PKH2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PKH2
Entrez Gene ID	854053
Full Name	serine/threonine protein kinase PKH2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183354.1	NP_014541.1	serine/threonine protein kinase PKH2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PKH2	NP_014541.1
Eremothecium gossypii	AGOS_AFR335C	NP_985882.1
Kluyveromyces lactis	KLLA0C12485g	XP_452755.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, Sch9 is a Cst6/Rca1 kinase that links CO2 adaptation to lipid signaling via Pkh1/2 in fungi.
Title: Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9.

PI kinase-mediated Endoplasmic Reticulum-Plasma Membrane cross-talk comprises a regulatory system that ensures cellular integrity under membrane stress conditions.
Title: Phosphoinositide kinase signaling controls ER-PM cross-talk.

Vps27 phosphorylation by Pkh1/2 kinases regulates the coordinated cascade of endosomal sorting complex required for transport complex recruitment at the endosomal membrane.
Title: Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes.

Data show a mechanism through which the conserved Pkh-Ypk kinase cascade and Cdc55-PP2A facilitate rapid, transient sphingolipid production in response to heat stress through Orm protein phosphoregulation.
Title: Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways.

Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast
Title: Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast.

The following PKH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03221	NM_001183354.1 Latest version!	Saccharomyces cerevisiae S288C serine/threonine protein kinase PKH2 (PKH2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PKH2 ( NM_001183354.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014541.1 Saccharomyces cerevisiae S288C serine/threonine protein kinase PKH2 (PKH2), partial mRNA.

Price & Availability↑

CloneID	OSi03221
Clone ID Related Accession (Same CDS sequence)	NM_001183354.1
Accession Version	NM_001183354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	serine/threonine protein kinase PKH2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTATTTTG ATAAGGATAA TTCCATGAGC CCTAGGCCGT TATTGCCAAG TGATGAGCAG 
AAGCTAAACA TTAATCTTCT AACGAAAAAG GAGAAATTCT CGCATTTAGA CCCCCATTAT 
GACGCAAAAG CCACTCCACA AAGAAGCACT TCGAATAGAA ACGTTGGCGA TTTACTTTTG 
GAAAAAAGAA CCGCTAAGCC TATGATTCAA AAGGCCTTGA CGAATACGGA TAATTTCATT 
GAAATGTACC ATAATCAGCA GAGAAAAAAT CTTGATGATG ACACTATTAA AGAAGTAATG 
ATTAATGATG AAAACGGAAA AACTGTCGCT AGTACCAACG ACGGCAGATA TGACAACGAT 
TACGATAATA ACGATATTAA TGACCAAAAA ACTTTGGATA ATATAGCGGG AAGTCCCCAC 
ATGGAAAAAA ATCGAAACAA AGTAAAGATT GAACATGACT CTTCATCTCA AAAACCAATA 
GCTAAAGAGT CATCCAAAGC CCAAAAAAAT ATAATCAAAA AGGGAATCAA GGACTTTAAA 
TTTGGTAGTG TAATAGGTGA TGGCGCGTAT TCTACTGTAA TGTTAGCGAC GTCGATTGAT 
ACCAAAAAGA GGTACGCCGC AAAAGTACTA AACAAAGAAT ATTTAATACG CCAGAAGAAA 
GTCAAATACG TCAGCATAGA AAAAACCGCC CTTCAAAAGC TCAATAATTC TCCTAGTGTT 
GTGCGATTAT TTTCCACTTT TCAGGATGAA TCAAGCCTAT ACTTTCTCTT AGAGTATGCC 
CCCAATGGGG ACTTTCTTTC TTTAATGAAA AAATACGGTT CATTAGACGA AACCTGCGCA 
CGATATTATG CTGCGCAAAT AATAGATGCC ATAGACTACT TACATTCCAA CGGTATTATT 
CATAGAGATA TAAAACCAGA AAATATTCTT TTAGATGGAG AAATGAAGAT CAAACTGACT 
GATTTTGGTA CTGCGAAGTT ACTGAATCCT ACAAATAATA GCGTTTCGAA ACCAGAATAC 
GATTTATCAA CAAGGTCGAA ATCTTTCGTT GGAACTGCAG AATACGTATC TCCAGAACTT 
TTAAATGACA GTTTTACAGA CTATCGTTGC GATATTTGGG CCTTCGGATG TATACTTTTC 
CAGATGATTG CCGGAAAACC ACCATTCAAA GCTACCAATG AATACTTGAC TTTCCAAAAG 
GTAATGAAAG TTCAGTACGC CTTTACACCA GGTTTCCCAC TTATTATCAG AGATTTGGTT 
AAGAAAATCT TAGTAAAAAA CTTAGACCGA AGATTGACGA TAAGCCAAAT TAAGGAACAT 
CATTTTTTCA AAGATTTGAA TTTTAAAGAC GGCTCTGTTT GGTCAAAAAC GCCTCCAGAG 
ATCAAACCAT ATAAAATCAA CGCCAAATCC ATGCAGGCAA TGCCAAGCGG AAGCGATAGA 
AAACTGGTGA AGAAATCAGT CAACACACTT GGCAAATCGC ATCTAGTGAC TCAAAGGTCA 
GCTTCAAGTC CCTCTGTTGA GGAAACTACT CATTCAACCC TATACAATAA CAATACTCAC 
GCTTCTACTG AAAGTGAAAT ATCAATAAAG AAGAGACCCA CTGATGAAAG AACAGCGCAG 
ATACTTGAAA ATGCAAGAAA GGGTATAAAC AATAGGAAAA ATCAACCAGG CAAGAGAACA 
CCAAGTGGTG CAGCTTCTGC TGCCCTAGCA GCTTCTGCTG CTTTAACCAA GAAAACCATG 
CAAAGCTATC CAACTTCTAG TTCGAAAAGT AGCAGGTCAA GCTCTCCTGC GACAACATCA 
AGACCAGGAA CTTATAAGCG TACTTCTTCT ACAGAAAGTA AACCATTTGC CAAATCTCCA 
CCTTTGTCAG CATCAGTTTT ATCGTCAAAA GTCCCAATGC CTCCATACAC ACCTCCAATG 
TCGCCCCCTA TGACACCATA TGATACATAT CAAATGACAC CTCCCTATAC GACAAAACAG 
CAGGATTATT CTGATACCGC AATTGCCGCA CCTAAGCCTT GTATTAGTAA GCAAAATGTT 
AAAAATAGCA CAGATTCTCC CTTGATGAAC AAGCAAGATA TTCAATGGTC CTTTTACCTG 
AAAAACATCA ACGAACATGT ACTAAGGACG GAAAAACTGG ATTTTGTTAC CACAAATTAC 
GATATCTTAG AGAAGAAAAT GCTTAAACTA AATGGTTCAT TGTTAGATCC TCAACTGTTT 
GGTAAGCCTA GACATACTTT TTTATCCCAA GTAGCTAGGA GTGGGGGAGA GGTTACAGGT 
TTTCGAAATG ATCCAACTAT GACTGCTTAT TCCAAAACAG AAGATACGTA CTATTCGAAA 
AATATTATCG ATTTGCAGCT CTTGGAAGAT GATTATCGAA TTGAAGGAGG TGACTTATCG 
GAGTTGCTTA CTAACAGAAG CGGAGAAGGG TACAAATGCA ATCAAAACAG CTCACCAATG 
AAAGACGATG ATAAATCCGA ATCTAACAAT AAAGGAAGCT CTGTTTTTTC TGGCAAGATT 
AAAAAATTAT TTCACCCTAC CTCAGCAGCT GAAACGCTCT CTTCCTCTGA TGAAAAAACC 
AAGTACTATA AACGAACCAT TGTAATGACA TCATTTGGAA GGTTTCTAGT ATTTGCCAAG 
AGGAGGCAGC CAAATCCAGT TACAAATTTA AAGTATGAAC TAGAATATGA CATAAATTTG 
CGTCAACAGG GTACCAAAAT AAAAGAACTA ATCATTCCCT TGGAAATGGG AACTAATCAT 
ATAGTTGTGA TTCAGACACC TTACAAGTCA TTTCTTTTGA GCACTGATAA AAAAACCACG 
AGCAAATTGT TTACTGTTCT CAAAAAAATT CTTAATTCGA ATACAAATAA AATAGAGAAA 
GAACTGTTGC AAAGAAACCA AAAGGTAATT GAAAGAAGAA CATCATCATC CGGAAGAGCC 
ATACCTAAAG ATCTTCCAAC TTCCAAGTCT CCTTCGCCAA AACCCAGGAC GCATAGCCAA 
TCTCCATCAA TTTCAAAGCA CAATTCGTTT TCTGAATCGA TTAATAGCGC TAAGAGCAAC 
AGATCAAGCA GAATTTTTGA AACCTTTATC AATGCCAAGG AACAAAATTC AAAAAAACAC 
GCTGCTCCAG TACCGTTAAC CAGTAAATTA GTTAACGGAT TGCCAAAAAG ACAAGTTACC 
GTGGGATTAG GTCTAAACAC AGGAACAAAT TTCAAAAACT CATCTGCAAA ATCGAAGAGG 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014541.1
CDS	1..3246
Translation	MYFDKDNSMSPRPLLPSDEQKLNINLLTKKEKFSHLDPHYDAKATPQRSTSNRNVGDLLLEKRTAKPMIQKALTNTDNFIEMYHNQQRKNLDDDTIKEVMINDENGKTVASTNDGRYDNDYDNNDINDQKTLDNIAGSPHMEKNRNKVKIEHDSSSQKPIAKESSKAQKNIIKKGIKDFKFGSVIGDGAYSTVMLATSIDTKKRYAAKVLNKEYLIRQKKVKYVSIEKTALQKLNNSPSVVRLFSTFQDESSLYFLLEYAPNGDFLSLMKKYGSLDETCARYYAAQIIDAIDYLHSNGIIHRDIKPENILLDGEMKIKLTDFGTAKLLNPTNNSVSKPEYDLSTRSKSFVGTAEYVSPELLNDSFTDYRCDIWAFGCILFQMIAGKPPFKATNEYLTFQKVMKVQYAFTPGFPLIIRDLVKKILVKNLDRRLTISQIKEHHFFKDLNFKDGSVWSKTPPEIKPYKINAKSMQAMPSGSDRKLVKKSVNTLGKSHLVTQRSASSPSVEETTHSTLYNNNTHASTESEISIKKRPTDERTAQILENARKGINNRKNQPGKRTPSGAASAALAASAALTKKTMQSYPTSSSKSSRSSSPATTSRPGTYKRTSSTESKPFAKSPPLSASVLSSKVPMPPYTPPMSPPMTPYDTYQMTPPYTTKQQDYSDTAIAAPKPCISKQNVKNSTDSPLMNKQDIQWSFYLKNINEHVLRTEKLDFVTTNYDILEKKMLKLNGSLLDPQLFGKPRHTFLSQVARSGGEVTGFRNDPTMTAYSKTEDTYYSKNIIDLQLLEDDYRIEGGDLSELLTNRSGEGYKCNQNSSPMKDDDKSESNNKGSSVFSGKIKKLFHPTSAAETLSSSDEKTKYYKRTIVMTSFGRFLVFAKRRQPNPVTNLKYELEYDINLRQQGTKIKELIIPLEMGTNHIVVIQTPYKSFLLSTDKKTTSKLFTVLKKILNSNTNKIEKELLQRNQKVIERRTSSSGRAIPKDLPTSKSPSPKPRTHSQSPSISKHNSFSESINSAKSNRSSRIFETFINAKEQNSKKHAAPVPLTSKLVNGLPKRQVTVGLGLNTGTNFKNSSAKSKRS

Target ORF information:

RefSeq Version	NM_001183354.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C serine/threonine protein kinase PKH2 (PKH2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183354.1

ORF Insert Sequence:

ATGTATTTTG ATAAGGATAA TTCCATGAGC CCTAGGCCGT TATTGCCAAG TGATGAGCAG 
AAGCTAAACA TTAATCTTCT AACGAAAAAG GAGAAATTCT CGCATTTAGA CCCCCATTAT 
GACGCAAAAG CCACTCCACA AAGAAGCACT TCGAATAGAA ACGTTGGCGA TTTACTTTTG 
GAAAAAAGAA CCGCTAAGCC TATGATTCAA AAGGCCTTGA CGAATACGGA TAATTTCATT 
GAAATGTACC ATAATCAGCA GAGAAAAAAT CTTGATGATG ACACTATTAA AGAAGTAATG 
ATTAATGATG AAAACGGAAA AACTGTCGCT AGTACCAACG ACGGCAGATA TGACAACGAT 
TACGATAATA ACGATATTAA TGACCAAAAA ACTTTGGATA ATATAGCGGG AAGTCCCCAC 
ATGGAAAAAA ATCGAAACAA AGTAAAGATT GAACATGACT CTTCATCTCA AAAACCAATA 
GCTAAAGAGT CATCCAAAGC CCAAAAAAAT ATAATCAAAA AGGGAATCAA GGACTTTAAA 
TTTGGTAGTG TAATAGGTGA TGGCGCGTAT TCTACTGTAA TGTTAGCGAC GTCGATTGAT 
ACCAAAAAGA GGTACGCCGC AAAAGTACTA AACAAAGAAT ATTTAATACG CCAGAAGAAA 
GTCAAATACG TCAGCATAGA AAAAACCGCC CTTCAAAAGC TCAATAATTC TCCTAGTGTT 
GTGCGATTAT TTTCCACTTT TCAGGATGAA TCAAGCCTAT ACTTTCTCTT AGAGTATGCC 
CCCAATGGGG ACTTTCTTTC TTTAATGAAA AAATACGGTT CATTAGACGA AACCTGCGCA 
CGATATTATG CTGCGCAAAT AATAGATGCC ATAGACTACT TACATTCCAA CGGTATTATT 
CATAGAGATA TAAAACCAGA AAATATTCTT TTAGATGGAG AAATGAAGAT CAAACTGACT 
GATTTTGGTA CTGCGAAGTT ACTGAATCCT ACAAATAATA GCGTTTCGAA ACCAGAATAC 
GATTTATCAA CAAGGTCGAA ATCTTTCGTT GGAACTGCAG AATACGTATC TCCAGAACTT 
TTAAATGACA GTTTTACAGA CTATCGTTGC GATATTTGGG CCTTCGGATG TATACTTTTC 
CAGATGATTG CCGGAAAACC ACCATTCAAA GCTACCAATG AATACTTGAC TTTCCAAAAG 
GTAATGAAAG TTCAGTACGC CTTTACACCA GGTTTCCCAC TTATTATCAG AGATTTGGTT 
AAGAAAATCT TAGTAAAAAA CTTAGACCGA AGATTGACGA TAAGCCAAAT TAAGGAACAT 
CATTTTTTCA AAGATTTGAA TTTTAAAGAC GGCTCTGTTT GGTCAAAAAC GCCTCCAGAG 
ATCAAACCAT ATAAAATCAA CGCCAAATCC ATGCAGGCAA TGCCAAGCGG AAGCGATAGA 
AAACTGGTGA AGAAATCAGT CAACACACTT GGCAAATCGC ATCTAGTGAC TCAAAGGTCA 
GCTTCAAGTC CCTCTGTTGA GGAAACTACT CATTCAACCC TATACAATAA CAATACTCAC 
GCTTCTACTG AAAGTGAAAT ATCAATAAAG AAGAGACCCA CTGATGAAAG AACAGCGCAG 
ATACTTGAAA ATGCAAGAAA GGGTATAAAC AATAGGAAAA ATCAACCAGG CAAGAGAACA 
CCAAGTGGTG CAGCTTCTGC TGCCCTAGCA GCTTCTGCTG CTTTAACCAA GAAAACCATG 
CAAAGCTATC CAACTTCTAG TTCGAAAAGT AGCAGGTCAA GCTCTCCTGC GACAACATCA 
AGACCAGGAA CTTATAAGCG TACTTCTTCT ACAGAAAGTA AACCATTTGC CAAATCTCCA 
CCTTTGTCAG CATCAGTTTT ATCGTCAAAA GTCCCAATGC CTCCATACAC ACCTCCAATG 
TCGCCCCCTA TGACACCATA TGATACATAT CAAATGACAC CTCCCTATAC GACAAAACAG 
CAGGATTATT CTGATACCGC AATTGCCGCA CCTAAGCCTT GTATTAGTAA GCAAAATGTT 
AAAAATAGCA CAGATTCTCC CTTGATGAAC AAGCAAGATA TTCAATGGTC CTTTTACCTG 
AAAAACATCA ACGAACATGT ACTAAGGACG GAAAAACTGG ATTTTGTTAC CACAAATTAC 
GATATCTTAG AGAAGAAAAT GCTTAAACTA AATGGTTCAT TGTTAGATCC TCAACTGTTT 
GGTAAGCCTA GACATACTTT TTTATCCCAA GTAGCTAGGA GTGGGGGAGA GGTTACAGGT 
TTTCGAAATG ATCCAACTAT GACTGCTTAT TCCAAAACAG AAGATACGTA CTATTCGAAA 
AATATTATCG ATTTGCAGCT CTTGGAAGAT GATTATCGAA TTGAAGGAGG TGACTTATCG 
GAGTTGCTTA CTAACAGAAG CGGAGAAGGG TACAAATGCA ATCAAAACAG CTCACCAATG 
AAAGACGATG ATAAATCCGA ATCTAACAAT AAAGGAAGCT CTGTTTTTTC TGGCAAGATT 
AAAAAATTAT TTCACCCTAC CTCAGCAGCT GAAACGCTCT CTTCCTCTGA TGAAAAAACC 
AAGTACTATA AACGAACCAT TGTAATGACA TCATTTGGAA GGTTTCTAGT ATTTGCCAAG 
AGGAGGCAGC CAAATCCAGT TACAAATTTA AAGTATGAAC TAGAATATGA CATAAATTTG 
CGTCAACAGG GTACCAAAAT AAAAGAACTA ATCATTCCCT TGGAAATGGG AACTAATCAT 
ATAGTTGTGA TTCAGACACC TTACAAGTCA TTTCTTTTGA GCACTGATAA AAAAACCACG 
AGCAAATTGT TTACTGTTCT CAAAAAAATT CTTAATTCGA ATACAAATAA AATAGAGAAA 
GAACTGTTGC AAAGAAACCA AAAGGTAATT GAAAGAAGAA CATCATCATC CGGAAGAGCC 
ATACCTAAAG ATCTTCCAAC TTCCAAGTCT CCTTCGCCAA AACCCAGGAC GCATAGCCAA 
TCTCCATCAA TTTCAAAGCA CAATTCGTTT TCTGAATCGA TTAATAGCGC TAAGAGCAAC 
AGATCAAGCA GAATTTTTGA AACCTTTATC AATGCCAAGG AACAAAATTC AAAAAAACAC 
GCTGCTCCAG TACCGTTAAC CAGTAAATTA GTTAACGGAT TGCCAAAAAG ACAAGTTACC 
GTGGGATTAG GTCTAAACAC AGGAACAAAT TTCAAAAACT CATCTGCAAA ATCGAAGAGG 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PKH2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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