ORF » Species Summary » Saccharomyces cerevisiae S288C » DIE2 cDNA ORF clone

DIE2 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following DIE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02443 NM_001181356.2
Latest version!		 Saccharomyces cerevisiae S288C dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase (DIE2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    DIE2 ( NM_001181356.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011743.2 Saccharomyces cerevisiae S288C dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase (DIE2), partial mRNA.
    Price & Availability↑
    CloneID OSi02443
    Clone ID Related Accession (Same CDS sequence) NM_001181356.2
    Accession Version NM_001181356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Apr 26, 2011 this sequence version replaced NM_001181356.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGATGCAA AGAAAAATAC TGGTGAAGCC AATAATGATG TATTGGAAGA GGAAGCAGCG 
    ATTCAGTTGA TTGCACCAGG TATAGCGAGA AACTTAACAC AAGAAGTCAT TACCGGTATC 
    TTCTGTAATG TAGTAATATA TCCTCTTCTT CTAATATATT TTGTCTTAAC TTTCCGTTAC 
    ATGACTACAA ATATTGTACC TTATGAATTT ATAGATGAAA AGTTTCACGT TGGCCAAACT 
    TTGACCTATT TAAAAGGTAA ATGGACTCAA TGGGACCCTA AAATAACAAC CCCCCCTGGA 
    ATCTATATTT TGGGTTTAAT TAATTATTAT TGTATAAAGC CCATATTCAA GTCGTGGAGC 
    ACACTAACCA TACTAAGATT AGTTAATTTA CTTGGAGGGA TTATCGTTTT CCCCATTTTA 
    GTACTCAGGC CAATATTCCT TTTTAATGCC TTAGGTTTTT GGCCCGTTTC ATTAATGAGC 
    TTCCCATTGA TGACAACTTA TTATTACTTA TTTTACACCG ATGTGTGGTC TACAATTCTG 
    ATATTGCAAT CACTCAGCTG CGTGTTAACA TTACCGTTTG GCCCCGTGAA GAGTATATGG 
    TTGAGTGCTT TTTTTGCTGG TGTAAGCTGT CTCTTCAGAC AAACGAATAT CATTTGGACG 
    GGATTTATAA TGATTCTTGC TGTTGAACGC CCAGCCATTT TACAAAAACA GTTCAACACG 
    CATACGTTCA ATAACTATTT GAAATTATTC ATTCATGCTA TTGACGATTT CTCCAACCTT 
    GTTCTGCCCT ATATGATAAA TTTTGTTTTG TTCTTCATTT ATCTGATTTG GAACAGATCC 
    ATAACACTAG GTGATAAGTC CAGCCACTCA GCAGGCCTGC ATATAGTACA AATATTCTAT 
    TGTTTCACAT TCATCACCGT TTTCAGTTTA CCTATATGGA TATCTCGTAA TTTTATGAAG 
    TTGTACAAAT TAAGAATAAA ACGGAAGCCA GTACAAACTT TTTTTGAATT TATTGGTATT 
    ATGCTAATCA TAAGATACTT TACAAAAGTT CACCCATTTT TGTTAGCAGA TAATAGACAC 
    TACACGTTCT ACCTCTTCAG AAGATTGATA GGCAACAAAA GTAGACTAAT CAAGTACTTT 
    TTTATGACAC CAATCTATCA TTTCTCCACT TTCGCATACC TTGAAGTGAT GAGACCCAAC 
    CAGCTCACCT TTCACCCCAT CACTCCACTC CCCATCAAAG AACCTGTACA TTTACCCATA 
    CAACTGACAC ATGTATCGTG GACGGCCTTA ATAACGTGCA CCATGGTAAC TATCGTTCCA 
    TCACCCTTAT TCGAACCTCG TTACTATATA TTGCCCTATT TCTTTTGGAG AATATTTATT 
    ACCTGCAGCT GCGAACCTTT GATAAAGGAT TTAAAACCTG CTAAAGAAGG CGAAAACCCA 
    ATTACCATAA GCAGTACGAA AAGACTGTTC ATGGAATTTT TATGGTTCAT GCTCTTCAAT 
    GTCGTTACTT TAGTTATCTT TTCCAAGGTC TCATTTCCTT GGACCACTGA GCCCTATTTG 
    CAAAGAATAA TATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011743.2
    CDS1..1578
    Translation

    Target ORF information:

    RefSeq Version NM_001181356.2
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase (DIE2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGATGCAA AGAAAAATAC TGGTGAAGCC AATAATGATG TATTGGAAGA GGAAGCAGCG 
    ATTCAGTTGA TTGCACCAGG TATAGCGAGA AACTTAACAC AAGAAGTCAT TACCGGTATC 
    TTCTGTAATG TAGTAATATA TCCTCTTCTT CTAATATATT TTGTCTTAAC TTTCCGTTAC 
    ATGACTACAA ATATTGTACC TTATGAATTT ATAGATGAAA AGTTTCACGT TGGCCAAACT 
    TTGACCTATT TAAAAGGTAA ATGGACTCAA TGGGACCCTA AAATAACAAC CCCCCCTGGA 
    ATCTATATTT TGGGTTTAAT TAATTATTAT TGTATAAAGC CCATATTCAA GTCGTGGAGC 
    ACACTAACCA TACTAAGATT AGTTAATTTA CTTGGAGGGA TTATCGTTTT CCCCATTTTA 
    GTACTCAGGC CAATATTCCT TTTTAATGCC TTAGGTTTTT GGCCCGTTTC ATTAATGAGC 
    TTCCCATTGA TGACAACTTA TTATTACTTA TTTTACACCG ATGTGTGGTC TACAATTCTG 
    ATATTGCAAT CACTCAGCTG CGTGTTAACA TTACCGTTTG GCCCCGTGAA GAGTATATGG 
    TTGAGTGCTT TTTTTGCTGG TGTAAGCTGT CTCTTCAGAC AAACGAATAT CATTTGGACG 
    GGATTTATAA TGATTCTTGC TGTTGAACGC CCAGCCATTT TACAAAAACA GTTCAACACG 
    CATACGTTCA ATAACTATTT GAAATTATTC ATTCATGCTA TTGACGATTT CTCCAACCTT 
    GTTCTGCCCT ATATGATAAA TTTTGTTTTG TTCTTCATTT ATCTGATTTG GAACAGATCC 
    ATAACACTAG GTGATAAGTC CAGCCACTCA GCAGGCCTGC ATATAGTACA AATATTCTAT 
    TGTTTCACAT TCATCACCGT TTTCAGTTTA CCTATATGGA TATCTCGTAA TTTTATGAAG 
    TTGTACAAAT TAAGAATAAA ACGGAAGCCA GTACAAACTT TTTTTGAATT TATTGGTATT 
    ATGCTAATCA TAAGATACTT TACAAAAGTT CACCCATTTT TGTTAGCAGA TAATAGACAC 
    TACACGTTCT ACCTCTTCAG AAGATTGATA GGCAACAAAA GTAGACTAAT CAAGTACTTT 
    TTTATGACAC CAATCTATCA TTTCTCCACT TTCGCATACC TTGAAGTGAT GAGACCCAAC 
    CAGCTCACCT TTCACCCCAT CACTCCACTC CCCATCAAAG AACCTGTACA TTTACCCATA 
    CAACTGACAC ATGTATCGTG GACGGCCTTA ATAACGTGCA CCATGGTAAC TATCGTTCCA 
    TCACCCTTAT TCGAACCTCG TTACTATATA TTGCCCTATT TCTTTTGGAG AATATTTATT 
    ACCTGCAGCT GCGAACCTTT GATAAAGGAT TTAAAACCTG CTAAAGAAGG CGAAAACCCA 
    ATTACCATAA GCAGTACGAA AAGACTGTTC ATGGAATTTT TATGGTTCAT GCTCTTCAAT 
    GTCGTTACTT TAGTTATCTT TTCCAAGGTC TCATTTCCTT GGACCACTGA GCCCTATTTG 
    CAAAGAATAA TATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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