SPO14 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SPO14
Entrez Gene ID	853902
Full Name	phospholipase D
Synonyms	PLD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179821.3	NP_012956.3	phospholipase D

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SPO14	NP_012956.3
Kluyveromyces lactis	KLLA0F21274g	XP_456035.1
Eremothecium gossypii	AGOS_AFR071W	NP_985618.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLD-generated phosphatidic acid and phosphatidylinositol-4,5-bisphosphate collaborate to promote SNARE-mediated fusion for prospore membrane formation.
Title: Phosphatidylinositol-4,5-bisphosphate and phospholipase D-generated phosphatidic acid specify SNARE-mediated vesicle fusion for prospore membrane formation.

These findings highlight the important interactions among PLD1, Cdc42p and Ste20p during polarized cell growth in S. cerevisiae.
Title: An upstream regulator and downstream target of phospholipase D1 activity during pheromone response in Saccharomyces cerevisiae.

Taken together, these results indicate that GCS1 is essential for sporulation and suggest that GCS1 positively regulates SPO14.
Title: The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p.

During prospore membrane growth both Spo14 and Sma1 localize to the membrane, and localization of Spo14 is independent of Sma1.
Title: Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis.

The following SPO14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPO14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03088	NM_001179821.3 Latest version!	Saccharomyces cerevisiae S288C phospholipase D (SPO14), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPO14 ( NM_001179821.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012956.3 Saccharomyces cerevisiae S288C phospholipase D (SPO14), partial mRNA.

Price & Availability↑

CloneID	OSi03088
Clone ID Related Accession (Same CDS sequence)	NM_001179821.3
Accession Version	NM_001179821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phospholipase D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179821.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTAACG TGTCAACAGC GTCTGGAACT CACTTCGCTC CACCTCAGGC AGATAGATCG 
GTGACTGAAG AAGTAGATAG AGTAAATTCG AGACCAGATG AGTTAGAAAA CCAAGAAGTA 
TTGCGGCAAT TACCTGAGAA TGGCAATCTG ACTAGCAGTT TACAAAGAGA AAAACGAAGA 
ACTCCCAACG GCAAGGAGGC GGAGAGGAAG CATGCATTGC CCAAATCCTT TGTAGATAGA 
AACCTGAGTG ATGTGTCACC GAACCACTCA TTAGACCATA TTATGCACTC TAATGAACAT 
GATCCTCGTC GAGGTTCTGA TGAGGAAAAT ATGCACCGGC TCTACAACAA TCTCCATAGC 
AGTAACAATA ATGTTCATTC TAAGCGGAAT TCTAAAAGAG AGGAAGAAAG AGCCCCTCAA 
AGGCGTTCTT CTTCTGTAGC TTACACCCAA CAGCAGTTCA ATGGCTGGAA GAAAGAGTTT 
GGCCACGCTT TTAAGAAAAT CTCTGCTATT GGTAGACTGA AATCCAGCGT AAATTCTCCT 
ACACCGGCTG GAAGCGGCCA TCGTCATAAC CAGCACCAGC ATCAACAAGT AAACGAAGAA 
GATTTGTACA CACAAAGACT AGCCTCTGAT TTATTAGATT CGTTATTGGC AGGATGCCCG 
GCCTCATTGT TCGCAAGCAC GCAATTTCTG AGAGACGAAC ATGGGAAGAG AAGGGCTCCT 
CTGCTATTAG CTAAACTAGA TGTAAGAGTG TCTCCCTTGA AAAATGACAA CAACATTTTG 
GATATTACCA ATAGCAACCA TAACCATCGT GGTAACAACA ACAACAATAC TGGCGAGAAT 
TCTGATAGGA GACCCTCTAT TCCAAGGAGC TCCAGCATTA TAAGCATTTC TTCGAATGTG 
GCGGAATTTA TGTATAGCAG GAACGAAAAC TCTTTATTTA GAATACACTT GGAATATGGG 
ATTGATGAAG ACAGGCTCAA ATGGTCTATT ATAAGAAGTT ATAAAGATAT CAAATCACTA 
CATCACAAAT TGAAAATCGT CGCGTTCCAA CAACTCACTA TAAGCAAGCT TTATAGTGAT 
AACAACAGAT ATCATTCGTT ACAACTTCCG CATTTCCCCC ATTATAAAGA AATGGTTAAA 
GAAAGAAATG TAATGGAGAA AAAAGCAGAA AATAAACCAT CCTCTGCAGC CTCAGCTCCT 
CATACATCGG AAAATAACAA TAACGATAAT GGCAGCAACA TTACATCGTT AGAAACTTTA 
TCATCTTCTG AAATATCTGA GTTTAATATA GATAACGTCA AAATGAAACA TTTACAAGAC 
TTAATTGACG AACCTGACGA TTTCTCCCAA CCGATACATT TGAGGCTAGA AAGGTATTTG 
AGACTACTGA ACATTGCATT ATGTTTGAGA CCTCATGCAA ATAGATTGTT TGAATTTTAC 
GAATTATCTC CCTTAGGCAA TTTATTAAGT CGTGAAAGTG GGTTCCAGGG CAAACAGGGG 
TATTTAGTAA TTAGATCTAC TGCCAAGGCC CAAGGTTGGA GAGTTTCTCA TTTTGGTAAA 
CATGCGTTTA AAGACATGAT CGACAGACAT ACTACCAAAT GGTTCCTGGT AAGAAATTCT 
TACCTAACTT ACGTGTCGGA TCTTTCTTCG ACAACTCCTT TAGACGTATT CTTAATAGAT 
TGGAAATTTA AGGTTAGATT TTCTGGGAAC AAGAATAACA TACTTGATAA CGAAAACGAG 
ATCAACTGGA TCATTCATGA TCCCAATTTA GAAATAAATG ACGAACTAGA AGAGTTCGGT 
ATTGAAAATG ATGCCAATAA TATCCTAGAC AAGAATGGAA AATCGAAAAC ACACCAAAAG 
AAGTCCAATA TATCTTCAAA ACTATTATTA TTGACCCTAG AAAACAGTGA AAGGAAACTG 
AAAATTATTT GCAAATCAGA GAGTTCGTTA AAGCAATGGA TGAGTTCAAT TATCAAAATG 
TCTACCTCAA CACCTTGGTC GAAACCAAAT AGATTTGGTA GTTTCGCACC TGTAAGAACA 
AATTCGTTTT GCAAATTTTT GGTAGATGGG CGTGATTATT TTTGGTCGTT AAGCGAGGCC 
CTCTTAATGG CAAAGGATGT CATATATATC CATGATTGGT GGCTATCCCC TGAATTATAC 
TTGCGACGTC CGGTGAAAGG CAACCAGGGA TTTAGAATTG ATCGTATGCT GAAAAGTTGT 
GCAGAAAAAG GTATCAAAAT TTTTATTGTG ATTTATAGGA ATGTGGGGAA TATTGTTGGG 
ACAGATAGTC TTTGGACCAA GCATTCTATG CTAAATTTAC ATCCAAATAT ACATATCATA 
AGGTCTCCCA ATCAATGGCT ACAAAATACA TATTTTTGGG CACACCACGA GAAGTTTGTC 
GTTATTGATG AAACATTTGC ATTTATTGGT GGTACAGATT TATGTTATGG TAGATATGAT 
ACTTTTGAAC ACGTTCTACG AGATGATGCA GAATCTTTAT TAGATCAAAA TTTTCCTGGT 
AAGGATTATT CAAATGCCAG AATTGCTGAT TTTCATGATT TAGATAAACC ATTCGAATCA 
ATGTATGATA GAAAAGTCAT TCCGAGGATG CCATGGCATG ATGTGCAAAT GATGACTTTG 
GGTGAGCCAG CAAGAGACTT AGCCCGACAT TTTGTTCAAA GATGGAATTA CTTATTGAGA 
GCTAAGAGAC CTAGTAGGTT AACTCCATTA CTAACCCCGC CTTCAGATTT AACTGCTGAA 
GAATTGAAGA GTTTGCCGAT GTTCGAAATT CTACGCGAAA AATCGACTTG TGAAACTCAA 
ATACTTAGAA GTGCTGGGAA CTGGTCTCTT GGGCTAAAGG AAACGGAATG CTCAATTCAA 
AATGCTTACC TGAAGTTAAT TGAACAAAGT GAACACTTTA TTTATATTGA GAATCAATTC 
TTCATTACAT CAACTGTCTG GAACGGGACT TGCGTTTTGA ATAAAATTGG TGATGCTCTC 
GTTGATAGGA TAGTCAAAGC TAATCAGGAG AAAAAACCTT GGAAAGCCTT TATTCTTATC 
CCACTAATGC CAGGATTCGA TTCTCCAGTA GATACTGCAG AAGCCTCAAG TCTTCGTTTA 
ATTATGCAAT TCCAATATCA ATCAATTTCA AGAGGTGAAC ATTCTACATT TAGTAAACTG 
AAGAAACTAA ATATTGACCC AGCTCAATAT ATTCAGTTCT TTTCACTAAG AAAATGGTCG 
ACTTTTGCGC CTAATGAAAG ATTAATCACC GAGCAGCTCT ACGTTCATGC TAAAATTCTG 
ATTGCTGATG ACAGAAGATG TATTATAGGG TCTGCTAATA TTAACGAAAG ATCACAACTG 
GGTAATAGAG ATAGTGAGGT GGCAATTCTT ATTAGAGACA CCGACTTGAT AAAGACCAAA 
ATGAATGGTG ACGATTACTA CGCGGGAAAA TTTCCTTGGG AATTAAGGCA GCGATTAATG 
AGGGAACATT TAGGTTGTGA TGTAGATCTT GTTGAGTTTG TTGAAAAGAA ATTTGAAAGG 
TTTGAAAAGT TTGCTGCAAA GAATTATGAA AAACTTCATA CCCTAAGTAA GGAGGGAGAT 
AGTGGTAACA ATTGGAGCGA TCGAGAAATG ATCGATTCCG CTATGATTGA GCTAGGTTAT 
CGTGAAATAT TTGGTTGTAA ATTTAGCCCT CAATGGAAGA GTGGTCATGG AAACAGTGTT 
GATGATGGAA GCACTCAGTG TGGCATAAAT GAAAAAGAAG TGGGAAGAGA AGATGAAAAC 
GTTTATGAAA AATTCTTCAA TTCTGTGGAC TACGGTAAAT CATCACGTAA GAGAACGCCT 
CTGCCCAAAC ATAATTTTGC ATCTTTAGGG CTAACATTCA ACCACAGGGC AGGAATTGAA 
AATGTCGGTA TACGAGACCA CAAAGTCTTA AGTACTGATC CAAGATTAAG AAAGAATGAT 
GAACATAAAA AAGAGGTTGA TGGCTACGGT CCCGATTGTT GGAAAAAGGA ATCCAACAAA 
AAATTCAAGG CCGATGCAAC TGAACAGCTA AAGGAGTGGG CATTGAACTC ATTGGCCTCG 
AAAGTTCTTG ACGATAAAGA AATGATAAAA TCAGAAATTC CAGAAGGATT TTCAAATTAT 
TTGCCCAATG AGAAAGATCT AGAAATGTAT CTCACGGATA AAACTGTTAC CAATAGAAAC 
AAATGGAGTA TGCTCAAAAG GATATGCTAC TTGCAATATT TATCTCATAA ACTCGACGAA 
AGAAAAACAC AGAGATTGAA GAAAATCAAA GATATGAGAA GACATTTAAG TTCAAGCACA 
GAAAGTACCA GAAATGGGTC TAATAGTCTG CCATTAAACG AAAAGAGCAA TGAAGGAGAA 
AGCACTAATG TCGACCAAGA TATCGAAGGC GATGAATATC ATAGATTGCA CGAAGATATT 
TTGAAAAATC AAGAACTAGA TGATGGTTCA CTGGACGATC TACTTTCTCA GATAATTCCT 
AAGATCACTA ACTTTAACAG TGGTGAAATT GATGACGCAA AAAAAGAAGA GTTATTGAAA 
CTCAACTTCA TTGACCCGTA TTCCTTCGAG GATCCTCTTA TTTCTTCGTT TTCTGAAGGG 
CTATGGTTCA CTATAGCCTT GAGAAATACA TTACTCTATA AATTAGTGTT TCATTGTCAA 
CCCGATAATG CTGTACAAAA TTGGAAAGAA TATGGAGAAT TTACTGAATT AGAACAAGAA 
TTTCAAATAA ACCAGGAAAA GTTGATTGAC TTAGAGGCTG AAAATATTAA TTCTACTACT 
ACAAATGTTG TAGATAAGGA TAGGGAAAAG GAAAAAATGA GAAAAGCCGC TGAATTGAGA 
ATGAAGTTAT CCGGTAGTTT GCTTTATGGA TTCAATCAAA AGGTTTTTGA CAAACATACG 
GCGCAGAGAA TCTTGGAAAG GATCCACGGT CATTTGGTCA TTTTCCCAAC CGAATGGTTA 
GCTAAAGAAG TTGAGAGTAG AAATTGGATT TTTAACTCGG ATAGACTTTC TCCAATGGAA 
ATCTACAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012956.3
CDS	1..5052
Translation	MSNVSTASGTHFAPPQADRSVTEEVDRVNSRPDELENQEVLRQLPENGNLTSSLQREKRRTPNGKEAERKHALPKSFVDRNLSDVSPNHSLDHIMHSNEHDPRRGSDEENMHRLYNNLHSSNNNVHSKRNSKREEERAPQRRSSSVAYTQQQFNGWKKEFGHAFKKISAIGRLKSSVNSPTPAGSGHRHNQHQHQQVNEEDLYTQRLASDLLDSLLAGCPASLFASTQFLRDEHGKRRAPLLLAKLDVRVSPLKNDNNILDITNSNHNHRGNNNNNTGENSDRRPSIPRSSSIISISSNVAEFMYSRNENSLFRIHLEYGIDEDRLKWSIIRSYKDIKSLHHKLKIVAFQQLTISKLYSDNNRYHSLQLPHFPHYKEMVKERNVMEKKAENKPSSAASAPHTSENNNNDNGSNITSLETLSSSEISEFNIDNVKMKHLQDLIDEPDDFSQPIHLRLERYLRLLNIALCLRPHANRLFEFYELSPLGNLLSRESGFQGKQGYLVIRSTAKAQGWRVSHFGKHAFKDMIDRHTTKWFLVRNSYLTYVSDLSSTTPLDVFLIDWKFKVRFSGNKNNILDNENEINWIIHDPNLEINDELEEFGIENDANNILDKNGKSKTHQKKSNISSKLLLLTLENSERKLKIICKSESSLKQWMSSIIKMSTSTPWSKPNRFGSFAPVRTNSFCKFLVDGRDYFWSLSEALLMAKDVIYIHDWWLSPELYLRRPVKGNQGFRIDRMLKSCAEKGIKIFIVIYRNVGNIVGTDSLWTKHSMLNLHPNIHIIRSPNQWLQNTYFWAHHEKFVVIDETFAFIGGTDLCYGRYDTFEHVLRDDAESLLDQNFPGKDYSNARIADFHDLDKPFESMYDRKVIPRMPWHDVQMMTLGEPARDLARHFVQRWNYLLRAKRPSRLTPLLTPPSDLTAEELKSLPMFEILREKSTCETQILRSAGNWSLGLKETECSIQNAYLKLIEQSEHFIYIENQFFITSTVWNGTCVLNKIGDALVDRIVKANQEKKPWKAFILIPLMPGFDSPVDTAEASSLRLIMQFQYQSISRGEHSTFSKLKKLNIDPAQYIQFFSLRKWSTFAPNERLITEQLYVHAKILIADDRRCIIGSANINERSQLGNRDSEVAILIRDTDLIKTKMNGDDYYAGKFPWELRQRLMREHLGCDVDLVEFVEKKFERFEKFAAKNYEKLHTLSKEGDSGNNWSDREMIDSAMIELGYREIFGCKFSPQWKSGHGNSVDDGSTQCGINEKEVGREDENVYEKFFNSVDYGKSSRKRTPLPKHNFASLGLTFNHRAGIENVGIRDHKVLSTDPRLRKNDEHKKEVDGYGPDCWKKESNKKFKADATEQLKEWALNSLASKVLDDKEMIKSEIPEGFSNYLPNEKDLEMYLTDKTVTNRNKWSMLKRICYLQYLSHKLDERKTQRLKKIKDMRRHLSSSTESTRNGSNSLPLNEKSNEGESTNVDQDIEGDEYHRLHEDILKNQELDDGSLDDLLSQIIPKITNFNSGEIDDAKKEELLKLNFIDPYSFEDPLISSFSEGLWFTIALRNTLLYKLVFHCQPDNAVQNWKEYGEFTELEQEFQINQEKLIDLEAENINSTTTNVVDKDREKEKMRKAAELRMKLSGSLLYGFNQKVFDKHTAQRILERIHGHLVIFPTEWLAKEVESRNWIFNSDRLSPMEIYN

Target ORF information:

RefSeq Version	NM_001179821.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phospholipase D (SPO14), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179821.3

ORF Insert Sequence:

ATGTCTAACG TGTCAACAGC GTCTGGAACT CACTTCGCTC CACCTCAGGC AGATAGATCG 
GTGACTGAAG AAGTAGATAG AGTAAATTCG AGACCAGATG AGTTAGAAAA CCAAGAAGTA 
TTGCGGCAAT TACCTGAGAA TGGCAATCTG ACTAGCAGTT TACAAAGAGA AAAACGAAGA 
ACTCCCAACG GCAAGGAGGC GGAGAGGAAG CATGCATTGC CCAAATCCTT TGTAGATAGA 
AACCTGAGTG ATGTGTCACC GAACCACTCA TTAGACCATA TTATGCACTC TAATGAACAT 
GATCCTCGTC GAGGTTCTGA TGAGGAAAAT ATGCACCGGC TCTACAACAA TCTCCATAGC 
AGTAACAATA ATGTTCATTC TAAGCGGAAT TCTAAAAGAG AGGAAGAAAG AGCCCCTCAA 
AGGCGTTCTT CTTCTGTAGC TTACACCCAA CAGCAGTTCA ATGGCTGGAA GAAAGAGTTT 
GGCCACGCTT TTAAGAAAAT CTCTGCTATT GGTAGACTGA AATCCAGCGT AAATTCTCCT 
ACACCGGCTG GAAGCGGCCA TCGTCATAAC CAGCACCAGC ATCAACAAGT AAACGAAGAA 
GATTTGTACA CACAAAGACT AGCCTCTGAT TTATTAGATT CGTTATTGGC AGGATGCCCG 
GCCTCATTGT TCGCAAGCAC GCAATTTCTG AGAGACGAAC ATGGGAAGAG AAGGGCTCCT 
CTGCTATTAG CTAAACTAGA TGTAAGAGTG TCTCCCTTGA AAAATGACAA CAACATTTTG 
GATATTACCA ATAGCAACCA TAACCATCGT GGTAACAACA ACAACAATAC TGGCGAGAAT 
TCTGATAGGA GACCCTCTAT TCCAAGGAGC TCCAGCATTA TAAGCATTTC TTCGAATGTG 
GCGGAATTTA TGTATAGCAG GAACGAAAAC TCTTTATTTA GAATACACTT GGAATATGGG 
ATTGATGAAG ACAGGCTCAA ATGGTCTATT ATAAGAAGTT ATAAAGATAT CAAATCACTA 
CATCACAAAT TGAAAATCGT CGCGTTCCAA CAACTCACTA TAAGCAAGCT TTATAGTGAT 
AACAACAGAT ATCATTCGTT ACAACTTCCG CATTTCCCCC ATTATAAAGA AATGGTTAAA 
GAAAGAAATG TAATGGAGAA AAAAGCAGAA AATAAACCAT CCTCTGCAGC CTCAGCTCCT 
CATACATCGG AAAATAACAA TAACGATAAT GGCAGCAACA TTACATCGTT AGAAACTTTA 
TCATCTTCTG AAATATCTGA GTTTAATATA GATAACGTCA AAATGAAACA TTTACAAGAC 
TTAATTGACG AACCTGACGA TTTCTCCCAA CCGATACATT TGAGGCTAGA AAGGTATTTG 
AGACTACTGA ACATTGCATT ATGTTTGAGA CCTCATGCAA ATAGATTGTT TGAATTTTAC 
GAATTATCTC CCTTAGGCAA TTTATTAAGT CGTGAAAGTG GGTTCCAGGG CAAACAGGGG 
TATTTAGTAA TTAGATCTAC TGCCAAGGCC CAAGGTTGGA GAGTTTCTCA TTTTGGTAAA 
CATGCGTTTA AAGACATGAT CGACAGACAT ACTACCAAAT GGTTCCTGGT AAGAAATTCT 
TACCTAACTT ACGTGTCGGA TCTTTCTTCG ACAACTCCTT TAGACGTATT CTTAATAGAT 
TGGAAATTTA AGGTTAGATT TTCTGGGAAC AAGAATAACA TACTTGATAA CGAAAACGAG 
ATCAACTGGA TCATTCATGA TCCCAATTTA GAAATAAATG ACGAACTAGA AGAGTTCGGT 
ATTGAAAATG ATGCCAATAA TATCCTAGAC AAGAATGGAA AATCGAAAAC ACACCAAAAG 
AAGTCCAATA TATCTTCAAA ACTATTATTA TTGACCCTAG AAAACAGTGA AAGGAAACTG 
AAAATTATTT GCAAATCAGA GAGTTCGTTA AAGCAATGGA TGAGTTCAAT TATCAAAATG 
TCTACCTCAA CACCTTGGTC GAAACCAAAT AGATTTGGTA GTTTCGCACC TGTAAGAACA 
AATTCGTTTT GCAAATTTTT GGTAGATGGG CGTGATTATT TTTGGTCGTT AAGCGAGGCC 
CTCTTAATGG CAAAGGATGT CATATATATC CATGATTGGT GGCTATCCCC TGAATTATAC 
TTGCGACGTC CGGTGAAAGG CAACCAGGGA TTTAGAATTG ATCGTATGCT GAAAAGTTGT 
GCAGAAAAAG GTATCAAAAT TTTTATTGTG ATTTATAGGA ATGTGGGGAA TATTGTTGGG 
ACAGATAGTC TTTGGACCAA GCATTCTATG CTAAATTTAC ATCCAAATAT ACATATCATA 
AGGTCTCCCA ATCAATGGCT ACAAAATACA TATTTTTGGG CACACCACGA GAAGTTTGTC 
GTTATTGATG AAACATTTGC ATTTATTGGT GGTACAGATT TATGTTATGG TAGATATGAT 
ACTTTTGAAC ACGTTCTACG AGATGATGCA GAATCTTTAT TAGATCAAAA TTTTCCTGGT 
AAGGATTATT CAAATGCCAG AATTGCTGAT TTTCATGATT TAGATAAACC ATTCGAATCA 
ATGTATGATA GAAAAGTCAT TCCGAGGATG CCATGGCATG ATGTGCAAAT GATGACTTTG 
GGTGAGCCAG CAAGAGACTT AGCCCGACAT TTTGTTCAAA GATGGAATTA CTTATTGAGA 
GCTAAGAGAC CTAGTAGGTT AACTCCATTA CTAACCCCGC CTTCAGATTT AACTGCTGAA 
GAATTGAAGA GTTTGCCGAT GTTCGAAATT CTACGCGAAA AATCGACTTG TGAAACTCAA 
ATACTTAGAA GTGCTGGGAA CTGGTCTCTT GGGCTAAAGG AAACGGAATG CTCAATTCAA 
AATGCTTACC TGAAGTTAAT TGAACAAAGT GAACACTTTA TTTATATTGA GAATCAATTC 
TTCATTACAT CAACTGTCTG GAACGGGACT TGCGTTTTGA ATAAAATTGG TGATGCTCTC 
GTTGATAGGA TAGTCAAAGC TAATCAGGAG AAAAAACCTT GGAAAGCCTT TATTCTTATC 
CCACTAATGC CAGGATTCGA TTCTCCAGTA GATACTGCAG AAGCCTCAAG TCTTCGTTTA 
ATTATGCAAT TCCAATATCA ATCAATTTCA AGAGGTGAAC ATTCTACATT TAGTAAACTG 
AAGAAACTAA ATATTGACCC AGCTCAATAT ATTCAGTTCT TTTCACTAAG AAAATGGTCG 
ACTTTTGCGC CTAATGAAAG ATTAATCACC GAGCAGCTCT ACGTTCATGC TAAAATTCTG 
ATTGCTGATG ACAGAAGATG TATTATAGGG TCTGCTAATA TTAACGAAAG ATCACAACTG 
GGTAATAGAG ATAGTGAGGT GGCAATTCTT ATTAGAGACA CCGACTTGAT AAAGACCAAA 
ATGAATGGTG ACGATTACTA CGCGGGAAAA TTTCCTTGGG AATTAAGGCA GCGATTAATG 
AGGGAACATT TAGGTTGTGA TGTAGATCTT GTTGAGTTTG TTGAAAAGAA ATTTGAAAGG 
TTTGAAAAGT TTGCTGCAAA GAATTATGAA AAACTTCATA CCCTAAGTAA GGAGGGAGAT 
AGTGGTAACA ATTGGAGCGA TCGAGAAATG ATCGATTCCG CTATGATTGA GCTAGGTTAT 
CGTGAAATAT TTGGTTGTAA ATTTAGCCCT CAATGGAAGA GTGGTCATGG AAACAGTGTT 
GATGATGGAA GCACTCAGTG TGGCATAAAT GAAAAAGAAG TGGGAAGAGA AGATGAAAAC 
GTTTATGAAA AATTCTTCAA TTCTGTGGAC TACGGTAAAT CATCACGTAA GAGAACGCCT 
CTGCCCAAAC ATAATTTTGC ATCTTTAGGG CTAACATTCA ACCACAGGGC AGGAATTGAA 
AATGTCGGTA TACGAGACCA CAAAGTCTTA AGTACTGATC CAAGATTAAG AAAGAATGAT 
GAACATAAAA AAGAGGTTGA TGGCTACGGT CCCGATTGTT GGAAAAAGGA ATCCAACAAA 
AAATTCAAGG CCGATGCAAC TGAACAGCTA AAGGAGTGGG CATTGAACTC ATTGGCCTCG 
AAAGTTCTTG ACGATAAAGA AATGATAAAA TCAGAAATTC CAGAAGGATT TTCAAATTAT 
TTGCCCAATG AGAAAGATCT AGAAATGTAT CTCACGGATA AAACTGTTAC CAATAGAAAC 
AAATGGAGTA TGCTCAAAAG GATATGCTAC TTGCAATATT TATCTCATAA ACTCGACGAA 
AGAAAAACAC AGAGATTGAA GAAAATCAAA GATATGAGAA GACATTTAAG TTCAAGCACA 
GAAAGTACCA GAAATGGGTC TAATAGTCTG CCATTAAACG AAAAGAGCAA TGAAGGAGAA 
AGCACTAATG TCGACCAAGA TATCGAAGGC GATGAATATC ATAGATTGCA CGAAGATATT 
TTGAAAAATC AAGAACTAGA TGATGGTTCA CTGGACGATC TACTTTCTCA GATAATTCCT 
AAGATCACTA ACTTTAACAG TGGTGAAATT GATGACGCAA AAAAAGAAGA GTTATTGAAA 
CTCAACTTCA TTGACCCGTA TTCCTTCGAG GATCCTCTTA TTTCTTCGTT TTCTGAAGGG 
CTATGGTTCA CTATAGCCTT GAGAAATACA TTACTCTATA AATTAGTGTT TCATTGTCAA 
CCCGATAATG CTGTACAAAA TTGGAAAGAA TATGGAGAAT TTACTGAATT AGAACAAGAA 
TTTCAAATAA ACCAGGAAAA GTTGATTGAC TTAGAGGCTG AAAATATTAA TTCTACTACT 
ACAAATGTTG TAGATAAGGA TAGGGAAAAG GAAAAAATGA GAAAAGCCGC TGAATTGAGA 
ATGAAGTTAT CCGGTAGTTT GCTTTATGGA TTCAATCAAA AGGTTTTTGA CAAACATACG 
GCGCAGAGAA TCTTGGAAAG GATCCACGGT CATTTGGTCA TTTTCCCAAC CGAATGGTTA 
GCTAAAGAAG TTGAGAGTAG AAATTGGATT TTTAACTCGG ATAGACTTTC TCCAATGGAA 
ATCTACAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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SPO14 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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