TFG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TFG1
Entrez Gene ID	853098
Full Name	transcription factor IIF subunit TFG1
Synonyms	RAP74,SSU71
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181315.1	NP_011702.1	transcription factor IIF subunit TFG1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AAL149C	NP_982393.1
Saccharomyces cerevisiae (baker's yeast)	TFG1	NP_011702.1
Kluyveromyces lactis	KLLA0F15411g	XP_455772.1
Schizosaccharomyces pombe (fission yeast)	tfg1	NP_588467.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Monitoring elongation complexes containing RNAPII and combinations of TFIIF (SSU71) and TFIIS to mechanical loads, found that both factors are independently capable of restoring arrested RNAPII to productive elongation.
Title: Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms.

TFIIF mainly prevents Pol II from pause entering, whereas TFIIS assists with pause recovery, and quantitatively described these effects on the kinetics of transcription elongation by Pol II.
Title: Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.

Tfg1 C-terminal domain interacts with double-stranded DNA oligonucleotides.
Title: Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2.

TFIIF stimulates formation of the first two phosphodiester bonds and dramatically stabilizes a short RNA-DNA hybrid in the RNA polymerase II (RNAPII) active center.
Title: Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization.

Evidence that the Tfg1/Tfg2 dimer interface of TFIIF is located in proximity to the B-finger of RNA polymerase II (TFIIB) near the active center of RNAP II where the TFIIB-TFIIF-RNAP II interface plays a key role in transcription start site selection.
Title: Evidence that the Tfg1/Tfg2 dimer interface of TFIIF lies near the active center of the RNA polymerase II initiation complex.

The following TFG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TFG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02405	NM_001181315.1 Latest version!	Saccharomyces cerevisiae S288C transcription factor IIF subunit TFG1 (TFG1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TFG1 ( NM_001181315.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011702.1 Saccharomyces cerevisiae S288C transcription factor IIF subunit TFG1 (TFG1), partial mRNA.

Price & Availability↑

CloneID	OSi02405
Clone ID Related Accession (Same CDS sequence)	NM_001181315.1
Accession Version	NM_001181315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	transcription factor IIF subunit TFG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCAGAC GCAATCCACC AGGCAGTAGA AACGGGGGAG GTCCGACGAA TGCCTCTCCC 
TTCATTAAAA GAGACAGAAT GAGAAGAAAT TTCCTGAGGA TGAGAATGGG TCAAAACGGC 
AGCAATTCTA GTTCCCCAGG CGTACCAAAT GGAGACAACA GCAGGGGATC TCTTGTCAAA 
AAGGATGATC CTGAGTATGC TGAAGAGAGA GAGAAAATGC TTTTGCAAAT CGGTGTCGAG 
GCCGATGCTG GCAGGAGCAA TGTTAAAGTA AAGGACGAAG ATCCTAATGA ATATAATGAA 
TTTCCCTTAA GAGCTATTCC AAAGGAGGAT TTAGAAAACA TGAGGACACA TCTTTTGAAG 
TTTCAGAGTA AAAAAAAGAT AAACCCTGTA ACAGACTTCC ATCTTCCCGT AAGACTGCAT 
AGGAAGGATA CGAGAAACTT GCAGTTTCAG CTAACAAGAG CAGAAATTGT TCAAAGGCAA 
AAAGAAATTT CTGAGTATAA AAAAAAAGCA GAGCAAGAAA GAAGTACTCC TAACTCGGGT 
GGTATGAATA AATCAGGAAC GGTCTCGCTC AATAATACTG TTAAAGATGG GTCTCAGACA 
CCTACTGTTG ATTCAGTTAC TAAAGACAAC ACGGCCAACG GTGTGAACTC CTCCATTCCT 
ACTGTAACTG GCTCTTCTGT CCCTCCTGCT AGTCCTACTA CTGTATCCGC CGTTGAAAGT 
AATGGACTGT CAAATGGTTC AACTAGTGCA GCTAACGGTT TGGATGGCAA TGCATCTACA 
GCGAATCTAG CTAACGGAAG GCCGTTAGTG ACAAAACTAG AGGATGCAGG CCCAGCTGAA 
GACCCTACTA AAGTTGGAAT GGTCAAGTAC GACGGAAAAG AAGTGACTAA CGAACCGGAA 
TTTGAAGAAG GAACTATGGA TCCACTAGCA GATGTGGCAC CAGATGGAGG CGGTAGGGCC 
AAACGAGGAA ATTTGAGAAG GAAAACTCGT CAATTAAAAG TTCTTGATGA GAACGCCAAA 
AAGCTGAGAT TTGAGGAATT TTATCCATGG GTTATGGAAG ATTTTGATGG GTACAATACA 
TGGGTGGGTT CTTATGAAGC AGGTAACTCA GATTCTTATG TCTTGTTAAG TGTAGAAGAT 
GATGGAAGTT TTACCATGAT TCCTGCTGAT AAGGTTTATA AGTTTACCGC AAGAAATAAA 
TATGCTACTT TGACCATTGA CGAAGCAGAA AAAAGAATGG ACAAAAAAAG TGGTGAAGTG 
CCACGTTGGT TGATGAAGCA TTTGGATAAT ATAGGTACAA CTACAACTAG ATATGACAGA 
ACAAGAAGAA AGCTTAAAGC CGTCGCTGAT CAACAAGCTA TGGATGAAGA TGATCGTGAT 
GACAATTCAG AAGTAGAGTT AGATTATGAT GAAGAGTTTG CCGATGATGA GGAGGCACCT 
ATTATAGATG GTAATGAACA AGAAAATAAA GAATCAGAAC AAAGAATCAA GAAGGAGATG 
CTGCAAGCTA ATGCGATGGG GCTTCGTGAT GAAGAAGCTC CTTCCGAAAA CGAAGAAGAT 
GAGTTATTTG GGGAAAAGAA GATTGATGAG GATGGTGAAA GAATTAAGAA GGCATTACAA 
AAGACAGAAC TTGCAGCACT ATACTCATCG GATGAAAATG AGATCAACCC ATACTTATCT 
GAATCCGATA TTGAAAACAA AGAGAATGAA TCGCCCGTTA AAAAGGAAGA AGATTCGGAC 
ACTCTATCCA AATCCAAGAG ATCTTCTCCA AAGAAACAGC AAAAGAAAGC TACAAATGCG 
CATGTGCATA AAGAGCCAAC TTTGAGGGTG AAAAGTATTA AAAACTGTGT CATTATCTTG 
AAGGGGGATA AGAAAATACT GAAAAGCTTC CCAGAGGGAG AATGGAATCC ACAGACGACA 
AAAGCTGTAG ATAGTAGTAA TAATGCATCG AATACAGTGC CTTCGCCAAT CAAACAAGAG 
GAAGGCTTGA ATTCTACAGT GGCAGAAAGG GAAGAAACTC CAGCGCCTAC AATAACAGAA 
AAAGATATCA TCGAGGCTAT TGGTGATGGC AAAGTCAATA TCAAAGAGTT TGGAAAGTTC 
ATCAGAAGAA AATATCCTGG CGCAGAGAAT AAAAAGCTAA TGTTTGCCAT TGTAAAAAAA 
TTGTGTCGAA AGGTTGGCAA TGACCACATG GAATTAAAGA AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011702.1
CDS	1..2208
Translation	MSRRNPPGSRNGGGPTNASPFIKRDRMRRNFLRMRMGQNGSNSSSPGVPNGDNSRGSLVKKDDPEYAEEREKMLLQIGVEADAGRSNVKVKDEDPNEYNEFPLRAIPKEDLENMRTHLLKFQSKKKINPVTDFHLPVRLHRKDTRNLQFQLTRAEIVQRQKEISEYKKKAEQERSTPNSGGMNKSGTVSLNNTVKDGSQTPTVDSVTKDNTANGVNSSIPTVTGSSVPPASPTTVSAVESNGLSNGSTSAANGLDGNASTANLANGRPLVTKLEDAGPAEDPTKVGMVKYDGKEVTNEPEFEEGTMDPLADVAPDGGGRAKRGNLRRKTRQLKVLDENAKKLRFEEFYPWVMEDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYKFTARNKYATLTIDEAEKRMDKKSGEVPRWLMKHLDNIGTTTTRYDRTRRKLKAVADQQAMDEDDRDDNSEVELDYDEEFADDEEAPIIDGNEQENKESEQRIKKEMLQANAMGLRDEEAPSENEEDELFGEKKIDEDGERIKKALQKTELAALYSSDENEINPYLSESDIENKENESPVKKEEDSDTLSKSKRSSPKKQQKKATNAHVHKEPTLRVKSIKNCVIILKGDKKILKSFPEGEWNPQTTKAVDSSNNASNTVPSPIKQEEGLNSTVAEREETPAPTITEKDIIEAIGDGKVNIKEFGKFIRRKYPGAENKKLMFAIVKKLCRKVGNDHMELKKE

Target ORF information:

RefSeq Version	NM_001181315.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C transcription factor IIF subunit TFG1 (TFG1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181315.1

ORF Insert Sequence:

ATGTCCAGAC GCAATCCACC AGGCAGTAGA AACGGGGGAG GTCCGACGAA TGCCTCTCCC 
TTCATTAAAA GAGACAGAAT GAGAAGAAAT TTCCTGAGGA TGAGAATGGG TCAAAACGGC 
AGCAATTCTA GTTCCCCAGG CGTACCAAAT GGAGACAACA GCAGGGGATC TCTTGTCAAA 
AAGGATGATC CTGAGTATGC TGAAGAGAGA GAGAAAATGC TTTTGCAAAT CGGTGTCGAG 
GCCGATGCTG GCAGGAGCAA TGTTAAAGTA AAGGACGAAG ATCCTAATGA ATATAATGAA 
TTTCCCTTAA GAGCTATTCC AAAGGAGGAT TTAGAAAACA TGAGGACACA TCTTTTGAAG 
TTTCAGAGTA AAAAAAAGAT AAACCCTGTA ACAGACTTCC ATCTTCCCGT AAGACTGCAT 
AGGAAGGATA CGAGAAACTT GCAGTTTCAG CTAACAAGAG CAGAAATTGT TCAAAGGCAA 
AAAGAAATTT CTGAGTATAA AAAAAAAGCA GAGCAAGAAA GAAGTACTCC TAACTCGGGT 
GGTATGAATA AATCAGGAAC GGTCTCGCTC AATAATACTG TTAAAGATGG GTCTCAGACA 
CCTACTGTTG ATTCAGTTAC TAAAGACAAC ACGGCCAACG GTGTGAACTC CTCCATTCCT 
ACTGTAACTG GCTCTTCTGT CCCTCCTGCT AGTCCTACTA CTGTATCCGC CGTTGAAAGT 
AATGGACTGT CAAATGGTTC AACTAGTGCA GCTAACGGTT TGGATGGCAA TGCATCTACA 
GCGAATCTAG CTAACGGAAG GCCGTTAGTG ACAAAACTAG AGGATGCAGG CCCAGCTGAA 
GACCCTACTA AAGTTGGAAT GGTCAAGTAC GACGGAAAAG AAGTGACTAA CGAACCGGAA 
TTTGAAGAAG GAACTATGGA TCCACTAGCA GATGTGGCAC CAGATGGAGG CGGTAGGGCC 
AAACGAGGAA ATTTGAGAAG GAAAACTCGT CAATTAAAAG TTCTTGATGA GAACGCCAAA 
AAGCTGAGAT TTGAGGAATT TTATCCATGG GTTATGGAAG ATTTTGATGG GTACAATACA 
TGGGTGGGTT CTTATGAAGC AGGTAACTCA GATTCTTATG TCTTGTTAAG TGTAGAAGAT 
GATGGAAGTT TTACCATGAT TCCTGCTGAT AAGGTTTATA AGTTTACCGC AAGAAATAAA 
TATGCTACTT TGACCATTGA CGAAGCAGAA AAAAGAATGG ACAAAAAAAG TGGTGAAGTG 
CCACGTTGGT TGATGAAGCA TTTGGATAAT ATAGGTACAA CTACAACTAG ATATGACAGA 
ACAAGAAGAA AGCTTAAAGC CGTCGCTGAT CAACAAGCTA TGGATGAAGA TGATCGTGAT 
GACAATTCAG AAGTAGAGTT AGATTATGAT GAAGAGTTTG CCGATGATGA GGAGGCACCT 
ATTATAGATG GTAATGAACA AGAAAATAAA GAATCAGAAC AAAGAATCAA GAAGGAGATG 
CTGCAAGCTA ATGCGATGGG GCTTCGTGAT GAAGAAGCTC CTTCCGAAAA CGAAGAAGAT 
GAGTTATTTG GGGAAAAGAA GATTGATGAG GATGGTGAAA GAATTAAGAA GGCATTACAA 
AAGACAGAAC TTGCAGCACT ATACTCATCG GATGAAAATG AGATCAACCC ATACTTATCT 
GAATCCGATA TTGAAAACAA AGAGAATGAA TCGCCCGTTA AAAAGGAAGA AGATTCGGAC 
ACTCTATCCA AATCCAAGAG ATCTTCTCCA AAGAAACAGC AAAAGAAAGC TACAAATGCG 
CATGTGCATA AAGAGCCAAC TTTGAGGGTG AAAAGTATTA AAAACTGTGT CATTATCTTG 
AAGGGGGATA AGAAAATACT GAAAAGCTTC CCAGAGGGAG AATGGAATCC ACAGACGACA 
AAAGCTGTAG ATAGTAGTAA TAATGCATCG AATACAGTGC CTTCGCCAAT CAAACAAGAG 
GAAGGCTTGA ATTCTACAGT GGCAGAAAGG GAAGAAACTC CAGCGCCTAC AATAACAGAA 
AAAGATATCA TCGAGGCTAT TGGTGATGGC AAAGTCAATA TCAAAGAGTT TGGAAAGTTC 
ATCAGAAGAA AATATCCTGG CGCAGAGAAT AAAAAGCTAA TGTTTGCCAT TGTAAAAAAA 
TTGTGTCGAA AGGTTGGCAA TGACCACATG GAATTAAAGA AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TFG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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