TOF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TOF2
Entrez Gene ID	853880
Full Name	Tof2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179800.3	NP_012935.3	Tof2p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ctf4-dependent recruitment of Dna2/Tof2 couples other processes to DNA synthesis, including rDNA copy-number regulation.
Title: Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.

These data support a model whereby Tof2 coordinates the biphasic release of Cdc14 during anaphase by restraining a population of Cdc14 in the nucleolus after activation of the Cdc14 early anaphase release (FEAR) network, for subsequent release by the MEN.
Title: Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit.

collaborates with Cdc14 specifically in rDNA segregation, presumably by targeting Cdc14 phosphatase activity to the nucleolus during anaphase to support resolution and compaction of this repetitive and highly transcribed DNA locus
Title: A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis.

The following TOF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03068	NM_001179800.3 Latest version!	Saccharomyces cerevisiae S288C Tof2p (TOF2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOF2 ( NM_001179800.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012935.3 Saccharomyces cerevisiae S288C Tof2p (TOF2), partial mRNA.

Price & Availability↑

CloneID	OSi03068
Clone ID Related Accession (Same CDS sequence)	NM_001179800.3
Accession Version	NM_001179800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Tof2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179800.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATAAAAA TGTGGAGGTT ACAGATTGTA CTGGTGCCGC CAAGTGCTCA AGATATTATC 
ACATTTCTCG AAGCACGCTT GAATACTCCA CAATCAGTGT CTCCTATGGT CCAGTATAAT 
GAAGATATAA TTACGCACAA CAACAGTATC AATAACTGTA GCGACCCCTC TCCTACGAGC 
CCGTCTTCGC AAAATTCTAT TCAGTCGAAT CGTAGTAGTG ATTTCATTAA CTACCTCCCG 
AATTGTAAAA AGTTTTTACA TTTTACGGAC GGTGACAATA CCCTTTTACA ACTATCTAAT 
GAAATTTTAA CTAAATTTGA TAGACTTTAT CCAAATTTCA AAGAATCCAT TGAAATCGTA 
TCTCTACAAG ATAGGCATGG TTGTGATTTG GACTCTGAAT TTATAATAAA AGACGTATTT 
GAAAATGATG GTGTGGTTTT GGTAATTTTA AAAGATGAAT TAGACTGGAG TAGAAATCAA 
CATATTTCAC TTTTACAATT GGCAAGACAG CGGAGACGGC AGGACAACAA GCCATCCACA 
AAATCCATCG TTACTGAAAA GAGAAAAAAG ATCTCTAAGG AAGACTTATC TTCTATATCA 
AATAAAGATA CAATGCATCT AATAGCTAAA AGCTCGCTCA AGAATAACTT CATTAATAAA 
TCAAGAGTAT CAACGCCGCT AATGAACGAA ATTCTACCGC TTGCAAGCAA GTATGATGCA 
CTGAATAAAG AAAAGTGTCC CATGCCACTT ACTTCCACAG TAGTGGCATC AAATGTACAC 
AAAGACGTAA AAGATCACGC TCGTGCTAAA GAGGGAGTTG TAACGCAAGG GAGTGATAAC 
AATAAAGAAA ATATACCTTC ATCCACTCAA CAACAGAAAA ATGATGGCGC TAAAAGGGCC 
GAATCAAAGG ATTTAGATCT TTTACGCAAT TCGTCAGAAG ATGCTGATTA TGAACCCGCA 
GATGAAAACT CCCCTCAAAT TTCGTTTGAT TCGATTGATA CCGACTTCCA ATTGAGCACT 
ACTTCTCATA CTAATTCTGA TATGCATATA CAATATTCTA ACCCCTCTAG TGGTGCTCAT 
AGTCCCAGGA AGAGTTCCTT AGAAATTAAA GTTCAAAACA AAAAAGGTGA TGATCTGCCA 
TTGAACGACA AAGATATAGG TGAGAATTGT AGAAGGATAG AAGCATTTTC AGATGAAGAG 
GATTTTAATG AAACCGATAA TGATCGTGCG GATTCGTTTA TCAACAATTC TAAGAAGGCA 
TCGATGGGAT TTCGGGATAT CAATTCCGAC TTGGATAGCG TTTCATTCAA CAGTGATATT 
GAAAATGCAG TACAAAGTAC TCAAAGTACC AAAAATGTTG TTTCTCCTCC CTTTTTTCCT 
GAAAAAGAAT TGAATAATCG GTTACATCAA AGTCAAGGTA AGGAAGCCCT ATTTCGACTT 
GTTGAGAAGG AGTTTCCTGA TAAATCATTG GGTGCTGCGT CATCTACTTC GCATGCAAAA 
GACGTAAAAA TACAAGAAAC TATTCGGAAA CTAAATAGGT TCAAGCCAAC TGGGGAAACT 
AAGGTCCAAA AAAGGAATAG TATTACGGAA CCATATTACG GAAAATTTGG AATAATGAAG 
AAAGATAAAC CCAAATCCAT AACTTCAAAA GGGGTAAGTC TGGAAACAAA GCACTTTGAT 
GATCCCAATA CCATTATTTC CGGTGAGAAA TTCGCAAAAT TTGGTAAAAT AAAAGTGAAA 
CGAAAAACTG ATGATGTTGG ATCGAAAGTT ATAGAGTTTA AAAGAAAAAG AAATATGGGG 
AATAGATCCC TAAAAGATAT TTTTGCCAAT GCTGGGAAAC CGCCAAATGC AGCTTCGACC 
ATAAAAGTAG TCAAATTAAT GAGAGATCCT GTTGATAATA GTAAAGACAA AGTGGAGGCA 
ACTTCTAATA GTACGGCTCA GGAACAAGAA CAAGTCTCAC CCAAGTTGCC AGTTATGAAT 
TCAACTCCTG GAAAAAGAAA AAATGGCCAA GCGATACCGT CCAGTTTAGA AAGAACACCG 
CAGCTCAAGA AAGTCAAGGT TACCAGGTCG CATTCTTCAC CTTCTTCTTC TTCTTCCATG 
TCACTTGAAT CTTCATTGGA TAGCTCTTCC TCAGATGATT CGGATGATGA TTCTGATTCG 
AGGAACGTGC AAGTTAAAAA AATTAATTTC AAAACAAGTC ATGGACCAGC TGGAAATTCT 
AATGGAAAAC CAATGCTGGA TGTGGATGAC AATGAAATAA ATACAAAGAA GTACCAAACA 
CCTAAATATG TTGAAAGTGA TGAAGACGAC CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012935.3
CDS	1..2316
Translation	MIKMWRLQIVLVPPSAQDIITFLEARLNTPQSVSPMVQYNEDIITHNNSINNCSDPSPTSPSSQNSIQSNRSSDFINYLPNCKKFLHFTDGDNTLLQLSNEILTKFDRLYPNFKESIEIVSLQDRHGCDLDSEFIIKDVFENDGVVLVILKDELDWSRNQHISLLQLARQRRRQDNKPSTKSIVTEKRKKISKEDLSSISNKDTMHLIAKSSLKNNFINKSRVSTPLMNEILPLASKYDALNKEKCPMPLTSTVVASNVHKDVKDHARAKEGVVTQGSDNNKENIPSSTQQQKNDGAKRAESKDLDLLRNSSEDADYEPADENSPQISFDSIDTDFQLSTTSHTNSDMHIQYSNPSSGAHSPRKSSLEIKVQNKKGDDLPLNDKDIGENCRRIEAFSDEEDFNETDNDRADSFINNSKKASMGFRDINSDLDSVSFNSDIENAVQSTQSTKNVVSPPFFPEKELNNRLHQSQGKEALFRLVEKEFPDKSLGAASSTSHAKDVKIQETIRKLNRFKPTGETKVQKRNSITEPYYGKFGIMKKDKPKSITSKGVSLETKHFDDPNTIISGEKFAKFGKIKVKRKTDDVGSKVIEFKRKRNMGNRSLKDIFANAGKPPNAASTIKVVKLMRDPVDNSKDKVEATSNSTAQEQEQVSPKLPVMNSTPGKRKNGQAIPSSLERTPQLKKVKVTRSHSSPSSSSSMSLESSLDSSSSDDSDDDSDSRNVQVKKINFKTSHGPAGNSNGKPMLDVDDNEINTKKYQTPKYVESDEDDQ

Target ORF information:

RefSeq Version	NM_001179800.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Tof2p (TOF2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179800.3

ORF Insert Sequence:

ATGATAAAAA TGTGGAGGTT ACAGATTGTA CTGGTGCCGC CAAGTGCTCA AGATATTATC 
ACATTTCTCG AAGCACGCTT GAATACTCCA CAATCAGTGT CTCCTATGGT CCAGTATAAT 
GAAGATATAA TTACGCACAA CAACAGTATC AATAACTGTA GCGACCCCTC TCCTACGAGC 
CCGTCTTCGC AAAATTCTAT TCAGTCGAAT CGTAGTAGTG ATTTCATTAA CTACCTCCCG 
AATTGTAAAA AGTTTTTACA TTTTACGGAC GGTGACAATA CCCTTTTACA ACTATCTAAT 
GAAATTTTAA CTAAATTTGA TAGACTTTAT CCAAATTTCA AAGAATCCAT TGAAATCGTA 
TCTCTACAAG ATAGGCATGG TTGTGATTTG GACTCTGAAT TTATAATAAA AGACGTATTT 
GAAAATGATG GTGTGGTTTT GGTAATTTTA AAAGATGAAT TAGACTGGAG TAGAAATCAA 
CATATTTCAC TTTTACAATT GGCAAGACAG CGGAGACGGC AGGACAACAA GCCATCCACA 
AAATCCATCG TTACTGAAAA GAGAAAAAAG ATCTCTAAGG AAGACTTATC TTCTATATCA 
AATAAAGATA CAATGCATCT AATAGCTAAA AGCTCGCTCA AGAATAACTT CATTAATAAA 
TCAAGAGTAT CAACGCCGCT AATGAACGAA ATTCTACCGC TTGCAAGCAA GTATGATGCA 
CTGAATAAAG AAAAGTGTCC CATGCCACTT ACTTCCACAG TAGTGGCATC AAATGTACAC 
AAAGACGTAA AAGATCACGC TCGTGCTAAA GAGGGAGTTG TAACGCAAGG GAGTGATAAC 
AATAAAGAAA ATATACCTTC ATCCACTCAA CAACAGAAAA ATGATGGCGC TAAAAGGGCC 
GAATCAAAGG ATTTAGATCT TTTACGCAAT TCGTCAGAAG ATGCTGATTA TGAACCCGCA 
GATGAAAACT CCCCTCAAAT TTCGTTTGAT TCGATTGATA CCGACTTCCA ATTGAGCACT 
ACTTCTCATA CTAATTCTGA TATGCATATA CAATATTCTA ACCCCTCTAG TGGTGCTCAT 
AGTCCCAGGA AGAGTTCCTT AGAAATTAAA GTTCAAAACA AAAAAGGTGA TGATCTGCCA 
TTGAACGACA AAGATATAGG TGAGAATTGT AGAAGGATAG AAGCATTTTC AGATGAAGAG 
GATTTTAATG AAACCGATAA TGATCGTGCG GATTCGTTTA TCAACAATTC TAAGAAGGCA 
TCGATGGGAT TTCGGGATAT CAATTCCGAC TTGGATAGCG TTTCATTCAA CAGTGATATT 
GAAAATGCAG TACAAAGTAC TCAAAGTACC AAAAATGTTG TTTCTCCTCC CTTTTTTCCT 
GAAAAAGAAT TGAATAATCG GTTACATCAA AGTCAAGGTA AGGAAGCCCT ATTTCGACTT 
GTTGAGAAGG AGTTTCCTGA TAAATCATTG GGTGCTGCGT CATCTACTTC GCATGCAAAA 
GACGTAAAAA TACAAGAAAC TATTCGGAAA CTAAATAGGT TCAAGCCAAC TGGGGAAACT 
AAGGTCCAAA AAAGGAATAG TATTACGGAA CCATATTACG GAAAATTTGG AATAATGAAG 
AAAGATAAAC CCAAATCCAT AACTTCAAAA GGGGTAAGTC TGGAAACAAA GCACTTTGAT 
GATCCCAATA CCATTATTTC CGGTGAGAAA TTCGCAAAAT TTGGTAAAAT AAAAGTGAAA 
CGAAAAACTG ATGATGTTGG ATCGAAAGTT ATAGAGTTTA AAAGAAAAAG AAATATGGGG 
AATAGATCCC TAAAAGATAT TTTTGCCAAT GCTGGGAAAC CGCCAAATGC AGCTTCGACC 
ATAAAAGTAG TCAAATTAAT GAGAGATCCT GTTGATAATA GTAAAGACAA AGTGGAGGCA 
ACTTCTAATA GTACGGCTCA GGAACAAGAA CAAGTCTCAC CCAAGTTGCC AGTTATGAAT 
TCAACTCCTG GAAAAAGAAA AAATGGCCAA GCGATACCGT CCAGTTTAGA AAGAACACCG 
CAGCTCAAGA AAGTCAAGGT TACCAGGTCG CATTCTTCAC CTTCTTCTTC TTCTTCCATG 
TCACTTGAAT CTTCATTGGA TAGCTCTTCC TCAGATGATT CGGATGATGA TTCTGATTCG 
AGGAACGTGC AAGTTAAAAA AATTAATTTC AAAACAAGTC ATGGACCAGC TGGAAATTCT 
AATGGAAAAC CAATGCTGGA TGTGGATGAC AATGAAATAA ATACAAAGAA GTACCAAACA 
CCTAAATATG TTGAAAGTGA TGAAGACGAC CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

TOF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報