FAS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FAS1
Entrez Gene ID	853653
Full Name	tetrafunctional fatty acid synthase subunit FAS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179748.1	NP_012739.1	tetrafunctional fatty acid synthase subunit FAS1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B02717g	XP_451653.1
Magnaporthe oryzae (rice blast fungus)	MGG_04118	XP_003719712.1
Saccharomyces cerevisiae (baker's yeast)	FAS1	NP_012739.1
Eremothecium gossypii	AGOS_AER085C	NP_984945.2
Neurospora crassa	NCU07307	XP_962465.1
Schizosaccharomyces pombe (fission yeast)	fas1	NP_594370.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of FAS1, FAS2 and Acc1 leads to accumulation of sterols.
Title: Overexpression of genes of the fatty acid biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae.

Results structurally and functionally characterized the FAS type I PPT as part of the multienzyme protein and as an isolated domain.
Title: Multimeric options for the auto-activation of the Saccharomyces cerevisiae FAS type I megasynthase.

The crystal structure of the 2.6-MDa Saccharomyces cerevisiae fatty acid synthase (FAS) multienzyme in complex with the antibiotic cerulenin, is presented.
Title: Inhibition of the fungal fatty acid synthase type I multienzyme complex.

Sfh2p inhibits fatty acid synthase activity in response to haem depletion
Title: SFH2 regulates fatty acid synthase activity in the yeast Saccharomyces cerevisiae and is critical to prevent saturated fatty acid accumulation in response to haem and oleic acid depletion.

Fatty acid synthase (Fas) plays a key role during the cellular response of Saccharomyces cerevisiae to H2O2.
Title: Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells to H2O2.

The following FAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02875	NM_001179748.1 Latest version!	Saccharomyces cerevisiae S288C tetrafunctional fatty acid synthase subunit FAS1 (FAS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAS1 ( NM_001179748.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012739.1 Saccharomyces cerevisiae S288C tetrafunctional fatty acid synthase subunit FAS1 (FAS1), partial mRNA.

Price & Availability↑

CloneID	OSi02875
Clone ID Related Accession (Same CDS sequence)	NM_001179748.1
Accession Version	NM_001179748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	tetrafunctional fatty acid synthase subunit FAS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGACGCTT ACTCCACAAG ACCATTAACC CTATCTCACG GTTCTTTAGA GCACGTGCTT 
CTGGTACCAA CCGCTTCATT TTTCATTGCT TCGCAATTAC AAGAACAATT TAATAAAATT 
TTGCCCGAAC CCACTGAAGG GTTTGCTGCA GATGACGAGC CTACCACACC TGCTGAACTA 
GTGGGGAAAT TCCTTGGCTA CGTATCTTCT CTAGTCGAAC CTTCCAAGGT CGGTCAATTC 
GATCAGGTCT TGAACCTTTG CTTAACAGAA TTTGAAAACT GTTATTTAGA AGGCAATGAC 
ATTCACGCCT TGGCTGCTAA ACTATTACAG GAAAACGACA CAACTTTAGT GAAGACTAAA 
GAACTAATTA AAAATTATAT TACCGCCAGA ATAATGGCTA AGAGACCATT TGACAAAAAA 
TCCAACTCTG CTCTTTTTAG GGCCGTCGGC GAGGGTAACG CACAATTGGT AGCCATTTTC 
GGTGGTCAAG GTAACACCGA CGACTACTTT GAAGAATTGC GTGATCTATA TCAAACTTAT 
CATGTCTTAG TGGGAGATTT AATCAAGTTC TCCGCTGAAA CTTTAAGTGA ACTGATTAGA 
ACTACTTTAG ATGCTGAAAA AGTCTTTACT CAAGGTTTAA ACATATTGGA ATGGTTGGAG 
AACCCTTCAA ATACCCCAGA CAAGGACTAT TTACTTTCCA TTCCAATTTC ATGCCCCTTA 
ATTGGTGTCA TTCAATTGGC TCACTACGTA GTTACTGCCA AGCTTTTGGG TTTCACTCCA 
GGTGAGTTAA GATCTTACTT AAAAGGTGCT ACAGGTCACT CTCAAGGTTT GGTTACTGCT 
GTCGCCATAG CTGAGACGGA TTCCTGGGAA TCCTTCTTCG TCTCCGTAAG AAAAGCAATT 
ACTGTATTAT TCTTCATCGG TGTTCGTTGT TACGAAGCAT ACCCAAACAC TTCCCTACCA 
CCATCCATCT TGGAAGATTC CTTGGAAAAC AATGAAGGTG TTCCATCTCC AATGTTGTCC 
ATTTCCAATC TAACTCAAGA ACAAGTTCAA GACTATGTAA ATAAGACTAA CTCTCATTTG 
CCAGCTGGTA AACAAGTTGA AATTTCTCTA GTCAATGGTG CGAAGAATCT AGTCGTATCG 
GGCCCACCAC AATCATTATA TGGTTTAAAC TTGACTTTAA GAAAGGCCAA GGCCCCATCT 
GGACTGGATC AATCAAGAAT CCCATTCAGC GAAAGAAAAT TGAAGTTCTC CAATAGGTTC 
TTACCTGTTG CATCACCATT CCATTCCCAT CTATTGGTTC CAGCTTCAGA TTTGATTAAC 
AAAGACTTAG TCAAAAACAA TGTCAGCTTT AACGCTAAAG ATATTCAAAT CCCCGTTTAC 
GACACTTTTG ATGGTTCAGA TCTAAGAGTC CTTTCAGGTT CCATTTCCGA GAGAATCGTC 
GACTGCATCA TTAGATTACC TGTCAAATGG GAAACTACTA CACAATTCAA AGCCACCCAC 
ATATTAGACT TTGGTCCAGG TGGAGCTTCC GGTTTAGGTG TTTTAACCCA TCGTAATAAA 
GATGGTACTG GTGTTCGTGT TATCGTTGCC GGTACTCTCG ACATTAACCC AGATGATGAT 
TACGGATTCA AGCAAGAAAT CTTTGATGTT ACTAGTAATG GTTTGAAGAA AAATCCAAAC 
TGGTTGGAAG AATACCATCC AAAATTAATT AAGAACAAAT CAGGCAAAAT TTTTGTCGAA 
ACAAAATTTT CTAAATTAAT CGGTAGACCA CCTTTATTGG TTCCTGGTAT GACACCATGT 
ACTGTTTCTC CAGATTTCGT AGCTGCTACC ACAAATGCTG GTTATACCAT TGAGTTGGCC 
GGTGGTGGTT ACTTTTCCGC AGCAGGTATG ACCGCCGCTA TTGATTCTGT GGTTTCTCAG 
ATAGAAAAGG GTAGTACCTT CGGTATCAAC TTGATCTACG TCAATCCATT TATGTTACAA 
TGGGGTATTC CATTAATCAA GGAACTAAGA AGCAAAGGTT ATCCAATTCA ATTCTTGACC 
ATTGGTGCTG GTGTCCCATC ATTGGAAGTT GCTAGTGAAT ACATAGAGAC ATTAGGTTTG 
AAGTACTTGG GTTTGAAACC AGGTTCCATT GATGCTATTT CGCAAGTTAT AAACATTGCT 
AAAGCACATC CAAACTTCCC AATAGCTTTA CAATGGACCG GTGGTAGAGG TGGTGGTCAT 
CATTCTTTCG AAGATGCCCA CACTCCAATG TTACAAATGT ACTCCAAGAT TAGAAGACAT 
CCAAACATTA TGTTGATATT CGGTTCTGGT TTCGGTTCTG CTGATGACAC TTACCCATAC 
TTAACCGGTG AATGGTCCAC AAAATTCGAT TATCCACCAA TGCCATTCGA TGGTTTCCTA 
TTTGGTTCGA GGGTCATGAT TGCTAAGGAA GTTAAAACTT CTCCTGATGC TAAGAAGTGT 
ATTGCTGCTT GTACTGGTGT TCCTGATGAT AAATGGGAAC AAACCTACAA GAAGCCAACT 
GGTGGTATTG TCACTGTTCG CTCTGAAATG GGTGAACCAA TTCACAAAAT TGCCACTCGT 
GGTGTTATGC TATGGAAGGA ATTCGACGAA ACCATCTTCA ACTTACCAAA GAATAAGTTG 
GTACCAACTT TGGAAGCAAA GAGAGATTAC ATTATCTCAA GATTGAACGC CGATTTCCAA 
AAACCATGGT TTGCTACCGT CAACGGTCAA GCCCGTGACC TAGCCACAAT GACATACGAA 
GAAGTTGCAA AGAGATTGGT GGAATTAATG TTCATCAGAT CTACCAACTC TTGGTTTGAT 
GTCACATGGA GAACCTTTAC TGGTGATTTC CTACGTCGTG TCGAAGAACG TTTCACTAAA 
AGTAAGACAT TGTCTTTAAT CCAATCCTAT TCTCTACTAG ACAAGCCTGA TGAAGCTATT 
GAAAAAGTAT TTAATGCTTA TCCTGCCGCT AGGGAACAGT TCTTGAATGC GCAAGATATT 
GATCACTTTT TGAGCATGTG TCAAAATCCA ATGCAAAAAC CAGTGCCTTT TGTTCCAGTT 
TTGGATCGTA GATTCGAGAT TTTTTTCAAA AAAGATTCGT TATGGCAATC TGAGCACTTG 
GAAGCCGTCG TCGACCAAGA CGTTCAAAGA ACATGTATCC TACATGGACC TGTTGCAGCA 
CAATTCACTA AAGTCATCGA TGAACCAATT AAGAGCATTA TGGATGGTAT TCACGATGGT 
CACATCAAAA AGTTACTACA TCAATATTAC GGTGACGATG AGTCAAAGAT TCCAGCAGTT 
GAGTACTTTG GTGGTGAAAG CCCTGTAGAC GTACAAAGTC AAGTTGATTC TTCCTCTGTA 
TCTGAAGACT CAGCTGTTTT TAAGGCAACA TCCTCTACTG ATGAAGAAAG CTGGTTTAAG 
GCTTTGGCGG GATCCGAAAT TAACTGGAGA CATGCAAGTT TCTTATGTTC CTTTATCACT 
CAAGATAAAA TGTTTGTTTC TAACCCAATT AGAAAAGTTT TCAAGCCAAG CCAAGGAATG 
GTTGTTGAGA TTTCCAACGG CAATACTTCT TCAAAGACTG TTGTCACTCT TTCAGAACCT 
GTTCAAGGTG AATTGAAACC AACTGTTATT TTGAAGTTGT TGAAGGAGAA CATAATCCAA 
ATGGAAATGA TTGAGAACAG AACTATGGAT GGTAAGCCCG TCAGCTTGCC ATTGTTGTAC 
AACTTCAACC CAGATAATGG TTTTGCTCCA ATCTCTGAAG TTATGGAGGA CAGAAACCAA 
AGAATTAAGG AAATGTACTG GAAATTATGG ATTGATGAGC CTTTCAATTT GGACTTTGAC 
CCAAGAGATG TCATTAAGGG CAAAGATTTC GAGATCACCG CTAAAGAAGT TTATGACTTT 
ACACACGCTG TTGGAAACAA TTGTGAAGAC TTCGTTTCTA GACCTGATAG AACGATGTTG 
GCCCCAATGG ACTTTGCTAT TGTTGTCGGA TGGAGAGCCA TCATCAAGGC CATTTTCCCT 
AATACGGTCG ATGGTGACTT ATTGAAGTTG GTTCATTTGT CTAACGGCTA CAAGATGATT 
CCTGGCGCTA AGCCACTGCA AGTTGGTGAT GTTGTTTCAA CTACTGCTGT TATTGAATCT 
GTCGTCAACC AACCTACAGG AAAGATTGTC GATGTGGTAG GTACATTATC GAGAAATGGC 
AAGCCTGTCA TGGAAGTCAC CTCCTCATTC TTCTACAGAG GCAACTATAC TGACTTTGAA 
AACACTTTCC AAAAGACTGT TGAACCTGTT TATCAAATGC ACATCAAAAC TTCTAAAGAT 
ATAGCTGTCT TGCGCTCTAA GGAGTGGTTC CAATTGGACG ATGAAGACTT CGATCTGTTA 
AACAAAACTT TGACTTTCGA AACTGAAACT GAAGTTACTT TCAAGAATGC TAACATCTTC 
TCTTCAGTGA AATGTTTTGG CCCAATTAAA GTTGAATTGC CAACCAAAGA AACCGTGGAG 
ATCGGTATTG TCGATTACGA AGCCGGTGCC TCTCACGGTA ACCCTGTTGT TGATTTCTTG 
AAGAGAAACG GTTCCACATT GGAACAAAAG GTCAATCTAG AAAATCCTAT TCCAATTGCA 
GTACTTGATT CGTACACTCC AAGTACCAAC GAACCATACG CTAGAGTTTC TGGTGATTTG 
AATCCAATTC ACGTTTCACG TCATTTTGCC TCTTACGCAA ACTTGCCAGG TACTATCACG 
CACGGTATGT TTTCTTCTGC TTCCGTCCGT GCTTTGATTG AAAACTGGGC TGCTGACAGT 
GTTTCATCCA GGGTACGTGG CTACACTTGT CAATTTGTTG ACATGGTTTT GCCTAACACT 
GCTTTGAAAA CATCGATTCA ACATGTTGGT ATGATCAATG GTAGAAAATT GATAAAGTTT 
GAAACTAGAA ATGAAGATGA CGTTGTAGTT TTGACTGGTG AAGCCGAAAT TGAACAACCT 
GTTACTACCT TCGTTTTCAC TGGTCAAGGT TCACAAGAAC AAGGTATGGG TATGGACTTA 
TACAAAACTT CTAAAGCTGC TCAAGATGTT TGGAATAGAG CTGACAACCA TTTCAAGGAC 
ACTTATGGTT TCTCTATCTT AGACATTGTC ATTAACAACC CAGTTAACTT AACAATTCAC 
TTCGGTGGTG AAAAGGGTAA GAGGATCAGA GAAAACTATT CTGCTATGAT CTTTGAGACT 
ATCGTGGATG GAAAATTGAA GACTGAAAAA ATTTTCAAGG AAATTAATGA GCACAGTACT 
TCTTACACAT TTAGATCTGA AAAAGGTTTA TTGTCTGCTA CTCAATTTAC ACAACCAGCT 
TTAACTTTGA TGGAAAAAGC TGCTTTCGAA GACTTGAAAT CTAAAGGTTT GATCCCAGCC 
GATGCTACTT TTGCTGGTCA CTCTTTAGGT GAGTATGCTG CTTTGGCCTC TTTGGCTGAT 
GTTATGTCTA TCGAATCTTT AGTTGAAGTT GTGTTCTACA GAGGTATGAC TATGCAAGTT 
GCTGTTCCAA GAGATGAGTT GGGCAGATCC AACTATGGTA TGATTGCCAT TAACCCAGGT 
AGAGTCGCTG CATCATTCTC TCAAGAAGCT TTGCAATATG TTGTTGAGAG AGTTGGTAAG 
AGAACCGGCT GGTTGGTTGA AATCGTCAAC TACAACGTTG AAAACCAACA ATATGTTGCA 
GCTGGTGATC TAAGAGCTTT AGACACCGTT ACCAATGTTC TAAACTTCAT CAAATTACAA 
AAAATTGATA TTATTGAACT ACAAAAGTCC TTATCTTTGG AAGAAGTTGA AGGTCATTTG 
TTTGAGATCA TTGACGAAGC TTCCAAGAAA TCTGCTGTCA AGCCTCGCCC ACTTAAATTG 
GAGAGAGGTT TTGCTTGTAT CCCATTAGTT GGTATTTCTG TTCCTTTCCA TTCCACCTAC 
TTGATGAATG GTGTTAAACC ATTCAAGAGT TTCTTGAAGA AGAATATCAT AAAAGAAAAT 
GTGAAGGTTG CTAGATTGGC CGGAAAGTAC ATTCCAAACT TGACTGCAAA ACCATTCCAG 
GTTACTAAGG AATATTTCCA GGACGTTTAT GATTTGACTG GCTCCGAACC TATCAAGGAA 
ATCATCGACA ACTGGGAAAA GTATGAACAA TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012739.1
CDS	1..6156
Translation	MDAYSTRPLTLSHGSLEHVLLVPTASFFIASQLQEQFNKILPEPTEGFAADDEPTTPAELVGKFLGYVSSLVEPSKVGQFDQVLNLCLTEFENCYLEGNDIHALAAKLLQENDTTLVKTKELIKNYITARIMAKRPFDKKSNSALFRAVGEGNAQLVAIFGGQGNTDDYFEELRDLYQTYHVLVGDLIKFSAETLSELIRTTLDAEKVFTQGLNILEWLENPSNTPDKDYLLSIPISCPLIGVIQLAHYVVTAKLLGFTPGELRSYLKGATGHSQGLVTAVAIAETDSWESFFVSVRKAITVLFFIGVRCYEAYPNTSLPPSILEDSLENNEGVPSPMLSISNLTQEQVQDYVNKTNSHLPAGKQVEISLVNGAKNLVVSGPPQSLYGLNLTLRKAKAPSGLDQSRIPFSERKLKFSNRFLPVASPFHSHLLVPASDLINKDLVKNNVSFNAKDIQIPVYDTFDGSDLRVLSGSISERIVDCIIRLPVKWETTTQFKATHILDFGPGGASGLGVLTHRNKDGTGVRVIVAGTLDINPDDDYGFKQEIFDVTSNGLKKNPNWLEEYHPKLIKNKSGKIFVETKFSKLIGRPPLLVPGMTPCTVSPDFVAATTNAGYTIELAGGGYFSAAGMTAAIDSVVSQIEKGSTFGINLIYVNPFMLQWGIPLIKELRSKGYPIQFLTIGAGVPSLEVASEYIETLGLKYLGLKPGSIDAISQVINIAKAHPNFPIALQWTGGRGGGHHSFEDAHTPMLQMYSKIRRHPNIMLIFGSGFGSADDTYPYLTGEWSTKFDYPPMPFDGFLFGSRVMIAKEVKTSPDAKKCIAACTGVPDDKWEQTYKKPTGGIVTVRSEMGEPIHKIATRGVMLWKEFDETIFNLPKNKLVPTLEAKRDYIISRLNADFQKPWFATVNGQARDLATMTYEEVAKRLVELMFIRSTNSWFDVTWRTFTGDFLRRVEERFTKSKTLSLIQSYSLLDKPDEAIEKVFNAYPAAREQFLNAQDIDHFLSMCQNPMQKPVPFVPVLDRRFEIFFKKDSLWQSEHLEAVVDQDVQRTCILHGPVAAQFTKVIDEPIKSIMDGIHDGHIKKLLHQYYGDDESKIPAVEYFGGESPVDVQSQVDSSSVSEDSAVFKATSSTDEESWFKALAGSEINWRHASFLCSFITQDKMFVSNPIRKVFKPSQGMVVEISNGNTSSKTVVTLSEPVQGELKPTVILKLLKENIIQMEMIENRTMDGKPVSLPLLYNFNPDNGFAPISEVMEDRNQRIKEMYWKLWIDEPFNLDFDPRDVIKGKDFEITAKEVYDFTHAVGNNCEDFVSRPDRTMLAPMDFAIVVGWRAIIKAIFPNTVDGDLLKLVHLSNGYKMIPGAKPLQVGDVVSTTAVIESVVNQPTGKIVDVVGTLSRNGKPVMEVTSSFFYRGNYTDFENTFQKTVEPVYQMHIKTSKDIAVLRSKEWFQLDDEDFDLLNKTLTFETETEVTFKNANIFSSVKCFGPIKVELPTKETVEIGIVDYEAGASHGNPVVDFLKRNGSTLEQKVNLENPIPIAVLDSYTPSTNEPYARVSGDLNPIHVSRHFASYANLPGTITHGMFSSASVRALIENWAADSVSSRVRGYTCQFVDMVLPNTALKTSIQHVGMINGRKLIKFETRNEDDVVVLTGEAEIEQPVTTFVFTGQGSQEQGMGMDLYKTSKAAQDVWNRADNHFKDTYGFSILDIVINNPVNLTIHFGGEKGKRIRENYSAMIFETIVDGKLKTEKIFKEINEHSTSYTFRSEKGLLSATQFTQPALTLMEKAAFEDLKSKGLIPADATFAGHSLGEYAALASLADVMSIESLVEVVFYRGMTMQVAVPRDELGRSNYGMIAINPGRVAASFSQEALQYVVERVGKRTGWLVEIVNYNVENQQYVAAGDLRALDTVTNVLNFIKLQKIDIIELQKSLSLEEVEGHLFEIIDEASKKSAVKPRPLKLERGFACIPLVGISVPFHSTYLMNGVKPFKSFLKKNIIKENVKVARLAGKYIPNLTAKPFQVTKEYFQDVYDLTGSEPIKEIIDNWEKYEQS

Target ORF information:

RefSeq Version	NM_001179748.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C tetrafunctional fatty acid synthase subunit FAS1 (FAS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179748.1

ORF Insert Sequence:

ATGGACGCTT ACTCCACAAG ACCATTAACC CTATCTCACG GTTCTTTAGA GCACGTGCTT 
CTGGTACCAA CCGCTTCATT TTTCATTGCT TCGCAATTAC AAGAACAATT TAATAAAATT 
TTGCCCGAAC CCACTGAAGG GTTTGCTGCA GATGACGAGC CTACCACACC TGCTGAACTA 
GTGGGGAAAT TCCTTGGCTA CGTATCTTCT CTAGTCGAAC CTTCCAAGGT CGGTCAATTC 
GATCAGGTCT TGAACCTTTG CTTAACAGAA TTTGAAAACT GTTATTTAGA AGGCAATGAC 
ATTCACGCCT TGGCTGCTAA ACTATTACAG GAAAACGACA CAACTTTAGT GAAGACTAAA 
GAACTAATTA AAAATTATAT TACCGCCAGA ATAATGGCTA AGAGACCATT TGACAAAAAA 
TCCAACTCTG CTCTTTTTAG GGCCGTCGGC GAGGGTAACG CACAATTGGT AGCCATTTTC 
GGTGGTCAAG GTAACACCGA CGACTACTTT GAAGAATTGC GTGATCTATA TCAAACTTAT 
CATGTCTTAG TGGGAGATTT AATCAAGTTC TCCGCTGAAA CTTTAAGTGA ACTGATTAGA 
ACTACTTTAG ATGCTGAAAA AGTCTTTACT CAAGGTTTAA ACATATTGGA ATGGTTGGAG 
AACCCTTCAA ATACCCCAGA CAAGGACTAT TTACTTTCCA TTCCAATTTC ATGCCCCTTA 
ATTGGTGTCA TTCAATTGGC TCACTACGTA GTTACTGCCA AGCTTTTGGG TTTCACTCCA 
GGTGAGTTAA GATCTTACTT AAAAGGTGCT ACAGGTCACT CTCAAGGTTT GGTTACTGCT 
GTCGCCATAG CTGAGACGGA TTCCTGGGAA TCCTTCTTCG TCTCCGTAAG AAAAGCAATT 
ACTGTATTAT TCTTCATCGG TGTTCGTTGT TACGAAGCAT ACCCAAACAC TTCCCTACCA 
CCATCCATCT TGGAAGATTC CTTGGAAAAC AATGAAGGTG TTCCATCTCC AATGTTGTCC 
ATTTCCAATC TAACTCAAGA ACAAGTTCAA GACTATGTAA ATAAGACTAA CTCTCATTTG 
CCAGCTGGTA AACAAGTTGA AATTTCTCTA GTCAATGGTG CGAAGAATCT AGTCGTATCG 
GGCCCACCAC AATCATTATA TGGTTTAAAC TTGACTTTAA GAAAGGCCAA GGCCCCATCT 
GGACTGGATC AATCAAGAAT CCCATTCAGC GAAAGAAAAT TGAAGTTCTC CAATAGGTTC 
TTACCTGTTG CATCACCATT CCATTCCCAT CTATTGGTTC CAGCTTCAGA TTTGATTAAC 
AAAGACTTAG TCAAAAACAA TGTCAGCTTT AACGCTAAAG ATATTCAAAT CCCCGTTTAC 
GACACTTTTG ATGGTTCAGA TCTAAGAGTC CTTTCAGGTT CCATTTCCGA GAGAATCGTC 
GACTGCATCA TTAGATTACC TGTCAAATGG GAAACTACTA CACAATTCAA AGCCACCCAC 
ATATTAGACT TTGGTCCAGG TGGAGCTTCC GGTTTAGGTG TTTTAACCCA TCGTAATAAA 
GATGGTACTG GTGTTCGTGT TATCGTTGCC GGTACTCTCG ACATTAACCC AGATGATGAT 
TACGGATTCA AGCAAGAAAT CTTTGATGTT ACTAGTAATG GTTTGAAGAA AAATCCAAAC 
TGGTTGGAAG AATACCATCC AAAATTAATT AAGAACAAAT CAGGCAAAAT TTTTGTCGAA 
ACAAAATTTT CTAAATTAAT CGGTAGACCA CCTTTATTGG TTCCTGGTAT GACACCATGT 
ACTGTTTCTC CAGATTTCGT AGCTGCTACC ACAAATGCTG GTTATACCAT TGAGTTGGCC 
GGTGGTGGTT ACTTTTCCGC AGCAGGTATG ACCGCCGCTA TTGATTCTGT GGTTTCTCAG 
ATAGAAAAGG GTAGTACCTT CGGTATCAAC TTGATCTACG TCAATCCATT TATGTTACAA 
TGGGGTATTC CATTAATCAA GGAACTAAGA AGCAAAGGTT ATCCAATTCA ATTCTTGACC 
ATTGGTGCTG GTGTCCCATC ATTGGAAGTT GCTAGTGAAT ACATAGAGAC ATTAGGTTTG 
AAGTACTTGG GTTTGAAACC AGGTTCCATT GATGCTATTT CGCAAGTTAT AAACATTGCT 
AAAGCACATC CAAACTTCCC AATAGCTTTA CAATGGACCG GTGGTAGAGG TGGTGGTCAT 
CATTCTTTCG AAGATGCCCA CACTCCAATG TTACAAATGT ACTCCAAGAT TAGAAGACAT 
CCAAACATTA TGTTGATATT CGGTTCTGGT TTCGGTTCTG CTGATGACAC TTACCCATAC 
TTAACCGGTG AATGGTCCAC AAAATTCGAT TATCCACCAA TGCCATTCGA TGGTTTCCTA 
TTTGGTTCGA GGGTCATGAT TGCTAAGGAA GTTAAAACTT CTCCTGATGC TAAGAAGTGT 
ATTGCTGCTT GTACTGGTGT TCCTGATGAT AAATGGGAAC AAACCTACAA GAAGCCAACT 
GGTGGTATTG TCACTGTTCG CTCTGAAATG GGTGAACCAA TTCACAAAAT TGCCACTCGT 
GGTGTTATGC TATGGAAGGA ATTCGACGAA ACCATCTTCA ACTTACCAAA GAATAAGTTG 
GTACCAACTT TGGAAGCAAA GAGAGATTAC ATTATCTCAA GATTGAACGC CGATTTCCAA 
AAACCATGGT TTGCTACCGT CAACGGTCAA GCCCGTGACC TAGCCACAAT GACATACGAA 
GAAGTTGCAA AGAGATTGGT GGAATTAATG TTCATCAGAT CTACCAACTC TTGGTTTGAT 
GTCACATGGA GAACCTTTAC TGGTGATTTC CTACGTCGTG TCGAAGAACG TTTCACTAAA 
AGTAAGACAT TGTCTTTAAT CCAATCCTAT TCTCTACTAG ACAAGCCTGA TGAAGCTATT 
GAAAAAGTAT TTAATGCTTA TCCTGCCGCT AGGGAACAGT TCTTGAATGC GCAAGATATT 
GATCACTTTT TGAGCATGTG TCAAAATCCA ATGCAAAAAC CAGTGCCTTT TGTTCCAGTT 
TTGGATCGTA GATTCGAGAT TTTTTTCAAA AAAGATTCGT TATGGCAATC TGAGCACTTG 
GAAGCCGTCG TCGACCAAGA CGTTCAAAGA ACATGTATCC TACATGGACC TGTTGCAGCA 
CAATTCACTA AAGTCATCGA TGAACCAATT AAGAGCATTA TGGATGGTAT TCACGATGGT 
CACATCAAAA AGTTACTACA TCAATATTAC GGTGACGATG AGTCAAAGAT TCCAGCAGTT 
GAGTACTTTG GTGGTGAAAG CCCTGTAGAC GTACAAAGTC AAGTTGATTC TTCCTCTGTA 
TCTGAAGACT CAGCTGTTTT TAAGGCAACA TCCTCTACTG ATGAAGAAAG CTGGTTTAAG 
GCTTTGGCGG GATCCGAAAT TAACTGGAGA CATGCAAGTT TCTTATGTTC CTTTATCACT 
CAAGATAAAA TGTTTGTTTC TAACCCAATT AGAAAAGTTT TCAAGCCAAG CCAAGGAATG 
GTTGTTGAGA TTTCCAACGG CAATACTTCT TCAAAGACTG TTGTCACTCT TTCAGAACCT 
GTTCAAGGTG AATTGAAACC AACTGTTATT TTGAAGTTGT TGAAGGAGAA CATAATCCAA 
ATGGAAATGA TTGAGAACAG AACTATGGAT GGTAAGCCCG TCAGCTTGCC ATTGTTGTAC 
AACTTCAACC CAGATAATGG TTTTGCTCCA ATCTCTGAAG TTATGGAGGA CAGAAACCAA 
AGAATTAAGG AAATGTACTG GAAATTATGG ATTGATGAGC CTTTCAATTT GGACTTTGAC 
CCAAGAGATG TCATTAAGGG CAAAGATTTC GAGATCACCG CTAAAGAAGT TTATGACTTT 
ACACACGCTG TTGGAAACAA TTGTGAAGAC TTCGTTTCTA GACCTGATAG AACGATGTTG 
GCCCCAATGG ACTTTGCTAT TGTTGTCGGA TGGAGAGCCA TCATCAAGGC CATTTTCCCT 
AATACGGTCG ATGGTGACTT ATTGAAGTTG GTTCATTTGT CTAACGGCTA CAAGATGATT 
CCTGGCGCTA AGCCACTGCA AGTTGGTGAT GTTGTTTCAA CTACTGCTGT TATTGAATCT 
GTCGTCAACC AACCTACAGG AAAGATTGTC GATGTGGTAG GTACATTATC GAGAAATGGC 
AAGCCTGTCA TGGAAGTCAC CTCCTCATTC TTCTACAGAG GCAACTATAC TGACTTTGAA 
AACACTTTCC AAAAGACTGT TGAACCTGTT TATCAAATGC ACATCAAAAC TTCTAAAGAT 
ATAGCTGTCT TGCGCTCTAA GGAGTGGTTC CAATTGGACG ATGAAGACTT CGATCTGTTA 
AACAAAACTT TGACTTTCGA AACTGAAACT GAAGTTACTT TCAAGAATGC TAACATCTTC 
TCTTCAGTGA AATGTTTTGG CCCAATTAAA GTTGAATTGC CAACCAAAGA AACCGTGGAG 
ATCGGTATTG TCGATTACGA AGCCGGTGCC TCTCACGGTA ACCCTGTTGT TGATTTCTTG 
AAGAGAAACG GTTCCACATT GGAACAAAAG GTCAATCTAG AAAATCCTAT TCCAATTGCA 
GTACTTGATT CGTACACTCC AAGTACCAAC GAACCATACG CTAGAGTTTC TGGTGATTTG 
AATCCAATTC ACGTTTCACG TCATTTTGCC TCTTACGCAA ACTTGCCAGG TACTATCACG 
CACGGTATGT TTTCTTCTGC TTCCGTCCGT GCTTTGATTG AAAACTGGGC TGCTGACAGT 
GTTTCATCCA GGGTACGTGG CTACACTTGT CAATTTGTTG ACATGGTTTT GCCTAACACT 
GCTTTGAAAA CATCGATTCA ACATGTTGGT ATGATCAATG GTAGAAAATT GATAAAGTTT 
GAAACTAGAA ATGAAGATGA CGTTGTAGTT TTGACTGGTG AAGCCGAAAT TGAACAACCT 
GTTACTACCT TCGTTTTCAC TGGTCAAGGT TCACAAGAAC AAGGTATGGG TATGGACTTA 
TACAAAACTT CTAAAGCTGC TCAAGATGTT TGGAATAGAG CTGACAACCA TTTCAAGGAC 
ACTTATGGTT TCTCTATCTT AGACATTGTC ATTAACAACC CAGTTAACTT AACAATTCAC 
TTCGGTGGTG AAAAGGGTAA GAGGATCAGA GAAAACTATT CTGCTATGAT CTTTGAGACT 
ATCGTGGATG GAAAATTGAA GACTGAAAAA ATTTTCAAGG AAATTAATGA GCACAGTACT 
TCTTACACAT TTAGATCTGA AAAAGGTTTA TTGTCTGCTA CTCAATTTAC ACAACCAGCT 
TTAACTTTGA TGGAAAAAGC TGCTTTCGAA GACTTGAAAT CTAAAGGTTT GATCCCAGCC 
GATGCTACTT TTGCTGGTCA CTCTTTAGGT GAGTATGCTG CTTTGGCCTC TTTGGCTGAT 
GTTATGTCTA TCGAATCTTT AGTTGAAGTT GTGTTCTACA GAGGTATGAC TATGCAAGTT 
GCTGTTCCAA GAGATGAGTT GGGCAGATCC AACTATGGTA TGATTGCCAT TAACCCAGGT 
AGAGTCGCTG CATCATTCTC TCAAGAAGCT TTGCAATATG TTGTTGAGAG AGTTGGTAAG 
AGAACCGGCT GGTTGGTTGA AATCGTCAAC TACAACGTTG AAAACCAACA ATATGTTGCA 
GCTGGTGATC TAAGAGCTTT AGACACCGTT ACCAATGTTC TAAACTTCAT CAAATTACAA 
AAAATTGATA TTATTGAACT ACAAAAGTCC TTATCTTTGG AAGAAGTTGA AGGTCATTTG 
TTTGAGATCA TTGACGAAGC TTCCAAGAAA TCTGCTGTCA AGCCTCGCCC ACTTAAATTG 
GAGAGAGGTT TTGCTTGTAT CCCATTAGTT GGTATTTCTG TTCCTTTCCA TTCCACCTAC 
TTGATGAATG GTGTTAAACC ATTCAAGAGT TTCTTGAAGA AGAATATCAT AAAAGAAAAT 
GTGAAGGTTG CTAGATTGGC CGGAAAGTAC ATTCCAAACT TGACTGCAAA ACCATTCCAG 
GTTACTAAGG AATATTTCCA GGACGTTTAT GATTTGACTG GCTCCGAACC TATCAAGGAA 
ATCATCGACA ACTGGGAAAA GTATGAACAA TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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FAS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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