NUP100 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP100
Entrez Gene ID	853796
Full Name	FG-nucleoporin NUP100
Synonyms	NSP100
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179634.1	NP_012855.1	FG-nucleoporin NUP100

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GCN4 is required for the elevated life spans of nup100-null mutants, similar to other previously described tRNA export and ribosomal mutants.
Title: Nup100 regulates

These data provide evidence that the export of Msn5 is likely mediated by a specific subset of FG-Nups and that the GLFG repeat domain of Nup100 is important for Msn5-mediated nuclear protein export.
Title: A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export.

The following NUP100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02993	NM_001179634.1 Latest version!	Saccharomyces cerevisiae S288C FG-nucleoporin NUP100 (NUP100), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP100 ( NM_001179634.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012855.1 Saccharomyces cerevisiae S288C FG-nucleoporin NUP100 (NUP100), partial mRNA.

Price & Availability↑

CloneID	OSi02993
Clone ID Related Accession (Same CDS sequence)	NM_001179634.1
Accession Version	NM_001179634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	FG-nucleoporin NUP100
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTTGGCA ACAATAGACC AATGTTTGGA GGGAGCAACC TTTCCTTTGG ATCAAATACG 
TCATCCTTCG GTGGGCAACA ATCGCAACAA CCAAACTCTC TTTTTGGAAA TAGCAATAAC 
AACAACAATT CCACCAGTAA CAATGCCCAA TCAGGATTTG GTGGATTCAC TTCTGCCGCT 
GGCAGTAATA GTAACAGTTT ATTTGGAAAT AACAATACTC AAAATAATGG GGCATTTGGC 
CAGTCAATGG GTGCCACCCA AAACTCACCA TTTGGGTCGT TAAACTCCTC AAATGCCAGT 
AATGGGAACA CTTTTGGAGG ATCCAGTTCA ATGGGTTCTT TTGGTGGTAA TACCAATAAT 
GCATTTAATA ATAACAGTAA CAGTACCAAT TCCCCGTTTG GTTTCAATAA ACCGAATACA 
GGAGGGACCT TGTTTGGTTC GCAGAATAAT AATAGTGCAG GTACTTCTAG TTTATTTGGC 
GGCCAAAGTA CAAGTACCAC TGGCACATTC GGAAACACCG GAAGTAGTTT CGGAACAGGC 
TTAAATGGCA ACGGCTCTAA CATATTTGGG GCAGGGAACA ACTCACAAAG CAATACTACC 
GGCAGCTTGT TTGGCAACCA ACAATCTTCA GCGTTTGGGA CAAATAATCA ACAAGGAAGT 
CTATTTGGAC AGCAATCTCA AAACACCAAT AATGCGTTTG GTAATCAAAA TCAGCTGGGG 
GGCAGTTCCT TCGGATCAAA ACCAGTTGGT TCAGGGTCGC TGTTTGGCCA GAGCAACAAC 
ACTTTAGGTA ATACAACCAA TAATAGAAAT GGATTGTTTG GTCAAATGAA TTCCAGTAAC 
CAAGGCAGTT CTAACAGTGG ATTGTTTGGA CAAAACTCGA TGAACAGCAG CACTCAAGGG 
GTATTTGGAC AAAACAATAA TCAAATGCAA ATTAATGGCA ATAACAACAA CAGTTTATTT 
GGAAAGGCAA ACACCTTTTC AAATTCCGCA TCAGGAGGTT TATTTGGCCA AAATAATCAA 
CAGCAAGGGT CCGGGCTGTT TGGTCAAAAT TCTCAGACAA GCGGTAGTAG TGGACTTTTT 
GGGCAGAATA ATCAGAAACA GCCCAATACT TTTACCCAAT CTAATACAGG AATAGGTCTC 
TTTGGACAGA ACAACAACCA GCAACAACAA TCTACAGGTT TGTTTGGAGC CAAACCAGCA 
GGTACCACAG GATCTCTTTT CGGTGGTAAT TCATCAACCC AGCCTAACTC TCTTTTCGGT 
ACTACCAATG TACCTACCTC TAATACACAG TCACAACAAG GAAATAGTCT TTTTGGTGCC 
ACGAAGCTGA CCAACATGCC CTTTGGAGGA AATCCCACTG CAAACCAGTC AGGGAGTGGA 
AACAGTCTAT TCGGAACTAA ACCTGCCTCC ACTACCGGCT CATTATTTGG AAACAACACA 
GCTTCTACGA CAGTACCTTC CACGAATGGA TTGTTTGGTA ACAACGCTAA CAATTCAACT 
AGCACTACGA ATACGGGACT TTTCGGTGCA AAGCCTGATT CTCAGTCCAA ACCTGCATTA 
GGGGGAGGGT TATTCGGCAA TTCAAACTCG AATTCTTCCA CAATCGGCCA AAACAAACCA 
GTTTTTGGAG GCACAACCCA AAATACAGGA CTCTTTGGCG CCACCGGCAC GAACTCTTCA 
GCAGTTGGTT CAACTGGTAA ACTTTTTGGC CAGAATAATA ATACGCTTAA TGTTGGTACA 
CAAAATGTAC CACCTGTGAA CAATACCACC CAAAACGCCC TTTTGGGTAC AACGGCAGTT 
CCTTCCCTAC AACAAGCCCC AGTAACTAAT GAACAGCTTT TTTCCAAAAT ATCAATCCCT 
AACTCTATTA CAAATCCAGT TAAAGCAACA ACTTCAAAAG TGAACGCAGA TATGAAAAGA 
AATAGTAGCC TCACGTCTGC CTATAGACTT GCCCCAAAGC CGTTATTTGC TCCCTCTTCG 
AATGGCGATG CTAAATTTCA AAAGTGGGGC AAGACACTGG AAAGAAGTGA TAGAGGAAGC 
AGTACCAGCA ATTCTATTAC GGACCCAGAA TCAAGCTATC TAAATTCAAA CGACTTGTTG 
TTTGATCCAG ATAGAAGATA TTTGAAACAT CTGGTGATTA AAAATAATAA GAACTTAAAT 
GTCATTAACC ATAATGATGA TGAAGCAAGC AAAGTTAAAT TAGTGACGTT TACAACAGAA 
TCAGCTTCAA AAGATGACCA AGCCTCATCA AGCATTGCTG CTTCAAAATT AACTGAAAAA 
GCACATTCTC CTCAGACTGA CCTAAAAGAT GATCATGATG AAAGCACTCC TGATCCTCAA 
TCGAAATCTC CAAATGGTTC CACCTCTATA CCAATGATTG AGAATGAAAA GATTAGCAGC 
AAAGTTCCCG GCCTATTGAG CAACGACGTT ACCTTTTTCA AGAATAACTA CTACATTTCA 
CCTTCCATAG AAACGCTTGG CAATAAGTCA TTAATTGAAC TTCGTAAAAT AAACAACCTA 
GTCATTGGTC ACAGAAATTA TGGTAAAGTC GAGTTTCTGG AGCCCGTTGA TTTGTTGAAT 
ACTCCTTTGG ATACTTTATG CGGGGATCTT GTCACCTTTG GACCAAAATC ATGTTCAATA 
TATGAAAACT GTTCCATAAA GCCAGAAAAG GGCGAAGGCA TTAATGTACG TTGTAGAGTG 
ACTTTATATT CCTGTTTTCC TATTGACAAA GAAACAAGGA AACCTATAAA GAATATAACA 
CATCCTCTAC TGAAAAGAAG TATAGCCAAA CTAAAAGAAA ACCCAGTGTA CAAGTTTGAA 
AGCTATGACC CCGTAACAGG CACCTATAGT TACACCATAG ATCACCCAGT TTTAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012855.1
CDS	1..2880
Translation	MFGNNRPMFGGSNLSFGSNTSSFGGQQSQQPNSLFGNSNNNNNSTSNNAQSGFGGFTSAAGSNSNSLFGNNNTQNNGAFGQSMGATQNSPFGSLNSSNASNGNTFGGSSSMGSFGGNTNNAFNNNSNSTNSPFGFNKPNTGGTLFGSQNNNSAGTSSLFGGQSTSTTGTFGNTGSSFGTGLNGNGSNIFGAGNNSQSNTTGSLFGNQQSSAFGTNNQQGSLFGQQSQNTNNAFGNQNQLGGSSFGSKPVGSGSLFGQSNNTLGNTTNNRNGLFGQMNSSNQGSSNSGLFGQNSMNSSTQGVFGQNNNQMQINGNNNNSLFGKANTFSNSASGGLFGQNNQQQGSGLFGQNSQTSGSSGLFGQNNQKQPNTFTQSNTGIGLFGQNNNQQQQSTGLFGAKPAGTTGSLFGGNSSTQPNSLFGTTNVPTSNTQSQQGNSLFGATKLTNMPFGGNPTANQSGSGNSLFGTKPASTTGSLFGNNTASTTVPSTNGLFGNNANNSTSTTNTGLFGAKPDSQSKPALGGGLFGNSNSNSSTIGQNKPVFGGTTQNTGLFGATGTNSSAVGSTGKLFGQNNNTLNVGTQNVPPVNNTTQNALLGTTAVPSLQQAPVTNEQLFSKISIPNSITNPVKATTSKVNADMKRNSSLTSAYRLAPKPLFAPSSNGDAKFQKWGKTLERSDRGSSTSNSITDPESSYLNSNDLLFDPDRRYLKHLVIKNNKNLNVINHNDDEASKVKLVTFTTESASKDDQASSSIAASKLTEKAHSPQTDLKDDHDESTPDPQSKSPNGSTSIPMIENEKISSKVPGLLSNDVTFFKNNYYISPSIETLGNKSLIELRKINNLVIGHRNYGKVEFLEPVDLLNTPLDTLCGDLVTFGPKSCSIYENCSIKPEKGEGINVRCRVTLYSCFPIDKETRKPIKNITHPLLKRSIAKLKENPVYKFESYDPVTGTYSYTIDHPVLT

Target ORF information:

RefSeq Version	NM_001179634.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C FG-nucleoporin NUP100 (NUP100), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179634.1

ORF Insert Sequence:

ATGTTTGGCA ACAATAGACC AATGTTTGGA GGGAGCAACC TTTCCTTTGG ATCAAATACG 
TCATCCTTCG GTGGGCAACA ATCGCAACAA CCAAACTCTC TTTTTGGAAA TAGCAATAAC 
AACAACAATT CCACCAGTAA CAATGCCCAA TCAGGATTTG GTGGATTCAC TTCTGCCGCT 
GGCAGTAATA GTAACAGTTT ATTTGGAAAT AACAATACTC AAAATAATGG GGCATTTGGC 
CAGTCAATGG GTGCCACCCA AAACTCACCA TTTGGGTCGT TAAACTCCTC AAATGCCAGT 
AATGGGAACA CTTTTGGAGG ATCCAGTTCA ATGGGTTCTT TTGGTGGTAA TACCAATAAT 
GCATTTAATA ATAACAGTAA CAGTACCAAT TCCCCGTTTG GTTTCAATAA ACCGAATACA 
GGAGGGACCT TGTTTGGTTC GCAGAATAAT AATAGTGCAG GTACTTCTAG TTTATTTGGC 
GGCCAAAGTA CAAGTACCAC TGGCACATTC GGAAACACCG GAAGTAGTTT CGGAACAGGC 
TTAAATGGCA ACGGCTCTAA CATATTTGGG GCAGGGAACA ACTCACAAAG CAATACTACC 
GGCAGCTTGT TTGGCAACCA ACAATCTTCA GCGTTTGGGA CAAATAATCA ACAAGGAAGT 
CTATTTGGAC AGCAATCTCA AAACACCAAT AATGCGTTTG GTAATCAAAA TCAGCTGGGG 
GGCAGTTCCT TCGGATCAAA ACCAGTTGGT TCAGGGTCGC TGTTTGGCCA GAGCAACAAC 
ACTTTAGGTA ATACAACCAA TAATAGAAAT GGATTGTTTG GTCAAATGAA TTCCAGTAAC 
CAAGGCAGTT CTAACAGTGG ATTGTTTGGA CAAAACTCGA TGAACAGCAG CACTCAAGGG 
GTATTTGGAC AAAACAATAA TCAAATGCAA ATTAATGGCA ATAACAACAA CAGTTTATTT 
GGAAAGGCAA ACACCTTTTC AAATTCCGCA TCAGGAGGTT TATTTGGCCA AAATAATCAA 
CAGCAAGGGT CCGGGCTGTT TGGTCAAAAT TCTCAGACAA GCGGTAGTAG TGGACTTTTT 
GGGCAGAATA ATCAGAAACA GCCCAATACT TTTACCCAAT CTAATACAGG AATAGGTCTC 
TTTGGACAGA ACAACAACCA GCAACAACAA TCTACAGGTT TGTTTGGAGC CAAACCAGCA 
GGTACCACAG GATCTCTTTT CGGTGGTAAT TCATCAACCC AGCCTAACTC TCTTTTCGGT 
ACTACCAATG TACCTACCTC TAATACACAG TCACAACAAG GAAATAGTCT TTTTGGTGCC 
ACGAAGCTGA CCAACATGCC CTTTGGAGGA AATCCCACTG CAAACCAGTC AGGGAGTGGA 
AACAGTCTAT TCGGAACTAA ACCTGCCTCC ACTACCGGCT CATTATTTGG AAACAACACA 
GCTTCTACGA CAGTACCTTC CACGAATGGA TTGTTTGGTA ACAACGCTAA CAATTCAACT 
AGCACTACGA ATACGGGACT TTTCGGTGCA AAGCCTGATT CTCAGTCCAA ACCTGCATTA 
GGGGGAGGGT TATTCGGCAA TTCAAACTCG AATTCTTCCA CAATCGGCCA AAACAAACCA 
GTTTTTGGAG GCACAACCCA AAATACAGGA CTCTTTGGCG CCACCGGCAC GAACTCTTCA 
GCAGTTGGTT CAACTGGTAA ACTTTTTGGC CAGAATAATA ATACGCTTAA TGTTGGTACA 
CAAAATGTAC CACCTGTGAA CAATACCACC CAAAACGCCC TTTTGGGTAC AACGGCAGTT 
CCTTCCCTAC AACAAGCCCC AGTAACTAAT GAACAGCTTT TTTCCAAAAT ATCAATCCCT 
AACTCTATTA CAAATCCAGT TAAAGCAACA ACTTCAAAAG TGAACGCAGA TATGAAAAGA 
AATAGTAGCC TCACGTCTGC CTATAGACTT GCCCCAAAGC CGTTATTTGC TCCCTCTTCG 
AATGGCGATG CTAAATTTCA AAAGTGGGGC AAGACACTGG AAAGAAGTGA TAGAGGAAGC 
AGTACCAGCA ATTCTATTAC GGACCCAGAA TCAAGCTATC TAAATTCAAA CGACTTGTTG 
TTTGATCCAG ATAGAAGATA TTTGAAACAT CTGGTGATTA AAAATAATAA GAACTTAAAT 
GTCATTAACC ATAATGATGA TGAAGCAAGC AAAGTTAAAT TAGTGACGTT TACAACAGAA 
TCAGCTTCAA AAGATGACCA AGCCTCATCA AGCATTGCTG CTTCAAAATT AACTGAAAAA 
GCACATTCTC CTCAGACTGA CCTAAAAGAT GATCATGATG AAAGCACTCC TGATCCTCAA 
TCGAAATCTC CAAATGGTTC CACCTCTATA CCAATGATTG AGAATGAAAA GATTAGCAGC 
AAAGTTCCCG GCCTATTGAG CAACGACGTT ACCTTTTTCA AGAATAACTA CTACATTTCA 
CCTTCCATAG AAACGCTTGG CAATAAGTCA TTAATTGAAC TTCGTAAAAT AAACAACCTA 
GTCATTGGTC ACAGAAATTA TGGTAAAGTC GAGTTTCTGG AGCCCGTTGA TTTGTTGAAT 
ACTCCTTTGG ATACTTTATG CGGGGATCTT GTCACCTTTG GACCAAAATC ATGTTCAATA 
TATGAAAACT GTTCCATAAA GCCAGAAAAG GGCGAAGGCA TTAATGTACG TTGTAGAGTG 
ACTTTATATT CCTGTTTTCC TATTGACAAA GAAACAAGGA AACCTATAAA GAATATAACA 
CATCCTCTAC TGAAAAGAAG TATAGCCAAA CTAAAAGAAA ACCCAGTGTA CAAGTTTGAA 
AGCTATGACC CCGTAACAGG CACCTATAGT TACACCATAG ATCACCCAGT TTTAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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NUP100 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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