UFD4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UFD4
Entrez Gene ID	853859
Full Name	putative ubiquitin-protein ligase UFD4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179576.3	NP_012915.3	putative ubiquitin-protein ligase UFD4

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UFD4	NP_012915.3
Magnaporthe oryzae (rice blast fungus)	MGG_03841	XP_003720049.1
Kluyveromyces lactis	KLLA0B13585g	XP_452136.1
Neurospora crassa	NCU09866	XP_960183.1
Schizosaccharomyces pombe (fission yeast)	SPAC12B10.01c	NP_594633.2
Eremothecium gossypii	AGOS_ABR013W	NP_982959.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rad25 turnover requires the ubiquitin-conjugating enzyme Ubc4 and the ubiquitin ligase Ufd4.
Title: Rad25 protein is targeted for degradation by the Ubc4-Ufd4 pathway.

Data suggest that Ufd4 assembles the K29-linked ubiquitin chain(s) on Ub-P-betagal in vivo.
Title: The parallel reaction monitoring method contributes to a highly sensitive polyubiquitin chain quantification.

Study shows that the RING-type Ubr1 E3 and the HECT-type Ufd4 E3 interact, both physically and functionally.
Title: The N-end rule pathway is mediated by a complex of the RING-type Ubr1 and HECT-type Ufd4 ubiquitin ligases.

Armadillo repeats of Ufd4, the E3 component of the UFD (Ub fusion degradation) pathway, recognize the Ub degradation signal of the UFD substrates.
Title: The armadillo repeats of the Ufd4 ubiquitin ligase recognize ubiquitin-fusion proteins.

Ufd4, the E3 component of the UFD pathway, is involved in controlling the degradation of Rad4, and Ufd4 and Rad23 exhibit a synthetic inhibitory effect on Rad4 degradation
Title: A synthetic defect in protein degradation caused by loss of Ufd4 and Rad23.

The following UFD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UFD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03048	NM_001179576.3 Latest version!	Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase UFD4 (UFD4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UFD4 ( NM_001179576.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012915.3 Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase UFD4 (UFD4), partial mRNA.

Price & Availability↑

CloneID	OSi03048
Clone ID Related Accession (Same CDS sequence)	NM_001179576.3
Accession Version	NM_001179576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative ubiquitin-protein ligase UFD4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179576.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAAA ATAATTCGCA CAACCTTGAT GAACATGAGT CCCATAGCGA AAACAGTGAT 
TATATGATGG ATACGCAGGT AGAAGATGAC TATGATGAGG ATGGCCATGT ACAGGGTGAG 
TACTCTTATT ATCCTGATGA AGATGAAGAT GAACATATGC TTTCTAGCGT CGGAAGTTTT 
GAGGCAGATG ATGGTGAAGA TGACGATAAC GATTACCATC ATGAAGATGA TTCTGGACTT 
TTATATGGAT ATCATAGAAC TCAGAATGGC AGTGACGAAG ACAGAAATGA AGAAGAAGAT 
GGACTTGAAC GTTCTCACGA TAATAATGAA TTTGGCAGCA ACCCCCTACA TTTACCTGAC 
ATTTTGGAAA CATTCGCACA AAGACTAGAA CAAAGAAGAC AAACAAGTGA AGGACTTGGG 
CAACACCCGG TTGGAAGAAC ACTACCCGAG ATTTTATCGA TGATTGGAGG AAGGATGGAG 
AGGAGCGCAG AGAGTTCGGC AAGGAATGAG CGGATTTCTA AATTGATAGA GAATACTGGG 
AATGCCTCCG AGGATCCTTA TATTGCAATG GAGAGTTTAA AAGAACTTTC TGAAAACATA 
TTAATGATGA ATCAAATGGT TGTCGATAGA ATTATACCGA TGGAAACCTT AATAGGTAAT 
ATAGCTGCCA TACTCTCTGA TAAAATTTTA CGGGAAGAAT TAGAATTACA AATGCAAGCT 
TGTAGATGCA TGTATAATCT TTTTGAGGTC TGCCCTGAAT CTATTTCAAT AGCTGTTGAT 
GAACACGTTA TACCAATTTT ACAAGGAAAA TTGGTAGAGA TCAGTTACAT TGACCTCGCA 
GAACAAGTTT TAGAAACGGT GGAATATATT TCTAGAGTAC ATGGGAGAGA CATTTTAAAA 
ACGGGCCAAT TATCAATCTA CGTCCAATTC TTCGATTTTT TAACTATACA TGCGCAGAGG 
AAGGCTATCG CAATTGTTTC GAACGCCTGT AGCAGTATCC GAACGGATGA CTTTAAGACC 
ATTGTTGAAG TACTTCCAAC GCTGAAGCCA ATTTTCTCGA ATGCGACAGA CCAACCAATA 
TTAACCAGGC TTGTAAATGC CATGTACGGT ATTTGCGGGG CGTTGCATGG GGTTGACAAA 
TTTGAGACTT TGTTTTCGTT GGATTTAATC GAAAGAATAG TTCAGCTAGT TTCTATTCAG 
GATACCCCCT TGGAGAATAA ACTGAAATGT TTGGATATTT TAACCGTATT AGCGATGAGT 
AGTGATGTAC TTTCAAGAGA ACTGAGAGAG AAAACTGACA TTGTCGACAT GGCAACACGA 
TCATTCCAGC ATTATAGTAA AAGTCCTAAC GCAGGGTTAC ATGAAACACT GATTTATGTC 
CCAAACAGTT TATTGATTAG CATTTCTAGA TTTATAGTTG TATTGTTTCC TCCCGAGGAT 
GAAAGAATAC TGTCAGCGGA TAAATATACC GGAAATAGCG ACCGTGGCGT AATTTCTAAC 
CAGGAAAAGT TTGATTCCCT AGTTCAATGT CTAATTCCAA TTCTCGTTGA AATTTATACA 
AATGCTGCTG ACTTTGACGT AAGAAGATAC GTACTTATTG CTTTACTGAG GGTTGTATCA 
TGCATAAATA ATTCCACAGC AAAGGCAATC AATGATCAAC TTATTAAGTT AATCGGATCT 
ATCCTGGCCC AAAAAGAAAC AGCGTCTAAC GCTAATGGTA CTTACTCATC AGAAGCTGGT 
ACACTGTTGG TTGGTGGTCT CTCGTTGCTT GACTTAATTT GTAAAAAGTT TTCCGAACTG 
TTCTTTCCTT CCATCAAAAG AGAGGGCATT TTTGATTTGG TTAAGGATTT GTCTGTGGAT 
TTCAATAACA TTGATTTAAA GGAAGACGGG AATGAAAATA TTTCACTTTC TGACGAAGAA 
GGGGATTTGC ATAGCAGTAT TGAGGAATGT GATGAGGGTG ATGAAGAATA TGATTACGAA 
TTTACTGATA TGGAAATTCC TGATTCAGTA AAACCAAAGA AAATTTCAAT ACACATTTTC 
AGAACTCTAT CTCTAGCTTA TATTAAAAAC AAGGGTGTGA ACCTAGTTAA TAGAGTACTT 
TCTCAGATGA ACGTTGAGCA AGAAGCAATA ACAGAGGAGC TCCATCAAAT CGAAGGCGTT 
GTTTCTATTT TAGAAAATCC TTCCACTCCG GACAAAACTG AAGAGGATTG GAAGGGAATT 
TGGTCTGTTT TAAAAAAATG TATTTTCCAT GAAGATTTCG ACGTGTCAGG TTTCGAATTT 
ACTTCTACAG GGCTAGCTTC CTCCATAACT AAAAGAATTA CATCCTCAAC GGTATCCCAT 
TTCATTCTTG CTAAATCATT TTTAGAGGTA TTTGAGGATT GTATTGACAG ATTTTTAGAA 
ATCCTACAAT CTGCTCTCAC AAGGCTGGAG AATTTCTCTA TAGTTGATTG CGGTTTACAC 
GATGGTGGTG GTGTATCTTC ACTGGCTAAA GAGATAAAAA TTAAGTTGGT TTATGATGGC 
GATGCAAGCA AAGATAATAT TGGTACTGAT TTATCATCTA CTATCGTTTC GGTCCATTGC 
ATAGCTTCTT TTACCTCACT TAATGAGTTT TTGAGACACA GAATGGTAAG AATGCGTTTT 
TTGAATTCAT TAATCCCAAA CCTTACATCT TCCAGTACCG AAGCTGATAG GGAAGAAGAA 
GAAAATTGCT TGGATCATAT GAGAAAAAAG AACTTTGACT TTTTTTATGA TAATGAAAAA 
GTTGACATGG AGTCTACAGT ATTTGGTGTG ATATTTAATA CATTCGTCAG GCGAAATCGT 
GACTTAAAAA CTTTATGGGA TGATACACAT ACAATCAAAT TTTGCAAAAG TTTAGAAGGT 
AACAATAGAG AGAGTGAGGC AGCCGAGGAA GCTAATGAGG GGAAAAAGTT AAGAGATTTT 
TATAAAAAAA GAGAATTCGC ACAGGTTGAT ACTGGATCTT CAGCGGATAT TCTGACATTG 
CTGGATTTTC TACATAGCTG CGGTGTTAAA AGCGACAGTT TTATCAACTC AAAACTAAGC 
GCTAAGCTCG CTAGACAACT AGATGAACCA TTGGTAGTAG CAAGTGGAGC TTTGCCGGAT 
TGGTCACTAT TTTTGACCAG GAGATTCCCA TTTTTGTTTC CGTTTGATAC CAGGATGCTT 
TTCCTACAAT GTACTTCATT TGGTTACGGA AGATTGATTC AACTTTGGAA GAATAAGAGT 
AAAGGCTCGA AAGATTTAAG GAATGACGAA GCTTTACAAC AACTTGGGAG AATTACTAGG 
CGTAAGCTGC GGATTTCAAG AAAAACAATA TTCGCTACCG GTCTCAAGAT TTTATCCAAG 
TACGGAAGTA GCCCTGACGT ACTGGAAATT GAATATCAAG AAGAAGCAGG AACAGGTTTA 
GGACCGACTT TGGAATTTTA CTCCGTAGTT TCCAAGTATT TTGCAAGAAA GTCGTTAAAT 
ATGTGGCGTT GTAACTCTTA TAGTTACAGA AGCGAAATGG ATGTTGATAC TACTGACGAT 
TATATTACCA CTTTATTGTT CCCAGAGCCC CTCAACCCGT TTTCCAATAA TGAAAAAGTT 
ATTGAACTTT TTGGATATTT GGGGACATTT GTTGCCAGAT CGTTGCTTGA TAATAGAATT 
CTTGACTTTA GATTTAGCAA AGTCTTTTTT GAGTTATTGC ACAGAATGTC TACGCCCAAT 
GTGACGACAG TGCCGAGCGA CGTTGAAACC TGTCTGTTAA TGATCGAATT GGTAGATCCG 
TTACTCGCAA AATCCCTTAA ATACATAGTA GCGAATAAGG ATGACAATAT GACCCTAGAA 
TCGTTGTCCT TGACATTTAC CGTTCCTGGA AATGATGACA TTGAGTTGAT TCCGGGGGGT 
TGTAATAAAT CCTTGAACTC TTCTAATGTT GAAGAATATA TCCATGGCGT TATCGACCAA 
ATATTAGGTA AGGGCATTGA AAAACAGTTA AAAGCATTTA TTGAAGGTTT TTCAAAGGTG 
TTTTCCTATG AGAGGATGCT AATACTTTTT CCGGATGAAT TAGTGGATAT TTTCGGACGA 
GTTGAGGAAG ACTGGTCTAT GGCAACTTTA TACACAAACT TGAACGCTGA ACATGGCTAT 
ACAATGGATT CTTCAATCAT TCATGATTTT ATATCAATAA TATCCGCGTT TGGTAAGCAT 
GAAAGAAGAT TATTTTTGCA ATTTTTAACG GGATCTCCCA AGCTTCCAAT TGGGGGATTT 
AAAAGTTTGA ACCCAAAGTT TACAGTTGTG TTAAAGCATG CTGAAGATGG CCTAACAGCA 
GACGAATATC TACCAAGTGT AATGACATGT GCTAATTATT TGAAATTGCC GAAGTATACT 
AGCAAAGATA TTATGCGGTC TCGTCTTTGT CAAGCCATTG AAGAGGGTGC AGGAGCTTTT 
CTACTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012915.3
CDS	1..4452
Translation	MSENNSHNLDEHESHSENSDYMMDTQVEDDYDEDGHVQGEYSYYPDEDEDEHMLSSVGSFEADDGEDDDNDYHHEDDSGLLYGYHRTQNGSDEDRNEEEDGLERSHDNNEFGSNPLHLPDILETFAQRLEQRRQTSEGLGQHPVGRTLPEILSMIGGRMERSAESSARNERISKLIENTGNASEDPYIAMESLKELSENILMMNQMVVDRIIPMETLIGNIAAILSDKILREELELQMQACRCMYNLFEVCPESISIAVDEHVIPILQGKLVEISYIDLAEQVLETVEYISRVHGRDILKTGQLSIYVQFFDFLTIHAQRKAIAIVSNACSSIRTDDFKTIVEVLPTLKPIFSNATDQPILTRLVNAMYGICGALHGVDKFETLFSLDLIERIVQLVSIQDTPLENKLKCLDILTVLAMSSDVLSRELREKTDIVDMATRSFQHYSKSPNAGLHETLIYVPNSLLISISRFIVVLFPPEDERILSADKYTGNSDRGVISNQEKFDSLVQCLIPILVEIYTNAADFDVRRYVLIALLRVVSCINNSTAKAINDQLIKLIGSILAQKETASNANGTYSSEAGTLLVGGLSLLDLICKKFSELFFPSIKREGIFDLVKDLSVDFNNIDLKEDGNENISLSDEEGDLHSSIEECDEGDEEYDYEFTDMEIPDSVKPKKISIHIFRTLSLAYIKNKGVNLVNRVLSQMNVEQEAITEELHQIEGVVSILENPSTPDKTEEDWKGIWSVLKKCIFHEDFDVSGFEFTSTGLASSITKRITSSTVSHFILAKSFLEVFEDCIDRFLEILQSALTRLENFSIVDCGLHDGGGVSSLAKEIKIKLVYDGDASKDNIGTDLSSTIVSVHCIASFTSLNEFLRHRMVRMRFLNSLIPNLTSSSTEADREEEENCLDHMRKKNFDFFYDNEKVDMESTVFGVIFNTFVRRNRDLKTLWDDTHTIKFCKSLEGNNRESEAAEEANEGKKLRDFYKKREFAQVDTGSSADILTLLDFLHSCGVKSDSFINSKLSAKLARQLDEPLVVASGALPDWSLFLTRRFPFLFPFDTRMLFLQCTSFGYGRLIQLWKNKSKGSKDLRNDEALQQLGRITRRKLRISRKTIFATGLKILSKYGSSPDVLEIEYQEEAGTGLGPTLEFYSVVSKYFARKSLNMWRCNSYSYRSEMDVDTTDDYITTLLFPEPLNPFSNNEKVIELFGYLGTFVARSLLDNRILDFRFSKVFFELLHRMSTPNVTTVPSDVETCLLMIELVDPLLAKSLKYIVANKDDNMTLESLSLTFTVPGNDDIELIPGGCNKSLNSSNVEEYIHGVIDQILGKGIEKQLKAFIEGFSKVFSYERMLILFPDELVDIFGRVEEDWSMATLYTNLNAEHGYTMDSSIIHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSLNPKFTVVLKHAEDGLTADEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAGAFLLS

Target ORF information:

RefSeq Version	NM_001179576.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase UFD4 (UFD4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179576.3

ORF Insert Sequence:

ATGTCTGAAA ATAATTCGCA CAACCTTGAT GAACATGAGT CCCATAGCGA AAACAGTGAT 
TATATGATGG ATACGCAGGT AGAAGATGAC TATGATGAGG ATGGCCATGT ACAGGGTGAG 
TACTCTTATT ATCCTGATGA AGATGAAGAT GAACATATGC TTTCTAGCGT CGGAAGTTTT 
GAGGCAGATG ATGGTGAAGA TGACGATAAC GATTACCATC ATGAAGATGA TTCTGGACTT 
TTATATGGAT ATCATAGAAC TCAGAATGGC AGTGACGAAG ACAGAAATGA AGAAGAAGAT 
GGACTTGAAC GTTCTCACGA TAATAATGAA TTTGGCAGCA ACCCCCTACA TTTACCTGAC 
ATTTTGGAAA CATTCGCACA AAGACTAGAA CAAAGAAGAC AAACAAGTGA AGGACTTGGG 
CAACACCCGG TTGGAAGAAC ACTACCCGAG ATTTTATCGA TGATTGGAGG AAGGATGGAG 
AGGAGCGCAG AGAGTTCGGC AAGGAATGAG CGGATTTCTA AATTGATAGA GAATACTGGG 
AATGCCTCCG AGGATCCTTA TATTGCAATG GAGAGTTTAA AAGAACTTTC TGAAAACATA 
TTAATGATGA ATCAAATGGT TGTCGATAGA ATTATACCGA TGGAAACCTT AATAGGTAAT 
ATAGCTGCCA TACTCTCTGA TAAAATTTTA CGGGAAGAAT TAGAATTACA AATGCAAGCT 
TGTAGATGCA TGTATAATCT TTTTGAGGTC TGCCCTGAAT CTATTTCAAT AGCTGTTGAT 
GAACACGTTA TACCAATTTT ACAAGGAAAA TTGGTAGAGA TCAGTTACAT TGACCTCGCA 
GAACAAGTTT TAGAAACGGT GGAATATATT TCTAGAGTAC ATGGGAGAGA CATTTTAAAA 
ACGGGCCAAT TATCAATCTA CGTCCAATTC TTCGATTTTT TAACTATACA TGCGCAGAGG 
AAGGCTATCG CAATTGTTTC GAACGCCTGT AGCAGTATCC GAACGGATGA CTTTAAGACC 
ATTGTTGAAG TACTTCCAAC GCTGAAGCCA ATTTTCTCGA ATGCGACAGA CCAACCAATA 
TTAACCAGGC TTGTAAATGC CATGTACGGT ATTTGCGGGG CGTTGCATGG GGTTGACAAA 
TTTGAGACTT TGTTTTCGTT GGATTTAATC GAAAGAATAG TTCAGCTAGT TTCTATTCAG 
GATACCCCCT TGGAGAATAA ACTGAAATGT TTGGATATTT TAACCGTATT AGCGATGAGT 
AGTGATGTAC TTTCAAGAGA ACTGAGAGAG AAAACTGACA TTGTCGACAT GGCAACACGA 
TCATTCCAGC ATTATAGTAA AAGTCCTAAC GCAGGGTTAC ATGAAACACT GATTTATGTC 
CCAAACAGTT TATTGATTAG CATTTCTAGA TTTATAGTTG TATTGTTTCC TCCCGAGGAT 
GAAAGAATAC TGTCAGCGGA TAAATATACC GGAAATAGCG ACCGTGGCGT AATTTCTAAC 
CAGGAAAAGT TTGATTCCCT AGTTCAATGT CTAATTCCAA TTCTCGTTGA AATTTATACA 
AATGCTGCTG ACTTTGACGT AAGAAGATAC GTACTTATTG CTTTACTGAG GGTTGTATCA 
TGCATAAATA ATTCCACAGC AAAGGCAATC AATGATCAAC TTATTAAGTT AATCGGATCT 
ATCCTGGCCC AAAAAGAAAC AGCGTCTAAC GCTAATGGTA CTTACTCATC AGAAGCTGGT 
ACACTGTTGG TTGGTGGTCT CTCGTTGCTT GACTTAATTT GTAAAAAGTT TTCCGAACTG 
TTCTTTCCTT CCATCAAAAG AGAGGGCATT TTTGATTTGG TTAAGGATTT GTCTGTGGAT 
TTCAATAACA TTGATTTAAA GGAAGACGGG AATGAAAATA TTTCACTTTC TGACGAAGAA 
GGGGATTTGC ATAGCAGTAT TGAGGAATGT GATGAGGGTG ATGAAGAATA TGATTACGAA 
TTTACTGATA TGGAAATTCC TGATTCAGTA AAACCAAAGA AAATTTCAAT ACACATTTTC 
AGAACTCTAT CTCTAGCTTA TATTAAAAAC AAGGGTGTGA ACCTAGTTAA TAGAGTACTT 
TCTCAGATGA ACGTTGAGCA AGAAGCAATA ACAGAGGAGC TCCATCAAAT CGAAGGCGTT 
GTTTCTATTT TAGAAAATCC TTCCACTCCG GACAAAACTG AAGAGGATTG GAAGGGAATT 
TGGTCTGTTT TAAAAAAATG TATTTTCCAT GAAGATTTCG ACGTGTCAGG TTTCGAATTT 
ACTTCTACAG GGCTAGCTTC CTCCATAACT AAAAGAATTA CATCCTCAAC GGTATCCCAT 
TTCATTCTTG CTAAATCATT TTTAGAGGTA TTTGAGGATT GTATTGACAG ATTTTTAGAA 
ATCCTACAAT CTGCTCTCAC AAGGCTGGAG AATTTCTCTA TAGTTGATTG CGGTTTACAC 
GATGGTGGTG GTGTATCTTC ACTGGCTAAA GAGATAAAAA TTAAGTTGGT TTATGATGGC 
GATGCAAGCA AAGATAATAT TGGTACTGAT TTATCATCTA CTATCGTTTC GGTCCATTGC 
ATAGCTTCTT TTACCTCACT TAATGAGTTT TTGAGACACA GAATGGTAAG AATGCGTTTT 
TTGAATTCAT TAATCCCAAA CCTTACATCT TCCAGTACCG AAGCTGATAG GGAAGAAGAA 
GAAAATTGCT TGGATCATAT GAGAAAAAAG AACTTTGACT TTTTTTATGA TAATGAAAAA 
GTTGACATGG AGTCTACAGT ATTTGGTGTG ATATTTAATA CATTCGTCAG GCGAAATCGT 
GACTTAAAAA CTTTATGGGA TGATACACAT ACAATCAAAT TTTGCAAAAG TTTAGAAGGT 
AACAATAGAG AGAGTGAGGC AGCCGAGGAA GCTAATGAGG GGAAAAAGTT AAGAGATTTT 
TATAAAAAAA GAGAATTCGC ACAGGTTGAT ACTGGATCTT CAGCGGATAT TCTGACATTG 
CTGGATTTTC TACATAGCTG CGGTGTTAAA AGCGACAGTT TTATCAACTC AAAACTAAGC 
GCTAAGCTCG CTAGACAACT AGATGAACCA TTGGTAGTAG CAAGTGGAGC TTTGCCGGAT 
TGGTCACTAT TTTTGACCAG GAGATTCCCA TTTTTGTTTC CGTTTGATAC CAGGATGCTT 
TTCCTACAAT GTACTTCATT TGGTTACGGA AGATTGATTC AACTTTGGAA GAATAAGAGT 
AAAGGCTCGA AAGATTTAAG GAATGACGAA GCTTTACAAC AACTTGGGAG AATTACTAGG 
CGTAAGCTGC GGATTTCAAG AAAAACAATA TTCGCTACCG GTCTCAAGAT TTTATCCAAG 
TACGGAAGTA GCCCTGACGT ACTGGAAATT GAATATCAAG AAGAAGCAGG AACAGGTTTA 
GGACCGACTT TGGAATTTTA CTCCGTAGTT TCCAAGTATT TTGCAAGAAA GTCGTTAAAT 
ATGTGGCGTT GTAACTCTTA TAGTTACAGA AGCGAAATGG ATGTTGATAC TACTGACGAT 
TATATTACCA CTTTATTGTT CCCAGAGCCC CTCAACCCGT TTTCCAATAA TGAAAAAGTT 
ATTGAACTTT TTGGATATTT GGGGACATTT GTTGCCAGAT CGTTGCTTGA TAATAGAATT 
CTTGACTTTA GATTTAGCAA AGTCTTTTTT GAGTTATTGC ACAGAATGTC TACGCCCAAT 
GTGACGACAG TGCCGAGCGA CGTTGAAACC TGTCTGTTAA TGATCGAATT GGTAGATCCG 
TTACTCGCAA AATCCCTTAA ATACATAGTA GCGAATAAGG ATGACAATAT GACCCTAGAA 
TCGTTGTCCT TGACATTTAC CGTTCCTGGA AATGATGACA TTGAGTTGAT TCCGGGGGGT 
TGTAATAAAT CCTTGAACTC TTCTAATGTT GAAGAATATA TCCATGGCGT TATCGACCAA 
ATATTAGGTA AGGGCATTGA AAAACAGTTA AAAGCATTTA TTGAAGGTTT TTCAAAGGTG 
TTTTCCTATG AGAGGATGCT AATACTTTTT CCGGATGAAT TAGTGGATAT TTTCGGACGA 
GTTGAGGAAG ACTGGTCTAT GGCAACTTTA TACACAAACT TGAACGCTGA ACATGGCTAT 
ACAATGGATT CTTCAATCAT TCATGATTTT ATATCAATAA TATCCGCGTT TGGTAAGCAT 
GAAAGAAGAT TATTTTTGCA ATTTTTAACG GGATCTCCCA AGCTTCCAAT TGGGGGATTT 
AAAAGTTTGA ACCCAAAGTT TACAGTTGTG TTAAAGCATG CTGAAGATGG CCTAACAGCA 
GACGAATATC TACCAAGTGT AATGACATGT GCTAATTATT TGAAATTGCC GAAGTATACT 
AGCAAAGATA TTATGCGGTC TCGTCTTTGT CAAGCCATTG AAGAGGGTGC AGGAGCTTTT 
CTACTTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
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UFD4 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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