RGT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RGT1
Entrez Gene ID	853828
Full Name	Rgt1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179604.3	NP_012886.3	Rgt1p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RGT1	NP_012886.3
Kluyveromyces lactis	KLLA0F25630g	XP_456221.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression.
Title: The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae.

Data show that Pph3-Psy2 targets the glucose signal transducer protein Mth1, which is required for the dephosphorylation of Rgt1 upon glucose withdrawal, a critical event in the repression of HXT genes, which encode glucose transporters.
Title: Psy2 targets the PP4 family phosphatase Pph3 to dephosphorylate Mth1 and repress glucose transporter gene expression.

DNA-binding properties of the yeast RGT1 repressor are reported.
Title: DNA-binding properties of the yeast Rgt1 repressor.

Data show that two different glucose signal transduction pathways converge on Rgt1 to regulate expression of HXT glucose transporters.
Title: Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae.

Rgt1 binds to the RGT1 element of HXK2 promoter in a glucose-dependent manner, and the repression of target gene depends on binding of Rgt1 to DNA.
Title: Rgt1, a glucose sensing transcription factor, is required for transcriptional repression of the HXK2 gene in Saccharomyces cerevisiae.

The following RGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03021	NM_001179604.3 Latest version!	Saccharomyces cerevisiae S288C Rgt1p (RGT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RGT1 ( NM_001179604.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012886.3 Saccharomyces cerevisiae S288C Rgt1p (RGT1), partial mRNA.

Price & Availability↑

CloneID	OSi03021
Clone ID Related Accession (Same CDS sequence)	NM_001179604.3
Accession Version	NM_001179604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rgt1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179604.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACGAGC TGAACACTGT TTCGACTAAC TCCAGTGACT CCACCAAGAA CGGTGGTACA 
TCCAATAGTC CCGACGATAT GGACTCCGCA GCAGCAGCAA GTCACGCAAT AAAGAAAAGA 
ACAAAGGCCT CCCGGGCCTG CGATCAGTGT CGTAAAAAGA AGATTAAATG TGACTACAAA 
GATGAAAAGG GGGTCTGCTC AAACTGCCAA AGGAATGGAG ACCGTTGTAG TTTTGATAGG 
GTTCCTTTGA AAAGGGGACC CTCGAAAGGC TATACGAGGA GCACCAGCCA TCCAAGGACT 
AACGAAATAC AAGACCACAA TAACTCAAGG TCGTATAATA CGTTTGATAA TAGTAACAAT 
ACCCTGAATA ATAATACGGG CAATAGCGGC GACAACGGTA TAAACAGTAA CACAGTTCCA 
AGTACCCCTT CGAGATCTAA TTCAGTTTTA TTGCCCCCAC TGACACAGTA CATTCCCCAG 
GCTGGTGGCA TTCCTCCTAG TTTCCAAAAT CCAGCGATAC AATCAACTAT GCCCGCGGGT 
AATATTGGCC AACAGCAGTT TTGGAAAGTG CCCTACCATG AGTTTCAACA CCAAAGAAAA 
GGATCTATCG ATTCTTTGCA AAGTGATATA TCGGTAAGAA CATTAAATCC CAACGAGCAG 
TTGTCTTATA ACACCGTTCA ACAGTCTCCC ATAACGAATA AACATACTAA TGATTCTGGA 
AATGCAAACG GAAGTGTCAC CGGCTCAGGC AGTGCCTCTG GTAGTGGTGG TTATTGGTCC 
TTTATAAGAA CTTCTGGTTT ACTAGCTCCT ACTGATGATC ATAATGGGGA ACAGACAAGA 
AGATCAAGTT CCATACCTTC TTTGCTACGA AATACTTCAA ATTCTCTTTT ACTAGGTGGC 
CAGCCTCAGC TTCCCCCACC TCAGCAACAA TCGCAGCCAC AAGCACACCA ACAAAAGTTG 
CAACAAGGAC AGAACCTATA CTCGTATTCT CAATTTTCCC AGCAGCAACC GTACAACCCA 
TCGATATCAT CTTTTGGCCA GTTCGCTGCT AATGGTTTTC ATTCTAGACA AGGATCAGTC 
GCCAGCGAGG CTATGTCTCC CAGTGCACCT GCCATGTTTA CTAGCACATC TACAAACCCC 
GTAAATGTTG CACAGCAAAC ACAAAGACCT CAAGGACAGC AGGTACCGCA ATTTTCATCT 
GAGTTGGATG GAAATAAAAG GCGACAATCA GCCCCCGTAT CAGTAACATT ATCCACGGAC 
AGGCTGAATG GTAATGAGAA TAATAATGGT GAAATTAACA ACAATAATGG CAGCAATAAT 
AGCGGTTCTT CTAAGGATAC ATCTCAACAT TCCCAAGAAT CTGTAACTAC ACCAGCGGCT 
TTGGAAGCAT CCAGCCCTGG ATCAACGCCA CAGAGAAGTA CAAAAAAACG CAGAAAAAGT 
TACGTATCTA AGAAGACAAA ACCAAAGAGA GATTCATCTA TATCTATAAC ATCGAAAGAT 
TCTGCTCACC CAATGACCAC TTCATCAACT ATCGCGTATG GACAGATATC CGATGTAGAT 
CTAATAGACA CCTACTATGA GTTCATACAT GTAGGATTTC CGATCATACC TTTAAACAAA 
ACGACCTTGA CCAGTGACTT ATTGTTAGTT AACACACAGC CAATTTCCAA TATACACGAA 
GTCAATTCAT ATGTTATTTT GTGGTTTAGA AATTCATTGG AATTGTTAGT TCGTGTTGCT 
CTGAAACAAA AGCCAGGTGG CAAGTTTTTC GATAATATTG TTGGCGTGGC TTTGTCGCCA 
AGTAATGACA ACAACAAAGC TGGGTTCACT ACAGCCACGG CAAGGGATGA TGCTGAAAAA 
ACAAGACGTG ATTCACATAA TGAAGTACAG GATACTTTGG AAGTGCAAAG CGTTTTTATT 
GCCGCCCTCA ATGAATGTTT CCAAAAAATC GTGGATATTC ATCCCAAATT CAGAGAAAAC 
AACGACCAAA TTTCGCCGAA GATCAAAGTC ATTTATTTAT CTACTTTTAT TCTTTTAAAT 
TACATTTTGG CATTTGTTGG ATACGATAAC TCATTTGTAC TTGGAATGTC GGTGACAATT 
TTTAACGAAT TTAAATTATA CAAACTTCTA TTATTTCCGG AGCCGGATAT AAATGATGTG 
AAGCCTCCAG TTGATGAAGA GGTCAGCACT GGTAATGGGA ATACAAAAAC GTCCGAATTT 
GAAATTGGAT CTGAAAGTGC TGGGCATATG AATCCATCCA ATTCACCAAA TTCCATGGAC 
GAAAACATTA GTCACTATTC AGTGTTGTTT AAAAGATTAT ACGTTTTGCT TTCAGTGTTT 
GATTCTTTAC AAAGCTGTGC ATTCGGTGGT CCCAAGCTAT TAAACATTTC CATCCAAGGT 
TCTACAGAAA GATTTTTTTC TAATGATTTG GGCTCAAAAT GGTGCCTGGA ACAAAGCCAA 
TTGAGACTGA AAAGCGTCTT GCAAAGCTTG AAATTGGGTG AATTGATGAG TGAGCTTACC 
AGGAATAGAA TATCAATGAA CGGCAATAGG AAGCCTGGGT TCGATATTAC GAACTCGTCT 
TCACTCTTAT CGGAATATGT GGAAACTCAA CCTCTATCCG TAGCACAGTT ATTTTGCAAG 
CTATTAATTG GCAAACACAA TTTCATCAAT TGCTTATTAT CATTATACGA TTCAGAAGCA 
GGAGTCTATT CAGATTTGAC ATTGGATTTG AGTTCGAAAA TAGCAGACTC TCTGTGCTCA 
TTGATATCAA TAATTTTGCA AGTATTGACG TTGATATTAA GACTGAATCC TACGAACAGT 
ATTGATTTTA ATTATAGACC ACCGAACCCA CCCGCTAATA ATCCGACAGT GCAAGAGGGC 
CCATCTGCTA TGGGCTCGTC TCCAGTCGCT GGGAACCTTA GCGCTGCACC TCCATCGGAG 
GGGAATCCAG ATTTTTACAA GAAATTACTA GGCCTGAAAC AAGACACTGG CACTATCCTT 
TCAGACCTTT GCCGGGGGAT AATTTCCCCC TTTGCTATTG CTATCCTGCA CGAGGTCTAC 
AACATCACTG AACTGGTCAA GCAGATGCCT ACATCACTCA TTAGCATTAT GATGACGGCA 
ACTACAACGC AGAATACTCA GGACACCAAG AAGTCGCAGG ACCTGGTCAT GAAGCTGTCA 
AACTCGATGA ATGAAGTAGT TCAAATCACC AGCGTGCTGA CAATGATCAA GCCATTCAAG 
ATCTTCGAGC ACGAGCTTAA TAAGCCCATA ATGTCCCTGA CAGGAGGACT TTCGTCCACG 
ACCAGAAACG ACGTCATGTG GCCAAAGTCG GGCCAAGGCC TCCGCGAGTC ATCAGTCATG 
AAGACATTGC TTGATGAACG CCGTACTTCT GGTACACAAC CGACAACGGC GCCAGTAGCC 
GCAGAGGAAC CCAGGCTTGA GAACGTTGCC CTGGAAAATT TCGTTAGTAT CGGCTGGAAG 
CTGTTGGACG ATTCCGAGTT AGGCTGGTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012886.3
CDS	1..3513
Translation	MNELNTVSTNSSDSTKNGGTSNSPDDMDSAAAASHAIKKRTKASRACDQCRKKKIKCDYKDEKGVCSNCQRNGDRCSFDRVPLKRGPSKGYTRSTSHPRTNEIQDHNNSRSYNTFDNSNNTLNNNTGNSGDNGINSNTVPSTPSRSNSVLLPPLTQYIPQAGGIPPSFQNPAIQSTMPAGNIGQQQFWKVPYHEFQHQRKGSIDSLQSDISVRTLNPNEQLSYNTVQQSPITNKHTNDSGNANGSVTGSGSASGSGGYWSFIRTSGLLAPTDDHNGEQTRRSSSIPSLLRNTSNSLLLGGQPQLPPPQQQSQPQAHQQKLQQGQNLYSYSQFSQQQPYNPSISSFGQFAANGFHSRQGSVASEAMSPSAPAMFTSTSTNPVNVAQQTQRPQGQQVPQFSSELDGNKRRQSAPVSVTLSTDRLNGNENNNGEINNNNGSNNSGSSKDTSQHSQESVTTPAALEASSPGSTPQRSTKKRRKSYVSKKTKPKRDSSISITSKDSAHPMTTSSTIAYGQISDVDLIDTYYEFIHVGFPIIPLNKTTLTSDLLLVNTQPISNIHEVNSYVILWFRNSLELLVRVALKQKPGGKFFDNIVGVALSPSNDNNKAGFTTATARDDAEKTRRDSHNEVQDTLEVQSVFIAALNECFQKIVDIHPKFRENNDQISPKIKVIYLSTFILLNYILAFVGYDNSFVLGMSVTIFNEFKLYKLLLFPEPDINDVKPPVDEEVSTGNGNTKTSEFEIGSESAGHMNPSNSPNSMDENISHYSVLFKRLYVLLSVFDSLQSCAFGGPKLLNISIQGSTERFFSNDLGSKWCLEQSQLRLKSVLQSLKLGELMSELTRNRISMNGNRKPGFDITNSSSLLSEYVETQPLSVAQLFCKLLIGKHNFINCLLSLYDSEAGVYSDLTLDLSSKIADSLCSLISIILQVLTLILRLNPTNSIDFNYRPPNPPANNPTVQEGPSAMGSSPVAGNLSAAPPSEGNPDFYKKLLGLKQDTGTILSDLCRGIISPFAIAILHEVYNITELVKQMPTSLISIMMTATTTQNTQDTKKSQDLVMKLSNSMNEVVQITSVLTMIKPFKIFEHELNKPIMSLTGGLSSTTRNDVMWPKSGQGLRESSVMKTLLDERRTSGTQPTTAPVAAEEPRLENVALENFVSIGWKLLDDSELGWY

Target ORF information:

RefSeq Version	NM_001179604.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rgt1p (RGT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179604.3

ORF Insert Sequence:

ATGAACGAGC TGAACACTGT TTCGACTAAC TCCAGTGACT CCACCAAGAA CGGTGGTACA 
TCCAATAGTC CCGACGATAT GGACTCCGCA GCAGCAGCAA GTCACGCAAT AAAGAAAAGA 
ACAAAGGCCT CCCGGGCCTG CGATCAGTGT CGTAAAAAGA AGATTAAATG TGACTACAAA 
GATGAAAAGG GGGTCTGCTC AAACTGCCAA AGGAATGGAG ACCGTTGTAG TTTTGATAGG 
GTTCCTTTGA AAAGGGGACC CTCGAAAGGC TATACGAGGA GCACCAGCCA TCCAAGGACT 
AACGAAATAC AAGACCACAA TAACTCAAGG TCGTATAATA CGTTTGATAA TAGTAACAAT 
ACCCTGAATA ATAATACGGG CAATAGCGGC GACAACGGTA TAAACAGTAA CACAGTTCCA 
AGTACCCCTT CGAGATCTAA TTCAGTTTTA TTGCCCCCAC TGACACAGTA CATTCCCCAG 
GCTGGTGGCA TTCCTCCTAG TTTCCAAAAT CCAGCGATAC AATCAACTAT GCCCGCGGGT 
AATATTGGCC AACAGCAGTT TTGGAAAGTG CCCTACCATG AGTTTCAACA CCAAAGAAAA 
GGATCTATCG ATTCTTTGCA AAGTGATATA TCGGTAAGAA CATTAAATCC CAACGAGCAG 
TTGTCTTATA ACACCGTTCA ACAGTCTCCC ATAACGAATA AACATACTAA TGATTCTGGA 
AATGCAAACG GAAGTGTCAC CGGCTCAGGC AGTGCCTCTG GTAGTGGTGG TTATTGGTCC 
TTTATAAGAA CTTCTGGTTT ACTAGCTCCT ACTGATGATC ATAATGGGGA ACAGACAAGA 
AGATCAAGTT CCATACCTTC TTTGCTACGA AATACTTCAA ATTCTCTTTT ACTAGGTGGC 
CAGCCTCAGC TTCCCCCACC TCAGCAACAA TCGCAGCCAC AAGCACACCA ACAAAAGTTG 
CAACAAGGAC AGAACCTATA CTCGTATTCT CAATTTTCCC AGCAGCAACC GTACAACCCA 
TCGATATCAT CTTTTGGCCA GTTCGCTGCT AATGGTTTTC ATTCTAGACA AGGATCAGTC 
GCCAGCGAGG CTATGTCTCC CAGTGCACCT GCCATGTTTA CTAGCACATC TACAAACCCC 
GTAAATGTTG CACAGCAAAC ACAAAGACCT CAAGGACAGC AGGTACCGCA ATTTTCATCT 
GAGTTGGATG GAAATAAAAG GCGACAATCA GCCCCCGTAT CAGTAACATT ATCCACGGAC 
AGGCTGAATG GTAATGAGAA TAATAATGGT GAAATTAACA ACAATAATGG CAGCAATAAT 
AGCGGTTCTT CTAAGGATAC ATCTCAACAT TCCCAAGAAT CTGTAACTAC ACCAGCGGCT 
TTGGAAGCAT CCAGCCCTGG ATCAACGCCA CAGAGAAGTA CAAAAAAACG CAGAAAAAGT 
TACGTATCTA AGAAGACAAA ACCAAAGAGA GATTCATCTA TATCTATAAC ATCGAAAGAT 
TCTGCTCACC CAATGACCAC TTCATCAACT ATCGCGTATG GACAGATATC CGATGTAGAT 
CTAATAGACA CCTACTATGA GTTCATACAT GTAGGATTTC CGATCATACC TTTAAACAAA 
ACGACCTTGA CCAGTGACTT ATTGTTAGTT AACACACAGC CAATTTCCAA TATACACGAA 
GTCAATTCAT ATGTTATTTT GTGGTTTAGA AATTCATTGG AATTGTTAGT TCGTGTTGCT 
CTGAAACAAA AGCCAGGTGG CAAGTTTTTC GATAATATTG TTGGCGTGGC TTTGTCGCCA 
AGTAATGACA ACAACAAAGC TGGGTTCACT ACAGCCACGG CAAGGGATGA TGCTGAAAAA 
ACAAGACGTG ATTCACATAA TGAAGTACAG GATACTTTGG AAGTGCAAAG CGTTTTTATT 
GCCGCCCTCA ATGAATGTTT CCAAAAAATC GTGGATATTC ATCCCAAATT CAGAGAAAAC 
AACGACCAAA TTTCGCCGAA GATCAAAGTC ATTTATTTAT CTACTTTTAT TCTTTTAAAT 
TACATTTTGG CATTTGTTGG ATACGATAAC TCATTTGTAC TTGGAATGTC GGTGACAATT 
TTTAACGAAT TTAAATTATA CAAACTTCTA TTATTTCCGG AGCCGGATAT AAATGATGTG 
AAGCCTCCAG TTGATGAAGA GGTCAGCACT GGTAATGGGA ATACAAAAAC GTCCGAATTT 
GAAATTGGAT CTGAAAGTGC TGGGCATATG AATCCATCCA ATTCACCAAA TTCCATGGAC 
GAAAACATTA GTCACTATTC AGTGTTGTTT AAAAGATTAT ACGTTTTGCT TTCAGTGTTT 
GATTCTTTAC AAAGCTGTGC ATTCGGTGGT CCCAAGCTAT TAAACATTTC CATCCAAGGT 
TCTACAGAAA GATTTTTTTC TAATGATTTG GGCTCAAAAT GGTGCCTGGA ACAAAGCCAA 
TTGAGACTGA AAAGCGTCTT GCAAAGCTTG AAATTGGGTG AATTGATGAG TGAGCTTACC 
AGGAATAGAA TATCAATGAA CGGCAATAGG AAGCCTGGGT TCGATATTAC GAACTCGTCT 
TCACTCTTAT CGGAATATGT GGAAACTCAA CCTCTATCCG TAGCACAGTT ATTTTGCAAG 
CTATTAATTG GCAAACACAA TTTCATCAAT TGCTTATTAT CATTATACGA TTCAGAAGCA 
GGAGTCTATT CAGATTTGAC ATTGGATTTG AGTTCGAAAA TAGCAGACTC TCTGTGCTCA 
TTGATATCAA TAATTTTGCA AGTATTGACG TTGATATTAA GACTGAATCC TACGAACAGT 
ATTGATTTTA ATTATAGACC ACCGAACCCA CCCGCTAATA ATCCGACAGT GCAAGAGGGC 
CCATCTGCTA TGGGCTCGTC TCCAGTCGCT GGGAACCTTA GCGCTGCACC TCCATCGGAG 
GGGAATCCAG ATTTTTACAA GAAATTACTA GGCCTGAAAC AAGACACTGG CACTATCCTT 
TCAGACCTTT GCCGGGGGAT AATTTCCCCC TTTGCTATTG CTATCCTGCA CGAGGTCTAC 
AACATCACTG AACTGGTCAA GCAGATGCCT ACATCACTCA TTAGCATTAT GATGACGGCA 
ACTACAACGC AGAATACTCA GGACACCAAG AAGTCGCAGG ACCTGGTCAT GAAGCTGTCA 
AACTCGATGA ATGAAGTAGT TCAAATCACC AGCGTGCTGA CAATGATCAA GCCATTCAAG 
ATCTTCGAGC ACGAGCTTAA TAAGCCCATA ATGTCCCTGA CAGGAGGACT TTCGTCCACG 
ACCAGAAACG ACGTCATGTG GCCAAAGTCG GGCCAAGGCC TCCGCGAGTC ATCAGTCATG 
AAGACATTGC TTGATGAACG CCGTACTTCT GGTACACAAC CGACAACGGC GCCAGTAGCC 
GCAGAGGAAC CCAGGCTTGA GAACGTTGCC CTGGAAAATT TCGTTAGTAT CGGCTGGAAG 
CTGTTGGACG ATTCCGAGTT AGGCTGGTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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RGT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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