IRS4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	IRS4
Entrez Gene ID	853889
Full Name	Irs4p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179809.3	NP_012944.3	Irs4p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Irs4p and Tax4p are two redundant modulators of the autophagic processes acting upstream from Atg17p, possibly in the signaling events leading to the activation of the autophagic machinery in response to starvation.
Title: Irs4p and Tax4p: two redundant EH domain proteins involved in autophagy.

The following IRS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03076	NM_001179809.3 Latest version!	Saccharomyces cerevisiae S288C Irs4p (IRS4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRS4 ( NM_001179809.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012944.3 Saccharomyces cerevisiae S288C Irs4p (IRS4), partial mRNA.

Price & Availability↑

CloneID	OSi03076
Clone ID Related Accession (Same CDS sequence)	NM_001179809.3
Accession Version	NM_001179809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Irs4p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179809.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGACTTT CGTTTGGTAA ACAGCGTTAT CATGGCGGTA CAACAGTAAC TTTGACTGAA 
CAAGGAGCTT CAGATTCCCT TCGCGCTGCG CAAGCTATCT TCCAGAACCA TTCCAATGAG 
GTCAGCAGTC CATGCCCTCC AGTAACGGTG AGCAGGAATC CTCAAACTAG GTTATCAGAG 
CCATCGCTAC AAAAGTCAGG GCGGAAACAA GAACAAAAGA AGGCACGCAT ACGAACAAAA 
CAGGTACCTA AGATTAAAAC AACGGCGCCT AATGATGTTG AGTTGTCTAA GAAACACAGG 
TCAAGCCCTG CAGGTAAAGA TAATGTTAGT AGTACTGCTC AAATGGCAGC TGCTTTAGCG 
CATTCACAAT CGAAACTTAG TTCTGACAAT AACTCCTCCC ATTCTTCGGC ATTGGATACA 
CTAAAAGTTC TAGAAACTCC GAACTTGAAT GGCTTGCTGG GCATTCATTC ACGCTCAAGC 
TCAAGAAACG GCTCCAATGA ATCACTGACG CCTGGCCAAA GAACTCCCGA CAATAGATCT 
CAAGAGAATT TACTTACTTC CTTTAGTAGC GGTAGAAGGC TATCCTCGAG CTCTATGGAG 
CCTGCAACTA ATAAAGACAG CAACAAAGCT CTGCCCAAAC GCAGGCCGTC TCCTCCCTTG 
CAATCATCAC TTGTCGGCAG TGGTCAGCTT CATGAAAATG AAAACCTATC ATCAATTAGC 
ATCGATTCCA GACATAGTCT CAATCCGGAC ACTTCAGACG TTATTTCTAA TAGGTCACAA 
ACAAGTCTAT CTCAAACAAT TAATCAACTC TCGTTATGTG AATCAGAGCC ATCTATTGCA 
AGCTCAAACA CTACTACTAC AACGAGCAAC CAGGGAAGTG GTTTGCCCAA CTTGGTTCCG 
AACTATTCAT CCGATATGAG GAAGAAGAAG CTTGTTAACA AGTTCAAAAG GAAAGTATTT 
GGTAGTAAGC CCAAGCACTT ATCTTCACAA TATGAAATGG ATGCAAGCAG CGAAGAACTG 
GGACAACATG AACAACAACC GAGTATGAGA TTTAAAACAA CATTACGTAA AACCTCTGTA 
AGTACAAACG CAGAAAATGA TCATGCATCC TCTCTACATG AGGGAAATCT GAGGTATAAG 
TACAATCCGT CCAACGACAC GTATGATGTG TATGACGATA CTGATTCTGA TTCTGAATCT 
GATCAGAACC AGGACGCCTT GACCAAGCCA CGCAAAAGAG ATAGAATTAA GAGAAAGATC 
AGGAATTCTG CCAATAAAAC TGCACATCAT AGGCCTATCC ACCGCACGAG AGATCGGAAA 
TTCAATGAGG ATAAACCCTG GAAATCACAT ACAGATATAA CTTTCGTAAC AGACAACGAA 
AGGAAGCGTT ACGAAAGCAT GTGGGTAAGC AACAGACACC GTCATTTGAA CCTGTTGAGC 
TGGTGGCCCT CTATAACGGG TGATTCCGGA GCAATTAATA CTTTACCCGA AGATGGATTG 
ATATTGGGTA TCATAGTCCG TGATATCTGG AAGCGGTCTA ACTTGCCCAA TAGTCTATTG 
GCAGAAATTT ACACGAAGGT AGACACAAGA AAAGATGGTA CTTTAGATAG GAAATCTTTT 
ATTGTGGGTA TGTGGCTGGT CGATCAGTGC CTGTATGGCA GAAAACTTCC CAATGTTGTC 
GAGCAATGCG TTTGGGATAG CGTAGATAGA TATGCTTCCA CCACGGTTGT GCCAGTGAGT 
ACGCTAAAGG CGATGGCCAA ACAAAAGAGA AAACAGATGA AAGAAGAAAT CAAGAATATC 
AAGAAGGAAA ACCGTGTAGT TCTTGTAGAC CACAATAGCT CGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012944.3
CDS	1..1848
Translation	MRLSFGKQRYHGGTTVTLTEQGASDSLRAAQAIFQNHSNEVSSPCPPVTVSRNPQTRLSEPSLQKSGRKQEQKKARIRTKQVPKIKTTAPNDVELSKKHRSSPAGKDNVSSTAQMAAALAHSQSKLSSDNNSSHSSALDTLKVLETPNLNGLLGIHSRSSSRNGSNESLTPGQRTPDNRSQENLLTSFSSGRRLSSSSMEPATNKDSNKALPKRRPSPPLQSSLVGSGQLHENENLSSISIDSRHSLNPDTSDVISNRSQTSLSQTINQLSLCESEPSIASSNTTTTTSNQGSGLPNLVPNYSSDMRKKKLVNKFKRKVFGSKPKHLSSQYEMDASSEELGQHEQQPSMRFKTTLRKTSVSTNAENDHASSLHEGNLRYKYNPSNDTYDVYDDTDSDSESDQNQDALTKPRKRDRIKRKIRNSANKTAHHRPIHRTRDRKFNEDKPWKSHTDITFVTDNERKRYESMWVSNRHRHLNLLSWWPSITGDSGAINTLPEDGLILGIIVRDIWKRSNLPNSLLAEIYTKVDTRKDGTLDRKSFIVGMWLVDQCLYGRKLPNVVEQCVWDSVDRYASTTVVPVSTLKAMAKQKRKQMKEEIKNIKKENRVVLVDHNSSS

Target ORF information:

RefSeq Version	NM_001179809.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Irs4p (IRS4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179809.3

ORF Insert Sequence:

ATGAGACTTT CGTTTGGTAA ACAGCGTTAT CATGGCGGTA CAACAGTAAC TTTGACTGAA 
CAAGGAGCTT CAGATTCCCT TCGCGCTGCG CAAGCTATCT TCCAGAACCA TTCCAATGAG 
GTCAGCAGTC CATGCCCTCC AGTAACGGTG AGCAGGAATC CTCAAACTAG GTTATCAGAG 
CCATCGCTAC AAAAGTCAGG GCGGAAACAA GAACAAAAGA AGGCACGCAT ACGAACAAAA 
CAGGTACCTA AGATTAAAAC AACGGCGCCT AATGATGTTG AGTTGTCTAA GAAACACAGG 
TCAAGCCCTG CAGGTAAAGA TAATGTTAGT AGTACTGCTC AAATGGCAGC TGCTTTAGCG 
CATTCACAAT CGAAACTTAG TTCTGACAAT AACTCCTCCC ATTCTTCGGC ATTGGATACA 
CTAAAAGTTC TAGAAACTCC GAACTTGAAT GGCTTGCTGG GCATTCATTC ACGCTCAAGC 
TCAAGAAACG GCTCCAATGA ATCACTGACG CCTGGCCAAA GAACTCCCGA CAATAGATCT 
CAAGAGAATT TACTTACTTC CTTTAGTAGC GGTAGAAGGC TATCCTCGAG CTCTATGGAG 
CCTGCAACTA ATAAAGACAG CAACAAAGCT CTGCCCAAAC GCAGGCCGTC TCCTCCCTTG 
CAATCATCAC TTGTCGGCAG TGGTCAGCTT CATGAAAATG AAAACCTATC ATCAATTAGC 
ATCGATTCCA GACATAGTCT CAATCCGGAC ACTTCAGACG TTATTTCTAA TAGGTCACAA 
ACAAGTCTAT CTCAAACAAT TAATCAACTC TCGTTATGTG AATCAGAGCC ATCTATTGCA 
AGCTCAAACA CTACTACTAC AACGAGCAAC CAGGGAAGTG GTTTGCCCAA CTTGGTTCCG 
AACTATTCAT CCGATATGAG GAAGAAGAAG CTTGTTAACA AGTTCAAAAG GAAAGTATTT 
GGTAGTAAGC CCAAGCACTT ATCTTCACAA TATGAAATGG ATGCAAGCAG CGAAGAACTG 
GGACAACATG AACAACAACC GAGTATGAGA TTTAAAACAA CATTACGTAA AACCTCTGTA 
AGTACAAACG CAGAAAATGA TCATGCATCC TCTCTACATG AGGGAAATCT GAGGTATAAG 
TACAATCCGT CCAACGACAC GTATGATGTG TATGACGATA CTGATTCTGA TTCTGAATCT 
GATCAGAACC AGGACGCCTT GACCAAGCCA CGCAAAAGAG ATAGAATTAA GAGAAAGATC 
AGGAATTCTG CCAATAAAAC TGCACATCAT AGGCCTATCC ACCGCACGAG AGATCGGAAA 
TTCAATGAGG ATAAACCCTG GAAATCACAT ACAGATATAA CTTTCGTAAC AGACAACGAA 
AGGAAGCGTT ACGAAAGCAT GTGGGTAAGC AACAGACACC GTCATTTGAA CCTGTTGAGC 
TGGTGGCCCT CTATAACGGG TGATTCCGGA GCAATTAATA CTTTACCCGA AGATGGATTG 
ATATTGGGTA TCATAGTCCG TGATATCTGG AAGCGGTCTA ACTTGCCCAA TAGTCTATTG 
GCAGAAATTT ACACGAAGGT AGACACAAGA AAAGATGGTA CTTTAGATAG GAAATCTTTT 
ATTGTGGGTA TGTGGCTGGT CGATCAGTGC CTGTATGGCA GAAAACTTCC CAATGTTGTC 
GAGCAATGCG TTTGGGATAG CGTAGATAGA TATGCTTCCA CCACGGTTGT GCCAGTGAGT 
ACGCTAAAGG CGATGGCCAA ACAAAAGAGA AAACAGATGA AAGAAGAAAT CAAGAATATC 
AAGAAGGAAA ACCGTGTAGT TCTTGTAGAC CACAATAGCT CGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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IRS4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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