AMA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	AMA1
Entrez Gene ID	853140
Full Name	Ama1p
Synonyms	SPO70
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181354.3	NP_011741.5	Ama1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E15863g	XP_454665.1
Eremothecium gossypii	AGOS_ADL135C	NP_983961.2
Saccharomyces cerevisiae (baker's yeast)	AMA1	NP_011741.5

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic dissection of prospore membrane closure finds SPS1 and SPO77 act in parallel to a previously described pathway of prospore membrane closure that involves AMA1, an activator of the meiotic anaphase promoting complex.
Title: Timely Closure of the Prospore Membrane Requires SPS1 and SPO77 in Saccharomyces cerevisiae.

Study shows that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor.
Title: Meiotic prophase requires proteolysis of M phase regulators mediated by the meiosis-specific APC/CAma1.

These results demonstrate that APC(Ama1) functions to coordinate the exit from meiosis II with cytokinesis.
Title: The anaphase promoting complex targeting subunit Ama1 links meiotic exit to cytokinesis during sporulation in Saccharomyces cerevisiae.

Cdc28 and Ama1 function in a pathway to trigger Smk1-dependent steps in spore morphogenesis.
Title: The Ama1-directed anaphase-promoting complex regulates the Smk1 mitogen-activated protein kinase during meiosis in yeast.

Chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C-Ama1.
Title: The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1.

The following AMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02442	NM_001181354.3 Latest version!	Saccharomyces cerevisiae S288C Ama1p (AMA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMA1 ( NM_001181354.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011741.5 Saccharomyces cerevisiae S288C Ama1p (AMA1), partial mRNA.

Price & Availability↑

CloneID	OSi02442
Clone ID Related Accession (Same CDS sequence)	NM_001181354.3
Accession Version	NM_001181354.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ama1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181354.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTACTC CCCATTTATA TCACAGATAT AACTCCAAAA GCTCTAACAA AAATATCAAT 
TCGAGCGGAA ATTCTACAGA GGTTGACCGT TTCATTCCAA AATCAGTTTC GAGAAATGCT 
TATAAGTCTA TTCCAATGTT AAATGGGTTT GACATAAGTT ACTCAGAACT TTGCGAGAAA 
TCTCCTTCAC CTGAGCGGCT ATCATCCCCT GAATTCTTTA ACGAACTGCG AAACACCGGG 
CATTACGAAT CAATCAGTAC AACAAATGAG TTTTCCATGT CAAGTATCTC TAGCTCTTCT 
GAGAGTCAAG TTACCAGAAG TGGAAGTGCA AGAGCTTCAA GAAACGATTA TTCAAAATTA 
ACTAAAGAGC AAAAGGACCA TAGAAAGAAT ATTGCACATT CTCTTGGTTT TCAATTGCCC 
GACCGTGTCT TTACGTTTGA GACCACAAGT GCTGAAATTC TGGAAAAGAA TAAAGCCATC 
AAAAATTGCT TTGGACCAGG AAGTTGCGCC GAAATAAGAT CAACCTTTGA CTTTAGTACA 
CTCTCGCCCG ATGTTGCCCG TTATTATATC GCTAATTCTA ACGCAAGATC AGCTTCCCCA 
CAAAGACAAA TCCAAAGACC TGCTAAACGA GTAAAGTCAC ATATTCCATA CCGTGTATTG 
GATGCACCTT GCCTGAGAAA TGATTTTTAT TCGAACTTAA TTTCTTGGTC AAGAACCACA 
AACAACGTTT TGGTGGGACT GGGTTGCTCC GTATACATCT GGTCTGAGAA AGAAGGAGCG 
GTATCTATAT TGGACCATCA ATACTTAAGT GAAAAGAGGG ATTTAGTGAC ATGCGTCTCA 
TTCTGTCCTT ACAATACTTA TTTTATTGTT GGTACAAAAT TTGGCCGTAT TCTTCTTTAT 
GATCAAAAAG AATTTTTTCA TTCCAGCAAC ACAAACGAAA AGGAACCAGT ATTTGTTTTC 
CAAACTGAAT CCTTCAAAGG AATATGTTGC CTAGAATGGT TCAAACCAGG TGAAATTTGC 
AAATTTTATG TTGGAGAGGA AAATGGCAAT GTGAGCCTCT TTGAAATAAA GAGTTTGCAT 
TTCCCCATAA AAAACTGGAG TAAAAGACAA AAACTCGAAG ATGAAAATTT AATAGGATTG 
AAACTTCATT CCACTTATCA AGCTCAGGCA CAGCAAGTCT GTGGTATTTC TCTGAATGAA 
CATGCAAACC TGCTTGCAGT CGGCGGCAAT GATAATTCTT GTAGCTTATG GGATATTTCC 
GACTTGGATA AACCTATTAA AAAGTTTGTT CTTCCACATA AAGCGGCTGT GAAGGCCATT 
GCCTTTTGTC CTTGGTCGAA GTCTTTGTTG GCTACGGGTG GTGGAAGCAA AGACAGATGT 
ATTAAGTTTT GGCATACATC GACAGGCACA TTGTTGGATG AGATTTGCAC CTCGGGACAG 
GTGACGTCTT TGATATGGTC GTTGAGACAT AAACAGATTG TTGCTACTTT TGGATTCGGT 
GATACCAAGA ATCCTGTATT AATAACACTA TACTCATATC CAAAATTATC AAAACTACTC 
GAAGTTAGGT CGCCCAACCC TTTGCGTGTA CTGAGCGCAG TAATCTCTCC AAGTTCCATG 
GCAATTTGCG TTGCCACTAA CGATGAAACG ATAAGATTTT ATGAACTGTG GAACGATAAG 
GAGGAAATAA TTAATGAAAT TCAAGAGAGT GGGATTTATG GATCTAATAT TATAGAATAT 
ATGGAGGGTA TCGAAACAAC ACATAACAAA AGAATAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011741.5
CDS	1..1782
Translation	MATPHLYHRYNSKSSNKNINSSGNSTEVDRFIPKSVSRNAYKSIPMLNGFDISYSELCEKSPSPERLSSPEFFNELRNTGHYESISTTNEFSMSSISSSSESQVTRSGSARASRNDYSKLTKEQKDHRKNIAHSLGFQLPDRVFTFETTSAEILEKNKAIKNCFGPGSCAEIRSTFDFSTLSPDVARYYIANSNARSASPQRQIQRPAKRVKSHIPYRVLDAPCLRNDFYSNLISWSRTTNNVLVGLGCSVYIWSEKEGAVSILDHQYLSEKRDLVTCVSFCPYNTYFIVGTKFGRILLYDQKEFFHSSNTNEKEPVFVFQTESFKGICCLEWFKPGEICKFYVGEENGNVSLFEIKSLHFPIKNWSKRQKLEDENLIGLKLHSTYQAQAQQVCGISLNEHANLLAVGGNDNSCSLWDISDLDKPIKKFVLPHKAAVKAIAFCPWSKSLLATGGGSKDRCIKFWHTSTGTLLDEICTSGQVTSLIWSLRHKQIVATFGFGDTKNPVLITLYSYPKLSKLLEVRSPNPLRVLSAVISPSSMAICVATNDETIRFYELWNDKEEIINEIQESGIYGSNIIEYMEGIETTHNKRIR

Target ORF information:

RefSeq Version	NM_001181354.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ama1p (AMA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181354.3

ORF Insert Sequence:

ATGGCTACTC CCCATTTATA TCACAGATAT AACTCCAAAA GCTCTAACAA AAATATCAAT 
TCGAGCGGAA ATTCTACAGA GGTTGACCGT TTCATTCCAA AATCAGTTTC GAGAAATGCT 
TATAAGTCTA TTCCAATGTT AAATGGGTTT GACATAAGTT ACTCAGAACT TTGCGAGAAA 
TCTCCTTCAC CTGAGCGGCT ATCATCCCCT GAATTCTTTA ACGAACTGCG AAACACCGGG 
CATTACGAAT CAATCAGTAC AACAAATGAG TTTTCCATGT CAAGTATCTC TAGCTCTTCT 
GAGAGTCAAG TTACCAGAAG TGGAAGTGCA AGAGCTTCAA GAAACGATTA TTCAAAATTA 
ACTAAAGAGC AAAAGGACCA TAGAAAGAAT ATTGCACATT CTCTTGGTTT TCAATTGCCC 
GACCGTGTCT TTACGTTTGA GACCACAAGT GCTGAAATTC TGGAAAAGAA TAAAGCCATC 
AAAAATTGCT TTGGACCAGG AAGTTGCGCC GAAATAAGAT CAACCTTTGA CTTTAGTACA 
CTCTCGCCCG ATGTTGCCCG TTATTATATC GCTAATTCTA ACGCAAGATC AGCTTCCCCA 
CAAAGACAAA TCCAAAGACC TGCTAAACGA GTAAAGTCAC ATATTCCATA CCGTGTATTG 
GATGCACCTT GCCTGAGAAA TGATTTTTAT TCGAACTTAA TTTCTTGGTC AAGAACCACA 
AACAACGTTT TGGTGGGACT GGGTTGCTCC GTATACATCT GGTCTGAGAA AGAAGGAGCG 
GTATCTATAT TGGACCATCA ATACTTAAGT GAAAAGAGGG ATTTAGTGAC ATGCGTCTCA 
TTCTGTCCTT ACAATACTTA TTTTATTGTT GGTACAAAAT TTGGCCGTAT TCTTCTTTAT 
GATCAAAAAG AATTTTTTCA TTCCAGCAAC ACAAACGAAA AGGAACCAGT ATTTGTTTTC 
CAAACTGAAT CCTTCAAAGG AATATGTTGC CTAGAATGGT TCAAACCAGG TGAAATTTGC 
AAATTTTATG TTGGAGAGGA AAATGGCAAT GTGAGCCTCT TTGAAATAAA GAGTTTGCAT 
TTCCCCATAA AAAACTGGAG TAAAAGACAA AAACTCGAAG ATGAAAATTT AATAGGATTG 
AAACTTCATT CCACTTATCA AGCTCAGGCA CAGCAAGTCT GTGGTATTTC TCTGAATGAA 
CATGCAAACC TGCTTGCAGT CGGCGGCAAT GATAATTCTT GTAGCTTATG GGATATTTCC 
GACTTGGATA AACCTATTAA AAAGTTTGTT CTTCCACATA AAGCGGCTGT GAAGGCCATT 
GCCTTTTGTC CTTGGTCGAA GTCTTTGTTG GCTACGGGTG GTGGAAGCAA AGACAGATGT 
ATTAAGTTTT GGCATACATC GACAGGCACA TTGTTGGATG AGATTTGCAC CTCGGGACAG 
GTGACGTCTT TGATATGGTC GTTGAGACAT AAACAGATTG TTGCTACTTT TGGATTCGGT 
GATACCAAGA ATCCTGTATT AATAACACTA TACTCATATC CAAAATTATC AAAACTACTC 
GAAGTTAGGT CGCCCAACCC TTTGCGTGTA CTGAGCGCAG TAATCTCTCC AAGTTCCATG 
GCAATTTGCG TTGCCACTAA CGATGAAACG ATAAGATTTT ATGAACTGTG GAACGATAAG 
GAGGAAATAA TTAATGAAAT TCAAGAGAGT GGGATTTATG GATCTAATAT TATAGAATAT 
ATGGAGGGTA TCGAAACAAC ACATAACAAA AGAATAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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AMA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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