CHO2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CHO2
Entrez Gene ID	853061
Full Name	phosphatidylethanolamine N-methyltransferase
Synonyms	PEM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181286.1	NP_011673.1	phosphatidylethanolamine N-methyltransferase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F18282g	XP_455900.1
Eremothecium gossypii	AGOS_AFR110C	NP_985657.1
Magnaporthe oryzae (rice blast fungus)	MGG_07110	XP_003715300.1
Saccharomyces cerevisiae (baker's yeast)	CHO2	NP_011673.1
Schizosaccharomyces pombe (fission yeast)	cho2	NP_596644.1
Neurospora crassa	NCU08045	XP_962715.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02379	NM_001181286.1 Latest version!	Saccharomyces cerevisiae S288C phosphatidylethanolamine N-methyltransferase (CHO2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHO2 ( NM_001181286.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011673.1 Saccharomyces cerevisiae S288C phosphatidylethanolamine N-methyltransferase (CHO2), partial mRNA.

Price & Availability↑

CloneID	OSi02379
Clone ID Related Accession (Same CDS sequence)	NM_001181286.1
Accession Version	NM_001181286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphatidylethanolamine N-methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCAGTT GTAAAACCAC TTTGTCTGAA ATGGTTGGTT CTGTGACAAA AGATAGGGGC 
ACTATCAACG TCGAAGCCAG AACACGTTCG AGTAATGTAA CTTTCAAACC ACCTGTAACC 
CACGATATGG TGCGTTCGCT TTTCGATCCA ACTTTGAAGA AATCTCTGCT GGAAAAATGC 
ATCGCGCTAG CTATTATATC GAATTTTTTC ATTTGTTATT GGGTCTTCCA AAGGTTTGGC 
TTACAGTTTA CCAAATACTT TTTTCTGGTA CAGTATTTAT TTTGGAGAAT TGCTTATAAT 
TTAGGTATTG GACTGGTTCT GCATTACCAG TCACATTATG AAACACTAAC GAATTGTGCT 
AAAACTCATG CGATTTTCAG CAAAATACCA CAGAATAAAG ATGCTAATTC GAATTTCTCA 
ACAAATTCCA ATTCCTTTTC AGAAAAATTT TGGAATTTTA TTAGGAAGTT CTGTCAATAT 
GAGATTAGGT CTAAAATGCC AAAGGAGTAT GATTTATTTG CCTATCCAGA AGAGATCAAC 
GTCTGGTTGA TATTTCGCCA GTTTGTCGAT TTAATCTTGA TGCAAGATTT TGTGACTTAC 
ATTATTTACG TTTACCTTTC TATTCCATAT AGCTGGGTTC AAATCTTCAA CTGGAGATCT 
TTGCTAGGTG TTATTTTGAT TTTATTTAAC ATCTGGGTCA AGTTAGATGC GCATCGCGTG 
GTTAAAGATT ATGCTTGGTA CTGGGGTGAT TTCTTCTTTT TGGAAGAATC TGAATTGATT 
TTTGATGGTG TCTTCAACAT CTCTCCACAT CCAATGTATT CTATTGGTTA TTTGGGTTAT 
TATGGCCTAT CATTGATTTG TAATGACTAC AAGGTTCTTC TGGTGTCCGT ATTTGGACAT 
TATTCTCAAT TTTTGTTTCT GAAATACGTC GAAAATCCTC ATATTGAAAG AACGTATGGT 
GATGGCACTG ATTCCGATTC TCAAATGAAT AGCAGAATTG ATGACTTGAT ATCAAAAGAG 
AATTACGATT ATTCAAGACC TCTAATTAAC ATGGGTCTTT CTTTTAACAA CTTCAATAAG 
CTAAGATTTA CTGACTATTT CACAATTGGT ACCGTAGCGG CACTTATGTT AGGAACAATC 
ATGAATGCTA GGTTTATCAA TTTAAATTAC CTCTTTATTA CCGTTTTTGT AACAAAACTG 
GTTTCATGGT TATTTATCTC GACAATATTG TACAAGCAAT CTCAGTCTAA ATGGTTCACG 
AGATTGTTTT TGGAGAATGG TTACACACAA GTTTATTCCT ATGAGCAATG GCAATTTATT 
TACAATTATT ATTTGGTATT AACCTACACA CTTATGATAA TTCATACAGG GCTTCAAATT 
TGGAGTAATT TTTCCAACAT AAACAACAGT CAATTAATTT TTGGTTTAAT TCTTGTGGCT 
TTACAAACAT GGTGTGATAA GGAAACGAGA CTAGCAATCT CTGATTTTGG TTGGTTTTAT 
GGTGATTTTT TCTTGAGCAA CTATATCTCA ACTAGAAAGC TGACTTCCCA AGGTATCTAC 
AGATATTTGA ATCACCCAGA AGCGGTATTA GGCGTTGTTG GAGTTTGGGG TACTGTATTG 
ATGACGAATT TTGCCGTTAC AAATATTATC TTGGCTGTTT TATGGACATT GACAAATTTT 
ATTCTTGTCA AGTTTATTGA AACACCTCAT GTTAATAAGA TATATGGTAA AACTAAACGA 
GTTAGTGGTG TTGGAAAAAC TTTACTAGGC TTGAAACCTC TAAGACAAGT TTCAGATATT 
GTCAATAGAA TTGAAAATAT TATAATCAAA TCATTAGTTG ATGAAAGCAA GAACTCCAAT 
GGTGGGGCAG AACTTTTGCC CAAAAATTAT CAAGATAACA AAGAATGGAA TATTTTAATT 
CAGGAAGCAA TGGATAGCGT TGCAACAAGA TTGAGTCCAT ACTGTGAATT GAAAATTGAA 
AACGAGCAGG TAGAAACCAA TTTTGTCTTG CCTACTCCAG TAACTTTGAA TTGGAAAATG 
CCCATTGAAC TTTATAACGG AGATGATTGG ATTGGTTTGT ATAAAGTCAT TGATACAAGG 
GCAGATCGTG AAAAAACAAG AGTTGGATCA GGTGGTCATT GGAGTGCGAC TTCTAAAGAT 
TCATATATGA ACCATGGATT AAGACATAAA GAATCTGTAA CAGAAATAAA GGCGACTGAA 
AAGTACGTTC AAGGTAAAGT GACATTCGAC ACGTCTTTGC TTTATTTTGA AAATGGTATC 
TACGAATTTA GGTATCATTC TGGTAACTCC CATAAGGTGT TATTGATTTC TACACCATTT 
GAAATTTCAT TGCCGGTACT AAATACCACG ACACCTGAAC TGTTTGAGAA GGATTTGACT 
GAGTTTTTGA CGAAAGTCAA CGTTTTGAAG GATGGTAAAT TTCGTCCATT AGGAAATAAG 
TTCTTCGGGA TGGACAGCTT GAAACAATTG ATTAAGAACT CAATTGGCGT CGAACTTTCG 
TCTGAGTATA TGAGGAGAGT CAATGGTGAC GCTCACGTTA TTTCGCATCG CGCTTGGGAT 
ATAAAACAAA CGCTTGATAG TCTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011673.1
CDS	1..2610
Translation	MSSCKTTLSEMVGSVTKDRGTINVEARTRSSNVTFKPPVTHDMVRSLFDPTLKKSLLEKCIALAIISNFFICYWVFQRFGLQFTKYFFLVQYLFWRIAYNLGIGLVLHYQSHYETLTNCAKTHAIFSKIPQNKDANSNFSTNSNSFSEKFWNFIRKFCQYEIRSKMPKEYDLFAYPEEINVWLIFRQFVDLILMQDFVTYIIYVYLSIPYSWVQIFNWRSLLGVILILFNIWVKLDAHRVVKDYAWYWGDFFFLEESELIFDGVFNISPHPMYSIGYLGYYGLSLICNDYKVLLVSVFGHYSQFLFLKYVENPHIERTYGDGTDSDSQMNSRIDDLISKENYDYSRPLINMGLSFNNFNKLRFTDYFTIGTVAALMLGTIMNARFINLNYLFITVFVTKLVSWLFISTILYKQSQSKWFTRLFLENGYTQVYSYEQWQFIYNYYLVLTYTLMIIHTGLQIWSNFSNINNSQLIFGLILVALQTWCDKETRLAISDFGWFYGDFFLSNYISTRKLTSQGIYRYLNHPEAVLGVVGVWGTVLMTNFAVTNIILAVLWTLTNFILVKFIETPHVNKIYGKTKRVSGVGKTLLGLKPLRQVSDIVNRIENIIIKSLVDESKNSNGGAELLPKNYQDNKEWNILIQEAMDSVATRLSPYCELKIENEQVETNFVLPTPVTLNWKMPIELYNGDDWIGLYKVIDTRADREKTRVGSGGHWSATSKDSYMNHGLRHKESVTEIKATEKYVQGKVTFDTSLLYFENGIYEFRYHSGNSHKVLLISTPFEISLPVLNTTTPELFEKDLTEFLTKVNVLKDGKFRPLGNKFFGMDSLKQLIKNSIGVELSSEYMRRVNGDAHVISHRAWDIKQTLDSLA

Target ORF information:

RefSeq Version	NM_001181286.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphatidylethanolamine N-methyltransferase (CHO2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181286.1

ORF Insert Sequence:

ATGTCCAGTT GTAAAACCAC TTTGTCTGAA ATGGTTGGTT CTGTGACAAA AGATAGGGGC 
ACTATCAACG TCGAAGCCAG AACACGTTCG AGTAATGTAA CTTTCAAACC ACCTGTAACC 
CACGATATGG TGCGTTCGCT TTTCGATCCA ACTTTGAAGA AATCTCTGCT GGAAAAATGC 
ATCGCGCTAG CTATTATATC GAATTTTTTC ATTTGTTATT GGGTCTTCCA AAGGTTTGGC 
TTACAGTTTA CCAAATACTT TTTTCTGGTA CAGTATTTAT TTTGGAGAAT TGCTTATAAT 
TTAGGTATTG GACTGGTTCT GCATTACCAG TCACATTATG AAACACTAAC GAATTGTGCT 
AAAACTCATG CGATTTTCAG CAAAATACCA CAGAATAAAG ATGCTAATTC GAATTTCTCA 
ACAAATTCCA ATTCCTTTTC AGAAAAATTT TGGAATTTTA TTAGGAAGTT CTGTCAATAT 
GAGATTAGGT CTAAAATGCC AAAGGAGTAT GATTTATTTG CCTATCCAGA AGAGATCAAC 
GTCTGGTTGA TATTTCGCCA GTTTGTCGAT TTAATCTTGA TGCAAGATTT TGTGACTTAC 
ATTATTTACG TTTACCTTTC TATTCCATAT AGCTGGGTTC AAATCTTCAA CTGGAGATCT 
TTGCTAGGTG TTATTTTGAT TTTATTTAAC ATCTGGGTCA AGTTAGATGC GCATCGCGTG 
GTTAAAGATT ATGCTTGGTA CTGGGGTGAT TTCTTCTTTT TGGAAGAATC TGAATTGATT 
TTTGATGGTG TCTTCAACAT CTCTCCACAT CCAATGTATT CTATTGGTTA TTTGGGTTAT 
TATGGCCTAT CATTGATTTG TAATGACTAC AAGGTTCTTC TGGTGTCCGT ATTTGGACAT 
TATTCTCAAT TTTTGTTTCT GAAATACGTC GAAAATCCTC ATATTGAAAG AACGTATGGT 
GATGGCACTG ATTCCGATTC TCAAATGAAT AGCAGAATTG ATGACTTGAT ATCAAAAGAG 
AATTACGATT ATTCAAGACC TCTAATTAAC ATGGGTCTTT CTTTTAACAA CTTCAATAAG 
CTAAGATTTA CTGACTATTT CACAATTGGT ACCGTAGCGG CACTTATGTT AGGAACAATC 
ATGAATGCTA GGTTTATCAA TTTAAATTAC CTCTTTATTA CCGTTTTTGT AACAAAACTG 
GTTTCATGGT TATTTATCTC GACAATATTG TACAAGCAAT CTCAGTCTAA ATGGTTCACG 
AGATTGTTTT TGGAGAATGG TTACACACAA GTTTATTCCT ATGAGCAATG GCAATTTATT 
TACAATTATT ATTTGGTATT AACCTACACA CTTATGATAA TTCATACAGG GCTTCAAATT 
TGGAGTAATT TTTCCAACAT AAACAACAGT CAATTAATTT TTGGTTTAAT TCTTGTGGCT 
TTACAAACAT GGTGTGATAA GGAAACGAGA CTAGCAATCT CTGATTTTGG TTGGTTTTAT 
GGTGATTTTT TCTTGAGCAA CTATATCTCA ACTAGAAAGC TGACTTCCCA AGGTATCTAC 
AGATATTTGA ATCACCCAGA AGCGGTATTA GGCGTTGTTG GAGTTTGGGG TACTGTATTG 
ATGACGAATT TTGCCGTTAC AAATATTATC TTGGCTGTTT TATGGACATT GACAAATTTT 
ATTCTTGTCA AGTTTATTGA AACACCTCAT GTTAATAAGA TATATGGTAA AACTAAACGA 
GTTAGTGGTG TTGGAAAAAC TTTACTAGGC TTGAAACCTC TAAGACAAGT TTCAGATATT 
GTCAATAGAA TTGAAAATAT TATAATCAAA TCATTAGTTG ATGAAAGCAA GAACTCCAAT 
GGTGGGGCAG AACTTTTGCC CAAAAATTAT CAAGATAACA AAGAATGGAA TATTTTAATT 
CAGGAAGCAA TGGATAGCGT TGCAACAAGA TTGAGTCCAT ACTGTGAATT GAAAATTGAA 
AACGAGCAGG TAGAAACCAA TTTTGTCTTG CCTACTCCAG TAACTTTGAA TTGGAAAATG 
CCCATTGAAC TTTATAACGG AGATGATTGG ATTGGTTTGT ATAAAGTCAT TGATACAAGG 
GCAGATCGTG AAAAAACAAG AGTTGGATCA GGTGGTCATT GGAGTGCGAC TTCTAAAGAT 
TCATATATGA ACCATGGATT AAGACATAAA GAATCTGTAA CAGAAATAAA GGCGACTGAA 
AAGTACGTTC AAGGTAAAGT GACATTCGAC ACGTCTTTGC TTTATTTTGA AAATGGTATC 
TACGAATTTA GGTATCATTC TGGTAACTCC CATAAGGTGT TATTGATTTC TACACCATTT 
GAAATTTCAT TGCCGGTACT AAATACCACG ACACCTGAAC TGTTTGAGAA GGATTTGACT 
GAGTTTTTGA CGAAAGTCAA CGTTTTGAAG GATGGTAAAT TTCGTCCATT AGGAAATAAG 
TTCTTCGGGA TGGACAGCTT GAAACAATTG ATTAAGAACT CAATTGGCGT CGAACTTTCG 
TCTGAGTATA TGAGGAGAGT CAATGGTGAC GCTCACGTTA TTTCGCATCG CGCTTGGGAT 
ATAAAACAAA CGCTTGATAG TCTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CHO2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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