SKN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SKN1
Entrez Gene ID	853045
Full Name	beta-glucan synthesis-associated protein SKN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181272.3	NP_011659.3	beta-glucan synthesis-associated protein SKN1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E03697g	XP_454111.1
Saccharomyces cerevisiae (baker's yeast)	KRE6	NP_015485.1
Schizosaccharomyces pombe (fission yeast)	SPAC17G6.11c	NP_594258.1
Schizosaccharomyces pombe (fission yeast)	SPAC23H3.11c	NP_593801.1
Eremothecium gossypii	AGOS_AFR545W	NP_986092.1
Saccharomyces cerevisiae (baker's yeast)	SKN1	NP_011659.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process.
Title: Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae.

SKN1 is involved in sphingolipid biosynthesis in S. cerevisiae.
Title: SKN1, a novel plant defensin-sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis.

The following SKN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02366	NM_001181272.3 Latest version!	Saccharomyces cerevisiae S288C beta-glucan synthesis-associated protein SKN1 (SKN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKN1 ( NM_001181272.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011659.3 Saccharomyces cerevisiae S288C beta-glucan synthesis-associated protein SKN1 (SKN1), partial mRNA.

Price & Availability↑

CloneID	OSi02366
Clone ID Related Accession (Same CDS sequence)	NM_001181272.3
Accession Version	NM_001181272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	beta-glucan synthesis-associated protein SKN1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181272.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGTGC GAAACCTCAC CAATAATCGT CATTCCAACA GCGAAAATAG TGTTTCCGGA 
AGCGAAAACT CCTTTTATTC CTCGAATGAG CAATCAAGAC AAAGCTCATC ATTAGAACCT 
GCAGATGGTC AGAACGTTAG GGTGAGCGGT AATCCTTTCC TTGGAAGTGA AGAATTTGAC 
GAAGATTATA ATTCACCCAG TGGAGACGAT GAGCGGCGTG GGGCAAATGA ATATTCCAGT 
TCTTCATCCA TAAATTATAA TAACGATCCT AACAGCGATA CTTCTCTTCT GGCTAATGAA 
AAGAACTCAC CCGAGAGAAA TGGTCAAAGA ATGTCAGACT ATAAGGGCTA TTATGCTAAG 
ACTAACCTAA CTTCTGCTAA TAACTTAAAC AATCATAATA ACAATAATTA TAAGAACATT 
ATAAGCAGTA GTAACGATAA TAGTTTTGCA TCACATTTAC AGCCGCCGGA CAGAAACTTA 
CCGTCTCATC CTTCTTCAAA TAATATGAGT AGCTTTTCTA ACAACAGCTT AATTAAGTCT 
CCACCACCTT TTGACAGATA TCCGCTAGTA GGAACGAGGC ATATTTCAGC GGCTCAATCT 
CAGTCGCAGA ACCTTATAAA TGAAAAGAAA AGAGCGAATA TGACTGGCTC ATCTTCTTCT 
GCTCACGACT CTTCATTATC ATCCACCAAC TTATACATGG GCGAACAGGA TTTTTCGCCC 
TTTGGCGGTT ATCCAGCTTC CTTCTTTCCG TTAACGTTGG ACGAAAAGGA AGACGATGAC 
TACATTCATA ATCCAAATGT CGAGGAAGAA GCAAAGTTAG ATAGAAGGAG GTTCGTTGAC 
GACTTCAAAC ACATGGATAG AAGATCTTTC TTGGGGCTTC TGGGTATTCT ATTTTTATTT 
ATGGCTGGTA TATTTATTTT CATTGTATTA CCTGCAATTA CATTTTCCGG GGTAGTTTAT 
CATCATGAAC ATGTTCATGC GGCAAATTCA GCAGGATCGT CGTCTTCTAA TACTACATCC 
AAGTCATTGA CTGAATATCA ATATCCTCAA CTTGCGGCTA TAAGGACAAC TTTAGTTGAT 
CCAGACACAC CTGACAGTGC GAAGACAAGA GTAGCGAAAG ATGGATCTAA ATGGCAGCTG 
GTGTTTTCCG ATGAATTTAA TGCTGAAGGT AGGACCTTTT ACGATGGCGA TGACCAGTTT 
TGGACAGCTC CTGACATCCA CTACGACGCT ACGAAAGATC TTGAATGGTA CTCACCAGAT 
GCCGTTACAA CTACAAATGG TACCTTGACG TTGAGAATGG ATGCATTCAG AAACCATGAC 
CTTTATTACA GATCTGGTAT GGTACAGAGC TGGAACAAAC TCTGCTTTAC AGAAGGTGCG 
TTAGAAGTTT CCGCGAACCT CCCTAATTAT GGTCGAGTTA CAGGATTATG GCCTGGTATG 
TGGACCATGG GGAACTTGGG CAGACCAGGT TATTTGGCGA GTACTCAAGG TGTTTGGCCT 
TATTCATATG AAGCATGTGA CGCTGGTATA ACACCAAACC AAAGTTCCCC AGATGGTATT 
TCTTACTTGC CGGGACAAAA GTTGAGTGTT TGTACTTGTG ATAATGAGGA TCATCCAAAT 
CAGGGAGTTG GAAGAGGCGC ACCGGAAATA GATATTTTAG AAGGTGAAGC TGATACAATT 
TTGGGTGTTG GTGTTGCGTC ACAATCTCTA CAAATTGCTC CATTTGATAT ATGGTACATG 
CCCGATTATG ACTTCATCGA AGTTTATAAT TTCACCACAA CAACTATGAA TACTTACGCT 
GGTGGGCCTT TTCAACAAGC TGTCTCAGCA ATATCCACTT TAAATGTAAC ATGGTATGAA 
TTTGGGGAGG AAGCAGGTTA TTTTCAAAAG TATGCCATTG AATATCTCAA TGATGACGAC 
AACGGATATA TTCGTTGGTT TGTGGGAGAG AACCCTACTT TCACACTATA TGCTACTTCT 
TTACATCCTA GTGGTAATAT TGACTGGAGG AGAATAAGTA AGGAGCCAAT GTCAGCGATT 
TTAAACTTAG GTATTTCCAA CAACTGGGCC TATATCGACT GGCAATATAT ATTTTTCCCG 
GTGACAATGT CAATAGATTA CGTCCGATTA TACCAGCCGA AGGGTTCTAC GTCCATTACT 
TGTGATCCTG AGGACTACCC GACATATGAT TATATCCAAA GCCATCTAAA TGCATATTAT 
AACGCCAATT TAACTGATTG GGAACAGGCT GGATATACTT TCCCAAAAAA TATTCTAACA 
GGAGGTTGTA GTAGTTCAAA ATTCTCATTA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011659.3
CDS	1..2316
Translation	MSVRNLTNNRHSNSENSVSGSENSFYSSNEQSRQSSSLEPADGQNVRVSGNPFLGSEEFDEDYNSPSGDDERRGANEYSSSSSINYNNDPNSDTSLLANEKNSPERNGQRMSDYKGYYAKTNLTSANNLNNHNNNNYKNIISSSNDNSFASHLQPPDRNLPSHPSSNNMSSFSNNSLIKSPPPFDRYPLVGTRHISAAQSQSQNLINEKKRANMTGSSSSAHDSSLSSTNLYMGEQDFSPFGGYPASFFPLTLDEKEDDDYIHNPNVEEEAKLDRRRFVDDFKHMDRRSFLGLLGILFLFMAGIFIFIVLPAITFSGVVYHHEHVHAANSAGSSSSNTTSKSLTEYQYPQLAAIRTTLVDPDTPDSAKTRVAKDGSKWQLVFSDEFNAEGRTFYDGDDQFWTAPDIHYDATKDLEWYSPDAVTTTNGTLTLRMDAFRNHDLYYRSGMVQSWNKLCFTEGALEVSANLPNYGRVTGLWPGMWTMGNLGRPGYLASTQGVWPYSYEACDAGITPNQSSPDGISYLPGQKLSVCTCDNEDHPNQGVGRGAPEIDILEGEADTILGVGVASQSLQIAPFDIWYMPDYDFIEVYNFTTTTMNTYAGGPFQQAVSAISTLNVTWYEFGEEAGYFQKYAIEYLNDDDNGYIRWFVGENPTFTLYATSLHPSGNIDWRRISKEPMSAILNLGISNNWAYIDWQYIFFPVTMSIDYVRLYQPKGSTSITCDPEDYPTYDYIQSHLNAYYNANLTDWEQAGYTFPKNILTGGCSSSKFSLS

Target ORF information:

RefSeq Version	NM_001181272.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C beta-glucan synthesis-associated protein SKN1 (SKN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181272.3

ORF Insert Sequence:

ATGTCTGTGC GAAACCTCAC CAATAATCGT CATTCCAACA GCGAAAATAG TGTTTCCGGA 
AGCGAAAACT CCTTTTATTC CTCGAATGAG CAATCAAGAC AAAGCTCATC ATTAGAACCT 
GCAGATGGTC AGAACGTTAG GGTGAGCGGT AATCCTTTCC TTGGAAGTGA AGAATTTGAC 
GAAGATTATA ATTCACCCAG TGGAGACGAT GAGCGGCGTG GGGCAAATGA ATATTCCAGT 
TCTTCATCCA TAAATTATAA TAACGATCCT AACAGCGATA CTTCTCTTCT GGCTAATGAA 
AAGAACTCAC CCGAGAGAAA TGGTCAAAGA ATGTCAGACT ATAAGGGCTA TTATGCTAAG 
ACTAACCTAA CTTCTGCTAA TAACTTAAAC AATCATAATA ACAATAATTA TAAGAACATT 
ATAAGCAGTA GTAACGATAA TAGTTTTGCA TCACATTTAC AGCCGCCGGA CAGAAACTTA 
CCGTCTCATC CTTCTTCAAA TAATATGAGT AGCTTTTCTA ACAACAGCTT AATTAAGTCT 
CCACCACCTT TTGACAGATA TCCGCTAGTA GGAACGAGGC ATATTTCAGC GGCTCAATCT 
CAGTCGCAGA ACCTTATAAA TGAAAAGAAA AGAGCGAATA TGACTGGCTC ATCTTCTTCT 
GCTCACGACT CTTCATTATC ATCCACCAAC TTATACATGG GCGAACAGGA TTTTTCGCCC 
TTTGGCGGTT ATCCAGCTTC CTTCTTTCCG TTAACGTTGG ACGAAAAGGA AGACGATGAC 
TACATTCATA ATCCAAATGT CGAGGAAGAA GCAAAGTTAG ATAGAAGGAG GTTCGTTGAC 
GACTTCAAAC ACATGGATAG AAGATCTTTC TTGGGGCTTC TGGGTATTCT ATTTTTATTT 
ATGGCTGGTA TATTTATTTT CATTGTATTA CCTGCAATTA CATTTTCCGG GGTAGTTTAT 
CATCATGAAC ATGTTCATGC GGCAAATTCA GCAGGATCGT CGTCTTCTAA TACTACATCC 
AAGTCATTGA CTGAATATCA ATATCCTCAA CTTGCGGCTA TAAGGACAAC TTTAGTTGAT 
CCAGACACAC CTGACAGTGC GAAGACAAGA GTAGCGAAAG ATGGATCTAA ATGGCAGCTG 
GTGTTTTCCG ATGAATTTAA TGCTGAAGGT AGGACCTTTT ACGATGGCGA TGACCAGTTT 
TGGACAGCTC CTGACATCCA CTACGACGCT ACGAAAGATC TTGAATGGTA CTCACCAGAT 
GCCGTTACAA CTACAAATGG TACCTTGACG TTGAGAATGG ATGCATTCAG AAACCATGAC 
CTTTATTACA GATCTGGTAT GGTACAGAGC TGGAACAAAC TCTGCTTTAC AGAAGGTGCG 
TTAGAAGTTT CCGCGAACCT CCCTAATTAT GGTCGAGTTA CAGGATTATG GCCTGGTATG 
TGGACCATGG GGAACTTGGG CAGACCAGGT TATTTGGCGA GTACTCAAGG TGTTTGGCCT 
TATTCATATG AAGCATGTGA CGCTGGTATA ACACCAAACC AAAGTTCCCC AGATGGTATT 
TCTTACTTGC CGGGACAAAA GTTGAGTGTT TGTACTTGTG ATAATGAGGA TCATCCAAAT 
CAGGGAGTTG GAAGAGGCGC ACCGGAAATA GATATTTTAG AAGGTGAAGC TGATACAATT 
TTGGGTGTTG GTGTTGCGTC ACAATCTCTA CAAATTGCTC CATTTGATAT ATGGTACATG 
CCCGATTATG ACTTCATCGA AGTTTATAAT TTCACCACAA CAACTATGAA TACTTACGCT 
GGTGGGCCTT TTCAACAAGC TGTCTCAGCA ATATCCACTT TAAATGTAAC ATGGTATGAA 
TTTGGGGAGG AAGCAGGTTA TTTTCAAAAG TATGCCATTG AATATCTCAA TGATGACGAC 
AACGGATATA TTCGTTGGTT TGTGGGAGAG AACCCTACTT TCACACTATA TGCTACTTCT 
TTACATCCTA GTGGTAATAT TGACTGGAGG AGAATAAGTA AGGAGCCAAT GTCAGCGATT 
TTAAACTTAG GTATTTCCAA CAACTGGGCC TATATCGACT GGCAATATAT ATTTTTCCCG 
GTGACAATGT CAATAGATTA CGTCCGATTA TACCAGCCGA AGGGTTCTAC GTCCATTACT 
TGTGATCCTG AGGACTACCC GACATATGAT TATATCCAAA GCCATCTAAA TGCATATTAT 
AACGCCAATT TAACTGATTG GGAACAGGCT GGATATACTT TCCCAAAAAA TATTCTAACA 
GGAGGTTGTA GTAGTTCAAA ATTCTCATTA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SKN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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