BUB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	853100
Full Name	protein kinase BUB1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181317.3	NP_011704.3	protein kinase BUB1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F22605g	XP_456090.1
Eremothecium gossypii	AGOS_AGR315C	NP_986981.2
Neurospora crassa	NCU10043	XP_957960.2
Saccharomyces cerevisiae (baker's yeast)	BUB1	NP_011704.3
Magnaporthe oryzae (rice blast fungus)	MGG_14329	XP_003710704.1
Arabidopsis thaliana (thale cress)	AT2G20635	NP_179656.4
Oryza sativa (rice)	Os07g0508500	NP_001059745.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bub1 mutant cells fail to maintain a prolonged checkpoint arrest in the presence of tensionless attachment.
Title: Premature Silencing of the Spindle Assembly Checkpoint Is Prevented by the Bub1-H2A-Sgo1-PP2A Axis in

Data indicate that spindle checkpoint factors Bub1 and Bub2 are recruited to the site of DNA damage.
Title: Spindle Checkpoint Factors Bub1 and Bub2 Promote DNA Double-Strand Break Repair by Nonhomologous End Joining.

Bub1 and Sgo1 act as a rheostat to regulate the chromatin spring and maintain force balance. Through histone H2A S121 phosphorylation and recruitment of Sgo1, Bub1 kinase softens the chromatin spring in response to changes in microtubule dynamics.
Title: Bub1 kinase and Sgo1 modulate pericentric chromatin in response to altered microtubule dynamics.

propose that Bub1, Sgo1, and Mps1 facilitate chromosome biorientation independently of the Aurora B-mediated pathway at the budding yeast kinetochore and that both pathways are required for the efficient turnover of syntelic attachments
Title: Bub1, Sgo1, and Mps1 mediate a distinct pathway for chromosome biorientation in budding yeast.

Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest.
Title: Bub1-mediated adaptation of the spindle checkpoint.

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02407	NM_001181317.3 Latest version!	Saccharomyces cerevisiae S288C protein kinase BUB1 (BUB1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( NM_001181317.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011704.3 Saccharomyces cerevisiae S288C protein kinase BUB1 (BUB1), partial mRNA.

Price & Availability↑

CloneID	OSi02407
Clone ID Related Accession (Same CDS sequence)	NM_001181317.3
Accession Version	NM_001181317.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase BUB1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181317.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATTTAG ATTTAGGTTC TACTGTACGA GGCTATGAAT CAGATAAAGA CACATTTCCG 
CAATCGAAAG GCGTTAGCTC ATCTCAAAAA GAGCAGCATA GTCAACTGAA TCAGACAAAA 
ATAGCTTATG AACAGAGACT TCTAAATGAT TTAGAGGATA TGGATGATCC TTTAGATTTG 
TTTTTAGATT ATATGATATG GATTAGCACC AGCTATATTG AAGTAGATTC TGAATCAGGT 
CAAGAAGTTC TCCGAAGTAC AATGGAAAGA TGCTTGATTT ACATACAAGA TATGGAAACC 
TATAGGAACG ATCCCAGATT TTTAAAAATT TGGATTTGGT ATATAAATTT GTTTCTTAGC 
AACAACTTCC ACGAGAGTGA AAATACTTTT AAATATATGT TTAACAAGGG AATCGGAACA 
AAGCTTTCGT TATTTTATGA AGAGTTCTCC AAGCTACTAG AAAATGCTCA ATTTTTTCTA 
GAAGCAAAGG TGTTATTAGA ACTAGGGGCT GAAAACAATT GCAGGCCTTA CAACAGACTA 
TTAAGGTCAT TAAGCAATTA TGAAGACAGA CTCCGAGAAA TGAACATCGT AGAAAACCAG 
AACAGTGTAC CTGATTCAAG GGAAAGATTG AAGGGAAGGT TAATTTATAG AACAGCACCA 
TTTTTCATTC GTAAATTTTT AACCTCATCA TTGATGACTG ATGACAAGGA AAATCGTGCG 
AACCTGAACT CTAATGTTGG AGTTGGTAAG TCTGCTCCTA ACGTATATCA AGATTCTATA 
GTGGTTGCTG ACTTTAAGTC AGAAACAGAG AGGCTTAATT TGAATAGCTC TAAACAGCCC 
TCTAACCAAC GCTTGAAGAA TGGAAATAAA AAAACTTCAA TATATGCAGA TCAAAAGCAA 
TCTAACAATC CAGTCTACAA ACTAATCAAT ACTCCGGGAC GGAAACCTGA ACGGATAGTT 
TTTAATTTTA ATCTAATATA TCCAGAAAAT GATGAGGAGT TCAACACCGA AGAAATATTG 
GCAATGATTA AGGGACTTTA CAAGGTACAG CGCAGAGGTA AGAAGCACAC CGAAGATTAT 
ACAAGTGACA AAAATAGGAA GAAACGTAAG CTCGATGTGC TGGTAGAGAG ACGGCAAGAC 
TTACCTTCAT CACAGCCACC CGTTGTCCCC AAATCTACGC GAATTGAGGT GTTTAAAGAT 
GACGATAATC CCAGCCAGAG CACACACCAT AAAAATACAC AGGTACAAGT GCAAACGACA 
ACGTCTATTT TACCTTTGAA GCCTGTGGTT GATGGAAACC TCGCGCATGA GACACCAGTG 
AAGCCCTCAC TAACATCCAA CGCTTCCCGT TCACCTACAG TAACAGCTTT TTCAAAAGAC 
GCTATAAATG AGGTCTTTTC CATGTTCAAT CAACATTATT CAACCCCGGG TGCGCTATTA 
GATGGTGATG ATACCACTAC AAGTAAATTC AACGTCTTCG AAAATTTTAC CCAAGAATTT 
ACAGCAAAGA ATATTGAAGA TTTAACTGAA GTTAAAGATC CTAAACAAGA GACGGTGTCA 
CAACAAACTA CCTCAACAAA TGAAACCAAC GATCGATATG AAAGATTGTC GAATAGTAGC 
ACACGGCCAG AAAAGGCAGA CTACATGACG CCTATAAAAG AAACTACTGA AACAGATGTG 
GTACCTATAA TCCAGACACC TAAGGAACAA ATAAGGACAG AAGATAAGAA ATCGGGGGAT 
AATACAGAAA CACAAACTCA ATTAACAAGC ACAACCATTC AAAGTTCTCC ATTTCTCACA 
CAACCTGAAC CACAAGCAGA AAAGCTTTTA CAAACTGCAG AACATTCGGA AAAGAGTAAA 
GAGCATTACC CAACGATCAT ACCTCCCTTC ACTAAAATAA AAAATCAACC GCCTGTCATT 
ATTGAAAACC CACTTAGTAA TAATTTAAGA GCGAAGTTTT TATCCGAAAT TTCGCCCCCA 
TTATTCCAAT ATAACACCTT TTATAACTAC AACCAAGAAT TGAAAATGAG TTCTCTATTG 
AAAAAAATCC ATAGGGTATC CCGCAACGAA AACAAGAACC CAATTGTTGA CTTCAAGAAG 
ACGGGTGATT TATATTGTAT ACGCGGAGAA TTAGGCGAAG GAGGTTATGC CACTGTATAT 
CTAGCAGAAT CTAGTCAAGG ACATCTGCGA GCGTTGAAAG TAGAAAAACC TGCAAGTGTA 
TGGGAATATT ACATAATGAG CCAAGTGGAG TTTAGGTTGA GGAAGAGCAC AATATTAAAG 
TCAATAATCA ACGCTAGTGC TTTACACTTG TTCCTTGATG AGAGCTACCT TGTTTTAAAC 
TACGCCAGTC AAGGTACGGT CTTGGATTTA ATTAATTTAC AACGGGAAAA GGCAATTGAC 
GGCAACGGAA TAATGGATGA ATATTTGTGC ATGTTTATCA CTGTCGAGCT AATGAAAGTA 
CTTGAAAAGA TACATGAAGT GGGAATAATA CATGGCGATT TGAAACCAGA TAACTGCATG 
ATCAGATTAG AAAAACCAGG CGAACCCCTG GGTGCTCATT ATATGCGCAA TGGAGAGGAT 
GGCTGGGAAA ACAAGGGAAT CTATTTAATA GATTTCGGTA GATCATTTGA TATGACACTG 
TTACCACCTG GAACGAAATT CAAATCAAAT TGGAAGGCTG ATCAGCAAGA TTGCTGGGAA 
ATGCGTGCGG GCAAGCCATG GAGTTATGAA GCGGATTATT ATGGATTAGC AGGCGTCATA 
CATTCGATGC TTTTTGGAAA ATTCATAGAA ACAATCCAGC TGCAGAATGG ACGATGCAAA 
TTGAAGAACC CATTCAAAAG GTATTGGAAA AAAGAAATAT GGGGCGTTAT ATTTGATTTA 
CTGTTAAATA GCGGTCAAGC TTCCAATCAA GCTTTGCCGA TGACTGAAAA GATTGTTGAA 
ATAAGGAATT TGATAGAAAG CCATCTGGAA CAGCACGCAG AAAATCACTT AAGAAACGTA 
ATTCTAAGCA TTGAAGAGGA GTTATCACAT TTTCAATATA AGGGGAAACC GTCAAGGAGA 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011704.3
CDS	1..3066
Translation	MNLDLGSTVRGYESDKDTFPQSKGVSSSQKEQHSQLNQTKIAYEQRLLNDLEDMDDPLDLFLDYMIWISTSYIEVDSESGQEVLRSTMERCLIYIQDMETYRNDPRFLKIWIWYINLFLSNNFHESENTFKYMFNKGIGTKLSLFYEEFSKLLENAQFFLEAKVLLELGAENNCRPYNRLLRSLSNYEDRLREMNIVENQNSVPDSRERLKGRLIYRTAPFFIRKFLTSSLMTDDKENRANLNSNVGVGKSAPNVYQDSIVVADFKSETERLNLNSSKQPSNQRLKNGNKKTSIYADQKQSNNPVYKLINTPGRKPERIVFNFNLIYPENDEEFNTEEILAMIKGLYKVQRRGKKHTEDYTSDKNRKKRKLDVLVERRQDLPSSQPPVVPKSTRIEVFKDDDNPSQSTHHKNTQVQVQTTTSILPLKPVVDGNLAHETPVKPSLTSNASRSPTVTAFSKDAINEVFSMFNQHYSTPGALLDGDDTTTSKFNVFENFTQEFTAKNIEDLTEVKDPKQETVSQQTTSTNETNDRYERLSNSSTRPEKADYMTPIKETTETDVVPIIQTPKEQIRTEDKKSGDNTETQTQLTSTTIQSSPFLTQPEPQAEKLLQTAEHSEKSKEHYPTIIPPFTKIKNQPPVIIENPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKKIHRVSRNENKNPIVDFKKTGDLYCIRGELGEGGYATVYLAESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKSTILKSIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVELMKVLEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSFDMTLLPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQNGRCKLKNPFKRYWKKEIWGVIFDLLLNSGQASNQALPMTEKIVEIRNLIESHLEQHAENHLRNVILSIEEELSHFQYKGKPSRRF

Target ORF information:

RefSeq Version	NM_001181317.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase BUB1 (BUB1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181317.3

ORF Insert Sequence:

ATGAATTTAG ATTTAGGTTC TACTGTACGA GGCTATGAAT CAGATAAAGA CACATTTCCG 
CAATCGAAAG GCGTTAGCTC ATCTCAAAAA GAGCAGCATA GTCAACTGAA TCAGACAAAA 
ATAGCTTATG AACAGAGACT TCTAAATGAT TTAGAGGATA TGGATGATCC TTTAGATTTG 
TTTTTAGATT ATATGATATG GATTAGCACC AGCTATATTG AAGTAGATTC TGAATCAGGT 
CAAGAAGTTC TCCGAAGTAC AATGGAAAGA TGCTTGATTT ACATACAAGA TATGGAAACC 
TATAGGAACG ATCCCAGATT TTTAAAAATT TGGATTTGGT ATATAAATTT GTTTCTTAGC 
AACAACTTCC ACGAGAGTGA AAATACTTTT AAATATATGT TTAACAAGGG AATCGGAACA 
AAGCTTTCGT TATTTTATGA AGAGTTCTCC AAGCTACTAG AAAATGCTCA ATTTTTTCTA 
GAAGCAAAGG TGTTATTAGA ACTAGGGGCT GAAAACAATT GCAGGCCTTA CAACAGACTA 
TTAAGGTCAT TAAGCAATTA TGAAGACAGA CTCCGAGAAA TGAACATCGT AGAAAACCAG 
AACAGTGTAC CTGATTCAAG GGAAAGATTG AAGGGAAGGT TAATTTATAG AACAGCACCA 
TTTTTCATTC GTAAATTTTT AACCTCATCA TTGATGACTG ATGACAAGGA AAATCGTGCG 
AACCTGAACT CTAATGTTGG AGTTGGTAAG TCTGCTCCTA ACGTATATCA AGATTCTATA 
GTGGTTGCTG ACTTTAAGTC AGAAACAGAG AGGCTTAATT TGAATAGCTC TAAACAGCCC 
TCTAACCAAC GCTTGAAGAA TGGAAATAAA AAAACTTCAA TATATGCAGA TCAAAAGCAA 
TCTAACAATC CAGTCTACAA ACTAATCAAT ACTCCGGGAC GGAAACCTGA ACGGATAGTT 
TTTAATTTTA ATCTAATATA TCCAGAAAAT GATGAGGAGT TCAACACCGA AGAAATATTG 
GCAATGATTA AGGGACTTTA CAAGGTACAG CGCAGAGGTA AGAAGCACAC CGAAGATTAT 
ACAAGTGACA AAAATAGGAA GAAACGTAAG CTCGATGTGC TGGTAGAGAG ACGGCAAGAC 
TTACCTTCAT CACAGCCACC CGTTGTCCCC AAATCTACGC GAATTGAGGT GTTTAAAGAT 
GACGATAATC CCAGCCAGAG CACACACCAT AAAAATACAC AGGTACAAGT GCAAACGACA 
ACGTCTATTT TACCTTTGAA GCCTGTGGTT GATGGAAACC TCGCGCATGA GACACCAGTG 
AAGCCCTCAC TAACATCCAA CGCTTCCCGT TCACCTACAG TAACAGCTTT TTCAAAAGAC 
GCTATAAATG AGGTCTTTTC CATGTTCAAT CAACATTATT CAACCCCGGG TGCGCTATTA 
GATGGTGATG ATACCACTAC AAGTAAATTC AACGTCTTCG AAAATTTTAC CCAAGAATTT 
ACAGCAAAGA ATATTGAAGA TTTAACTGAA GTTAAAGATC CTAAACAAGA GACGGTGTCA 
CAACAAACTA CCTCAACAAA TGAAACCAAC GATCGATATG AAAGATTGTC GAATAGTAGC 
ACACGGCCAG AAAAGGCAGA CTACATGACG CCTATAAAAG AAACTACTGA AACAGATGTG 
GTACCTATAA TCCAGACACC TAAGGAACAA ATAAGGACAG AAGATAAGAA ATCGGGGGAT 
AATACAGAAA CACAAACTCA ATTAACAAGC ACAACCATTC AAAGTTCTCC ATTTCTCACA 
CAACCTGAAC CACAAGCAGA AAAGCTTTTA CAAACTGCAG AACATTCGGA AAAGAGTAAA 
GAGCATTACC CAACGATCAT ACCTCCCTTC ACTAAAATAA AAAATCAACC GCCTGTCATT 
ATTGAAAACC CACTTAGTAA TAATTTAAGA GCGAAGTTTT TATCCGAAAT TTCGCCCCCA 
TTATTCCAAT ATAACACCTT TTATAACTAC AACCAAGAAT TGAAAATGAG TTCTCTATTG 
AAAAAAATCC ATAGGGTATC CCGCAACGAA AACAAGAACC CAATTGTTGA CTTCAAGAAG 
ACGGGTGATT TATATTGTAT ACGCGGAGAA TTAGGCGAAG GAGGTTATGC CACTGTATAT 
CTAGCAGAAT CTAGTCAAGG ACATCTGCGA GCGTTGAAAG TAGAAAAACC TGCAAGTGTA 
TGGGAATATT ACATAATGAG CCAAGTGGAG TTTAGGTTGA GGAAGAGCAC AATATTAAAG 
TCAATAATCA ACGCTAGTGC TTTACACTTG TTCCTTGATG AGAGCTACCT TGTTTTAAAC 
TACGCCAGTC AAGGTACGGT CTTGGATTTA ATTAATTTAC AACGGGAAAA GGCAATTGAC 
GGCAACGGAA TAATGGATGA ATATTTGTGC ATGTTTATCA CTGTCGAGCT AATGAAAGTA 
CTTGAAAAGA TACATGAAGT GGGAATAATA CATGGCGATT TGAAACCAGA TAACTGCATG 
ATCAGATTAG AAAAACCAGG CGAACCCCTG GGTGCTCATT ATATGCGCAA TGGAGAGGAT 
GGCTGGGAAA ACAAGGGAAT CTATTTAATA GATTTCGGTA GATCATTTGA TATGACACTG 
TTACCACCTG GAACGAAATT CAAATCAAAT TGGAAGGCTG ATCAGCAAGA TTGCTGGGAA 
ATGCGTGCGG GCAAGCCATG GAGTTATGAA GCGGATTATT ATGGATTAGC AGGCGTCATA 
CATTCGATGC TTTTTGGAAA ATTCATAGAA ACAATCCAGC TGCAGAATGG ACGATGCAAA 
TTGAAGAACC CATTCAAAAG GTATTGGAAA AAAGAAATAT GGGGCGTTAT ATTTGATTTA 
CTGTTAAATA GCGGTCAAGC TTCCAATCAA GCTTTGCCGA TGACTGAAAA GATTGTTGAA 
ATAAGGAATT TGATAGAAAG CCATCTGGAA CAGCACGCAG AAAATCACTT AAGAAACGTA 
ATTCTAAGCA TTGAAGAGGA GTTATCACAT TTTCAATATA AGGGGAAACC GTCAAGGAGA 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BUB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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