SIS2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SIS2
Entrez Gene ID	853946
Full Name	phosphopantothenoylcysteine decarboxylase complex subunit SIS2
Synonyms	HAL3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179862.1	NP_012998.1	phosphopantothenoylcysteine decarboxylase complex subunit SIS2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Hal3 N-terminal domain is not functional by itself, although in vitro experiments indicate that when this domain is combined with the core it has a relevant function in Hal3's heteromeric phosphopantothenoylcysteine decarboxylase activity.
Title: Functional mapping of the disparate activities of the yeast moonlighting protein Hal3.

The aim of this work was to study mechanisms which relate the activity of Ppz1p/Hal3p complex to nonsense suppression efficiency.
Title: [The protein complex Ppz1p/Hal3p and nonsense suppression efficiency in the yeast Saccharomyces cerevisiae].

Results demonstrate the participation of the Hal3-Ppz1 protein complex in regulation of read-through efficiency and manifestation of non-Mendelian anti-suppressor determinant [ISP(+)].
Title: The HAL3-PPZ1 dependent regulation of nonsense suppression efficiency in yeast and its influence on manifestation of the yeast prion-like determinant [ISP(+)].

the Ppz1-Hal3 interaction is a sensor of intracellular pH that modulates H+ and K+ homeostasis through the regulation of Trk1p activity
Title: pH-Responsive, posttranslational regulation of the Trk1 potassium transporter by the type 1-related Ppz1 phosphatase.

The following SIS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03125	NM_001179862.1 Latest version!	Saccharomyces cerevisiae S288C phosphopantothenoylcysteine decarboxylase complex subunit SIS2 (SIS2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIS2 ( NM_001179862.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012998.1 Saccharomyces cerevisiae S288C phosphopantothenoylcysteine decarboxylase complex subunit SIS2 (SIS2), partial mRNA.

Price & Availability↑

CloneID	OSi03125
Clone ID Related Accession (Same CDS sequence)	NM_001179862.1
Accession Version	NM_001179862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphopantothenoylcysteine decarboxylase complex subunit SIS2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGCCG TCGCCTCTAC TAGCGGGAAG CAAGATGCTG ACCATAACCA GTCTATAGAG 
TGTCCAAGGT TTTCGCGTGG ACAGAAAGAG ATTTTGTTGG ACCATGAGGA TGCGAAGGGG 
AAGGATTCAA TAATCAACTC CCCCGTGTCA GGGAGGCAAT CAATAAGCCC TACTTTGTCG 
AATGCTACTA CCACGACTAC GAAATCTATT ATGAATGCCA CCGGCACCTC CGGCGCAGTA 
GTAAGTAATA CTCCGGAGCC TGGTTTGAAG AGAGTCCCCG CCGTCACTTT CAGTGATCTA 
AAGCAACAGC AAAAGCAGGA TAGTCTGACT CAGCTGAAGA ACGACTCAGA AAGGACCAAG 
TCTCCAAATA GTAATCCTGC TCCTGTCTCA AATTCCATTC CTGGCAATCA TGCAGTTATA 
CCGAACCACA CCAACACTTC AAGAACAACG CAATTATCTG GGTCTCCACT CGTCAACGAA 
ATGAAAGATT ATGATCCTAA GAAAAAAGAC AGTGCTCTTA AAATAGTTGA TACCATGAAG 
CCTGACAAAA TCATGGCCAC TTCAACGCCA ATAAGTAGAG AAAATAATAA GGTAACCGCC 
AAAGCCCCCA CCAGTATTAC ACTTCGAAAG GAAGATGCAC AAGATCAAGC TAACAATGTT 
TCAGGCCAAA TTAATGTGCG TTCTACCCCG GAAGAAACGC CAGTGAAGCA GTCCGTCATT 
CCTTCCATCA TCCCCAAAAG AGAGAACTCC AAGAATTTGG ATCCCAGACT ACCTCAAGAT 
GATGGAAAAT TGCACGTGTT GTTTGGCGCT ACAGGTTCGT TATCGGTATT TAAGATCAAG 
CCAATGATTA AAAAACTAGA AGAAATATAT GGACGTGATA GAATAAGCAT TCAAGTTATC 
CTCACTCAAT CAGCAACACA GTTTTTCGAA CAAAGATATA CCAAGAAAAT TATCAAATCT 
TCAGAAAAAC TAAATAAAAT GTCGCAGTAC GAATCCACTC CGGCAACTCC AGTGACACCA 
ACACCAGGAC AATGCAACAT GGCTCAAGTA GTCGAGTTAC CCCCACATAT TCAACTATGG 
ACCGACCAAG ATGAATGGGA CGCATGGAAA CAACGAACTG ATCCTGTACT TCATATAGAA 
CTACGTCGCT GGGCGGATAT ACTGGTAGTA GCTCCATTAA CTGCAAACAC ATTATCAAAA 
ATTGCTTTGG GCCTGTGCGA CAATCTTCTG ACAAGTGTCA TTAGAGCTTG GAATCCGAGT 
TATCCCATTC TTTTGGCACC TTCTATGGTG AGTAGCACTT TTAATTCTAT GATGACAAAG 
AAGCAACTAC AAACGATAAA GGAGGAAATG TCCTGGGTCA CTGTTTTCAA GCCGTCTGAG 
AAAGTTATGG ATATAAATGG TGATATTGGT CTCGGGGGTA TGATGGACTG GAATGAGATT 
GTTAACAAAA TCGTTATGAA ATTGGGTGGG TACCCAAAAA ATAACGAGGA AGAGGACGAT 
GATGAGGATG AGGAAGAAGA CGACGACGAA GAAGAAGATA CAGAAGATAA GAACGAAAAC 
AACAACGATG ATGATGACGA TGACGACGAT GACGACGACG ACGACGACGA CGACGACGAC 
GACGACGACG ATGATGACGA GGACGAGGAC GAAGCAGAAA CCCCAGGTAT AATAGATAAG 
CATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012998.1
CDS	1..1689
Translation	MTAVASTSGKQDADHNQSIECPRFSRGQKEILLDHEDAKGKDSIINSPVSGRQSISPTLSNATTTTTKSIMNATGTSGAVVSNTPEPGLKRVPAVTFSDLKQQQKQDSLTQLKNDSERTKSPNSNPAPVSNSIPGNHAVIPNHTNTSRTTQLSGSPLVNEMKDYDPKKKDSALKIVDTMKPDKIMATSTPISRENNKVTAKAPTSITLRKEDAQDQANNVSGQINVRSTPEETPVKQSVIPSIIPKRENSKNLDPRLPQDDGKLHVLFGATGSLSVFKIKPMIKKLEEIYGRDRISIQVILTQSATQFFEQRYTKKIIKSSEKLNKMSQYESTPATPVTPTPGQCNMAQVVELPPHIQLWTDQDEWDAWKQRTDPVLHIELRRWADILVVAPLTANTLSKIALGLCDNLLTSVIRAWNPSYPILLAPSMVSSTFNSMMTKKQLQTIKEEMSWVTVFKPSEKVMDINGDIGLGGMMDWNEIVNKIVMKLGGYPKNNEEEDDDEDEEEDDDEEEDTEDKNENNNDDDDDDDDDDDDDDDDDDDDDDDDEDEDEAETPGIIDKHQ

Target ORF information:

RefSeq Version	NM_001179862.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphopantothenoylcysteine decarboxylase complex subunit SIS2 (SIS2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179862.1

ORF Insert Sequence:

ATGACTGCCG TCGCCTCTAC TAGCGGGAAG CAAGATGCTG ACCATAACCA GTCTATAGAG 
TGTCCAAGGT TTTCGCGTGG ACAGAAAGAG ATTTTGTTGG ACCATGAGGA TGCGAAGGGG 
AAGGATTCAA TAATCAACTC CCCCGTGTCA GGGAGGCAAT CAATAAGCCC TACTTTGTCG 
AATGCTACTA CCACGACTAC GAAATCTATT ATGAATGCCA CCGGCACCTC CGGCGCAGTA 
GTAAGTAATA CTCCGGAGCC TGGTTTGAAG AGAGTCCCCG CCGTCACTTT CAGTGATCTA 
AAGCAACAGC AAAAGCAGGA TAGTCTGACT CAGCTGAAGA ACGACTCAGA AAGGACCAAG 
TCTCCAAATA GTAATCCTGC TCCTGTCTCA AATTCCATTC CTGGCAATCA TGCAGTTATA 
CCGAACCACA CCAACACTTC AAGAACAACG CAATTATCTG GGTCTCCACT CGTCAACGAA 
ATGAAAGATT ATGATCCTAA GAAAAAAGAC AGTGCTCTTA AAATAGTTGA TACCATGAAG 
CCTGACAAAA TCATGGCCAC TTCAACGCCA ATAAGTAGAG AAAATAATAA GGTAACCGCC 
AAAGCCCCCA CCAGTATTAC ACTTCGAAAG GAAGATGCAC AAGATCAAGC TAACAATGTT 
TCAGGCCAAA TTAATGTGCG TTCTACCCCG GAAGAAACGC CAGTGAAGCA GTCCGTCATT 
CCTTCCATCA TCCCCAAAAG AGAGAACTCC AAGAATTTGG ATCCCAGACT ACCTCAAGAT 
GATGGAAAAT TGCACGTGTT GTTTGGCGCT ACAGGTTCGT TATCGGTATT TAAGATCAAG 
CCAATGATTA AAAAACTAGA AGAAATATAT GGACGTGATA GAATAAGCAT TCAAGTTATC 
CTCACTCAAT CAGCAACACA GTTTTTCGAA CAAAGATATA CCAAGAAAAT TATCAAATCT 
TCAGAAAAAC TAAATAAAAT GTCGCAGTAC GAATCCACTC CGGCAACTCC AGTGACACCA 
ACACCAGGAC AATGCAACAT GGCTCAAGTA GTCGAGTTAC CCCCACATAT TCAACTATGG 
ACCGACCAAG ATGAATGGGA CGCATGGAAA CAACGAACTG ATCCTGTACT TCATATAGAA 
CTACGTCGCT GGGCGGATAT ACTGGTAGTA GCTCCATTAA CTGCAAACAC ATTATCAAAA 
ATTGCTTTGG GCCTGTGCGA CAATCTTCTG ACAAGTGTCA TTAGAGCTTG GAATCCGAGT 
TATCCCATTC TTTTGGCACC TTCTATGGTG AGTAGCACTT TTAATTCTAT GATGACAAAG 
AAGCAACTAC AAACGATAAA GGAGGAAATG TCCTGGGTCA CTGTTTTCAA GCCGTCTGAG 
AAAGTTATGG ATATAAATGG TGATATTGGT CTCGGGGGTA TGATGGACTG GAATGAGATT 
GTTAACAAAA TCGTTATGAA ATTGGGTGGG TACCCAAAAA ATAACGAGGA AGAGGACGAT 
GATGAGGATG AGGAAGAAGA CGACGACGAA GAAGAAGATA CAGAAGATAA GAACGAAAAC 
AACAACGATG ATGATGACGA TGACGACGAT GACGACGACG ACGACGACGA CGACGACGAC 
GACGACGACG ATGATGACGA GGACGAGGAC GAAGCAGAAA CCCCAGGTAT AATAGATAAG 
CATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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SIS2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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