PHO90 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PHO90
Entrez Gene ID	853241
Full Name	SPX domain-containing inorganic phosphate transporter
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181631.1	NP_012337.1	SPX domain-containing inorganic phosphate transporter

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PHO90	NP_012337.1
Eremothecium gossypii	AGOS_AFR628C	NP_986175.2
Saccharomyces cerevisiae (baker's yeast)	PHO87	NP_009966.2
Kluyveromyces lactis	KLLA0C03454g	XP_452350.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pho90 represent non-redundant P(i) transporters, which are tuned by the integration of multiple nutrient signalling mechanisms in order to adjust P(i)-transport capacity to the general nutritional status of the environment.
Title: Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae.

SPX domain of Pho90 limits the phosphate-uptake velocity, suppresses phosphate efflux and affects the regulation of the phosphate signal transduction pathway.
Title: The SPX domain of the yeast low-affinity phosphate transporter Pho90 regulates transport activity.

The following PHO90 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHO90 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02526	NM_001181631.1 Latest version!	Saccharomyces cerevisiae S288C SPX domain-containing inorganic phosphate transporter (PHO90), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHO90 ( NM_001181631.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012337.1 Saccharomyces cerevisiae S288C SPX domain-containing inorganic phosphate transporter (PHO90), partial mRNA.

Price & Availability↑

CloneID	OSi02526
Clone ID Related Accession (Same CDS sequence)	NM_001181631.1
Accession Version	NM_001181631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SPX domain-containing inorganic phosphate transporter
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGATTTT CACACTTCTT GAAGTACAAT GCTGTCCCAG AATGGCAAAA CCATTATATG 
GACTACAGCG AGCTGAAAAA TCTTATTTAC ACGCTACAAA CAGATGAACT TCAGGTTGGT 
GATAACGAAG AAGGATTTGG CGCAGGAAAG AGCTCTAACA TTACAGATAG GTTCAAAAAC 
AAGTTTTCTT TTAAAAATGC GAAGGAAGAT ACGTCTTCCG GTATGAACAA AGATGCAGGC 
ATCGTTGAGG AAACCATCGA GTTGCGAGAG TTGCCTACTG CTCAGACGGT CGCTGCCAAA 
CCTTCTCCTT TCAGAAGAAT GAAGGAAAAG ATATTTTACA AAAGAAGGTC GTCTTCCGCA 
TCGTCCGTCT CCTCCACGGC CAACGAAAAT CTGCAATTAG ACACTTATGA TACGTTTGTT 
GGTGATTTAA CAGCTGAAAA ACAGAAAGTA GATGATTTTT ATAAGAGGAC AGAAGCGAAG 
TTCTACGACA AATTTGACGC GCTGGTGAAG GACCTGAAGA AAATCGGAGT TATAGAATAC 
GATATCGACG ATGATACTCT GTTTAACGAA CCGATTGCCA GCACAAATGA CGAAGTTCCC 
CCACTAGACT TGGATGATGA CGAAGACGAC GACGAATTTT ACGATGATCA ATCTAATATT 
GAAGATAATA CTGCTTTGCT GCATCATTCG CAGTATAACA TTAAGTCTCA GAAAAAATCG 
CTGTTGAAGA AGTCGATCGT AAACCTATAT ATCGATCTTT GCCAGTTGAA GTCGTTCATC 
GAATTGAACC GCATTGGGTT TGCAAAAATT ACAAAGAAAT CAGACAAAGT TCTTCACTTG 
AATACAAGAA CCGAACTGAT CGAATCGGAG CAGTTTTTCA AAGACACATA TGCATTCCAG 
GCAGAAACGA TCGAATTGCT AAATTCCAAA ATTTCCCAGC TAGTCACATT TTATGCGCGC 
ATCACTGACC GGCCTCATAA TATCTCGCAT AGCAAGCAAG AGTTGAAATC CTACCTGCAT 
GACCACATTG TTTGGGAAAG AAGTAACACT TGGAAAGACA TGTTGGGACT GCTATCGCAA 
GCTGACGAGT TGACACCAAA GGAAACAGAA TATAATGCAA ATAAGCTGGT AGGCAAGTTA 
GATTTGGAAT ACTACAGATG GCCACTACCC AGACCGATAA ACTTAAAATT CACTAGTATA 
AACAACGTTG CACTACCGAA ATTATTTTTC ACCAAGAAAG CATACAAGAT TTACTTTATT 
ATTCTAGTCA CTGGACTCTT GTTAGGAATA AAGACCTTCA ACGACGCTGC TCAGCACCGC 
TGCATGGCCC TTGTCGAGTG TGTCGCCTTT TTGTGGGCTA GTGAGGCCAT CCCATTACAC 
ATTACAGCAT TCCTTGTACC ACTACTTGTA GTCCTTTTCA AAGTCCTAAA AACCTCCGAC 
GGGGCTATAA TGAGTGCTGC AAGCGCTTCA TCAGAAATTT TGGCCGCCAT GTGGTCTTCT 
ACAATTATGA TTCTGCTGGC AGGTTTTACT TTGGGTGAAG TACTTGCACA ATATAACATC 
GCCAAAGTTC TTGCCTCGTG GTTGTTGGCC TTCGCTGGTT GTAAACCCAG AAACGTTCTT 
TTAATGGCAA TGTGTGTCGT GTTCTTCCTA TCAATGTGGA TTTCCAATGT CGCAGCACCT 
GTTCTAACAT ATTCGTTGTT ATCTCCCCTA TTGGATGCCA TGGATGCAGA TAGCCCATTT 
GCGCAAGCAT TGGTGTTAGG TGTAGCGTTG GCTGCAAATA TCGGTGGTAT GTCTTCACCA 
ATCTCTTCAC CTCAAAACAT CATTTCCATG TCGTACTTGA AACCCTATGG TATTGGCTGG 
GGCCAATTCT TTGCTGTTGC ATTGCCATCT GGTATCCTGG CCATGCTTTT GGTTTGGATT 
TTATTGTTCA CTACTTTCAA GATGAATAAG ACCAAATTGG AAAAATTTAA GCCTATTAAG 
ACGAAATTCA CAGTTAAGCA GTATTATATC ATTACTGTCA CTGTGGCCAC TATTTTGTTG 
TGGTGTGTGG AAAGCCAGAT TGAAGGTGCT TTTGGGTCAT CAGGTCAAAT TGCAATCATT 
CCCATCGTTT TGTTTTTTGG TACCGGATTA CTATCAACAC AAGATTTAAA TGCCTTTCCG 
TGGTCAATCG TTATTTTGGC AATGGGAGGT ATTGCTTTGG GGAAGGCCGT CTCATCCTCG 
GGTTTGCTAT CAACCATTGC AAAAGCATTA CAAAAGAAAA TTGAGAATGA TGGTGTTTTT 
GCCATTCTAT GTATTTTCGG TATCCTGATG TTGGTTGTGG GTACTTTCGT CTCGCATACA 
GTATCCGCTA TTATCATCAT TCCCTTGGTG CAAGAAGTTG GTGACAAGCT TGGCAACCCC 
AAAGCTGCTC CTATCCTTGT TTTCGGTTGT GCATTATTGT CATCCTGTGG TATGGGACTA 
GCTTCTTCGG GGTTCCCCAA TGTCACTGCC ATCTCTAAAG TAGATAGAAA GGGCGATCGC 
TATTTAAGTG TCATGACTTT CTTAACGAGA GGTGTTCCAG CTTCTATTTT GGCTTTCCTA 
TGTGTCATTA CTCTAGGTTA TGGTATCATG GCATCTGTTG TCAAGGGTAA CGCAACCTCT 
GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012337.1
CDS	1..2646
Translation	MRFSHFLKYNAVPEWQNHYMDYSELKNLIYTLQTDELQVGDNEEGFGAGKSSNITDRFKNKFSFKNAKEDTSSGMNKDAGIVEETIELRELPTAQTVAAKPSPFRRMKEKIFYKRRSSSASSVSSTANENLQLDTYDTFVGDLTAEKQKVDDFYKRTEAKFYDKFDALVKDLKKIGVIEYDIDDDTLFNEPIASTNDEVPPLDLDDDEDDDEFYDDQSNIEDNTALLHHSQYNIKSQKKSLLKKSIVNLYIDLCQLKSFIELNRIGFAKITKKSDKVLHLNTRTELIESEQFFKDTYAFQAETIELLNSKISQLVTFYARITDRPHNISHSKQELKSYLHDHIVWERSNTWKDMLGLLSQADELTPKETEYNANKLVGKLDLEYYRWPLPRPINLKFTSINNVALPKLFFTKKAYKIYFIILVTGLLLGIKTFNDAAQHRCMALVECVAFLWASEAIPLHITAFLVPLLVVLFKVLKTSDGAIMSAASASSEILAAMWSSTIMILLAGFTLGEVLAQYNIAKVLASWLLAFAGCKPRNVLLMAMCVVFFLSMWISNVAAPVLTYSLLSPLLDAMDADSPFAQALVLGVALAANIGGMSSPISSPQNIISMSYLKPYGIGWGQFFAVALPSGILAMLLVWILLFTTFKMNKTKLEKFKPIKTKFTVKQYYIITVTVATILLWCVESQIEGAFGSSGQIAIIPIVLFFGTGLLSTQDLNAFPWSIVILAMGGIALGKAVSSSGLLSTIAKALQKKIENDGVFAILCIFGILMLVVGTFVSHTVSAIIIIPLVQEVGDKLGNPKAAPILVFGCALLSSCGMGLASSGFPNVTAISKVDRKGDRYLSVMTFLTRGVPASILAFLCVITLGYGIMASVVKGNATSA

Target ORF information:

RefSeq Version	NM_001181631.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SPX domain-containing inorganic phosphate transporter (PHO90), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181631.1

ORF Insert Sequence:

ATGAGATTTT CACACTTCTT GAAGTACAAT GCTGTCCCAG AATGGCAAAA CCATTATATG 
GACTACAGCG AGCTGAAAAA TCTTATTTAC ACGCTACAAA CAGATGAACT TCAGGTTGGT 
GATAACGAAG AAGGATTTGG CGCAGGAAAG AGCTCTAACA TTACAGATAG GTTCAAAAAC 
AAGTTTTCTT TTAAAAATGC GAAGGAAGAT ACGTCTTCCG GTATGAACAA AGATGCAGGC 
ATCGTTGAGG AAACCATCGA GTTGCGAGAG TTGCCTACTG CTCAGACGGT CGCTGCCAAA 
CCTTCTCCTT TCAGAAGAAT GAAGGAAAAG ATATTTTACA AAAGAAGGTC GTCTTCCGCA 
TCGTCCGTCT CCTCCACGGC CAACGAAAAT CTGCAATTAG ACACTTATGA TACGTTTGTT 
GGTGATTTAA CAGCTGAAAA ACAGAAAGTA GATGATTTTT ATAAGAGGAC AGAAGCGAAG 
TTCTACGACA AATTTGACGC GCTGGTGAAG GACCTGAAGA AAATCGGAGT TATAGAATAC 
GATATCGACG ATGATACTCT GTTTAACGAA CCGATTGCCA GCACAAATGA CGAAGTTCCC 
CCACTAGACT TGGATGATGA CGAAGACGAC GACGAATTTT ACGATGATCA ATCTAATATT 
GAAGATAATA CTGCTTTGCT GCATCATTCG CAGTATAACA TTAAGTCTCA GAAAAAATCG 
CTGTTGAAGA AGTCGATCGT AAACCTATAT ATCGATCTTT GCCAGTTGAA GTCGTTCATC 
GAATTGAACC GCATTGGGTT TGCAAAAATT ACAAAGAAAT CAGACAAAGT TCTTCACTTG 
AATACAAGAA CCGAACTGAT CGAATCGGAG CAGTTTTTCA AAGACACATA TGCATTCCAG 
GCAGAAACGA TCGAATTGCT AAATTCCAAA ATTTCCCAGC TAGTCACATT TTATGCGCGC 
ATCACTGACC GGCCTCATAA TATCTCGCAT AGCAAGCAAG AGTTGAAATC CTACCTGCAT 
GACCACATTG TTTGGGAAAG AAGTAACACT TGGAAAGACA TGTTGGGACT GCTATCGCAA 
GCTGACGAGT TGACACCAAA GGAAACAGAA TATAATGCAA ATAAGCTGGT AGGCAAGTTA 
GATTTGGAAT ACTACAGATG GCCACTACCC AGACCGATAA ACTTAAAATT CACTAGTATA 
AACAACGTTG CACTACCGAA ATTATTTTTC ACCAAGAAAG CATACAAGAT TTACTTTATT 
ATTCTAGTCA CTGGACTCTT GTTAGGAATA AAGACCTTCA ACGACGCTGC TCAGCACCGC 
TGCATGGCCC TTGTCGAGTG TGTCGCCTTT TTGTGGGCTA GTGAGGCCAT CCCATTACAC 
ATTACAGCAT TCCTTGTACC ACTACTTGTA GTCCTTTTCA AAGTCCTAAA AACCTCCGAC 
GGGGCTATAA TGAGTGCTGC AAGCGCTTCA TCAGAAATTT TGGCCGCCAT GTGGTCTTCT 
ACAATTATGA TTCTGCTGGC AGGTTTTACT TTGGGTGAAG TACTTGCACA ATATAACATC 
GCCAAAGTTC TTGCCTCGTG GTTGTTGGCC TTCGCTGGTT GTAAACCCAG AAACGTTCTT 
TTAATGGCAA TGTGTGTCGT GTTCTTCCTA TCAATGTGGA TTTCCAATGT CGCAGCACCT 
GTTCTAACAT ATTCGTTGTT ATCTCCCCTA TTGGATGCCA TGGATGCAGA TAGCCCATTT 
GCGCAAGCAT TGGTGTTAGG TGTAGCGTTG GCTGCAAATA TCGGTGGTAT GTCTTCACCA 
ATCTCTTCAC CTCAAAACAT CATTTCCATG TCGTACTTGA AACCCTATGG TATTGGCTGG 
GGCCAATTCT TTGCTGTTGC ATTGCCATCT GGTATCCTGG CCATGCTTTT GGTTTGGATT 
TTATTGTTCA CTACTTTCAA GATGAATAAG ACCAAATTGG AAAAATTTAA GCCTATTAAG 
ACGAAATTCA CAGTTAAGCA GTATTATATC ATTACTGTCA CTGTGGCCAC TATTTTGTTG 
TGGTGTGTGG AAAGCCAGAT TGAAGGTGCT TTTGGGTCAT CAGGTCAAAT TGCAATCATT 
CCCATCGTTT TGTTTTTTGG TACCGGATTA CTATCAACAC AAGATTTAAA TGCCTTTCCG 
TGGTCAATCG TTATTTTGGC AATGGGAGGT ATTGCTTTGG GGAAGGCCGT CTCATCCTCG 
GGTTTGCTAT CAACCATTGC AAAAGCATTA CAAAAGAAAA TTGAGAATGA TGGTGTTTTT 
GCCATTCTAT GTATTTTCGG TATCCTGATG TTGGTTGTGG GTACTTTCGT CTCGCATACA 
GTATCCGCTA TTATCATCAT TCCCTTGGTG CAAGAAGTTG GTGACAAGCT TGGCAACCCC 
AAAGCTGCTC CTATCCTTGT TTTCGGTTGT GCATTATTGT CATCCTGTGG TATGGGACTA 
GCTTCTTCGG GGTTCCCCAA TGTCACTGCC ATCTCTAAAG TAGATAGAAA GGGCGATCGC 
TATTTAAGTG TCATGACTTT CTTAACGAGA GGTGTTCCAG CTTCTATTTT GGCTTTCCTA 
TGTGTCATTA CTCTAGGTTA TGGTATCATG GCATCTGTTG TCAAGGGTAA CGCAACCTCT 
GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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PHO90 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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