HSL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HSL1
Entrez Gene ID	853760
Full Name	protein kinase HSL1
Synonyms	ELM2,NIK1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179667.2	NP_012821.2	protein kinase HSL1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ABL034W	NP_982913.1
Saccharomyces cerevisiae (baker's yeast)	HSL1	NP_012821.2
Kluyveromyces lactis	KLLA0F13552g	XP_455690.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Septin collar assembly is required for recruitment of Hsl1 to the bud neck.
Title: Coordinate action of distinct sequence elements localizes checkpoint kinase Hsl1 to the septin collar at the bud neck in Saccharomyces cerevisiae.

Hsl1p interaction with Hsl7p is not needed for degradation of septin-Swe1p fusion protein.
Title: Roles of Hsl1p and Hsl7p in Swe1p degradation: beyond septin tethering.

the "on-off" states of Nik1 affect fludioxonil sensitivity in the experimental host of S. cerevisiae
Title: Fungal fludioxonil sensitivity is diminished by a constitutively active form of the group III histidine kinase.

Cells expressing stabilized Hsl1 were sensitive to caffeine and failed to activate the Slt2 pathway.
Title: APC/CCdh1 specific degradation of Hsl1 and Clb2 is required for proper stress responses of S. cerevisiae.

Hsl1p responds to alterations in septin organization, which themselves occur in response to the local geometry of the cell cortex.
Title: Molecular dissection of the checkpoint kinase Hsl1p.

The following HSL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02962	NM_001179667.2 Latest version!	Saccharomyces cerevisiae S288C protein kinase HSL1 (HSL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSL1 ( NM_001179667.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012821.2 Saccharomyces cerevisiae S288C protein kinase HSL1 (HSL1), partial mRNA.

Price & Availability↑

CloneID	OSi02962
Clone ID Related Accession (Same CDS sequence)	NM_001179667.2
Accession Version	NM_001179667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase HSL1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Apr 26, 2011 this sequence version replaced NM_001179667.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGGTC ACGTTTCAAA AACGAGCCAC GTACCCAAAG GTCGCCCGTC TTCATTGGCC 
AAGAAGGCAG CTAAAAGGGC CATGGCCAAA GTAAATTCGA ATCCAAAAAG GGCGTCGGGG 
CATTTGGAGC GTGTTGTACA ATCTGTGAAC GACGCTACCA AAAGATTATC TCAGCCCGAC 
TCTACCGTAA GTGTTGCTAC AAAATCATCA AAGAGAAAAT CCAGAGACAC GGTGGGTCCT 
TGGAAACTTG GCAAAACATT GGGGAAGGGA TCTTCTGGTA GGGTTCGCTT GGCTAAAAAT 
ATGGAAACAG GACAATTAGC TGCCATTAAA ATTGTCCCCA AGAAGAAAGC CTTTGTTCAT 
TGTTCTAATA ATGGAACTGT TCCTAATTCT TACTCTTCTT CCATGGTCAC TTCCAATGTA 
TCTTCTCCAT CAATAGCATC TAGAGAACAT AGTAACCATT CTCAAACGAA CCCATATGGC 
ATAGAGAGAG AAATAGTAAT CATGAAATTG ATATCTCACA CAAACGTTAT GGCATTGTTT 
GAAGTTTGGG AGAACAAATC AGAGCTGTAC CTAGTTTTGG AATATGTTGA TGGTGGCGAA 
TTATTCGATT ATCTGGTCTC GAAGGGAAAG CTACCTGAAA GGGAGGCTAT CCATTACTTC 
AAGCAGATAG TTGAAGGTGT TTCCTATTGT CATTCTTTTA ATATATGCCA TCGTGATCTG 
AAGCCTGAAA ATTTATTATT AGATAAAAAG AATAGGAGAA TCAAAATTGC TGATTTCGGC 
ATGGCAGCAT TAGAGCTTCC TAATAAACTT TTAAAAACTT CATGTGGCTC TCCACACTAT 
GCGTCTCCTG AAATTGTTAT GGGTAGACCA TATCATGGCG GTCCTAGCGA TGTCTGGTCC 
TGCGGTATCG TTTTATTTGC ATTACTCACC GGTCATTTAC CATTTAATGA TGACAACATC 
AAAAAGCTGT TGTTGAAAGT TCAATCGGGC AAATATCAAA TGCCCTCAAA TTTATCTTCT 
GAGGCTCGTG ATTTGATATC AAAAATATTG GTAATTGATC CAGAAAAAAG AATTACCACT 
CAAGAGATAT TGAAGCATCC TCTGATCAAA AAATATGATG ATCTCCCAGT TAATAAAGTG 
CTGAGGAAAA TGAGAAAGGA TAACATGGCG AGGGGAAAAT CTAACTCGGA TTTGCATTTG 
CTAAATAATG TCTCGCCCTC TATTGTAACT TTACATTCAA AAGGGGAAAT TGATGAATCC 
ATCTTGAGGA GTTTGCAAAT TTTGTGGCAT GGAGTTTCTC GTGAACTAAT CACTGCCAAG 
TTGCTTCAAA AGCCAATGTC GGAGGAAAAA CTATTCTACT CACTGTTATT ACAATATAAA 
CAACGTCACT CAATATCATT GTCCTCGTCA AGCGAAAACA AGAAATCAGC AACAGAAAGT 
AGCGTAAATG AACCTAGGAT TGAATACGCA TCCAAAACTG CCAATAATAC AGGCCTAAGG 
AGCGAGAACA ATGATGTGAA GACTTTACAT TCTCTAGAAA TTCATTCAGA AGATACTTCA 
ACAGTGAATC AGAACAACGC AATTACAGGG GTTAATACAG AAATTAATGC CCCTGTATTG 
GCACAAAAAT CGCAATTTAG TATCAATACG CTCAGTCAAC CTGAAAGTGA TAAGGCTGAA 
GCAGAAGCTG TAACATTACC ACCCGCTATT CCCATATTCA ATGCTTCTTC ATCAAGGATT 
TTCCGTAACT CATACACCTC TATTTCATCA CGTTCGAGAA GATCGCTACG CTTATCGAAT 
TCCAGATTAT CATTGTCCGC TTCAACTTCT AGAGAAACTG TGCATGATAA TGAGATGCCT 
CTACCTCAAC TGCCGAAGTC GCCTTCAAGA TATTCATTGT CAAGAAGGGC AATCCACGCC 
TCCCCATCTA CTAAATCAAT ACATAAATCA CTTTCGAGGA AGAATATAGC TGCAACTGTT 
GCCGCTAGAA GAACACTACA AAACTCGGCT TCAAAGAGAT CCTTATACTC ATTACAGTCA 
ATTTCAAAAC GTTCCTTGAA CCTGAATGAT TTATTAGTCT TTGACGATCC TCTCCCTAGT 
AAGAAACCAG CATCCGAAAA TGTGAATAAA TCAGAACCCC ATTCGTTGGA ATCAGACTCT 
GATTTTGAGA TCTTATGCGA TCAGATCTTA TTTGGAAACG CCTTAGATAG GATACTCGAA 
GAGGAAGAGG ACAATGAAAA AGAACGCGAC ACCCAAAGAC AGAGGCAAAA CGATACAAAA 
TCTTCGGCAG ACACTTTTAC GATCTCTGGG GTGTCTACAA ATAAGGAAAA TGAGGGCCCG 
GAGTATCCAA CCAAAATTGA GAAAAATCAA TTCAATATGT CATATAAACC ATCTGAGAAT 
ATGTCAGGGC TCTCTTCATT TCCTATCTTT GAAAAAGAAA ACACGTTAAG CTCAAGTTAT 
TTGGAAGAAC AGAAGCCAAA GAGAGCGGCC CTTTCAGATA TCACGAACTC ATTCAATAAA 
ATGAATAAAC AGGAAGGTAT GAGGATAGAA AAAAAGATTC AAAGAGAACA ACTTCAAAAA 
AAAAATGACC GCCCGTCACC ACTCAAACCT ATTCAGCACC AAGAACTTCG TGTAAATTCT 
TTGCCTAATG ATCAAGGTAA ACCGTCCTTA TCTCTAGATC CCCGCCGTAA CATCTCCCAG 
CCAGTGAATT CAAAAGTGGA ATCACTATTG CAGGGACTGA AATTCAAAAA GGAGCCAGCC 
TCTCACTGGA CCCATGAGAG AGGTTCCTTG TTCATGAGTG AACATGTGGA AGACGAAAAA 
CCAGTCAAAG CCTCAGATGT TTCAATCGAA AGTTCATATG TACCTTTAAC AACAGTAGCA 
ACATCTTCGA GAGATCCCAG TGTTTTAGCA GAATCTAGTA CAATTCAAAA ACCAATGCTG 
TCTTTACCTT CTAGTTTTTT GAACACATCT ATGACGTTCA AAAATCTTAG CCAGATACTC 
GCTGATGACG GTGATGATAA GCATCTAAGT GTGCCACAGA ACCAATCTAG AAGTGTAGCC 
ATGTCACACC CTTTGCGAAA ACAATCTGCC AAAATATCTC TAACTCCACG GTCTAATTTG 
AACGCCAATT TATCCGTTAA ACGTAATCAG GGCTCACCTG GCTCCTATTT GAGTAATGAT 
CTGGATGGAA TCTCTGATAT GACATTTGCG ATGGAAATAC CAACAAACAC CTTTACCGCT 
CAGGCGATTC AACTGATGAA CAACGACACG GACAATAATA AAATCAATAC TTCGCCCAAA 
GCATCTTCTT TTACAAAAGA AAAAGTAATC AAATCGGCAG CCTATATCTC GAAGGAAAAA 
GAACCAGATA ATAGCGATAC TAATTATATT CCAGATTATA CTATACCAAA CACATATGAT 
GAAAAAGCCA TTAATATTTT TGAAGATGCG CCCTCCGATG AGGGTTCATT AAATACATCT 
TCTTCAGAAA GCGATTCACG AGCAAGTGTG CATAGGAAAG CGGTATCTAT TGACACTATG 
GCGACAACAA ACGTTCTTAC TCCCGCGACA AATGTTAGAG TAAGTCTTTA TTGGAATAAT 
AACAGTTCTG GTATACCCAG AGAGACTACT GAAGAGATAC TTTCTAAACT TAGATTGTCA 
CCAGAAAATC CTTCTAATAC ACATATGCAA AAGAGGTTTT CTTCCACGCG TGGTAGTCGT 
GATAGTAATG CATTAGGCAT TTCTCAAAGT TTACAGTCAA TGTTCAAAGA TTTGGAGGAA 
GATCAAGATG GGCATACTTC TCAAGCTGAT ATTCTTGAAT CAAGTATGAG CTATTCAAAG 
CGAAGACCAT CTGAGGAAAG CGTTAATCCA AAACAACGAG TGACAATGCT TTTCGATGAG 
GAGGAGGAAG AATCTAAAAA AGTAGGTGGG GGAAAAATTA AAGAAGAGCA TACCAAGCTA 
GACAATAAAA TATCCGAGGA ATCTTCTCAA CTTGTGTTGC CGGTGGTTGA AAAAAAGGAA 
AATGCAAATA ATACAGAAAA TAACTACTCT AAAATACCAA AACCCTCAAC GATTAAAGTT 
ACGAAGGATA CCGCTATGGA ATCAAATACA CAAACCCATA CAAAAAAGCC AATTCTAAAA 
AGTGTCCAAA ATGTTGAAGT TGAAGAAGCG CCTTCCAGTG ATAAAAAGAA TTGGTTCGTC 
AAACTATTTC AAAACTTTTC TTCTCATAAT AATGCTACTA AGGCATCCAA GAATCATGTA 
ACGAATATAT CATTTGATGA TGCGCATATG CTAACATTAA ATGAATTCAA CAAGAATAGT 
ATCGACTATC AATTAAAGAA CCTAGATCAC AAATTTGGGA GAAAGGTGGT GGAATACGAT 
TGTAAATTTG TAAAAGGCAA CTTCAAATTC AAAATCAAGA TTACTAGCAC ACCCAATGCA 
TCTACGGTAA TTACTGTAAA AAAAAGAAGC AAACATTCAA ACACAAGTTC CAATAAAGCC 
TTTGAAAAGT TTAATGATGA TGTGGAGAGA GTAATTCGAA ATGCCGGACG TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012821.2
CDS	1..4557
Translation	MTGHVSKTSHVPKGRPSSLAKKAAKRAMAKVNSNPKRASGHLERVVQSVNDATKRLSQPDSTVSVATKSSKRKSRDTVGPWKLGKTLGKGSSGRVRLAKNMETGQLAAIKIVPKKKAFVHCSNNGTVPNSYSSSMVTSNVSSPSIASREHSNHSQTNPYGIEREIVIMKLISHTNVMALFEVWENKSELYLVLEYVDGGELFDYLVSKGKLPEREAIHYFKQIVEGVSYCHSFNICHRDLKPENLLLDKKNRRIKIADFGMAALELPNKLLKTSCGSPHYASPEIVMGRPYHGGPSDVWSCGIVLFALLTGHLPFNDDNIKKLLLKVQSGKYQMPSNLSSEARDLISKILVIDPEKRITTQEILKHPLIKKYDDLPVNKVLRKMRKDNMARGKSNSDLHLLNNVSPSIVTLHSKGEIDESILRSLQILWHGVSRELITAKLLQKPMSEEKLFYSLLLQYKQRHSISLSSSSENKKSATESSVNEPRIEYASKTANNTGLRSENNDVKTLHSLEIHSEDTSTVNQNNAITGVNTEINAPVLAQKSQFSINTLSQPESDKAEAEAVTLPPAIPIFNASSSRIFRNSYTSISSRSRRSLRLSNSRLSLSASTSRETVHDNEMPLPQLPKSPSRYSLSRRAIHASPSTKSIHKSLSRKNIAATVAARRTLQNSASKRSLYSLQSISKRSLNLNDLLVFDDPLPSKKPASENVNKSEPHSLESDSDFEILCDQILFGNALDRILEEEEDNEKERDTQRQRQNDTKSSADTFTISGVSTNKENEGPEYPTKIEKNQFNMSYKPSENMSGLSSFPIFEKENTLSSSYLEEQKPKRAALSDITNSFNKMNKQEGMRIEKKIQREQLQKKNDRPSPLKPIQHQELRVNSLPNDQGKPSLSLDPRRNISQPVNSKVESLLQGLKFKKEPASHWTHERGSLFMSEHVEDEKPVKASDVSIESSYVPLTTVATSSRDPSVLAESSTIQKPMLSLPSSFLNTSMTFKNLSQILADDGDDKHLSVPQNQSRSVAMSHPLRKQSAKISLTPRSNLNANLSVKRNQGSPGSYLSNDLDGISDMTFAMEIPTNTFTAQAIQLMNNDTDNNKINTSPKASSFTKEKVIKSAAYISKEKEPDNSDTNYIPDYTIPNTYDEKAINIFEDAPSDEGSLNTSSSESDSRASVHRKAVSIDTMATTNVLTPATNVRVSLYWNNNSSGIPRETTEEILSKLRLSPENPSNTHMQKRFSSTRGSRDSNALGISQSLQSMFKDLEEDQDGHTSQADILESSMSYSKRRPSEESVNPKQRVTMLFDEEEEESKKVGGGKIKEEHTKLDNKISEESSQLVLPVVEKKENANNTENNYSKIPKPSTIKVTKDTAMESNTQTHTKKPILKSVQNVEVEEAPSSDKKNWFVKLFQNFSSHNNATKASKNHVTNISFDDAHMLTLNEFNKNSIDYQLKNLDHKFGRKVVEYDCKFVKGNFKFKIKITSTPNASTVITVKKRSKHSNTSSNKAFEKFNDDVERVIRNAGRS

Target ORF information:

RefSeq Version	NM_001179667.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase HSL1 (HSL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179667.2

ORF Insert Sequence:

ATGACTGGTC ACGTTTCAAA AACGAGCCAC GTACCCAAAG GTCGCCCGTC TTCATTGGCC 
AAGAAGGCAG CTAAAAGGGC CATGGCCAAA GTAAATTCGA ATCCAAAAAG GGCGTCGGGG 
CATTTGGAGC GTGTTGTACA ATCTGTGAAC GACGCTACCA AAAGATTATC TCAGCCCGAC 
TCTACCGTAA GTGTTGCTAC AAAATCATCA AAGAGAAAAT CCAGAGACAC GGTGGGTCCT 
TGGAAACTTG GCAAAACATT GGGGAAGGGA TCTTCTGGTA GGGTTCGCTT GGCTAAAAAT 
ATGGAAACAG GACAATTAGC TGCCATTAAA ATTGTCCCCA AGAAGAAAGC CTTTGTTCAT 
TGTTCTAATA ATGGAACTGT TCCTAATTCT TACTCTTCTT CCATGGTCAC TTCCAATGTA 
TCTTCTCCAT CAATAGCATC TAGAGAACAT AGTAACCATT CTCAAACGAA CCCATATGGC 
ATAGAGAGAG AAATAGTAAT CATGAAATTG ATATCTCACA CAAACGTTAT GGCATTGTTT 
GAAGTTTGGG AGAACAAATC AGAGCTGTAC CTAGTTTTGG AATATGTTGA TGGTGGCGAA 
TTATTCGATT ATCTGGTCTC GAAGGGAAAG CTACCTGAAA GGGAGGCTAT CCATTACTTC 
AAGCAGATAG TTGAAGGTGT TTCCTATTGT CATTCTTTTA ATATATGCCA TCGTGATCTG 
AAGCCTGAAA ATTTATTATT AGATAAAAAG AATAGGAGAA TCAAAATTGC TGATTTCGGC 
ATGGCAGCAT TAGAGCTTCC TAATAAACTT TTAAAAACTT CATGTGGCTC TCCACACTAT 
GCGTCTCCTG AAATTGTTAT GGGTAGACCA TATCATGGCG GTCCTAGCGA TGTCTGGTCC 
TGCGGTATCG TTTTATTTGC ATTACTCACC GGTCATTTAC CATTTAATGA TGACAACATC 
AAAAAGCTGT TGTTGAAAGT TCAATCGGGC AAATATCAAA TGCCCTCAAA TTTATCTTCT 
GAGGCTCGTG ATTTGATATC AAAAATATTG GTAATTGATC CAGAAAAAAG AATTACCACT 
CAAGAGATAT TGAAGCATCC TCTGATCAAA AAATATGATG ATCTCCCAGT TAATAAAGTG 
CTGAGGAAAA TGAGAAAGGA TAACATGGCG AGGGGAAAAT CTAACTCGGA TTTGCATTTG 
CTAAATAATG TCTCGCCCTC TATTGTAACT TTACATTCAA AAGGGGAAAT TGATGAATCC 
ATCTTGAGGA GTTTGCAAAT TTTGTGGCAT GGAGTTTCTC GTGAACTAAT CACTGCCAAG 
TTGCTTCAAA AGCCAATGTC GGAGGAAAAA CTATTCTACT CACTGTTATT ACAATATAAA 
CAACGTCACT CAATATCATT GTCCTCGTCA AGCGAAAACA AGAAATCAGC AACAGAAAGT 
AGCGTAAATG AACCTAGGAT TGAATACGCA TCCAAAACTG CCAATAATAC AGGCCTAAGG 
AGCGAGAACA ATGATGTGAA GACTTTACAT TCTCTAGAAA TTCATTCAGA AGATACTTCA 
ACAGTGAATC AGAACAACGC AATTACAGGG GTTAATACAG AAATTAATGC CCCTGTATTG 
GCACAAAAAT CGCAATTTAG TATCAATACG CTCAGTCAAC CTGAAAGTGA TAAGGCTGAA 
GCAGAAGCTG TAACATTACC ACCCGCTATT CCCATATTCA ATGCTTCTTC ATCAAGGATT 
TTCCGTAACT CATACACCTC TATTTCATCA CGTTCGAGAA GATCGCTACG CTTATCGAAT 
TCCAGATTAT CATTGTCCGC TTCAACTTCT AGAGAAACTG TGCATGATAA TGAGATGCCT 
CTACCTCAAC TGCCGAAGTC GCCTTCAAGA TATTCATTGT CAAGAAGGGC AATCCACGCC 
TCCCCATCTA CTAAATCAAT ACATAAATCA CTTTCGAGGA AGAATATAGC TGCAACTGTT 
GCCGCTAGAA GAACACTACA AAACTCGGCT TCAAAGAGAT CCTTATACTC ATTACAGTCA 
ATTTCAAAAC GTTCCTTGAA CCTGAATGAT TTATTAGTCT TTGACGATCC TCTCCCTAGT 
AAGAAACCAG CATCCGAAAA TGTGAATAAA TCAGAACCCC ATTCGTTGGA ATCAGACTCT 
GATTTTGAGA TCTTATGCGA TCAGATCTTA TTTGGAAACG CCTTAGATAG GATACTCGAA 
GAGGAAGAGG ACAATGAAAA AGAACGCGAC ACCCAAAGAC AGAGGCAAAA CGATACAAAA 
TCTTCGGCAG ACACTTTTAC GATCTCTGGG GTGTCTACAA ATAAGGAAAA TGAGGGCCCG 
GAGTATCCAA CCAAAATTGA GAAAAATCAA TTCAATATGT CATATAAACC ATCTGAGAAT 
ATGTCAGGGC TCTCTTCATT TCCTATCTTT GAAAAAGAAA ACACGTTAAG CTCAAGTTAT 
TTGGAAGAAC AGAAGCCAAA GAGAGCGGCC CTTTCAGATA TCACGAACTC ATTCAATAAA 
ATGAATAAAC AGGAAGGTAT GAGGATAGAA AAAAAGATTC AAAGAGAACA ACTTCAAAAA 
AAAAATGACC GCCCGTCACC ACTCAAACCT ATTCAGCACC AAGAACTTCG TGTAAATTCT 
TTGCCTAATG ATCAAGGTAA ACCGTCCTTA TCTCTAGATC CCCGCCGTAA CATCTCCCAG 
CCAGTGAATT CAAAAGTGGA ATCACTATTG CAGGGACTGA AATTCAAAAA GGAGCCAGCC 
TCTCACTGGA CCCATGAGAG AGGTTCCTTG TTCATGAGTG AACATGTGGA AGACGAAAAA 
CCAGTCAAAG CCTCAGATGT TTCAATCGAA AGTTCATATG TACCTTTAAC AACAGTAGCA 
ACATCTTCGA GAGATCCCAG TGTTTTAGCA GAATCTAGTA CAATTCAAAA ACCAATGCTG 
TCTTTACCTT CTAGTTTTTT GAACACATCT ATGACGTTCA AAAATCTTAG CCAGATACTC 
GCTGATGACG GTGATGATAA GCATCTAAGT GTGCCACAGA ACCAATCTAG AAGTGTAGCC 
ATGTCACACC CTTTGCGAAA ACAATCTGCC AAAATATCTC TAACTCCACG GTCTAATTTG 
AACGCCAATT TATCCGTTAA ACGTAATCAG GGCTCACCTG GCTCCTATTT GAGTAATGAT 
CTGGATGGAA TCTCTGATAT GACATTTGCG ATGGAAATAC CAACAAACAC CTTTACCGCT 
CAGGCGATTC AACTGATGAA CAACGACACG GACAATAATA AAATCAATAC TTCGCCCAAA 
GCATCTTCTT TTACAAAAGA AAAAGTAATC AAATCGGCAG CCTATATCTC GAAGGAAAAA 
GAACCAGATA ATAGCGATAC TAATTATATT CCAGATTATA CTATACCAAA CACATATGAT 
GAAAAAGCCA TTAATATTTT TGAAGATGCG CCCTCCGATG AGGGTTCATT AAATACATCT 
TCTTCAGAAA GCGATTCACG AGCAAGTGTG CATAGGAAAG CGGTATCTAT TGACACTATG 
GCGACAACAA ACGTTCTTAC TCCCGCGACA AATGTTAGAG TAAGTCTTTA TTGGAATAAT 
AACAGTTCTG GTATACCCAG AGAGACTACT GAAGAGATAC TTTCTAAACT TAGATTGTCA 
CCAGAAAATC CTTCTAATAC ACATATGCAA AAGAGGTTTT CTTCCACGCG TGGTAGTCGT 
GATAGTAATG CATTAGGCAT TTCTCAAAGT TTACAGTCAA TGTTCAAAGA TTTGGAGGAA 
GATCAAGATG GGCATACTTC TCAAGCTGAT ATTCTTGAAT CAAGTATGAG CTATTCAAAG 
CGAAGACCAT CTGAGGAAAG CGTTAATCCA AAACAACGAG TGACAATGCT TTTCGATGAG 
GAGGAGGAAG AATCTAAAAA AGTAGGTGGG GGAAAAATTA AAGAAGAGCA TACCAAGCTA 
GACAATAAAA TATCCGAGGA ATCTTCTCAA CTTGTGTTGC CGGTGGTTGA AAAAAAGGAA 
AATGCAAATA ATACAGAAAA TAACTACTCT AAAATACCAA AACCCTCAAC GATTAAAGTT 
ACGAAGGATA CCGCTATGGA ATCAAATACA CAAACCCATA CAAAAAAGCC AATTCTAAAA 
AGTGTCCAAA ATGTTGAAGT TGAAGAAGCG CCTTCCAGTG ATAAAAAGAA TTGGTTCGTC 
AAACTATTTC AAAACTTTTC TTCTCATAAT AATGCTACTA AGGCATCCAA GAATCATGTA 
ACGAATATAT CATTTGATGA TGCGCATATG CTAACATTAA ATGAATTCAA CAAGAATAGT 
ATCGACTATC AATTAAAGAA CCTAGATCAC AAATTTGGGA GAAAGGTGGT GGAATACGAT 
TGTAAATTTG TAAAAGGCAA CTTCAAATTC AAAATCAAGA TTACTAGCAC ACCCAATGCA 
TCTACGGTAA TTACTGTAAA AAAAAGAAGC AAACATTCAA ACACAAGTTC CAATAAAGCC 
TTTGAAAAGT TTAATGATGA TGTGGAGAGA GTAATTCGAA ATGCCGGACG TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
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HSL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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