VPS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS1
Entrez Gene ID	853870
Full Name	dynamin-like GTPase VPS1
Synonyms	GRD1,LAM1,SPO15,VPL1,VPT26
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179791.1	NP_012926.1	dynamin-like GTPase VPS1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	vps1	XP_001713062.1
Arabidopsis thaliana (thale cress)	DRP3A	NP_567931.1
Danio rerio (zebrafish)	si:dkey-32e23.4	XP_005167422.1
Magnaporthe oryzae (rice blast fungus)	MGG_09517	XP_003712225.1
Neurospora crassa	NCU04100	XP_960862.1
Oryza sativa (rice)	Os01g0920400	NP_001045220.1
Arabidopsis thaliana (thale cress)	DRP3B	NP_001154516.1
Saccharomyces cerevisiae (baker's yeast)	VPS1	NP_012926.1
Kluyveromyces lactis	KLLA0B13277g	XP_452123.1
Eremothecium gossypii	AGOS_ABL001W	NP_982946.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data support a model in which phosphorylation and dephosphorylation of Vps1 promote distinct interactions and highlight the importance of such regulatory events in facilitating sequential progression of the endocytic process.
Title: Phosphorylation Regulates the Endocytic Function of the Yeast Dynamin-Related Protein Vps1.

A model is proposed in which actin filaments bind Vps1 during invagination, and this interaction is important to transduce the force of actin polymerization to the membrane to drive successful scission.
Title: A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast.

Fission of retromer SNX-BAR-coated tubules from yeast endosomes is promoted by Vps1.
Title: Fission of SNX-BAR-coated endosomal retrograde transport carriers is promoted by the dynamin-related protein Vps1.

Vps1 functions as a recycling factor in the membrane traffic.
Title: Vps1, a recycling factor for the traffic from early endosome to the late Golgi.

Vps1 is required for correct and efficient protein trafficking from the late endosomal compartments to the vacuole.
Title: Vps1 in the late endosome-to-vacuole traffic.

The following VPS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03059	NM_001179791.1 Latest version!	Saccharomyces cerevisiae S288C dynamin-like GTPase VPS1 (VPS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS1 ( NM_001179791.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012926.1 Saccharomyces cerevisiae S288C dynamin-like GTPase VPS1 (VPS1), partial mRNA.

Price & Availability↑

CloneID	OSi03059
Clone ID Related Accession (Same CDS sequence)	NM_001179791.1
Accession Version	NM_001179791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	dynamin-like GTPase VPS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGAGC ATTTAATTTC TACTATTAAC AAGCTTCAGG ACGCTTTGGC GCCCTTAGGA 
GGAGGATCTC AATCTCCTAT TGATTTACCA CAGATCACTG TTGTCGGTTC CCAGTCGTCA 
GGAAAGTCGT CCGTTTTGGA GAACATTGTT GGTAGGGATT TCTTGCCAAG AGGTACTGGT 
ATTGTCACCA GGAGACCTTT AGTGTTACAA TTGATTAATA GGAGACCAAA AAAGTCAGAA 
CATGCTAAAG TAAACCAAAC TGCTAATGAA TTGATTGACT TGAACATCAA CGATGATGAC 
AAGAAAAAGG ATGAATCAGG AAAGCACCAG AACGAGGGAC AATCTGAAGA CAATAAAGAG 
GAATGGGGTG AATTTTTGCA TTTACCTGGT AAGAAGTTTT ATAATTTTGA CGAAATTAGA 
AAGGAAATCG TCAAAGAAAC TGACAAAGTG ACAGGTGCCA ATTCAGGTAT TTCTTCTGTG 
CCCATTAACT TGAGAATTTA TTCTCCGCAT GTTCTTACTT TGACGTTAGT GGATTTGCCT 
GGGTTGACGA AGGTTCCCGT AGGTGACCAA CCTCCTGATA TTGAAAGACA AATTAAGGAC 
ATGTTGTTAA AGTATATTTC GAAACCAAAC GCTATCATAT TATCTGTTAA TGCCGCTAAC 
ACCGATTTAG CCAACAGCGA TGGTTTGAAG CTGGCTAGAG AGGTCGATCC AGAAGGAACG 
AGAACTATTG GTGTCTTGAC AAAAGTCGAT TTGATGGATC AAGGTACAGA TGTCATAGAT 
ATTTTGGCTG GAAGAGTCAT TCCTTTGAGA TATGGTTATA TCCCAGTTAT CAATAGAGGT 
CAAAAGGATA TTGAACACAA AAAAACAATC AGAGAAGCCC TTGAAAACGA AAGAAAATTT 
TTTGAGAACC ATCCCTCTTA CAGTTCTAAA GCTCATTACT GTGGTACACC ATATTTGGCT 
AAAAAGTTAA ACTCAATCTT ATTACACCAC ATTAGGCAAA CTCTGCCAGA AATCAAAGCG 
AAAATCGAAG CCACATTGAA AAAATATCAA AACGAACTTA TAAACTTGGG CCCAGAAACT 
ATGGATTCAG CTAGTTCGGT TGTTTTGAGC ATGATTACTG ATTTTTCCAA TGAATATGCC 
GGTATCTTGG ACGGTGAGGC GAAGGAGCTT TCCAGTCAGG AACTTTCTGG TGGTGCTAGA 
ATTTCTTACG TATTCCATGA AACTTTCAAA AATGGTGTAG ACTCTTTGGA TCCATTCGAC 
CAGATCAAAG ATTCTGATAT CAGAACCATT ATGTACAATA GTTCAGGTTC TGCCCCATCT 
TTGTTTGTCG GTACCGAAGC TTTTGAAGTT TTAGTTAAAC AGCAAATTAG AAGATTTGAA 
GAACCATCTC TACGTTTAGT TACTCTGGTG TTTGATGAAC TTGTTCGTAT GCTAAAACAG 
ATTATTTCAC AACCAAAGTA CTCAAGGTAT CCTGCTCTAA GAGAAGCGAT TTCTAATCAG 
TTCATTCAGT TCTTAAAGGA TGCTACTATT CCTACGAATG AGTTTGTTGT CGATATCATC 
AAAGCTGAAC AAACTTACAT CAATACAGCC CATCCCGACC TTTTGAAGGG TTCTCAAGCA 
ATGGTTATGG TGGAAGAAAA ATTACATCCT CGCCAAGTCG CTGTTGACCC AAAGACGGGT 
AAACCATTAC CAACCCAACC ATCGTCTAGT AAGGCGCCAG TTATGGAAGA GAAATCAGGA 
TTTTTTGGTG GGTTCTTCTC CACTAAAAAC AAGAAGAAAT TGGCAGCTTT GGAATCCCCA 
CCTCCTGTTT TAAAAGCTAC TGGCCAAATG ACAGAGAGGG AAACAATGGA AACAGAAGTA 
ATCAAGTTGT TGATTAGTAG TTATTTCTCT ATTGTCAAAA GAACCATTGC CGATATTATA 
CCAAAGGCTT TGATGCTTAA ATTGATTGTG AAAAGTAAAA CTGATATTCA GAAAGTTTTA 
CTCGAAAAAC TTTACGGAAA GCAAGATATT GAAGAATTAA CGAAAGAAAA CGACATAACC 
ATTCAAAGAA GAAAAGAATG TAAGAAGATG GTCGAGATAT TGAGAAACGC TAGTCAAATC 
GTCTCCTCTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012926.1
CDS	1..2115
Translation	MDEHLISTINKLQDALAPLGGGSQSPIDLPQITVVGSQSSGKSSVLENIVGRDFLPRGTGIVTRRPLVLQLINRRPKKSEHAKVNQTANELIDLNINDDDKKKDESGKHQNEGQSEDNKEEWGEFLHLPGKKFYNFDEIRKEIVKETDKVTGANSGISSVPINLRIYSPHVLTLTLVDLPGLTKVPVGDQPPDIERQIKDMLLKYISKPNAIILSVNAANTDLANSDGLKLAREVDPEGTRTIGVLTKVDLMDQGTDVIDILAGRVIPLRYGYIPVINRGQKDIEHKKTIREALENERKFFENHPSYSSKAHYCGTPYLAKKLNSILLHHIRQTLPEIKAKIEATLKKYQNELINLGPETMDSASSVVLSMITDFSNEYAGILDGEAKELSSQELSGGARISYVFHETFKNGVDSLDPFDQIKDSDIRTIMYNSSGSAPSLFVGTEAFEVLVKQQIRRFEEPSLRLVTLVFDELVRMLKQIISQPKYSRYPALREAISNQFIQFLKDATIPTNEFVVDIIKAEQTYINTAHPDLLKGSQAMVMVEEKLHPRQVAVDPKTGKPLPTQPSSSKAPVMEEKSGFFGGFFSTKNKKKLAALESPPPVLKATGQMTERETMETEVIKLLISSYFSIVKRTIADIIPKALMLKLIVKSKTDIQKVLLEKLYGKQDIEELTKENDITIQRRKECKKMVEILRNASQIVSSV

Target ORF information:

RefSeq Version	NM_001179791.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C dynamin-like GTPase VPS1 (VPS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179791.1

ORF Insert Sequence:

ATGGATGAGC ATTTAATTTC TACTATTAAC AAGCTTCAGG ACGCTTTGGC GCCCTTAGGA 
GGAGGATCTC AATCTCCTAT TGATTTACCA CAGATCACTG TTGTCGGTTC CCAGTCGTCA 
GGAAAGTCGT CCGTTTTGGA GAACATTGTT GGTAGGGATT TCTTGCCAAG AGGTACTGGT 
ATTGTCACCA GGAGACCTTT AGTGTTACAA TTGATTAATA GGAGACCAAA AAAGTCAGAA 
CATGCTAAAG TAAACCAAAC TGCTAATGAA TTGATTGACT TGAACATCAA CGATGATGAC 
AAGAAAAAGG ATGAATCAGG AAAGCACCAG AACGAGGGAC AATCTGAAGA CAATAAAGAG 
GAATGGGGTG AATTTTTGCA TTTACCTGGT AAGAAGTTTT ATAATTTTGA CGAAATTAGA 
AAGGAAATCG TCAAAGAAAC TGACAAAGTG ACAGGTGCCA ATTCAGGTAT TTCTTCTGTG 
CCCATTAACT TGAGAATTTA TTCTCCGCAT GTTCTTACTT TGACGTTAGT GGATTTGCCT 
GGGTTGACGA AGGTTCCCGT AGGTGACCAA CCTCCTGATA TTGAAAGACA AATTAAGGAC 
ATGTTGTTAA AGTATATTTC GAAACCAAAC GCTATCATAT TATCTGTTAA TGCCGCTAAC 
ACCGATTTAG CCAACAGCGA TGGTTTGAAG CTGGCTAGAG AGGTCGATCC AGAAGGAACG 
AGAACTATTG GTGTCTTGAC AAAAGTCGAT TTGATGGATC AAGGTACAGA TGTCATAGAT 
ATTTTGGCTG GAAGAGTCAT TCCTTTGAGA TATGGTTATA TCCCAGTTAT CAATAGAGGT 
CAAAAGGATA TTGAACACAA AAAAACAATC AGAGAAGCCC TTGAAAACGA AAGAAAATTT 
TTTGAGAACC ATCCCTCTTA CAGTTCTAAA GCTCATTACT GTGGTACACC ATATTTGGCT 
AAAAAGTTAA ACTCAATCTT ATTACACCAC ATTAGGCAAA CTCTGCCAGA AATCAAAGCG 
AAAATCGAAG CCACATTGAA AAAATATCAA AACGAACTTA TAAACTTGGG CCCAGAAACT 
ATGGATTCAG CTAGTTCGGT TGTTTTGAGC ATGATTACTG ATTTTTCCAA TGAATATGCC 
GGTATCTTGG ACGGTGAGGC GAAGGAGCTT TCCAGTCAGG AACTTTCTGG TGGTGCTAGA 
ATTTCTTACG TATTCCATGA AACTTTCAAA AATGGTGTAG ACTCTTTGGA TCCATTCGAC 
CAGATCAAAG ATTCTGATAT CAGAACCATT ATGTACAATA GTTCAGGTTC TGCCCCATCT 
TTGTTTGTCG GTACCGAAGC TTTTGAAGTT TTAGTTAAAC AGCAAATTAG AAGATTTGAA 
GAACCATCTC TACGTTTAGT TACTCTGGTG TTTGATGAAC TTGTTCGTAT GCTAAAACAG 
ATTATTTCAC AACCAAAGTA CTCAAGGTAT CCTGCTCTAA GAGAAGCGAT TTCTAATCAG 
TTCATTCAGT TCTTAAAGGA TGCTACTATT CCTACGAATG AGTTTGTTGT CGATATCATC 
AAAGCTGAAC AAACTTACAT CAATACAGCC CATCCCGACC TTTTGAAGGG TTCTCAAGCA 
ATGGTTATGG TGGAAGAAAA ATTACATCCT CGCCAAGTCG CTGTTGACCC AAAGACGGGT 
AAACCATTAC CAACCCAACC ATCGTCTAGT AAGGCGCCAG TTATGGAAGA GAAATCAGGA 
TTTTTTGGTG GGTTCTTCTC CACTAAAAAC AAGAAGAAAT TGGCAGCTTT GGAATCCCCA 
CCTCCTGTTT TAAAAGCTAC TGGCCAAATG ACAGAGAGGG AAACAATGGA AACAGAAGTA 
ATCAAGTTGT TGATTAGTAG TTATTTCTCT ATTGTCAAAA GAACCATTGC CGATATTATA 
CCAAAGGCTT TGATGCTTAA ATTGATTGTG AAAAGTAAAA CTGATATTCA GAAAGTTTTA 
CTCGAAAAAC TTTACGGAAA GCAAGATATT GAAGAATTAA CGAAAGAAAA CGACATAACC 
ATTCAAAGAA GAAAAGAATG TAAGAAGATG GTCGAGATAT TGAGAAACGC TAGTCAAATC 
GTCTCCTCTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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VPS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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