FAR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FAR1
Entrez Gene ID	853283
Full Name	cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181590.1	NP_012378.1	cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This papers suggests that Far1 engages the cyclin-Cdk complex in a way that disrupts multiple distinct functions, including both substrate docking and kinase activity but perhaps also others such as Cks1-mediated processivity.
Title: Regulation of cyclin-substrate docking by a G1 arrest signaling pathway and the Cdk inhibitor Far1.

Studied FAR1 overexpression, which not only coordinately increases RNA and protein accumulation, but induces strong transcriptional remodeling.
Title: Overexpression of Far1, a cyclin-dependent kinase inhibitor, induces a large transcriptional reprogramming in which RNA synthesis senses Far1 in a Sfp1-mediated way.

Cln3 must overcome Far1 to trigger Cln-Cdc28 activation, which then turns on transcription; a second threshold is required together with the Cln3/Far1 threshold for carbon source modulation of protein content per cell at the onset of DNA replication
Title: A cell sizer network involving Cln3 and Far1 controls entrance into S phase in the mitotic cycle of budding yeast.

Gbetagamma not only targets Far1p to the correct site but may also trigger a conformational change in Far1p that is required for its ability to activate Cdc24p in vivo
Title: Site-specific regulation of the GEF Cdc24p by the scaffold protein Far1p during yeast mating.

The following FAR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02562	NM_001181590.1 Latest version!	Saccharomyces cerevisiae S288C cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1 (FAR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAR1 ( NM_001181590.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012378.1 Saccharomyces cerevisiae S288C cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1 (FAR1), partial mRNA.

Price & Availability↑

CloneID	OSi02562
Clone ID Related Accession (Same CDS sequence)	NM_001181590.1
Accession Version	NM_001181590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGACAC CAACAAGAGT TTCGTTTGAA AAAAAAATAC ACACTCCACC AAGTGGAGAT 
CGTGACGCTG AAAGATCACC ACCGAAGAAA TTTCTAAGAG GTTTATCGGG TAAAGTTTTT 
AGAAAGACAC CAGAATTTAA GAAGCAACAG ATGCCCACAT TTGGGTATAT TGAAGAGAGT 
CAATTTACTC CAAATCTTGG CCTAATGATG TCCAAAAGAG GCAATATTCC TAAACCACTG 
AATCTTTCCA AGCCTATATC TCCCCCACCA AGTTTGAAGA AAACAGCGGG ATCAGTAGCA 
TCTGGTTTTT CGAAAACAGG ACAACTTAGC GCTTTACAAT CGCCAGTAAA TATCACAAGT 
AGCAACAAAT ACAACATCAA AGCTACAAAT TTAACTACTA GTTTACTCCG AGAGTCGATT 
TCTGATTCTA CAACTATGTG TGATACTTTG TCAGACATCA ATTTAACGGT TATGGATGAA 
GATTATCGCA TTGACGGCGA TTCATATTAC GAAGAAGATT CACCAACTTT CATGATCAGC 
CTGGAAAGAA ACATAAAAAA ATGCAATTCA CAATTCAGTC CAAAAAGGTA CATAGGTGAG 
AAATGTTTAA TCTGTGAGGA AAGTATTAGT TCCACATTTA CCGGTGAAAA GGTTGTGGAG 
TCTACGTGTT CACACACTAG CCACTATAAC TGTTATTTGA TGCTATTTGA AACTCTTTAT 
TTCCAAGGCA AGTTTCCTGA GTGTAAGATA TGTGGCGAAG TGAGCAAGCC AAAGGATAAA 
GACATTGTTC CTGAAATGGT ATCCAAACTT CTAACGGGCG CAGGCGCTCA TGATGATGGT 
CCGTCATCCA ATATGCAACA GCAATGGATT GATTTGAAAA CGGCGAGAAG CTTCACAGGT 
GAATTTCCTC AGTTTACACC ACAGGAGCAG CTCATTCGTA CTGCTGATAT TTCATGTGAT 
GGGTTCAGGA CACCACGCTT ATCGAATAGC AATCAATTTG AGGCCGTTAG CTATCTTGAT 
AGCCCATTTT TAAATTCACC ATTCGTTAAT AAAATGGCAA CTACAGACCC TTTTGATTTA 
AGTGATGATG AAAAACTGGA CTGTGATGAT GAAATAGACG AAAGTGCTGC TGAAGTGTGG 
TTTAGTAAAA CGGGTGGTGA ACATGTTATG GTCAGTGTGA AGTTTCAAGA AATGCGGACC 
AGTGATGATT TAGGGGTCTT GCAAGATGTG AATCATGTGG ACCACGAAGA ACTAGAGGAA 
AGGGAAAAGG AATGGAAAAA GAAAATTGAT CAATATATCG AAACAAATGT GGATAAGGAT 
AGCGAATTTG GCAGTTTGAT CTTGTTTGAC AAACTAATGT ATTCTGATGA TGGCGAACAA 
TGGGTAGACA ATAACCTTGT AATATTATTC TCCAAATTTC TAGTTTTGTT TGATTTTGAA 
GAAATGAAGA TCCTGGGTAA GATTCCTAGA GACCAGTTTT ACCAGGTAAT CAAGTTCAAT 
GAGGACGTAC TACTTTGTAG TTTGAAGAGC ACTAATATAC CTGAAATATA CTTGAGGTTC 
AACGAAAACT GCGAAAAGTG GTTGCTTCCC AAATGGAAGT ATTGTCTAGA AAATTCTTCA 
CTGGAGACAC TACCATTAAG TGAGATTGTT TCTACAGTGA AGGAACTCTC TCATGTGAAT 
ATAATAGGCG CTTTAGGAGC GCCGCCTGAT GTTATATCAG CACAATCCCA TGATTCTCGT 
CTACCGTGGA AAAGATTGCA CAGTGATACG CCGCTAAAAC TTATTGTATG TTTGAATTTG 
TCGCATGCTG ATGGGGAACT GTATCGCAAG AGAGTTCTAA AGAGTGTTCA TCAAATATTG 
GATGGATTAA ACACAGACGA TTTACTGGGA ATAGTTGTTG TTGGAAGAGA TGGATCCGGT 
GTAGTAGGCC CATTTGGCAC CTTCATCGGA ATGATAAATA AAAATTGGGA CGGATGGACA 
ACTTTCTTGG ATAATTTAGA AGTGGTTAAT CCAAACGTCT TCCGCGATGA AAAGCAACAG 
TATAAGGTAA CATTACAAAC TTGTGAAAGA TTAGCGTCAA CAAGTGCATA CGTGGATACA 
GATGACCATA TTGCAACTGG ATACGCCAAG CAAATCTTGG TCCTCAACGG GAGTGACGTT 
GTTGACATTG AGCATGATCA GAAATTAAAA AAGGCCTTTG ACCAGTTATC TTACCATTGG 
AGATACGAAA TTTCGCAACG CCGCATGACT CCATTGAACG CTAGCATTAA GCAGTTTTTA 
GAGGAGCTGC ACACTAAGCG TTATCTAGAT GTAACTCTTC GTCTACCACA AGCTACATTC 
GAGCAAGTAT ATTTGGGGGA TATGGCTGCG GGTGAACAAA AAACGCGTCT AATTATGGAT 
GAACACCCGC ATTCTAGTTT GATAGAAATA GAATATTTTG ACCTGGTAAA GCAGCAAAGA 
ATTCATCAGA CCCTGGAAGT TCCCAACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012378.1
CDS	1..2493
Translation	MKTPTRVSFEKKIHTPPSGDRDAERSPPKKFLRGLSGKVFRKTPEFKKQQMPTFGYIEESQFTPNLGLMMSKRGNIPKPLNLSKPISPPPSLKKTAGSVASGFSKTGQLSALQSPVNITSSNKYNIKATNLTTSLLRESISDSTTMCDTLSDINLTVMDEDYRIDGDSYYEEDSPTFMISLERNIKKCNSQFSPKRYIGEKCLICEESISSTFTGEKVVESTCSHTSHYNCYLMLFETLYFQGKFPECKICGEVSKPKDKDIVPEMVSKLLTGAGAHDDGPSSNMQQQWIDLKTARSFTGEFPQFTPQEQLIRTADISCDGFRTPRLSNSNQFEAVSYLDSPFLNSPFVNKMATTDPFDLSDDEKLDCDDEIDESAAEVWFSKTGGEHVMVSVKFQEMRTSDDLGVLQDVNHVDHEELEEREKEWKKKIDQYIETNVDKDSEFGSLILFDKLMYSDDGEQWVDNNLVILFSKFLVLFDFEEMKILGKIPRDQFYQVIKFNEDVLLCSLKSTNIPEIYLRFNENCEKWLLPKWKYCLENSSLETLPLSEIVSTVKELSHVNIIGALGAPPDVISAQSHDSRLPWKRLHSDTPLKLIVCLNLSHADGELYRKRVLKSVHQILDGLNTDDLLGIVVVGRDGSGVVGPFGTFIGMINKNWDGWTTFLDNLEVVNPNVFRDEKQQYKVTLQTCERLASTSAYVDTDDHIATGYAKQILVLNGSDVVDIEHDQKLKKAFDQLSYHWRYEISQRRMTPLNASIKQFLEELHTKRYLDVTLRLPQATFEQVYLGDMAAGEQKTRLIMDEHPHSSLIEIEYFDLVKQQRIHQTLEVPNL

Target ORF information:

RefSeq Version	NM_001181590.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1 (FAR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181590.1

ORF Insert Sequence:

ATGAAGACAC CAACAAGAGT TTCGTTTGAA AAAAAAATAC ACACTCCACC AAGTGGAGAT 
CGTGACGCTG AAAGATCACC ACCGAAGAAA TTTCTAAGAG GTTTATCGGG TAAAGTTTTT 
AGAAAGACAC CAGAATTTAA GAAGCAACAG ATGCCCACAT TTGGGTATAT TGAAGAGAGT 
CAATTTACTC CAAATCTTGG CCTAATGATG TCCAAAAGAG GCAATATTCC TAAACCACTG 
AATCTTTCCA AGCCTATATC TCCCCCACCA AGTTTGAAGA AAACAGCGGG ATCAGTAGCA 
TCTGGTTTTT CGAAAACAGG ACAACTTAGC GCTTTACAAT CGCCAGTAAA TATCACAAGT 
AGCAACAAAT ACAACATCAA AGCTACAAAT TTAACTACTA GTTTACTCCG AGAGTCGATT 
TCTGATTCTA CAACTATGTG TGATACTTTG TCAGACATCA ATTTAACGGT TATGGATGAA 
GATTATCGCA TTGACGGCGA TTCATATTAC GAAGAAGATT CACCAACTTT CATGATCAGC 
CTGGAAAGAA ACATAAAAAA ATGCAATTCA CAATTCAGTC CAAAAAGGTA CATAGGTGAG 
AAATGTTTAA TCTGTGAGGA AAGTATTAGT TCCACATTTA CCGGTGAAAA GGTTGTGGAG 
TCTACGTGTT CACACACTAG CCACTATAAC TGTTATTTGA TGCTATTTGA AACTCTTTAT 
TTCCAAGGCA AGTTTCCTGA GTGTAAGATA TGTGGCGAAG TGAGCAAGCC AAAGGATAAA 
GACATTGTTC CTGAAATGGT ATCCAAACTT CTAACGGGCG CAGGCGCTCA TGATGATGGT 
CCGTCATCCA ATATGCAACA GCAATGGATT GATTTGAAAA CGGCGAGAAG CTTCACAGGT 
GAATTTCCTC AGTTTACACC ACAGGAGCAG CTCATTCGTA CTGCTGATAT TTCATGTGAT 
GGGTTCAGGA CACCACGCTT ATCGAATAGC AATCAATTTG AGGCCGTTAG CTATCTTGAT 
AGCCCATTTT TAAATTCACC ATTCGTTAAT AAAATGGCAA CTACAGACCC TTTTGATTTA 
AGTGATGATG AAAAACTGGA CTGTGATGAT GAAATAGACG AAAGTGCTGC TGAAGTGTGG 
TTTAGTAAAA CGGGTGGTGA ACATGTTATG GTCAGTGTGA AGTTTCAAGA AATGCGGACC 
AGTGATGATT TAGGGGTCTT GCAAGATGTG AATCATGTGG ACCACGAAGA ACTAGAGGAA 
AGGGAAAAGG AATGGAAAAA GAAAATTGAT CAATATATCG AAACAAATGT GGATAAGGAT 
AGCGAATTTG GCAGTTTGAT CTTGTTTGAC AAACTAATGT ATTCTGATGA TGGCGAACAA 
TGGGTAGACA ATAACCTTGT AATATTATTC TCCAAATTTC TAGTTTTGTT TGATTTTGAA 
GAAATGAAGA TCCTGGGTAA GATTCCTAGA GACCAGTTTT ACCAGGTAAT CAAGTTCAAT 
GAGGACGTAC TACTTTGTAG TTTGAAGAGC ACTAATATAC CTGAAATATA CTTGAGGTTC 
AACGAAAACT GCGAAAAGTG GTTGCTTCCC AAATGGAAGT ATTGTCTAGA AAATTCTTCA 
CTGGAGACAC TACCATTAAG TGAGATTGTT TCTACAGTGA AGGAACTCTC TCATGTGAAT 
ATAATAGGCG CTTTAGGAGC GCCGCCTGAT GTTATATCAG CACAATCCCA TGATTCTCGT 
CTACCGTGGA AAAGATTGCA CAGTGATACG CCGCTAAAAC TTATTGTATG TTTGAATTTG 
TCGCATGCTG ATGGGGAACT GTATCGCAAG AGAGTTCTAA AGAGTGTTCA TCAAATATTG 
GATGGATTAA ACACAGACGA TTTACTGGGA ATAGTTGTTG TTGGAAGAGA TGGATCCGGT 
GTAGTAGGCC CATTTGGCAC CTTCATCGGA ATGATAAATA AAAATTGGGA CGGATGGACA 
ACTTTCTTGG ATAATTTAGA AGTGGTTAAT CCAAACGTCT TCCGCGATGA AAAGCAACAG 
TATAAGGTAA CATTACAAAC TTGTGAAAGA TTAGCGTCAA CAAGTGCATA CGTGGATACA 
GATGACCATA TTGCAACTGG ATACGCCAAG CAAATCTTGG TCCTCAACGG GAGTGACGTT 
GTTGACATTG AGCATGATCA GAAATTAAAA AAGGCCTTTG ACCAGTTATC TTACCATTGG 
AGATACGAAA TTTCGCAACG CCGCATGACT CCATTGAACG CTAGCATTAA GCAGTTTTTA 
GAGGAGCTGC ACACTAAGCG TTATCTAGAT GTAACTCTTC GTCTACCACA AGCTACATTC 
GAGCAAGTAT ATTTGGGGGA TATGGCTGCG GGTGAACAAA AAACGCGTCT AATTATGGAT 
GAACACCCGC ATTCTAGTTT GATAGAAATA GAATATTTTG ACCTGGTAAA GCAGCAAAGA 
ATTCATCAGA CCCTGGAAGT TCCCAACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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FAR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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