Gene Symbol | MTR4 |
Entrez Gene ID | 853397 |
Full Name | ATP-dependent RNA helicase MTR4 |
Synonyms | DOB1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » MTR4 cDNA ORF clone
Gene Symbol | MTR4 |
Entrez Gene ID | 853397 |
Full Name | ATP-dependent RNA helicase MTR4 |
Synonyms | DOB1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001181483.1 | NP_012485.1 | ATP-dependent RNA helicase MTR4 |
Canis lupus familiaris (dog) | SKIV2L2 | XP_849676.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP009600 | XP_318627.4 |
Homo sapiens (human) | SKIV2L2 | NP_056175.3 |
Rattus norvegicus (Norway rat) | Skiv2l2 | NP_001029265.1 |
Caenorhabditis elegans (roundworm) | mtr-4 | NP_501757.1 |
Kluyveromyces lactis | KLLA0F01364g | XP_455141.1 |
Neurospora crassa | NCU03363 | XP_956298.1 |
Pan troglodytes (chimpanzee) | SKIV2L2 | XP_001145794.1 |
Schizosaccharomyces pombe (fission yeast) | mtr4 | NP_593302.1 |
Arabidopsis thaliana (thale cress) | AT1G59760 | NP_176185.1 |
Xenopus tropicalis (tropical clawed frog) | skiv2l2 | XP_002941157.1 |
Saccharomyces cerevisiae (baker's yeast) | MTR4 | NP_012485.1 |
LOC706419 | XP_002808474.1 | |
Mus musculus (house mouse) | Skiv2l2 | NP_082427.1 |
Gallus gallus (chicken) | SKIV2L2 | NP_001012962.1 |
Danio rerio (zebrafish) | skiv2l2 | NP_999933.1 |
Drosophila melanogaster (fruit fly) | l(2)35Df | NP_524929.1 |
Eremothecium gossypii | AGOS_AEL181C | NP_984680.2 |
Magnaporthe oryzae (rice blast fungus) | MGG_03931 | XP_003719933.1 |
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
report the 3.2 A resolution crystal structure of S. cerevisiae Mtr4 bound to the interacting region of Nop53, revealing how the KOW domain of the helicase recognizes the AIM sequence of Nop53 with a network of hydrophobic and electrostatic interactions
Title: Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the preribosomal protein Nop53.
Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, supporting the predicted widening of the lumen in the Rrp6-associated.Many exosome substrates exhibited clear preferences for a specific path to Rrp44. Both threading and direct access routes involve the RNA helicase Mtr4
Title: Transcriptome-wide analysis of alternative routes for RNA substrates into the exosome complex.
Mtr4p structural domain mutants in the helicase core reduced Mtr4p RNA binding, ATPase activity and unwinding activity in vitro.
Title: Mutations in Mtr4 Structural Domains Reveal Their Important Role in Regulating tRNAiMet Turnover in Saccharomyces cerevisiae and Mtr4p Enzymatic Activities In Vitro.
Study reports the identification of adaptor proteins that recruit the exosome-associated helicase, Mtr4, to unique RNA substrates. Nop53, the yeast homolog of the tumor suppressor PICT1, targets Mtr4 to pre-ribosomal particles for exosome-mediated processing, while a second adaptor Utp18 recruits Mtr4 to cleaved rRNA fragments destined for degradation by the exosome.
Title: The Exosome Is Recruited to RNA Substrates through Specific Adaptor Proteins.
The studt demonstrates that the ratchet helix modulates helicase activity and suggest that the arch domain plays a previously unrecognized role in unwinding substrates.
Title: The Mtr4 ratchet helix and arch domain both function to promote RNA unwinding.
The following MTR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi02661 | |
Clone ID Related Accession (Same CDS sequence) | NM_001181483.1 | |
Accession Version | NM_001181483.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | ATP-dependent RNA helicase MTR4 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGGATTCTA CTGATCTGTT CGATGTTTTC GAGGAAACAC CTGTTGAGCT TCCTACAGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_012485.1 |
CDS | 1..3222 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001181483.1 |
1 | ATGGATTCTA CTGATCTGTT CGATGTTTTC GAGGAAACAC CTGTTGAGCT TCCTACAGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Life with 6000 genes. |
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X. |