HAL5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HAL5
Entrez Gene ID	853274
Full Name	protein kinase HAL5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181598.1	NP_012370.1	protein kinase HAL5

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AEL118C	NP_984743.1
Saccharomyces cerevisiae (baker's yeast)	HAL5	NP_012370.1
Kluyveromyces lactis	KLLA0A06820g	XP_451304.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Caffeine sensitivity of the Deltappz1 disruptant is suppressed by disruption of either SAT4 or HAL5.
Title: Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Hal5 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state.

Hal4 and Hal5 are required for control of carbon and nitrogen uptake and metabolism.
Title: Hal4 and Hal5 protein kinases are required for general control of carbon and nitrogen uptake and metabolism.

The protein kinase Hal5p is the high-copy suppressor of lithium-sensitive mutations of genes involved in the sporulation and meiosis as well as the ergosterol biosynthesis in
Title: The protein kinase Hal5p is the high-copy suppressor of lithium-sensitive mutations of genes involved in the sporulation and meiosis as well as the ergosterol biosynthesis in Saccharomyces cerevisiae.

Sat4/Hal4 and Hal5 stabilize the transporter at the plasma membrane under low K+ conditions, preventing its endocytosis and vacuolar degradation.
Title: Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters.

The following HAL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02554	NM_001181598.1 Latest version!	Saccharomyces cerevisiae S288C protein kinase HAL5 (HAL5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAL5 ( NM_001181598.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012370.1 Saccharomyces cerevisiae S288C protein kinase HAL5 (HAL5), partial mRNA.

Price & Availability↑

CloneID	OSi02554
Clone ID Related Accession (Same CDS sequence)	NM_001181598.1
Accession Version	NM_001181598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase HAL5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGAGATG AGAAGCTTTC ACGCCACACA TCTTTGAAGA GGGCCAGATC GCTCTCCGAA 
TCCATTAAGG GTCTATTTAA GCCTTCAGGG ATATCAGGCA GTAATAATGC TGCGGCTCCT 
TCATCTCGTC CCGGTCAGGA CCAAGCACAT TCTCATCAAA CAGCAAGAAT AATAACTAGC 
AACGTATCTT CCCCGTCTAT ATCCCCCGTA CATAGCCCTG TTCTACAAGC GGCTCCTAAG 
CATCACAAAC TGGGCGTCCC TAATATTGCA AAACTCTCGT TGTCACCTAG TAGGGAACCA 
TCTTTGAATT CAGAAAATGA AATGTTTTCA CAAGAATCTT TCATAAGTGA AAAAGATGAA 
GATGAAGCCA ATTTGTTAGA GAGAGAGGAT CTTCAAAATA AAAAGGAAGA GAAAGCTCGT 
GCAAAACATG TACGTTCCAA GGAAGCTTAT GTGCCTCATC ACCGCTATAC AGTTGGTAGC 
GATGAGGTTG AAAGACAACC AAGGGAACGG CTGAAAAACT TCCCACAAAA CGCAGGTTCT 
TCAAACCCTG CAAATAGTAA TGCCAACCAT GTTTTGGATC AGGAAAATAA CTTTTCTATT 
GATGCTATGC TTGATTATGA CGAAGAATCA AAACTTAGAA GGAGAAACAG TCTGGGCGTA 
CGTAATCATA GCAATCGCAC AAGAAGTAGG AAGAACAGTT TGTCCACACC AAGGTCACCA 
CCAATGAAAA ATGGAAATGG TGGAATGAAT TCAAATGCCA CCAACAATGT TGGTAATGGT 
ACAGGAAATA GAATATACAT GAGGGGAAGA AATCACTCAG ACTCTATCAG TGCTTCCAGC 
TTACCGAAAT TTCAAGAAAT CGAATGTAAA TGTATTTTGG ATTTAGGTCA TTTCAAAGTC 
TTTGAAAACG GATACCACGA ACATTCTTTG AGAGTGCTGC CAATTATTAC AAACAATAAA 
AATGTGGATA GTGGCGATGA AAAGGATGCT GACGCTTCTG TAAACAGCGG CGATGATGGT 
GACAATGACA GTGAGGCAAA TATGCATAAA CAAAAATCTG TGTTTTCTTT AAGTGGATTA 
TTCAAATCTC ATAAGGATGG AAACCAACAA CAGCAGCAAC AGCAGCAACA AGAAGAAAAC 
GGCGAACAAA TCAATCTAGA AAAAGCATTT TCAATAATAC CATCCCAGAG ATTCATCAAA 
TCGCAGACTT TAAAGAAATC GAGAACGAGT AACCTTAAAA ATGGCAATAA TGATGAATTG 
ATGAAAAACG ATGGTAAAAA TATTCCGCAA ATCGTGAATC CTAACGCTGC TGTAGGTGTT 
GAAGAGTTGA AGCTAATCAA TGCATTATCG GAAAAAATAC GAAAGGGCCT TAAAAGTGAA 
AATACAAAAG GCAACAATGG CGAAGGCAGA AGCAATAGCA ATAAACAGGA GGATTCTGAT 
GACACAGAGG GCAAGGCAGG AACAACCAAT GACGATACAT CTCATAAACC TTGTTCACAA 
AAATACGGAA AATCTATTGG GGTCGTCGGT GCAGGTGCAT ACGGGGTGGT CAAAATTTGC 
GCAAGGTGCA AGACTGCTAA AGATGTCCTA CCATACAGTA CCTACTCTAA TGGAAAAAAA 
CTATTCTTTG CCGTCAAAGA GCTGAAGCCC AAGCCAGGTG ACCAAATTGA TAAATTTTGC 
ACGAGATTAA CCTCTGAGTT CATTATTGGA CACTCACTGA GCCATCCGCA TTTTGAGGCA 
AATGCGATGA TAGCAGGAAA TGTTTCAAGA ACCACTCCAC CAAAACATGT CTTCAATGCC 
CCAAACATTT TGAAAATTTT GGACCTAATG GAATATAGCA ATTCCTTCGT AGAAGTGATG 
GAATTTTGTG CTTCAGGTGA TTTATATTCG TTGCTAACAA GGAATAACAT CTCAAATGAA 
TCGAATAACG GGAGTTCAAG ATTGATCCAA ACTGTTAAAG AAGGTTCTGG GAGTCCGTTG 
CACCCATTAG AAGCAGACTG TTTTATGAAA CAGCTTTTGA ATGGTGTTCA ATACATGCAT 
GATCATGGTA TAGCACACTG TGATTTGAAA CCCGAAAATA TCCTTTTCCA ACCTAATGGG 
TTGTTAAAAA TTTGTGATTT TGGTACTAGT TCAGTTTTTC AGACTGCCTG GGAGAAACAC 
GTCCACTTCC AGAGTGGAGC CATGGGTTCA GAGCCATATG TGGCACCTGA AGAATTTATA 
AGGGATGCAG AATACGATCC GCGTCTGGTA GATTGTTGGA GTTGTGGTAT TGTTTACTGT 
ACAATGGTCA TGGGACAGTA TCTTTGGAAA ATCGCCATTC CTGAAAAGGA TTCTCTTTTT 
AAATCATTTC TCAGTGAAAT TAAAGATGAT GGACAGTTTT ATCTATTTGA GGAATTAAGA 
CATGTTAGTA GCGAACTGAA TAGGTTGAGG AAAATTGCAC TATATAGAAC GTTCCAGGTC 
GACCCCACGA AGAGAATCAC CATTGAACAA TTACTACAAA GCTCCTGGAT GAGGAAAACA 
AAATGCTGTG TAGTTTATAG ACACTTACAT ACCAAGGTTA GTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012370.1
CDS	1..2568
Translation	MGDEKLSRHTSLKRARSLSESIKGLFKPSGISGSNNAAAPSSRPGQDQAHSHQTARIITSNVSSPSISPVHSPVLQAAPKHHKLGVPNIAKLSLSPSREPSLNSENEMFSQESFISEKDEDEANLLEREDLQNKKEEKARAKHVRSKEAYVPHHRYTVGSDEVERQPRERLKNFPQNAGSSNPANSNANHVLDQENNFSIDAMLDYDEESKLRRRNSLGVRNHSNRTRSRKNSLSTPRSPPMKNGNGGMNSNATNNVGNGTGNRIYMRGRNHSDSISASSLPKFQEIECKCILDLGHFKVFENGYHEHSLRVLPIITNNKNVDSGDEKDADASVNSGDDGDNDSEANMHKQKSVFSLSGLFKSHKDGNQQQQQQQQQEENGEQINLEKAFSIIPSQRFIKSQTLKKSRTSNLKNGNNDELMKNDGKNIPQIVNPNAAVGVEELKLINALSEKIRKGLKSENTKGNNGEGRSNSNKQEDSDDTEGKAGTTNDDTSHKPCSQKYGKSIGVVGAGAYGVVKICARCKTAKDVLPYSTYSNGKKLFFAVKELKPKPGDQIDKFCTRLTSEFIIGHSLSHPHFEANAMIAGNVSRTTPPKHVFNAPNILKILDLMEYSNSFVEVMEFCASGDLYSLLTRNNISNESNNGSSRLIQTVKEGSGSPLHPLEADCFMKQLLNGVQYMHDHGIAHCDLKPENILFQPNGLLKICDFGTSSVFQTAWEKHVHFQSGAMGSEPYVAPEEFIRDAEYDPRLVDCWSCGIVYCTMVMGQYLWKIAIPEKDSLFKSFLSEIKDDGQFYLFEELRHVSSELNRLRKIALYRTFQVDPTKRITIEQLLQSSWMRKTKCCVVYRHLHTKVSK

Target ORF information:

RefSeq Version	NM_001181598.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase HAL5 (HAL5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181598.1

ORF Insert Sequence:

ATGGGAGATG AGAAGCTTTC ACGCCACACA TCTTTGAAGA GGGCCAGATC GCTCTCCGAA 
TCCATTAAGG GTCTATTTAA GCCTTCAGGG ATATCAGGCA GTAATAATGC TGCGGCTCCT 
TCATCTCGTC CCGGTCAGGA CCAAGCACAT TCTCATCAAA CAGCAAGAAT AATAACTAGC 
AACGTATCTT CCCCGTCTAT ATCCCCCGTA CATAGCCCTG TTCTACAAGC GGCTCCTAAG 
CATCACAAAC TGGGCGTCCC TAATATTGCA AAACTCTCGT TGTCACCTAG TAGGGAACCA 
TCTTTGAATT CAGAAAATGA AATGTTTTCA CAAGAATCTT TCATAAGTGA AAAAGATGAA 
GATGAAGCCA ATTTGTTAGA GAGAGAGGAT CTTCAAAATA AAAAGGAAGA GAAAGCTCGT 
GCAAAACATG TACGTTCCAA GGAAGCTTAT GTGCCTCATC ACCGCTATAC AGTTGGTAGC 
GATGAGGTTG AAAGACAACC AAGGGAACGG CTGAAAAACT TCCCACAAAA CGCAGGTTCT 
TCAAACCCTG CAAATAGTAA TGCCAACCAT GTTTTGGATC AGGAAAATAA CTTTTCTATT 
GATGCTATGC TTGATTATGA CGAAGAATCA AAACTTAGAA GGAGAAACAG TCTGGGCGTA 
CGTAATCATA GCAATCGCAC AAGAAGTAGG AAGAACAGTT TGTCCACACC AAGGTCACCA 
CCAATGAAAA ATGGAAATGG TGGAATGAAT TCAAATGCCA CCAACAATGT TGGTAATGGT 
ACAGGAAATA GAATATACAT GAGGGGAAGA AATCACTCAG ACTCTATCAG TGCTTCCAGC 
TTACCGAAAT TTCAAGAAAT CGAATGTAAA TGTATTTTGG ATTTAGGTCA TTTCAAAGTC 
TTTGAAAACG GATACCACGA ACATTCTTTG AGAGTGCTGC CAATTATTAC AAACAATAAA 
AATGTGGATA GTGGCGATGA AAAGGATGCT GACGCTTCTG TAAACAGCGG CGATGATGGT 
GACAATGACA GTGAGGCAAA TATGCATAAA CAAAAATCTG TGTTTTCTTT AAGTGGATTA 
TTCAAATCTC ATAAGGATGG AAACCAACAA CAGCAGCAAC AGCAGCAACA AGAAGAAAAC 
GGCGAACAAA TCAATCTAGA AAAAGCATTT TCAATAATAC CATCCCAGAG ATTCATCAAA 
TCGCAGACTT TAAAGAAATC GAGAACGAGT AACCTTAAAA ATGGCAATAA TGATGAATTG 
ATGAAAAACG ATGGTAAAAA TATTCCGCAA ATCGTGAATC CTAACGCTGC TGTAGGTGTT 
GAAGAGTTGA AGCTAATCAA TGCATTATCG GAAAAAATAC GAAAGGGCCT TAAAAGTGAA 
AATACAAAAG GCAACAATGG CGAAGGCAGA AGCAATAGCA ATAAACAGGA GGATTCTGAT 
GACACAGAGG GCAAGGCAGG AACAACCAAT GACGATACAT CTCATAAACC TTGTTCACAA 
AAATACGGAA AATCTATTGG GGTCGTCGGT GCAGGTGCAT ACGGGGTGGT CAAAATTTGC 
GCAAGGTGCA AGACTGCTAA AGATGTCCTA CCATACAGTA CCTACTCTAA TGGAAAAAAA 
CTATTCTTTG CCGTCAAAGA GCTGAAGCCC AAGCCAGGTG ACCAAATTGA TAAATTTTGC 
ACGAGATTAA CCTCTGAGTT CATTATTGGA CACTCACTGA GCCATCCGCA TTTTGAGGCA 
AATGCGATGA TAGCAGGAAA TGTTTCAAGA ACCACTCCAC CAAAACATGT CTTCAATGCC 
CCAAACATTT TGAAAATTTT GGACCTAATG GAATATAGCA ATTCCTTCGT AGAAGTGATG 
GAATTTTGTG CTTCAGGTGA TTTATATTCG TTGCTAACAA GGAATAACAT CTCAAATGAA 
TCGAATAACG GGAGTTCAAG ATTGATCCAA ACTGTTAAAG AAGGTTCTGG GAGTCCGTTG 
CACCCATTAG AAGCAGACTG TTTTATGAAA CAGCTTTTGA ATGGTGTTCA ATACATGCAT 
GATCATGGTA TAGCACACTG TGATTTGAAA CCCGAAAATA TCCTTTTCCA ACCTAATGGG 
TTGTTAAAAA TTTGTGATTT TGGTACTAGT TCAGTTTTTC AGACTGCCTG GGAGAAACAC 
GTCCACTTCC AGAGTGGAGC CATGGGTTCA GAGCCATATG TGGCACCTGA AGAATTTATA 
AGGGATGCAG AATACGATCC GCGTCTGGTA GATTGTTGGA GTTGTGGTAT TGTTTACTGT 
ACAATGGTCA TGGGACAGTA TCTTTGGAAA ATCGCCATTC CTGAAAAGGA TTCTCTTTTT 
AAATCATTTC TCAGTGAAAT TAAAGATGAT GGACAGTTTT ATCTATTTGA GGAATTAAGA 
CATGTTAGTA GCGAACTGAA TAGGTTGAGG AAAATTGCAC TATATAGAAC GTTCCAGGTC 
GACCCCACGA AGAGAATCAC CATTGAACAA TTACTACAAA GCTCCTGGAT GAGGAAAACA 
AAATGCTGTG TAGTTTATAG ACACTTACAT ACCAAGGTTA GTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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HAL5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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