UBR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UBR1
Entrez Gene ID	853096
Full Name	E3 ubiquitin-protein ligase UBR1
Synonyms	PTR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181313.1	NP_011700.1	E3 ubiquitin-protein ligase UBR1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AAL146W	NP_982396.1
Kluyveromyces lactis	KLLA0F15334g	XP_455768.1
Saccharomyces cerevisiae (baker's yeast)	UBR1	NP_011700.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In addition to the E3 ubiquitin ligase Ubr1, we identified the prefoldin chaperone subunit Gim3 as an important quality control factor. We found that Gim3 interacted with the Guk1 mutant allele and propose that prefoldin promotes the degradation of the unstable model substrate by maintaining the solubility of the misfolded protein
Title: Prefoldin Promotes Proteasomal Degradation of Cytosolic Proteins with Missense Mutations by Maintaining Substrate Solubility.

The major ubiquitin ligase targeting the superfluous Fas2 subunit to the proteasome is Ubr1. The ubiquitin-conjugating enzymes Ubc2 and Ubc4 assist the degradation process.
Title: Quality control of a cytoplasmic protein complex: chaperone motors and the ubiquitin-proteasome system govern the fate of orphan fatty acid synthase subunit Fas2 of yeast.

Ubr1 Is Present at the endoplasmic reticulum membrane of wild-type cells and becomes a ligase for ER-associated protein degradation of Ste6* under heat and ethanol stress.
Title: Previously unknown role for the ubiquitin ligase Ubr1 in endoplasmic reticulum-associated protein degradation.

Data show that ubiquitin-protein ligases Ubr1 and Ubr2 have opposing roles in Ste11DeltaNK444R-GFP aggregation.
Title: A network of ubiquitin ligases is important for the dynamics of misfolded protein aggregates in yeast.

Study shows that the RING-type Ubr1 E3 and the HECT-type Ufd4 E3 interact, both physically and functionally.
Title: The N-end rule pathway is mediated by a complex of the RING-type Ubr1 and HECT-type Ufd4 ubiquitin ligases.

The following UBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02403	NM_001181313.1 Latest version!	Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase UBR1 (UBR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBR1 ( NM_001181313.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011700.1 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase UBR1 (UBR1), partial mRNA.

Price & Availability↑

CloneID	OSi02403
Clone ID Related Accession (Same CDS sequence)	NM_001181313.1
Accession Version	NM_001181313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	E3 ubiquitin-protein ligase UBR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCGTTG CTGATGATGA TTTAGGATCT TTACAAGGTC ACATTAGGAG AACACTGAGG 
TCTATTCATA ACCTCCCCTA TTTTAGGTAT ACGAGAGGTC CTACTGAAAG GGCTGACATG 
AGCAGAGCCC TTAAAGAGTT CATTTACAGA TATCTATACT TTGTCATTTC TAACAGCGGA 
GAGAACTTAC CTACTTTATT CAATGCTCAT CCAAAACAAA AATTATCTAA CCCAGAGCTT 
ACTGTTTTTC CTGACAGTTT AGAAGATGCT GTGGATATTG ATAAGATAAC ATCTCAACAA 
ACTATTCCGT TTTATAAGAT AGATGAATCC AGAATAGGAG ACGTCCATAA ACATACCGGA 
AGAAATTGTG GGAGGAAATT CAAAATAGGG GAACCCTTGT ATAGGTGTCA TGAGTGTGGT 
TGCGATGATA CTTGTGTGCT TTGTATTCAT TGTTTTAATC CAAAAGATCA TGTGAATCAT 
CATGTTTGTA CCGATATATG TACTGAATTC ACTAGTGGAA TTTGTGACTG TGGAGATGAA 
GAAGCTTGGA ATTCTCCACT TCATTGCAAA GCTGAAGAAC AAGAAAACGA TATATCGGAA 
GATCCCGCCA CAAATGCCGA CATAAAGGAA GAAGATGTAT GGAATGATTC AGTGAATATA 
GCATTAGTGG AGTTGGTTCT TGCAGAAGTG TTTGACTATT TTATCGATGT TTTTAACCAG 
AATATCGAAC CTCTACCCAC AATACAGAAA GATATCACTA TCAAATTAAG AGAGATGACA 
CAACAGGGAA AAATGTATGA AAGAGCGCAG TTTTTAAACG ATTTAAAATA TGAAAACGAT 
TATATGTTTG ACGGAACAAC AACAGCAAAG ACGAGCCCCT CTAATAGCCC TGAGGCATCT 
CCATCGCTTG CGAAAATCGA TCCCGAAAAT TACACGGTAA TAATATATAA CGATGAATAT 
CATAACTATT CGCAGGCTAC GACTGCCTTG AGACAGGGGG TTCCAGATAA CGTACACATT 
GATCTATTAA CATCCAGGAT TGACGGAGAA GGTCGAGCAA TGTTGAAATG TTCACAAGAT 
TTATCATCTG TCTTAGGTGG TTTTTTTGCT GTTCAAACGA ATGGATTAAG TGCCACTTTA 
ACATCGTGGT CAGAATATCT TCATCAAGAA ACATGCAAGT ATATCATCCT TTGGATTACA 
CACTGTTTAA ACATACCAAA CTCCTCCTTC CAAACCACTT TTAGGAACAT GATGGGTAAA 
ACCCTGTGTT CAGAATACTT AAATGCAACA GAATGTCGTG ATATGACACC GGTGGTGGAG 
AAATATTTCA GCAACAAGTT CGATAAGAAT GATCCCTACA GATACATCGA TTTATCTATC 
CTTGCAGATG GCAATCAAAT TCCTTTGGGT CATCACAAAA TTCTACCCGA ATCAAGCACC 
CATTCACTTT CACCATTGAT AAATGATGTA GAAACTCCAA CATCAAGAAC ATATTCTAAT 
ACAAGATTAC AACATATTCT TTATTTTGAT AACAGATATT GGAAAAGACT GAGAAAAGAT 
ATTCAGAATG TGATCATTCC AACGTTGGCC TCTTCAAATT TATACAAGCC CATTTTCTGT 
CAACAGGTAG TCGAAATATT CAACCACATC ACAAGATCTG TGGCATATAT GGATAGAGAA 
CCTCAGCTAA CAGCTATAAG AGAGTGTGTC GTACAGTTAT TTACATGCCC TACGAATGCC 
AAAAATATTT TCGAAAACCA AAGTTTTTTG GATATTGTGT GGTCAATTAT AGACATTTTT 
AAGGAGTTTT GTAAGGTGGA AGGTGGTGTA TTGATCTGGC AGAGAGTTCA AAAAAGCAAT 
TTAACAAAGA GTTACAGTAT ATCATTCAAG CAAGGGTTGT ATACAGTTGA AACTTTATTG 
AGTAAGGTAC ATGATCCTAA TATTCCATTG AGACCTAAGG AAATAATATC TCTATTAACT 
CTTTGCAAGC TCTTCAATGG AGCGTGGAAG ATAAAGCGTA AAGAAGGTGA ACATGTTCTT 
CACGAAGATC AAAATTTCAT CTCCTATTTA GAATATACCA CCTCCATTTA CAGTATTATT 
CAAACAGCGG AAAAAGTTAG CGAAAAATCA AAAGATTCCA TTGATTCAAA GCTTTTTTTA 
AATGCAATAA GAATTATCAG TTCATTTCTA GGCAATAGAT CACTAACATA CAAGTTAATA 
TATGATTCTC ATGAAGTCAT TAAGTTTAGC GTAAGCCACG AAAGAGTTGC ATTTATGAAT 
CCTTTACAAA CAATGCTTTC ATTCCTTATC GAAAAAGTGT CTTTAAAGGA TGCATACGAA 
GCGTTAGAAG ACTGTTCAGA TTTTCTGAAA ATATCGGATT TCTCTTTGAG ATCAGTTGTA 
TTGTGCTCTC AAATTGACGT CGGGTTTTGG GTAAGAAATG GTATGTCTGT GTTACATCAA 
GCATCGTATT ATAAAAACAA TCCCGAATTA GGTTCCTATT CCAGAGATAT ACATTTGAAT 
CAGTTAGCTA TCCTTTGGGA ACGTGATGAC ATTCCGAGAA TAATATACAA TATTCTTGAT 
AGATGGGAAT TACTAGACTG GTTTACAGGT GAAGTTGACT ATCAACACAC TGTCTATGAG 
GATAAAATTT CATTCATCAT TCAACAATTC ATTGCCTTTA TTTATCAAAT ATTGACCGAA 
AGACAATATT TTAAAACTTT CTCTTCTCTA AAGGATAGAA GAATGGATCA AATCAAAAAT 
TCAATCATTT ATAACCTTTA CATGAAACCC CTTTCGTATT CAAAGCTACT AAGATCAGTT 
CCTGACTACC TAACTGAAGA TACTACCGAG TTTGACGAAG CACTTGAAGA AGTCTCTGTT 
TTCGTTGAGC CTAAGGGCTT GGCCGACAAT GGTGTTTTTA AATTAAAAGC AAGCTTGTAT 
GCAAAGGTTG ATCCGTTAAA GTTGCTGAAT TTGGAAAACG AATTCGAAAG TAGTGCAACT 
ATTATTAAAT CACATTTGGC TAAGGATAAG GATGAAATAG CAAAAGTTGT ACTAATACCC 
CAAGTCTCAA TAAAACAGTT AGATAAAGAC GCTCTCAATC TGGGTGCTTT TACAAGGAAT 
ACGGTATTTG CCAAAGTGGT CTATAAACTA CTCCAGGTAT GTCTGGATAT GGAGGATAGC 
ACTTTCCTGA ATGAGCTACT ACATTTAGTT CATGGTATAT TCAGGGATGA TGAATTGATA 
AATGGTAAGG ACTCTATACC AGAAGCTTAT TTGTCAAAGC CTATATGTAA TTTACTCTTG 
AGCATAGCCA ATGCTAAATC AGACGTATTT TCCGAGAGTA TTGTCCGTAA AGCCGACTAT 
TTGTTAGAGA AGATGATAAT GAAAAAACCA AATGAACTTT TTGAGTCATT AATTGCCAGT 
TTTGGCAACC AATACGTTAA TGATTATAAG GATAAGAAAC TACGCCAAGG AGTTAACTTA 
CAAGAGACAG AGAAGGAACG AAAAAGAAGA TTGGCCAAGA AACATCAAGC AAGGCTTCTA 
GCCAAGTTTA ACAATCAACA AACGAAATTC ATGAAAGAAC ATGAGTCTGA ATTTGATGAG 
CAGGATAACG ATGTTGACAT GGTCGGTGAA AAAGTATATG AATCGGAGGA TTTTACCTGC 
GCACTATGTC AAGATTCCAG TTCGACCGAT TTTTTTGTGA TACCTGCGTA CCATGATCAT 
TCCCCGATAT TTAGACCTGG TAATATTTTT AACCCAAATG AATTTATGCC CATGTGGGAT 
GGTTTCTACA ACGATGATGA AAAACAAGCT TATATTGACG ATGATGTTTT AGAGGCTTTG 
AAGGAAAACG GTAGTTGTGG ATCTAGGAAA GTATTTGTAT CCTGTAATCA TCACATTCAT 
CATAACTGCT TCAAGAGGTA CGTTCAAAAA AAGAGATTTT CATCAAACGC GTTTATATGT 
CCTTTATGCC AAACATTTTC GAATTGTACT TTACCTCTTT GTCAAACCTC AAAGGCAAAT 
ACTGGTTTGT CGCTAGATAT GTTTTTGGAA TCAGAACTTT CTTTGGATAC ATTGTCAAGG 
CTCTTCAAAC CTTTTACTGA GGAAAATTAT CGTACAATAA ACTCTATATT TTCGTTGATG 
ATATCTCAGT GTCAAGGTTT TGATAAAGCG GTAAGAAAGC GGGCCAACTT TTCGCATAAG 
GACGTTTCTT TAATATTAAG TGTGCACTGG GCAAATACCA TTTCAATGTT AGAAATTGCT 
TCTAGGTTAG AAAAGCCATA CAGTATTTCA TTTTTCAGAA GCAGGGAACA GAAATACAAA 
ACTTTAAAAA ATATATTAGT CTGCATTATG CTGTTCACCT TTGTCATAGG CAAGCCAAGT 
ATGGAGTTTG AACCGTACCC GCAGCAGCCC GATACTGTTT GGAACCAAAA TCAACTCTTC 
CAATATATTG TTCGAAGCGC TTTATTTTCT CCAGTTTCAC TTCGTCAGAC TGTTACAGAG 
GCGTTAACAA CGTTCTCTAG ACAGTTTCTA AGAGATTTTC TTCAGGGTCT TTCTGATGCA 
GAACAGGTTA CCAAATTGTA TGCTAAAGCG TCCAAAATTG GGGATGTTCT TAAAGTAAGT 
GAGCAGATGC TCTTCGCCCT AAGGACCATA TCTGATGTAC GAATGGAGGG ATTGGACTCC 
GAAAGTATTA TTTATGATCT AGCTTATACC TTCCTGTTGA AGAGTCTTCT TCCTACGATA 
AGAAGGTGCT TAGTCTTTAT CAAAGTGCTA CACGAATTAG TGAAAGATTC TGAAAATGAA 
ACGCTGGTCA TCAACGGTCA TGAGGTTGAA GAAGAACTGG AATTCGAAGA TACCGCCGAG 
TTTGTCAATA AGGCATTGAA AATGATTACT GAAAAAGAAT CCTTAGTCGA TCTTTTGACA 
ACGCAGGAGT CCATTGTATC GCATCCATAT TTGGAGAACA TCCCATATGA ATATTGTGGG 
ATTATAAAAC TGATAGATTT ATCAAAATAT TTGAATACTT ACGTTACCCA ATCTAAGGAA 
ATCAAGCTAC GTGAAGAGCG TTCTCAGCAT ATGAAGAATG CGGATAATCG ATTAGATTTC 
AAAATATGTT TGACATGTGG TGTCAAAGTG CATTTAAGAG CGGACCGCCA TGAAATGACC 
AAGCATTTGA ATAAAAATTG TTTTAAACCA TTTGGCGCCT TCCTGATGCC CAATTCAAGT 
GAAGTTTGTC TCCATTTGAC ACAGCCTCCT TCTAATATTT TTATATCTGC ACCATATCTA 
AATTCGCATG GTGAAGTTGG TAGAAATGCC ATGAGAAGAG GTGATTTAAC CACCTTGAAT 
TTGAAAAGAT ATGAGCACTT AAACAGATTA TGGATTAACA ATGAAATTCC AGGTTATATT 
AGTAGAGTAA TGGGTGACGA ATTTCGAGTG ACTATACTTT CCAATGGTTT CTTGTTTGCA 
TTTAATAGAG AACCAAGGCC AAGAAGAATA CCTCCAACAG ATGAAGATGA TGAAGATATG 
GAAGAGGGAG AAGACGGGTT TTTTACGGAG GGAAATGACG AAATGGATGT TGATGATGAA 
ACTGGACAGG CCGCCAACTT ATTTGGGGTT GGCGCAGAAG GTATTGCTGG TGGCGGAGTA 
CGTGATTTTT TCCAATTCTT CGAAAACTTT AGGAACACTC TACAACCGCA GGGAAATGGC 
GATGATGATG CACCTCAGAA TCCCCCACCA ATATTGCAGT TCTTAGGACC TCAATTTGAT 
GGAGCTACTA TCATAAGAAA TACAAATCCA AGAAACCTCG ATGAGGATGA CTCTGATGAT 
AATGATGACT CTGATGAGCG AGAGATTTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011700.1
CDS	1..5853
Translation	MSVADDDLGSLQGHIRRTLRSIHNLPYFRYTRGPTERADMSRALKEFIYRYLYFVISNSGENLPTLFNAHPKQKLSNPELTVFPDSLEDAVDIDKITSQQTIPFYKIDESRIGDVHKHTGRNCGRKFKIGEPLYRCHECGCDDTCVLCIHCFNPKDHVNHHVCTDICTEFTSGICDCGDEEAWNSPLHCKAEEQENDISEDPATNADIKEEDVWNDSVNIALVELVLAEVFDYFIDVFNQNIEPLPTIQKDITIKLREMTQQGKMYERAQFLNDLKYENDYMFDGTTTAKTSPSNSPEASPSLAKIDPENYTVIIYNDEYHNYSQATTALRQGVPDNVHIDLLTSRIDGEGRAMLKCSQDLSSVLGGFFAVQTNGLSATLTSWSEYLHQETCKYIILWITHCLNIPNSSFQTTFRNMMGKTLCSEYLNATECRDMTPVVEKYFSNKFDKNDPYRYIDLSILADGNQIPLGHHKILPESSTHSLSPLINDVETPTSRTYSNTRLQHILYFDNRYWKRLRKDIQNVIIPTLASSNLYKPIFCQQVVEIFNHITRSVAYMDREPQLTAIRECVVQLFTCPTNAKNIFENQSFLDIVWSIIDIFKEFCKVEGGVLIWQRVQKSNLTKSYSISFKQGLYTVETLLSKVHDPNIPLRPKEIISLLTLCKLFNGAWKIKRKEGEHVLHEDQNFISYLEYTTSIYSIIQTAEKVSEKSKDSIDSKLFLNAIRIISSFLGNRSLTYKLIYDSHEVIKFSVSHERVAFMNPLQTMLSFLIEKVSLKDAYEALEDCSDFLKISDFSLRSVVLCSQIDVGFWVRNGMSVLHQASYYKNNPELGSYSRDIHLNQLAILWERDDIPRIIYNILDRWELLDWFTGEVDYQHTVYEDKISFIIQQFIAFIYQILTERQYFKTFSSLKDRRMDQIKNSIIYNLYMKPLSYSKLLRSVPDYLTEDTTEFDEALEEVSVFVEPKGLADNGVFKLKASLYAKVDPLKLLNLENEFESSATIIKSHLAKDKDEIAKVVLIPQVSIKQLDKDALNLGAFTRNTVFAKVVYKLLQVCLDMEDSTFLNELLHLVHGIFRDDELINGKDSIPEAYLSKPICNLLLSIANAKSDVFSESIVRKADYLLEKMIMKKPNELFESLIASFGNQYVNDYKDKKLRQGVNLQETEKERKRRLAKKHQARLLAKFNNQQTKFMKEHESEFDEQDNDVDMVGEKVYESEDFTCALCQDSSSTDFFVIPAYHDHSPIFRPGNIFNPNEFMPMWDGFYNDDEKQAYIDDDVLEALKENGSCGSRKVFVSCNHHIHHNCFKRYVQKKRFSSNAFICPLCQTFSNCTLPLCQTSKANTGLSLDMFLESELSLDTLSRLFKPFTEENYRTINSIFSLMISQCQGFDKAVRKRANFSHKDVSLILSVHWANTISMLEIASRLEKPYSISFFRSREQKYKTLKNILVCIMLFTFVIGKPSMEFEPYPQQPDTVWNQNQLFQYIVRSALFSPVSLRQTVTEALTTFSRQFLRDFLQGLSDAEQVTKLYAKASKIGDVLKVSEQMLFALRTISDVRMEGLDSESIIYDLAYTFLLKSLLPTIRRCLVFIKVLHELVKDSENETLVINGHEVEEELEFEDTAEFVNKALKMITEKESLVDLLTTQESIVSHPYLENIPYEYCGIIKLIDLSKYLNTYVTQSKEIKLREERSQHMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKNCFKPFGAFLMPNSSEVCLHLTQPPSNIFISAPYLNSHGEVGRNAMRRGDLTTLNLKRYEHLNRLWINNEIPGYISRVMGDEFRVTILSNGFLFAFNREPRPRRIPPTDEDDEDMEEGEDGFFTEGNDEMDVDDETGQAANLFGVGAEGIAGGGVRDFFQFFENFRNTLQPQGNGDDDAPQNPPPILQFLGPQFDGATIIRNTNPRNLDEDDSDDNDDSDEREIW

Target ORF information:

RefSeq Version	NM_001181313.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase UBR1 (UBR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181313.1

ORF Insert Sequence:

ATGTCCGTTG CTGATGATGA TTTAGGATCT TTACAAGGTC ACATTAGGAG AACACTGAGG 
TCTATTCATA ACCTCCCCTA TTTTAGGTAT ACGAGAGGTC CTACTGAAAG GGCTGACATG 
AGCAGAGCCC TTAAAGAGTT CATTTACAGA TATCTATACT TTGTCATTTC TAACAGCGGA 
GAGAACTTAC CTACTTTATT CAATGCTCAT CCAAAACAAA AATTATCTAA CCCAGAGCTT 
ACTGTTTTTC CTGACAGTTT AGAAGATGCT GTGGATATTG ATAAGATAAC ATCTCAACAA 
ACTATTCCGT TTTATAAGAT AGATGAATCC AGAATAGGAG ACGTCCATAA ACATACCGGA 
AGAAATTGTG GGAGGAAATT CAAAATAGGG GAACCCTTGT ATAGGTGTCA TGAGTGTGGT 
TGCGATGATA CTTGTGTGCT TTGTATTCAT TGTTTTAATC CAAAAGATCA TGTGAATCAT 
CATGTTTGTA CCGATATATG TACTGAATTC ACTAGTGGAA TTTGTGACTG TGGAGATGAA 
GAAGCTTGGA ATTCTCCACT TCATTGCAAA GCTGAAGAAC AAGAAAACGA TATATCGGAA 
GATCCCGCCA CAAATGCCGA CATAAAGGAA GAAGATGTAT GGAATGATTC AGTGAATATA 
GCATTAGTGG AGTTGGTTCT TGCAGAAGTG TTTGACTATT TTATCGATGT TTTTAACCAG 
AATATCGAAC CTCTACCCAC AATACAGAAA GATATCACTA TCAAATTAAG AGAGATGACA 
CAACAGGGAA AAATGTATGA AAGAGCGCAG TTTTTAAACG ATTTAAAATA TGAAAACGAT 
TATATGTTTG ACGGAACAAC AACAGCAAAG ACGAGCCCCT CTAATAGCCC TGAGGCATCT 
CCATCGCTTG CGAAAATCGA TCCCGAAAAT TACACGGTAA TAATATATAA CGATGAATAT 
CATAACTATT CGCAGGCTAC GACTGCCTTG AGACAGGGGG TTCCAGATAA CGTACACATT 
GATCTATTAA CATCCAGGAT TGACGGAGAA GGTCGAGCAA TGTTGAAATG TTCACAAGAT 
TTATCATCTG TCTTAGGTGG TTTTTTTGCT GTTCAAACGA ATGGATTAAG TGCCACTTTA 
ACATCGTGGT CAGAATATCT TCATCAAGAA ACATGCAAGT ATATCATCCT TTGGATTACA 
CACTGTTTAA ACATACCAAA CTCCTCCTTC CAAACCACTT TTAGGAACAT GATGGGTAAA 
ACCCTGTGTT CAGAATACTT AAATGCAACA GAATGTCGTG ATATGACACC GGTGGTGGAG 
AAATATTTCA GCAACAAGTT CGATAAGAAT GATCCCTACA GATACATCGA TTTATCTATC 
CTTGCAGATG GCAATCAAAT TCCTTTGGGT CATCACAAAA TTCTACCCGA ATCAAGCACC 
CATTCACTTT CACCATTGAT AAATGATGTA GAAACTCCAA CATCAAGAAC ATATTCTAAT 
ACAAGATTAC AACATATTCT TTATTTTGAT AACAGATATT GGAAAAGACT GAGAAAAGAT 
ATTCAGAATG TGATCATTCC AACGTTGGCC TCTTCAAATT TATACAAGCC CATTTTCTGT 
CAACAGGTAG TCGAAATATT CAACCACATC ACAAGATCTG TGGCATATAT GGATAGAGAA 
CCTCAGCTAA CAGCTATAAG AGAGTGTGTC GTACAGTTAT TTACATGCCC TACGAATGCC 
AAAAATATTT TCGAAAACCA AAGTTTTTTG GATATTGTGT GGTCAATTAT AGACATTTTT 
AAGGAGTTTT GTAAGGTGGA AGGTGGTGTA TTGATCTGGC AGAGAGTTCA AAAAAGCAAT 
TTAACAAAGA GTTACAGTAT ATCATTCAAG CAAGGGTTGT ATACAGTTGA AACTTTATTG 
AGTAAGGTAC ATGATCCTAA TATTCCATTG AGACCTAAGG AAATAATATC TCTATTAACT 
CTTTGCAAGC TCTTCAATGG AGCGTGGAAG ATAAAGCGTA AAGAAGGTGA ACATGTTCTT 
CACGAAGATC AAAATTTCAT CTCCTATTTA GAATATACCA CCTCCATTTA CAGTATTATT 
CAAACAGCGG AAAAAGTTAG CGAAAAATCA AAAGATTCCA TTGATTCAAA GCTTTTTTTA 
AATGCAATAA GAATTATCAG TTCATTTCTA GGCAATAGAT CACTAACATA CAAGTTAATA 
TATGATTCTC ATGAAGTCAT TAAGTTTAGC GTAAGCCACG AAAGAGTTGC ATTTATGAAT 
CCTTTACAAA CAATGCTTTC ATTCCTTATC GAAAAAGTGT CTTTAAAGGA TGCATACGAA 
GCGTTAGAAG ACTGTTCAGA TTTTCTGAAA ATATCGGATT TCTCTTTGAG ATCAGTTGTA 
TTGTGCTCTC AAATTGACGT CGGGTTTTGG GTAAGAAATG GTATGTCTGT GTTACATCAA 
GCATCGTATT ATAAAAACAA TCCCGAATTA GGTTCCTATT CCAGAGATAT ACATTTGAAT 
CAGTTAGCTA TCCTTTGGGA ACGTGATGAC ATTCCGAGAA TAATATACAA TATTCTTGAT 
AGATGGGAAT TACTAGACTG GTTTACAGGT GAAGTTGACT ATCAACACAC TGTCTATGAG 
GATAAAATTT CATTCATCAT TCAACAATTC ATTGCCTTTA TTTATCAAAT ATTGACCGAA 
AGACAATATT TTAAAACTTT CTCTTCTCTA AAGGATAGAA GAATGGATCA AATCAAAAAT 
TCAATCATTT ATAACCTTTA CATGAAACCC CTTTCGTATT CAAAGCTACT AAGATCAGTT 
CCTGACTACC TAACTGAAGA TACTACCGAG TTTGACGAAG CACTTGAAGA AGTCTCTGTT 
TTCGTTGAGC CTAAGGGCTT GGCCGACAAT GGTGTTTTTA AATTAAAAGC AAGCTTGTAT 
GCAAAGGTTG ATCCGTTAAA GTTGCTGAAT TTGGAAAACG AATTCGAAAG TAGTGCAACT 
ATTATTAAAT CACATTTGGC TAAGGATAAG GATGAAATAG CAAAAGTTGT ACTAATACCC 
CAAGTCTCAA TAAAACAGTT AGATAAAGAC GCTCTCAATC TGGGTGCTTT TACAAGGAAT 
ACGGTATTTG CCAAAGTGGT CTATAAACTA CTCCAGGTAT GTCTGGATAT GGAGGATAGC 
ACTTTCCTGA ATGAGCTACT ACATTTAGTT CATGGTATAT TCAGGGATGA TGAATTGATA 
AATGGTAAGG ACTCTATACC AGAAGCTTAT TTGTCAAAGC CTATATGTAA TTTACTCTTG 
AGCATAGCCA ATGCTAAATC AGACGTATTT TCCGAGAGTA TTGTCCGTAA AGCCGACTAT 
TTGTTAGAGA AGATGATAAT GAAAAAACCA AATGAACTTT TTGAGTCATT AATTGCCAGT 
TTTGGCAACC AATACGTTAA TGATTATAAG GATAAGAAAC TACGCCAAGG AGTTAACTTA 
CAAGAGACAG AGAAGGAACG AAAAAGAAGA TTGGCCAAGA AACATCAAGC AAGGCTTCTA 
GCCAAGTTTA ACAATCAACA AACGAAATTC ATGAAAGAAC ATGAGTCTGA ATTTGATGAG 
CAGGATAACG ATGTTGACAT GGTCGGTGAA AAAGTATATG AATCGGAGGA TTTTACCTGC 
GCACTATGTC AAGATTCCAG TTCGACCGAT TTTTTTGTGA TACCTGCGTA CCATGATCAT 
TCCCCGATAT TTAGACCTGG TAATATTTTT AACCCAAATG AATTTATGCC CATGTGGGAT 
GGTTTCTACA ACGATGATGA AAAACAAGCT TATATTGACG ATGATGTTTT AGAGGCTTTG 
AAGGAAAACG GTAGTTGTGG ATCTAGGAAA GTATTTGTAT CCTGTAATCA TCACATTCAT 
CATAACTGCT TCAAGAGGTA CGTTCAAAAA AAGAGATTTT CATCAAACGC GTTTATATGT 
CCTTTATGCC AAACATTTTC GAATTGTACT TTACCTCTTT GTCAAACCTC AAAGGCAAAT 
ACTGGTTTGT CGCTAGATAT GTTTTTGGAA TCAGAACTTT CTTTGGATAC ATTGTCAAGG 
CTCTTCAAAC CTTTTACTGA GGAAAATTAT CGTACAATAA ACTCTATATT TTCGTTGATG 
ATATCTCAGT GTCAAGGTTT TGATAAAGCG GTAAGAAAGC GGGCCAACTT TTCGCATAAG 
GACGTTTCTT TAATATTAAG TGTGCACTGG GCAAATACCA TTTCAATGTT AGAAATTGCT 
TCTAGGTTAG AAAAGCCATA CAGTATTTCA TTTTTCAGAA GCAGGGAACA GAAATACAAA 
ACTTTAAAAA ATATATTAGT CTGCATTATG CTGTTCACCT TTGTCATAGG CAAGCCAAGT 
ATGGAGTTTG AACCGTACCC GCAGCAGCCC GATACTGTTT GGAACCAAAA TCAACTCTTC 
CAATATATTG TTCGAAGCGC TTTATTTTCT CCAGTTTCAC TTCGTCAGAC TGTTACAGAG 
GCGTTAACAA CGTTCTCTAG ACAGTTTCTA AGAGATTTTC TTCAGGGTCT TTCTGATGCA 
GAACAGGTTA CCAAATTGTA TGCTAAAGCG TCCAAAATTG GGGATGTTCT TAAAGTAAGT 
GAGCAGATGC TCTTCGCCCT AAGGACCATA TCTGATGTAC GAATGGAGGG ATTGGACTCC 
GAAAGTATTA TTTATGATCT AGCTTATACC TTCCTGTTGA AGAGTCTTCT TCCTACGATA 
AGAAGGTGCT TAGTCTTTAT CAAAGTGCTA CACGAATTAG TGAAAGATTC TGAAAATGAA 
ACGCTGGTCA TCAACGGTCA TGAGGTTGAA GAAGAACTGG AATTCGAAGA TACCGCCGAG 
TTTGTCAATA AGGCATTGAA AATGATTACT GAAAAAGAAT CCTTAGTCGA TCTTTTGACA 
ACGCAGGAGT CCATTGTATC GCATCCATAT TTGGAGAACA TCCCATATGA ATATTGTGGG 
ATTATAAAAC TGATAGATTT ATCAAAATAT TTGAATACTT ACGTTACCCA ATCTAAGGAA 
ATCAAGCTAC GTGAAGAGCG TTCTCAGCAT ATGAAGAATG CGGATAATCG ATTAGATTTC 
AAAATATGTT TGACATGTGG TGTCAAAGTG CATTTAAGAG CGGACCGCCA TGAAATGACC 
AAGCATTTGA ATAAAAATTG TTTTAAACCA TTTGGCGCCT TCCTGATGCC CAATTCAAGT 
GAAGTTTGTC TCCATTTGAC ACAGCCTCCT TCTAATATTT TTATATCTGC ACCATATCTA 
AATTCGCATG GTGAAGTTGG TAGAAATGCC ATGAGAAGAG GTGATTTAAC CACCTTGAAT 
TTGAAAAGAT ATGAGCACTT AAACAGATTA TGGATTAACA ATGAAATTCC AGGTTATATT 
AGTAGAGTAA TGGGTGACGA ATTTCGAGTG ACTATACTTT CCAATGGTTT CTTGTTTGCA 
TTTAATAGAG AACCAAGGCC AAGAAGAATA CCTCCAACAG ATGAAGATGA TGAAGATATG 
GAAGAGGGAG AAGACGGGTT TTTTACGGAG GGAAATGACG AAATGGATGT TGATGATGAA 
ACTGGACAGG CCGCCAACTT ATTTGGGGTT GGCGCAGAAG GTATTGCTGG TGGCGGAGTA 
CGTGATTTTT TCCAATTCTT CGAAAACTTT AGGAACACTC TACAACCGCA GGGAAATGGC 
GATGATGATG CACCTCAGAA TCCCCCACCA ATATTGCAGT TCTTAGGACC TCAATTTGAT 
GGAGCTACTA TCATAAGAAA TACAAATCCA AGAAACCTCG ATGAGGATGA CTCTGATGAT 
AATGATGACT CTGATGAGCG AGAGATTTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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UBR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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