STE6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STE6
Entrez Gene ID	853671
Full Name	ATP-binding cassette a-factor transporter STE6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179774.1	NP_012713.1	ATP-binding cassette a-factor transporter STE6

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mam1	NP_001018819.2
Neurospora crassa	NCU09443	XP_958595.1
Saccharomyces cerevisiae (baker's yeast)	STE6	NP_012713.1
Kluyveromyces lactis	KLLA0B14256g	XP_452165.1
Magnaporthe oryzae (rice blast fungus)	MGG_04899	XP_003712386.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hsp70/40s act before ubiquitination and facilitate Ste6p association with E3 ubiquitin ligase, suggesting an active role for chaperones. Furthermore, polyubiquitination was a prerequisite for retrotranslocation, which required the Cdc48 complex and ATP.
Title: Dissecting the ER-associated degradation of a misfolded polytopic membrane protein.

STE6 recycling is controlled by ubiquinination in the early endocytic pathway.
Title: Control of Ste6 recycling by ubiquitination in the early endocytic pathway in yeast.

Ubp1 has a role in sorting the ATP-binding cassette-transporter Ste6 in the endocytic pathway
Title: The deubiquitinating enzyme Ubp1 affects sorting of the ATP-binding cassette-transporter Ste6 in the endocytic pathway.

Ste6p follows the PURE pathway--a general pathway for the internalization of plasma membrane proteins that involves phosphorylation, ubiquitination, recognition and endocytosis
Title: The internalization of yeast Ste6p follows an ordered series of events involving phosphorylation, ubiquitination, recognition and endocytosis.

The following STE6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STE6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02891	NM_001179774.1 Latest version!	Saccharomyces cerevisiae S288C ATP-binding cassette a-factor transporter STE6 (STE6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STE6 ( NM_001179774.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012713.1 Saccharomyces cerevisiae S288C ATP-binding cassette a-factor transporter STE6 (STE6), partial mRNA.

Price & Availability↑

CloneID	OSi02891
Clone ID Related Accession (Same CDS sequence)	NM_001179774.1
Accession Version	NM_001179774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-binding cassette a-factor transporter STE6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACTTTT TAAGTTTTAA GACTACAAAA CACTATCACA TTTTCAGGTA CGTGAACATA 
CGGAATGACT ACAGGCTGTT AATGATAATG ATAATAGGTA CCGTGGCAAC AGGCCTAGTG 
CCGGCAATTA CTTCTATCCT GACGGGCAGA GTGTTCGATC TACTATCAGT TTTCGTGGCT 
AATGGGTCAC ATCAAGGTTT GTATTCCCAA CTAGTACAGA GGTCAATGGC AGTAATGGCA 
CTTGGTGCGG CTTCTGTGCC AGTAATGTGG CTTTCTCTAA CAAGTTGGAT GCACATCGGC 
GAGAGACAAG GCTTTAGAAT ACGGTCACAG ATATTGGAGG CATATTTGGA GGAAAAGCCA 
ATGGAATGGT ACGACAATAA TGAAAAATTG TTAGGAGATT TTACTCAAAT CAACAGATGT 
GTGGAAGAGC TAAGATCAAG CTCCGCAGAG GCATCAGCCA TAACTTTCCA GAATTTAGTT 
GCAATATGTG CGCTTCTGGG GACGTCATTC TACTATTCTT GGTCATTAAC TTTAATTATT 
CTTTGCAGCT CTCCAATAAT CACATTTTTT GCAGTGGTGT TTTCCAGAAT GATTCATGTA 
TATTCAGAGA AGGAGAATTC TGAAACGAGT AAAGCAGCCC AATTACTTAC ATGGTCGATG 
AATGCCGCTC AATTAGTGAG ATTATATTGT ACACAACGTC TAGAAAGGAA AAAATTCAAG 
GAAATCATAC TAAATTGTAA CACTTTCTTC ATCAAGAGTT GCTTTTTTGT TGCTGCAAAC 
GCTGGGATCT TGAGATTTTT GACGTTGACT ATGTTTGTAC AGGGATTCTG GTTTGGTTCC 
GCAATGATCA AAAAGGGCAA GCTGAACATT AACGATGTAA TCACTTGCTT CCATTCATGC 
ATTATGCTGG GCTCGACTTT AAATAATACA TTACACCAAA TAGTTGTTCT TCAAAAAGGC 
GGAGTGGCTA TGGAAAAAAT CATGACTCTA TTAAAAGATG GATCCAAGCG AAATCCTTTA 
AATAAAACTG TAGCCCACCA ATTTCCACTA GATTATGCCA CCAGTGATCT AACATTTGCT 
AATGTTTCGT TTTCTTATCC AAGCAGACCT TCGGAAGCAG TTTTAAAGAA CGTTAGTTTA 
AATTTCTCTG CAGGACAATT TACTTTCATA GTAGGAAAAT CAGGCTCAGG TAAATCTACA 
TTATCCAACT TATTATTAAG GTTCTACGAT GGCTATAATG GATCGATATC TATCAATGGC 
CACAATATCC AAACAATCGA CCAAAAATTG CTAATTGAAA ATATCACCGT CGTAGAACAG 
CGTTGTACCC TATTTAATGA TACTCTTAGA AAAAATATTC TTTTAGGTTC AACAGATTCG 
GTAAGAAATG CCGATTGCAG CACCAATGAA AATAGACATT TAATCAAAGA TGCCTGTCAA 
ATGGCTCTTC TGGATAGATT TATTCTAGAC CTTCCCGATG GACTGGAAAC ATTAATTGGG 
ACTGGGGGTG TCACACTAAG TGGCGGGCAA CAACAAAGAG TTGCTATAGC ACGTGCATTC 
ATCAGAGATA CTCCAATATT ATTCTTAGAC GAAGCTGTAT CGGCTCTAGA TATTGTTCAT 
CGCAACCTGT TGATGAAGGC AATTAGGCAT TGGAGGAAAG GAAAGACTAC AATCATATTG 
ACGCATGAGT TGAGCCAAAT TGAATCTGAT GACTATTTAT ATTTAATGAA GGAAGGTGAA 
GTTGTTGAAA GCGGCACCCA GTCTGAACTT CTAGCCGATC CGACCACTAC ATTTAGCACA 
TGGTATCACC TACAGAATGA CTACTCTGAT GCGAAAACTA TTGTAGATAC AGAGACTGAA 
GAAAAATCTA TACACACTGT GGAAAGTTTT AACTCTCAAT TGGAAACACC AAAACTTGGA 
TCATGCTTAA GTAATCTGGG ATATGATGAG ACAGATCAGT TGTCCTTTTA CGAAGCAATC 
TATCAAAAAA GATCGAACGT TAGAACAAGA AGGGTTAAAG TTGAAGAGGA AAATATTGGG 
TATGCACTAA AACAACAAAA GAACACCGAA AGTTCAACAG GGCCACAACT TCTGAGCATT 
ATTCAGATTA TCAAAAGAAT GATTAAAAGC ATAAGATACA AAAAAATTCT AATCTTGGGA 
CTGCTATGTT CTCTTATCGC AGGCGCCACA AATCCCGTCT TTTCATACAC ATTCAGTTTC 
TTACTAGAAG GAATTGTCCC ATCCACGGAT GGAAAAACTG GCTCTTCACA TTATTTGGCG 
AAATGGTCGC TTCTTGTTCT TGGTGTGGCT GCGGCAGATG GTATTTTCAA TTTTGCTAAA 
GGATTCCTAT TAGATTGCTG CAGTGAATAC TGGGTTATGG ATCTTAGAAA TGAAGTTATG 
GAAAAACTGA CGAGAAAGAA TATGGACTGG TTTTCTGGTG AAAACAACAA GGCTTCTGAA 
ATTTCTGCTC TAGTCTTGAA TGATTTGCGA GATTTGAGGT CTTTGGTCTC TGAATTTTTG 
AGTGCAATGA CTAGTTTCGT TACCGTATCA ACGATTGGAC TAATTTGGGC GTTAGTATCG 
GGCTGGAAGT TAAGTTTGGT TTGTATTTCG ATGTTTCCAC TCATAATTAT ATTTTCAGCA 
ATATATGGAG GCATTTTACA AAAATGCGAA ACAGATTATA AGACATCTGT TGCTCAGTTA 
GAAAACTGCC TGTACCAGAT TGTCACTAAC ATTAAAACCA TTAAGTGCTT ACAAGCTGAA 
TTTCATTTTC AATTGACCTA CCATGACTTG AAGATAAAAA TGCAACAAAT TGCCTCCAAA 
CGCGCCATTG CCACAGGATT TGGTATATCT ATGACAAACA TGATTGTCAT GTGTATCCAA 
GCTATTATTT ACTACTATGG CCTAAAGCTG GTTATGATTC ACGAGTACAC CTCAAAGGAA 
ATGTTTACGA CTTTCACTTT GTTATTATTC ACTATTATGT CATGCACTAG CCTAGTAAGT 
CAGATACCCG ATATAAGTAG AGGCCAACGT GCTGCCAGTT GGATCTATAG GATTCTTGAT 
GAAAAGCATA ATACCCTAGA GGTTGAAAAC AATAATGCTA GAACAGTGGG AATAGCTGGT 
CACACCTACC ATGGCAAAGA AAAAAAACCA ATCGTTTCAA TTCAAAATTT GACATTTGCC 
TATCCATCTG CACCTACCGC CTTTGTTTAC AAAAACATGA ATTTTGACAT GTTTTGCGGA 
CAGACGTTAG GTATCATTGG TGAATCAGGC ACAGGAAAGT CTACACTTGT GCTTTTATTA 
ACAAAACTTT ATAATTGTGA AGTAGGCAAA ATTAAAATAG ACGGTACGGA CGTAAATGAC 
TGGAATTTGA CAAGTTTAAG AAAAGAAATT TCAGTGGTTG AGCAAAAACC TTTATTATTC 
AATGGAACCA TCAGAGATAA CCTAACTTAT GGTTTACAAG ATGAAATACT TGAAATTGAA 
ATGTATGATG CATTAAAATA CGTAGGAATC CATGACTTTG TAATTTCATC ACCTCAGGGC 
TTGGATACAC GTATTGATAC AACTTTACTG TCAGGTGGAC AAGCGCAAAG GCTTTGCATA 
GCCAGAGCAC TTCTGAGAAA ATCAAAAATT CTGATTTTAG ATGAGTGTAC TTCAGCCTTG 
GATTCTGTCA GCTCCTCTAT CATCAATGAG ATCGTCAAAA AAGGTCCACC TGCTCTACTA 
ACAATGGTTA TAACGCATAG TGAACAAATG ATGAGGTCTT GTAACTCGAT TGCAGTTCTT 
AAAGATGGTA AAGTGGTTGA GCGAGGTAAC TTCGACACTT TATATAATAA TCGCGGGGAA 
TTATTCCAAA TTGTTTCCAA CCAAAGCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012713.1
CDS	1..3873
Translation	MNFLSFKTTKHYHIFRYVNIRNDYRLLMIMIIGTVATGLVPAITSILTGRVFDLLSVFVANGSHQGLYSQLVQRSMAVMALGAASVPVMWLSLTSWMHIGERQGFRIRSQILEAYLEEKPMEWYDNNEKLLGDFTQINRCVEELRSSSAEASAITFQNLVAICALLGTSFYYSWSLTLIILCSSPIITFFAVVFSRMIHVYSEKENSETSKAAQLLTWSMNAAQLVRLYCTQRLERKKFKEIILNCNTFFIKSCFFVAANAGILRFLTLTMFVQGFWFGSAMIKKGKLNINDVITCFHSCIMLGSTLNNTLHQIVVLQKGGVAMEKIMTLLKDGSKRNPLNKTVAHQFPLDYATSDLTFANVSFSYPSRPSEAVLKNVSLNFSAGQFTFIVGKSGSGKSTLSNLLLRFYDGYNGSISINGHNIQTIDQKLLIENITVVEQRCTLFNDTLRKNILLGSTDSVRNADCSTNENRHLIKDACQMALLDRFILDLPDGLETLIGTGGVTLSGGQQQRVAIARAFIRDTPILFLDEAVSALDIVHRNLLMKAIRHWRKGKTTIILTHELSQIESDDYLYLMKEGEVVESGTQSELLADPTTTFSTWYHLQNDYSDAKTIVDTETEEKSIHTVESFNSQLETPKLGSCLSNLGYDETDQLSFYEAIYQKRSNVRTRRVKVEEENIGYALKQQKNTESSTGPQLLSIIQIIKRMIKSIRYKKILILGLLCSLIAGATNPVFSYTFSFLLEGIVPSTDGKTGSSHYLAKWSLLVLGVAAADGIFNFAKGFLLDCCSEYWVMDLRNEVMEKLTRKNMDWFSGENNKASEISALVLNDLRDLRSLVSEFLSAMTSFVTVSTIGLIWALVSGWKLSLVCISMFPLIIIFSAIYGGILQKCETDYKTSVAQLENCLYQIVTNIKTIKCLQAEFHFQLTYHDLKIKMQQIASKRAIATGFGISMTNMIVMCIQAIIYYYGLKLVMIHEYTSKEMFTTFTLLLFTIMSCTSLVSQIPDISRGQRAASWIYRILDEKHNTLEVENNNARTVGIAGHTYHGKEKKPIVSIQNLTFAYPSAPTAFVYKNMNFDMFCGQTLGIIGESGTGKSTLVLLLTKLYNCEVGKIKIDGTDVNDWNLTSLRKEISVVEQKPLLFNGTIRDNLTYGLQDEILEIEMYDALKYVGIHDFVISSPQGLDTRIDTTLLSGGQAQRLCIARALLRKSKILILDECTSALDSVSSSIINEIVKKGPPALLTMVITHSEQMMRSCNSIAVLKDGKVVERGNFDTLYNNRGELFQIVSNQSS

Target ORF information:

RefSeq Version	NM_001179774.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-binding cassette a-factor transporter STE6 (STE6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179774.1

ORF Insert Sequence:

ATGAACTTTT TAAGTTTTAA GACTACAAAA CACTATCACA TTTTCAGGTA CGTGAACATA 
CGGAATGACT ACAGGCTGTT AATGATAATG ATAATAGGTA CCGTGGCAAC AGGCCTAGTG 
CCGGCAATTA CTTCTATCCT GACGGGCAGA GTGTTCGATC TACTATCAGT TTTCGTGGCT 
AATGGGTCAC ATCAAGGTTT GTATTCCCAA CTAGTACAGA GGTCAATGGC AGTAATGGCA 
CTTGGTGCGG CTTCTGTGCC AGTAATGTGG CTTTCTCTAA CAAGTTGGAT GCACATCGGC 
GAGAGACAAG GCTTTAGAAT ACGGTCACAG ATATTGGAGG CATATTTGGA GGAAAAGCCA 
ATGGAATGGT ACGACAATAA TGAAAAATTG TTAGGAGATT TTACTCAAAT CAACAGATGT 
GTGGAAGAGC TAAGATCAAG CTCCGCAGAG GCATCAGCCA TAACTTTCCA GAATTTAGTT 
GCAATATGTG CGCTTCTGGG GACGTCATTC TACTATTCTT GGTCATTAAC TTTAATTATT 
CTTTGCAGCT CTCCAATAAT CACATTTTTT GCAGTGGTGT TTTCCAGAAT GATTCATGTA 
TATTCAGAGA AGGAGAATTC TGAAACGAGT AAAGCAGCCC AATTACTTAC ATGGTCGATG 
AATGCCGCTC AATTAGTGAG ATTATATTGT ACACAACGTC TAGAAAGGAA AAAATTCAAG 
GAAATCATAC TAAATTGTAA CACTTTCTTC ATCAAGAGTT GCTTTTTTGT TGCTGCAAAC 
GCTGGGATCT TGAGATTTTT GACGTTGACT ATGTTTGTAC AGGGATTCTG GTTTGGTTCC 
GCAATGATCA AAAAGGGCAA GCTGAACATT AACGATGTAA TCACTTGCTT CCATTCATGC 
ATTATGCTGG GCTCGACTTT AAATAATACA TTACACCAAA TAGTTGTTCT TCAAAAAGGC 
GGAGTGGCTA TGGAAAAAAT CATGACTCTA TTAAAAGATG GATCCAAGCG AAATCCTTTA 
AATAAAACTG TAGCCCACCA ATTTCCACTA GATTATGCCA CCAGTGATCT AACATTTGCT 
AATGTTTCGT TTTCTTATCC AAGCAGACCT TCGGAAGCAG TTTTAAAGAA CGTTAGTTTA 
AATTTCTCTG CAGGACAATT TACTTTCATA GTAGGAAAAT CAGGCTCAGG TAAATCTACA 
TTATCCAACT TATTATTAAG GTTCTACGAT GGCTATAATG GATCGATATC TATCAATGGC 
CACAATATCC AAACAATCGA CCAAAAATTG CTAATTGAAA ATATCACCGT CGTAGAACAG 
CGTTGTACCC TATTTAATGA TACTCTTAGA AAAAATATTC TTTTAGGTTC AACAGATTCG 
GTAAGAAATG CCGATTGCAG CACCAATGAA AATAGACATT TAATCAAAGA TGCCTGTCAA 
ATGGCTCTTC TGGATAGATT TATTCTAGAC CTTCCCGATG GACTGGAAAC ATTAATTGGG 
ACTGGGGGTG TCACACTAAG TGGCGGGCAA CAACAAAGAG TTGCTATAGC ACGTGCATTC 
ATCAGAGATA CTCCAATATT ATTCTTAGAC GAAGCTGTAT CGGCTCTAGA TATTGTTCAT 
CGCAACCTGT TGATGAAGGC AATTAGGCAT TGGAGGAAAG GAAAGACTAC AATCATATTG 
ACGCATGAGT TGAGCCAAAT TGAATCTGAT GACTATTTAT ATTTAATGAA GGAAGGTGAA 
GTTGTTGAAA GCGGCACCCA GTCTGAACTT CTAGCCGATC CGACCACTAC ATTTAGCACA 
TGGTATCACC TACAGAATGA CTACTCTGAT GCGAAAACTA TTGTAGATAC AGAGACTGAA 
GAAAAATCTA TACACACTGT GGAAAGTTTT AACTCTCAAT TGGAAACACC AAAACTTGGA 
TCATGCTTAA GTAATCTGGG ATATGATGAG ACAGATCAGT TGTCCTTTTA CGAAGCAATC 
TATCAAAAAA GATCGAACGT TAGAACAAGA AGGGTTAAAG TTGAAGAGGA AAATATTGGG 
TATGCACTAA AACAACAAAA GAACACCGAA AGTTCAACAG GGCCACAACT TCTGAGCATT 
ATTCAGATTA TCAAAAGAAT GATTAAAAGC ATAAGATACA AAAAAATTCT AATCTTGGGA 
CTGCTATGTT CTCTTATCGC AGGCGCCACA AATCCCGTCT TTTCATACAC ATTCAGTTTC 
TTACTAGAAG GAATTGTCCC ATCCACGGAT GGAAAAACTG GCTCTTCACA TTATTTGGCG 
AAATGGTCGC TTCTTGTTCT TGGTGTGGCT GCGGCAGATG GTATTTTCAA TTTTGCTAAA 
GGATTCCTAT TAGATTGCTG CAGTGAATAC TGGGTTATGG ATCTTAGAAA TGAAGTTATG 
GAAAAACTGA CGAGAAAGAA TATGGACTGG TTTTCTGGTG AAAACAACAA GGCTTCTGAA 
ATTTCTGCTC TAGTCTTGAA TGATTTGCGA GATTTGAGGT CTTTGGTCTC TGAATTTTTG 
AGTGCAATGA CTAGTTTCGT TACCGTATCA ACGATTGGAC TAATTTGGGC GTTAGTATCG 
GGCTGGAAGT TAAGTTTGGT TTGTATTTCG ATGTTTCCAC TCATAATTAT ATTTTCAGCA 
ATATATGGAG GCATTTTACA AAAATGCGAA ACAGATTATA AGACATCTGT TGCTCAGTTA 
GAAAACTGCC TGTACCAGAT TGTCACTAAC ATTAAAACCA TTAAGTGCTT ACAAGCTGAA 
TTTCATTTTC AATTGACCTA CCATGACTTG AAGATAAAAA TGCAACAAAT TGCCTCCAAA 
CGCGCCATTG CCACAGGATT TGGTATATCT ATGACAAACA TGATTGTCAT GTGTATCCAA 
GCTATTATTT ACTACTATGG CCTAAAGCTG GTTATGATTC ACGAGTACAC CTCAAAGGAA 
ATGTTTACGA CTTTCACTTT GTTATTATTC ACTATTATGT CATGCACTAG CCTAGTAAGT 
CAGATACCCG ATATAAGTAG AGGCCAACGT GCTGCCAGTT GGATCTATAG GATTCTTGAT 
GAAAAGCATA ATACCCTAGA GGTTGAAAAC AATAATGCTA GAACAGTGGG AATAGCTGGT 
CACACCTACC ATGGCAAAGA AAAAAAACCA ATCGTTTCAA TTCAAAATTT GACATTTGCC 
TATCCATCTG CACCTACCGC CTTTGTTTAC AAAAACATGA ATTTTGACAT GTTTTGCGGA 
CAGACGTTAG GTATCATTGG TGAATCAGGC ACAGGAAAGT CTACACTTGT GCTTTTATTA 
ACAAAACTTT ATAATTGTGA AGTAGGCAAA ATTAAAATAG ACGGTACGGA CGTAAATGAC 
TGGAATTTGA CAAGTTTAAG AAAAGAAATT TCAGTGGTTG AGCAAAAACC TTTATTATTC 
AATGGAACCA TCAGAGATAA CCTAACTTAT GGTTTACAAG ATGAAATACT TGAAATTGAA 
ATGTATGATG CATTAAAATA CGTAGGAATC CATGACTTTG TAATTTCATC ACCTCAGGGC 
TTGGATACAC GTATTGATAC AACTTTACTG TCAGGTGGAC AAGCGCAAAG GCTTTGCATA 
GCCAGAGCAC TTCTGAGAAA ATCAAAAATT CTGATTTTAG ATGAGTGTAC TTCAGCCTTG 
GATTCTGTCA GCTCCTCTAT CATCAATGAG ATCGTCAAAA AAGGTCCACC TGCTCTACTA 
ACAATGGTTA TAACGCATAG TGAACAAATG ATGAGGTCTT GTAACTCGAT TGCAGTTCTT 
AAAGATGGTA AAGTGGTTGA GCGAGGTAAC TTCGACACTT TATATAATAA TCGCGGGGAA 
TTATTCCAAA TTGTTTCCAA CCAAAGCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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STE6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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