URB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	URB1
Entrez Gene ID	853855
Full Name	Urb1p
Synonyms	NPA1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179580.3	NP_012911.3	Urb1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E18151g	XP_454769.1
Eremothecium gossypii	AGOS_ACL095C	NP_983309.1
Saccharomyces cerevisiae (baker's yeast)	URB1	NP_012911.3
Schizosaccharomyces pombe (fission yeast)	SPCC14G10.02	NP_587943.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results support the idea that Npa2p, Dbp6p, Npa1p, Nop8p, and Rsa3p work in concert during the early steps of 60S subunit biogenesis.
Title: Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis.

The following URB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the URB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03044	NM_001179580.3 Latest version!	Saccharomyces cerevisiae S288C Urb1p (URB1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

URB1 ( NM_001179580.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012911.3 Saccharomyces cerevisiae S288C Urb1p (URB1), partial mRNA.

Price & Availability↑

CloneID	OSi03044
Clone ID Related Accession (Same CDS sequence)	NM_001179580.3
Accession Version	NM_001179580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Urb1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179580.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTAATC ATAGCGAAGC CTATGGATCT CGCGACCAGA GAAGGGAGAA GTACACCCAA 
GGGAAAGAGT TCGAAGACGG TACATTAGAA ACACTAGAAA GTATAATCAG TGCAGTCGAA 
GATGAAACTC TCTCCAAAGA TTACCAACCT CTAATAGTGT TCTTCCAAAG AGGATTTGGT 
GCTCAATTGG TACAGACTTG GTCATATTAT GCTCAAGTAA ATAACCATGG GAAATTCTCG 
AAAACTACCT CATTACTGAC TAAAACCTTA CGAGTTTTGT CATCTGATAC TAGTACAGTG 
ACTATTGGGA GCGGTCTAAT TAGATTGATA TTGACCGATT ACACAAAAGT TCTCTATAGA 
GGGTTGAACA ATATGCGTGC GCAATTAACT AATCCAATTC TTCGTCTACT CAAACAAATA 
GTCAATTTCA ACAATGGGCA ACATATAGAG GAATTAGTTT CATATTTCGA TTTTTCATTA 
CCTATTTTAC CAAGACTGTT GGTACCAAGC AAGTCTGAGT TAGCCAATGG GAATTCATCT 
GCTGATTCAT CCAAACATGA CTCATTAAGA TTTACATTTA TCAAGTTTTG GTTGACATTG 
ATCTCAAATG CTTCCCCATT CGTGAGAAAA GAACTTTTGA CAGAAAACTT TAAGATTATG 
AGCAACCTAT TTAAATTCAT GAATAAGGCT GATTCTGACA AGTTATCTGA GCACATACTA 
TCGGTATTCA TAAACGACAT TTTAAAGGAG AAATCCTTCA AGAGAACTAC TAAAACCAAA 
ATTTTAAATG AACTAGCGGC TTCAAAGATT CATCACTTTT ATTATTCATC AAACAAGAAT 
TTGGTTAAGA AGGCAAATGA GTTTTTCTTA ACTTTTGGAG CTTCCCGTGA CTTTAGCGTA 
GCATTTCCTG ATAATTGCGT TTGGTTCAAA AATTCAGTTG CTGATGGAGC TTCTCATGGA 
GCTCCGATAA CCGTCAACCA AGTGGAATTT CAAATTCATA ATAAACTTTT GTTCAACACT 
TTACGCCTTT TCAAACCTTG GGAAGATACG CTGCAATTAG GTACGTTGAT CAAAATATTG 
GAGAACGTTC CCGAACTTGT TGCTCCTTAC AGCATCTTTT TAACGACGAA TGGTAACTAT 
GACCCTAAGA TGACTTCGTA TTGGTTTGGT ATAACGTTAC TGATTAACAA GATTATTAAT 
CTTAAGATAC CGCAATTTAT GGAGAAAGTT GATAGCAATA TTCCACCTGC CACTTCATTA 
GTTATAGAAA ACATTCTGCC ATCTTTGCTC ACCAAATCCT CGTTGGTTAA ATCCCTACAA 
TTTGAAACAC CCATCATAAG ACAATTAGCC TGTCAATCAA TAGTTCTAGC GTTAAAAAAA 
TTAGAAAAAG TTTCCACATT TTATGATCAA AAAGGCTGGA GAAATGAAAA AACTATTTTA 
CTCAACGAGT TTCATACTAG AATTCCTGAT TTACCAATCT TCGTATCTAC TTTGAGCAAT 
TCTTTGGCTT CCAATAAGGA TAATAGAATT TTACCTCTGT CCATATCCAT CATATTCAAC 
TACTATAGTA AAATGTTCCC CAACCTCTTC TCAATTAACC TACCATCATC AAATATTTAC 
ACAGATATCA TGCAAAAATC CAAGATTTCT GGCATAGAAT TTGCTATTCT GGACAACTAC 
TTACAATTTC AAGAATTTAA CTCTACTCAA ACCAGGTGGT GGAATCCAAG TTCTGGTGGA 
AATTCTTTAT TCACCCTACT ATTAAAGCTA GCATCCTCCA AAAATGCTTC TAACGTGATA 
ACTACAAGAA TATCCAACTT GCTCGATGAG CTGACGAGAA CAAACGTTAT CTTCAACATA 
TCTTTAATTT CACCAGTGAT GGCTTTAGTG AATAGTCTTC AAGGGCTTTC CCTTCAAGTT 
TCTGAAATTG ACAACATGGA ACAAGTCTGG AAGTGGTTTG ACGAAACTAT ATCTCGTGTC 
GTAAAAACAC CATACAAGTA CGTCGATATG GCAAAAGAGT ATAACTACAT TTCTCCCTTC 
ATAATGTGCC TTTCAGAGCA ATGGAAATAC GTTGACAAGA GTGGAAACCC AGAGTTTTTA 
ATTAAATGGC TTATACTTTT TCTTAGAAAC ATGATTTTCA TTGGTGAAGA CCATATAGGC 
ATAGATAAAC TTGTTAAAAA TGTCTTTCCT GAGGTCTCTG ATCATGATGT TAATATATAC 
TTGAAACTTG ACTCATTTGA AGAAAACATA AAGAAAACTA ATAGCTCTAA TTCTTTAATT 
TCTTCAATGA AGAGCTCATC TTTTTTCCAG TATATTTCGG CGCTTCCATC TAAAAATCTA 
ATGAATATTT CTAGATTACC AGTGAACAAA TTAGATGCGG CAGGAATCCT TTTTAGAGTT 
CAGTTATTGG TAGAAGACGA TTCTGTTGTA TATGACAATT GGTTCGAAGC AACTGCCTGT 
GAGTTAACTG GCAAGATTGC TAGCTACATG GTAACAGACA CAGAATTCCC AATTATCAAA 
GTTTTGGAAA GGTATATAAA TTTCGCTTTA CCAAAACTGG CCATTGAAAA AAGAAATGCT 
CTACTCATGA AAAAATCAAG GTTCATGTGC AATTTAATCG GTGCAGTATG CTTTGAAACA 
GGCCATCAAT TAGTGGAATT CCGAGAAATA ATTCAAAAAG TCGTGTTTAG TGGTGAAAAT 
GTCGAAGAGT ATGCCAACTA TAATGAGTTG TATCAGAAAG AAGATGTAAA TGCTTTCTTA 
ACAAGTGTTA GTGAGTATTT AAGTACTTCA GCTTTAACTT CGCTACTCAT GTGTAGCACC 
AAACTAGAGT CCACAAGAAA CATTCTCCAA AAATTATTCA ACGAAGGAAA AACTATAAAA 
ATATCCCTTG TAAAGAATAT ACTGAACAAA GCTGCAAATG AAGATCCTGC TTCAATTAAG 
GAAGTAAACA TCTCTTTGGC TAAATTTTTT GAAGAAAACA AAGTTTGTGT TGATGCTTCA 
TCTGATCCTA TGGGTAAGCT ATCCCTTTCA GAAACTACTT CTCTGATAAA TAGTTTTGTG 
TCTAGTGATT TGAATTATCT AGTACTGAAA GCTTTTTACA GATGGGAACA TTTTTCTTTC 
CCATCTTTCA TACCAAGTAT CTGGAGGATC AAGGATTCAC CTTTACTAAG TATTGTAACA 
ACGGCTGCTT TGTTCAAGCA CATGCAGGAT AAAGATTTTA GCGCATTTGC GCATGAAACC 
ATTTCCAAGT ATGGTAATGA AATTGCGAAA AGTACTTACA CTACCTCGAA AAGTGAGATT 
TTTGACGAAA TATTAAATAT GATTACCACT TATATTGATT TCTATGACGA AACGAAACGA 
AATGAAATTT TGAAATGTGT TTTATCACAG AGCGACCATA AATATCATGC TGCTACAGTA 
AGATACATAG CTGCACACAA TAATTTTACT TATCCAGGGG TAGAAACATG GTTACATAAA 
ACTCTTCTTT ACCTTACAAA GTACCTTTCA GAAAGAAAAG TTATCTCCAA TTCTTTTTTT 
GAGTTACTAA GAGCGATGGC TGAGCTCTTA AAACTTGAAG AGGTACCAAA TAAGTTGAAT 
GTCAAAATCA TTAATTCTCA ATTAGAGGCT ATTCTAGGCA GTGAGTGGAT CAAACAAATT 
AAGGTTCTTG AATACGTTAT TGTATTAATT TTTTGTGTGA GCAAAAAGTC AATTCAATCG 
CAAAGAATGG TTCAACTTTT ATTAAGCAAT GACAGTTACT CATCAATAAT GATTAAAGAC 
AATGATGAAG ATTCATCGTA TCGAAAGTTC TTAAGCACAA TGATATTATT CTCTTTATTC 
AGCATCGACC CGGTCGTAAA TTCGACGCCA ATAGTTCAAG AGAAATTATT GACTTTTTAC 
TCCGGTACTA TTTCCAGTAA CGACAAGTTG ATACTGAAAA TTCTAGAAAC TATCGAATCT 
CACACCGCTA CTTCATGGAC AAACATGATT TTTTCTTGGG AATTTATCAA AGACGAAGAG 
GAAGAAATCT TGGAAGCCAT TGGAGACACG AGGCTGATTA CCAAAGAAAG GGAGGGGTTG 
ATACTAACAC TACAAAAAAA CATGATCAAA AAGTCCATTG ACAGATACGT ATTGGAAAGG 
CCTCAAGTCC CTGAATTATA TACCGATTCT AATACAAACA ATTATGATGC TACTACAAGG 
TGTGACCTAG TCAAAAAGTA CTACGATGAT ACCGAACGAT CTGGTGTTGA TATGTACGAT 
CCATTATTTT TACTGCTATT AATAATCCAT AACAAAGAGC TGGTAAAAAT GGTCAAAGAT 
GACGAGGGCA ATGTAACATA CAGATATGAA TTTGAAAATT TCCTTGATTG CAAAATATTT 
CAGTTTATTA TTTGCTCATT AAGCGATTGT CATACCGTTG CCAATATAAG TTACGAACAT 
TTATCAAATT TAGCTTCTTC TTTGGAGAAG AAAACTGCCC AGATGAATCT TGAAAAGCAA 
ATAACTAGCA AGGATAATGA AAGAAAAGAA TCTGATAGCG ATTTAATCAA ATATAATAGC 
ATTTACCAAG TGTTGATTAA AAGGATTTTA TACCAGAGAC AACAAAATCA AGATCCTATC 
AATCCGTTAA TTTGGTTTTC TATCTCTAGA ATTGTTGATC TATTGGGAAG TCCAACTGCG 
CCCTTACATG AGAAGGCATA CCGCTGGGTA TTATCAAATT CTACCATACG TAGTTGGGAC 
ATCCCTATGG TATCTGATGT GATGATGTCC TACAATAAGA GACAACAAGA TGACAATAAG 
AAAGAAATTG ATATGGAAAT ATACTATGGA GAATTGAGTT GGGTTTTAAC AACCATTTGT 
AAGGGAATTA AAACGGATGA AGATTACAAG ATGTTAGAGA AGAAAGGTGT ATTTGAATGG 
TTGTTAAACT TGATCAATAT GCCATACTTG AAAGAAAGAT TACGCGAACT AATCTATTTT 
ATTTTTTACA AAGTCCAAAG GGTAGCAGAT GATGGTGGTT TGAATTTAAT TAGCAGAAAC 
GGTATTGTGT CGTTTTTCGA GGTTTTGAAC AATAACATCA AATCTAGACT ACCACAGGAC 
GATATACTGA ACAACATTGG TACTTTACGG AACGAAAACA GAGGTACATT AAATACTACT 
TTGAGGTTAG CCCAAGAACA AAACGGAATT GAAAAACTAC TATTAGGCTA CAACGAGCTT 
GTCAAATCAC AAAAAAGGCT AATATTATGG ACAGAAGGTG ATAGCGACAA TGTTGTCAAG 
AGGCTACGTA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012911.3
CDS	1..5295
Translation	MSNHSEAYGSRDQRREKYTQGKEFEDGTLETLESIISAVEDETLSKDYQPLIVFFQRGFGAQLVQTWSYYAQVNNHGKFSKTTSLLTKTLRVLSSDTSTVTIGSGLIRLILTDYTKVLYRGLNNMRAQLTNPILRLLKQIVNFNNGQHIEELVSYFDFSLPILPRLLVPSKSELANGNSSADSSKHDSLRFTFIKFWLTLISNASPFVRKELLTENFKIMSNLFKFMNKADSDKLSEHILSVFINDILKEKSFKRTTKTKILNELAASKIHHFYYSSNKNLVKKANEFFLTFGASRDFSVAFPDNCVWFKNSVADGASHGAPITVNQVEFQIHNKLLFNTLRLFKPWEDTLQLGTLIKILENVPELVAPYSIFLTTNGNYDPKMTSYWFGITLLINKIINLKIPQFMEKVDSNIPPATSLVIENILPSLLTKSSLVKSLQFETPIIRQLACQSIVLALKKLEKVSTFYDQKGWRNEKTILLNEFHTRIPDLPIFVSTLSNSLASNKDNRILPLSISIIFNYYSKMFPNLFSINLPSSNIYTDIMQKSKISGIEFAILDNYLQFQEFNSTQTRWWNPSSGGNSLFTLLLKLASSKNASNVITTRISNLLDELTRTNVIFNISLISPVMALVNSLQGLSLQVSEIDNMEQVWKWFDETISRVVKTPYKYVDMAKEYNYISPFIMCLSEQWKYVDKSGNPEFLIKWLILFLRNMIFIGEDHIGIDKLVKNVFPEVSDHDVNIYLKLDSFEENIKKTNSSNSLISSMKSSSFFQYISALPSKNLMNISRLPVNKLDAAGILFRVQLLVEDDSVVYDNWFEATACELTGKIASYMVTDTEFPIIKVLERYINFALPKLAIEKRNALLMKKSRFMCNLIGAVCFETGHQLVEFREIIQKVVFSGENVEEYANYNELYQKEDVNAFLTSVSEYLSTSALTSLLMCSTKLESTRNILQKLFNEGKTIKISLVKNILNKAANEDPASIKEVNISLAKFFEENKVCVDASSDPMGKLSLSETTSLINSFVSSDLNYLVLKAFYRWEHFSFPSFIPSIWRIKDSPLLSIVTTAALFKHMQDKDFSAFAHETISKYGNEIAKSTYTTSKSEIFDEILNMITTYIDFYDETKRNEILKCVLSQSDHKYHAATVRYIAAHNNFTYPGVETWLHKTLLYLTKYLSERKVISNSFFELLRAMAELLKLEEVPNKLNVKIINSQLEAILGSEWIKQIKVLEYVIVLIFCVSKKSIQSQRMVQLLLSNDSYSSIMIKDNDEDSSYRKFLSTMILFSLFSIDPVVNSTPIVQEKLLTFYSGTISSNDKLILKILETIESHTATSWTNMIFSWEFIKDEEEEILEAIGDTRLITKEREGLILTLQKNMIKKSIDRYVLERPQVPELYTDSNTNNYDATTRCDLVKKYYDDTERSGVDMYDPLFLLLLIIHNKELVKMVKDDEGNVTYRYEFENFLDCKIFQFIICSLSDCHTVANISYEHLSNLASSLEKKTAQMNLEKQITSKDNERKESDSDLIKYNSIYQVLIKRILYQRQQNQDPINPLIWFSISRIVDLLGSPTAPLHEKAYRWVLSNSTIRSWDIPMVSDVMMSYNKRQQDDNKKEIDMEIYYGELSWVLTTICKGIKTDEDYKMLEKKGVFEWLLNLINMPYLKERLRELIYFIFYKVQRVADDGGLNLISRNGIVSFFEVLNNNIKSRLPQDDILNNIGTLRNENRGTLNTTLRLAQEQNGIEKLLLGYNELVKSQKRLILWTEGDSDNVVKRLRK

Target ORF information:

RefSeq Version	NM_001179580.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Urb1p (URB1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179580.3

ORF Insert Sequence:

ATGAGTAATC ATAGCGAAGC CTATGGATCT CGCGACCAGA GAAGGGAGAA GTACACCCAA 
GGGAAAGAGT TCGAAGACGG TACATTAGAA ACACTAGAAA GTATAATCAG TGCAGTCGAA 
GATGAAACTC TCTCCAAAGA TTACCAACCT CTAATAGTGT TCTTCCAAAG AGGATTTGGT 
GCTCAATTGG TACAGACTTG GTCATATTAT GCTCAAGTAA ATAACCATGG GAAATTCTCG 
AAAACTACCT CATTACTGAC TAAAACCTTA CGAGTTTTGT CATCTGATAC TAGTACAGTG 
ACTATTGGGA GCGGTCTAAT TAGATTGATA TTGACCGATT ACACAAAAGT TCTCTATAGA 
GGGTTGAACA ATATGCGTGC GCAATTAACT AATCCAATTC TTCGTCTACT CAAACAAATA 
GTCAATTTCA ACAATGGGCA ACATATAGAG GAATTAGTTT CATATTTCGA TTTTTCATTA 
CCTATTTTAC CAAGACTGTT GGTACCAAGC AAGTCTGAGT TAGCCAATGG GAATTCATCT 
GCTGATTCAT CCAAACATGA CTCATTAAGA TTTACATTTA TCAAGTTTTG GTTGACATTG 
ATCTCAAATG CTTCCCCATT CGTGAGAAAA GAACTTTTGA CAGAAAACTT TAAGATTATG 
AGCAACCTAT TTAAATTCAT GAATAAGGCT GATTCTGACA AGTTATCTGA GCACATACTA 
TCGGTATTCA TAAACGACAT TTTAAAGGAG AAATCCTTCA AGAGAACTAC TAAAACCAAA 
ATTTTAAATG AACTAGCGGC TTCAAAGATT CATCACTTTT ATTATTCATC AAACAAGAAT 
TTGGTTAAGA AGGCAAATGA GTTTTTCTTA ACTTTTGGAG CTTCCCGTGA CTTTAGCGTA 
GCATTTCCTG ATAATTGCGT TTGGTTCAAA AATTCAGTTG CTGATGGAGC TTCTCATGGA 
GCTCCGATAA CCGTCAACCA AGTGGAATTT CAAATTCATA ATAAACTTTT GTTCAACACT 
TTACGCCTTT TCAAACCTTG GGAAGATACG CTGCAATTAG GTACGTTGAT CAAAATATTG 
GAGAACGTTC CCGAACTTGT TGCTCCTTAC AGCATCTTTT TAACGACGAA TGGTAACTAT 
GACCCTAAGA TGACTTCGTA TTGGTTTGGT ATAACGTTAC TGATTAACAA GATTATTAAT 
CTTAAGATAC CGCAATTTAT GGAGAAAGTT GATAGCAATA TTCCACCTGC CACTTCATTA 
GTTATAGAAA ACATTCTGCC ATCTTTGCTC ACCAAATCCT CGTTGGTTAA ATCCCTACAA 
TTTGAAACAC CCATCATAAG ACAATTAGCC TGTCAATCAA TAGTTCTAGC GTTAAAAAAA 
TTAGAAAAAG TTTCCACATT TTATGATCAA AAAGGCTGGA GAAATGAAAA AACTATTTTA 
CTCAACGAGT TTCATACTAG AATTCCTGAT TTACCAATCT TCGTATCTAC TTTGAGCAAT 
TCTTTGGCTT CCAATAAGGA TAATAGAATT TTACCTCTGT CCATATCCAT CATATTCAAC 
TACTATAGTA AAATGTTCCC CAACCTCTTC TCAATTAACC TACCATCATC AAATATTTAC 
ACAGATATCA TGCAAAAATC CAAGATTTCT GGCATAGAAT TTGCTATTCT GGACAACTAC 
TTACAATTTC AAGAATTTAA CTCTACTCAA ACCAGGTGGT GGAATCCAAG TTCTGGTGGA 
AATTCTTTAT TCACCCTACT ATTAAAGCTA GCATCCTCCA AAAATGCTTC TAACGTGATA 
ACTACAAGAA TATCCAACTT GCTCGATGAG CTGACGAGAA CAAACGTTAT CTTCAACATA 
TCTTTAATTT CACCAGTGAT GGCTTTAGTG AATAGTCTTC AAGGGCTTTC CCTTCAAGTT 
TCTGAAATTG ACAACATGGA ACAAGTCTGG AAGTGGTTTG ACGAAACTAT ATCTCGTGTC 
GTAAAAACAC CATACAAGTA CGTCGATATG GCAAAAGAGT ATAACTACAT TTCTCCCTTC 
ATAATGTGCC TTTCAGAGCA ATGGAAATAC GTTGACAAGA GTGGAAACCC AGAGTTTTTA 
ATTAAATGGC TTATACTTTT TCTTAGAAAC ATGATTTTCA TTGGTGAAGA CCATATAGGC 
ATAGATAAAC TTGTTAAAAA TGTCTTTCCT GAGGTCTCTG ATCATGATGT TAATATATAC 
TTGAAACTTG ACTCATTTGA AGAAAACATA AAGAAAACTA ATAGCTCTAA TTCTTTAATT 
TCTTCAATGA AGAGCTCATC TTTTTTCCAG TATATTTCGG CGCTTCCATC TAAAAATCTA 
ATGAATATTT CTAGATTACC AGTGAACAAA TTAGATGCGG CAGGAATCCT TTTTAGAGTT 
CAGTTATTGG TAGAAGACGA TTCTGTTGTA TATGACAATT GGTTCGAAGC AACTGCCTGT 
GAGTTAACTG GCAAGATTGC TAGCTACATG GTAACAGACA CAGAATTCCC AATTATCAAA 
GTTTTGGAAA GGTATATAAA TTTCGCTTTA CCAAAACTGG CCATTGAAAA AAGAAATGCT 
CTACTCATGA AAAAATCAAG GTTCATGTGC AATTTAATCG GTGCAGTATG CTTTGAAACA 
GGCCATCAAT TAGTGGAATT CCGAGAAATA ATTCAAAAAG TCGTGTTTAG TGGTGAAAAT 
GTCGAAGAGT ATGCCAACTA TAATGAGTTG TATCAGAAAG AAGATGTAAA TGCTTTCTTA 
ACAAGTGTTA GTGAGTATTT AAGTACTTCA GCTTTAACTT CGCTACTCAT GTGTAGCACC 
AAACTAGAGT CCACAAGAAA CATTCTCCAA AAATTATTCA ACGAAGGAAA AACTATAAAA 
ATATCCCTTG TAAAGAATAT ACTGAACAAA GCTGCAAATG AAGATCCTGC TTCAATTAAG 
GAAGTAAACA TCTCTTTGGC TAAATTTTTT GAAGAAAACA AAGTTTGTGT TGATGCTTCA 
TCTGATCCTA TGGGTAAGCT ATCCCTTTCA GAAACTACTT CTCTGATAAA TAGTTTTGTG 
TCTAGTGATT TGAATTATCT AGTACTGAAA GCTTTTTACA GATGGGAACA TTTTTCTTTC 
CCATCTTTCA TACCAAGTAT CTGGAGGATC AAGGATTCAC CTTTACTAAG TATTGTAACA 
ACGGCTGCTT TGTTCAAGCA CATGCAGGAT AAAGATTTTA GCGCATTTGC GCATGAAACC 
ATTTCCAAGT ATGGTAATGA AATTGCGAAA AGTACTTACA CTACCTCGAA AAGTGAGATT 
TTTGACGAAA TATTAAATAT GATTACCACT TATATTGATT TCTATGACGA AACGAAACGA 
AATGAAATTT TGAAATGTGT TTTATCACAG AGCGACCATA AATATCATGC TGCTACAGTA 
AGATACATAG CTGCACACAA TAATTTTACT TATCCAGGGG TAGAAACATG GTTACATAAA 
ACTCTTCTTT ACCTTACAAA GTACCTTTCA GAAAGAAAAG TTATCTCCAA TTCTTTTTTT 
GAGTTACTAA GAGCGATGGC TGAGCTCTTA AAACTTGAAG AGGTACCAAA TAAGTTGAAT 
GTCAAAATCA TTAATTCTCA ATTAGAGGCT ATTCTAGGCA GTGAGTGGAT CAAACAAATT 
AAGGTTCTTG AATACGTTAT TGTATTAATT TTTTGTGTGA GCAAAAAGTC AATTCAATCG 
CAAAGAATGG TTCAACTTTT ATTAAGCAAT GACAGTTACT CATCAATAAT GATTAAAGAC 
AATGATGAAG ATTCATCGTA TCGAAAGTTC TTAAGCACAA TGATATTATT CTCTTTATTC 
AGCATCGACC CGGTCGTAAA TTCGACGCCA ATAGTTCAAG AGAAATTATT GACTTTTTAC 
TCCGGTACTA TTTCCAGTAA CGACAAGTTG ATACTGAAAA TTCTAGAAAC TATCGAATCT 
CACACCGCTA CTTCATGGAC AAACATGATT TTTTCTTGGG AATTTATCAA AGACGAAGAG 
GAAGAAATCT TGGAAGCCAT TGGAGACACG AGGCTGATTA CCAAAGAAAG GGAGGGGTTG 
ATACTAACAC TACAAAAAAA CATGATCAAA AAGTCCATTG ACAGATACGT ATTGGAAAGG 
CCTCAAGTCC CTGAATTATA TACCGATTCT AATACAAACA ATTATGATGC TACTACAAGG 
TGTGACCTAG TCAAAAAGTA CTACGATGAT ACCGAACGAT CTGGTGTTGA TATGTACGAT 
CCATTATTTT TACTGCTATT AATAATCCAT AACAAAGAGC TGGTAAAAAT GGTCAAAGAT 
GACGAGGGCA ATGTAACATA CAGATATGAA TTTGAAAATT TCCTTGATTG CAAAATATTT 
CAGTTTATTA TTTGCTCATT AAGCGATTGT CATACCGTTG CCAATATAAG TTACGAACAT 
TTATCAAATT TAGCTTCTTC TTTGGAGAAG AAAACTGCCC AGATGAATCT TGAAAAGCAA 
ATAACTAGCA AGGATAATGA AAGAAAAGAA TCTGATAGCG ATTTAATCAA ATATAATAGC 
ATTTACCAAG TGTTGATTAA AAGGATTTTA TACCAGAGAC AACAAAATCA AGATCCTATC 
AATCCGTTAA TTTGGTTTTC TATCTCTAGA ATTGTTGATC TATTGGGAAG TCCAACTGCG 
CCCTTACATG AGAAGGCATA CCGCTGGGTA TTATCAAATT CTACCATACG TAGTTGGGAC 
ATCCCTATGG TATCTGATGT GATGATGTCC TACAATAAGA GACAACAAGA TGACAATAAG 
AAAGAAATTG ATATGGAAAT ATACTATGGA GAATTGAGTT GGGTTTTAAC AACCATTTGT 
AAGGGAATTA AAACGGATGA AGATTACAAG ATGTTAGAGA AGAAAGGTGT ATTTGAATGG 
TTGTTAAACT TGATCAATAT GCCATACTTG AAAGAAAGAT TACGCGAACT AATCTATTTT 
ATTTTTTACA AAGTCCAAAG GGTAGCAGAT GATGGTGGTT TGAATTTAAT TAGCAGAAAC 
GGTATTGTGT CGTTTTTCGA GGTTTTGAAC AATAACATCA AATCTAGACT ACCACAGGAC 
GATATACTGA ACAACATTGG TACTTTACGG AACGAAAACA GAGGTACATT AAATACTACT 
TTGAGGTTAG CCCAAGAACA AAACGGAATT GAAAAACTAC TATTAGGCTA CAACGAGCTT 
GTCAAATCAC AAAAAAGGCT AATATTATGG ACAGAAGGTG ATAGCGACAA TGTTGTCAAG 
AGGCTACGTA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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URB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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