PBS2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PBS2
Entrez Gene ID	853313
Full Name	mitogen-activated protein kinase kinase PBS2
Synonyms	HOG4,SFS4,SSK4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181561.2	NP_012407.2	mitogen-activated protein kinase kinase PBS2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PBS2	NP_012407.2
Kluyveromyces lactis	KLLA0E15313g	XP_454640.1
Eremothecium gossypii	AGOS_AFL217C	NP_985333.1
Danio rerio (zebrafish)	map2k4b	NP_001082890.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that that two alternative MAPKKs, Pbs2 and Mkk2, can be forced to functionally replace the mating MAPKK Ste7, but only if the proper set of recruitment interactions are grafted onto their catalytic domains.
Title: Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase.

In the N-terminal noncatalytic region of Pbs2, there is a specific Hog1-binding domain, HBD-1.
Title: Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase.

hypersensitivity to salt and other stress conditions was due to the deletion of 5 base pairs close to position 936 bp in the PBS2 gene essential for HOG signal pathway regulation under salt stress
Title: [Identification of the gene correlated with salt stress in the Saccharomyces cerevisiae 263-H9 mutant].

Here we show that by replacing C-terminal region of Dpbs2p with the homologous region of Pbs2p we could abrogate partial complementation exhibited by Dpbs2p and this was achieved due to increase in nuclear translocation of Hog1p.
Title: Evidence that C-terminal non-kinase domain of Pbs2p has a role in high osmolarity-induced nuclear localization of Hog1p.

The following PBS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PBS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02587	NM_001181561.2 Latest version!	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase PBS2 (PBS2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PBS2 ( NM_001181561.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012407.2 Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase PBS2 (PBS2), partial mRNA.

Price & Availability↑

CloneID	OSi02587
Clone ID Related Accession (Same CDS sequence)	NM_001181561.2
Accession Version	NM_001181561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mitogen-activated protein kinase kinase PBS2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Apr 26, 2011 this sequence version replaced NM_001181561.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAGACA AGTTTGCTAA CCTCAGTCTC CATGAGAAAA CTGGTAAGTC ATCTATCCAA 
TTAAACGAGC AAACAGGCTC AGATAATGGC TCTGCTGTCA AGAGAACATC TTCGACGTCC 
TCGCACTACA ATAACATCAA CGCTGACCTT CATGCTCGTG TAAAAGCTTT TCAAGAACAA 
CGTGCATTGA AAAGGTCTGC CAGCGTGGGC AGTAATCAAA GCGAGCAAGA CAAAGGCAGT 
TCACAATCAC CTAAACATAT TCAGCAGATT GTTAATAAGC CATTGCCGCC TCTTCCCGTA 
GCAGGAAGTT CTAAGGTTTC ACAAAGAATG AGTAGCCAAG TCGTGCAAGC GTCCTCCAAG 
AGCACTCTTA AGAACGTTCT GGACAATCAA GAAACACAAA ACATTACCGA CGTAAATATT 
AACATCGATA CAACCAAAAT TACCGCCACA ACAATTGGTG TAAATACTGG CCTACCTGCT 
ACTGACATTA CGCCGTCAGT TTCTAATACT GCATCAGCAA CACATAAGGC GCAATTGCTG 
AATCCTAACA GAAGGGCACC AAGAAGGCCG CTTTCTACCC AGCACCCTAC AAGACCAAAT 
GTTGCCCCGC ATAAGGCCCC TGCTATAATC AACACACCAA AACAAAGTTT AAGTGCCCGT 
CGAGGGCTCA AATTACCACC AGGAGGAATG TCATTAAAAA TGCCCACTAA AACAGCTCAA 
CAGCCGCAGC AGTTTGCCCC AAGCCCTTCA AACAAAAAAC ATATAGAAAC CTTATCAAAC 
AGCAAAGTTG TTGAAGGGAA AAGATCGAAT CCGGGTTCTT TGATAAATGG TGTGCAAAGC 
ACATCCACCT CATCAAGTAC CGAAGGCCCA CATGACACTG TAGGCACTAC ACCCAGAACT 
GGAAACAGCA ACAACTCTTC AAATTCTGGT AGTAGTGGTG GTGGTGGTCT TTTCGCAAAT 
TTCTCGAAAT ACGTGGATAT CAAATCCGGC TCTTTGAATT TTGCAGGCAA ACTATCGCTA 
TCCTCTAAAG GAATAGATTT CAGCAATGGT TCTAGTTCGA GAATTACATT GGACGAACTA 
GAATTTTTGG ATGAACTGGG TCATGGTAAC TATGGTAACG TCTCAAAGGT ACTGCATAAG 
CCCACAAATG TTATTATGGC GACGAAGGAA GTCCGTTTGG AGCTAGATGA GGCTAAATTT 
AGACAAATTT TAATGGAACT AGAAGTTTTG CATAAATGCA ATTCTCCCTA TATTGTGGAT 
TTTTATGGTG CATTCTTTAT TGAGGGCGCC GTCTACATGT GTATGGAATA CATGGATGGT 
GGTTCCTTGG ATAAAATATA CGACGAATCA TCTGAAATCG GCGGCATTGA TGAACCTCAG 
CTAGCGTTTA TTGCCAATGC TGTCATTCAT GGACTAAAAG AACTCAAAGA GCAGCATAAT 
ATCATACACA GAGATGTCAA ACCAACAAAT ATTTTATGTT CAGCCAACCA AGGCACCGTA 
AAGCTGTGCG ATTTCGGTGT TTCTGGTAAT TTGGTGGCAT CTTTAGCGAA GACTAATATT 
GGTTGTCAGT CATACATGGC ACCTGAACGA ATCAAATCGT TGAATCCAGA TAGAGCCACC 
TATACCGTAC AGTCAGACAT CTGGTCTTTA GGTTTAAGCA TTCTGGAAAT GGCACTAGGT 
AGATATCCGT ATCCACCAGA AACATACGAC AACATTTTCT CTCAATTGAG CGCTATTGTT 
GATGGGCCGC CACCGAGATT ACCTTCAGAT AAATTCAGTT CTGACGCACA AGATTTTGTT 
TCTTTATGTC TACAAAAGAT TCCGGAAAGA AGACCTACAT ACGCAGCTTT AACAGAGCAT 
CCTTGGTTAG TAAAATACAG AAACCAGGAT GTCCACATGA GTGAGTATAT CACTGAACGA 
TTAGAAAGGC GCAACAAAAT CTTACGGGAA CGTGGTGAGA ATGGTTTATC TAAAAATGTA 
CCGGCATTAC ATATGGGTGG TTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012407.2
CDS	1..2007
Translation	MEDKFANLSLHEKTGKSSIQLNEQTGSDNGSAVKRTSSTSSHYNNINADLHARVKAFQEQRALKRSASVGSNQSEQDKGSSQSPKHIQQIVNKPLPPLPVAGSSKVSQRMSSQVVQASSKSTLKNVLDNQETQNITDVNINIDTTKITATTIGVNTGLPATDITPSVSNTASATHKAQLLNPNRRAPRRPLSTQHPTRPNVAPHKAPAIINTPKQSLSARRGLKLPPGGMSLKMPTKTAQQPQQFAPSPSNKKHIETLSNSKVVEGKRSNPGSLINGVQSTSTSSSTEGPHDTVGTTPRTGNSNNSSNSGSSGGGGLFANFSKYVDIKSGSLNFAGKLSLSSKGIDFSNGSSSRITLDELEFLDELGHGNYGNVSKVLHKPTNVIMATKEVRLELDEAKFRQILMELEVLHKCNSPYIVDFYGAFFIEGAVYMCMEYMDGGSLDKIYDESSEIGGIDEPQLAFIANAVIHGLKELKEQHNIIHRDVKPTNILCSANQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDRATYTVQSDIWSLGLSILEMALGRYPYPPETYDNIFSQLSAIVDGPPPRLPSDKFSSDAQDFVSLCLQKIPERRPTYAALTEHPWLVKYRNQDVHMSEYITERLERRNKILRERGENGLSKNVPALHMGGL

Target ORF information:

RefSeq Version	NM_001181561.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase PBS2 (PBS2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181561.2

ORF Insert Sequence:

ATGGAAGACA AGTTTGCTAA CCTCAGTCTC CATGAGAAAA CTGGTAAGTC ATCTATCCAA 
TTAAACGAGC AAACAGGCTC AGATAATGGC TCTGCTGTCA AGAGAACATC TTCGACGTCC 
TCGCACTACA ATAACATCAA CGCTGACCTT CATGCTCGTG TAAAAGCTTT TCAAGAACAA 
CGTGCATTGA AAAGGTCTGC CAGCGTGGGC AGTAATCAAA GCGAGCAAGA CAAAGGCAGT 
TCACAATCAC CTAAACATAT TCAGCAGATT GTTAATAAGC CATTGCCGCC TCTTCCCGTA 
GCAGGAAGTT CTAAGGTTTC ACAAAGAATG AGTAGCCAAG TCGTGCAAGC GTCCTCCAAG 
AGCACTCTTA AGAACGTTCT GGACAATCAA GAAACACAAA ACATTACCGA CGTAAATATT 
AACATCGATA CAACCAAAAT TACCGCCACA ACAATTGGTG TAAATACTGG CCTACCTGCT 
ACTGACATTA CGCCGTCAGT TTCTAATACT GCATCAGCAA CACATAAGGC GCAATTGCTG 
AATCCTAACA GAAGGGCACC AAGAAGGCCG CTTTCTACCC AGCACCCTAC AAGACCAAAT 
GTTGCCCCGC ATAAGGCCCC TGCTATAATC AACACACCAA AACAAAGTTT AAGTGCCCGT 
CGAGGGCTCA AATTACCACC AGGAGGAATG TCATTAAAAA TGCCCACTAA AACAGCTCAA 
CAGCCGCAGC AGTTTGCCCC AAGCCCTTCA AACAAAAAAC ATATAGAAAC CTTATCAAAC 
AGCAAAGTTG TTGAAGGGAA AAGATCGAAT CCGGGTTCTT TGATAAATGG TGTGCAAAGC 
ACATCCACCT CATCAAGTAC CGAAGGCCCA CATGACACTG TAGGCACTAC ACCCAGAACT 
GGAAACAGCA ACAACTCTTC AAATTCTGGT AGTAGTGGTG GTGGTGGTCT TTTCGCAAAT 
TTCTCGAAAT ACGTGGATAT CAAATCCGGC TCTTTGAATT TTGCAGGCAA ACTATCGCTA 
TCCTCTAAAG GAATAGATTT CAGCAATGGT TCTAGTTCGA GAATTACATT GGACGAACTA 
GAATTTTTGG ATGAACTGGG TCATGGTAAC TATGGTAACG TCTCAAAGGT ACTGCATAAG 
CCCACAAATG TTATTATGGC GACGAAGGAA GTCCGTTTGG AGCTAGATGA GGCTAAATTT 
AGACAAATTT TAATGGAACT AGAAGTTTTG CATAAATGCA ATTCTCCCTA TATTGTGGAT 
TTTTATGGTG CATTCTTTAT TGAGGGCGCC GTCTACATGT GTATGGAATA CATGGATGGT 
GGTTCCTTGG ATAAAATATA CGACGAATCA TCTGAAATCG GCGGCATTGA TGAACCTCAG 
CTAGCGTTTA TTGCCAATGC TGTCATTCAT GGACTAAAAG AACTCAAAGA GCAGCATAAT 
ATCATACACA GAGATGTCAA ACCAACAAAT ATTTTATGTT CAGCCAACCA AGGCACCGTA 
AAGCTGTGCG ATTTCGGTGT TTCTGGTAAT TTGGTGGCAT CTTTAGCGAA GACTAATATT 
GGTTGTCAGT CATACATGGC ACCTGAACGA ATCAAATCGT TGAATCCAGA TAGAGCCACC 
TATACCGTAC AGTCAGACAT CTGGTCTTTA GGTTTAAGCA TTCTGGAAAT GGCACTAGGT 
AGATATCCGT ATCCACCAGA AACATACGAC AACATTTTCT CTCAATTGAG CGCTATTGTT 
GATGGGCCGC CACCGAGATT ACCTTCAGAT AAATTCAGTT CTGACGCACA AGATTTTGTT 
TCTTTATGTC TACAAAAGAT TCCGGAAAGA AGACCTACAT ACGCAGCTTT AACAGAGCAT 
CCTTGGTTAG TAAAATACAG AAACCAGGAT GTCCACATGA GTGAGTATAT CACTGAACGA 
TTAGAAAGGC GCAACAAAAT CTTACGGGAA CGTGGTGAGA ATGGTTTATC TAAAAATGTA 
CCGGCATTAC ATATGGGTGG TTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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PBS2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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