ORF » Species Summary » Saccharomyces cerevisiae S288C » IME2 cDNA ORF clone

IME2 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following IME2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IME2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02607 NM_001181539.1
Latest version!		 Saccharomyces cerevisiae S288C protein kinase IME2 (IME2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    IME2 ( NM_001181539.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012429.1 Saccharomyces cerevisiae S288C protein kinase IME2 (IME2), partial mRNA.
    Price & Availability↑
    CloneID OSi02607
    Clone ID Related Accession (Same CDS sequence) NM_001181539.1
    Accession Version NM_001181539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product protein kinase IME2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTTGAAA AACGTAGTAG ACAGAGTTCC AGTAGTGGCT CAGAGTTCAG TGTTCCACCT 
    GATGTCGATA ACCCGCCACT TTCTATCCCT TTGAAGACTT TATCAGACAG ATATCAGCTG 
    ATCGAAAAAC TAGGTGCAGG ATCATTTGGT TGTGTAACTT TAGCCAAAGC GCAATTTCCT 
    TTATCGAACA TATTGGGTAA ACAACATGAT ATAAGGGGTA CTTTAATGGA TCAGCCTAAA 
    AATGGTCACC AAAACTATAT AACAAAGACA CAAGGTGTAG TGGCTATAAA GACAATGATG 
    ACAAAACTGC ATACACTCCA GGATTATACG AGGGTCAGAG AAATTAAGTT CATACTGGCA 
    ATCCCAGCGA ATGACCATCT GATACAAATT TTTGAGGTCT TTATTGACTC TGAAAATTAC 
    CAATTACATA TCGTAATGGA ATGTATGGAG CAAAACCTAT ATCAAATGAT GAAACATAGA 
    AGGCGCCGAG TTTTTTCGAT TCCTTCTTTG AAATCTATTC TATCGCAGAT ACTGGCTGGG 
    TTAAAACACA TCCACGAACA TAATTTCTTC CACAGAGATT TAAAACCTGA AAATATTCTT 
    ATCACACCAA GTACCCAGTA TTTTGAAAAG GAGTACATGA ACCAGATTGG ATATCAAGAT 
    AACTATGTCA TTAAACTTGC AGATTTTGGT TTGGCACGCC ATGTTGAAAA TAAAAACCCG 
    TATACGGCCT ACGTTTCCAC AAGATGGTAT AGATCGCCTG AAATATTATT AAGAAGTGGT 
    TACTATTCCA AACCTTTGGA TATTTGGGCA TTCGGGTGCG TGGCGGTGGA AGTTACTGTA 
    TTCAGAGCTC TCTTCCCCGG TGCTAACGAA ATCGATCAGA TATGGAAAAT TTTAGAAGTT 
    CTTGGTACAC CTATCAAACG TTCTGATTTT GTTAATACAA ACCACATCAC AGCTCCACCA 
    CCTGGTGGAT TTTGGGATGA TGCCAGTAAT TTGGTCCATA AATTAAACTT GAAATTGCCT 
    TATGTAGAAG GTTCATCGTT GGATCATCTA CTTTCTAGCT CCCAACTATC CGATTTATCT 
    GAGGTTGTCA AAAAGTGTTT AAGATGGGAT CCTAATGAAA GGGCAACAGC CCAAGAGCTT 
    TGTGAAATGC CATTTTTTGA AAACACCGTG GCATCCCAAG TAGACGCAAG AGGCAATGTG 
    ACTAATACAG AACAAGCATT GATTTTTGCA GGTATAAATC CAGTAGCGAC CAACACAAAG 
    CCCATATATT TTAACTCTTC AACAAAATTG CCCGCAGAAA CAGAGTCTAA TGATATTGAT 
    ATCAGCAACA ACGATCACGA TTCTCATGCT ATGTGCTCAC CAACATTAAA TCAAGAAAAA 
    CTAACTTTGG TAGAATTTTT AAACGAATTC GTTGAAGAAG ATAATGATGA TCACTCAATA 
    CCTGATGTTG GAACAGATTC TACCATTTCA GATTCTATTG ATGAGACTGA ATTATCAAAA 
    GAAATTCGTA ATAATTTGGC CCTTTGTCAA CTACCAGATG AAGAAGTCTT AGATCATTCA 
    TTATCGAATA TTAGGCAGTT GACAAATGAT ATTGAAATTA TAAATAAAGA TGAAGCAGAC 
    AACATGGAAC AGCTATTTTT TGACCTGGAA ATTCCTGAAA AGGATGAGTT TCAGAGAAAA 
    CAACCATTTA ATGAACATGC AGATATTGAT GAAGACATCG TTCTACCATA TGTGAACAAC 
    TCAAATTACA CACATACAGA TAGGTCACAT CATAGAGGTG ATAATGTTTT GGGTGATGCC 
    TCTTTAGGCG ATTCGTTTAA TTCAATGCCA GATTTTACTC CAAGAAATTT CTTGATACCT 
    ACATTAAAAA AATCACGGGA AAAGTTCGAG CCTCATCTAT CCAATTCAAA CCAACATTTT 
    GGAAATGTAA CCTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012429.1
    CDS1..1938
    Translation

    Target ORF information:

    RefSeq Version NM_001181539.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C protein kinase IME2 (IME2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTTGAAA AACGTAGTAG ACAGAGTTCC AGTAGTGGCT CAGAGTTCAG TGTTCCACCT 
    GATGTCGATA ACCCGCCACT TTCTATCCCT TTGAAGACTT TATCAGACAG ATATCAGCTG 
    ATCGAAAAAC TAGGTGCAGG ATCATTTGGT TGTGTAACTT TAGCCAAAGC GCAATTTCCT 
    TTATCGAACA TATTGGGTAA ACAACATGAT ATAAGGGGTA CTTTAATGGA TCAGCCTAAA 
    AATGGTCACC AAAACTATAT AACAAAGACA CAAGGTGTAG TGGCTATAAA GACAATGATG 
    ACAAAACTGC ATACACTCCA GGATTATACG AGGGTCAGAG AAATTAAGTT CATACTGGCA 
    ATCCCAGCGA ATGACCATCT GATACAAATT TTTGAGGTCT TTATTGACTC TGAAAATTAC 
    CAATTACATA TCGTAATGGA ATGTATGGAG CAAAACCTAT ATCAAATGAT GAAACATAGA 
    AGGCGCCGAG TTTTTTCGAT TCCTTCTTTG AAATCTATTC TATCGCAGAT ACTGGCTGGG 
    TTAAAACACA TCCACGAACA TAATTTCTTC CACAGAGATT TAAAACCTGA AAATATTCTT 
    ATCACACCAA GTACCCAGTA TTTTGAAAAG GAGTACATGA ACCAGATTGG ATATCAAGAT 
    AACTATGTCA TTAAACTTGC AGATTTTGGT TTGGCACGCC ATGTTGAAAA TAAAAACCCG 
    TATACGGCCT ACGTTTCCAC AAGATGGTAT AGATCGCCTG AAATATTATT AAGAAGTGGT 
    TACTATTCCA AACCTTTGGA TATTTGGGCA TTCGGGTGCG TGGCGGTGGA AGTTACTGTA 
    TTCAGAGCTC TCTTCCCCGG TGCTAACGAA ATCGATCAGA TATGGAAAAT TTTAGAAGTT 
    CTTGGTACAC CTATCAAACG TTCTGATTTT GTTAATACAA ACCACATCAC AGCTCCACCA 
    CCTGGTGGAT TTTGGGATGA TGCCAGTAAT TTGGTCCATA AATTAAACTT GAAATTGCCT 
    TATGTAGAAG GTTCATCGTT GGATCATCTA CTTTCTAGCT CCCAACTATC CGATTTATCT 
    GAGGTTGTCA AAAAGTGTTT AAGATGGGAT CCTAATGAAA GGGCAACAGC CCAAGAGCTT 
    TGTGAAATGC CATTTTTTGA AAACACCGTG GCATCCCAAG TAGACGCAAG AGGCAATGTG 
    ACTAATACAG AACAAGCATT GATTTTTGCA GGTATAAATC CAGTAGCGAC CAACACAAAG 
    CCCATATATT TTAACTCTTC AACAAAATTG CCCGCAGAAA CAGAGTCTAA TGATATTGAT 
    ATCAGCAACA ACGATCACGA TTCTCATGCT ATGTGCTCAC CAACATTAAA TCAAGAAAAA 
    CTAACTTTGG TAGAATTTTT AAACGAATTC GTTGAAGAAG ATAATGATGA TCACTCAATA 
    CCTGATGTTG GAACAGATTC TACCATTTCA GATTCTATTG ATGAGACTGA ATTATCAAAA 
    GAAATTCGTA ATAATTTGGC CCTTTGTCAA CTACCAGATG AAGAAGTCTT AGATCATTCA 
    TTATCGAATA TTAGGCAGTT GACAAATGAT ATTGAAATTA TAAATAAAGA TGAAGCAGAC 
    AACATGGAAC AGCTATTTTT TGACCTGGAA ATTCCTGAAA AGGATGAGTT TCAGAGAAAA 
    CAACCATTTA ATGAACATGC AGATATTGAT GAAGACATCG TTCTACCATA TGTGAACAAC 
    TCAAATTACA CACATACAGA TAGGTCACAT CATAGAGGTG ATAATGTTTT GGGTGATGCC 
    TCTTTAGGCG ATTCGTTTAA TTCAATGCCA GATTTTACTC CAAGAAATTT CTTGATACCT 
    ACATTAAAAA AATCACGGGA AAAGTTCGAG CCTCATCTAT CCAATTCAAA CCAACATTTT 
    GGAAATGTAA CCTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
    The EMBO journal15(9)2031-49(1996 May)
    Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L


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