Gene Symbol | MAD3 |
Entrez Gene ID | 853439 |
Full Name | Mad3p |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » MAD3 cDNA ORF clone
Gene Symbol | MAD3 |
Entrez Gene ID | 853439 |
Full Name | Mad3p |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001181447.3 | NP_012521.3 | Mad3p |
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
our results are consistent with a role of Mad3 as the main interactor of Mad2 via the dimerization surface. Mad3 being required to stabilize the Cdc20:C-Mad2 complex implies that the complex by itself is unstable.
Title: Role of the Mad2 dimerization interface in the spindle assembly checkpoint independent of kinetochores.
Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation.
Title: Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.
Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
Title: Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
data suggest important roles of ubiquitination in mediating IGF-1R signaling and degradation
Title: Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint.
model aimed at unifying previous analyses of checkpoint function by focusing on the Mad3-Cdc20 interaction
Title: Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint.
The following MAD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi02691 | |
Clone ID Related Accession (Same CDS sequence) | NM_001181447.3 | |
Accession Version | NM_001181447.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | Mad3p | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181447.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGAAAGCGT ACGCAAAGAA ACGTATCTCT TACATGCCAT CGTCACCATC ACAAAATGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_012521.3 |
CDS | 1..1548 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001181447.3 |
1 | ATGAAAGCGT ACGCAAAGAA ACGTATCTCT TACATGCCAT CGTCACCATC ACAAAATGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Life with 6000 genes. |
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X. |