LHS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	LHS1
Entrez Gene ID	853789
Full Name	Hsp70 family chaperone LHS1
Synonyms	CER1,SSI1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179639.1	NP_012850.1	Hsp70 family chaperone LHS1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADR128C	NP_984224.1
Saccharomyces cerevisiae (baker's yeast)	LHS1	NP_012850.1
Kluyveromyces lactis	KLLA0F26455g	XP_456259.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data indicate that the Lhs1 chaperone selectively recognizes an immature form of alphaENaC, one which has failed to correctly assemble with the other channel subunits via its transmembrane domain.
Title: Interactions between intersubunit transmembrane domains regulate the chaperone-dependent degradation of an oligomeric membrane protein.

Interactions between Kar2p and its nucleotide exchange factors Sil1p and Lhs1p are mechanistically distinct
Title: Interactions between Kar2p and its nucleotide exchange factors Sil1p and Lhs1p are mechanistically distinct.

compare Grp170 Lhs1 with Hsp110 Sse1 and find that residues important for Sse1 nucleotide exchange factor (NEF) activity are conserved in Lhs1 and that mutations in these residues in Lhs1 compromise NEF activity
Title: The endoplasmic reticulum Grp170 acts as a nucleotide exchange factor of Hsp70 via a mechanism similar to that of the cytosolic Hsp110.

The following LHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02988	NM_001179639.1 Latest version!	Saccharomyces cerevisiae S288C Hsp70 family chaperone LHS1 (LHS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LHS1 ( NM_001179639.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012850.1 Saccharomyces cerevisiae S288C Hsp70 family chaperone LHS1 (LHS1), partial mRNA.

Price & Availability↑

CloneID	OSi02988
Clone ID Related Accession (Same CDS sequence)	NM_001179639.1
Accession Version	NM_001179639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Hsp70 family chaperone LHS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCGAAACG TTTTAAGGCT TTTATTTTTA ACAGCTTTTG TTGCTATAGG GTCTTTAGCA 
GCCGTTTTAG GTGTTGATTA CGGTCAGCAA AATATCAAGG CCATTGTGGT TTCTCCGCAA 
GCCCCATTAG AACTTGTGCT CACACCAGAG GCAAAACGGA AGGAGATATC TGGTCTTTCG 
ATAAAAAGAT TACCAGGTTA TGGAAAGGAT GATCCGAATG GGATTGAAAG AATCTACGGT 
TCCGCTGTTG GCAGTTTAGC AACAAGGTTT CCCCAAAACA CATTGTTGCA TTTGAAACCG 
CTACTTGGGA AATCACTAGA AGATGAAACC ACTGTAACTT TGTATTCAAA ACAACACCCC 
GGTTTAGAAA TGGTATCAAC AAATAGAAGT ACCATAGCCT TTTTAGTTGA TAATGTGGAA 
TATCCATTGG AAGAGTTAGT GGCAATGAAT GTCCAAGAGA TTGCCAATAG AGCCAATTCA 
CTGTTGAAGG ATAGAGATGC AAGAACTGAG GACTTTGTAA ACAAGATGAG TTTTACAATT 
CCTGACTTTT TTGACCAACA TCAAAGGAAA GCACTTTTAG ATGCCAGTTC AATAACCACA 
GGAATCGAAG AGACATATCT GGTTAGTGAA GGGATGTCTG TTGCAGTTAA CTTTGTATTA 
AAGCAGCGCC AATTTCCACC AGGTGAACAG CAGCATTATA TCGTATATGA CATGGGGAGC 
GGTTCTATTA AGGCCTCAAT GTTCTCTATA TTGCAGCCGG AGGACACTAC TCAGCCCGTT 
ACAATAGAAT TTGAAGGATA TGGGTATAAT CCACATCTAG GTGGTGCAAA GTTTACAATG 
GATATTGGCA GTTTGATAGA GAATAAGTTT TTGGAAACAC ACCCAGCCAT AAGAACTGAT 
GAATTGCACG CTAATCCCAA GGCCTTAGCA AAAATCAACC AAGCAGCAGA GAAGGCAAAG 
TTAATTTTAA GCGCCAATTC TGAGGCAAGT ATTAACATAG AATCACTGAT CAACGATATT 
GATTTCCGTA CTTCTATAAC TAGACAGGAA TTCGAAGAAT TTATTGCAGA CTCGTTATTG 
GACATTGTCA AACCCATAAA TGACGCTGTT ACAAAACAAT TCGGTGGCTA TGGAACAAAT 
TTACCTGAGA TAAATGGGGT CATTTTGGCG GGAGGCTCTT CCCGAATTCC CATTGTGCAG 
GATCAATTAA TCAAACTCGT ATCCGAAGAA AAAGTGTTGA GAAATGTCAA TGCTGATGAA 
TCAGCTGTGA ATGGTGTTGT TATGAGAGGG ATCAAGTTAT CTAATTCGTT TAAGACCAAG 
CCGTTAAATG TTGTTGACCG TTCTGTAAAT ACTTATTCAT TCAAATTATC AAACGAATCT 
GAACTGTATG ATGTGTTCAC GCGCGGAAGT GCTTATCCAA ACAAAACATC TATTTTGACA 
AACACGACTG ATTCGATTCC TAATAATTTT ACCATTGACT TATTTGAGAA TGGTAAATTG 
TTCGAAACTA TCACAGTTAA TTCAGGAGCT ATAAAGAATT CATATTCCTC TGATAAGTGC 
TCGTCAGGAG TTGCGTATAA CATTACTTTC GACTTGTCCA GTGATAGATT ATTCTCTATT 
CAAGAGGTTA ACTGCATTTG TCAGAGCGAA AATGACATAG GTAACTCCAA GCAAATTAAG 
AACAAAGGCA GCCGTTTGGC TTTTACTTCT GAGGATGTTG AGATCAAAAG GCTTTCTCCT 
TCAGAACGTT CGCGTTTGCA TGAGCATATC AAGTTGCTCG ATAAACAGGA TAAGGAAAGA 
TTTCAATTCC AAGAAAATTT AAACGTTCTT GAAAGTAACT TGTATGATGC TAGAAACCTG 
CTAATGGATG ATGAAGTTAT GCAAAATGGA CCAAAATCCC AAGTAGAAGA GTTATCGGAG 
ATGGTTAAAG TATATTTGGA TTGGCTCGAA GATGCATCCT TTGATACTGA CCCTGAGGAT 
ATAGTTAGCA GAATTAGAGA AATTGGAATA TTAAAAAAGA AAATAGAACT TTACATGGAT 
TCTGCAAAGG AACCTTTGAA CTCTCAACAA TTTAAAGGAA TGCTTGAAGA AGGCCATAAG 
TTACTTCAGG CTATAGAAAC CCATAAGAAT ACCGTTGAAG AATTTTTGAG TCAATTTGAA 
ACCGAGTTTG CGGATACCAT AGATAATGTT AGAGAAGAAT TTAAAAAGAT TAAGCAACCA 
GCGTATGTGT CGAAGGCGTT ATCTACATGG GAGGAAACCT TAACCTCTTT TAAAAATTCC 
ATTAGCGAAA TAGAGAAGTT CCTGGCAAAA AACCTATTTG GCGAAGACCT TCGTGAACAT 
TTATTTGAAA TCAAATTACA ATTTGATATG TATCGTACGA AACTAGAGGA AAAACTGCGT 
TTAATAAAAA GCGGTGATGA AAGTCGCTTA AATGAAATAA AGAAGTTACA TTTAAGAAAC 
TTCCGCCTAC AAAAGAGAAA GGAGGAAAAG TTGAAAAGAA AGCTTGAACA GGAAAAAAGC 
AGAAACAACA ATGAAACAGA ATCGACAGTA ATCAACTCGG CTGACGATAA AACTACTATT 
GTCAATGACA AGACCACCGA GTCGAATCCA AGTTCTGAGG AAGACATTTT GCATGATGAA 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012850.1
CDS	1..2646
Translation	MRNVLRLLFLTAFVAIGSLAAVLGVDYGQQNIKAIVVSPQAPLELVLTPEAKRKEISGLSIKRLPGYGKDDPNGIERIYGSAVGSLATRFPQNTLLHLKPLLGKSLEDETTVTLYSKQHPGLEMVSTNRSTIAFLVDNVEYPLEELVAMNVQEIANRANSLLKDRDARTEDFVNKMSFTIPDFFDQHQRKALLDASSITTGIEETYLVSEGMSVAVNFVLKQRQFPPGEQQHYIVYDMGSGSIKASMFSILQPEDTTQPVTIEFEGYGYNPHLGGAKFTMDIGSLIENKFLETHPAIRTDELHANPKALAKINQAAEKAKLILSANSEASINIESLINDIDFRTSITRQEFEEFIADSLLDIVKPINDAVTKQFGGYGTNLPEINGVILAGGSSRIPIVQDQLIKLVSEEKVLRNVNADESAVNGVVMRGIKLSNSFKTKPLNVVDRSVNTYSFKLSNESELYDVFTRGSAYPNKTSILTNTTDSIPNNFTIDLFENGKLFETITVNSGAIKNSYSSDKCSSGVAYNITFDLSSDRLFSIQEVNCICQSENDIGNSKQIKNKGSRLAFTSEDVEIKRLSPSERSRLHEHIKLLDKQDKERFQFQENLNVLESNLYDARNLLMDDEVMQNGPKSQVEELSEMVKVYLDWLEDASFDTDPEDIVSRIREIGILKKKIELYMDSAKEPLNSQQFKGMLEEGHKLLQAIETHKNTVEEFLSQFETEFADTIDNVREEFKKIKQPAYVSKALSTWEETLTSFKNSISEIEKFLAKNLFGEDLREHLFEIKLQFDMYRTKLEEKLRLIKSGDESRLNEIKKLHLRNFRLQKRKEEKLKRKLEQEKSRNNNETESTVINSADDKTTIVNDKTTESNPSSEEDILHDEL

Target ORF information:

RefSeq Version	NM_001179639.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Hsp70 family chaperone LHS1 (LHS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179639.1

ORF Insert Sequence:

ATGCGAAACG TTTTAAGGCT TTTATTTTTA ACAGCTTTTG TTGCTATAGG GTCTTTAGCA 
GCCGTTTTAG GTGTTGATTA CGGTCAGCAA AATATCAAGG CCATTGTGGT TTCTCCGCAA 
GCCCCATTAG AACTTGTGCT CACACCAGAG GCAAAACGGA AGGAGATATC TGGTCTTTCG 
ATAAAAAGAT TACCAGGTTA TGGAAAGGAT GATCCGAATG GGATTGAAAG AATCTACGGT 
TCCGCTGTTG GCAGTTTAGC AACAAGGTTT CCCCAAAACA CATTGTTGCA TTTGAAACCG 
CTACTTGGGA AATCACTAGA AGATGAAACC ACTGTAACTT TGTATTCAAA ACAACACCCC 
GGTTTAGAAA TGGTATCAAC AAATAGAAGT ACCATAGCCT TTTTAGTTGA TAATGTGGAA 
TATCCATTGG AAGAGTTAGT GGCAATGAAT GTCCAAGAGA TTGCCAATAG AGCCAATTCA 
CTGTTGAAGG ATAGAGATGC AAGAACTGAG GACTTTGTAA ACAAGATGAG TTTTACAATT 
CCTGACTTTT TTGACCAACA TCAAAGGAAA GCACTTTTAG ATGCCAGTTC AATAACCACA 
GGAATCGAAG AGACATATCT GGTTAGTGAA GGGATGTCTG TTGCAGTTAA CTTTGTATTA 
AAGCAGCGCC AATTTCCACC AGGTGAACAG CAGCATTATA TCGTATATGA CATGGGGAGC 
GGTTCTATTA AGGCCTCAAT GTTCTCTATA TTGCAGCCGG AGGACACTAC TCAGCCCGTT 
ACAATAGAAT TTGAAGGATA TGGGTATAAT CCACATCTAG GTGGTGCAAA GTTTACAATG 
GATATTGGCA GTTTGATAGA GAATAAGTTT TTGGAAACAC ACCCAGCCAT AAGAACTGAT 
GAATTGCACG CTAATCCCAA GGCCTTAGCA AAAATCAACC AAGCAGCAGA GAAGGCAAAG 
TTAATTTTAA GCGCCAATTC TGAGGCAAGT ATTAACATAG AATCACTGAT CAACGATATT 
GATTTCCGTA CTTCTATAAC TAGACAGGAA TTCGAAGAAT TTATTGCAGA CTCGTTATTG 
GACATTGTCA AACCCATAAA TGACGCTGTT ACAAAACAAT TCGGTGGCTA TGGAACAAAT 
TTACCTGAGA TAAATGGGGT CATTTTGGCG GGAGGCTCTT CCCGAATTCC CATTGTGCAG 
GATCAATTAA TCAAACTCGT ATCCGAAGAA AAAGTGTTGA GAAATGTCAA TGCTGATGAA 
TCAGCTGTGA ATGGTGTTGT TATGAGAGGG ATCAAGTTAT CTAATTCGTT TAAGACCAAG 
CCGTTAAATG TTGTTGACCG TTCTGTAAAT ACTTATTCAT TCAAATTATC AAACGAATCT 
GAACTGTATG ATGTGTTCAC GCGCGGAAGT GCTTATCCAA ACAAAACATC TATTTTGACA 
AACACGACTG ATTCGATTCC TAATAATTTT ACCATTGACT TATTTGAGAA TGGTAAATTG 
TTCGAAACTA TCACAGTTAA TTCAGGAGCT ATAAAGAATT CATATTCCTC TGATAAGTGC 
TCGTCAGGAG TTGCGTATAA CATTACTTTC GACTTGTCCA GTGATAGATT ATTCTCTATT 
CAAGAGGTTA ACTGCATTTG TCAGAGCGAA AATGACATAG GTAACTCCAA GCAAATTAAG 
AACAAAGGCA GCCGTTTGGC TTTTACTTCT GAGGATGTTG AGATCAAAAG GCTTTCTCCT 
TCAGAACGTT CGCGTTTGCA TGAGCATATC AAGTTGCTCG ATAAACAGGA TAAGGAAAGA 
TTTCAATTCC AAGAAAATTT AAACGTTCTT GAAAGTAACT TGTATGATGC TAGAAACCTG 
CTAATGGATG ATGAAGTTAT GCAAAATGGA CCAAAATCCC AAGTAGAAGA GTTATCGGAG 
ATGGTTAAAG TATATTTGGA TTGGCTCGAA GATGCATCCT TTGATACTGA CCCTGAGGAT 
ATAGTTAGCA GAATTAGAGA AATTGGAATA TTAAAAAAGA AAATAGAACT TTACATGGAT 
TCTGCAAAGG AACCTTTGAA CTCTCAACAA TTTAAAGGAA TGCTTGAAGA AGGCCATAAG 
TTACTTCAGG CTATAGAAAC CCATAAGAAT ACCGTTGAAG AATTTTTGAG TCAATTTGAA 
ACCGAGTTTG CGGATACCAT AGATAATGTT AGAGAAGAAT TTAAAAAGAT TAAGCAACCA 
GCGTATGTGT CGAAGGCGTT ATCTACATGG GAGGAAACCT TAACCTCTTT TAAAAATTCC 
ATTAGCGAAA TAGAGAAGTT CCTGGCAAAA AACCTATTTG GCGAAGACCT TCGTGAACAT 
TTATTTGAAA TCAAATTACA ATTTGATATG TATCGTACGA AACTAGAGGA AAAACTGCGT 
TTAATAAAAA GCGGTGATGA AAGTCGCTTA AATGAAATAA AGAAGTTACA TTTAAGAAAC 
TTCCGCCTAC AAAAGAGAAA GGAGGAAAAG TTGAAAAGAA AGCTTGAACA GGAAAAAAGC 
AGAAACAACA ATGAAACAGA ATCGACAGTA ATCAACTCGG CTGACGATAA AACTACTATT 
GTCAATGACA AGACCACCGA GTCGAATCCA AGTTCTGAGG AAGACATTTT GCATGATGAA 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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LHS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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