TOR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TOR1
Entrez Gene ID	853529
Full Name	phosphatidylinositol kinase-related protein kinase TOR1
Synonyms	DRR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181724.1	NP_012600.1	phosphatidylinositol kinase-related protein kinase TOR1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We also show that Tor1p vacuolar localization and TORC1 anabolic functions, including growth promotion and phosphorylation of its direct substrate Sch9, are compromised in s-hhy mutants. Thus, an intact trans-Golgi and late endosome interface is a requisite for efficient Tor1 vacuolar localization and TORC1 function.
Title: Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.

Long non-coding RNA IRT1 transcription is repressed when nutrients are ample and target of rapamycin complex I (TORC1) and protein kinase A (PKA) are active.
Title: Transcription of the mating-type-regulated lncRNA IRT1 is governed by TORC1 and PKA.

Overexpressing SCH9, but not TOR1, allows newly-formed tetraploids to exhibit evolved phenotypes and knocking out SCH9 diminishes the evolved phenotypes. Furthermore, when cells were challenged with conditions causing ancestral cells to evolve aneuploidy, tetraploidy was maintained in the evolved lines. Our results reveal a determinant role for Sch9 during the early stage of polyploid evolution.
Title: Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast.

DNA/RNA helicase Sen1 mutant causes modulation of protein-serine-threonine kinase TOR pathway and autophagy.
Title: Sen1, the homolog of human Senataxin, is critical for cell survival through regulation of redox homeostasis, mitochondrial function, and the TOR pathway in Saccharomyces cerevisiae.

TORC1 can both promote and inhibit gametogenesis. Down-regulation of TORC1 is required to activate IME1. However, complete inactivation of TORC1 inhibits IME1 induction, indicating that an intermediate level of TORC1 signalling is required for entry into sporulation.
Title: Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability.

The following TOR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02762	NM_001181724.1 Latest version!	Saccharomyces cerevisiae S288C phosphatidylinositol kinase-related protein kinase TOR1 (TOR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOR1 ( NM_001181724.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012600.1 Saccharomyces cerevisiae S288C phosphatidylinositol kinase-related protein kinase TOR1 (TOR1), partial mRNA.

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CloneID	OSi02762
Clone ID Related Accession (Same CDS sequence)	NM_001181724.1
Accession Version	NM_001181724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphatidylinositol kinase-related protein kinase TOR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAACCGC ATGAGGAGCA GATTTGGAAG AGTAAACTTT TGAAAGCGGC TAACAACGAT 
ATGGACATGG ATAGAAATGT GCCGTTGGCA CCGAATCTGA ATGTGAATAT GAACATGAAA 
ATGAATGCGA GCAGGAACGG GGATGAATTC GGTCTGACTT CTAGTAGGTT TGATGGAGTG 
GTGATTGGCA GTAATGGGGA TGTAAATTTT AAGCCCATTT TGGAGAAAAT TTTCCGCGAA 
TTAACCAGTG ATTACAAGGA GGAACGAAAA TTGGCCAGTA TTTCATTATT TGATCTACTA 
GTATCCTTGG AACATGAATT GTCGATAGAA GAGTTCCAAG CAGTTTCAAA TGACATAAAC 
AATAAGATTT TGGAGCTGGT CCATACAAAA AAAACGAGCA CTAGGGTAGG GGCTGTTCTA 
TCCATAGACA CTTTGATTTC ATTCTACGCA TATACTGAAA GGTTGCCTAA CGAAACTTCA 
CGACTGGCTG GTTACCTTCG AGGGCTAATA CCTTCTAATG ATGTAGAGGT CATGAGACTC 
GCTGCAAAGA CTCTGGGCAA GTTAGCCGTT CCAGGAGGTA CATATACCTC TGATTTCGTG 
GAATTTGAGA TAAAGTCTTG CTTAGAATGG CTTACTGCCT CCACGGAAAA GAATTCATTC 
TCGAGTTCGA AGCCAGACCA TGCTAAACAT GCTGCGCTTC TGATTATAAC AGCGTTGGCA 
GAGAATTGTC CTTATTTACT CTACCAATAC TTGAATTCCA TACTAGATAA CATTTGGAGA 
GCACTAAGAG ACCCACATTT GGTGATCAGA ATTGATGCGT CCATTACATT GGCCAAATGT 
CTTTCCACCC TACGAAATAG GGATCCTCAG TTAACTAGCC AGTGGGTGCA GAGATTGGCT 
ACAAGTTGTG AATACGGATT TCAAGTAAAC ACATTAGAAT GCATCCATGC AAGTTTGTTG 
GTTTATAAGG AAATCTTGTT TTTGAAGGAT CCCTTTTTGA ATCAAGTGTT CGACCAAATG 
TGTCTAAATT GCATAGCTTA TGAAAATCAT AAAGCGAAAA TGATTAGAGA AAAGATTTAC 
CAGATTGTTC CCCTATTAGC ATCGTTCAAT CCTCAATTAT TTGCTGGCAA ATATTTGCAC 
CAAATTATGG ACAACTATTT AGAGATTTTA ACCAATGCTC CAGCAAATAA AATACCACAT 
CTCAAAGATG ACAAACCACA GATTTTAATA TCGATTGGTG ATATTGCATA TGAAGTCGGG 
CCCGATATCG CACCTTATGT GAAACAAATT CTTGATTATA TTGAACATGA TTTACAGACG 
AAATTCAAAT TCAGAAAGAA ATTTGAAAAT GAAATTTTCT ACTGCATCGG AAGATTGGCA 
GTTCCCTTGG GCCCCGTTCT AGGTAAATTA TTAAACAGAA ATATACTGGA CCTGATGTTC 
AAATGCCCTC TTTCCGACTA TATGCAGGAA ACGTTTCAAA TTCTGACTGA GAGAATACCA 
TCACTAGGCC CCAAAATAAA TGACGAGTTG CTTAACCTAG TCTGTTCAAC CTTATCTGGA 
ACACCATTTA TCCAGCCAGG GTCACCAATG GAGATACCAT CGTTTTCGAG AGAAAGAGCA 
AGAGAATGGA GAAATAAAAA CATCCTACAG AAAACTGGTG AAAGTAACGA TGATAATAAT 
GATATAAAAA TCATTATACA AGCTTTTAGA ATGTTAAAAA ATATCAAAAG CAGATTTTCG 
TTGGTGGAAT TCGTGAGAAT TGTTGCACTT TCTTACATTG AGCATACAGA TCCCAGAGTA 
AGGAAACTAG CTGCGTTGAC ATCTTGTGAA ATTTACGTCA AGGATAACAT CTGCAAACAA 
ACATCACTAC ACTCTCTGAA CACTGTATCT GAAGTGTTAT CAAAGCTTCT AGCCATTACG 
ATTGCGGACC CTTTACAAGA TATCCGTTTA GAAGTTTTAA AGAATCTTAA TCCATGTTTC 
GATCCCCAGT TGGCACAACC AGATAATTTG AGACTCTTGT TTACTGCACT GCACGATGAG 
TCGTTCAATA TTCAGTCAGT AGCAATGGAG CTTGTCGGTA GGTTGTCTTC CGTAAACCCT 
GCATACGTCA TCCCATCGAT AAGAAAAATA CTACTGGAAC TGCTAACAAA ATTAAAATTC 
TCAACTTCTT CTCGAGAAAA GGAAGAAACT GCCAGTTTGT TATGTACTCT TATCAGGTCG 
AGTAAAGATG TTGCGAAACC TTATATCGAA CCTCTTTTAA ATGTTCTTTT ACCAAAATTC 
CAAGATACCT CTTCAACGGT TGCATCAACT GCACTGAGAA CTATAGGTGA GCTATCTGTT 
GTAGGGGGCG AAGATATGAA GATATATCTT AAGGATTTGT TTCCTTTAAT TATCAAAACA 
TTTCAGGATC AATCAAACTC TTTCAAGAGA GAAGCTGCAC TTAAGGCCCT TGGTCAACTT 
GCAGCCTCAT CTGGTTACGT GATAGATCCT TTACTCGACT ATCCCGAATT ATTGGGTATA 
TTGGTGAATA TATTGAAGAC AGAAAACTCT CAAAATATTA GGAGACAAAC AGTCACTTTG 
ATAGGTATAC TGGGAGCTAT CGACCCATAT CGCCAAAAAG AACGTGAGGT TACCTCTACT 
ACCGATATAT CTACAGAACA GAACGCCCCG CCTATCGACA TTGCTCTTCT CATGCAGGGC 
ATGTCTCCTT CGAATGATGA GTATTATACC ACTGTTGTCA TTCACTGCCT GCTAAAAATC 
CTAAAAGATC CATCCCTATC ATCTTACCAC ACTGCCGTGA TCCAAGCGAT TATGCATATT 
TTTCAAACCC TTGGTCTAAA ATGTGTTTCA TTCTTGGACC AGATCATCCC AACTATTTTG 
GACGTAATGC GTACATGCTC TCAGTCACTA TTAGAATTTT ACTTCCAACA GCTTTGCTCT 
TTGATTATTA TCGTAAGGCA ACACATAAGA CCTCATGTCG ATTCTATATT CCAGGCTATC 
AAAGATTTTT CTTCGGTTGC TAAGCTACAA ATAACGCTTG TAAGTGTTAT TGAAGCAATA 
TCAAAGGCTC TGGAGGGTGA ATTCAAAAGA TTGGTCCCTC TTACTCTGAC CTTGTTCCTT 
GTAATTTTGG AGAATGACAA GTCTAGTGAC AAGGTCCTCT CCAGAAGGGT ATTGAGACTG 
TTAGAATCGT TTGGTCCTAA CTTAGAAGGT TATTCGCATT TGATTACACC CAAGATAGTT 
CAAATGGCAG AATTCACCAG CGGGAACCTA CAAAGGTCTG CAATAATTAC TATTGGCAAA 
CTGGCCAAGG ATGTTGACCT TTTTGAGATG TCCTCAAGAA TTGTTCACTC TTTACTTAGG 
GTACTAAGTT CAACAACGAG TGACGAACTC TCAAAAGTCA TTATGAATAC TTTAAGTCTA 
CTGCTAATAC AAATGGGCAC ATCCTTTGCT ATCTTCATCC CTGTCATTAA TGAAGTTTTA 
ATGAAGAAAC ATATTCAACA CACAATATAT GATGACTTGA CAAACAGAAT ATTAAACAAT 
GATGTTTTAC CCACAAAAAT TCTTGAAGCA AATACAACGG ATTATAAGCC CGCGGAACAA 
ATGGAGGCAG CAGATGCTGG GGTCGCAAAA TTACCTATAA ACCAATCAGT TTTGAAAAGT 
GCATGGAATT CTAGCCAACA AAGAACTAAA GAAGATTGGC AGGAATGGAG CAAACGTCTA 
TCCATTCAAT TATTAAAAGA GTCACCCTCC CATGCTCTAA GAGCTTGTTC AAATCTTGCA 
AGCATGTATT ATCCACTAGC CAAAGAACTT TTTAATACCG CATTCGCATG TGTTTGGACC 
GAACTTTATA GCCAATATCA AGAAGATTTA ATTGGGTCAT TATGTATAGC CTTATCTTCT 
CCCTTAAATC CACCAGAAAT ACATCAAACA TTGTTAAACC TGGTAGAATT TATGGAACAC 
GATGACAAGG CATTACCAAT ACCAACTCAA AGCCTGGGCG AGTATGCTGA AAGATGTCAC 
GCCTATGCCA AAGCGCTACA TTATAAAGAG ATTAAATTTA TTAAAGAGCC TGAGAACTCA 
ACTATTGAAT CATTGATCAG CATTAACAAC CAGCTGAATC AAACGGATGC TGCAATTGGT 
ATATTAAAGC ATGCCCAACA ACATCATTCA CTTCAATTAA AGGAGACATG GTTTGAAAAA 
TTAGAGCGTT GGGAAGATGC ACTACATGCT TATAATGAAC GTGAAAAGGC AGGTGATACT 
TCCGTGAGCG TTACACTCGG TAAGATGAGA TCCCTTCATG CCCTTGGCGA ATGGGAACAG 
TTGTCGCAAT TGGCAGCTAG AAAGTGGAAA GTTTCGAAGC TACAAACTAA GAAGCTAATA 
GCTCCCTTGG CAGCTGGTGC TGCGTGGGGG TTGGGAGAGT GGGATATGCT TGAGCAATAT 
ATCAGCGTTA TGAAACCTAA ATCTCCAGAT AAGGAATTTT TTGATGCAAT TTTATACTTG 
CACAAGAATG ATTACGACAA TGCTAGTAAG CATATATTAA ACGCCAGAGA TTTGCTTGTG 
ACTGAAATTT CCGCGTTGAT CAATGAAAGT TATAATAGAG CATATAGCGT TATTGTTAGA 
ACTCAAATAA TAACAGAGTT TGAGGAAATC ATCAAGTATA AACAATTGCC ACCTAATTCC 
GAGAAAAAAC TTCACTATCA AAATCTTTGG ACAAAAAGAC TGCTGGGCTG CCAAAAAAAT 
GTCGATTTAT GGCAAAGAGT GCTTAGAGTA AGATCATTGG TAATAAAGCC CAAGCAAGAC 
CTGCAAATAT GGATAAAATT TGCAAATTTG TGCAGAAAAT CTGGTAGAAT GAGGCTAGCA 
AATAAGGCAT TGAATATGCT ACTAGAAGGA GGCAACGATC CTAGTTTACC AAATACGTTC 
AAAGCTCCTC CCCCAGTTGT TTACGCGCAA CTAAAATATA TTTGGGCTAC AGGAGCTTAT 
AAAGAAGCAT TAAACCACTT GATAGGATTT ACATCCAGGT TAGCGCATGA TCTTGGTTTG 
GATCCGAATA ATATGATCGC GCAAAGTGTC AAACTCTCAA GTGCAAGTAC TGCTCCGTAT 
GTTGAGGAAT ACACAAAATT ATTAGCTCGA TGTTTTTTAA AGCAAGGTGA GTGGAGAATA 
GCAACACAAC CGAACTGGAG AAACACAAAT CCGGATGCAA TTCTTGGTTC TTATCTATTG 
GCTACACATT TCGATAAAAA TTGGTACAAG GCATGGCATA ATTGGGCCTT AGCTAATTTT 
GAAGTAATAT CCATGGTTCA GGAAGAGACT AAGCTCAACG GAGGTAAGAA TGATGATGAT 
GATGACACGG CAGTTAATAA TGATAATGTG CGGATTGACG GTAGTATCCT AGGAAGTGGT 
TCTTTGACTA TTAATGGCAA CAGATACCCG CTAGAGCTTA TTCAAAGACA TGTTGTTCCA 
GCGATCAAGG GCTTTTTTCA TTCAATATCT CTATTAGAAA CAAGTTGTTT GCAAGACACG 
TTGAGGTTAT TGACTCTTTT ATTTAACTTT GGTGGTATTA AAGAAGTCTC ACAAGCCATG 
TATGAAGGCT TCAATTTGAT GAAAATAGAG AACTGGCTTG AAGTCTTACC ACAGTTGATC 
TCTCGTATAC ATCAGCCAGA TCCTACGGTG AGTAATTCCC TTTTGTCGTT GCTTTCTGAT 
TTAGGGAAAG CTCATCCACA AGCTCTCGTG TATCCTTTAA CTGTCGCGAT CAAGTCTGAA 
TCTGTTTCAA GACAAAAAGC GGCTCTTTCA ATAATAGAGA AAATTAGGAT TCATAGTCCA 
GTCCTGGTAA ACCAGGCAGA ATTAGTTAGT CACGAGTTGA TCAGAGTAGC CGTTCTATGG 
CACGAATTAT GGTATGAAGG ACTGGAAGAT GCGAGCCGCC AATTTTTCGT TGAACATAAC 
ATAGAAAAAA TGTTTTCTAC TTTAGAACCT TTACATAAAC ACTTAGGCAA TGAGCCTCAA 
ACGTTAAGTG AGGTATCGTT TCAGAAATCA TTTGGTAGAG ATTTGAACGA TGCCTACGAA 
TGGTTGAATA ACTACAAAAA GTCAAAAGAC ATCAATAATT TGAACCAAGC TTGGGATATT 
TATTATAACG TCTTCAGAAA AATAACACGT CAAATACCAC AGTTACAAAC CTTAGACTTA 
CAGCATGTTT CTCCCCAGCT TCTGGCTACT CATGATCTCG AATTGGCTGT TCCTGGGACA 
TATTTCCCAG GAAAACCTAC CATTAGAATA GCGAAGTTTG AGCCATTATT TTCTGTGATC 
TCTTCGAAGC AAAGGCCAAG AAAATTCTCC ATCAAGGGTA GCGACGGTAA AGATTATAAA 
TACGTTTTAA AGGGACATGA AGATATAAGA CAAGATAGCC TTGTTATGCA ATTATTTGGT 
CTAGTTAACA CTTTGTTGAA GAATGATTCA GAGTGTTTCA AGAGACATTT GGATATCCAA 
CAATACCCGG CTATTCCATT GTCGCCTAAA TCTGGTTTAC TAGGATGGGT ACCAAATAGT 
GACACATTCC ACGTTTTGAT CAGAGAACAC CGTGATGCCA AAAAAATTCC GTTGAACATT 
GAACATTGGG TTATGTTACA AATGGCCCCC GATTATGAGA ATTTGACTCT TTTACAAAAA 
ATTGAAGTAT TCACGTACGC TTTAGATAAT ACAAAAGGCC AAGACCTTTA TAAAATATTA 
TGGTTAAAGA GTAGGTCGTC AGAGACATGG CTAGAACGTA GAACAACTTA TACGAGATCT 
TTAGCAGTTA TGTCCATGAC TGGTTATATT CTGGGACTAG GTGATCGCCA TCCAAGCAAC 
CTGATGCTAG ATAGAATCAC CGGTAAAGTT ATCCACATTG ATTTCGGCGA TTGTTTTGAA 
GCTGCCATCT TAAGAGAAAA GTATCCAGAA AAAGTGCCAT TTAGACTAAC TAGGATGTTA 
ACATACGCAA TGGAAGTTAG TGGAATTGAA GGCAGTTTCC GAATTACTTG TGAAAATGTC 
ATGAGAGTCT TAAGAGATAA TAAAGAATCA TTAATGGCGA TCTTGGAAGC TTTTGCGCTT 
GATCCTTTGA TCCATTGGGG ATTTGATTTA CCGCCACAAA AACTTACTGA GCAAACTGGA 
ATTCCTTTGC CGTTGATTAA TCCTAGTGAA TTATTAAGGA AGGGGGCAAT TACTGTCGAA 
GAAGCGGCAA ATATGGAAGC AGAACAACAA AATGAGACCA AAAACGCCAG AGCAATGCTT 
GTTTTGAGAC GTATTACAGA TAAATTAACG GGCAATGATA TCAAGAGGTT CAATGAATTA 
GACGTCCCTG AGCAGGTTGA TAAACTGATC CAACAAGCCA CTTCTATTGA AAGGTTATGT 
CAACATTATA TTGGATGGTG CCCATTCTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012600.1
CDS	1..7413
Translation	MEPHEEQIWKSKLLKAANNDMDMDRNVPLAPNLNVNMNMKMNASRNGDEFGLTSSRFDGVVIGSNGDVNFKPILEKIFRELTSDYKEERKLASISLFDLLVSLEHELSIEEFQAVSNDINNKILELVHTKKTSTRVGAVLSIDTLISFYAYTERLPNETSRLAGYLRGLIPSNDVEVMRLAAKTLGKLAVPGGTYTSDFVEFEIKSCLEWLTASTEKNSFSSSKPDHAKHAALLIITALAENCPYLLYQYLNSILDNIWRALRDPHLVIRIDASITLAKCLSTLRNRDPQLTSQWVQRLATSCEYGFQVNTLECIHASLLVYKEILFLKDPFLNQVFDQMCLNCIAYENHKAKMIREKIYQIVPLLASFNPQLFAGKYLHQIMDNYLEILTNAPANKIPHLKDDKPQILISIGDIAYEVGPDIAPYVKQILDYIEHDLQTKFKFRKKFENEIFYCIGRLAVPLGPVLGKLLNRNILDLMFKCPLSDYMQETFQILTERIPSLGPKINDELLNLVCSTLSGTPFIQPGSPMEIPSFSRERAREWRNKNILQKTGESNDDNNDIKIIIQAFRMLKNIKSRFSLVEFVRIVALSYIEHTDPRVRKLAALTSCEIYVKDNICKQTSLHSLNTVSEVLSKLLAITIADPLQDIRLEVLKNLNPCFDPQLAQPDNLRLLFTALHDESFNIQSVAMELVGRLSSVNPAYVIPSIRKILLELLTKLKFSTSSREKEETASLLCTLIRSSKDVAKPYIEPLLNVLLPKFQDTSSTVASTALRTIGELSVVGGEDMKIYLKDLFPLIIKTFQDQSNSFKREAALKALGQLAASSGYVIDPLLDYPELLGILVNILKTENSQNIRRQTVTLIGILGAIDPYRQKEREVTSTTDISTEQNAPPIDIALLMQGMSPSNDEYYTTVVIHCLLKILKDPSLSSYHTAVIQAIMHIFQTLGLKCVSFLDQIIPTILDVMRTCSQSLLEFYFQQLCSLIIIVRQHIRPHVDSIFQAIKDFSSVAKLQITLVSVIEAISKALEGEFKRLVPLTLTLFLVILENDKSSDKVLSRRVLRLLESFGPNLEGYSHLITPKIVQMAEFTSGNLQRSAIITIGKLAKDVDLFEMSSRIVHSLLRVLSSTTSDELSKVIMNTLSLLLIQMGTSFAIFIPVINEVLMKKHIQHTIYDDLTNRILNNDVLPTKILEANTTDYKPAEQMEAADAGVAKLPINQSVLKSAWNSSQQRTKEDWQEWSKRLSIQLLKESPSHALRACSNLASMYYPLAKELFNTAFACVWTELYSQYQEDLIGSLCIALSSPLNPPEIHQTLLNLVEFMEHDDKALPIPTQSLGEYAERCHAYAKALHYKEIKFIKEPENSTIESLISINNQLNQTDAAIGILKHAQQHHSLQLKETWFEKLERWEDALHAYNEREKAGDTSVSVTLGKMRSLHALGEWEQLSQLAARKWKVSKLQTKKLIAPLAAGAAWGLGEWDMLEQYISVMKPKSPDKEFFDAILYLHKNDYDNASKHILNARDLLVTEISALINESYNRAYSVIVRTQIITEFEEIIKYKQLPPNSEKKLHYQNLWTKRLLGCQKNVDLWQRVLRVRSLVIKPKQDLQIWIKFANLCRKSGRMRLANKALNMLLEGGNDPSLPNTFKAPPPVVYAQLKYIWATGAYKEALNHLIGFTSRLAHDLGLDPNNMIAQSVKLSSASTAPYVEEYTKLLARCFLKQGEWRIATQPNWRNTNPDAILGSYLLATHFDKNWYKAWHNWALANFEVISMVQEETKLNGGKNDDDDDTAVNNDNVRIDGSILGSGSLTINGNRYPLELIQRHVVPAIKGFFHSISLLETSCLQDTLRLLTLLFNFGGIKEVSQAMYEGFNLMKIENWLEVLPQLISRIHQPDPTVSNSLLSLLSDLGKAHPQALVYPLTVAIKSESVSRQKAALSIIEKIRIHSPVLVNQAELVSHELIRVAVLWHELWYEGLEDASRQFFVEHNIEKMFSTLEPLHKHLGNEPQTLSEVSFQKSFGRDLNDAYEWLNNYKKSKDINNLNQAWDIYYNVFRKITRQIPQLQTLDLQHVSPQLLATHDLELAVPGTYFPGKPTIRIAKFEPLFSVISSKQRPRKFSIKGSDGKDYKYVLKGHEDIRQDSLVMQLFGLVNTLLKNDSECFKRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREHRDAKKIPLNIEHWVMLQMAPDYENLTLLQKIEVFTYALDNTKGQDLYKILWLKSRSSETWLERRTTYTRSLAVMSMTGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILREKYPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMRVLRDNKESLMAILEAFALDPLIHWGFDLPPQKLTEQTGIPLPLINPSELLRKGAITVEEAANMEAEQQNETKNARAMLVLRRITDKLTGNDIKRFNELDVPEQVDKLIQQATSIERLCQHYIGWCPFW

Target ORF information:

RefSeq Version	NM_001181724.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphatidylinositol kinase-related protein kinase TOR1 (TOR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181724.1

ORF Insert Sequence:

ATGGAACCGC ATGAGGAGCA GATTTGGAAG AGTAAACTTT TGAAAGCGGC TAACAACGAT 
ATGGACATGG ATAGAAATGT GCCGTTGGCA CCGAATCTGA ATGTGAATAT GAACATGAAA 
ATGAATGCGA GCAGGAACGG GGATGAATTC GGTCTGACTT CTAGTAGGTT TGATGGAGTG 
GTGATTGGCA GTAATGGGGA TGTAAATTTT AAGCCCATTT TGGAGAAAAT TTTCCGCGAA 
TTAACCAGTG ATTACAAGGA GGAACGAAAA TTGGCCAGTA TTTCATTATT TGATCTACTA 
GTATCCTTGG AACATGAATT GTCGATAGAA GAGTTCCAAG CAGTTTCAAA TGACATAAAC 
AATAAGATTT TGGAGCTGGT CCATACAAAA AAAACGAGCA CTAGGGTAGG GGCTGTTCTA 
TCCATAGACA CTTTGATTTC ATTCTACGCA TATACTGAAA GGTTGCCTAA CGAAACTTCA 
CGACTGGCTG GTTACCTTCG AGGGCTAATA CCTTCTAATG ATGTAGAGGT CATGAGACTC 
GCTGCAAAGA CTCTGGGCAA GTTAGCCGTT CCAGGAGGTA CATATACCTC TGATTTCGTG 
GAATTTGAGA TAAAGTCTTG CTTAGAATGG CTTACTGCCT CCACGGAAAA GAATTCATTC 
TCGAGTTCGA AGCCAGACCA TGCTAAACAT GCTGCGCTTC TGATTATAAC AGCGTTGGCA 
GAGAATTGTC CTTATTTACT CTACCAATAC TTGAATTCCA TACTAGATAA CATTTGGAGA 
GCACTAAGAG ACCCACATTT GGTGATCAGA ATTGATGCGT CCATTACATT GGCCAAATGT 
CTTTCCACCC TACGAAATAG GGATCCTCAG TTAACTAGCC AGTGGGTGCA GAGATTGGCT 
ACAAGTTGTG AATACGGATT TCAAGTAAAC ACATTAGAAT GCATCCATGC AAGTTTGTTG 
GTTTATAAGG AAATCTTGTT TTTGAAGGAT CCCTTTTTGA ATCAAGTGTT CGACCAAATG 
TGTCTAAATT GCATAGCTTA TGAAAATCAT AAAGCGAAAA TGATTAGAGA AAAGATTTAC 
CAGATTGTTC CCCTATTAGC ATCGTTCAAT CCTCAATTAT TTGCTGGCAA ATATTTGCAC 
CAAATTATGG ACAACTATTT AGAGATTTTA ACCAATGCTC CAGCAAATAA AATACCACAT 
CTCAAAGATG ACAAACCACA GATTTTAATA TCGATTGGTG ATATTGCATA TGAAGTCGGG 
CCCGATATCG CACCTTATGT GAAACAAATT CTTGATTATA TTGAACATGA TTTACAGACG 
AAATTCAAAT TCAGAAAGAA ATTTGAAAAT GAAATTTTCT ACTGCATCGG AAGATTGGCA 
GTTCCCTTGG GCCCCGTTCT AGGTAAATTA TTAAACAGAA ATATACTGGA CCTGATGTTC 
AAATGCCCTC TTTCCGACTA TATGCAGGAA ACGTTTCAAA TTCTGACTGA GAGAATACCA 
TCACTAGGCC CCAAAATAAA TGACGAGTTG CTTAACCTAG TCTGTTCAAC CTTATCTGGA 
ACACCATTTA TCCAGCCAGG GTCACCAATG GAGATACCAT CGTTTTCGAG AGAAAGAGCA 
AGAGAATGGA GAAATAAAAA CATCCTACAG AAAACTGGTG AAAGTAACGA TGATAATAAT 
GATATAAAAA TCATTATACA AGCTTTTAGA ATGTTAAAAA ATATCAAAAG CAGATTTTCG 
TTGGTGGAAT TCGTGAGAAT TGTTGCACTT TCTTACATTG AGCATACAGA TCCCAGAGTA 
AGGAAACTAG CTGCGTTGAC ATCTTGTGAA ATTTACGTCA AGGATAACAT CTGCAAACAA 
ACATCACTAC ACTCTCTGAA CACTGTATCT GAAGTGTTAT CAAAGCTTCT AGCCATTACG 
ATTGCGGACC CTTTACAAGA TATCCGTTTA GAAGTTTTAA AGAATCTTAA TCCATGTTTC 
GATCCCCAGT TGGCACAACC AGATAATTTG AGACTCTTGT TTACTGCACT GCACGATGAG 
TCGTTCAATA TTCAGTCAGT AGCAATGGAG CTTGTCGGTA GGTTGTCTTC CGTAAACCCT 
GCATACGTCA TCCCATCGAT AAGAAAAATA CTACTGGAAC TGCTAACAAA ATTAAAATTC 
TCAACTTCTT CTCGAGAAAA GGAAGAAACT GCCAGTTTGT TATGTACTCT TATCAGGTCG 
AGTAAAGATG TTGCGAAACC TTATATCGAA CCTCTTTTAA ATGTTCTTTT ACCAAAATTC 
CAAGATACCT CTTCAACGGT TGCATCAACT GCACTGAGAA CTATAGGTGA GCTATCTGTT 
GTAGGGGGCG AAGATATGAA GATATATCTT AAGGATTTGT TTCCTTTAAT TATCAAAACA 
TTTCAGGATC AATCAAACTC TTTCAAGAGA GAAGCTGCAC TTAAGGCCCT TGGTCAACTT 
GCAGCCTCAT CTGGTTACGT GATAGATCCT TTACTCGACT ATCCCGAATT ATTGGGTATA 
TTGGTGAATA TATTGAAGAC AGAAAACTCT CAAAATATTA GGAGACAAAC AGTCACTTTG 
ATAGGTATAC TGGGAGCTAT CGACCCATAT CGCCAAAAAG AACGTGAGGT TACCTCTACT 
ACCGATATAT CTACAGAACA GAACGCCCCG CCTATCGACA TTGCTCTTCT CATGCAGGGC 
ATGTCTCCTT CGAATGATGA GTATTATACC ACTGTTGTCA TTCACTGCCT GCTAAAAATC 
CTAAAAGATC CATCCCTATC ATCTTACCAC ACTGCCGTGA TCCAAGCGAT TATGCATATT 
TTTCAAACCC TTGGTCTAAA ATGTGTTTCA TTCTTGGACC AGATCATCCC AACTATTTTG 
GACGTAATGC GTACATGCTC TCAGTCACTA TTAGAATTTT ACTTCCAACA GCTTTGCTCT 
TTGATTATTA TCGTAAGGCA ACACATAAGA CCTCATGTCG ATTCTATATT CCAGGCTATC 
AAAGATTTTT CTTCGGTTGC TAAGCTACAA ATAACGCTTG TAAGTGTTAT TGAAGCAATA 
TCAAAGGCTC TGGAGGGTGA ATTCAAAAGA TTGGTCCCTC TTACTCTGAC CTTGTTCCTT 
GTAATTTTGG AGAATGACAA GTCTAGTGAC AAGGTCCTCT CCAGAAGGGT ATTGAGACTG 
TTAGAATCGT TTGGTCCTAA CTTAGAAGGT TATTCGCATT TGATTACACC CAAGATAGTT 
CAAATGGCAG AATTCACCAG CGGGAACCTA CAAAGGTCTG CAATAATTAC TATTGGCAAA 
CTGGCCAAGG ATGTTGACCT TTTTGAGATG TCCTCAAGAA TTGTTCACTC TTTACTTAGG 
GTACTAAGTT CAACAACGAG TGACGAACTC TCAAAAGTCA TTATGAATAC TTTAAGTCTA 
CTGCTAATAC AAATGGGCAC ATCCTTTGCT ATCTTCATCC CTGTCATTAA TGAAGTTTTA 
ATGAAGAAAC ATATTCAACA CACAATATAT GATGACTTGA CAAACAGAAT ATTAAACAAT 
GATGTTTTAC CCACAAAAAT TCTTGAAGCA AATACAACGG ATTATAAGCC CGCGGAACAA 
ATGGAGGCAG CAGATGCTGG GGTCGCAAAA TTACCTATAA ACCAATCAGT TTTGAAAAGT 
GCATGGAATT CTAGCCAACA AAGAACTAAA GAAGATTGGC AGGAATGGAG CAAACGTCTA 
TCCATTCAAT TATTAAAAGA GTCACCCTCC CATGCTCTAA GAGCTTGTTC AAATCTTGCA 
AGCATGTATT ATCCACTAGC CAAAGAACTT TTTAATACCG CATTCGCATG TGTTTGGACC 
GAACTTTATA GCCAATATCA AGAAGATTTA ATTGGGTCAT TATGTATAGC CTTATCTTCT 
CCCTTAAATC CACCAGAAAT ACATCAAACA TTGTTAAACC TGGTAGAATT TATGGAACAC 
GATGACAAGG CATTACCAAT ACCAACTCAA AGCCTGGGCG AGTATGCTGA AAGATGTCAC 
GCCTATGCCA AAGCGCTACA TTATAAAGAG ATTAAATTTA TTAAAGAGCC TGAGAACTCA 
ACTATTGAAT CATTGATCAG CATTAACAAC CAGCTGAATC AAACGGATGC TGCAATTGGT 
ATATTAAAGC ATGCCCAACA ACATCATTCA CTTCAATTAA AGGAGACATG GTTTGAAAAA 
TTAGAGCGTT GGGAAGATGC ACTACATGCT TATAATGAAC GTGAAAAGGC AGGTGATACT 
TCCGTGAGCG TTACACTCGG TAAGATGAGA TCCCTTCATG CCCTTGGCGA ATGGGAACAG 
TTGTCGCAAT TGGCAGCTAG AAAGTGGAAA GTTTCGAAGC TACAAACTAA GAAGCTAATA 
GCTCCCTTGG CAGCTGGTGC TGCGTGGGGG TTGGGAGAGT GGGATATGCT TGAGCAATAT 
ATCAGCGTTA TGAAACCTAA ATCTCCAGAT AAGGAATTTT TTGATGCAAT TTTATACTTG 
CACAAGAATG ATTACGACAA TGCTAGTAAG CATATATTAA ACGCCAGAGA TTTGCTTGTG 
ACTGAAATTT CCGCGTTGAT CAATGAAAGT TATAATAGAG CATATAGCGT TATTGTTAGA 
ACTCAAATAA TAACAGAGTT TGAGGAAATC ATCAAGTATA AACAATTGCC ACCTAATTCC 
GAGAAAAAAC TTCACTATCA AAATCTTTGG ACAAAAAGAC TGCTGGGCTG CCAAAAAAAT 
GTCGATTTAT GGCAAAGAGT GCTTAGAGTA AGATCATTGG TAATAAAGCC CAAGCAAGAC 
CTGCAAATAT GGATAAAATT TGCAAATTTG TGCAGAAAAT CTGGTAGAAT GAGGCTAGCA 
AATAAGGCAT TGAATATGCT ACTAGAAGGA GGCAACGATC CTAGTTTACC AAATACGTTC 
AAAGCTCCTC CCCCAGTTGT TTACGCGCAA CTAAAATATA TTTGGGCTAC AGGAGCTTAT 
AAAGAAGCAT TAAACCACTT GATAGGATTT ACATCCAGGT TAGCGCATGA TCTTGGTTTG 
GATCCGAATA ATATGATCGC GCAAAGTGTC AAACTCTCAA GTGCAAGTAC TGCTCCGTAT 
GTTGAGGAAT ACACAAAATT ATTAGCTCGA TGTTTTTTAA AGCAAGGTGA GTGGAGAATA 
GCAACACAAC CGAACTGGAG AAACACAAAT CCGGATGCAA TTCTTGGTTC TTATCTATTG 
GCTACACATT TCGATAAAAA TTGGTACAAG GCATGGCATA ATTGGGCCTT AGCTAATTTT 
GAAGTAATAT CCATGGTTCA GGAAGAGACT AAGCTCAACG GAGGTAAGAA TGATGATGAT 
GATGACACGG CAGTTAATAA TGATAATGTG CGGATTGACG GTAGTATCCT AGGAAGTGGT 
TCTTTGACTA TTAATGGCAA CAGATACCCG CTAGAGCTTA TTCAAAGACA TGTTGTTCCA 
GCGATCAAGG GCTTTTTTCA TTCAATATCT CTATTAGAAA CAAGTTGTTT GCAAGACACG 
TTGAGGTTAT TGACTCTTTT ATTTAACTTT GGTGGTATTA AAGAAGTCTC ACAAGCCATG 
TATGAAGGCT TCAATTTGAT GAAAATAGAG AACTGGCTTG AAGTCTTACC ACAGTTGATC 
TCTCGTATAC ATCAGCCAGA TCCTACGGTG AGTAATTCCC TTTTGTCGTT GCTTTCTGAT 
TTAGGGAAAG CTCATCCACA AGCTCTCGTG TATCCTTTAA CTGTCGCGAT CAAGTCTGAA 
TCTGTTTCAA GACAAAAAGC GGCTCTTTCA ATAATAGAGA AAATTAGGAT TCATAGTCCA 
GTCCTGGTAA ACCAGGCAGA ATTAGTTAGT CACGAGTTGA TCAGAGTAGC CGTTCTATGG 
CACGAATTAT GGTATGAAGG ACTGGAAGAT GCGAGCCGCC AATTTTTCGT TGAACATAAC 
ATAGAAAAAA TGTTTTCTAC TTTAGAACCT TTACATAAAC ACTTAGGCAA TGAGCCTCAA 
ACGTTAAGTG AGGTATCGTT TCAGAAATCA TTTGGTAGAG ATTTGAACGA TGCCTACGAA 
TGGTTGAATA ACTACAAAAA GTCAAAAGAC ATCAATAATT TGAACCAAGC TTGGGATATT 
TATTATAACG TCTTCAGAAA AATAACACGT CAAATACCAC AGTTACAAAC CTTAGACTTA 
CAGCATGTTT CTCCCCAGCT TCTGGCTACT CATGATCTCG AATTGGCTGT TCCTGGGACA 
TATTTCCCAG GAAAACCTAC CATTAGAATA GCGAAGTTTG AGCCATTATT TTCTGTGATC 
TCTTCGAAGC AAAGGCCAAG AAAATTCTCC ATCAAGGGTA GCGACGGTAA AGATTATAAA 
TACGTTTTAA AGGGACATGA AGATATAAGA CAAGATAGCC TTGTTATGCA ATTATTTGGT 
CTAGTTAACA CTTTGTTGAA GAATGATTCA GAGTGTTTCA AGAGACATTT GGATATCCAA 
CAATACCCGG CTATTCCATT GTCGCCTAAA TCTGGTTTAC TAGGATGGGT ACCAAATAGT 
GACACATTCC ACGTTTTGAT CAGAGAACAC CGTGATGCCA AAAAAATTCC GTTGAACATT 
GAACATTGGG TTATGTTACA AATGGCCCCC GATTATGAGA ATTTGACTCT TTTACAAAAA 
ATTGAAGTAT TCACGTACGC TTTAGATAAT ACAAAAGGCC AAGACCTTTA TAAAATATTA 
TGGTTAAAGA GTAGGTCGTC AGAGACATGG CTAGAACGTA GAACAACTTA TACGAGATCT 
TTAGCAGTTA TGTCCATGAC TGGTTATATT CTGGGACTAG GTGATCGCCA TCCAAGCAAC 
CTGATGCTAG ATAGAATCAC CGGTAAAGTT ATCCACATTG ATTTCGGCGA TTGTTTTGAA 
GCTGCCATCT TAAGAGAAAA GTATCCAGAA AAAGTGCCAT TTAGACTAAC TAGGATGTTA 
ACATACGCAA TGGAAGTTAG TGGAATTGAA GGCAGTTTCC GAATTACTTG TGAAAATGTC 
ATGAGAGTCT TAAGAGATAA TAAAGAATCA TTAATGGCGA TCTTGGAAGC TTTTGCGCTT 
GATCCTTTGA TCCATTGGGG ATTTGATTTA CCGCCACAAA AACTTACTGA GCAAACTGGA 
ATTCCTTTGC CGTTGATTAA TCCTAGTGAA TTATTAAGGA AGGGGGCAAT TACTGTCGAA 
GAAGCGGCAA ATATGGAAGC AGAACAACAA AATGAGACCA AAAACGCCAG AGCAATGCTT 
GTTTTGAGAC GTATTACAGA TAAATTAACG GGCAATGATA TCAAGAGGTT CAATGAATTA 
GACGTCCCTG AGCAGGTTGA TAAACTGATC CAACAAGCCA CTTCTATTGA AAGGTTATGT 
CAACATTATA TTGGATGGTG CCCATTCTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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TOR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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