BCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BCK1
Entrez Gene ID	853350
Full Name	mitogen-activated protein kinase kinase kinase BCK1
Synonyms	LAS3,SAP3,SLK1,SSP31
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181528.1	NP_012440.1	mitogen-activated protein kinase kinase kinase BCK1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	BCK1	NP_012440.1
Kluyveromyces lactis	KLLA0F14190g	XP_455717.1
Eremothecium gossypii	AGOS_AFR092W	NP_985639.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data discovered that six non-essential protein kinase (PKase) disruptions (Deltabck1, Deltamkk1, Deltaslt2/Deltampk1, Deltamck1, Deltassk2 and Deltayak1) suppressed the Cas-phenotype of the Deltaptp2 Deltamsg5 double disruptant.
Title: Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant.

The following BCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02618	NM_001181528.1 Latest version!	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase BCK1 (BCK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCK1 ( NM_001181528.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012440.1 Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase BCK1 (BCK1), partial mRNA.

Price & Availability↑

CloneID	OSi02618
Clone ID Related Accession (Same CDS sequence)	NM_001181528.1
Accession Version	NM_001181528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mitogen-activated protein kinase kinase kinase BCK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCCTTTT TGAGGAAAAT AGCGGGGACA GCACATACAC ATTCTAGGTC TGATTCGAAC 
TCATCTGTGA AATTCGGCCA TCAGCCGACT AGTTCGGTAG CATCAACCAA AAGTTCAAGC 
AAAAGCCCTC GTGCAACATC TCGCAAAAGC ATTTATGATG ATATTAGAAG CCAATTTCCC 
AACCTAACCC CCAACTCTAC CTCTTCTCAG TTTTACGAAA GCACGCCAGT TATCGAACAA 
TCCTTTAATT GGACGACAGA TGACCACATC TCAGCTGGAA CGCTTGAAAA CCCAACGAGC 
TTTACAAACA GTTCTTATAA AAATGACAAT GGACCTAGTA GCCTCTCTGA TTCGAGGAAA 
TCCTCCGGTG GCAATAGCGT AAATAGTTTG TCCTTTGACA AGCTAATTCT ATCGTGGGAT 
CCTACAGACC CTGATGAATG GACAATGCAT CGCGTCACCT CATGGTTTAA ATTTCATGAT 
TTTCCAGAAT CCTGGATATT GTTTTTCAAA AAGCATCAAT TGTTTGGTCA CAGATTTATA 
AAGTTGCTTG CATATGATAA TTTCGCTGTT TATGAAAAGT ATTTGCCGCA GACTAAAACT 
GCTTCATATA CCAGGTTTCA GCAGTTATTG AAAAAAACAA TGACCAAGAA CGTAACAAAT 
AGCCATATTC GTCAGAAGAG CGCTAGCAAA CTTAAAAGTT CCAGGTCTTC CAGCGAATCG 
ATCAAATCAA AATTAAAAAA TAGTAAATCG CAAGAGGATA TTTCAAATTC TAGATCAACG 
TCAGAATCTG CATTGAGCCC AACAAAATCG GGCCCTTCCA AGACCGATGA AAAGAATTTT 
TTACATTCTA CTTCAACACA CCAAAAAACC AAAAGCGCAA GTTCACTATA CAGAAGAAGT 
TTTATATCCC TAAGAGGCTC ATCATCGAGC AATGCTTCCT CAGCAAAATC ACCTTCAAAC 
ATCAAGTTAA GTATACCGGC TCGGCCGCAC TCAATTATTG AATCTAACAG TACACTTACC 
AAATCGGCGA GCCCACCTGC ATCTCCTTCG TATCCTAGCA TATTTAGAAG ACATCACAAA 
AGTAGTTCAT CTGAGTCGTC ATTATTAAAT TCCCTTTTTG GTAGTGGAAT AGGCGAGGAA 
GCTCCAACAA AGCCTAATCC ACAAGGTCAT AGTCTGTCTA GTGAAAATTT AGCTAAAGGA 
AAATCTAAAC ACTATGAAAC TAATGTGTCT TCACCTTTAA AACAATCTTC ACTACCCACT 
TCGGATGATA AAGGTAATTT ATGGAATAAA TTCAAAAGAA AGAGCCAAAT AGGGGTTCCT 
AGCCCAAATA CGGTAGCTTA TGTAACGTCT CAAGAAACTC CATCCTTAAA ATCGAATTCG 
AGTACTGCTA CCTTAACCGT ACAAACGGCA GATGTAAATA TACCATCTCC ATCTTCATCA 
CCACCGCCAA TACCCAAAAC TGCAAACAGA AGTTTGGAGG TCATCAGCAC AGAAGATACA 
CCTAAAATTT CTTCAACCAC GGCGTCTTTT AAAGAAACGT ATCCTGATTG TATTAATCCA 
GACAAGACAG TTCCAGTGCC GGTAAATAAT CAAAAGTATA GTGTAAAGAA CTTTTTACTG 
GACCAAAAAT TTTATCCTCT GAAGAAAACA GGGTTAAATG ATAGTGAGAA TAAATATATT 
CTGGTTACCA AAGATAATGT TAGTTTTGTT CCGCTAAACT TAAAAAGTGT AGCAAAATTA 
TCCAGTTTCA AAGAATCTGC TCTCACAAAA TTGGGAATCA ATCACAAAAA TGTCACTTTC 
CATATGACAG ACTTTGATTG CGATATTGGT GCTGCAATTC CAGATGATAC TTTGGAATTT 
TTGAAAAAAA GCTTGTTTTT GAACACTTCT GGAAAAATTT ATATCAAAGA CCAAATGAAG 
CTTCAACAAA AACCGAAACC TGCTCCTCTC ACCTCAGAAA ACAATGTTCC TTTAAAATCG 
GTGAAAAGTA AGAGTTCAAT GAGGTCCGGA ACAAGCAGTC TGATAGCATC GACAGATGAT 
GTTTCCATTG TCACTTCGTC TTCTGACATA ACATCATTTG ATGAACATGC ATCAGGAAGT 
GGGCGCAGGT ACCCCCAAAC CCCGAGTTAT TACTATGACA GAGTTTCCAA TACTAATCCA 
ACTGAAGAAT TGAATTATTG GAATATTAAA GAAGTTCTTT CTCATGAGGA AAATGCACCA 
AAAATGGTTT TTAAAACAAG TCCAAAATTA GAACTCAACC TACCAGATAA AGGAAGTAAA 
TTAAATATTC CTACCCCCAT AACAGAAAAT GAAAGCAAGA GTAGTTTTCA AGTGCTAAGA 
AAAGATGAGG GGACTGAAAT TGATTTCAAT CATCGTAGGG AATCGCCTTA TACAAAACCA 
GAACTGGCAC CAAAAAGAGA AGCTCCCAAG CCTCCCGCAA ATACTTCTCC TCAGAGGACC 
TTATCAACTT CTAAACAGAA TAAACCGATC CGCCTAGTGA GGGCAAGTAC AAAAATTTCG 
AGAAGCAAAA GATCGAAACC ATTGCCGCCA CAATTATTAT CATCTCCTAT AGAAGCTAGC 
AGCTCGTCTC CTGATTCGCT TACTTCCTCA TATACTCCTG CTTCGACTCA TGTTTTGATA 
CCGCAACCTT ATAAGGGTGC AAACGATGTT ATGCGTAGGT TGAAAACAGA CCAGGACTCG 
ACGAGTACTT CCCCATCTTT GAAAATGAAA CAGAAAGTGA ATCGCTCAAA TTCAACTGTA 
TCGACTTCAA ATTCAATTTT CTATTCTCCT TCACCATTGT TAAAAAGAGG TAACTCAAAA 
AGAGTTGTTT CGTCGACATC TGCGGCCGAT ATATTTGAAG AGAATGACAT AACATTCGCG 
GATGCTCCGC CGATGTTTGA CAGCGATGAT AGTGATGACG ATTCTAGTTC ATCCGATGAC 
ATTATCTGGT CCAAGAAAAA AACAGCTCCT GAGACTAATA ATGAAAACAA AAAGGATGAG 
AAAAGCGATA ACAGTTCTAC GCATTCTGAC GAAATATTCT ATGATTCTCA AACGCAGGAC 
AAAATGGAGA GAAAGATGAC CTTTAGACCA TCTCCGGAGG TCGTTTATCA AAATTTAGAG 
AAATTCTTCC CAAGGGCTAA CTTAGATAAG CCAATCACTG AAGGAATAGC TTCACCAACA 
TCTCCGAAAT CCTTAGACAG CCTACTTTCA CCAAAGAATG TGGCTTCATC GAGAACTGAG 
CCAAGCACTC CTTCCCGTCC CGTCCCTCCT GATAGCTCAT ACGAGTTCAT ACAGGATGGA 
CTTAACGGTA AAAATAAACC ATTGAATCAA GCTAAGACAC CTAAAAGAAC AAAAACCATA 
AGAACCATTG CACATGAAGC TAGTTTAGCA AGAAAAAACT CTGTAAAACT AAAAAGACAG 
AACACCAAAA TGTGGGGTAC AAGAATGGTC GAAGTGACCG AAAACCATAT GGTGTCAATT 
AATAAAGCCA AAAATTCGAA AGGTGAGTAT AAGGAATTCG CCTGGATGAA GGGTGAAATG 
ATAGGGAAGG GATCTTTCGG TGCTGTTTAT TTATGTTTAA ACGTTACTAC AGGTGAGATG 
ATGGCCGTTA AGCAGGTTGA GGTCCCCAAG TATAGCTCAC AAAATGAAGC CATTCTAAGT 
ACCGTGGAAG CATTAAGATC TGAAGTGTCC ACGTTAAAAG ATTTAGATCA TCTTAATATT 
GTTCAATACT TAGGTTTTGA GAATAAAAAC AATATTTACA GTTTGTTTTT AGAATATGTT 
GCTGGTGGCT CCGTGGGATC CTTGATTAGA ATGTATGGAA GATTCGATGA ACCGTTGATC 
AAACATTTAA CAACACAAGT ATTAAAAGGA TTGGCATACC TACACTCGAA AGGTATTCTC 
CACAGGGATA TGAAGGCAGA CAACTTACTT TTGGATCAAG ATGGTATCTG CAAAATCAGT 
GACTTCGGAA TTTCAAGAAA ATCAAAGGAC ATATACTCTA ATTCGGATAT GACCATGCGA 
GGAACAGTCT TCTGGATGGC TCCTGAAATG GTTGATACAA AGCAAGGCTA CAGTGCAAAA 
GTTGATATAT GGTCTCTGGG ATGCATCGTT CTGGAAATGT TTGCTGGTAA GCGCCCGTGG 
TCCAACTTAG AAGTCGTCGC AGCCATGTTC AAAATTGGAA AGTCAAAATC GGCACCACCA 
ATTCCTGAGG ACACTTTACC ATTGATATCG CAAATCGGAC GAAATTTTCT GGACGCATGC 
TTCGAGATAA ATCCAGAGAA AAGGCCAACC GCTAACGAGC TTCTTTCTCA TCCTTTTAGT 
GAAGTAAATG AAACATTCAA TTTCAAATCT ACCAGACTCG CGAAGTTTAT AAAGTCAAAT 
GATAAGTTAA ACTCTTCAAA ATTAAGGATA ACCTCTCAGG AGAATAAAAC TGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012440.1
CDS	1..4437
Translation	MPFLRKIAGTAHTHSRSDSNSSVKFGHQPTSSVASTKSSSKSPRATSRKSIYDDIRSQFPNLTPNSTSSQFYESTPVIEQSFNWTTDDHISAGTLENPTSFTNSSYKNDNGPSSLSDSRKSSGGNSVNSLSFDKLILSWDPTDPDEWTMHRVTSWFKFHDFPESWILFFKKHQLFGHRFIKLLAYDNFAVYEKYLPQTKTASYTRFQQLLKKTMTKNVTNSHIRQKSASKLKSSRSSSESIKSKLKNSKSQEDISNSRSTSESALSPTKSGPSKTDEKNFLHSTSTHQKTKSASSLYRRSFISLRGSSSSNASSAKSPSNIKLSIPARPHSIIESNSTLTKSASPPASPSYPSIFRRHHKSSSSESSLLNSLFGSGIGEEAPTKPNPQGHSLSSENLAKGKSKHYETNVSSPLKQSSLPTSDDKGNLWNKFKRKSQIGVPSPNTVAYVTSQETPSLKSNSSTATLTVQTADVNIPSPSSSPPPIPKTANRSLEVISTEDTPKISSTTASFKETYPDCINPDKTVPVPVNNQKYSVKNFLLDQKFYPLKKTGLNDSENKYILVTKDNVSFVPLNLKSVAKLSSFKESALTKLGINHKNVTFHMTDFDCDIGAAIPDDTLEFLKKSLFLNTSGKIYIKDQMKLQQKPKPAPLTSENNVPLKSVKSKSSMRSGTSSLIASTDDVSIVTSSSDITSFDEHASGSGRRYPQTPSYYYDRVSNTNPTEELNYWNIKEVLSHEENAPKMVFKTSPKLELNLPDKGSKLNIPTPITENESKSSFQVLRKDEGTEIDFNHRRESPYTKPELAPKREAPKPPANTSPQRTLSTSKQNKPIRLVRASTKISRSKRSKPLPPQLLSSPIEASSSSPDSLTSSYTPASTHVLIPQPYKGANDVMRRLKTDQDSTSTSPSLKMKQKVNRSNSTVSTSNSIFYSPSPLLKRGNSKRVVSSTSAADIFEENDITFADAPPMFDSDDSDDDSSSSDDIIWSKKKTAPETNNENKKDEKSDNSSTHSDEIFYDSQTQDKMERKMTFRPSPEVVYQNLEKFFPRANLDKPITEGIASPTSPKSLDSLLSPKNVASSRTEPSTPSRPVPPDSSYEFIQDGLNGKNKPLNQAKTPKRTKTIRTIAHEASLARKNSVKLKRQNTKMWGTRMVEVTENHMVSINKAKNSKGEYKEFAWMKGEMIGKGSFGAVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDLDHLNIVQYLGFENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHSKGILHRDMKADNLLLDQDGICKISDFGISRKSKDIYSNSDMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPFSEVNETFNFKSTRLAKFIKSNDKLNSSKLRITSQENKTE

Target ORF information:

RefSeq Version	NM_001181528.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase BCK1 (BCK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181528.1

ORF Insert Sequence:

ATGCCCTTTT TGAGGAAAAT AGCGGGGACA GCACATACAC ATTCTAGGTC TGATTCGAAC 
TCATCTGTGA AATTCGGCCA TCAGCCGACT AGTTCGGTAG CATCAACCAA AAGTTCAAGC 
AAAAGCCCTC GTGCAACATC TCGCAAAAGC ATTTATGATG ATATTAGAAG CCAATTTCCC 
AACCTAACCC CCAACTCTAC CTCTTCTCAG TTTTACGAAA GCACGCCAGT TATCGAACAA 
TCCTTTAATT GGACGACAGA TGACCACATC TCAGCTGGAA CGCTTGAAAA CCCAACGAGC 
TTTACAAACA GTTCTTATAA AAATGACAAT GGACCTAGTA GCCTCTCTGA TTCGAGGAAA 
TCCTCCGGTG GCAATAGCGT AAATAGTTTG TCCTTTGACA AGCTAATTCT ATCGTGGGAT 
CCTACAGACC CTGATGAATG GACAATGCAT CGCGTCACCT CATGGTTTAA ATTTCATGAT 
TTTCCAGAAT CCTGGATATT GTTTTTCAAA AAGCATCAAT TGTTTGGTCA CAGATTTATA 
AAGTTGCTTG CATATGATAA TTTCGCTGTT TATGAAAAGT ATTTGCCGCA GACTAAAACT 
GCTTCATATA CCAGGTTTCA GCAGTTATTG AAAAAAACAA TGACCAAGAA CGTAACAAAT 
AGCCATATTC GTCAGAAGAG CGCTAGCAAA CTTAAAAGTT CCAGGTCTTC CAGCGAATCG 
ATCAAATCAA AATTAAAAAA TAGTAAATCG CAAGAGGATA TTTCAAATTC TAGATCAACG 
TCAGAATCTG CATTGAGCCC AACAAAATCG GGCCCTTCCA AGACCGATGA AAAGAATTTT 
TTACATTCTA CTTCAACACA CCAAAAAACC AAAAGCGCAA GTTCACTATA CAGAAGAAGT 
TTTATATCCC TAAGAGGCTC ATCATCGAGC AATGCTTCCT CAGCAAAATC ACCTTCAAAC 
ATCAAGTTAA GTATACCGGC TCGGCCGCAC TCAATTATTG AATCTAACAG TACACTTACC 
AAATCGGCGA GCCCACCTGC ATCTCCTTCG TATCCTAGCA TATTTAGAAG ACATCACAAA 
AGTAGTTCAT CTGAGTCGTC ATTATTAAAT TCCCTTTTTG GTAGTGGAAT AGGCGAGGAA 
GCTCCAACAA AGCCTAATCC ACAAGGTCAT AGTCTGTCTA GTGAAAATTT AGCTAAAGGA 
AAATCTAAAC ACTATGAAAC TAATGTGTCT TCACCTTTAA AACAATCTTC ACTACCCACT 
TCGGATGATA AAGGTAATTT ATGGAATAAA TTCAAAAGAA AGAGCCAAAT AGGGGTTCCT 
AGCCCAAATA CGGTAGCTTA TGTAACGTCT CAAGAAACTC CATCCTTAAA ATCGAATTCG 
AGTACTGCTA CCTTAACCGT ACAAACGGCA GATGTAAATA TACCATCTCC ATCTTCATCA 
CCACCGCCAA TACCCAAAAC TGCAAACAGA AGTTTGGAGG TCATCAGCAC AGAAGATACA 
CCTAAAATTT CTTCAACCAC GGCGTCTTTT AAAGAAACGT ATCCTGATTG TATTAATCCA 
GACAAGACAG TTCCAGTGCC GGTAAATAAT CAAAAGTATA GTGTAAAGAA CTTTTTACTG 
GACCAAAAAT TTTATCCTCT GAAGAAAACA GGGTTAAATG ATAGTGAGAA TAAATATATT 
CTGGTTACCA AAGATAATGT TAGTTTTGTT CCGCTAAACT TAAAAAGTGT AGCAAAATTA 
TCCAGTTTCA AAGAATCTGC TCTCACAAAA TTGGGAATCA ATCACAAAAA TGTCACTTTC 
CATATGACAG ACTTTGATTG CGATATTGGT GCTGCAATTC CAGATGATAC TTTGGAATTT 
TTGAAAAAAA GCTTGTTTTT GAACACTTCT GGAAAAATTT ATATCAAAGA CCAAATGAAG 
CTTCAACAAA AACCGAAACC TGCTCCTCTC ACCTCAGAAA ACAATGTTCC TTTAAAATCG 
GTGAAAAGTA AGAGTTCAAT GAGGTCCGGA ACAAGCAGTC TGATAGCATC GACAGATGAT 
GTTTCCATTG TCACTTCGTC TTCTGACATA ACATCATTTG ATGAACATGC ATCAGGAAGT 
GGGCGCAGGT ACCCCCAAAC CCCGAGTTAT TACTATGACA GAGTTTCCAA TACTAATCCA 
ACTGAAGAAT TGAATTATTG GAATATTAAA GAAGTTCTTT CTCATGAGGA AAATGCACCA 
AAAATGGTTT TTAAAACAAG TCCAAAATTA GAACTCAACC TACCAGATAA AGGAAGTAAA 
TTAAATATTC CTACCCCCAT AACAGAAAAT GAAAGCAAGA GTAGTTTTCA AGTGCTAAGA 
AAAGATGAGG GGACTGAAAT TGATTTCAAT CATCGTAGGG AATCGCCTTA TACAAAACCA 
GAACTGGCAC CAAAAAGAGA AGCTCCCAAG CCTCCCGCAA ATACTTCTCC TCAGAGGACC 
TTATCAACTT CTAAACAGAA TAAACCGATC CGCCTAGTGA GGGCAAGTAC AAAAATTTCG 
AGAAGCAAAA GATCGAAACC ATTGCCGCCA CAATTATTAT CATCTCCTAT AGAAGCTAGC 
AGCTCGTCTC CTGATTCGCT TACTTCCTCA TATACTCCTG CTTCGACTCA TGTTTTGATA 
CCGCAACCTT ATAAGGGTGC AAACGATGTT ATGCGTAGGT TGAAAACAGA CCAGGACTCG 
ACGAGTACTT CCCCATCTTT GAAAATGAAA CAGAAAGTGA ATCGCTCAAA TTCAACTGTA 
TCGACTTCAA ATTCAATTTT CTATTCTCCT TCACCATTGT TAAAAAGAGG TAACTCAAAA 
AGAGTTGTTT CGTCGACATC TGCGGCCGAT ATATTTGAAG AGAATGACAT AACATTCGCG 
GATGCTCCGC CGATGTTTGA CAGCGATGAT AGTGATGACG ATTCTAGTTC ATCCGATGAC 
ATTATCTGGT CCAAGAAAAA AACAGCTCCT GAGACTAATA ATGAAAACAA AAAGGATGAG 
AAAAGCGATA ACAGTTCTAC GCATTCTGAC GAAATATTCT ATGATTCTCA AACGCAGGAC 
AAAATGGAGA GAAAGATGAC CTTTAGACCA TCTCCGGAGG TCGTTTATCA AAATTTAGAG 
AAATTCTTCC CAAGGGCTAA CTTAGATAAG CCAATCACTG AAGGAATAGC TTCACCAACA 
TCTCCGAAAT CCTTAGACAG CCTACTTTCA CCAAAGAATG TGGCTTCATC GAGAACTGAG 
CCAAGCACTC CTTCCCGTCC CGTCCCTCCT GATAGCTCAT ACGAGTTCAT ACAGGATGGA 
CTTAACGGTA AAAATAAACC ATTGAATCAA GCTAAGACAC CTAAAAGAAC AAAAACCATA 
AGAACCATTG CACATGAAGC TAGTTTAGCA AGAAAAAACT CTGTAAAACT AAAAAGACAG 
AACACCAAAA TGTGGGGTAC AAGAATGGTC GAAGTGACCG AAAACCATAT GGTGTCAATT 
AATAAAGCCA AAAATTCGAA AGGTGAGTAT AAGGAATTCG CCTGGATGAA GGGTGAAATG 
ATAGGGAAGG GATCTTTCGG TGCTGTTTAT TTATGTTTAA ACGTTACTAC AGGTGAGATG 
ATGGCCGTTA AGCAGGTTGA GGTCCCCAAG TATAGCTCAC AAAATGAAGC CATTCTAAGT 
ACCGTGGAAG CATTAAGATC TGAAGTGTCC ACGTTAAAAG ATTTAGATCA TCTTAATATT 
GTTCAATACT TAGGTTTTGA GAATAAAAAC AATATTTACA GTTTGTTTTT AGAATATGTT 
GCTGGTGGCT CCGTGGGATC CTTGATTAGA ATGTATGGAA GATTCGATGA ACCGTTGATC 
AAACATTTAA CAACACAAGT ATTAAAAGGA TTGGCATACC TACACTCGAA AGGTATTCTC 
CACAGGGATA TGAAGGCAGA CAACTTACTT TTGGATCAAG ATGGTATCTG CAAAATCAGT 
GACTTCGGAA TTTCAAGAAA ATCAAAGGAC ATATACTCTA ATTCGGATAT GACCATGCGA 
GGAACAGTCT TCTGGATGGC TCCTGAAATG GTTGATACAA AGCAAGGCTA CAGTGCAAAA 
GTTGATATAT GGTCTCTGGG ATGCATCGTT CTGGAAATGT TTGCTGGTAA GCGCCCGTGG 
TCCAACTTAG AAGTCGTCGC AGCCATGTTC AAAATTGGAA AGTCAAAATC GGCACCACCA 
ATTCCTGAGG ACACTTTACC ATTGATATCG CAAATCGGAC GAAATTTTCT GGACGCATGC 
TTCGAGATAA ATCCAGAGAA AAGGCCAACC GCTAACGAGC TTCTTTCTCA TCCTTTTAGT 
GAAGTAAATG AAACATTCAA TTTCAAATCT ACCAGACTCG CGAAGTTTAT AAAGTCAAAT 
GATAAGTTAA ACTCTTCAAA ATTAAGGATA ACCTCTCAGG AGAATAAAAC TGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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BCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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