TCTGTGCTCA TTAACGTTAC CGTTTCCCGA GATGCAAATG CTATGGTGGA AGGCACCTTG 
GAGTTGATAG AATCGCTTCT TCAAGGGCAC GAAATCATTT CAGATAAGGG TAGCAGTGAC 
GTTATTGAAT CAATACTGAT TATACTAAGA TTGTTAAGTG ATGCGCTAGA GTATAATTGG 
CAAAATCAAG AAAGCCTTCA TTACAACGAC ATTTCGACTC ACGTAGAACA TGACCAAGAA 
CAGAAGTACA GACCAAAGCT TAACAGTATT CTGCCCGACT ACTCGTCGAC TCATTCCAAT 
GGCAACAAAC ACTTTTTCCA CCAGAGCAAA CCTCAGGCAC TGATACCGGA ACTGGCATCG 
AAATTGCTTG AGAGTTGCGC GAAGTTGAAG TTCAATACAA GAACTTTGCA AATTTTACAA 
AATATGATCA GTCATGTTCA TGGAAACATT CTAACGACTT TGAGTTCCTC GATTCTTCCC 
CGCCACAAAT CCTATCTGAC AAGGCACAAC CATCCTTCTC ATTGTAAAAT GATTGACTCT 
ACTCTAGGCC ATATTCTCCG ATTTGTAGCG GCTTCCAATC CGTCCGAGTA TTTTGAATTT 
ATCAGAAAGA GTGTGCAAGT GCCCGTAACA CAGACACACA CGCATTCACA CTCCCATTCA 
CACTCTTTGC CATCTTCCGT TTATAACAGC ATAGTGCCCC ACTTTGATCT TTTCAGCTTC 
ATCTATTTAA GCAAGCATAA TTTTAAGAAA TACTTGGAAC TCATCAAAAA CTTATCGGTG 
ACGTTAAGGA AAACGATTTA TCATTGCCTA CTTTTGCATT ACAGCGCCAA AGCAATAATG 
TTTTGGATAA TGGCTAGGCC TGCGGAATAT TATGAACTCT TCAACTTATT AAAAGATAAT 
AACAATGAAC ACTCGAAATC CTTAAACACG TTAAACCATA CACTTTTCGA GGAGATCCAT 
TCGACTTTTA ATGTGAATAG CATGATAACC ACCAATCAAA ATGCTCATCA AGGCTCATCT 
TCCCCTTCGT CCTCCTCGCC ATCGTCACCA CCTAGCTCAT CATCATCGGA TAACAACAAT 
CAAAACATAA TAGCAAAATC CTTAAGTCGT CAGCTTTCTC ACCACCAGTC ATACATTCAA 
CAGCAGTCTG AAAGAAAACT ACATTCTTCA TGGACTACAA ACTCTCAATC CTCTACTTCA 
CTGTCATCTT CAACGTCTAA TTCAACAACA ACTGATTTCT CTACTCACAC TCAACCAGGA 
GAATATGACC CTTCCTTACC AGATACTCCC ACGATGTCTA ACATCACTAT TAGTGCATCT 
TCATTATTAT CTCAAACTCC AACTCCAACA ACACAATTGC AACAGCGGTT GAACTCAGCA 
GCTGCAGCCG CCGCCGCAGC TGCTTCACCA TCGAATTCCA CCCCAACTGG ATACACAGCA 
GAGCAACAAA GTCGCGCTTC ATACGATGCA CACAAAACTG GCCATACTGG TAAGGATTAT 
GACGAACATT TTTTGTCTGT CACTCGTTTG GATAATGTTT TGGAGTTATA CACGCACTTT 
GATGATACTG AGGTACTACC ACACACATCC GTACTGAAGT TTTTAACTAC TTTGACAATG 
TTCGATATTG ACCTTTTTAA TGAATTAAAC GCTACATCAT TCAAATATAT TCCTGATTGT 
ACTATGCATC GTCCAAAAGA AAGAACAAGT TCTTTCAATA ATACTGCACA CGAGACAGGT 
TCCGAAAAGA CTTCGGGTAT AAAACATATT ACACAAGGCT TAAAGAAATT AACTTCTTTA 
CCTTCCTCAA CCAAAAAAAC TGTAAAATTT GTGAAGATGT TGCTAAGAAA TTTAAATGGG 
AATCAAGCTG TATCAGATGT TGCCCTCTTA GATACAATGA GGGCCTTACT ATCATTCTTT 
ACAATGACTT CTGCGGTCTT TCTCGTGGAT AGAAACTTAC CCTCAGTACT TTTTGCCAAG 
AGACTCATCC CCATAATGGG GACAAATTTA AGCGTCGGTC AAGACTGGAA TTCAAAAATA 
AATAACAGTT TGATGGTTTG TTTGAAAAAA AACTCCACCA CGTTTGTTCA ATTACAATTA 
ATATTCTTCT CTTCAGCTAT TCAATTCGAT CATGAATTAT TACTGGCACG TCTGAGCATC 
GATACAATGG CCAACAATTT AAACATGCAG AAGCTATGCC TTTATACTGA AGGATTCAGG 
ATATTCTTCG ACATACCAAG TAAGAAGGAA TTGCGGAAGG CAATTGCGGT TAAAATTTCT 
AAATTTTTCA AAACATTATT CTCCATTATA GCAGATATTC TTTTACAAGA ATTTCCGTAT 
TTTGATGAGC AAATCACCGA CATAGTTGCT TCCATTCTTG ACGGTACAAT TATCAATGAG 
TATGGTACGA AGAAACATTT CAAGGGGAGC TCACCCTCTT TATGTTCGAC AACCCGGTCA 
AGATCAGGAT CTACATCTCA AAGTTCAATG ACACCAGTTT CTCCGCTGGG ACTGGATACT 
GATATATGTC CAATGAACAC CCTGTCTTTA GTTGGTTCAA GTACTTCAAG AAATTCTGAC 
AACGTTAATT CATTAAACAG TTCACCAAAG AACTTGTCTT CTGATCCATA CTTGTCACAT 
CTTGTGGCCC CAAGAGCGCG TCATGCTTTA GGTGGGCCAT CTAGTATTAT AAGGAATAAA 
ATACCGACTA CATTGACTTC ACCTCCAGGA ACGGAAAAAT CTTCACCAGT ACAACGTCCG 
CAAACGGAAA GCATCAGTGC CACACCAATG GCCATAACAA ATTCTACTCC ATTATCGTCG 
GCAGCATTCG GAATTCGATC GCCTTTGCAG AAAATAAGAA CGAGGCGTTA TTCCGATGAA 
AGTTTAGGAA AATTCATGAA ATCAACAAAT AATTACATTC AAGAACATTT GATACCAAAA 
GATTTGAATG AAGCAACTCT TCAAGATGCT AGAAGAATAA TGATTAATAT TTTCAGTATT 
TTTAAGAGAC CGAATAGTTA CTTCATCATT CCTCACAATA TAAACTCGAA TTTACAATGG 
GTTTCGCAGG ATTTTAGAAA TATTATGAAA CCGATTTTCG TCGCCATCGT AAGTCCGGAT 
GTAGATTTAC AGAATACTGC TCAATCATTC ATGGATACCT TATTATCGAA TGTTATTACT 
TATGGTGAAT CAGATGAGAA TATCAGTATT GAAGGGTATC ATCTTCTTTG CAGTTACACT 
GTAACATTAT TTGCAATGGG CCTTTTCGAT TTGAAAATTA ATAATGAAAA GCGTCAAATT 
CTCTTGGATA TAACTGTCAA GTTTATGAAG GTTAGATCAC ATTTAGCAGG GATCGCGGAG 
GCCTCACACC ACATGGAATA CATAAGTGAT TCTGAAAAAC TCACCTTTCC GCTGATTATG 
GGGACTGTTG GTAGGGCCCT ATTTGTTTCA TTATACTCTA GTCAACAAAA AATTGAAAAG 
ACTTTAAAGA TTGCTTACAC AGAGTATCTT TCTGCAATCA ATTTTCATGA GAGGAATATT 
GATGATGCTG ATAAAACTTG GGTTCATAAT ATTGAGTTTG TAGAAGCGAT GTGTCATGAC 
AACTACACAA CTTCTGGTTC AATTGCTTTC CAAAGGAGGA CAAGAAATAA TATTTTACGA 
TTTGCTACTA TTCCTAACGC TATCTTACTT GATTCTATGA GGATGATCTA TAAGAAGTGG 
CATACTTACA CACACAGTAA AAGTTTAGAA AAACAAGAAC GGAACGACTT CAGAAATTTC 
GCGGGTATTT TAGCCTCTTT GTCGGGTATC CTATTCATCA ATAAAAAGAT ATTGCAAGAA 
ATGTATCCAT ACCTACTCGA CACCGTTTCA GAATTGAAAA AAAATATAGA CTCTTTTATC 
TCAAAACAAT GCCAATGGTT AAACTATCCG GATTTATTAA CGAGAGAAAA TTCAAGAGAT 
ATTCTAAGTG TAGAACTGCA TCCTTTGTCT TTTAACTTAC TTTTTAATAA TTTGAGGCTC 
AAGTTAAAAG AACTTGCTTG TTCAGACTTA TCAATACCAG AAAATGAAAG TTCCTATGTT 
TTATTAGAAC AAATAATCAA AATGCTGCGG ACAATCCTAG GTCGTGATGA TGACAATTAT 
GTAATGATGC TTTTTTCCAC AGAGATTGTA GATCTTATTG ATTTATTGAC AGATGAAATA 
AAAAAAATAC CAGCCTATTG TCCAAAATAT CTCAAGGCAA TTATTCAAAT GACCAAAATG 
TTCAGTGCCT TGCAGCACTC AGAGGTTAAT TTAGGTGTCA AAAATCATTT TCACGTTAAA 
AATAAATGGT TGAGGCAAAT CACTGATTGG TTTCAAGTGA GTATTGCGAG AGAGTACGAT 
TTCGAAAACT TGTCAAAACC TCTAAAAGAA ATGGATTTGG TAAAAAGAGA CATGGATATT 
CTATACATAG ATACGGCAAT CGAAGCTTCA ACCGCTATTG CGTACCTCAC GAGACATACT 
TTCTTAGAGA TTCCACCTGC CGCGTCAGAT CCCGAACTAT CTCGATCTAG GTCTGTGATA 
TTTGGGTTTT ATTTCAACAT CTTAATGAAA GGCCTTGAAA AAAGTAGTGA TCGTGACAAT 
TACCCAGTAT TCTTGAGGCA CAAAATGAGT GTCCTCAACG ACAATGTAAT ACTTTCATTA 
ACAAATCTTT CAAACACCAA TGTTGATGCG AGTTTGCAGT TCACCTTACC GATGGGCTAT 
TCCGGAAATC GAAACATTAG GAATGCATTT TTGGAGGTCT TCATTAATAT CGTTACGAAC 
TATCGGACAT ACACGGCTAA AACTGACCTT GGAAAATTAG AGGCAGCAGA CAAATTTTTG 
CGATATACGA TTGAACATCC CCAGCTATCG TCCTTTGGAG CAGCGGTTTG TCCCGCTAGC 
GATATTGATG CTTATGCTGC TGGCTTAATA AATGCATTTG AAACGAGGAA TGCCACCCAC 
ATTGTAGTGG CACAGTTGAT TAAAAATGAA ATTGAAAAAT CTTCCAGACC TACGGATATC 
CTTAGAAGAA ATAGCTGTGC TACGAGATCA TTATCTATGC TAGCCAGGTC CAAGGGTAAC 
GAATATTTGA TTCGCACTTT GCAACCATTA CTAAAAAAAA TTATCCAGAA CAGAGATTTT 
TTTGAAATTG AGAAACTAAA ACCGGAAGAT TCAGATGCTG AACGTCAAAT AGAGCTCTTC 
GTTAAATACA TGAATGAATT ATTGGAATCC ATATCCAACT CCGTATCTTA TTTTCCCCCT 
CCTTTATTTT ATATTTGCCA AAACATTTAT AAAGTTGCGT GTGAAAAATT TCCGGATCAC 
GCAATTATCG CCGCTGGGTC TTTCGTGTTT TTACGGTTTT TTTGTCCTGC TTTAGTCAGC 
CCTGATTCTG AAAATATCAT AGATATTTCT CACTTGAGCG AAAAGCGTAC CTTCATCAGC 
TTGGCTAAAG TTATCCAAAA TATTGCCAAT GGCTCAGAAA ATTTCTCCAG ATGGCCAGCT 
TTGTGTTCCC AAAAGGATTT TCTTAAGGAA TGTAGCGATA GAATTTTCAG ATTCCTAGCT 
GAACTTTGTA GAACAGATCG CACGATAGAC ATCCAAGTGA GAACAGACCC AACGCCAATT 
GCATTTGACT ATCAATTCCT TCATTCCTTT GTTTACCTTT ACGGTCTTGA GGTGAGAAGG 
AATGTGCTAA ATGAAGCAAA ACATGATGAT GGTGACATTG ATGGTGACGA TTTCTATAAG 
ACCACATTTT TACTTATTGA TGATGTTCTT GGCCAATTAG GCCAACCTAA AATGGAATTT 
TCCAATGAAA TACCAATATA CATAAGAGAA CATATGGACG ACTATCCGGA ACTGTATGAG 
TTCATGAATA GGCACGCGTT CAGAAACATT GAGACTTCAA CAGCGTACAG CCCAAGCGTT 
CACGAGTCCA CCTCAAGTGA AGGCATTCCA ATTATTACGT TAACAATGTC AAATTTCTCA 
GACAGACATG TGGACATTGA TACAGTTGCT TACAAGTTCT TGCAAATTTA TGCTCGAATC 
TGGACCACCA AACACTGTTT AATAATCGAC TGTACAGAAT TTGACGAGGG AGGGCTTGAT 
ATGAGGAAAT TTATTTCTTT GGTTATGGGA CTATTACCAG AAGTTGCACC CAAAAATTGT 
ATAGGCTGTT ACTACTTTAA CGTAAACGAG ACATTTATGG ATAATTATGG AAAATGTTTG 
GACAAAGACA ACGTATATGT TTCCTCGAAA ATTCCTCATT ATTTCATTAA TAGTAACTCT 
GATGAAGGAC TTATGAAATC TGTGGGTATA ACTGGACAAG GGTTGAAGGT TCTGCAAGAT 
ATTCGTGTCT CTCTGCATGA TATCACGCTT TATGACGAAA AAAGAAATAG ATTTACGCCG 
GTATCGTTGA AAATAGGCGA TATTTACTTT CAAGTCTTGC ATGAAACTCC TAGGCAATAT 
AAAATAAGGG ACATGGGTAC TTTATTCGAC GTAAAATTCA ATGATGTCTA CGAAATTAGC 
CGAATATTTG AAGTACATGT TTCGTCAATA ACTGGAGTGG CAGCTGAATT TACAGTAACT 
TTTCAGGACG AGAGAAGGTT GATTTTTAGT AGTCCGAAAT ACCTTGAAAT TGTGAAGATG 
TTCTATTACG CACAGATCCG GTTAGAAAGT GAATATGAAA TGGATAATAA TTCGAGTACC 
TCCTCCCCAA ATTCAAACAA CAAGGACAAA CAGCAGAAAG AGAGAACAAA ACTATTGTGC 
CACCTACTGT TAGTATCTCT TATTGGTCTG TTTGATGAGA GTAAAAAAAT GAAAAACAGT 
TCGTATAACC TAATAGCTGC CACTGAGGCG TCATTTGGTT TGAACTTTGG CTCCCATTTT 
CATCGCTCTC CCGAGGTGTA CGTCCCCGAA GATACTACAA CATTTTTAGG TGTTATTGGA 
AAGTCTCTTG CAGAGTCTAA TCCAGAACTC ACAGCCTATA TGTTTATCTA TGTTTTGGAG 
GCATTGAAGA ACAACGTAAT TCCTCACGTT TACATCCCTC ATACCATTTG CGGTTTGTCT 
TATTGGATCC CTAATTTATA CCAACATGTG TATTTGGCTG ATGATGAAGA AGGCCCCGAA 
AACATATCTC ACATTTTCCG AATTCTTATC AGGCTCTCTG TGAGAGAGAC TGACTTTAAA 
GCCGTATACA TGCAATATGT TTGGTTGCTA CTTTTAGATG ATGGCCGCTT AACTGACATT 
ATCGTTGATG AAGTTATTAA TCATGCGTTA GAAAGAGACT CCGAAAACCG CGATTGGAAG 
AAAACAATAT CGTTACTGAC TGTCCTACCC ACTACTGAGG TTGCTAATAA TATTATTCAA 
AAAATATTGG CAAAAATTAG ATCATTTTTA CCGTCATTGA AGTTAGAAGC TATGACCCAA 
AGTTGGTCTG AACTAACAAT ATTAGTTAAG ATAAGCATCC ACGTTTTTTT TGAAACTTCT 
TTGCTGGTAC AGATGTACTT ACCAGAGATC CTGTTTATCG TATCCTTATT AATTGATGTT 
GGTCCAAGGG AACTCAGATC ATCACTACAC CAGCTATTAA TGAATGTATG CCATTCCTTG 
GCTATTAACT CAGCTTTACC ACAAGATCAT AGAAATAATC TAGATGAAAT AAGTGATATA 
TTTGCACATC AAAAGGTGAA GTTTATGTTT GGGTTCAGCG AGGACAAAGG ACGAATTTTA 
CAGATTTTTA GCGCTTCTTC TTTTGCAAGC AAGTTTAATA TTCTGGATTT CTTCATCAAT 
AATATATTAT TGCTGATGGA ATATTCTTCA ACGTACGAAG CAAACGTGTG GAAGACAAGA 
TACAAGAAAT ATGTCTTGGA ATCTGTGTTT ACAAGTAATT CTTTTCTTTC GGCACGTTCA 
ATCATGATTG TTGGTATAAT GGGTAAATCT TACATAACTG AAGGGTTATG CAAGGCTATG 
TTAATTGAAA CCATGAAAGT TATCGCCGAA CCAAAGATTA CTGACGAGCA TCTTTTCTTA 
GCCATATCTC ATATTTTTAC TTATTCCAAA ATTGTTGAAG GTTTGGATCC CAACCTTGAC 
TTAATGAAGC ACTTATTTTG GTTTTCAACA CTCTTCCTTG AATCACGTCA CCCGATAATT 
TTTGAGGGTG CCCTTCTCTT TGTGTCAAAC TGTATAAGGC GCCTATACAT GGCCCAGTTT 
GAAAATGAAA GCGAAACATC ATTGATAAGT ACTTTACTTA AGGGGAGAAA GTTTGCTCAT 
ACCTTTTTAA GCAAGATAGA GAATCTTAGT GGTATTGTTT GGAATGAAGA TAATTTTACA 
CACATTCTGA TTTTCATCAT TAATAAAGGA CTATCCAATC CTTTCATTAA GAGTACGGCT 
TTTGATTTCT TGAAGATGAT GTTTAGAAAC TCCTACTTTG AGCATCAAAT CAATCAGAAA 
TCTGATCATT ATTTGTGCTA TATGTTCCTA TTGTATTTTG TTTTAAACTG CAATCAATTT 
GAGGAACTTT TAGGTGACGT TGATTTTGAA GGAGAAATGG TTAACATTGA AAACAAGAAC 
ACCATTCCTA AAATTTTGTT AGAGTGGTTG AGTTCGGATA ACGAAAATGC AAACATTACC 
CTCTATCAAG GTGCGATACT GTTCAAATGT TCAGTTACGG ATGAACCAAG TAGATTTAGG 
TTTGCGTTGA TTATTAGGCA TCTATTGACA AAGAAACCCA TTTGTGCATT GCGTTTTTAC 
AGTGTTATTC GTAACGAAAT AAGAAAAATA TCAGCATTTG AGCAAAATTC GGATTGTGTT 
CCACTTGCTT TCGATATTTT AAACTTATTA GTGACGCATT CAGAGTCTAA TTCGTTAGAA 
AAACTTCACG AAGAATCCAT TGAACGTCTA ACCAAAAGAG GTTTATCGAT TGTGACTTCT 
TCTGGTATAT TTGCGAAGAA CTCCGACATG ATGATACCTT TAGATGTAAA ACCTGAAGAT 
ATCTATGAAC GTAAGAGAAT AATGACAATG ATTTTATCAA GGATGTCATG TTCTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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IRA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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