MAD3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MAD3
Entrez Gene ID	853439
Full Name	Mad3p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181447.3	NP_012521.3	Mad3p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MAD3	NP_012521.3
Schizosaccharomyces pombe (fission yeast)	mad3	NP_588043.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results are consistent with a role of Mad3 as the main interactor of Mad2 via the dimerization surface. Mad3 being required to stabilize the Cdc20:C-Mad2 complex implies that the complex by itself is unstable.
Title: Role of the Mad2 dimerization interface in the spindle assembly checkpoint independent of kinetochores.

Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation.
Title: Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.

Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
Title: Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.

data suggest important roles of ubiquitination in mediating IGF-1R signaling and degradation
Title: Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint.

model aimed at unifying previous analyses of checkpoint function by focusing on the Mad3-Cdc20 interaction
Title: Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint.

The following MAD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02691	NM_001181447.3 Latest version!	Saccharomyces cerevisiae S288C Mad3p (MAD3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAD3 ( NM_001181447.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012521.3 Saccharomyces cerevisiae S288C Mad3p (MAD3), partial mRNA.

Price & Availability↑

CloneID	OSi02691
Clone ID Related Accession (Same CDS sequence)	NM_001181447.3
Accession Version	NM_001181447.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mad3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181447.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAGCGT ACGCAAAGAA ACGTATCTCT TACATGCCAT CGTCACCATC ACAAAATGTT 
ATTAACTTTG AAGAAATCGA AACTCAAAAA GAAAACATTC TTCCCCTGAA GGAAGGCAGA 
TCAGCAGCGG CACTCTCGAA AGCGATACAT CAACCACTGG TGGAAATAAA CCAAGTTAAA 
TCAAGCTTCG AACAAAGACT GATAGATGAG CTCCCTGCAT TAAGCGACCC AATTACTTTG 
TATCTTGAAT ACATAAAGTG GCTGAACAAC GCCTATCCAC AAGGCGGTAA TTCCAAACAA 
TCTGGAATGC TCACCCTTTT GGAGCGATGT TTATCTCACT TAAAGGATTT GGAGAGATAT 
CGCAATGATG TTAGGTTTCT TAAAATATGG TTTTGGTACA TAGAACTTTT TACCAGAAAT 
TCATTCATGG AAAGCAGAGA CATTTTCATG TATATGTTAA GAAATGGAAT TGGTTCCGAG 
TTAGCGTCAT TTTATGAAGA ATTTACGAAT CTATTAATTC AGAAAGAAAA ATTTCAATAT 
GCCGTGAAAA TTCTACAGCT AGGAATCAAA AATAAGGCTA GGCCTAATAA GGTTTTAGAA 
GACCGGTTAA ACCATCTCCT GAGAGAACTG GGGGAGAACA ATATTCAGCT AGGAAATGAA 
ATATCGATGG ACTCATTAGA GTCTACAGTA CTTGGGAAAA CCAGATCTGA ATTTGTCAAT 
AGACTAGAGC TTGCAAATCA AAATGGCACA TCAAGTGACG TCAATTTAAC AAAAAACAAC 
GTATTTGTAG ATGGAGAAGA GTCTGATGTA GAACTATTCG AAACTCCGAA TAGAGGTGTT 
TATAGAGATG GCTGGGAAAA TTTTGACTTA AAGGCAGAAA GGAACAAAGA AAACAACCTT 
CGAATATCAT TGCTAGAAGC AAATACCAAT TTGGGTGAGC TTAAACAACA TGAGATGTTA 
TCGCAAAAAA AGAGACCATA TGATGAAAAA CTACCGATCT TTAGAGATTC TATAGGTAGA 
AGTGACCCTG TTTATCAAAT GATTAATACA AAGGACCAGA AACCAGAAAA AATTGACTGT 
AATTTTAAAC TGATTTATTG TGAAGATGAG GAATCTAAAG GTGGTAGACT AGAATTTTCG 
CTAGAAGAAG TCTTGGCAAT TTCTAGAAAC GTTTATAAAA GGGTTAGGAC AAATAGGAAA 
CATCCAAGAG AAGCAAATTT AGGGCAAGAA GAAAGCGCAA ATCAAAAAGA AGCTGAAGCA 
CAATCGAAAA GACCAAAAAT AAGTAGAAAA GCCTTAGTCT CCAAGTCGTT GACCCCCTCA 
AACCAGGGAA GAATGTTTTC TGGCGAAGAG TACATAAACT GCCCTATGAC GCCAAAGGGA 
CGAAGCACTG AGACGTCTGA TATCATCTCA GCTGTCAAGC CGCGGCAGTT AACCCCAATT 
TTAGAAATGA GAGAATCAAA TTCTTTTTCT CAAAGCAAAA ATTCTGAGAT CATTTCAGAT 
GATGACAAGT CGAGTTCGTC TTTCATATCG TACCCACCAC AGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012521.3
CDS	1..1548
Translation	MKAYAKKRISYMPSSPSQNVINFEEIETQKENILPLKEGRSAAALSKAIHQPLVEINQVKSSFEQRLIDELPALSDPITLYLEYIKWLNNAYPQGGNSKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIELFTRNSFMESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLGIKNKARPNKVLEDRLNHLLRELGENNIQLGNEISMDSLESTVLGKTRSEFVNRLELANQNGTSSDVNLTKNNVFVDGEESDVELFETPNRGVYRDGWENFDLKAERNKENNLRISLLEANTNLGELKQHEMLSQKKRPYDEKLPIFRDSIGRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEESKGGRLEFSLEEVLAISRNVYKRVRTNRKHPREANLGQEESANQKEAEAQSKRPKISRKALVSKSLTPSNQGRMFSGEEYINCPMTPKGRSTETSDIISAVKPRQLTPILEMRESNSFSQSKNSEIISDDDKSSSSFISYPPQR

Target ORF information:

RefSeq Version	NM_001181447.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mad3p (MAD3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181447.3

ORF Insert Sequence:

ATGAAAGCGT ACGCAAAGAA ACGTATCTCT TACATGCCAT CGTCACCATC ACAAAATGTT 
ATTAACTTTG AAGAAATCGA AACTCAAAAA GAAAACATTC TTCCCCTGAA GGAAGGCAGA 
TCAGCAGCGG CACTCTCGAA AGCGATACAT CAACCACTGG TGGAAATAAA CCAAGTTAAA 
TCAAGCTTCG AACAAAGACT GATAGATGAG CTCCCTGCAT TAAGCGACCC AATTACTTTG 
TATCTTGAAT ACATAAAGTG GCTGAACAAC GCCTATCCAC AAGGCGGTAA TTCCAAACAA 
TCTGGAATGC TCACCCTTTT GGAGCGATGT TTATCTCACT TAAAGGATTT GGAGAGATAT 
CGCAATGATG TTAGGTTTCT TAAAATATGG TTTTGGTACA TAGAACTTTT TACCAGAAAT 
TCATTCATGG AAAGCAGAGA CATTTTCATG TATATGTTAA GAAATGGAAT TGGTTCCGAG 
TTAGCGTCAT TTTATGAAGA ATTTACGAAT CTATTAATTC AGAAAGAAAA ATTTCAATAT 
GCCGTGAAAA TTCTACAGCT AGGAATCAAA AATAAGGCTA GGCCTAATAA GGTTTTAGAA 
GACCGGTTAA ACCATCTCCT GAGAGAACTG GGGGAGAACA ATATTCAGCT AGGAAATGAA 
ATATCGATGG ACTCATTAGA GTCTACAGTA CTTGGGAAAA CCAGATCTGA ATTTGTCAAT 
AGACTAGAGC TTGCAAATCA AAATGGCACA TCAAGTGACG TCAATTTAAC AAAAAACAAC 
GTATTTGTAG ATGGAGAAGA GTCTGATGTA GAACTATTCG AAACTCCGAA TAGAGGTGTT 
TATAGAGATG GCTGGGAAAA TTTTGACTTA AAGGCAGAAA GGAACAAAGA AAACAACCTT 
CGAATATCAT TGCTAGAAGC AAATACCAAT TTGGGTGAGC TTAAACAACA TGAGATGTTA 
TCGCAAAAAA AGAGACCATA TGATGAAAAA CTACCGATCT TTAGAGATTC TATAGGTAGA 
AGTGACCCTG TTTATCAAAT GATTAATACA AAGGACCAGA AACCAGAAAA AATTGACTGT 
AATTTTAAAC TGATTTATTG TGAAGATGAG GAATCTAAAG GTGGTAGACT AGAATTTTCG 
CTAGAAGAAG TCTTGGCAAT TTCTAGAAAC GTTTATAAAA GGGTTAGGAC AAATAGGAAA 
CATCCAAGAG AAGCAAATTT AGGGCAAGAA GAAAGCGCAA ATCAAAAAGA AGCTGAAGCA 
CAATCGAAAA GACCAAAAAT AAGTAGAAAA GCCTTAGTCT CCAAGTCGTT GACCCCCTCA 
AACCAGGGAA GAATGTTTTC TGGCGAAGAG TACATAAACT GCCCTATGAC GCCAAAGGGA 
CGAAGCACTG AGACGTCTGA TATCATCTCA GCTGTCAAGC CGCGGCAGTT AACCCCAATT 
TTAGAAATGA GAGAATCAAA TTCTTTTTCT CAAAGCAAAA ATTCTGAGAT CATTTCAGAT 
GATGACAAGT CGAGTTCGTC TTTCATATCG TACCCACCAC AGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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MAD3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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